| cloning and expression of the gene for periplasmic poly(vinyl alcohol) dehydrogenase from sphingomonas sp. strain 113p3, a novel-type quinohaemoprotein alcohol dehydrogenase. | a gene for periplasmic poly(vinyl alcohol) (pva) dehydrogenase (pvadh) was cloned, based on the n-terminal amino acid sequence of the purified pvadh from sphingomonas sp. 113p3 and the sequence of the gene for pvadh (pvaa, genbank accession no. ab190288). the recombinant pvadh tagged with hexahistidine was expressed in escherichia coli and purified to homogeneity. the recombinant enzyme had the same characteristics as the purified enzyme from sphingomonas sp. strain 113p. in addition to pva, the ... | 2006 | 16804170 |
| method for assessment of viability and morphological changes of bacteria in the early stage of colony formation on a simulated natural environment. | a quantitative analysis of changes in the physiological status of bacterial cells is a fundamental type of study in microbiological research. we devised a method for measuring the viability of bacteria in the early stage of colony formation on a simulated natural environment. in this method, a solid medium containing soil extract was used, and the formation of bacterial microcolonies on a membrane filter was determined by use of a laser scanning cytometer combined with live-dead fluorescent dyes ... | 2006 | 16820503 |
| evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods. | to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ... | 2007 | 16822636 |
| molecular detection and diversity of medium-chain-length polyhydroxyalkanoates-producing bacteria enriched from activated sludge. | knowledge of the species composition of complex bacterial communities is still very limited. the main objectives of this study were to identify medium-chain-length polyhydroxyalkanoates (mcl-phas)-producing bacteria from activated sludge fed with methanol as well as to characterize their pha operon. | 2006 | 16834606 |
| structure and activity of bacterial community inhabiting rice roots and the rhizosphere. | root-derived carbon provides a major source for microbial production and emission of ch4 from rice field soils. therefore, we characterized the structure and activity of the bacterial community inhabiting rice roots and the rhizosphere. in the first experiment, dna retrieved from rice roots was analysed for bacterial 16s rrna genes using cloning, sequencing and in situ hybridization. in the second experiment, rice plants were pulse-labelled with 13co2 (99% of atom 13c) for 7 days, and the bacter ... | 2006 | 16872399 |
| bioremediation of soil contaminated with pentachlorophenol (pcp) using humic acids bound on zeolite. | we determined the toxicity of various chlorophenols, especially pentachlorophenol (pcp), on five bacterial strains and studied pcp biodegradation in soils amended with an organomineral complex (omc) prepared from humic acids (organic part) bound on zeolite (inorganic part). both components of omc have excellent sorption properties and are of natural origin and therefore suitable to be used in the environment. toxicity of chlorophenols depends not only on the number of chlorine atoms but also on ... | 2007 | 16876229 |
| orf18-disrupted mutant of comamonas testosteroni ta441 accumulates significant amounts of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid and its derivatives after incubation with steroids. | in a steroid degradation gene cluster of comamonas testosteroni ta441 consisting of orf18, 17 and tesiha2a1defg, orf18 was implicated in encoding a coa-transferase by database searches, but the matching substrate was not clear. in this study, orf18 was shown to be necessary for conversion of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid, a product of hydrolysis of 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-dien-4-oic acid in steroid degradation by ta441. the orf18-disrupted m ... | 2006 | 16891113 |
| biosynthesis of medium chain length poly(3-hydroxyalkanoates) (mcl-phas) by comamonas testosteroni during cultivation on vegetable oils. | comamonas testosteroni has been studied for its ability to synthesize and accumulate medium chain length poly(3-hydroxyalkanoates) (mcl-phas) during cultivation on vegetable oils available in the local market. castor seed oil, coconut oil, mustard oil, cotton seed oil, groundnut oil, olive oil and sesame oil were supplemented in the mineral medium as a sole source of carbon for growth and phas accumulation. the composition of phas was analysed by a coupled gas chromatography/mass spectroscopy (g ... | 2005 | 16084364 |
| production of catalases by comamonas spp. and resistance to oxidative stress. | bacterial isolates comamonas terrigena n3h (from soil contaminated with crude oil) and c. testosteroni (isolated from the sludge of a wastewater treatment plant), exhibit much higher total catalase activity than the same species from laboratory collection cultures. electrophoretic resolution of catalases revealed only one corresponding band in cell-free extracts of both c. testosteroni cultures. isolates of c. terrigena n3h exhibited catalase-1 and catalase-2 activity, whereas in the collection ... | 2005 | 16110914 |
| accessing tetrahydrofuran-based natural products by microbial baeyer-villiger biooxidation. | a heterobicyclic lactone obtained by stereoselective baeyer-villiger biooxidation with recombinant whole-cells expressing cyclopentanone monooxygenase from comamonas sp. ncimb 9872 was used for formal total syntheses of various natural products containing a tetrahydrofuran structural motif. | 2006 | 17028747 |
| impact of membrane fatty acid composition on the uncoupling sensitivity of the energy conservation of comamonas testosteroni atcc 17454. | the fatty acid composition of pyruvate-grown comamonas testosteroni atcc 17454 was analyzed after growth at 30 and 20 degrees c and after half-maximum growth inhibition caused by different membrane-active chemicals at 30 degrees c. palmitic acid (16:0), palmitoleic acid (16:1 omega7c) and vaccenic acid (18:1 omega7c) were the dominant fatty acids. at 20 degrees c, the proportion of palmitic acid decreased and those of palmitoleic and vaccenic acid increased. saturation degree was also lowered wh ... | 2006 | 16133339 |
| active site residues controlling substrate specificity in 2-nitrotoluene dioxygenase from acidovorax sp. strain js42. | acidovorax (formerly pseudomonas) sp. strain js42 utilizes 2-nitrotoluene as sole carbon, nitrogen, and energy source. 2-nitrotoluene 2,3-dioxygenase (2ntdo) catalyzes the initial step in 2-nitrotoluene degradation by converting 2-nitrotoluene to 3-methylcatechol. in this study, we identified specific amino acids at the active site that control specificity. the residue at position 350 was found to be critical in determining both the enantiospecificity of 2ntdo with naphthalene and the ability to ... | 2005 | 16175409 |
| the copy-number of plasmids and other genetic elements can be determined by sybr-green-based quantitative real-time pcr. | in this study, we explored whether sybr green-based quantitative real-time pcr (qpcr) could be used to determine the copy number of a plasmid and whether the method was broadly applicable to chromosomally encoded genetic elements often occurring in multiple copies, such as rrna genes and insertion sequences (is). three different template sources (whole cells, total dna, and restriction-enzyme digested total dna) derived from the bacterium comamonas sp. strain js46 were analyzed by qpcr using pri ... | 2006 | 16216354 |
| enhanced phenol biodegradation and aerobic granulation by two coaggregating bacterial strains. | the effect of coaggregation of the two bacterial strains propioniferax-like pg-02 and comamonassp. pg-08 on phenol degradation and aerobic granulation was investigated. while pg-02 was characterized as a phenol-degrader with a low half-saturation kinetics constant, pg-08 possessed strong aggregation ability with poor phenol degradation ability. the two strains coaggregated through involvement of lectin-saccharide interactions with the adhesin protein on strain pg-02 and the complementary sugar r ... | 2006 | 17051812 |
| characterization of floating activity of indigenous diesel-assimilating bacterial isolates. | six diesel-degrading bacterial strains were isolated from oil-polluted sites located in central taiwan. the floating activity of the isolates in an oil-supplemented liquid medium was monitored. cell-surface hydrophobicity as well as cell-free and cell-residue emulsification activities were also investigated. three isolates, identified as gordonia alkanivorans cc-jg 39, rhodococcus erythropolis cc-bc 04, and r. erythropolis cc-bc 11, were found to float and grow near the diesel layer on the surfa ... | 2005 | 16233818 |
| bacterial diversity in aortic aneurysms determined by 16s ribosomal rna gene analysis. | aortic aneurysms are common vascular conditions that cause considerable morbidity and mortality. understanding of the mechanisms involved in the pathogenesis of the condition remains limited. recently, infection has been suggested as possible contributor in the development of the disease. the aim of the present study was to examine aortic aneurysms for the presence of bacterial dna using polymerase chain reaction (pcr) targeting the 16s ribosomal rna (rrna) gene, followed by cloning and sequenci ... | 2006 | 17098542 |
| n-acyl homoserine lactones are degraded via an amidolytic activity in comamonas sp. strain d1. | comamonas strain d1 enzymatically inactivates quorum-sensing (qs) signal molecules of the n-acyl homoserine lactone (n-ahsl) family, and exhibits the broadest inactivation range of known bacteria. it degrades n-ahsl with acyl-side chains ranging from 4 to 16 carbons, with or without 3-oxo or 3-hydroxy substitutions. n-ahsl degradation yields hsl but not n-acyl homoserine: strain d1 therefore harbors an amidohydrolase activity. strain d1 is the fifth bacterium species in which an n-ahsl amidohydr ... | 2007 | 17136382 |
| [degradation of p-toluenesulfonate by immobilized comamonas testosteroni bs1310 (pbs1010) cells]. | parameters of degradation of p-toluenesulfonate (ts) by free and agar-embedded comamonas testosteroni bs1310 (pbs1010) cells were determined. the maximum rate of ts degradation was 25% lower in by immobilized than free cells, equaling 11 nmol x min(-1) x mg(-1) cells. degradation of ts by both free and immobilized cells was associated with molecular oxygen consumption (molar ratio, 1 : 2). in a plug-flow reactor, the degradation rate was 10.4 nmol x min(-1) x mg(-1) cells. the results can be app ... | 2005 | 16240649 |
| detection and identification of bacteria using antibiotic susceptibility and a multi-array electrochemical sensor with pattern recognition. | this work proposes the use of amperometric signals generated by a 96-well multi-array dissolved oxygen multi-electrode sensor (dox) coupled with principal component analysis for continuous monitoring, identification and differentiation of bacteria. two types of differentiation mechanisms were tested: (1) direct monitoring of respiratory activity via oxygen consumption and (2) quantification of the effect of three broad-spectrum antibiotics on bacteria growth and respiration over time. five speci ... | 2007 | 17169547 |
| plant-microbe association for rhizoremediation of chloronitroaromatic pollutants with comamonas sp. strain cnb-1. | comamonas sp. strain cnb-1, isolated from activated sludge and having a strong ability to degrade 4-chloronitrobenzene (4cnb), was applied for rhizoremediation of 4cnb-polluted soil through association with alfalfa. confocal laser scanning microscopy revealed that strain cnb-1 successfully colonized alfalfa roots. determination of strain cnb-1 populations by cultivation method and by quantitative competitive pcr technique targeting the chloronitrobenzene nitroreductase gene showed that the popul ... | 2007 | 17222144 |
| diversity of denitrifying bacteria isolated from daejeon sewage treatment plant. | the diversity of the denitrifying bacterial populations in daejeon sewage treatment plant was examined using a culture-dependent approach. of the three hundred and seventy six bacterial colonies selected randomly from agar plates, thirty-nine strains that showed denitrifying activity were selected and subjected to further analysis. according to the morphological and biochemical properties, the thirty nine isolates were divided into seven groups. this grouping was supported by an unweighted pair ... | 2005 | 16273028 |
| a novel deaminase involved in chloronitrobenzene and nitrobenzene degradation with comamonas sp. strain cnb-1. | comamonas sp. strain cnb-1 degrades nitrobenzene and chloronitrobenzene via the intermediates 2-aminomuconate and 2-amino-5-chloromuconate, respectively. deamination of these two compounds results in the release of ammonia, which is used as a source of nitrogen for bacterial growth. in this study, a novel deaminase was purified from comamonas strain cnb-1, and the gene (cnbz) encoding this enzyme was cloned. the n-terminal sequence and peptide fingerprints of this deaminase were determined, and ... | 2007 | 17259310 |
| detection of plasmid transfer from pseudomonas fluorescens to indigenous bacteria in soil by using bacteriophage phir2f for donor counterselection. | the transfer of a genetically marked derivative of plasmid rp4, rp4p, from pseudomonas fluorescens to members of the indigenous microflora of the wheat rhizosphere was studied by using a bacteriophage that specifically lyses the donor strain and a specific eukaryotic marker on the plasmid. transfer of rp4p to the wheat rhizosphere microflora was observed, and the number of transconjugants detected was approximately 10 transconjugants per g of soil when 10 donor cells per g of soil were added; tr ... | 1991 | 16348599 |
| purification and properties of a polyester polyurethane-degrading enzyme from comamonas acidovorans tb-35. | a polyester polyurethane (pur)-degrading enzyme, pur esterase, derived from comamonas acidovorans tb-35, a bacterium that utilizes polyester pur as the sole carbon source, was purified until it showed a single band in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). this enzyme was bound to the cell surface and was extracted by addition of 0.2% n,n-bis(3-d-gluconamidopropyl)deoxycholamide (deoxy-bigchap). the results of gel filtration and sds-page showed that the pur esteras ... | 1998 | 16349494 |
| functional analysis of unique class ii insertion sequence is1071. | various xenobiotic-degrading genes on many catabolic plasmids are often flanked by two copies of an insertion sequence, is1071. this 3.2-kb is element has long (110-bp) terminal inverted repeats (irs) and a transposase gene that are phylogenetically related to those of the class ii transposons. however, the transposition mechanism of is1071 has remained unclear. our study revealed that is1071 was only able to transpose at high frequencies in two environmental beta-proteobacterial strains, comamo ... | 2006 | 16391056 |
| influence of material and tube size on duwls contamination in a pilot plant. | numerous studies have shown that the water discharged from dental unit waterlines (duwls) contains high densities of bacteria, especially non-fermenting gram negative bacteria. the aim of the present study was to investigate how the material (polyethylene-pe and polytetrafluorethylene-ptfe) and size (1.6 and 4.0 mm) of 4 waterlines in a pilot plant influence the level of contamination in the output water. the water contamination was assessed by analyzing the trend of the heterotrophic plate coun ... | 2007 | 17319597 |
| bacterial diversity in mine tailings compared by cultivation and cultivation-independent methods and their resistance to lead and cadmium. | to examine bacterial community composition in rhizosphere of plants colonizing on mine tailings and phylogenetic differences between subcommunities resistant to different metals, we constructed four clone libraries of 16s rdna sequences. one was amplified directly from tailing microbial dna (named as ci library) and three from cultures on the plates containing of 0.5 mm cdcl(2) (cd library), 2 mm pb (no(3))(2) (pb library), and without any metals (cw library). in total, nine bacterial divisions ... | 2007 | 17333426 |
| comamonas odontotermitis sp. nov., isolated from the gut of the termite odontotermes formosanus. | a bacterial strain, designated dant 3-8(t), isolated from the gut of the termite odontotermes formosanus, was investigated by a polyphasic taxonomic approach. the cells were rod-shaped, gram-negative, non-pigmented, non-spore-forming and non-fermentative. phylogenetic analyses using the 16s rrna gene sequence showed that the strain formed a monophyletic branch towards the periphery of the evolutionary radiation occupied by the genus comamonas, its closest neighbours being comamonas testosteroni ... | 2007 | 17392226 |
| presumed pseudobacteremia outbreak resulting from contamination of proportional disinfectant dispenser. | reported here are the microbiological and epidemiological details of a presumed outbreak of aerobic gram-negative bacilli infections affecting 19 hematological patients, which was traced to contaminated disinfectant. over a 5-month period, the following organisms were isolated from the blood cultures of 19 neutropenic patients: pseudomonas fluorescens (n = 13), achromobacter xylosoxidans (n = 12), comamonas testosteroni (n = 2) or stenotrophomonas maltophilia (n = 1). the affected patients were ... | 2007 | 17393202 |
| treatment of naphthalene-2-sulfonic acid from tannery wastewater by a granular activated carbon fixed bed inoculated with bacterial isolates arthrobacter globiformis and comamonas testosteroni. | the kinetics of naphthalene-2-sulfonic acid (2-nsa) adsorption by granular activated carbon (gac) were measured and the relationships between adsorption, desorption, bioavailability and biodegradation assessed. the conventional langmuir model fitted the experimental sorption isotherm data and introduced 2-nsa degrading bacteria, established on the surface of the gac, did not interfere with adsorption. the potential value of gac as a microbial support in the aerobic degradation of 2-nsa by arthro ... | 2006 | 16427119 |
| novel partial reductive pathway for 4-chloronitrobenzene and nitrobenzene degradation in comamonas sp. strain cnb-1. | comamonas sp. strain cnb-1 grows on 4-chloronitrobenzene (4-cnb) and nitrobenzene as sole carbon and nitrogen sources. in this study, two genetic segments, cnbb-orf2-cnba and cnbr-orf1-cnbcacbdefghi, located on a newly isolated plasmid, pcnb1 (ca. 89 kb), and involved in 4-cnb/nitrobenzene degradation, were characterized. seven genes (cnba, cnbb, cnbca, cnbcb, cnbd, cnbg, and cnbh) were cloned and functionally expressed in recombinant escherichia coli, and they were identified as encoding 4-cnb ... | 2006 | 16517619 |
| microbial analysis of soil and groundwater from a gasworks site and comparison with a sequenced biological reactive barrier remediation process. | to investigate the distribution of a polymicrobial community of biodegradative bacteria in (i) soil and groundwater at a former manufactured gas plant (fmgp) site and (ii) in a novel sequential reactive barrier (serebar) bioremediation process designed to bioremediate the contaminated groundwater. | 2007 | 17448158 |
| characterization of the terephthalate degradation genes of comamonas sp. strain e6. | we isolated comamonas sp. strain e6, which utilizes terephthalate (tpa) as the sole carbon and energy source via the protocatechuate (pca) 4,5-cleavage pathway. two almost identical tpa degradation gene clusters, tphricia2ia3ibia1i and tphriiciia2iia3iibiia1ii, were isolated from this strain. based on amino acid sequence similarity, the genes tphr, tphc, tpha2, tpha3, tphb, and tpha1 were predicted to code, respectively, for an iclr-type transcriptional regulator, a periplasmic tpa binding recep ... | 2006 | 16517628 |
| characterization of efficient aerobic denitrifiers isolated from two different sequencing batch reactors by 16s-rrna analysis. | by adopting two sequencing batch reactors (sbrs) a and b, nitrate as the substrate, and the intermittent aeration mode, activated sludge was domesticated to enrich aerobic denitrifiers. the phs of reactor a were approximately 6.3 at dos 2.2-6.1 mg/l for a carbon source of 720 mg/l cod; the phs of reactor b were 6.8-7.8 at dos 2.2-3.0 mg/l for a carbon source of 1500 mg/l cod. both reactors maintained an influent nitrate concentration of 80 mg/l no3- -n. when the total inorganic nitrogen (tin) re ... | 2007 | 17630129 |
| analysis of 525 samples to determine the usefulness of pcr amplification and sequencing of the 16s rrna gene for diagnosis of bone and joint infections. | the 16s rrna gene pcr in the diagnosis of bone and joint infections has not been systematically tested. five hundred twenty-five bone and joint samples collected from 525 patients were cultured and submitted to 16s rrna gene pcr detection of bacteria in parallel. the amplicons with mixed sequences were also cloned. when discordant results were observed, culture and pcr were performed once again. bacteria were detected in 139 of 525 samples. culture and 16s rrna gene pcr yielded identical documen ... | 2006 | 16517890 |
| nickel-resistant bacteria from anthropogenically nickel-polluted and naturally nickel-percolated ecosystems. | dna fragments harboring the nickel resistance determinants from bacteria isolated from anthropogenically polluted ecosystems in europe and zaire were compared with those harboring the nickel resistance determinants from bacteria isolated from naturally nickel-percolated soils from new caledonia by dna-dna hybridization. the biotinylated dna probes were derived from the previously described alcaligenes eutrophus ch34, alcaligenes xylosoxidans 31a, alcaligenes denitrificans 4a-2, and klebsiella ox ... | 1995 | 16535048 |
| oxidative pathway for the biodegradation of nitrobenzene by comamonas sp. strain js765. | previous studies have shown that the biodegradation of nitrobenzene by pseudomonas pseudoalcaligenes js45 proceeds by the reduction of nitrobenzene through nitrosobenzene and hydroxylaminobenzene, followed by rearrangement to 2-aminophenol, which then undergoes meta ring cleavage. we report here the isolation of a comamonas sp. that uses an oxidative pathway for the complete mineralization of nitrobenzene. the isolate, designated strain js765, uses nitrobenzene as a sole source of carbon, nitrog ... | 1995 | 16535050 |
| comamonas testosteroni bacteremia in a patient with perforated acute appendicitis. short communication. | comamonas testosteroni is an uncommon isolate in the clinical laboratory as a human pathogen. c. testosteroni most commonly emerges in abdominal pathologies especially in perforated appendicitis. in turkey we report first time a case of bacteremia due to this organism, in a 22-year-old man with perforated acute appendicitis. the organism was shown to be susceptible to routine antibiotics so it was easily eliminated even after having caused a bacteremia. | 2007 | 17896478 |
| production of r-(-)-ketoprofen from an amide compound by comamonas acidovorans kpo-2771-4. | r-(-)-2-(3(prm1)-benzoylphenyl)propionic acid [r-(-)-ketoprofen] was produced from racemic 2-(3(prm1)-benzoylphenyl)propionamide (keto-amide) by the isolated bacterial strain comamonas acidovorans kpo-2771-4. sodium fumarate as the carbon source and 2-azacyclononanone or isobutyronitrile as the enhancer in the culture medium were effective for bacterial growth and the enhancement of r-(-)-ketoprofen-producing activity. r-(-)-ketoprofen produced from the keto-amide by resting cells was present in ... | 1996 | 16535206 |
| microbial succession during a field evaluation of phenol and toluene as the primary substrates for trichloroethene cometabolism. | microbial community composition and succession were studied in an aquifer that was amended with phenol, toluene, and chlorinated aliphatic hydrocarbons to evaluate the effectiveness of these aromatic substrates for stimulating trichloroethene (tce) bioremediation. samples were taken after the previous year's field studies, which used phenol as the primary substrate, and after three successive monthly treatments of phenol plus 1,1-dichloroethene (1,1-dce) plus tce, phenol plus tce, and toluene pl ... | 1997 | 16535576 |
| testing electrostatic complementarity in enzyme catalysis: hydrogen bonding in the ketosteroid isomerase oxyanion hole. | a longstanding proposal in enzymology is that enzymes are electrostatically and geometrically complementary to the transition states of the reactions they catalyze and that this complementarity contributes to catalysis. experimental evaluation of this contribution, however, has been difficult. we have systematically dissected the potential contribution to catalysis from electrostatic complementarity in ketosteroid isomerase. phenolates, analogs of the transition state and reaction intermediate, ... | 2006 | 16602823 |
| change of collision efficiency with distance in bacterial transport experiments. | understanding the mechanisms of bacterial transport in aquifers is important in developing bioremediation strategies. collision efficiency (alpha) is one important parameter used in modeling bacterial transport. this study was undertaken to measure change in alpha with distance by performing a bacterial transport experiment in oyster, virginia. following injection of a bacterium, comamonas sp., into a well, water samples were collected at various distances along the flowpath and injected into co ... | 2006 | 16681522 |
| microbial community profiling in cis- and trans-dichloroethene enrichment systems using denaturing gradient gel electrophoresis. | the effective and accurate assessment of the total microbial community diversity is one of the primary challenges in modem microbial ecology, especially for the detection and characterization of unculturable populations and populations with a low abundance. accordingly, this study was undertaken to investigate the diversity of the microbial community during the biodegradation of cis- and trans-dichloroethenes in soil and wastewater enrichment cultures. community profiling using pcr targeting the ... | 2007 | 18051265 |
| metabolism of isovanillate, vanillate, and veratrate by comamonas testosteroni strain br6020. | in comamonas testosteroni strain br6020, metabolism of isovanillate (ivan; 3-hydroxy-4-methoxybenzoate), vanillate (van; 4-hydroxy-3-methoxybenzoate), and veratrate (ver; 3,4-dimethoxybenzoate) proceeds via protocatechuate (pca; 3,4-dihydroxybenzoate). a 13.4-kb locus coding for the catabolic enzymes that channel the three substrates to pca was cloned. o demethylation is mediated by the phthalate family oxygenases ivaa (converts ivan to pca and ver to van) and vana (converts van to pca and ver t ... | 2006 | 16707678 |
| oxidative stress by biphenyl metabolites induces inhibition of bacterial cell separation. | cell-cell separation of a polychlorinated biphenyl (pcb)-degrading bacterium comamonas testosteroni tk102 was monitored by flow cytometry. when monohydroxy metabolites of biphenyl (bp) (2-hydroxybiphenyl and 3-hydroxybiphenyl) were added to the culture, cell-cell separation of strain tk102 was inhibited at stationary phase. this inhibition was reproduced on non-pcb degrading bacteria such as pseudomonas putida ppy101 and escherichia coli mv1184, but was not observed on pseudomonas aeruginosa pao ... | 2006 | 16733731 |
| comparative phylogenies of burkholderia, ralstonia, comamonas, brevundimonas and related organisms derived from rpob, gyrb and rrs gene sequences. | phylogenetic analysis of strains from burkholderia, ralstonia, cupriavidus, comamonas, delftia, acidovorax, brevundimonas, herbaspirillum huttiense and "pseudomonas butanovora" was performed based on the protein-coding genes rpob and gyrb and on the 16s rrna-coding gene rrs. overall, the phylogenies deduced from the three genes were concordant among themselves and with current taxonomy. however, a few differences among individual gene phylogenies were noted. for example, the separation of cupria ... | 2008 | 18280706 |
| degradation characteristics and metabolic pathway of 17alpha-ethynylestradiol by sphingobacterium sp. jcr5. | a 17alpha-ethynylestradiol (ee2)-degrading bacterium was isolated from the activated sludge of the wastewater treatment plant (wwtp) of an oral contraceptives producing factory in beijing, china. on the basis of its morphology, biochemical properties and the 16s rdna sequence analysis, this strain was identified as sphingobacterium sp. jcr5. this strain grew on ee2 as sole source of carbon and energy, and metabolized up to 87% of the substrate added (30 mgl-1) within 10 d at 30 degrees c. in add ... | 2007 | 16766017 |
| identification of acetate- or methanol-assimilating bacteria under nitrate-reducing conditions by stable-isotope probing. | stable-isotope probing (sip) was used to identify acetate- or methanol-assimilating bacteria under nitrate-reducing conditions in activated sludge. a sludge sample obtained from wastewater treatment systems was incubated in a denitrifying batch reactor fed with synthetic wastewater containing [(13)c]acetate or [(13)c]methanol as the main carbon source and nitrate as the electron acceptor. we analyzed how growth of bacterial populations was stimulated by acetate or methanol as the external carbon ... | 2006 | 16897304 |
| novel organization of catechol meta pathway genes in the nitrobenzene degrader comamonas sp. js765 and its evolutionary implication. | the catechol meta cleavage pathway is one of the central metabolic pathways for the degradation of aromatic compounds. a novel organization of the pathway genes, different from that of classical soil microorganisms, has been observed in sphingomonas sp hv3 and pseudomonas sp. dj77. in a comamonas sp. js765, cdoe encoding catechol 2,3-dioxygenase shares a common ancestry only with tdnc of a pseudomonas putida strain, while codg encoding 2-hydroxymuconic semialdehyde dehydrogenase shows a higher d ... | 2007 | 16947040 |
| architecture and spatial organization in a triple-species bacterial biofilm synergistically degrading the phenylurea herbicide linuron. | members of a triple-species 3-(3,4-dichlorophenyl)-1-methoxy-1-methyl urea (linuron)-mineralizing consortium, i.e. the linuron- and 3,4-dichloroaniline-degrading variovorax sp. wdl1, the 3,4-dichloroaniline-degrading comamonas testosteroni wdl7 and the n,o-dimethylhydroxylamine-degrading hyphomicrobium sulfonivorans wdl6, were cultivated as mono- or multi-species biofilms in flow cells irrigated with selective or nonselective media, and examined with confocal laser scanning microscopy. in contra ... | 2008 | 18373685 |
| genetic diversity of endophytic bacteria which could be find in the apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants. | the genetic diversity of 29 endophytic bacterial strains isolated from apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants grown in cuba was analysed by two primers-ramdom amplified polymorphic dna fingerprinting (tp-rapd) and 16s rrna gene sequencing. the strains were distributed into 17 groups on the basis of their tp-rapd patterns, and a representative strain from each group was subjected to 16s rrna gene sequencing. analysis of these sequences showed that the is ... | 2008 | 18383223 |
| 4-chlorobenzoate uptake in comamonas sp. strain dj-12 is mediated by a tripartite atp-independent periplasmic transporter. | the fcb gene cluster involved in the hydrolytic dehalogenation of 4-chlorobenzoate is organized in the order fcbb-fcba-fcbt1-fcbt2-fcbt3-fcbc in comamonas sp. strain dj-12. the genes are operonic and inducible with 4-chloro-, 4-iodo-, and 4-bromobenzoate. the fcbt1, fcbt2, and fcbt3 genes encode a transporter in the secondary trap (tripartite atp-independent periplasmic) family. an fcbt1t2t3 knockout mutant shows a much slower growth rate on 4-chlorobenzoate compared to the wild type. 4-chlorobe ... | 2006 | 17041053 |
| crystal structure of 3-hydroxybenzoate hydroxylase from comamonas testosteroni has a large tunnel for substrate and oxygen access to the active site. | the 3-hydroxybenzoate hydroxylase (mhbh) from comamonas testosteroni kh122-3s is a single-component flavoprotein monooxygenase, a member of the glutathione reductase (gr) family. it catalyzes the conversion of 3-hydroxybenzoate to 3,4-dihydroxybenzoate with concomitant requirements for equimolar amounts of nadph and molecular oxygen. the production of dihydroxy-benzenoid derivative by hydroxylation is the first step in the aerobic degradation of various phenolic compounds in soil microorganisms. ... | 2006 | 17045293 |
| characterization of mobr, the 3-hydroxybenzoate-responsive transcriptional regulator for the 3-hydroxybenzoate hydroxylase gene of comamonas testosteroni kh122-3s. | comamonas testosteroni kh122-3s is an aerobic soil bacterium that utilizes 3-hydroxybenzoate as a sole carbon and energy source. in this strain, 3-hydroxybenzoate hydroxylase (moba) acts on the initial step of the degradation to produce 3,4-dihydroxybenzoate, which is subsequently subjected to the meta-cleavage pathway leading to tricarboxylic acid cycle intermediates. gene walking analysis of the upstream region of moba revealed an open reading frame (mobr) that encodes a transcriptional regula ... | 2006 | 17046018 |
| the expression and diversity of catalases in isolates of genus comamonas in response to the oxidative stress of a polluted environment. | we have evaluated the role of monofunctional heme-containing catalase encoded by cat-1 gene from the soil bacterium comamonas terrigena n3h in the response to various forms of oxidative stress. our results indicate that this constitutively expressed catalase represents the major source for the defence of comamonas terrigena cells against toxic peroxides but the cells can express also a second form of catalase that is bigger and its regulation is probably more complicated. the sequence analysis c ... | 2006 | 17066334 |
| membrane topology of aspartate:alanine antiporter aspt from comamonas testosteroni. | we cloned the aspt gene encoding the l-aspartate:l-alanine antiporter asptct in comamonas testosteroni genomic dna. analysis of the nucleotide sequence revealed that c. testosteroni has an asp operon containing aspt upstream of the l-aspartate 4-decarboxylase gene, and that the gene order of the asp operon of c. testosteroni is the inverse of that of tetragenococcus halophilus. we used proteoliposomes to confirm the transport processes of asptct. to elucidate the two-dimensional structure of asp ... | 2007 | 17158863 |
| understanding oligomerization in 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from comamonas testosteroni: an in silico approach and evidence for an active protein. | 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-hsd/cr) from comamonas testosteroni belongs to the short chain dehydrogenase/reductase (sdr) protein superfamily and catalyzes the oxidoreduction of a variety of steroid substrates, including the steroid antibiotic fusidic acid. the enzyme also mediates the carbonyl reduction of non-steroidal aldehydes and ketones such as a novel insecticide. it is suggested that 3alpha-hsd/cr contributes to the bioremediation of natural and syntheti ... | 2007 | 17258342 |
| microbial degradation of quinoline: kinetic studies with comamonas acidovorans dsm 6426. | the microbial degradation of quinoline by comamonas acidovorans was studied in a laboratory scale stirred tank reactor. in continuous culture experiments using quinoline as a sole source of carbon and nitrogen, it was shown by means of mass balances that quinoline was converted completely to biomass, carbon dioxide, and ammonia. degradation rates up to 0.7 g/l h were obtained. measured yield coefficients y(x/s) for quinoline were about 0.7 g/g, which is in agreement with the theoretical value fo ... | 1993 | 18613080 |
| influence of sustainability and immigration in assembling bacterial populations of known size and function. | the rational assembly of microbial communities to perform desired functions would be of great practical benefit to society. broadly speaking, there are two major theoretical foundations for microbial community assembly: one based on island biogeography theory and another based on niche theory. in this study, we compared a parameter from each theory (immigration rate and sustainability, respectively) to ascertain which was more influential in establishing a functional bacterial population in phen ... | 2007 | 17264996 |
| influence of cobalt substitution on the activity of iron-type nitrile hydratase: are cobalt type nitrile hydratases regulated by carbon monoxide? | comamonas testosteroni ni1 nitrile hydratase is a fe-type nitrile hydratase whose native and recombinant forms are identical. here, the iron of ni1 nitrile hydratase was replaced by cobalt using a chaperone based escherichia coli expression system. cobalt (coni1) and iron (feni1) enzymes share identical vmax (30 nmol min(-1) mg(-1)) and km (200 microm) toward their substrate and identical ki values for the known competitive inhibitors of feni1. however, nitrophenols used as inhibitors do display ... | 2007 | 17267045 |
| performance of three phase fluidized bed reactor for quinoline degradation on various supports at steady state and dynamic conditions. | quinoline degradation by comamonas acidovorans was investigated in a three phase fluidized bed reactor at dilution rates below and above the critical value (micro(max) = 0.42 h(-1)). quinoline was used as the sole source of carbon, nitrogen, and energy. two attachment carriers, polyurethane foam (bayvitec) and modified cellulose (aquacel), and a gel entrapment carrier (polyvinyl alcohol) were studied and compared with regard to their effectiveness to immobilize cells. attachment and biofilm form ... | 1997 | 18636645 |
| electricity generation from cellulose by rumen microorganisms in microbial fuel cells. | in microbial fuel cells (mfcs) bacteria generate electricity by mediating the oxidation of organic compounds and transferring the resulting electrons to an anode electrode. the objective of this study was to test the possibility of generating electricity with rumen microorganisms as biocatalysts and cellulose as the electron donor in two-compartment mfcs. the anode and cathode chambers were separated by a proton exchange membrane and graphite plates were used as electrodes. the medium in the ano ... | 2007 | 17274068 |
| [study on the structure and function of a stable methane-oxidizing mixed microbial consortium]. | from an agricultural sample taken in chongqing, a stable methane-oxidizing mixed microbial consortium was established by enrichment culture with methane as a sole source of carbon and energy. the mixed consortium showed high capability of phenol degradation and 1,2-epoxypropane production from propene. more than 99% of phenol at an initial concentration of 600mg/l could be degraded by the mixed microbial consortium after 11 h of cultivation. the productivity of 1, 2-epoxypropane could be increas ... | 2007 | 17436634 |
| potential of the adhesion of bacteria isolated from drinking water to materials. | heterotrophic bacteria (11 genera, 14 species, 25 putative strains) were isolated from drinking water, identified either biochemically or by partial 16s rdna gene sequencing and their adherence characteristics were determined by two methods: i. thermodynamic prediction of adhesion potential by measuring hydrophobicity (contact angle measurements) and ii. by measuring adherence to eight different substrata (asi 304 and 316 stainless steel, copper, polyvinyl chloride, polypropylene, polyethylene, ... | 2007 | 17440920 |
| high crystallizability under air-exclusion conditions of the full-length lysr-type transcriptional regulator tsar from comamonas testosteroni t-2 and data-set analysis for a miras structure-solution approach. | the full-length lysr-type transcriptional regulator tsar from comamonas testosteroni t-2 was heterologously overexpressed in escherichia coli, purified and stabilized under conditions that favoured its rapid crystallization using the microbatch-under-oil technique. the purified protein was highly crystallizable and two different crystal forms were readily obtained. however, only monoclinic crystals gave diffraction beyond 2 a and there was a slight variation in unit-cell parameters between cryst ... | 2008 | 18678953 |
| establishment and characterization of dual-species biofilms formed from a 3,5-dinitrobenzoic-degrading strain and bacteria with high biofilm-forming capabilities. | in this study, the possibility of establishing a dual-species biofilm from a bacterium with a high biofilm-forming capability and a 3,5-dinitrobenzoic acid (3,5-dnba)-degrading bacterium, comamonas testosteroni a3, was investigated. our results showed that the combinations of strain a3 with each of five strains with a high biofilm-forming capability (pseudomonas sp. m8, pseudomonas putida m9, bacillus cereus m19, pseudomonas plecoglossicida m21 and aeromonas hydrophila m22) presented different l ... | 2008 | 18034834 |
| comamonas composti sp. nov., isolated from food waste compost. | a bacterial strain designated yy287(t), isolated from food waste compost, was investigated by polyphasic taxonomic approach. the cells were rod-shaped, gram-negative, non-pigmented, non-spore-forming and non-fermentative. phylogenetic analyses using the 16s rrna gene sequence showed that the strain formed a monophyletic branch towards the periphery of the evolutionary radiation occupied by the genus comamonas; its closest neighbours were the type strains comamonas testosteroni dsm 50244(t) (96.5 ... | 2008 | 18175717 |
| biodegradation potential and bacterial diversity of a petrochemical wastewater treatment plant in iran. | an activated sludge treatment was evaluated for its effectiveness in cleaning up a petrochemical wastewater in iran. for assessing biodegradation potential of activated sludge, seven characteristics of wastewater (temperature, ph, dissolved oxygen, chemical oxygen demand, concentrations of ethylene dichloride, vinyl chloride, and total hydrocarbons) were monitored during six months. it was shown that dominant pollutants in order of magnitude were normal-alkanes (c(10)-c(21)), aromatics, and poly ... | 2008 | 18255283 |
| different bacterial strategies to degrade taurocholate. | aerobic enrichment cultures with taurocholate or alkanesulfonates as sole sources of carbon and energy for growth were successful and yielded nine bacterial isolates, all of which utilized taurocholate. growth was complex and involved not only many, usually transient, excretion products but also sorption of taurocholate and cholate to cells. three metabolic strategies to dissimilate taurocholate were elucidated, all of which involved bile salt hydrolase cleaving taurocholate to cholate and tauri ... | 2008 | 18320171 |
| experience of comamonas acidovorans keratitis with delayed onset and treatment response in immunocompromised cornea. | to report 2 cases of comamonas acidovorans keratitis in immunocompromised cornea. | 2008 | 18323706 |
| biodegradation of direct red 5b, a textile dye by newly isolated comamonas sp. uvs. | soil samples collected from the vicinity of "manpasand textile industry", located near ichalkaranji, india were studied for screening and isolation of bacterial strains capable of degradation of textile dyes. a potential strain was selected on the basis of rapid dye degradation and later identified as comamonas sp. uvs. comamonas sp. uvs showed 100% decolorization of direct red 5b (dr5b) dye at 40 degrees c and ph 6.5. the maximum direct red 5b concentration decolorized was 1,100 mg/l in nutrien ... | 2008 | 18329800 |
| examination and expansion of the substrate range of m-hydroxybenzoate hydroxylase. | the gene encoding m-hydroxybenzoate hydroxylase (moba) was cloned from comamonas testosteroni gz39. moba converts m-hydroxybenzoate and to a lesser extent p-hydroxybenzoate to protocatechuate. to explore the structural and functional relationships in phenolic acid monooxygenases, moba was subjected to in vitro mutagenesis by error-prone pcr and the mutant mobas were screened for their ability to oxidize phenol or 3-aminophenol. a mutant moba with a single v257a substitution was able to transform ... | 2008 | 18417078 |
| analyzing the catalytic mechanism of the fe-type nitrile hydratase from comamonas testosteroni ni1. | in order to gain insight into the catalytic mechanism of fe-type nitrile hydratases (nhase), the ph and temperature dependence of the kinetic parameters k cat, k m, and k cat/ k m along with the solvent isotope effect were examined for the fe-type nhase from comamonas testosteroni ni1 ( ctnhase). ctnhase was found to exhibit a bell-shaped curve for plots of relative activity vs ph over ph values 4-10 for the hydration of acrylonitrile and was found to display maximal activity at ph approximately ... | 2008 | 18942853 |
| nucleotide sequence of plasmid pcnb1 from comamonas strain cnb-1 reveals novel genetic organization and evolution for 4-chloronitrobenzene degradation. | the nucleotide sequence of a new plasmid pcnb1 from comamonas sp. strain cnb-1 that degrades 4-chloronitrobenzene (4cnb) was determined. pcnb1 belongs to the incp-1beta group and is 91,181 bp in length. a total of 95 open reading frames appear to be involved in (i) the replication, maintenance, and transfer of pcnb1; (ii) resistance to arsenate and chromate; and (iii) the degradation of 4cnb. the 4cnb degradative genes and arsenate resistance genes were located on an extraordinarily large transp ... | 2007 | 17526790 |
| ligand specificity of mobr, a transcriptional regulator for the 3-hydroxybenzoate hydroxylase gene of comamonas testosteroni kh122-3s. | mobr from comamonas testosteroni kh122-3s is a member of the marr family of transcriptional regulators and functions as a repressor for the moba gene that encodes a 3-hydroxybenzoate 4-hydroxylase. 3-hydroxybenzoate binds to mobr as a ligand, resulting in an efficient induction of moba. various 3-hydroxybenzoate analogues were examined for their inducibilities using the moba::lacz transcriptional fusion system. beta-galactosidase was induced by the addition of 2,3-dihydroxybenzoate or 3,5-dihydr ... | 2007 | 17707338 |
| microbial iron redox cycling in a circumneutral-ph groundwater seep. | the potential for microbially mediated redox cycling of iron (fe) in a circumneutral-ph groundwater seep in north central alabama was studied. incubation of freshly collected seep material under anoxic conditions with acetate-lactate or h(2) as an electron donor revealed the potential for rapid fe(iii) oxide reduction (ca. 700 to 2,000 micromol liter(-1) day(-1)). fe(iii) reduction at lower but significant rates took place in unamended controls (ca. 300 micromol liter(-1) day(-1)). culture-based ... | 2009 | 19047399 |
| pha-rubber blends: synthesis, characterization and biodegradation. | medium chain length polyhydroxyalkanoates (mcl-pha) and different rubbers; namely natural rubber, nitrile rubber and butadiene rubber were blended at room temperature using solution blending technique. blends constituted 5%, 10% and 15% of mcl-pha in different rubbers. thermogravimetric analysis of mcl-pha showed the melting temperature of the polymer around 50 degrees c. thermal properties of the synthesized blend were studied by differential scanning calorimetry which confirmed effective blend ... | 2008 | 17764931 |
| mechanism of proton transfer in the 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from comamonas testosteroni. | 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from comamonas testosteroni catalyzes the oxidation of androsterone with nad(+) to form androstanedione and nadh with a concomitant releasing of protons to bulk solvent. to probe the proton transfer during the enzyme reaction, we used mutagenesis, chemical rescue, and kinetic isotope effects to investigate the release of protons. the kinetic isotope effects of (d)v and (d(2)o)v for wild-type enzyme are 1 and 2.1 at pl 10.4 (where l represent ... | 2007 | 17893142 |
| the molecular basis for inhibition of bphd, a c-c bond hydrolase involved in polychlorinated biphenyls degradation: large 3-substituents prevent tautomerization. | the microbial degradation of polychlorinated biphenyls (pcbs) by the biphenyl catabolic (bph) pathway is limited in part by the pathway's fourth enzyme, bphd. bphd catalyzes an unusual carbon-carbon bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (hopda), in which the substrate is subject to histidine-mediated enol-keto tautomerization prior to hydrolysis. chlorinated hopdas such as 3-cl hopda inhibit bphd. here we report that bphd preferentially hydrolyzed a series of 3-substit ... | 2007 | 17932031 |
| thiosulfate oxidation by comamonas sp. s23 isolated from a sulfur spring. | a bacterial isolate s23 capable of oxidizing thiosulfate was isolated from a sulfur spring. strain s23 is gram-negative, aerobic, and motile. the g + c content of dna is 61.4 mol%. the fatty acid composition and phylogenetic analysis of the 16s rrna gene sequence of strain s23 showed that it is related to the members of the genus comamonas, and most closely related to comamonas testosteroni (99.9% sequence similarity). the isolate s23 exhibited thiosulfate oxidation under a mixotrophic growth co ... | 2009 | 19189181 |
| post-operative endophthalmitis due to an unusual pathogen, comamonas testosteroni. | here, we describe the first report of post-operative endophthalmitis due to comamonas testosteroni in an elderly diabetic patient after complicated cataract surgery. the isolate was identified by using mini api strips. the patient was successfully treated with intravitreal ceftazidime and oral ciprofloxacin. | 2009 | 19208890 |
| [biosynthesis of polyhydroxyalkanoates and its metabolic pathway in comamonas sp. strain cnb-1]. | comamonas sp. strain cnb-1 degrades chloronitrbenzene and nitrobenzene for carbon and nitrogen sources. in this study, accumulation of polyhydroxyalkanoic acids (phas) within strain cnb-1 cells was investigated under various conditions. results indicated that strain cnb-1 was able to synthesize pha from various short-chain fatty acid and alcohols, and 57 w% of the dry cell weight (dcw) pha was obtained when valerate and 1,4-butanediol were co-fed. supplements of short-chain alcohols stimulated t ... | 2007 | 17944360 |
| responses to arsenate stress by comamonas sp. strain cnb-1 at genetic and proteomic levels. | comamonas sp. strain cnb-1, a chloronitrobenzene-degrading bacterium, was demonstrated to possess higher arsenate tolerance as compared with the mutant strain cnb-2. pcnb1, a plasmid harboured by cnb-1 but not cnb-2, contained the genetic cluster ars(rpbc)com, which putatively encodes arsenate-resistance regulator, family ii arsenate reductase, arsenite efflux pump and family i arsenate reductase, respectively, in comamonas strain cnb-1. the arsc-negative escherichia coli could gain arsenate res ... | 2007 | 17975079 |
| comamonas granuli sp. nov., isolated from granules used in a wastewater treatment plant. | a gram-negative, motile, rod-shaped, non-spore-forming bacterial strain, designated as ko03(t), was isolated from microbial granules, and was characterized, using a polyphasic approach, in order to determine its taxonomic position. the isolate was positive for catalase and oxidase, but negative for gelatinase and beta-galactosidase. phylogenetic analyses using the 16s rrna gene sequence showed that the strain formed a monophyletic branch towards the periphery of the evolutionary radiation occupi ... | 2008 | 18758728 |
| enzymatic properties of terephthalate 1,2-dioxygenase of comamonas sp. strain e6. | the tpha1 ii and tpha2 ii a3 ii genes of comamonas sp. e6 perhaps code for the terephthalate (tpa) 1,2-dioxygenase (tpado). to characterize e6 tpado, these genes were expressed in a his-tagged form in escherichia coli, and the recombinant proteins were purified. tpado activity was reconstituted from tpha1 ii and tpha2 ii a3 ii, indicating that tpado consists of a reductase (tpha1 ii) and a terminal oxygenase component (tpha2 ii and tpha3 ii). tpha1(ii) contains fad, and the presence of a plant-t ... | 2008 | 18776687 |
| expression of a fused fpg gene in e. coli and engineering a strain comamonas testosteroni zd4-1-fpg for environmental application. | a fused fpg gene composed of phe b and gfp gene, which encoded catechol 2,3-dioxygenase (c23o) and green fluorescence protein (gfp), respectively, was expressed in e. coli to investigate its functional intactness. the expression results showed that c23o activity was detected in the induced bacterial cells and the e. coli cells containing the fusion protein emitted green fluorescence under epifluorescence microscopy, indicating that the fused fpg gene expressed correctly in e. coli. after express ... | 2008 | 18780218 |
| the role of humic substances in the anaerobic reductive dechlorination of 2,4-dichlorophenoxyacetic acid by comamonas koreensis strain cy01. | the role of the humic model compound, anthraquinone-2,6-disulfonate (aqds), in the anaerobic reductive dechlorination of 2,4-dichlorophenoxyacetic acid (2,4-d) by the fe(iii)- and humic substances (hs)-reducing bacterium, comamonas koreensis strain cy01 was investigated. the results taken as a whole indicated that (i) strain cy01 could couple glucose oxidation to 2,4-d reductive dechlorination; (ii) reductive dechlorination of 2,4-d by strain cy01 was greatly stimulated by the addition of aqds; ... | 2009 | 18849114 |
| antibiotic susceptibility profiles of uncommon bacterial species causing severe infections in italy. | this study presents the results of the italian "severe infections project" involving bacteria that can be considered rare causes of disease. we isolated 30 uncommon human pathogens from a total of 60 strains (1.2% of all the isolates). the most frequent sources of uncommon human pathogens were primary bloodstream infections (48.3%) and pneumonia (20%). species such as comamonas testosteroni, enterococcus hirae, kluyvera ascorbata, kluyvera cryocrescens, leclercia adecarboxylata and ochrobactrum ... | 2009 | 19567344 |
| atrazine degradation by a simple consortium of klebsiella sp. a1 and comamonas sp. a2 in nitrogen enriched medium. | a simple consortium consisted of two members of klebsiella sp. a1 and comamonas sp. a2 was isolated from the sewage of a pesticide mill in china. one member of klebsiella sp. a1 is a novel strain that could use atrazine as the sole carbon and nitrogen source. the consortium showed high atrazine-mineralizing efficiency and about 83.3% of 5 g l(-1) atrazine could be mineralized after 24 h degradation. contrary to many other reported microorganisms, the consortium was insensitive to some nitrogenou ... | 2010 | 19603273 |
| persistent wolbachia and cultivable bacteria infection in the reproductive and somatic tissues of the mosquito vector aedes albopictus. | commensal and symbiotic microbes have a considerable impact on the behavior of many arthropod hosts, including hematophagous species that transmit pathogens causing infectious diseases to human and animals. little is known about the bacteria associated with mosquitoes other than the vectorized pathogens. this study investigated wolbachia and cultivable bacteria that persist through generations in ae. albopictus organs known to host transmitted arboviruses, such as dengue and chikungunya. | 2009 | 19633721 |
| regulation of testosterone degradation in comamonas testosteroni. | recently, we have identified a gene encoding a luxr-type factor, teir (testosterone-inducible regulator), which positively regulates steroid degradation in comamonas testosteroni. herein, we demonstrate that teir interacts in vivo with steroid catabolic gene promoters. the presence of testosterone induces a significant teir protein increase at the early logarithmic phase of growth. interestingly, it is not until the early stationary phase where the activation of a steroid-inducible gene promoter ... | 2008 | 18852046 |
| biofilm formation and interactions of bacterial strains found in wastewater treatment systems. | biofilm formation and adherence properties of 13 bacterial strains commonly found in wastewater treatment systems were studied in pure and mixed cultures using a crystal violet microtiter plate assay. four different culture media were used, wastewater, acetate medium, glucose medium and diluted nutrient broth. the medium composition strongly affected biofilm formation. all strains were able to form pure culture biofilms within 24 h in at least one of the tested culture media and three strains we ... | 2008 | 18422628 |
| testosterone-inducible regulator is a kinase that drives steroid sensing and metabolism in comamonas testosteroni. | the mechanism of gene regulation by steroids in bacteria is still a mystery. we use steroid-inducible 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-hsd/cr) as a reporter system to study steroid signaling in comamonas testosteroni. in previous investigations we cloned and characterized the 3alpha-hsd/cr-encoding gene, hsda. in addition, we identified two negative regulator genes (repa and repb) in the vicinity of hsda, the protein products which repress hsda expression on the lev ... | 2008 | 18424443 |
| 15n nmr relaxation studies of y14f mutant of ketosteroid isomerase: the influence of mutation on backbone mobility. | the backbone dynamics of y14f mutant of delta(5)-3-ketosteroid isomerase (ksi) from comamonas testosteroni has been studied in free enzyme and its complex with a steroid analogue, 19-nortestosterone hemisuccinate (19-nths), by 15n nmr relaxation measurements. model-free analysis of the relaxation data showed that the single-point mutation induced a substantial decrease in the order parameters (s2) in free y14f ksi, indicating that the backbone structures of y14f ksi became significantly mobile b ... | 2008 | 18424811 |
| proteomic and molecular investigation on the physiological adaptation of comamonas sp. strain cnb-1 growing on 4-chloronitrobenzene. | comamonas sp. strain cnb-1 can utilize 4-chloronitrobenzene (4cnb) as sole carbon and nitrogen source for growth. previous studies were focused on 4cnb degradative pathway and have showed that cnb-1 contained a plasmid pcnb1 harboring the genes (cnbabcacbdefgh, cnbz) for the enzymes involving in 4cnb degradation, but only three gene products (cnbca, cnbcb, and cnbz) were identified in cnb-1 cells. comamonas strain cnb-2 that lost pcnb1 was not able to grow on 4cnb. in this study, physiological a ... | 2009 | 18509595 |
| the complete genome of comamonas testosteroni reveals its genetic adaptations to changing environments. | members of the gram-negative, strictly aerobic genus comamonas occur in various environments. here we report the complete genome of comamonas testosteroni strain cnb-2. strain cnb-2 has a circular chromosome that is 5,373,643 bp long and has a g+c content of 61.4%. a total of 4,803 open reading frames (orfs) were identified; 3,514 of these orfs are functionally assigned to energy production, cell growth, signal transduction, or transportation, while 866 orfs encode hypothetical proteins and 423 ... | 2009 | 19734336 |
| identification of genes involved in inversion of stereochemistry of a c-12 hydroxyl group in the catabolism of cholic acid by comamonas testosteroni ta441. | comamonas testosteroni ta441 degrades steroids such as testosterone via aromatization of the a ring, followed by meta-cleavage of the ring. in the dna region upstream of the meta-cleavage enzyme gene tesb, two genes required during cholic acid degradation for the inversion of an alpha-oriented hydroxyl group on c-12 were identified. a dehydrogenase, stea, converts 7 alpha,12 alpha-dihydroxyandrosta-1,4-diene-3,17-dione to 7 alpha-hydroxyandrosta-1,4-diene-3,12,17-trione, and a hydrogenase, steb, ... | 2008 | 18539741 |
| [isolation and identification of phenol-degrading strains and the application in biotreatment of phenol-containing wastewater]. | ten phenol-degrading bacterial strains were isolated from mixture of activated sludge and wastewater of a petroleum chemical plant. the five isolates (pd1, pd2, pd6, pd7 and pd39) were identified as pseudomonas sp.,the four (pd4, pd5, pd8 and pd9) as acinetobacter sp.,and the one (pd3) as comamonas sp.by 16s rdna sequence. biodegradation characteristics of phenol, optimal conditions for growth, substrate range, activities of catechol 1,2-dioxygenase and catechol 2,3-dioxygenase, and biotreatment ... | 2008 | 18613524 |
| degradation of quinoline by immobilized comamonas acidovorans in a three-phase airlift reactor. | quinolie degradation by comamonas acidovorans was studied in a continuously operated three-phase airlift reactor. porous glass beads were applied as support matrix for cell imobilization by colonization. under steady-state conditions (s approximately 0), cell attachment was poor at low dilution rates but imporved considerably with increasing dilution rate. conversion of quinoline was investigated below and above the washout for suspended culture (d(crit) = mu(max) = 0.42 h(-1)). with immobilized ... | 1995 | 18623265 |
| comamonas testosteroni meningitis in a homeless man. | we report a case of meningitis caused by comamonas testosteroni in a 54-year-old, alcoholic, homeless man. he, as a pedestrian, was struck by a car and suffered multiple fractures of the facial bones including the left frontal sinus. over the course of 2-week hospitalization, he was clinically diagnosed with multiple cerebral and cerebellar infarcts resulting in altered mental status. he was pronounced dead 15 days after the injury. at the time of autopsy, diffuse purulent meningitis was found o ... | 2008 | 18637874 |