| microbial monoterpene transformations-a review. | isoprene and monoterpenes constitute a significant fraction of new plant biomass. emission rates into the atmosphere alone are estimated to be over 500 tg per year. these natural hydrocarbons are mineralized annually in similar quantities. in the atmosphere, abiotic photochemical processes cause lifetimes of minutes to hours. microorganisms encounter isoprene, monoterpenes, and other volatiles of plant origin while living in and on plants, in the soil and in aquatic habitats. below toxic concent ... | 2014 | 25076942 |
| connecting lignin-degradation pathway with pre-treatment inhibitor sensitivity of cupriavidus necator. | to produce lignocellulosic biofuels economically, the complete release of monomers from the plant cell wall components, cellulose, hemicellulose, and lignin, through pre-treatment and hydrolysis (both enzymatic and chemical), and the efficient utilization of these monomers as carbon sources, is crucial. in addition, the identification and development of robust microbial biofuel production strains that can tolerate the toxic compounds generated during pre-treatment and hydrolysis is also essentia ... | 2014 | 24904560 |
| pseudomonas putida csv86: a candidate genome for genetic bioaugmentation. | pseudomonas putida csv86, a plasmid-free strain possessing capability to transfer the naphthalene degradation property, has been explored for its metabolic diversity through genome sequencing. the analysis of draft genome sequence of csv86 (6.4 mb) revealed the presence of genes involved in the degradation of naphthalene, salicylate, benzoate, benzylalcohol, p-hydroxybenzoate, phenylacetate and p-hydroxyphenylacetate on the chromosome thus ensuring the stability of the catabolic potential. moreo ... | 2014 | 24475028 |
| diversity of pseudomonas genomes, including populus-associated isolates, as revealed by comparative genome analysis. | the pseudomonas genus contains a metabolically versatile group of organisms that are known to occupy numerous ecological niches, including the rhizosphere and endosphere of many plants. their diversity influences the phylogenetic diversity and heterogeneity of these communities. on the basis of average amino acid identity, comparative genome analysis of >1,000 pseudomonas genomes, including 21 pseudomonas strains isolated from the roots of native populus deltoides (eastern cottonwood) trees resu ... | 2015 | 26519390 |
| diversity of pseudomonas genomes, including populus-associated isolates, as revealed by comparative genome analysis. | the pseudomonas genus contains a metabolically versatile group of organisms that are known to occupy numerous ecological niches, including the rhizosphere and endosphere of many plants. their diversity influences the phylogenetic diversity and heterogeneity of these communities. on the basis of average amino acid identity, comparative genome analysis of >1,000 pseudomonas genomes, including 21 pseudomonas strains isolated from the roots of native populus deltoides (eastern cottonwood) trees resu ... | 2015 | 26519390 |
| fleq dna binding consensus sequence revealed by studies of fleq-dependent regulation of biofilm gene expression in pseudomonas aeruginosa. | the transcription factor fleq from pseudomonas aeruginosa derepresses expression of genes involved in biofilm formation when intracellular levels of the second messenger cyclic diguanosine monophosphate (c-di-gmp) are high. fleq also activates transcription of flagellar genes, and the expression of these genes is highest at low intracellular c-di-gmp. fleq thus plays a central role in mediating the transition between planktonic and biofilm lifestyles of p. aeruginosa. previous work showed that f ... | 2015 | 26483521 |
| fleq dna binding consensus sequence revealed by studies of fleq-dependent regulation of biofilm gene expression in pseudomonas aeruginosa. | the transcription factor fleq from pseudomonas aeruginosa derepresses expression of genes involved in biofilm formation when intracellular levels of the second messenger cyclic diguanosine monophosphate (c-di-gmp) are high. fleq also activates transcription of flagellar genes, and the expression of these genes is highest at low intracellular c-di-gmp. fleq thus plays a central role in mediating the transition between planktonic and biofilm lifestyles of p. aeruginosa. previous work showed that f ... | 2015 | 26483521 |
| different ancestries of r tailocins in rhizospheric pseudomonas isolates. | bacterial genomes accommodate a variety of mobile genetic elements, including bacteriophage-related clusters that encode phage tail-like protein complexes playing a role in interactions with eukaryotic or prokaryotic cells. such tailocins are unable to replicate inside target cells due to the lack of a phage head with associated dna. a subset of tailocins mediate antagonistic activities with bacteriocin-like specificity. functional characterization of bactericidal tailocins of two pseudomonas pu ... | 2015 | 26412856 |
| bioenergetics and the role of soluble cytochromes c for alkaline adaptation in gram-negative alkaliphilic pseudomonas. | very few studies have been conducted on alkaline adaptation of gram-negative alkaliphiles. the reversed difference of h(+) concentration across the membrane will make energy production considerably difficult for gram-negative as well as gram-positive bacteria. cells of the alkaliphilic gram-negative bacterium pseudomonas alcaliphila al15-21(t) grown at ph 10 under low-aeration intensity have a soluble cytochrome c content that is 3.6-fold higher than that of the cells grown at ph 7 under high-ae ... | 2015 | 25705691 |
| screen of non-annotated small secreted proteins of pseudomonas syringae reveals a virulence factor that inhibits tomato immune proteases. | pseudomonas syringae pv. tomato dc3000 (ptodc3000) is an extracellular model plant pathogen, yet its potential to produce secreted effectors that manipulate the apoplast has been under investigated. here we identified 131 candidate small, secreted, non-annotated proteins from the ptodc3000 genome, most of which are common to pseudomonas species and potentially expressed during apoplastic colonization. we produced 43 of these proteins through a custom-made gateway-compatible expression system for ... | 2016 | 27603016 |
| the interaction between arbuscular mycorrhizal fungi and endophytic bacteria enhances plant growth of acacia gerrardii under salt stress. | microbes living symbiotically in plant tissues mutually cooperate with each other by providing nutrients for proliferation of the partner organism and have a beneficial effect on plant growth. however, few studies thus far have examined the interactive effect of endophytic bacteria and arbuscular mycorrhizal fungi (amf) in hostile conditions and their potential to improve plant stress tolerance. in this study, we investigated how the synergistic interactions of endophytic bacteria and amf affect ... | 2016 | 27486442 |
| gcsr, a tyrr-like enhancer-binding protein, regulates expression of the glycine cleavage system in pseudomonas aeruginosa pao1. | glycine serves as a major source of single carbon units for biochemical reactions within bacterial cells. utilization of glycine is tightly regulated and revolves around a key group of proteins known as the glycine cleavage system (gcs). our lab previously identified the transcriptional regulator gcsr (pa2449) as being required for catabolism of glycine in the opportunistic pathogen pseudomonas aeruginosa pao1. in an effort to clarify and have an overall better understanding of the role of gcsr ... | 2016 | 27303730 |
| characterization of the pseudomonas aeruginosa metalloendopeptidase, mep72, a member of the vfr regulon. | pseudomonas aeruginosa vfr (the virulence factor regulator) enhances p. aeruginosa virulence by positively regulating the expression of numerous virulence genes. a previous microarray analysis identified numerous genes positively regulated by vfr in strain pak, including the yet uncharacterized pa2782 and pa2783. | 2013 | 24279383 |
| linking toluene degradation with specific microbial populations in soil. | phospholipid fatty acid (plfa) analysis of a soil microbial community was coupled with (13)c isotope tracer analysis to measure the community's response to addition of 35 microg of [(13)c]toluene ml of soil solution(-1). after 119 h of incubation with toluene, 96% of the incorporated (13)c was detected in only 16 of the total 59 plfas (27%) extracted from the soil. of the total (13)c-enriched plfas, 85% were identical to the plfas contained in a toluene-metabolizing bacterium isolated from the s ... | 1999 | 10583996 |
| structural consequences of effector protein complex formation in a diiron hydroxylase. | carboxylate-bridged diiron hydroxylases are multicomponent enzyme complexes responsible for the catabolism of a wide range of hydrocarbons and as such have drawn attention for their mechanism of action and potential uses in bioremediation and enzymatic synthesis. these enzyme complexes use a small molecular weight effector protein to modulate the function of the hydroxylase. however, the origin of these functional changes is poorly understood. here, we report the structures of the biologically r ... | 2008 | 19033467 |
| improving biocatalyst performance by integrating statistical methods into protein engineering. | directed evolution and rational design were used to generate active variants of toluene-4-monooxygenase (t4mo) on 2-phenylethanol (pea), with the aim of producing hydroxytyrosol, a potent antioxidant. due to the complexity of the enzymatic system-four proteins encoded by six genes-mutagenesis is labor-intensive and time-consuming. therefore, the statistical model of nov and wein (j. comput. biol. 12:247-282) was used to reduce the number of variants produced and evaluated in a lab. from an initi ... | 2010 | 20709845 |
| evolutionary dynamics of clustered irregularly interspaced short palindromic repeat systems in the ocean metagenome. | clustered regularly interspaced short palindromic repeats (crisprs) form a recently characterized type of prokaryotic antiphage defense system. the phage-host interactions involving crisprs have been studied in experiments with selected bacterial or archaeal species and, computationally, in completely sequenced genomes. however, these studies do not allow one to take prokaryotic population diversity and phage-host interaction dynamics into account. this gap can be filled by using metagenomic dat ... | 2010 | 20118362 |
| induction of drought tolerance in cucumber plants by a consortium of three plant growth-promoting rhizobacterium strains. | our previous work showed that a consortium of three plant growth-promoting rhizobacterium (pgpr) strains (bacillus cereus ar156, bacillus subtilis sm21, and serratia sp. xy21), termed as bbs for short, was a promising biocontrol agent. the present study investigated its effect on drought tolerance in cucumber plants. after withholding watering for 13 days, bbs-treated cucumber plants had much darker green leaves and substantially lighter wilt symptoms than control plants. compared to the control ... | 2012 | 23285089 |
| genome sequence of pseudomonas mendocina dlhk, isolated from a biotrickling reactor. | pseudomonas mendocina dlhk is an aerobic bacterium isolated from a biotrickling reactor which can remove nitric oxide, a common air pollutant from combustion exhaust gas. here, we present the draft genome of pseudomonas mendocina dlhk. | 2012 | 23105066 |
| heavy-metal resistance of a france vineyard soil bacterium, pseudomonas mendocina strain s5.2, revealed by whole-genome sequencing. | here we present the draft genome of pseudomonas mendocina strain s5.2, possessing tolerance to a high concentration of copper. in addition to being copper resistant, the genome of p. mendocina strain s5.2 contains a number of heavy-metal-resistant genes known to confer resistance to multiple heavy-metal ions. | 2012 | 23105092 |
| microbial community structure at different fermentation stages of kutajarista, a herbal formulation. | kutajarista is an ayurvedic fermented herbal formulation prescribed for gastrointestinal disorders. this herbal formulation undergoes a gradual fermentative process and takes around 2 months for production. in this study, microbial composition at initial stages of fermentation of kutajarista was assessed by culture independent 16s rrna gene clone library approach. physicochemical changes were also compared at these stages of fermentation. high performance liquid chromatography-mass spectrometry ... | 2012 | 24426073 |
| microbial community structure at different fermentation stages of kutajarista, a herbal formulation. | kutajarista is an ayurvedic fermented herbal formulation prescribed for gastrointestinal disorders. this herbal formulation undergoes a gradual fermentative process and takes around 2 months for production. in this study, microbial composition at initial stages of fermentation of kutajarista was assessed by culture independent 16s rrna gene clone library approach. physicochemical changes were also compared at these stages of fermentation. high performance liquid chromatography-mass spectrometry ... | 2012 | 24426073 |
| panning of a phage display library against a synthetic capsule for peptide ligands that bind to the native capsule of bacillus anthracis. | bacillus anthracis is the causative agent of anthrax with the ability to not only produce a tripartite toxin, but also an enveloping capsule comprised primarily of γ-d-glutamic acid residues. the purpose of this study was to isolate peptide ligands capable of binding to the native capsule of b. anthracis from a commercial phage display peptide library using a synthetic form of the capsule consisting of 12 γ-d-glutamic acid residues. following four rounds of selection, 80 clones were selected ran ... | 2012 | 23029033 |
| abrolhos bank reef health evaluated by means of water quality, microbial diversity, benthic cover, and fish biomass data. | the health of the coral reefs of the abrolhos bank (southwestern atlantic) was characterized with a holistic approach using measurements of four ecosystem components: (i) inorganic and organic nutrient concentrations, [1] fish biomass, [1] macroalgal and coral cover and (iv) microbial community composition and abundance. the possible benefits of protection from fishing were particularly evaluated by comparing sites with varying levels of protection. two reefs within the well-enforced no-take are ... | 2012 | 22679480 |
| screening and characterization of a novel alkaline lipase from acinetobacter calcoaceticus 1-7 isolated from bohai bay in china for detergent formulation. | a novel alkaline lipase-producing strain 1-7 identified as acinetobacter calcoaceticus was isolated from soil samples collected from bohai bay, china, using an olive oil alkaline plate, which contained olive oil as the sole carbon source. the lipase from strain 1-7 showed the maximum activity at ph 9.0 under 40 °c. one interesting feature of this enzyme is that it exhibits lipase activity over a broad range of temperatures and good stability. it is also stable at a broad range of phs from 4.0 to ... | 2012 | 24031813 |
| lactate utilization is regulated by the fadr-type regulator lldr in pseudomonas aeruginosa. | nad-independent l-lactate dehydrogenase (l-ildh) and nad-independent d-lactate dehydrogenase (d-ildh) activities are induced coordinately by either enantiomer of lactate in pseudomonas strains. inspection of the genomic sequences of different pseudomonas strains revealed that the lldpde operon comprises 3 genes, lldp (encoding a lactate permease), lldd (encoding an l-ildh), and llde (encoding a d-ildh). cotranscription of lldp, lldd, and llde in pseudomonas aeruginosa strain xmg starts with the ... | 2012 | 22408166 |
| comparative genomics reveals adaptation by alteromonas sp. sn2 to marine tidal-flat conditions: cold tolerance and aromatic hydrocarbon metabolism. | alteromonas species are globally distributed copiotrophic bacteria in marine habitats. among these, sea-tidal flats are distinctive: undergoing seasonal temperature and oxygen-tension changes, plus periodic exposure to petroleum hydrocarbons. strain sn2 of the genus alteromonas was isolated from hydrocarbon-contaminated sea-tidal flat sediment and has been shown to metabolize aromatic hydrocarbons there. strain sn2's genomic features were analyzed bioinformatically and compared to those of alter ... | 2012 | 22563400 |
| dissociation of immune responses from pathogen colonization supports pattern recognition in c. elegans. | caenorhabditis elegans has been used for over a decade to characterize signaling cascades controlling innate immune responses. however, what initiates these responses in the worm has remained elusive. to gain a better understanding of the initiating events we delineated genome-wide immune responses to the bacterial pathogen pseudomonas aeruginosa in worms heavily-colonized by the pathogen versus worms visibly not colonized. we found that infection responses in both groups were identical, suggest ... | 2012 | 22514739 |
| involvement of two latex-clearing proteins during rubber degradation and insights into the subsequent degradation pathway revealed by the genome sequence of gordonia polyisoprenivorans strain vh2. | the increasing production of synthetic and natural poly(cis-1,4-isoprene) rubber leads to huge challenges in waste management. only a few bacteria are known to degrade rubber, and little is known about the mechanism of microbial rubber degradation. the genome of gordonia polyisoprenivorans strain vh2, which is one of the most effective rubber-degrading bacteria, was sequenced and annotated to elucidate the degradation pathway and other features of this actinomycete. the genome consists of a circ ... | 2012 | 22327575 |
| characterization of esterase a, a pseudomonas stutzeri a15 autotransporter. | autotransporters are a widespread family of proteins, generally known as virulence factors produced by gram-negative bacteria. in this study, the esterase a (esta) autotransporter of the rice root-colonizing beneficial bacterium pseudomonas stutzeri a15 was characterized. a multiple sequence alignment identified esta as belonging to clade ii of the gdsl esterase family. autologous overexpression allowed the investigation of several features of both autotransporter proteins and gdsl esterases. fi ... | 2012 | 22307303 |
| novel, highly specific n-demethylases enable bacteria to live on caffeine and related purine alkaloids. | the molecular basis for the ability of bacteria to live on caffeine as a sole carbon and nitrogen source is unknown. pseudomonas putida cbb5, which grows on several purine alkaloids, metabolizes caffeine and related methylxanthines via sequential n-demethylation to xanthine. metabolism of caffeine by cbb5 was previously attributed to one broad-specificity methylxanthine n-demethylase composed of two subunits, ndma and ndmb. here, we report that ndma and ndmb are actually two independent rieske n ... | 2012 | 22328667 |
| modulation of malaria infection in anopheles gambiae mosquitoes exposed to natural midgut bacteria. | the development of plasmodium falciparum within the anopheles gambiae mosquito relies on complex vector-parasite interactions, however the resident midgut microbiota also plays an important role in mediating parasite infection. in natural conditions, the mosquito microbial flora is diverse, composed of commensal and symbiotic bacteria. we report here the isolation of culturable midgut bacteria from mosquitoes collected in the field in cameroon and their identification based on the 16s rrna gene ... | 2013 | 24324714 |
| molybdate reduction to molybdenum blue by an antarctic bacterium. | a molybdenum-reducing bacterium from antarctica has been isolated. the bacterium converts sodium molybdate or mo⁶⁺ to molybdenum blue (mo-blue). electron donors such as glucose, sucrose, fructose, and lactose supported molybdate reduction. ammonium sulphate was the best nitrogen source for molybdate reduction. optimal conditions for molybdate reduction were between 30 and 50 mm molybdate, between 15 and 20°c, and initial ph between 6.5 and 7.5. the mo-blue produced had a unique absorption spectr ... | 2013 | 24381945 |
| kinetics of molybdenum reduction to molybdenum blue by bacillus sp. strain a.rzi. | molybdenum is very toxic to agricultural animals. mo-reducing bacterium can be used to immobilize soluble molybdenum to insoluble forms, reducing its toxicity in the process. in this work the isolation of a novel molybdate-reducing gram positive bacterium tentatively identified as bacillus sp. strain a.rzi from a metal-contaminated soil is reported. the cellular reduction of molybdate to molybdenum blue occurred optimally at 4 mm phosphate, using 1% (w/v) glucose, 50 mm molybdate, between 28 and ... | 2013 | 24369531 |
| genome sequence analysis indicates that the model eukaryote nematostella vectensis harbors bacterial consorts. | analysis of the genome sequence of the starlet sea anemone, nematostella vectensis, reveals many genes whose products are phylogenetically closer to proteins encoded by bacteria or bacteriophages than to any metazoan homologs. one explanation for such sequence affinities could be that these genes have been horizontally transferred from bacteria to the nematostella lineage. we show, however, that bacterium-like and phage-like genes sequenced by the n. vectensis genome project tend to cluster on s ... | 2013 | 23995941 |
| genome sequence of a strain of the human pathogenic bacterium pseudomonas alcaligenes that caused bloodstream infection. | pseudomonas alcaligenes, a gram-negative aerobic bacterium, is a rare opportunistic human pathogen. here, we report the whole-genome sequence of p. alcaligenes strain mry13-0052, which was isolated from a bloodstream infection in a medical institution in japan and is resistant to antimicrobial agents, including broad-spectrum cephalosporins and monobactams. | 2013 | 24179116 |
| selectively dispersed isotope labeling for protein structure determination by magic angle spinning nmr. | the power of nuclear magnetic resonance spectroscopy derives from its site-specific access to chemical, structural and dynamic information. however, the corresponding multiplicity of interactions can be difficult to tease apart. complimentary approaches involve spectral editing on the one hand and selective isotope substitution on the other. here we present a new "redox" approach to the latter: acetate is chosen as the sole carbon source for the extreme oxidation numbers of its two carbons. cons ... | 2013 | 23990199 |
| naoh-debittering induces changes in bacterial ecology during table olives fermentation. | limited information is available on the impact of the naoh treatment on table olive fermentations, and for this reason a polyphasic approach has been adopted here to investigate its effect on the fermentation dynamics and bacterial biodiversity. the microbial counts of the main groups involved in the transformation have not shown any differences, apart from a more prompt start of the fermentation when the olives were subjected to the naoh treatment. the data produced by culture-independent analy ... | 2013 | 23935928 |
| the clinical isolate pseudomonas aeruginosa mma83 carries two copies of the blandm-1 gene in a novel genetic context. | the genetic context of the blandm-1 gene in the genome of pseudomonas aeruginosa mma83 was investigated. sequencing of the cosmid selected for the blandm-1 gene revealed the presence of two blandm-1 copies in the genome of p. aeruginosa mma83 in a unique genetic environment. additionally, mating assays, dna-dna hybridization, and an s1 nuclease assay strongly suggest that the blandm-1 gene in p. aeruginosa mma83 is chromosome borne. | 2013 | 23612199 |
| diversity of microbial communities in production and injection waters of algerian oilfields revealed by 16s rrna gene amplicon 454 pyrosequencing. | the microorganisms inhabiting many petroleum reservoirs are multi-extremophiles capable of surviving in environments with high temperature, pressure and salinity. their activity influences oil quality and they are an important reservoir of enzymes of industrial interest. to study these microbial assemblages and to assess any modifications that may be caused by industrial practices, the bacterial and archaeal communities in waters from four algerian oilfields were described and compared. three di ... | 2013 | 23805243 |
| discovery of functional toxin/antitoxin systems in bacteria by shotgun cloning. | toxin-antitoxin (ta) modules, composed of a toxic protein and a counteracting antitoxin, play important roles in bacterial physiology. we examined the experimental insertion of 1.5 million genes from 388 microbial genomes into an escherichia coli host using more than 8.5 million random clones. this revealed hundreds of genes (toxins) that could only be cloned when the neighboring gene (antitoxin) was present on the same clone. clustering of these genes revealed ta families widespread in bacteria ... | 2013 | 23478446 |
| draft genome sequence of chromate-resistant and biofilm-producing strain pseudomonas alcaliphila 34. | we report the draft genome sequence of pseudomonas alcaliphila 34, a cr(vi)-hyperresistant and biofilm-producing bacterium that might be used for the bioremediation of chromate-polluted soils. the genome sequence might be helpful in exploring the mechanisms involved in chromium resistance and biofilm formation. | 2013 | 23469336 |
| ferritin and ferrihydrite nanoparticles as iron sources for pseudomonas aeruginosa. | metabolism of iron derived from insoluble and/or scarce sources is essential for pathogenic and environmental microbes. the ability of pseudomonas aeruginosa to acquire iron from exogenous ferritin was assessed; ferritin is an iron-concentrating and antioxidant protein complex composed of a catalytic protein and caged ferrihydrite nanomineral synthesized from fe(ii) and o(2) or h(2)o(2). ferritin and free ferrihydrite supported growth of p. aeruginosa with indistinguishable kinetics and final cu ... | 2013 | 23417538 |
| simultaneous quantification of multiple bacteria by the bactochip microarray designed to target species-specific marker genes. | bacteria are ubiquitous throughout the environment, the most abundant inhabitants of the healthy human microbiome, and causal pathogens in a variety of diseases. their identification in disease is often an essential step in rapid diagnosis and targeted intervention, particularly in clinical settings. at present, clinical bacterial detection and discrimination is primarily culture-based, requiring both time and microbiological expertise, especially for bacteria that are not easily cultivated. hig ... | 2013 | 23409037 |
| adsorption studies of the gram-negative bacteria onto nanostructured silicon carbide. | in this study, we demonstrated a significant adsorption of pseudomonas putida bacteria onto aggregates of nanofibers (nfsic) and nanorods (nrsic) of silicon carbide (sic) in aqueous suspensions. langmuir and freundlich isotherms were used to quantify adsorption affinities. it was found that adsorption of the bacteria strongly depended on the structure of the silicon carbide and the ph of the aqueous solution, which affected the isoelectric point of both the silicon carbide and the bacterial cell ... | 2014 | 25410802 |
| adsorption studies of the gram-negative bacteria onto nanostructured silicon carbide. | in this study, we demonstrated a significant adsorption of pseudomonas putida bacteria onto aggregates of nanofibers (nfsic) and nanorods (nrsic) of silicon carbide (sic) in aqueous suspensions. langmuir and freundlich isotherms were used to quantify adsorption affinities. it was found that adsorption of the bacteria strongly depended on the structure of the silicon carbide and the ph of the aqueous solution, which affected the isoelectric point of both the silicon carbide and the bacterial cell ... | 2014 | 25410802 |
| monitoring of the environment at the transplant unit-hemato-oncology clinic. | aim of this study was to monitor the environment at the transplant unit-hemato-oncology clinic, university hospital olomouc (olomouc, czech republic) and identify risks for the patients. | 2014 | 25222472 |
| growth kinetics, effect of carbon substrate in biosynthesis of mcl-pha by pseudomonas putida bet001. | growth associated biosynthesis of medium chain length poly-3-hydroxyalkanoates (mcl-pha) in pseudomonas putida bet001 isolated from palm oil mill effluent was studied. models with substrate inhibition terms described well the kinetics of its growth. selected fatty acids (c8:0 to c18:1) and ammonium were used as carbon and nitrogen sources during growth and pha biosynthesis, resulting in pha accumulation of about 50 to 69% (w/w) and pha yields ranging from 10.12 g l(-1) to 15.45 g l(-1), respecti ... | 2014 | 25242925 |
| (s)-3-hydroxyacyl-coa dehydrogenase/enoyl-coa hydratase (fadb') from fatty acid degradation operon of ralstonia eutropha h16. | in this study (s)-3-hydroxyacyl-coa dehydrogenase/enoyl-coa hydratase (h16_a0461/fadb', gene id: 4247876) from one of two active fatty acid degradation operons of ralstonia eutropha h16 has been heterologously expressed in escherichia coli, purified as protein possessing a his-tag and initially characterized. fadb' is an enzyme with two catalytic domains exhibiting a single monomeric structure and possessing a molecular weight of 86 kda. the c-terminal part of the enzyme harbors enoyl-coa hydrat ... | 2014 | 25401070 |
| impact of next generation sequencing techniques in food microbiology. | understanding the maxam-gilbert and sanger sequencing as the first generation, in recent years there has been an explosion of newly-developed sequencing strategies, which are usually referred to as next generation sequencing (ngs) techniques. ngs techniques have high-throughputs and produce thousands or even millions of sequences at the same time. these sequences allow for the accurate identification of microbial taxa, including uncultivable organisms and those present in small numbers. in speci ... | 2014 | 25132799 |
| multiple signals modulate the activity of the complex sensor kinase tods. | the reason for the existence of complex sensor kinases is little understood but thought to lie in the capacity to respond to multiple signals. the complex, seven-domain sensor kinase tods controls in concert with the todt response regulator the expression of the toluene dioxygenase pathway in pseudomonas putida f1 and dot-t1e. we have previously shown that some aromatic hydrocarbons stimulate tods activity whereas others behave as antagonists. we show here that tods responds in addition to the o ... | 2014 | 24986263 |
| multiple signals modulate the activity of the complex sensor kinase tods. | the reason for the existence of complex sensor kinases is little understood but thought to lie in the capacity to respond to multiple signals. the complex, seven-domain sensor kinase tods controls in concert with the todt response regulator the expression of the toluene dioxygenase pathway in pseudomonas putida f1 and dot-t1e. we have previously shown that some aromatic hydrocarbons stimulate tods activity whereas others behave as antagonists. we show here that tods responds in addition to the o ... | 2014 | 24986263 |
| thermodynamic and structural investigation of the specific sds binding of humicola insolens cutinase. | the interaction of lipolytic enzymes with anionic surfactants is of great interest with respect to industrially produced detergents. here, we report the interaction of cutinase from the thermophilic fungus humicola insolens with the anionic surfactant sds, and show the enzyme specifically binds a single sds molecule under nondenaturing concentrations. protein interaction with sds was investigated by nmr, itc and molecular dynamics simulations. the nmr resonances of the protein were assigned, wit ... | 2014 | 24832484 |
| secretome analysis reveals effector candidates associated with broad host range necrotrophy in the fungal plant pathogen sclerotinia sclerotiorum. | the white mold fungus sclerotinia sclerotiorum is a devastating necrotrophic plant pathogen with a remarkably broad host range. the interaction of necrotrophs with their hosts is more complex than initially thought, and still poorly understood. | 2014 | 24886033 |
| ecobiotechnological strategy to enhance efficiency of bioconversion of wastes into hydrogen and methane. | vegetable wastes (vw) and food wastes (fw) are generated in large quantities by municipal markets, restaurants and hotels. waste slurries (250 ml) in 300 ml bod bottles, containing 3, 5 and 7 % total solids (ts) were hydrolyzed with bacterial mixtures composed of: bacillus, acinetobacter, exiguobacterium, pseudomonas, stenotrophomonas and sphingobacterium species. each of these bacteria had high activities for the hydrolytic enzymes: amylase, protease and lipase. hydrolysate of biowaste slurries ... | 2014 | 24891732 |
| an airborne actinobacteria nocardiopsis alba isolated from bioaerosol of a mushroom compost facility. | actinobacteria are widely distributed in many environments and represent the most important trigger to the occupant respiratory health. health complaints, including hypersensitivity pneumonitis of the workers, were recorded in a mushroom compost facility (mcf). the studies on the airborne bacteria were carried out to find a possible microbiological source of these symptoms. culture analysis of compost bioaerosols collected in different location of the mcf was performed. an assessment of the indo ... | 2014 | 25382928 |
| abattoirs as non-hospital source of extended spectrum beta lactamase producers: confirmed by the double disc synergy test and characterized by matrix-assisted laser desorption/ionization time of flight mass spectrometry. | in this study, the presence of extended spectrum beta lactamase (esbl) producing organisms in abattoirs, a non-hospital community was investigated. the presence of esbl-producing phenotypes was confirmed by the double disc synergy test (ddst). out of the 99 isolates screened for esbl, 28 (28.3%) were confirmed positive. the positive isolates were characterised by using matrix-assisted laser desorption/ionization time of flight mass spectrometry. 50% of the isolates were pseudomonas spp., the res ... | 2014 | 24728403 |
| from structure to catalysis: recent developments in the biotechnological applications of lipases. | microbial lipases are highly appreciated as biocatalysts due to their peculiar characteristics such as the ability to utilize a wide range of substrates, high activity and stability in organic solvents, and regio- and/or enantioselectivity. these enzymes are currently being applied in a variety of biotechnological processes, including detergent preparation, cosmetics and paper production, food processing, biodiesel and biopolymer synthesis, and the biocatalytic resolution of pharmaceutical deriv ... | 2014 | 24783219 |
| emergence of citrobacter freundii carrying imp-8 metallo-β-lactamase in germany. | metallo-β-lactamases (mbls) in enterobacteriaceae are an increasing problem worldwide. this report describes the isolation of citrobacter freundii carrying imp-8 mbl from three patients during the period from march 2012 until march 2013 in germany. the bla imp-8 enzyme is predominantly found in asia, where imp-8 has spread to various enterobacterial species causing serious infections. to our best knowledge, this is the first report of bla imp-8 habouring enterobacteriaceae in europe. | 2014 | 25356340 |
| t cell epitope mimicry between sjögren's syndrome antigen a (ssa)/ro60 and oral, gut, skin and vaginal bacteria. | this study was undertaken to test the hypothesis that sjogren's syndrome antigen a (ssa)/ro60-reactive t cells are activated by peptides originating from oral and gut bacteria. t cell hybridomas generated from hla-dr3 transgenic mice recognized 3 regions on ro60, with core epitopes mapped to amino acids 228-238, 246-256 and 371-381. blast analysis identified several mimicry peptides, originating from human oral, intestinal, skin and vaginal bacteria, as well as environmental bacteria. amongst th ... | 2014 | 24576620 |
| microbial ecology of anaerobic digesters: the key players of anaerobiosis. | anaerobic digestion is the method of wastes treatment aimed at a reduction of their hazardous effects on the biosphere. the mutualistic behavior of various anaerobic microorganisms results in the decomposition of complex organic substances into simple, chemically stabilized compounds, mainly methane and co2. the conversions of complex organic compounds to ch4 and co2 are possible due to the cooperation of four different groups of microorganisms, that is, fermentative, syntrophic, acetogenic, and ... | 2014 | 24701142 |
| diagnosis of prosthetic joint infection by use of pcr-electrospray ionization mass spectrometry. | we compared pcr-electrospray ionization mass spectrometry (pcr-esi/ms) to culture using sonicate fluid from 431 subjects with explanted knee (n = 270) or hip (n = 161) prostheses. of these, 152 and 279 subjects had prosthetic joint infection (pji) and aseptic failure, respectively. the sensitivities for detecting pji were 77.6% for pcr-esi/ms and 69.7% for culture (p = 0.0105). the specificities were 93.5 and 99.3%, respectively (p = 0.0002). | 2014 | 24478503 |
| draft genome sequence of petroleum oil-degrading marine bacterium pseudomonas taeanensis strain ms-3, isolated from a crude oil-contaminated seashore. | pseudomonas taeanensis ms-3(t), isolated from a crude oil-contaminated seashore in south korea, is capable of degrading petroleum oils, such as gasoline, diesel, and kerosene. here, we report the draft genome sequence of this strain, which consists of 5,477,045 bp, with a g+c content of 60.72%. | 2014 | 24407626 |
| escherichia coli with bla(imp-8) in singapore. | | 2014 | 24145544 |
| an antimicrobial metabolite from bacillus sp.: significant activity against pathogenic bacteria including multidrug-resistant clinical strains. | in this study, the cell free modified tryptone soya broth (ph 7.4 ± 0.2) of bacillus subtilis urid 12.1 showed significant antimicrobial activity against multidrug-resistant strains of staphylococcus aureus, s. epidermidis, streptococcus pyogenes and enterococcus faecalis. the partially purified antimicrobial molecule was found to be resistant to extremes of ph and temperatures and also to higher concentrations of trypsin and proteinase k. the antimicrobial molecule was purified by a three-step ... | 2015 | 26696963 |
| the yhs-domain of an adenylyl cyclase from mycobacterium phlei is a probable copper-sensor module. | yhs-domains are small protein modules which have been proposed to bind transition-metal ions like the related trash-domains. they are found in a variety of enzymes including copper-transporting atpases and adenylyl cyclases. here we investigate a class iiic adenylyl cyclase from mycobacterium phlei which contains a c-terminal yhs-domain linked to the catalytic domain by a peptide of 8 amino acids. we expressed the isolated catalytic domain and the full-length enzyme in e. coli. the catalytic dom ... | 2015 | 26512893 |
| community of thermoacidophilic and arsenic resistant microorganisms isolated from a deep profile of mine heaps. | soluble arsenic (as) in acidic feed solution may inhibit the copper (cu) bioleaching process within mine heaps. to clarify the effect of soluble arsenic on the live biomass and bioxidative activity in heaps, toxicological assays were performed using a synthetic feed solution given by a mine company. the microorganisms had previously been isolated from two heap samples at up to 66 m depth, and cultured using specific media for chemolithotrophic acidophiles (ph 1-2) and moderate thermophiles (48°c ... | 2015 | 26283066 |
| genomic analysis reveals the biotechnological and industrial potential of levan producing halophilic extremophile, halomonas smyrnensis aad6t. | halomonas smyrnensis aad6t is a gram negative, aerobic, and moderately halophilic bacterium, and is known to produce high levels of levan with many potential uses in foods, feeds, cosmetics, pharmaceutical and chemical industries due to its outstanding properties. here, the whole-genome analysis was performed to gain more insight about the biological mechanisms, and the whole-genome organization of the bacterium. industrially crucial genes, including the levansucrase, were detected and the genom ... | 2015 | 26251777 |
| characterization of pkp-m1144, a novel cole1-like plasmid encoding imp-8, ges-5, and bel-1 β-lactamases, from a klebsiella pneumoniae sequence type 252 isolate. | imp-8 metallo-β-lactamase was identified in klebsiella pneumoniae sequence type 252 (st252), isolated in a portuguese hospital in 2009. blaimp-8 was the first gene cassette of a novel class 3 integron, in1144, also carrying the blages-5, blabel-1, and aaca4 cassettes. in1144 was located on a cole1-like plasmid, pkp-m1144 (12,029 bp), with a replication region of limited nucleotide similarity to those of other rna-priming plasmids, such as pjhcmw1. in1144 and pkp-m1144 represent an interesting ca ... | 2015 | 26033721 |
| efflux pump-deficient mutants as a platform to search for microbes that produce antibiotics. | pseudomonas putida dot-t1e-18 is a strain deficient in the major antibiotic efflux pump (ttgabc) that exhibits an overall increased susceptibility to a wide range of drugs when compared with the wild-type strain. we used this strain as a platform to search for microbes able to produce antibiotics that inhibit growth. a collection of 2400 isolates from soil, sediments and water was generated and a drop assay developed to identify, via growth inhibition halos, strains that prevent the growth of do ... | 2015 | 26059350 |
| microbial community in high arsenic shallow groundwater aquifers in hetao basin of inner mongolia, china. | a survey was carried out on the microbial community of 20 groundwater samples (4 low and 16 high arsenic groundwater) and 19 sediments from three boreholes (two high arsenic and one low arsenic boreholes) in a high arsenic groundwater system located in hetao basin, inner mongolia, using the 454 pyrosequencing approach. a total of 233,704 sequence reads were obtained and classified into 12-267 operational taxonomic units (otus). groundwater and sediment samples were divided into low and high arse ... | 2015 | 25970606 |
| approaches to studying and manipulating the enteric microbiome to improve autism symptoms. | there is a growing body of scientific evidence that the health of the microbiome (the trillions of microbes that inhabit the human host) plays an important role in maintaining the health of the host and that disruptions in the microbiome may play a role in certain disease processes. an increasing number of research studies have provided evidence that the composition of the gut (enteric) microbiome (gm) in at least a subset of individuals with autism spectrum disorder (asd) deviates from what is ... | 2015 | 25956237 |
| recovery of critical metals using biometallurgy. | the increased development of green low-carbon energy technologies that require platinum group metals (pgms) and rare earth elements (rees), together with the geopolitical challenges to sourcing these metals, has spawned major governmental and industrial efforts to rectify current supply insecurities. as a result of the increasing critical importance of pgms and rees, environmentally sustainable approaches to recover these metals from primary ores and secondary streams are needed. in this review, ... | 2015 | 25912797 |
| pan-genome dynamics of pseudomonas gene complements enriched across hexachlorocyclohexane dumpsite. | phylogenetic heterogeneity across pseudomonas genus is complemented by its diverse genome architecture enriched by accessory genetic elements (plasmids, transposons, and integrons) conferring resistance across this genus. here, we sequenced a stress tolerant genotype i.e. pseudomonas sp. strain rl isolated from a hexachlorocyclohexane (hch) contaminated pond (45 mg of total hch g(-1) sediment) and further compared its gene repertoire with 17 reference ecotypes belonging to p. stutzeri, p. mendoc ... | 2015 | 25898829 |
| nitrogen removal from micro-polluted reservoir water by indigenous aerobic denitrifiers. | treatment of micro-polluted source water is receiving increasing attention because of environmental awareness on a global level. we isolated and identified aerobic denitrifying bacteria zoogloea sp. n299, acinetobacter sp. g107, and acinetobacter sp. 81y and used these to remediate samples of their native source water. we first domesticated the isolated strains in the source water, and the 48-h nitrate removal rates of strains n299, g107, and 81y reached 33.69%, 28.28%, and 22.86%, respectively, ... | 2015 | 25867475 |
| draft genome sequence of pseudomonas toyotomiensis kf710, a polychlorinated biphenyl-degrading bacterium isolated from biphenyl-contaminated soil. | pseudomonas toyotomiensis kf710 utilizes biphenyl and degrades polychlorinated biphenyls (pcbs). here, we report the genome sequence of the kf710 strain, consisting of 5,596,721 bp with 5,155 coding sequences. the biphenyl catabolic genes were almost identical to those of pseudomonas pseudoalcaligenes kf707, one of the most well-characterized biphenyl-utilizing strains. | 2015 | 25838482 |
| diversity and role of plasmids in adaptation of bacteria inhabiting the lubin copper mine in poland, an environment rich in heavy metals. | the lubin underground mine, is one of three mining divisions in the lubin-glogow copper district in lower silesia province (poland). it is the source of polymetallic ore that is rich in copper, silver and several heavy metals. black shale is also significantly enriched in fossil organic matter in the form of long-chain hydrocarbons, polycyclic aromatic hydrocarbons, organic acids, esters, thiophenes and metalloporphyrins. biological analyses have revealed that this environment is inhabited by ex ... | 2015 | 26074880 |
| malpighian tubules are important determinants of pseudomonas transstadial transmission and longtime persistence in anopheles stephensi. | pseudomonas is a genus of bacteria commonly found in investigations of gut microbes in malaria mosquitoes. among those mosquitoes is the dominating malaria vector in asia, anopheles stephensi, where pseudomonas is a prevailing bacterium and natural inhabitant of its breeding places. in order to explore the reason for finding pseudomonas so frequently, an investigation of its localization and transstadial properties was undertaken. | 2015 | 25604581 |
| diagnostic value of bronchoalveolar lavage in leukemic and bone marrow transplant patients: the impact of antimicrobial therapy. | there is significant morbidity and mortality from pneumonia in leukemic and bone marrow transplant patients. we sought to explore the diagnostic yield of bronchoalveolar lavage (bal) in these patients with new pulmonary infiltrates. a retrospective chart review of approximately 200 non- human immunodeficiency virus (hiv) leukemic and hematopoietic stem cell transplantation (hsct) patients who underwent bronchoscopy at a single academic cancer center was performed. antimicrobial use for less than ... | 2015 | 25574361 |
| the diguanylate cyclase hsbd intersects with the hptb regulatory cascade to control pseudomonas aeruginosa biofilm and motility. | the molecular basis of second messenger signaling relies on an array of proteins that synthesize, degrade or bind the molecule to produce coherent functional outputs. cyclic di-gmp (c-di-gmp) has emerged as a eubacterial nucleotide second messenger regulating a plethora of key behaviors, like the transition from planktonic cells to biofilm communities. the striking multiplicity of c-di-gmp control modules and regulated cellular functions raised the question of signaling specificity. are c-di-gmp ... | 2016 | 27792789 |
| host-specific functional significance of caenorhabditis gut commensals. | the gut microbiota is an important contributor to host health and fitness. given its importance, microbiota composition should not be left to chance. however, what determines this composition is far from clear, with results supporting contributions of both environmental factors and host genetics. to gauge the relative contributions of host genetics and environment, specifically the microbial diversity, we characterized the gut microbiotas of caenorhabditis species spanning 200-300 million years ... | 2016 | 27799924 |
| pseudomonas mendocina native valve infective endocarditis: a case report. | gram-negative microorganisms are uncommon pathogens responsible for infective endocarditis. pseudomonas mendocina, a gram-negative water-borne and soil-borne bacterium, was first reported to cause human infection in 1992. since then, it has rarely been reported as a human pathogen in the literature. we describe the first case of native valve infective endocarditis due to p. mendocina in the usa. | 2016 | 27716406 |
| biodegradation of methyl tert-butyl ether by co-metabolism with a pseudomonas sp. strain. | co-metabolic bioremediation is supposed to be an impressive and promising approach in the elimination technology of methyl tert-butyl ether (mtbe), which was found to be a common pollutant worldwide in the ground or underground water in recent years. in this paper, bacterial strain dz13 (which can co-metabolically degrade mtbe) was isolated and named as pseudomonas sp. dz13 based on the result of 16s rrna gene sequencing analysis. strain dz13 could grow on n-alkanes (c₅-c₈), accompanied with the ... | 2016 | 27608032 |
| the native microbiome of the nematode caenorhabditis elegans: gateway to a new host-microbiome model. | host-microbe associations underlie many key processes of host development, immunity, and life history. yet, none of the current research on the central model species caenorhabditis elegans considers the worm's natural microbiome. instead, almost all laboratories exclusively use the canonical strain n2 and derived mutants, maintained through routine bleach sterilization in monoxenic cultures with an e. coli strain as food. here, we characterize for the first time the native microbiome of c. elega ... | 2016 | 27160191 |
| siderophore-mediated iron dissolution from nontronites is controlled by mineral cristallochemistry. | bacteria living in oxic environments experience iron deficiency due to limited solubility and slow dissolution kinetics of iron-bearing minerals. to cope with iron deprivation, aerobic bacteria have evolved various strategies, including release of siderophores or other organic acids that scavenge external fe(iii) and deliver it to the cells. this research investigated the role of siderophores produced by pseudomonas aeruginosa in the acquisition of fe(iii) from two iron-bearing colloidal nontron ... | 2016 | 27064911 |
| direct identification of urinary tract pathogens from urine samples, combining urine screening methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry. | early diagnosis of urinary tract infections (utis) is essential to avoid inadequate or unnecessary empirical antibiotic therapy. microbiological confirmation takes 24 to 48 h. the use of screening methods, such as cytometry and automated microscopic analysis of urine sediment, allows the rapid prediction of negative samples. in addition, matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) is a widely established technique in clinical microbiology laborator ... | 2016 | 26818668 |
| molecular insights into toluene sensing in the tods/todt signal transduction system. | tods is a sensor kinase that responds to various monoaromatic compounds, which either cause an agonistic or antagonistic effect on phosphorylation of its cognate response regulator todt, and controls tod operon expression in pseudomonas putida strains. we describe a molecular sensing mechanism of tods that is activated in response to toluene. the crystal structures of the tods per-arnt-sim (pas) 1 sensor domain (residues 43-164) and its complex with toluene (agonist) or 1,2,4-trimethylbenzene (a ... | 2016 | 26903514 |
| protein sequence annotation tool (psat): a centralized web-based meta-server for high-throughput sequence annotations. | here we introduce the protein sequence annotation tool (psat), a web-based, sequence annotation meta-server for performing integrated, high-throughput, genome-wide sequence analyses. our goals in building psat were to (1) create an extensible platform for integration of multiple sequence-based bioinformatics tools, (2) enable functional annotations and enzyme predictions over large input protein fasta data sets, and (3) provide a web interface for convenient execution of the tools. | 2016 | 26792120 |
| all the microbiology nematodes can teach us. | be it their pervasiveness, experimental tractability or their impact on human health and agriculture, nematode-bacterium associations are far-reaching research subjects. although the omics hype did not spare them and helped reveal mechanisms of communication and exchange between the associated partners, a huge amount of knowledge still awaits to be harvested from their study. here, i summarize and compare the kind of research that has been already performed on the model nematode caenorhabditis e ... | 2016 | 26839382 |
| a case of unusual gram-negative bacilli septic arthritis in an immunocompetent patient. | the gram-negative bacilli acinetobacter baumannii, burkholderia cepacia, ochrobactrum anthropi, pseudomonas mendocina, ralstonia spp., serratia marcescens and stenotrophomonas maltophilia are ubiquitous environmental organisms of low virulence, and do not usually cause illness in immunocompetent hosts. we report a case of multiple concurrent opportunistic gram-negative bacilli causing septic arthritis in a healthy patient following trauma to the knee. repeated operations, including arthroscopy, ... | 2013 | 24005465 |
| bacterial community analysis in chlorpyrifos enrichment cultures via dgge and use of bacterial consortium for cp biodegradation. | the organophosphate pesticide chlorpyrifos (cp) has been used extensively since the 1960s for insect control. however, its toxic effects on mammals and persistence in environment necessitate its removal from contaminated sites, biodegradation studies of cp-degrading microbes are therefore of immense importance. samples from a pakistani agricultural soil with an extensive history of cp application were used to prepare enrichment cultures using cp as sole carbon source for bacterial community anal ... | 2014 | 25008559 |
| milk microbiome signatures of subclinical mastitis-affected cattle analysed by shotgun sequencing. | metagenomic analysis of milk samples collected from kankrej, gir (bos indicus) and crossbred (bos taurus × b. indicus) cattle harbouring subclinical mastitis was carried out by next-generation sequencing 454 gs-flx technology to elucidate the microbial community structure of cattle milk. | 2012 | 22277077 |
| ligational behavior of schiff bases towards transition metal ion and metalation effect on their antibacterial activity. | new schiff bases pyrazine-2-carboxylicacid (phenyl-pyridin-2-yl-methylene)-hydrazide (hpch-bp) hl(1) and pyrazine-2-carboxylicacid (pyridin-2-ylmethylene)-hydrazide (hpch-pc) hl(2) derived from condensation of pyrazine carboxylic hydrazide (hpch) with 2-benzoyl pyridine (bp) or pyridine 2-carbaldehyde (pc) and their transition metal complexes of type ml((1-2)2) have been synthesized, where m=mn(ii), co(ii), ni(ii), cu(ii) and zn(ii). characterization of ligands and their metal complexes was carr ... | 2012 | 22813991 |
| t4 lysozyme fused with cellulose-binding module for antimicrobial cellulosic wound dressing materials. | bacterial infection is a major challenge in wound care. antimicrobial wound dressings are of great value for treating wound infections. endolysins are evolving as a new class of antimicrobials with multiple applications. this study describes the production and evaluation of t4 lysozyme (t4lyz), product of gene e of the t4 bacteriophage, fused with cellulose binding module (cbm) for facile immobilization to cellulosic wound dressing. | 2016 | 27028513 |
| saturation mutagenesis of bradyrhizobium sp. btai1 toluene 4-monooxygenase at alpha-subunit residues proline 101, proline 103, and histidine 214 for regiospecific oxidation of aromatics. | a novel toluene monooxygenase (tmo) six-gene cluster from bradyrhizobium sp. btai1 having an overall 35, 36, and 38 % protein similarity with toluene o-xylene monooxygenase (tomo) of pseudomonas sp. ox1, toluene 4-monooxygenase (t4mo) of pseudomonas mendocina kr1, and toluene-para-monooxygenase (tpmo) of ralstonia pickettii pko1, respectively, was cloned and expressed in escherichia coli tg1, and its potential activity was investigated for aromatic hydroxylation and trichloroethylene (tce) degra ... | 2014 | 25016343 |
| photoassisted biodegradation of irradiated organics in simulated nuclear wastewater. | the extent of dehalogenation and degradation of toxic aromatic compounds in a nuclear wastewater was evaluated using a two-stage system consisting of a photolytic reactor followed by a biological reactor. experiments were performed by varying the initial 4-chlorophenol (4-cp) concentration from 50 to 1000 mg/l. the uv pretreatment stage improved the overall efficiency of biodegradation of the recalcitrant compound by facilitating degradability in the biological stage. removal efficiencies greate ... | 2015 | 26460459 |
| oxidation of gasoline oxygenates by closely related non-haem-iron alkane hydroxylases in pseudomonas mendocina kr1 and other n-octane-utilizing pseudomonas strains. | pseudomonas mendocina kr1 oxidizes the gasoline oxygenate methyl tertiary butyl ether (mtbe) to tertiary butyl alcohol (tba) during growth on c5 -c8 n-alkanes. we have further explored oxidation of ether oxygenates by this strain to help identify the enzyme that catalyses these reactions. high levels of mtbe-oxidizing activity occurred in resting cells grown on c5 -c8 n-alkanes. lower activities occurred in cells grown on longer-chain n-alkanes (c9 -c11 ) and 1°-alcohols (c5 -c10 ). n-octane-gro ... | 2010 | 23766116 |
| pseudomonas guguanensis sp. nov., a gammaproteobacterium isolated from a hot spring. | an aerobic, gram-stain-negative, rod-shaped bacterium (designated strain cc-g9a(t)), motile by a polar-flagellum, was isolated from a hot spring water sample in taiwan. strain cc-g9a(t) could grow at 20-42 °c, ph 6.0-10.0 and tolerate up to 7% (w/v) nacl. the 16s rrna gene sequence analysis of strain cc-g9a(t) showed pairwise sequence similarity to pseudomonas mendocina lmg 1223(t) (97.7%), pseudomonas alcaligenes atcc 14909(t) (97.8 %), pseudomonas alcaliphila dsm 17744(t) (97.8 %), pseudomonas ... | 2013 | 23918786 |
| characterization of the ability to form biofilms by plant-associated pseudomonas species. | successful colonization is the initial step for plant-bacteria interactions; therefore, the development of strategies to improve adherence to plant surfaces is critically important for environmental bacteria. biofilm formation is thought to be one such strategy for bacteria to establish stable colonization on inert and living surfaces. although biofilms play potential roles in enabling persistent bacterial colonization, little attention has been paid to biofilms formed by plant-associated bacter ... | 2015 | 25487118 |
| comparative investigation on a hexane-degrading strain with different cell surface hydrophobicities mediated by starch and chitosan. | bioremediation usually exhibits low removal efficiency toward hexane because of poor water solubility, which limits the mass transfer rate between the substrate and microorganism. this work aimed to enhance the hexane degradation rate by increasing cell surface hydrophobicity (csh) of the degrader, pseudomonas mendocina nx-1. the csh of p. mendocina nx-1 was manipulated by treatment with starch and chitosan solution of varied concentrations, reaching a maximum hydrophobicity of 52%. the biodegra ... | 2017 | 28091789 |