| isolation of brucella organisms from the milk of seronegative cows. | during an investigation of bovine brucellosis in iran, conducted by the razi institute over a twelve-month period, samples of serum and milk were collected simultaneously from 6,472 cows in eight infected herds for serological and bacteriological testing. a total of 1,056 cows were serologically positive and 1,632 of 6,472 milk samples were positive to the milk ring test (mrt). culture of the positive milk samples yielded 397 isolates of brucella, 119 of which came from the 5,686 seronegative co ... | 1990 | 2132709 |
| characterization of a phosphomonoesterase from brucella abortus. | brucellae are facultative intracellular bacterial pathogens that reside primarily in cells of the reticuloendothelial system. the high-speed supernatant obtained after centrifuging a suspension of brucella abortus that had been frozen-thawed and sonicated contained abundant phosphomonoesterase activity, determined by using 4-methylumbelliferylphosphate as the substrate; this enzyme was purified 2,900-fold (yield, 570%) by chromatography on de-52 cellulose and hydroxylapatite columns and high-per ... | 1990 | 2157665 |
| effects of standard and variant strains of infectious bursal disease virus on infections of chickens. | t-cell-mediated and humoral immune responses were measured in chickens infected with standard and variant strains of infectious bursal disease virus. one-day-old and 3-week-old chickens were infected with these viruses and then given sheep rbc, killed brucella abortus strain 19, and newcastle disease virus. appropriate serologic tests were used to monitor the primary and secondary responses to the antigens. lymphoblast transformation assays were performed weekly. the response to the infectious b ... | 1990 | 2167031 |
| cloning, expression, and occurrence of the brucella cu-zn superoxide dismutase. | recently, the complete amino acid sequence of a protein expressed in escherichia coli from cloned brucella abortus dna was reported. on the basis of amino acid homology, this protein was identified as a copper-zinc superoxide dismutase (cu-zn sod) (b. l. beck, l. b. tabatabai, and j. e. mayfield, biochemistry 29:372-376, 1990). we demonstrate in this paper that the sequenced protein is the same as the previously studied salt-extractable protein bcsp20. the plasmid-encoded protein expressed from ... | 1990 | 2201639 |
| the isolation and serology of brucella melitensis in a flock of goats in central rsa. | brucella melitensis biotype 1 was isolated in pure culture from the lungs, liver, spleen, kidney, stomach contents, abomasum and brain of an aborted caprine (boer goat) foetus in the district of cullinan near pretoria. the 18 does and 1 ram in the flock of boer goates were examined serologically by means of the complement fixation (cf) test, using brucella abortus antigen. six weeks later they were examined again, using b. abortus as well as b. melitensis biotype 1 antigens. no significant diffe ... | 1990 | 2216349 |
| development of a cloning system in mycoplasma pulmonis. | a system suitable for recombinant dna manipulation in mycoplasmas was developed using the cloned antibiotic-resistance genes of tn4001 and tn916. an integrative plasmid containing one of the resistance markers was inserted into the genome of mycoplasma pulmonis to form a recipient strain. this was accomplished by transformation and homologous recombination between chromosomal dna sequences cloned onto the integrative plasmid. a second vector, the cloning vector, containing the same plasmid repli ... | 1990 | 2227427 |
| sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting analysis of smooth-lipopolysaccharide heterogeneity among brucella biovars related to a and m specificities. | smooth (s)-lipopolysaccharide (lps) preparations from reference and field strains of several biovars of brucella abortus, b. melitensis, and b. suis were prepared by (i) the hot phenol-water method, (ii) hot sodium dodecyl sulfate extraction and proteinase k digestion, or (iii) dimethyl sulfoxide extraction. these s-lps-enriched fractions were further analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining after periodate oxidation. immunoblots were developed by ... | 1990 | 2229339 |
| microagglutination test for early and specific serodiagnosis of tularemia. | a microagglutination test with safranin-stained francisella tularensis antigen was compared with a conventional tube agglutination test for the serodiagnosis of tularemia. the microagglutination test was performed in round-bottom microtiter plates by using 0.025 ml of the antisera and of the antigen. the antibody titers obtained by using the microagglutination test were 8 to 64 times higher than those seen with the tube agglutination. by the microagglutination test, the serum agglutinins were de ... | 1990 | 2229367 |
| brucella endocarditis: the role of combined medical and surgical treatment. | brucella endocarditis, although a rare complication of brucellosis, is the main cause of death related to this disease. this report describes a case of aortic endocarditis due to brucella abortus in an elderly farmer with known aortic stenosis. urgent valve replacement was performed because of progressive heart failure despite appropriate antimicrobial treatment. the infection was cured with trimethoprim-sulfamethoxazole and rifampin given for 3 months after surgery. a review of the literature r ... | 1990 | 2237111 |
| polymorphism in brucella spp. due to highly repeated dna. | the species of brucella are very closely related, but brucella ovis does not express detectable amounts of a protein, designated bcsp31, that is common to the other species. we studied the lack of expression of bcsp31 by southern analysis. dnas from the b. ovis culture collection strains and field isolates were probed with a 1.3-kb hindiii fragment encoding bcsp31 of brucella abortus. the probe hybridized to a 1.6-kb hindiii fragment of all b. ovis strains tested, showing that the gene is presen ... | 1990 | 2254243 |
| preliminary development of a diagnostic test for brucella using polymerase chain reaction. | a highly sensitive and specific diagnostic test for brucella based on polymerase chain reaction is under development in our laboratory. a commercially available pcr kit was used to create primers that allowed the amplification of a 635 bp fragment of a 43 kda outer membrane protein gene from brucella abortus strain 19. we successfully amplified the cloned gene present in the pms64 plasmid and genomic brucella s19 dna. the amplified dna was easily detected by agarose gel electrophoresis. using bo ... | 1990 | 2272943 |
| effects of local and systemic immunization on serum antibody titres in splenectomized chickens. | the role of spleen in local (per anum) and systemic (intravenous = i.v.) immunization was studied by splenectomizing chickens and immunizing them twice with sheep red blood cells (srbc) and brucella abortus. the number of germinal centers in spleen and the splenic weight were also recorded. splenectomy had no effect on the serum titres evoked by the per anum immunization but it affected the anti-srbc and, to a lesser extent, anti-brucella titres in serum after i.v. immunization. the effect of sp ... | 1990 | 2302348 |
| the mechanism of enhanced intraphagocytic killing of bacteria by liposomes containing antibiotics. | liposomes containing aminoglycosides have been shown to enhance the killing of brucella abortus and staphylococcus aureus inside bovine phagocytic cells. this study examined the mechanism by which liposomes containing aminoglycoside enhance the intracellular killing of bacteria. liposomes with entrapped aminoglycoside were found to significantly enhance the intraphagocytic killing of bacteria in bovine phagocytic cells (in vitro) when compared to free drug. liposomes with entrapped aminoglycosid ... | 1990 | 2336788 |
| autosomal albinism affects immunocompetence in the chicken. | immune responsiveness of three neonatal and juvenile phenotypes, determined by the albinotic c locus, was evaluated. the phenotypes included normally pigmented (c+/-), recessive white (c/ca), and completely amelanotic albinos (ca/ca). no differences in: (1) primary agglutinin levels directed against sheep red blood cells (srbc) or brucella abortus (ba), or (2) cell-mediated immunity, as estimated by in vivo mitogen stimulation, were associated with the albino phenotype. the significant suppressi ... | 1990 | 2338150 |
| antioxidants delay the onset of thyroiditis in obese strain chickens. | dietary iodine has been shown to be important in the induction of thyroiditis in susceptible chicken strains although the underlying mechanism remains unknown. iodine may exert its effects through the formation of reactive oxidative radicals which would cause thyroidal injury and initiate infiltration. we have tested this hypothesis by examining the ability of butylated hydroxyanisole (bha), ethoxyquin, and other antioxidants to prevent thyroiditis in obese strain (os) chickens, a strain that de ... | 1990 | 2401227 |
| treatment of brucella canis and brucella abortus in vitro and in vivo by stable plurilamellar vesicle-encapsulated aminoglycosides. | stable plurilamellar vesicles (splvs) entrapping aminoglycosides were used to treat infections due to brucella species (brucella canis and brucella abortus). splv-entrapped antibiotics effectively eliminated internalized b. canis in cultures of resident murine peritoneal macrophages and internalized b. abortus in cultures of resident guinea pig peritoneal macrophages. in vivo studies demonstrated that splv-entrapped aminoglycosides administered to b. canis-infected mice and b. abortus-infected g ... | 1985 | 2411828 |
| a primary in vitro antibody assay for antigen-specific t-suppressor factor: cross-suppression of tnp-specific antibody responses by tma-specific tsf1. | we have previously shown that phenyltrimethylammonium (tma)-specific, first-order suppressor t cells (ts1) and soluble factors extracted from these cells (tsf1) can suppress delayed-type hypersensitivity (dth) responses. the tsf1, as monitored in the dth system, was characterized and found to be a single-chain, antigen-binding, i-j+, and id+ molecule. to monitor tsf1 in an efficient manner, an in vitro antibody system was developed. the studies show that in vitro stimulation of naive a/j spleen ... | 1985 | 2412707 |
| comparison of brucella abortus and b melitensis antigens for the rose bengal plate test on sera from cattle infected with b abortus biovar-5. | | 1985 | 2414899 |
| chickens bursectomized with mibolerone have ig-positive cells which lack bursal cell specific antigens. | the bursa anlage failed to develop in chicken embryos injected with 5 micrograms mibolerone on the 5th day of embryonation. humoral immune responses in treated birds were studied by challenging them with sheep red blood cells (srbc) and killed brucella abortus (b. abortus) at 4, 5, 6 and 7 weeks of age. control birds responded with igm and igg titers to srbc and b. abortus, whereas mibolerone-treated birds completely failed to respond to b. abortus and their responses to srbc consisted of only i ... | 1985 | 2417401 |
| serological cross-reactions between brucella abortus and yersinia enterocolitica 0:9. | yersinia enterocolitica (ye) 0:9 is an organism of great significance in veterinary medicine largely as a result of its cross-reaction with brucella abortus (ba). boty ye 0:9 and ba possess somatic antigens in common; as a result of which animals exposed to ye 0:9 have an immune response which is distinguishable only with difficulty from that induced by ba. cattle were exposed to ye 0:9 by the oral or intramammary routes. oral exposure failed to generate significant serologic response. in contra ... | 1985 | 2422134 |
| antigenic differences between brucella abortus and brucella melitensis recognized by a monoclonal antibody. | by means of somatic hybridization of spleen cells from balb/c mice inoculated with brucella abortus and of a line of murine myeloma, a monoclone was obtained producing an antibody which specifically recognizes an epitope of brucella abortus by means of the agglutination reaction. this epitope is not only on the surface of the homologous strains but on all the brucella abortus biotype i, on the brucella melitensis biotype ii and on the brucella suis biotype ii tested. on the contrary it is not pr ... | 1986 | 2423851 |
| localization of antigen-specific lymphocytes following lymph node challenge. | the effect of subcutaneous injections of brucella abortus strain 19 antigen on the specific localization of autologous lymphocytes in the regional nodes of calves was analysed by fluorescent labelling and flow cytometry. both in vitro and in vivo fitc labelling of lymphocytes indicated the preferential migration of lymphocytes from a previously challenged lymph node to a recently challenged lymph node. however, lymphocytes from a lymph node challenged with b. abortus failed to localize preferent ... | 1986 | 2426183 |
| tma-specific first-order t-suppressor hybridoma. i. characterization of the hybridoma-derived single-chain inducer suppressor factor, tsf1. | experiments described in this report will characterize a monoclonal phenyltrimethylammonium (tma) specific, first-order t-suppressor factor (tsf1) produced by a t-cell hybridoma, 8a.3. the hybridoma expressed the thy-1, lyt-1, lyt-2 antigens as well as cross-reactive idiotypic (cri) determinants but did not express i-j encoded epitopes. it was also found to bear determinants recognized by a monoclonal antibody raised against single-chain gat-specific tsf1. the hybridoma-derived factor was capabl ... | 1986 | 2427221 |
| purification of nonlipopolysaccharide antigen from brucella abortus during preparation of antigen used for indirect hemolysis test. | the indirect hemolysis test (ihlt) for the diagnosis of brucellosis uses a lipopolysaccharide (lps) antigen obtained by dimethyl sulfoxide extraction of brucella abortus. we showed that a non-lps antigen can be obtained as a by-product of the ihlt antigen preparation. the antigen was purified to homogeneity by a combination of gel-filtration chromatography and ion-exchange chromatography. the substance contained 8% protein and about 65% carbohydrate. the molecular weight of the primary unit was ... | 1986 | 2429988 |
| brucella outer membrane lipoprotein shares antigenic determinants with escherichia coli braun lipoprotein and is exposed on the cell surface. | in an enzyme-linked immunosorbent assay (elisa), purified brucella abortus and escherichia coli peptidoglycan-linked lipoproteins gave a strong cross-reaction with sera from rabbits hyperimmunized with the heterologous lipoprotein. when smooth e. coli cells were used as elisa antigens, the immunological cross-reaction was not observed unless the cells were treated to remove lipopolysaccharide and other outer membrane components. in contrast, intact cells from smooth strains of b. abortus and bru ... | 1987 | 2432014 |
| influence of the macromolecular form of a b cell epitope on the expression of antibody variable and constant region structure. | we investigated the influence of the macromolecular form of an epitope on the structure of antibody variable and constant regions expressed by the b cell population participating in an immune response to that epitope. hybridomas were constructed from strain a/j mice undergoing either primary or secondary immune responses to p-azophenylarsonate conjugated to brucella abortus (ars-bruc). we determined the sequences of the v genes expressed by hybridomas selected on the basis of expression of a sin ... | 1987 | 2442288 |
| electrophoretic and immunochemical study of the lipopolysaccharides produced by chemostat-grown escherichia coli o157. | two chemically different o-polysaccharides, a low molecular mass form of lps and core lps produced by chemostat-grown e. coli o157, were analysed by sds-page, silver staining and immunoblotting. the reactivities of the different o-polysaccharides with antisera prepared against e. coli o157 grown in batch culture, salmonella o30 or brucella abortus were very similar, showing that the o-polysaccharides share at least some antigenic determinants. the reactions of the low molecular mass lps with the ... | 1987 | 2452229 |
| defective b-cell tolerance in new zealand black mice. fc receptor-independence of resistance to low-epitope-density tolerogens. | trinitrophenyl (tnp) human gamma-globulin with low-epitope-density tolerizes b cells from normal bdf1 mice in a fc gamma receptor-dependent manner but does not tolerize b cells from preautoimmune nzb mice. in order to investigate the relationships between tolerance induction and epitope density independently of fc gamma receptor function in these two strains, tnp conjugates of two additional thymic-independent tolerogenic carriers, d-glutamic acid-d-lysine (d-gl) and carboxymethyl cellulose (cmc ... | 1988 | 2452699 |
| purified native haptens of brucella abortus b19 and b. melitensis 16m reveal the lipopolysaccharide origin of the antigens. | purification of the brucella polysaccharide referred to as native hapten (nh) and extracted from cells by the autoclaving procedure, was accomplished by ultrafiltration, followed by repetitive gel filtration using high-performance liquid chromatography on a "tsk-g2000-sw" column. the purified nh was analysed by sds-page, gas-liquid chromatography mass spectroscopy, and 13c and 1h nmr spectroscopy. nh from b. abortus b19 (nh-a) was shown to have a structure identical to that of a polysaccharide f ... | 1988 | 2460114 |
| activation events in hapten-specific b cells from tolerant mice. | we have studied hapten-binding cells from the spleens of normal and tolerant adult mice in terms of their ability to enlarge, proliferate, and differentiate into antibody-secreting cells. tolerant b cells showed clear defects in intermediate activation events in addition to a deficit in antibody-secreting cells. in these studies, isolated b cells were stimulated by t cell independent ag with lymphokines, or with mitogens, in the absence of filler cells. the number of antibody-secreting cells gen ... | 1988 | 2460526 |
| enzyme-linked immunosorbent assay for differentiation of the antibody response of cattle naturally infected with brucella abortus or vaccinated with strain 19. | purified o chain of brucella abortus was passively attached to polystyrene to differentiate antibody responses of cattle vaccinated with b abortus strain 19 from those of naturally infected cattle. in the indirect assay, using o polysaccharide as antigen, a single serum dilution was used and mouse monoclonal antibody to bovine l chain conjugated with horseradish peroxidase was the detection reagent. measurable antibody was not found in sera of vaccinated cattle, except for 3 sera from cattle tha ... | 1989 | 2465711 |
| evaluation of three lps-specific monoclonal antibodies for the immunodiagnosis of bovine brucellosis. | bovine sera collected during the australian brucellosis eradication campaign were used to assess the value of three monoclonal antibodies (mab bruce 1, 4 and 7) for the immunodiagnosis of bovine brucellosis in a competitive enzyme immunoassay (ceia). each mab reacted to a different epitope of lipopolysaccharide molecules on the cell surface of brucella abortus. when the sensitivity of the ceia was set at 100 per cent so that all infected animals were identified, the specificity of the test using ... | 1989 | 2472660 |
| definition of brucella a and m epitopes by monoclonal typing reagents and synthetic oligosaccharides. | the paradigm that brucella a and m epitopes are simultaneously expressed on single cells and within one antigen molecule was reinvestigated by using polysaccharide-specific murine monoclonal antibodies. monoclonal antibodies were generated to the m antigen of brucella melitensis 16m. chemically defined lipopolysaccharides and o polysaccharides from brucella abortus 1119-3, b. melitensis 16m, and yersinia enterocolitica o:9 were used to dissect the binding profiles of the b. melitensis antibodies ... | 1989 | 2474505 |
| protection of mice against brucella abortus infection by inoculation with monoclonal antibodies recognizing brucella o-antigen. | monoclonal antibodies recognizing the o-polysaccharide portion of brucella abortus strain 2308 provided balb/c mice with passive protection against challenge exposure with the homologous strain. numbers of colony-forming organisms in the spleen were reduced by igm and igg monoclonal antibodies. active immunization of mice, using b abortus 2308s lipopolysaccharide, resulted in production of igm antibody at 14 days. clearance of organisms in the actively immunized mice after challenge exposure at ... | 1989 | 2481994 |
| experimental infection of dogs with brucella abortus: effect of exposure dose on serologic responses and comparison of culture methods. | two studies of experimentally induced brucella abortus infections in dogs are reported. twenty dogs in experiment 1 were fed approximately 4.8 x 10(10) colony forming units of b. abortus strain 2308 (ba 2308), and 11 dogs in experiment 2 were fed approximately 3.4 x 10(14) ba 2308. serum samples from each infected dog and noninfected control were tested on the day of infection and at weekly and biweekly intervals post-infection (pi) for antibodies to b. abortus. the standard tube agglutination ( ... | 1989 | 2492215 |
| thyrotropin: an endogenous regulator of the in vitro immune response. | we have previously shown that thyrotropin (tsh), which is produced by lymphocytes in response to the t cell mitogen staphylococcal enterotoxin a, enhances in vitro antibody production to t cell-dependent and independent ag (srbc and trinitrophenylated brucella abortus [ba-tpn], respectively) as determined by a direct plaque-forming cell assay. as a result of these studies, experiments were designed to examine the possible immunoregulatory function of thyrotropin-releasing hormone (trh) on the in ... | 1989 | 2492328 |
| nucleotide sequence of the 16s rrna from brucella abortus. | | 1989 | 2493635 |
| brucella abortus regulates bovine macrophage-t-cell interaction by major histocompatibility complex class ii and interleukin-1 expression. | t-cell activation is dependent on nominal antigen associated with major histocompatibility complex (mhc) class ii molecules and interleukin-1 (il-1), both provided by antigen-presenting cells. we have studied the effects of brucella abortus and recombinant bovine gamma interferon (ifn-gamma) on bovine macrophage expression of mhc class ii and il-1 molecules and subsequent t-cell proliferation in response to b. abortus. when peripheral blood mononuclear cells were cocultured with b. abortus and i ... | 1989 | 2494112 |
| chemical and protective properties of brucella lipopolysaccharide obtained by butanol extraction. | lipopolysaccharide (lps) fractions were obtained from smooth cultures of brucella abortus strains 2308 and s-19 by butanol extraction procedures. the lps from the initial butanol extraction contained 10 to 15% protein and was reduced to less than 1% protein by treatment with proteinase k. the lps fractions were identified and characterized on the basis of the chemical analysis, sodium dodecyl sulfate gel electrophoresis, cesium chloride gradients, electron microscopy, and gel immunodiffusion. re ... | 1989 | 2494911 |
| duration of strain 2308 infection and immunogenicity of brucella abortus lipopolysaccharide in five strains of mice. | a study was conducted to compare immunogenicity of a brucella abortus lipopolysaccharide (lps) and the duration of infection in 5 strains of mice. mice of strains cba/nj, balb/c, cd-1, c3h/hen, and c3h/hej were allotted into 2 large groups (vaccinated with proteinase k-treated lps or nonvaccinated) and 6 subgroups based on the intervals between challenge exposure to b abortus strain 2308 and the week the response data were obtained. criteria used in comparing responses between the various strain ... | 1989 | 2494912 |
| immunologic, histopathologic, and bacteriologic responses of five strains of mice to brucella abortus strain 2308. | a study was conducted to establish baseline data on brucella abortus infection induced in 5 strains of mice (cba/nj, balb/c, cd-1, c3h/hen, and c3h/hej). the strains were compared on the basis of immunologic, histopathologic, and bacteriologic responses. there were 4 treatment groups for each strain of mice: (1) vaccinated with homologous lipopolysaccharide and challenge exposed to b abortus strain 2308; (2) not vaccinated but challenge exposed; (3) vaccinated and not challenge exposed; and (4) ... | 1989 | 2494913 |
| immunity to brucella abortus induced in mice by popliteal lymph node restricted strain 19 vaccination. | living vaccines induce immunity by lymphoid organs colonization that persists for a while before subsiding. brucella abortus 19 vaccine injected into the footpad of mice was shown to colonize the popliteal lymph node and the spleen for at least 15 days before progressive disappearance. administration of an immune serum before footpad vaccination restricted the colonization to the popliteal lymph node. the serum did not interfere with immunity when this immunity was measured by the virulent chall ... | 1989 | 2494933 |
| dot-enzyme linked immunosorbent assay for the detection of antibodies in bovine brucellosis. | a dot-enzyme linked immunosorbent assay (dot-elisa) for the detection of antibodies in bovine brucellosis using soluble antigens extracted from brucella abortus s-99 is described in which antigen was deposited on a nitrocellulose sheet (0.5 x 0.5 cm) bound to a plastic strip and the unsaturated sites blocked by a solution of spray-dried milk powder. sera were tested at dilutions of 1:800 and 1:1600, using rabbit anti-bovine-immunoglobulin or protein-a coupled to peroxidase as conjugates and diam ... | 1989 | 2495563 |
| bovine brucellosis: evaluation of field sera by a competitive and superimposable elisa utilising a monoclonal antibody against brucella abortus lipopolysaccharide. | with the aid of a horseradish peroxidase (hrp) tagged monoclonal antibody against smooth lipopolysaccharide from brucella abortus (bruce 1), a competitive and superimposable elisa test procedure for bovine brucellosis has been evaluated for its ability to discriminate between strain 19-vaccinated (s19-vacc) and biotype 1-infected (b1-inf) cattle. in the competitive assay, all sera from s19-vacc animals competed effectively against hrp-bruce 1 (low hrp activity), while 10 out of 40 b1-inf animals ... | 1989 | 2495700 |
| brucellosis in the gezira area, central sudan. | twenty-nine patients presenting with symptoms and signs suggestive of brucellosis in the gezira area, central sudan, were studied. the majority of the patients (76%) were found to have a combined infection of both brucella abortus and brucella melitensis with titres of 1/160 and above. the social habit of eating raw meat, e.g. raw liver or other offal with spices (marrara or umfitfit) is found to be an important epidemiological factor in contracting the disease. raw milk consumption, since it is ... | 1989 | 2496237 |
| macrophage function in mammary glands of brucella abortus-infected cows and cows that resisted infection after inoculation of brucella abortus. | nonvaccinated pregnant cows were segregated retrospectively into 2 groups following inoculation with brucella abortus strain 2308. one group resisted infection (resistant cows) and the other group developed active infections (susceptible cows) and subsequently aborted. mammary gland macrophages collected from the 2 groups of cows were compared, using in vitro functional assays. in a chemiluminescence assay, mammary gland macrophages from resistant cows produced significantly (p = 0.014) higher o ... | 1989 | 2496628 |
| total lymphoid irradiation reduces igg autoantibody production and enhances specific antibody responses in nzb/nzw f1 mice. | thymus-independent primary antibody responses were studied in young and old (9 months) untreated and tli-treated nzb/nzw and balb/c mice. untreated old nzb/nzw mice had a low primary response to brucella abortus (ba) as compared to that of young nzb/nzw and balb/c mice. however, tli treatment resulted in a 130-fold increase in the igg anti-ba primary antibody response at day 21 postimmunization, achieving similar levels to those of young nzb/nzw or nonautoimmune balb/c mice. anti-tnp responses t ... | 1989 | 2500257 |
| immunogenicity of brucella abortus salt-extractable proteins. | the immunogenic properties of salt-extractable proteins and chromatographic fractions thereof from brucella abortus were evaluated in lemmings (dicrostonyx rubricatus). the efficacy of the brucella proteins as immunogens was determined after challenge with virulent b. abortus strain 2308 and was based on protection against clinical signs and gross lesions of brucellosis, as well as on numbers of viable brucella in the spleen. vaccination of lemmings with as little as 0.1 microgram of salt-extrac ... | 1989 | 2503922 |
| identification of a virulence factor for brucella abortus infection in balb/c mice. | immunogenic or pathogenic factors of recombinant proteins (rbcsp20, rbcsp-31, and rbcsp45 of brucella abortus strain 19) for mice were compared with factors of a proteinase k-treated lipopolysaccharide extracted from b abortus strain 2308. mice were vaccinated with 4 products, using different inoculation schedules and were challenge exposed with a virulent culture of b abortus strain 2308. blood samples were collected 2 weeks after vaccination and at necropsy and sera were obtained. spleens were ... | 1989 | 2504086 |
| properties and partial genetic characterization of nepean phage and other lytic phages of brucella species. | nepean (np), a new brucellaphage, was associated with atypical brucella abortus strains from ontario cattle. carriage of np was associated with loss of smooth lipopolysaccharide, changes in some protein bands in acrylamide gel electrophoresis profiles, increased susceptibility to colistin, and increased resistance to ultraviolet killing. nepean (np) was compared with brucellaphages tb, fi, wb, iz and r/c. all were morphologically identical, with icosahedral capsids (50-65 nm diameter) and short ... | 1989 | 2504475 |
| plasmid transfer and plasmid-mediated genetic exchange in brucella abortus. | naturally-occurring plasmids and gene transfer mechanisms have not yet been reported in brucellae. here we show that brucella abortus is capable of maintaining and transferring the broad-host-range plasmids pth10 (incp), psa (incw) and r751 (incp), and describe pth10-mediated transfer of b. abortus chromosomal genes to escherichia coli. all three plasmids transferred by conjugation from e. coli to b. abortus s19, and from b. abortus s19 to b. abortus 292 (biovar 4). they were stably maintained w ... | 1989 | 2504476 |
| efficacy of various treatment regimens, using liposomal streptomycin in cows with brucellosis. | thirty cows naturally infected with brucella abortus were treated by various routes, using free or liposomal streptomycin or a combination of liposomal streptomycin and a long-acting oxytetracycline preparation. of 21 cows treated with liposomal streptomycin alone, 3 (14%) were culture negative and 3 had 10 or fewer bacterial colonies isolated from tissues obtained at necropsy. thirteen (62%) cows continued to shed organisms in udder secretions and were considered treatment failures. of 9 cows t ... | 1989 | 2505648 |
| a farming systems study of abortion in dairy cattle on the atherton tableland. 2. the pattern of infectious diseases. | the role of infectious agents on dairy farms on the atherton tableland in tropical north queensland was studied as part of a comprehensive investigation into the causes of bovine abortion. the prevalence of antibody in serums collected from 7 herds whose annual abortion rates ranged from 3% to 21% were as follows: leptospira hardjo 49.9% (426/853), l. pomona 0.4% (3/851), bovine virus diarrhoea (bvd) 33.7% (35/104). infectious bovine rhinotracheitis virus (ibr) 11.5% (12/105), akabane virus 92.2 ... | 1989 | 2505741 |
| the effect of harderian adenectomy on the antibody response in chickens. | intact chicks and those that had their glands of harder (gh) removed (ghx) at 1 day of age were studied for their response to optically or intraperitoneally (ip) applied antigens. following exposure of the chicks to sheep red blood cells (srbcs), killed brucella abortus, or bovine serum albumin (bsa), serum and tear samples were collected and assayed for antibodies. of the two sources of antibodies, the serum generally had higher levels than did the tears. the only exception to this occurred in ... | 1989 | 2505743 |
| arthropathy associated with brucella abortus strain 19 vaccination in cattle. i. examination of field cases. | thirty cows presenting with lameness and persistent serological reactions to brucella abortus had chronic granulomatous arthropathy of the femorotibial and occasionally other joints. attempts to culture brucella or other pathogens gave negative results but organisms of brucella morphology were seen in fluorescent antibody-stained cryostat sections of synovial tissue. the synovial fluids contained high titres of antibodies to b. abortus and yersinia enterocolitica o:9 and had elevated total prote ... | 1989 | 2505883 |
| arthropathy associated with brucella abortus strain 19 vaccination in cattle. ii. experimental studies. | attempts to reproduce in calves the arthropathy associated with brucella abortus strain 19 (s19) vaccination by direct intra-articular injection of s19 or virulent brucella cells were unsuccessful. intra-articular injection of rabbits and a calf with immune complexes isolated from the synovial fluid of a field case produced clinical and histological signs of arthropathy accompanied by the development of rheumatoid factor. | 1989 | 2505884 |
| brucellosis of camels in kuwait. | this study investigated the presence of brucella antibodies in serum and milk obtained from camels in kuwait. brucella strains were also isolated from the foetus using standard technique (webridge lab techniques). three serological tests for serum were adopted. these tests were rose bengal plate test (rbpt), the serum tube agglutination test (sat) and the complement fixation test (cft). the rbpt was used for all sera samples, and both sat and cet were used for the positive rbpt. camels that show ... | 1989 | 2505964 |
| comparison of dot enzyme-linked immunosorbent assay (dot-elisa) with other serological tests for the detection of brucella antibodies in bovine sera. | a dot enzyme-linked immunosorbent assay (dot-elisa), using whole cell brucella abortus antigen dotted on the nitrocellulose membrane bound to a plastic strip (dipstick) was employed for the detection of brucella antibodies in bovine sera. the results were compared with that of serum agglutination (sat), rose bengal plate agglutination (rbpt) and complement fixation test (cft). all the four tests gave negative reaction in 127 sera obtained from a brucellosis free herd. testing of 549 sera from a ... | 1989 | 2506717 |
| comparison of protein components of different species and strains of brucella. | the proteins constituents of brucella abortus, brucella melitensis and brucella ovis were analyzed sds-page. from the comparison appears that the three species of brucella studied shows a different electrophoretic pattern specially at the level of small peptides. on the contrary when two strains of b. abortus are analyzed no differences can be noticed. | 1989 | 2506718 |
| uptake and excretion of brucella abortus in tissues of the face fly (musca autumnalis). | to determine their capacity to host brucella abortus, face flies were examined 1 to 120 hours after feeding on broth containing bacteria and bovine erythrocytes. brucella abortus was cultured in large numbers from whole flies for 12 hours after feeding, but not after 72 hours. histologic analysis showed that brucellae were rapidly taken into the midgut, sequestered from erythrocytes, transiently stored, and shed in the feces; there was no evidence of bacterial replication within epithelial cells ... | 1989 | 2506781 |
| development of the peripheral immune function in the chicken. a study on the bursa of fabricius isolated from the rest of the gut-associated lymphoid tissue (galt). | the bursa of fabricius was surgically separated from the rest of the gut-associated lymphoid tissue (galt). the antigens, sheep red blood cells (srbc) and killed brucella abortus organisms, were introduced into the bursal lumen, into the gut only, or allowed to get in contact with both (controls). the antibody titres in serum, the development of the bursal weight and the bursal histology were studied at various ages. the control birds responded vigorously to both antigens at between 2 and 8 week ... | 1989 | 2506917 |
| effects of antigen and antigen concentration on serum antibody titres in chickens primed per bursam. | a novel method for intrabursal immunization was applied to study the priming effect in 2-day-old and 4-week-old chickens. this immunization method separates the bursa of fabricius totally from the rest of the gut-associated lymphoid tissue (galt), and closely mimics the natural way antigens come in contact with the bursal epithelium. sheep red blood cells (srbc) and brucella abortus organisms in 4 different concentrations were used as antigens. in 4-week-old birds, a suppressive effect of per bu ... | 1989 | 2506918 |
| use of dried blood on filter paper in the elisa for brucella abortus. | an elisa for brucella abortus antibody detection using blood collected on filter paper is described. the method gave similar results to the complement fixation test. the signal-noise ratio was good. the system offers considerable advantages when transport of serum samples to the laboratory causes problems. | 1989 | 2507643 |
| bovine monoclonal antibody specific for brucella abortus lipopolysaccharide. | the development of a bovine monoclonal antibody against brucella abortus smooth lipopolysaccharide (bm-8) by interspecies fusion of bovine peripheral lymphocytes from an immunized cow and a murine plasmacytoma cell line is described. the twice cloned cell line secreted bovine igg1 subclass antibody. ascites fluid was prepared in pristane treated nu/nu mice by intraperitoneal injection. the pooled ascites fluid was purified by affinity chromatography and the functions of the antibody assessed in ... | 1989 | 2508298 |
| dna sequence and expression of the 36-kilodalton outer membrane protein gene of brucella abortus. | the cloning of the gene(s) encoding a 36-kilodalton (kda) cell envelope protein of brucella abortus has been previously described (t. a. ficht, s. w. bearden, b. a. sowa, and l. g. adams, infect, immun. 56:2036-2046, 1988). in an attempt to define the nature of the previously described duplication at this locus we have sequenced 3,500 base pairs of genomic dna encompassing this region. the duplication represented two similar open reading frames which shared more than 85% homology at the nucleoti ... | 1989 | 2509359 |
| capacity of passively administered antibody to prevent establishment of brucella abortus infection in mice. | in contrast to immunity against some other facultative intracellular parasites, protective immunity against brucella abortus is mediated in mice by antibodies as well as by cell-mediated immune responses. it was the purpose of this study to determine whether antibody alone would prevent infection with b. abortus. the majority (82%) of cd-1 outbred mice infected with 100 cfu of virulent b. abortus 2308 preincubated with graded quantities of an o polysaccharide-specific igg2a monoclonal antibody ( ... | 1989 | 2509362 |
| temporal development of protective cell-mediated and humoral immunity in balb/c mice infected with brucella abortus. | in balb/c mice infected i.v. with attenuated strain 19 of brucella abortus, the organism replicates to high numbers in the spleen and reaches peak concentrations at 2 wk postinfection (p.i.). the infection is then progressively cleared so that by 8 wk p.i. numbers of bacteria have decreased 10,000 fold or more. passive transfer assays were performed with t cell-enriched spleen cells and serum of donor mice infected 2, 3, 4, 5, 6, or 8 wk previously. antibodies conferred significant protection to ... | 1989 | 2509555 |
| polycarbonate membranes: a novel surface for solid-phase determinations with utility in field format serological assays. | a dipstick suitable for immunoassay procedures has been developed, and its utility demonstrated by the development of tests for the determination of immunoglobulin levels in newborn bovine blood, and for the detection of bovine antibodies against brucella abortus. the dipstick is of simple design, consisting of three components: a polycarbonate membrane, an adhesive, and a support material. the polycarbonate membrane is a porous filter material upon which the immunoassay is undertaken. biomolecu ... | 1989 | 2509564 |
| pathogenesis of brucella abortus infection of the mammary gland and supramammary lymph node of the goat. | goats, both in late pregnancy and soon after parturition, were inoculated intravenously with brucella abortus, and mammary glands and supramammary lymph nodes were examined by light and electron microscopy at 2 to 55 days post-inoculation. after 7 days, lymphoplasmacytic, histiocytic interstitial mastitis with a lobular and periductal distribution were detected microscopically. brucellae, identified in tissues with immunoperoxidase staining and antibody-coated colloidal gold stain, were first se ... | 1989 | 2511656 |
| effect of nursing on brucella abortus infection of mammary glands of goats. | eight 1-year-old, goats were inoculated intravenously with brucella abortus (b. abortus) on the day of parturition and necropsied at 28 days after inoculation. four nursed their kids and four did not (milk was not removed from the udders). tissues and fluids were examined by bacterial isolation, light microscopy, and serologic methods. nonnursing goats had high titers of brucellae (less than or equal to 10(8) organisms/ml) in milk (brucellae were isolated from four of four udders), had marked en ... | 1989 | 2511657 |
| intracellular localization of brucella abortus in bovine placenta. | | 1989 | 2513676 |
| brucella abortus-specific immunoglobulin isotypes in serum and vaginal mucus from heifers vaccinated with brucella abortus salt-extractable proteins and challenge exposed with virulent brucella organisms. | serum and vaginal brucella-specific immunoglobulin isotypes (igg1, igg2, igm, and iga), obtained from 62 crossbred beef heifers vaccinated with brucella abortus salt-extractable proteins and subsequently challenge exposed with b abortus s2308, were studied. brucella-specific igg antibodies and brucella-specific immunoglobulin isotypes were quantitated by a fluorometric immunoassay. serum and vaginal immunoglobulin responses were evaluated as a method of distinguishing infected from noninfected h ... | 1989 | 2514615 |
| detection of brucella abortus in mammalian tissue, using biotinylated, whole genomic dna as a molecular probe. | a method has been developed for the detection of brucella abortus in complex tissue homogenates. the technique uses tissue homogenization in the presence of sucrose and triton x-100 and subsequent filtration through a 5-microns pore size filter to remove mammalian nuclei and cellular debris. the dna from the bacteria is then extracted, dot blotted onto nitrocellulose, and hybridized with a biotinylated probe of b abortus strain 19 dna. in the present study, balb/c mice were inoculated intraperit ... | 1989 | 2514617 |
| interference with the humoral immune response in diverse genetic lines of chickens. ii. the effect of colloidal carbon. | two experiments were conducted to study the difference in humoral immune responses between lines of chickens selected for high (h) or low (l) antibody production to sheep red blood cells (srbc). prior to i.v. immunization with srbc or brucella abortus (ba), chicks of both lines were injected with either 2, 3 or 4 ml carbon suspension (59 mg carbon/ml) per kg body weight; controls were not injected. in both the h and l line, a higher dose of carbon showed a more progressive depression of the tota ... | 1989 | 2515649 |
| evaluation of humoral immunity to brucella sp in cattle by use of an agar-gel immunodiffusion test containing a polysaccharide antigen. | results of a double agar gel immunodiffusion (ouchterlony) test that contained a polysaccharide (poly-b) antigen of brucella melitensis strain b115 were compared with those of 5 other serotests. to determine the sensitivity and specificity of the immunodiffusion, standard tube, 2-mercaptoethanol, rivanol, card, and complement fixation tests, sera obtained from 1,328 vaccinated, infected, and seronegative cattle, 56 of which had been examined bacteriologically, were used to evaluate the humoral r ... | 1989 | 2515779 |
| isolation of brucella abortus from experimentally infected dromedary camels in sudan: a preliminary report. | six camels were experimentally infected with two strains of brucella abortus, four with s19 and two with a field bovine strain. in all cases antibody titres were detected within 6 to 11 days. serum agglutination titres peaked between days 11 and 32 and complement fixation titres between days 11 and 52; both titres then declined steadily. no clinical signs were observed in the four camels inoculated with s19. slight non-specific symptoms were seen in the two camels infected with the field bovine ... | 1989 | 2517155 |
| comparison of three culture techniques for the isolation of brucella abortus from bovine supramammary lymph nodes. | three different culturing techniques were compared and evaluated to determine the most effective method for isolating brucella abortus from bovine supramammary lymph nodes (sm's). in method i, the sm was sliced in half, and the inner surface was minced finely with a sterile scalpel. the minced surface was spread onto the agar surface of 4 selective media. in method ii, the sm was cut into small pieces and placed in a bag with a volume of phosphate-buffered saline equal to the volume of the lymph ... | 1989 | 2518695 |
| studies on the antigenic s-type lipopolysaccharides of brucella abortus strains 7 and mustapha. | antigenic phenol phase s-type lipopolysaccharides (lps) isolated from brucella abortus (b. abortus) strains 7 and mustapha were observed to have 13c n.m.r. spectra which were almost identical to the one reported for the brucella abortus 1119-3. the glycosyl content of the lipid a obtained from the lps of strain 7 was found to be 2-acetamido-2-deoxyglucose only while strain mustapha was found to contain both 2-acetamido-2-deoxyglucose and 2-acetamido-2-deoxygalactose. the fatty acid present in th ... | 1989 | 2518732 |
| [human infection caused by brucella abortus]. | | 1989 | 2518768 |
| immune dysfunction associated with graft-vs-host reaction in mice transplanted across minor histocompatibility barriers. ii. reversible defect in t-dependent antibody responses. | b cell and th cell functions were assessed in mice undergoing a graft-vs-host reaction (gvhr) in response to minor histocompatibility ag by using the plaque-forming cell (pfc) response to the t-independent ag tnp-brucella abortus and the t-dependent ag tnp-srbc. bone marrow plus spleen cells from b10.d2 mice were transplanted into lethally irradiated b10.d2 (syngeneic recipient) or h-2d-compatible balb/c (allogeneic recipient) to produce a chronic form of gvhr. balb/c recipients of an allogeneic ... | 1989 | 2523932 |
| stimulatory effect of avian influenza virus on chicken lymphocytes. | a study was conducted to examine the effect of avian influenza virus (aiv) on chicken lymphocyte activation. unprimed or brucella abortus antigen (ag)-primed lymphocytes were incubated with various doses of the t-cell mitogen concanavalin a (con a) or ag, respectively, plus serial dilutions of inactivated aiv for 72 hr, and cell proliferation was measured via uptake of tritiated thymidine. aiv enhanced the proliferative response to con a or ag by 150% or better, and the enhancement decreased in ... | 1989 | 2533493 |
| comparative pathogenesis of serotype 1 and variant serotype 1 isolates of infectious bursal disease virus and their effect on humoral and cellular immune competence of specific-pathogen-free chickens. | specific-pathogen-free chickens inoculated with isolate va (variant a) or isolate im of infectious bursal disease virus (ibdv) were examined for mitogenic response to t-cell mitogens, primary and secondary antibody response to sheep erythrocytes and brucella abortus, and gross and histologic lesions in thymus and bursa. both isolates induced comparable depression in the mitogenic and antibody response, and both caused extensive gross and histologic lesions in the bursa of fabricius. however, bur ... | 1989 | 2539070 |
| effects of in vitro and in vivo administration of recombinant bovine interferon-gamma on bovine neutrophil responses to brucella abortus. | the effects of in vitro and in vivo treatment of bovine polymorphonuclear leukocytes with recombinant bovine interferon-gamma on in vitro bovine polymorphonuclear leukocyte functions and the survival of brucella abortus were determined. activation of neutrophils in vitro with interferon-gamma resulted in enhanced production of o2- and myelopeoroxidase-h2o2-halide activity by neutrophils in the presence of b. abortus. the improved iodination responses were correlated with an enhanced ability to p ... | 1989 | 2539694 |
| dna homology of brucella abortus strains 19 and 2308. | the restriction endonuclease digestion dna patterns from brucella abortus strains 19 and 2308 were examined with 11 restriction enzymes (avai, bamhi, bglii, bsteii, ddei, ecori, hindiii, kpni, psti, xbai, and sali). the dna electrophoretic banding patterns between the 2 strains were highly similar, using this restriction enzyme analysis. differences were not discernable between b abortus strains 19 and 2308 in any of the restriction banding patterns examined. methylation at ccgg or gatc sites wa ... | 1989 | 2543240 |
| sepracell-mn for mononuclear cell separation from cattle: effect on mitogen-and antigen-induced lymphocyte blastogenesis. | mononuclear leukocytes (mnc) were separated from heparinized and edta-treated whole bovine blood by centrifugation after mixing with a commercial colloidal silica preparation (sepracell-mn (s-mn]. cell yields and lymphocyte blast transformation (lbt) to pokeweed mitogen (pwm), phytohaemagglutinin (pha), concanavalin a (con a), and brucella abortus antigens were tested against mnc obtained from heparinized whole blood using ficoll-hypaque (fh). separation with s-mn was more rapid and less labor i ... | 1989 | 2557707 |
| the use of haptenated-immunoglobulin molecules to induce tolerance in b cells from neonatal mice. | the role of the fc region of trinitrophenylated (tnp)-immunoglobulins (ig) in their ability to induce tolerance in immature b cells was examined. with the use of b cells from neonatal mice, tolerogens that could or could not bind to fc receptors were assessed for their ability to induce tolerance. this was accomplished by tolerizing spleen cells in bulk culture and assessing the degree of tolerance by challenging the cells with the thymus-independent antigen tnp-brucella abortus (tnp-ba) in limi ... | 1985 | 2578508 |
| delayed maturation of the antibody response to type 2 thymus-independent antigens in a partially inbred strain of chicken. | the ontogeny of antibody responses to trinitrophenylated (tnp) thymus-independent (ti) antigens was compared in two partially inbred strains of chicken: the sc strain (b2/b2 genotype) and the fp strain (b15/b22 genotype). in the sc chicken, maturation of both the splenic anti-tnp plaque-forming cell (pfc) response and the 19s hemagglutinating antibody response to ti type 2 (ti-2) antigens, tnp-ficoll and tnp-dextran, were delayed to a significantly later time in ontogeny (20 wk of age) than in t ... | 1985 | 2579135 |
| immunoregulation in the rat: ontogeny of b cell responses to types 1, 2, and t-dependent antigens. | the development of rat b cells has been examined in neonatal and adult fischer rats through the use of type 1 (tnp-brucella abortus), type 2 (tnp-lps(ph), tnp-ficoll) and t cell-dependent (td) (srbc) antigens. in vivo splenic pfc responses to tnp-brucella abortus could be induced in newborn rats and by 12 days of age had reached adult levels. in contrast, the responses to the type 2 and td antigens were 30% and 70%, respectively, of the adult levels at 30 days of age. adoptive transfer of the b ... | 1985 | 2580000 |
| b cell activation: role of dendritic and t cell factors in the response to thymic-independent and -dependent antigens. | we described a cloned dendritic cell, clone den-1, that is a potent accessory cell for some b cell responses. clone den-1 produces a novel lymphokine that is distinct from previously described factors produced by t cells. in the present study, we compare the role of nonspecific helper factors produced by den-1 (den-1 sn) or the t cell thymoma el4 (el4-sn) in promotion of b cell plaque-forming cell (pfc) responses to a variety of antigens. we find that the antigen in culture determines the b cell ... | 1985 | 2580002 |
| monoclonal antibodies directed against brucella abortus cell surface antigens. | a panel of monoclonal antibodies (mab) has been raised against brucella abortus cell surface antigens from mice immunized with either heat/phenol treated or uv killed bacterial suspensions of b. abortus. the hybridomas were screened by either a microagglutination procedure or by an indirect enzyme immunoassay (eia) on sonicated bacterial preparations. from a large number of mab generated by various procedures, two distinct types of mab emerged. the most numerous type was capable of agglutinating ... | 1985 | 2581358 |
| [brucella abortus]. | | 1989 | 2595719 |
| cigarette smoke causes inhibition of the immune response to intratracheally administered antigens. | chronic inhalation of cigarette smoke in rats preferentially inhibited the plaque-forming cell (pfc) response of lung-associated lymph nodes (laln) to sheep red blood cells (srbc), compared to anatomically distant lymph nodes. inhibition of the antibody response in laln of smoke-exposed animals was first detected at 21 weeks of smoke inhalation and was well established by the 27th week of smoke exposure. after prolonged exposure (greater than 34 weeks) to cigarette smoke, similar smoke-induced c ... | 1989 | 2609346 |
| inflammatory cell and immune function in merino sheep with chronic dermatophilosis. | components of inflammatory and immunological responses were compared in 17 merino sheep with chronic dermatophilosis (group 1) and 15 merino sheep that had recovered from the disease (group 2). the functions studied included: (i) total and differential white cell counts; (ii) phagocytic function and intracellular killing by neutrophils; (iii) humoral immune response to t-dependent and t-independent antigens and to dermatophilus congolensis. (iv) lymphocyte blastogenic responses to phytohaemagglu ... | 1989 | 2623798 |
| comparison of the dot-immunobinding assay with the complement fixation test for the detection of brucella antibodies in sheep. | a dot-immunobinding assay (dia), using as antigen a sonic extract of brucella abortus dotted on nitrocellulose bound to a plastic strip, was employed for the detection of brucella antibodies in 666 sheep sera. the results were compared with the complement fixation test (cft). all the 242 sera belonging to two flocks were found to be negative by dia. cft was negative in 239 cases, whereas three samples showed anti-complementary activity. of the 424 sera from the remaining three flocks, 98 were po ... | 1989 | 2672550 |
| prevalence of hazardous exposures in veterinary practice. | all female graduates of a major u.s. veterinary school were surveyed by mailed questionnaire to obtain details of work practice and hazard exposure during the most recent year worked and during all pregnancies. exposure questions were based on previously implicated occupational hazards which included anesthetic gases, radiation, zoonoses, prostaglandins, vaccines, physical trauma, and pesticides. the response rate was 86% (462/537). we found that practice type and pregnancy status were major det ... | 1989 | 2750751 |
| monocyte locomotion in anergic chronic brucellosis patients: the in vivo effect of ascorbic acid. | in 14 patients suffering from relapsing chronic brucellosis who were anergic to brucella antigens, we have studied peripheral blood monocyte random migration and chemotaxis against non-specific and specific leukoattractants, as well as plasma and monocyte ascorbic acid levels. we found that all parameters studied, were significantly beneath normal, when compared to normal subjects. after the oral administration of ascorbic acid at a daily dose of 1gr for 15 consequetive days, random and directed ... | 1989 | 2760414 |
| effects of cyclopiazonic acid and aflatoxin singly and in combination on selected clinical, pathological and immunological responses of guinea pigs. | cyclopiazonic acid (cpa) and aflatoxin are known sometimes to coexist in nature but little is known of possible biological interaction in mammals that consume mixtures of these two mycotoxins. guinea pigs were dosed orally with cpa (2.2 mg/kg) or aflatoxin (0.045 mg b1/kg) singly or in combination. effects of toxin consumption were determined on clinical health, body weight gain, pathological change, and several immunologically related parameters including delayed cutaneous hypersensitivity, ant ... | 1989 | 2761608 |
| skin test reactions to a phenol-soluble antigen of brucella abortus among veterinary students, lyon, france, 1984. | in december 1984, 424 students at the national veterinary college, lyon, france, were skin-tested with a phenol-soluble antigen of brucella abortus b19. of all students, 2.6% had a positive intradermal reaction indicating previous contact with brucella. prevalence of positive reactions was significantly lower among students from the three first school years (less than 2%) compared with students in their last school year (5.9%). these results are discussed and compared to the prevalence of brucel ... | 1989 | 2767861 |
| evaluation of a delayed-type hypersensitivity test for the diagnosis of brucella abortus infection in cattle. | the delayed-type hypersensitivity (dth) test was used to diagnose brucellosis in two cows experimentally induced with brucellosis, and 176 dairy cows from a farm suspected of brucellosis. dth test results were compared with results of the milk ring test, the serum agglutination test, the complement fixation test and the coombs test. cows positive in the dth test and in one of the other tests were examined bacteriologically. in experimentally infected animals the dth test was positive 10 days aft ... | 1989 | 2773300 |
| [brucella clearance as a sensitive method for the detection of cross reactions of brucella abortus with yersinia enterocolitica 03, 06, 09 and salmonella urbana and salmonella abony]. | determination of the brucella clearance rate has proved to enable assessment of brucella immune reaction in rat, even after vaccination with yersiniae and salmonellae. vaccination with yersinia (y.) enterocolitica o6 and o9 produced 95 per cent of "high responders", whereas 65 per cent of "high responders" and 25 per cent of "non-responders" were recorded in the wake of o3. salmonella (s.) urbana vaccination gave 50 per cent of "high responders" and 27 per cent of "non-responders", while 100 per ... | 1989 | 2774825 |