| serological classification of achromobacter xylosoxidans. | adult rabbits were immunized with nine achromobacter xylosoxidans strains by intravenous injection of formalin-killed organisms. antisera thus obtained were reciprocally titrated with the nine a. xylosoxidans strains, and seven sera were defined as serologically distinct. three of nine antisera possessed one common antibody while also each having their own specific antibody. ninety-five strains of a. xylosoxidans were examined for serotyping by a microtiter agglutination test with the nine antis ... | 1983 | 6833473 |
| in vitro activity of azthreonam, a monobactam antibiotic. | we studied the activity of azthreonam (sq 26,776), a novel monocyclic beta-lactam compound, against a variety of clinical isolates. it was more potent than moxalactam, cefoperazone, cefamandole, cefoxitin, ticarcillin, tobramycin, or amikacin against strains of klebsiella spp., serratia spp., and the proteus group. it was highly effective against escherichia coli and strains of salmonella spp. the median minimal inhibitory concentration for all species of enterobacteriaceae was less than or equa ... | 1982 | 6891198 |
| infective endocarditis caused by actinobacillus actinomycetemcomitans and achromobacter xylosoxidans. | | 1982 | 6957084 |
| [two ways of formate oxidation in methylotrophic bacteria]. | the cells of achromobacter parvulus, strain 1t, when grown in a methanol-containing medium, have two formate dehydrogenases, i.e. nad-linked formate dehydrogenase and the formate dehydrogenase reducing the ferricyanide and tetrazolia. only the latter enzyme was found in the cells grown in a medium with glycerol as a carbon source. these enzymes differ with respect to km for formate and antigenic specificity. km for formate oxidation by the cells of a. parvulus is lower than for formate of the na ... | 1980 | 6246983 |
| achromobacter protease i-catalyzed conversion of porcine insulin into human insulin. | | 1980 | 6986867 |
| enterotoxigenic bacteria in food and water from an ethiopian community. | food and water samples from an ethiopian community were screened for the presence of enterotoxin-producing bacteria. using the chinese hamster ovary cell assay, 40 of 213 isolates (18.8%) produced heat-labile (lt) enterotoxin. these lt-producing isolates comprised 33 of 177 (18.6%) strains from 24 of 68 food samples (35.3%) and 7 of 36 (19.4%) isolates of 4 of 17 water samples (23.5%). one lt-producing strain each of salmonella emek and of shigella dysenteriae was found. three pseudomonads, all ... | 1981 | 7016032 |
| on the biosynthesis of biotin in achromobacter ivsw. a reinvestigation. | | 1981 | 7037003 |
| [study of the spermatic bacterial flora in infertile males (author's transl)]. | two groups of infertile males (65 and 132 patients) have been investigated in two different laboratories, with two different methods to obtain semen. the bacteriological results are quite similar in the two groups. the microorganisms which have been isolated are : beta-hemolytic streptococcus, proteus, epidermidis staphylococcus, micrococcus, corynebacter, viridans streptococcus, klebsiella, pseudomonas, enterobacter, bacillus, neisseria, escherichia coli, anaerobic staphylococcus, anaerobic str ... | 1982 | 7038597 |
| postoperative infection of an aortic prosthesis with achromobacter xylosoxidans. | | 1982 | 7064475 |
| the effects of temperature and ph on the growth of eight enteric and nine glucose non-fermenting species of gram-negative rods. | we studied the heat resistance and the range of growth temperature o gram-negative rods to find one of the bacterial factors governing their infectivity in exogenous and endogenous infections in predisposed patients. escherichia coli, klebsiella pneumoniae, serratia marcescens, pseudomonas aeruginosa, and acinetobacter calcoaceticus grew equally well at 25, 30, 37, and 42 c. among other sugar non-fermenting gram-negative rods, six species showed suppressed growth at either or both ends of the in ... | 1982 | 7087800 |
| [possible reason for growth inhibition of achromobacter cobalamini sp. nov. growing on molasses media]. | | 1982 | 7087806 |
| achromobacter pneumonia. | | 1982 | 7090381 |
| characterization of the major antigens of haemophilus equigenitalis (contagious equine metritis organism). | immunoelectrophoresis of ultrasonically disrupted haemophilus equigenitalis (contagious equine metritis organism) cells against rabbit and equine antisera disclosed at least 11 precipitating antigens. two of these, a polysaccharide and a lipopolysaccharide-protein complex, were of high molecular weight and located on the cell surface. the remaining antigens were intracellular and were small- to medium-sized proteins. the surface antigens were the most significant in relation to the serological r ... | 1982 | 7153516 |
| [effect of organic substances on the growth and cobalamin genesis of achromobacter cobalamini]. | corn steep and molasses were shown to stimulate cobalaminogenesis in achromobacter cobalamini. their concentrations optimal for the culture growth and vitamin b12 synthesis were found. corn steep at a concentration of 2% accelerated the growth. the biomass yield and the content of b12 were maximal after 48 h of fermentation. in the medium with molasses, the growth was stimulated at 1% of molasses, and the vitamin biosynthesis at 5%. when 5% of molasses was added into the mineral medium, the rate ... | 1982 | 7155004 |
| purification and properties of alpha-amino-epsilon-caprolactam racemase from achromobacter obae. | we have purified a unique enzyme, alpha-amino-epsilon-caprolactam racemase 945-fold from an extract of achromobacter obae by octyl-sepharose cl-4b and thiopropyl-sepharose 6b and some other chromatographies. the purified enzyme was found homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and analytical ultracentrifugation. the enzyme has a monomeric structure with mr approximately 50000 and a sedimentation coefficient (s20,w) of 4.28 s. the enzyme contains pyridoxal 5'-phos ... | 1982 | 7160482 |
| prosthetic valve endocarditis due to achromobacter xylosoxidans. | | 1981 | 7211679 |
| [the identification of nonfermentative gram-negative bacteria. experiences with 676 apyocyaninogenic strains (author's transl)]. | during a period of 16 months 1757 strains of nonfermentative gram-negative rods have been isolated from clinical material. of the, 1205 (69%) were p. aeruginosa, 124 (10%) of which failed to produce pyocyanin. the apyocyaninogenic strains as well as the remaining 552 isolates were differentiated by steps according to a diagnostic scheme developed by us. for identification of species two or three steps were needed. by this procedure, 530 of the 552 strains could be assigned to nineteen species wi ... | 1981 | 7223132 |
| pancreatic abscess associated with achromobacter group vd biovar 1. | a case of pancreatic abscess associated with achromobacter biovar 1 in a 75-year-old man with multiple predisposing debilitating conditions is presented. the infection responded to antibiotic therapy, but the patient died from unrelated causes. | 1980 | 7229011 |
| chemical modifications of achromobacter collagenase and their influence on the enzymic activity. | a study of the influence of chemical modifications on the activity of achromobacter iophagus collagenase (ec 3.4.24.8) has led to the following conclusions: a modification of 4 out of 80 cooh groups with carbodiimide led to 90% loss of enzymic activity. a 70% inactivation was found after modification of two tyrosines out of 30 with tetranitromethane. the modification of four to six tryptophans out of 16 with 2-hydroxy-5-nitrobenzyl bromide decreased enzyme activity to 36%. this inactivation is a ... | 1980 | 6251892 |
| morphological and biochemical differentiation of achromobacter and moraxella (debord's tribe mimeae). | to determine the most useful laboratory tests for the differentiation of achromobacter anitratus, achromobacter lwoffii, and moraxella duplex (debord's tribe mimeae), 157 strains of these bacteria, isolated from clinical specimens, were examined for their morphological and biochemical characteristics. there were several differences between these nonfermentative, gram-negative diplococci: moraxella was nonglucolytic in either infusion base or synthetic base, oxidase-positive, and sensitive to pen ... | 1968 | 5636469 |
| clinical and laboratory characteristics of achromobacter xylosoxidans infection. | achromobacter xylosoxidans was isolated from six patients. the organism causes opportunistic infections in patients who are compromised. a. xylosoxidans is a catalase- and oxidase-positive, motile, gram-negative rod that oxidizes xylose and glucose. the organism exists in a water environment and may be confused with pseudomonas species. unlike pseudomonas, achromobacter has peritrichous flagella. the clinical and laboratory characteristics of a. xylosoxidans are presented. | 1980 | 7358838 |
| [potential growth of achromobacter cobalamini on the wastes from hydrolysis production]. | | 1980 | 7412604 |
| [preservation of lyophilized cultures of saprophytic microorganisms]. | all of the tested saprophytic microbial cultures remained viable upon 11--16 years of storage in the lyophilized state at 3--6 degrees. the cultures belonged to the following taxonomic groups: acetobacter, achromobacter, azotobacter, bacillus, chromatium, escherichia, flavobacterium, lactobacillus, micrococcus, proteus, propionibacterium, pseudomonas, rhizobium, sarcina, serratia, streptococcus, torula, mycobacterium, actinomyces. the number of viable cells in the tubes decredased only slightly ... | 1980 | 7412623 |
| [infection in the dialysis unit as a contributing factor to the problem of hospital acquired infections]. | hospital acquired infections are especially dangerous for patients with chronic renal insufficiency. an epidemic of hospital acquired infection of dramatic course in described in 13 patients (8 being on haemodialysis (hd) and 5 treated conservatively), with 15 episodes of septic syndrome (12 cases) or septic shock (3 cases). initial cultures of blood, urine and pharyngeal swabs were positive only in 5 patients treated conservatively. initial cultures of dialysing fluid from all 12 monitors of hd ... | 1995 | 7479256 |
| variation in the fine structure of a marine achromobacter and a marine pseudomonad grown under selected nutritional and temperature regimes. | certain features of the fine structure of a marine achromobacter and a marine pseudomonad were dependent upon the conditions of growth. cells of achromobacter grown at 10 c in a low peptone-seawater (sw) medium displayed the characteristic morphology of the achromobacter: a regularly undulant outer element of the cell wall and a planar inner element, tightly packed ribonucleoprotein (rnp) particles in the cytoplasm, deoxyribonucleic acid (dna) disposed in a lobate manner, and dense inclusion bod ... | 1968 | 5650088 |
| [porphyrin and heme synthesis by achromobacter metalcaligenes under the influence of 2-methyl-2-n-propyl-1,3-propanedioldicarbamate]. | | 1968 | 5710906 |
| pyruvate metabolism by a nitrogen-fixing bacterium. | 1. the major products of pyruvate dissimilation by washed intact cells of achromobacter n4-b under nitrogen-fixing conditions are acetate and formate. the formation of succinate and isocitrate and the assimilated amino acids requires carbon dioxide fixation. 2. the products formed by cells incubated with pyruvate in an atmosphere of nitrogen were compared with those formed by cells incubated in an atmosphere of helium. production of hydrogen and the formation of succinate were greater under heli ... | 1965 | 5837784 |
| the metabolism of beta-phenylpropionic acid by an achromobacter. | 1. when a species of achromobacter grew with beta-phenylpropionate as carbon source, 2-hydroxy-beta-phenylpropionate and 2,3-dihydroxy-beta-phenylpropionate appeared in the growth medium. the concentrations of these compounds were maximal during exponential growth. 2. the cells contained an oxygenase that required fe(2+) ions and cleaved the benzene nucleus between the adjacent carbon atoms that bear the side chain and one hydroxyl group of 2,3-dihydroxy-beta-phenylpropionate. 3. the ring-fissio ... | 1965 | 5881653 |
| comparative effects of chlortetracycline, freezing, and gamma radiation on microbial population of ocean perch. | the microbial populations in chlortetracycline (ctc)-treated (50, 100, 200, and 500 ppm), frozen (-15 c), and irradiated (0.1 mrad) ocean perch (sebastodes alutus) were compared. the control sample spoiled at 7 c, primarily because of the growth of pseudomonas. irradiation changed this to achromobacter-dominated spoilage. freezing or ctc treatment altered the spoilage pattern very little. ctc was particularly effective against ultraviolet fluorescent pseudomonas species at the higher concentrati ... | 1967 | 6029834 |
| properties of pseudomonas enalia, a marine bacterium pathogenic for the invertebrate crassostrea gigas (thunberg). | bacteriological investigations of dead and dying oysters in populations of crassostrea gigas grown in hood canal, oyster bay, and willapa bay, washington, were undertaken. living, and presumably normal, oysters within the same sample set were also examined. results indicated that the natural flora of crassostrea gigas (thunberg) is composed of organisms representing the genera pseudomonas, achromobacter, flavobacterium, and vibrio. pollution indicator organisms such as escherichia coli were not ... | 1967 | 6053175 |
| [comparative susceptibility of ochrobactrum anthropi, agrobacterium tumefaciens, alcaligenes faecalis, alcaligenes denitrificans subsp. denitrificans, alcaligenes denitrificans subsp. xylosidans and bordetella bronchiseptica against 35 antibiotics including 17 beta-lactams]. | ochrobactrum anthropi, formerly known as "achromobacter sp." or cdc group vd has been isolated from water, hospital environment (antiseptic solutions, dialysis fluids ... ). o. anthropi is a gram negative, motile, strictly aerobic, oxydase positive and non-fermentative bacteria with a strong urease activity. the susceptibility of 13 strains of o. anthropi was determined by agar diffusion method and compared to those of type strains of agrobacterium tumefaciens, alcaligenes faecalis, alcaligenes ... | 1995 | 7567111 |
| studies on a new proteolytic enzyme from achromobacter lyticus m497-1. ii. specificity and inhibition studies of achromobacter protease i. | the unique specificity of achromobacter protease i for lysine residue was investigated using synthetic and natural substrates, i.e., lysine derivatives, arginine derivatives, lysine vasopressin, substance p, acth and insulin. the enzyme cleaved only the -lys-x- bonds in the above substrates. the binding affinity of alkylamines as determined by ki was much stronger than that of the corresponding alkylguanidines. | 1981 | 6168293 |
| screening and characterization of microorganisms with glutaryl-7adca acylase activity. | a screening of microorganisms producing glutaryl-7adca acylase, an enzyme able to hydrolyse glutaric acid selectively from glutaryl-3-deacetoxy-7-aminocephalosporanic acid (glutaryl-7adca), has been carried out in soil samples. five microorganisms expressing acylase activity were isolated and classified as bacillus cereus, achromobacter xylosooxidans, bacillus sp., pseudomonas sp. and pseudomonas paucimobilis. the screening was carried out by preparing enrichment cultures containing glutaryl-7-a ... | 1995 | 7632401 |
| [a case of suppurative skin disease which is thought to be due to achromobacter xylosoxidans (author's transl)]. | | 1980 | 6968842 |
| study of the role of arginine residues in bacterial formate dehydrogenase. | modification of 12 arginine residues per molecule of formate dehydrogenase (formate : nad+ oxidoreductase, ec 1.2.1.2.) from the methylotrophic bacterium, achromobacter parvulus i, by 2,3-butanedione results in complete inactivation of the enzyme. inactivation of the enzyme is reversible and proceeds in two steps via formation of the intermediate enzyme-butanedione complex. coenzymes but not formate effectively protect formate dehydrogenase from inactivation. complete maintenance of enzyme activ ... | 1981 | 7248314 |
| circular dichroism comparative studies of two bacterial collagenases and thermolysin. | the recently isolated and purified collagenase produced by achromobacter iophagus, the collagenase from clostridium histolyticum, and thermolysin, three enzymes having common properties, were studied by circular dichroism. from the spectra of the aqueous solutions obtained in the peptide region, the fraction of alpha helix, beta sheet and aperiodic segments in the three proteins could be estimated. good similarity was found between achromobacter collagenase and thermolysin, which both contain a ... | 1980 | 6250633 |
| immunosuppressive properties and circulating life of achromobacter glutaminase-asparaginase covalently attached to polyethylene glycol in man. | the immunosuppressive effects and circulating life of achromobacter glutaminase-asparaginase (ga) covalently attached to polyethylene glycol (peg) were examined in human subjects following a single iv dose of 1000 iu/m2. plasma half-life of peg-ga was 72 hours. skin test reactivity to recall antigens (mumps and tuberculin) was lost in all four patients tested. in vitro phytohemagglutinin-induced blastogenesis, "natural killing," and phytohemagglutinin-induced cell cytotoxicity was diminished as ... | 1981 | 7296553 |
| amino acid sequences of double-headed proteinase inhibitors from the seeds of canavalia lineata. | the amino acids of two bowman-birk type proteinase inhibitors (clti-i and -ii) from the seeds of canavalia lineata were sequenced by a manual edman degradation using the dabitc/pitc double coupling method after enzymatic digestions with achromobacter lyticus lysyl endopeptidase, staphylococcus aureus v8 protease, and chymotrypsin. clti-i contains 75 amino acid residues. clti-ii has an identical sequence to clti-i except an extra asp residue attached at the c-terminus. the inhibitors showed a hom ... | 1994 | 7764547 |
| property and amino acid sequence of a subtilisin inhibitor from seeds of beach canavalia (canavalia lineata). | a subtilisin inhibitor was purified from the seeds of canavalia lineata by ammonium sulfate precipitation, ultrafiltration on a ym-30 membrane, column chromatography on deae-toyopearl and sp-toyopearl, followed by reverse-phase hplc. the inhibitor (clsi-i) is a low molecular weight protein (m(r) about 6500) containing no half-cystine residue, and quite stable as to extreme heat and ph treatment. clsi-i inhibited subtilisin-type serine proteases including s. griseus alkaline protease. the amino a ... | 1994 | 7765594 |
| transfer and expression of pcb-degradative genes into heavy metal resistant alcaligenes eutrophus strains. | sites polluted with organic compounds frequently contain inorganic pollutants such as heavy metals. the latter might inhibit the biodegradation of the organics and impair bioremediation. chromosomally located polychlorinated biphenyl (pcb) catabolic genes of alcaligenes eutrophus a5, achromobacter sp. lbs1c1 and alcaligenes denitrificans jb1 were introduced into the heavy metal resistant alcaligenes eutrophus strain ch34 and related strains by means of natural conjugation. mobile elements contai ... | 1994 | 7765842 |
| amino acid sequencing, molecular cloning and modelling of the chick liver class-theta glutathione s-transferase cl1. | glutathione s-transferase cl1-2 heterodimers purified from 1-day-old chick livers were digested with achromobacter proteinase i. the resulting fragments were separated for amino acid sequence analysis. oligonucleotide probes were constructed based on sequence similarity to class-theta glutathione s-transferases for pcr using a chicken liver cdna library as template. a full-length clone (1725 bp) encoding a polypeptide comprising 261 amino acids was isolated. including conservative substitutions, ... | 1995 | 7492340 |
| mapping of the plasmid (plq3) from achromobacter and cloning of its cephalosporinase gene in escherichia coli. | we have constructed a physical map of the plasmid plq3 which was originally isolated from achromobacter and which codes for a beta-lactamase. the enzyme specified by plq3 is expressed in escherichia coli and is unusual in that it is a cephalosporinase, an enzyme usually coded for by chromosome. plasmid plq3 is 12.4 kb in length and has a unique bam hi site and two bglii sites. from a bamhi + bglii double digest of plq3, we have constructed a "shortened" plasmid, plq10, in which a 2.96-kb fragmen ... | 1982 | 6286419 |
| bacteremia caused by achromobacter species in an immunocompromised host. | a case of bacteremia caused by achromobacter species in an immunocompromised patient is described. the patient responded to antibiotic therapy. detailed antibiotic susceptibility data are presented. | 1984 | 6332118 |
| the structure of copper-nitrite reductase from achromobacter cycloclastes at five ph values, with no2- bound and with type ii copper depleted. | high resolution x-ray crystallographic structures of nitrite reductase from achromobacter cycloclastes, undertaken in order to understand the ph optimum of the reaction with nitrite, show that at ph 5.0, 5.4, 6.0, 6.2, and 6.8, no significant changes occur, other than in the occupancy of the type ii copper at the active site. an extensive network of hydrogen bonds, both within and between subunits of the trimer, maintains the rigidity of the protein structure. a water occupies a site approximate ... | 1995 | 7499203 |
| evidence for conformational change of fatty acid-binding protein accompanying binding of hydrophobic ligands. | conformational change of rat liver fatty acid-binding protein was investigated by making use of change of protease-susceptibility in the presence or absence of bound oleic acid and clofibrate. delipidated fatty acid-binding protein was rapidly digested by achromobacter lysyl endopeptidase, bovine alpha-chymotrypsin, and staphylococcal v8 protease, while protein recombined with oleic acid was strongly refractory to proteolysis. this observation indicates that a striking change in the conformation ... | 1994 | 7896729 |
| isolation and characterization of an n-methylcarbamate insecticide-degrading methylotrophic bacterium. | a gram-negative bacterium which hydrolyzed aryl n-methylcarbamate insecticides was isolated from an agricultural soil which quickly degraded these pesticides. this organism, designated strain er2, grew on carbofuran as a sole source of carbon and nitrogen with a doubling time of 3 h in a mineral salts medium. the aromatic nucleus of the molecule was not metabolized, and carbofuran 7-phenol accumulated as the end product of metabolism. the insecticides carbaryl, bendiocarb, and propoxur were simi ... | 1993 | 7504430 |
| cloning and nucleotide sequence analysis of the colh gene from clostridium histolyticum encoding a collagenase and a gelatinase. | the colh gene encoding a collagenase was cloned from clostridium histolyticum jcm 1403. nucleotide sequencing showed a major open reading frame encoding a 116-kda protein of 1,021 amino acid residues. the deduced amino acid sequence contains a putative signal sequence and a zinc metalloprotease consensus sequence, hexxh. a 116-kda collagenase and a 98-kda gelatinase were copurified from culture supernatants of c. histolyticum. while the former degraded both native and denatured collagen, the lat ... | 1994 | 7961400 |
| handwashing machines, handwashing compliance, and potential for cross-contamination. | although handwashing is considered an important factor in the prevention of nosocomial infections, the optimal technique has not been determined and compliance is often difficult to obtain. handwashing compliance is particularly important in intensive care areas of the hospital. in an effort to improve hw compliance, the surgical intensive care unit in our hospital purchased three handwashing machines. four months after installation of the handwashing machines, an outbreak of methicillin-resista ... | 1994 | 7985823 |
| alternatively spliced isoform of p-selectin is present in vivo as a soluble molecule. | to demonstrate the presence of a soluble isoform of p-selectin predicted from cdna sequencing (johnston, g.i., bliss, g.a., newman, p.j., and mcever, r.p. (1990) j. biol. chem. 265, 21381-21385), we immunoisolated and compared structurally p-selectin from fresh frozen human plasma with that from washed intact platelets. plasma p-selectin was reactive with rabbit antiserum to a synthesized peptide (residues 762-774 of mature p-selectin) but was significantly less reactive with antibody to a pepti ... | 1994 | 7522232 |
| [action of polycarbon compounds on the oxidation of methanol and other cl compounds by methylotrophic bacteria]. | cells of the facultative methylotrophic bacteria achromobacter parvulus 1t, pseudomonas sp am1, ps. methylica 2k, ps. methylica 20t, ps. oleovorans 52z, ps. fluorescens 45k exhibit the ability to oxidize methanol only on the medium with methanol. at the growth of various methylotrophic bacteria on the media containing both methanol and succinate their ability to methanol oxidation is lost, and oxidation of formaldehyde and formate occur at a low rate. glucose, citrate, acetate and glycerol produ ... | 1983 | 6411135 |
| [bacterial formate dehydrogenase. substrate specificity and kinetic mechanism of s-formyl glutathione oxidation]. | the substrate specificity of nad-dependent formate dehydrogenase from the methylotrophic bacterium achromobacter parvulus t1 was studied. the kinetic mechanism of s-formyl glutathione oxidation was determined. the initial velocity studies and inhibition analysis were carried out. it was shown that the kinetic mechanism for the enzyme with s-formyl glutathione as a substrate is similar to that with formate and is rapid-equilibrium random. using independent methods, it was found that formate dehyd ... | 1983 | 6615926 |
| survey on antibiotic resistance in gram-negative non-fermentative bacteria other than pseudomonas in italy. | a survey was conducted to investigate the incidence and antibiotic resistance of clinical isolates of gram-negative non-fermentative bacteria, isolated during a one-year period in italy. overall, these microorganisms account respectively for 11.47% and 20.5% of all gram-negative bacteria. the most representative species isolated during the study were: acinetobacter, flavobacterium, alcaligenes, achromobacter and xanthomonas species. as regards their antimicrobial pattern of resistance, this surv ... | 1994 | 8071672 |
| sweet potato beta-amylase. primary structure and identification of the active-site glutamyl residue. | the complete amino acid sequence of a subunit of sweet potato beta-amylase, a homotetramer, was established by sequence analysis of peptides obtained by digestions with achromobacter protease i and staphylococcus aureus v8 protease and by cyanogen bromide cleavage of the s-carboxymethylated subunit. the subunit of the enzyme is a single polypeptide consisting of 498 amino acid residues. it showed 50-60% identity in the amino acid sequence with those of beta-amylases from soybean and barley, whil ... | 1993 | 8103452 |
| purification and characterization of bothrombin, a fibrinogen-clotting serine protease from the venom of bothrops jararaca. | a fibrinogen-clotting enzyme (bothrombin) was purified from the venom of bothrops jararaca. bothrombin showed m(r) values of 33,000 under nonreducing and 35,000 under reducing conditions on sds polyacrylamide gel electrophoresis and specific fibrinogen-clotting activity equivalent to 814-904 nih alpha-thrombin units/mg. diisopropyl fluorophosphate totally abolished its activity, but hirudin, a specific alpha-thrombin inhibitor, had negligible effect on bothrombin activity. unlike alpha-thrombin, ... | 1994 | 8110787 |
| structure of alcaligenes faecalis nitrite reductase and a copper site mutant, m150e, that contains zinc. | the structures at 2.0 and 2.25 a resolution of native and recombinant nitrite reductase from alcaligenes faecalis show that they are identical to each other and very similar to nitrite reductase from achromobacter cycloclastes. the crystallographic structure of a mutant, m150e, which unlike the wild-type protein cannot be reduced by pseudoazurin, shows that the glutamate replacement for methionine binds to a metal at the type i cu site via only one oxygen. anomalous scattering data collected at ... | 1995 | 7547950 |
| ornithine-containing lipids in thiobacillus a2 and achromobacter sp. | 20 bacterial strains (corresponding to 16 species) were screened for ornithine lipids. only two species (thiobacillus a2 and achromobacter sp.) turned out to contain ornithine lipids (2.71 mmol/100 g and 0.38 mmol/100 g bacterial dry weight, respectively). in both ornithine lipids, a 3-hydroxy fatty acid was amide-linked to the alpha-amino group of ornithine, a normal fatty acid was ester-linked to the 3-hydroxy group of the former. the predominant fatty acids were 18:1(11) and 3-hydroxy-20:1(13 ... | 1984 | 6698001 |
| corneal ulcer due to achromobacter xylosoxidans. | we report a case of corneal ulcer caused by the opportunistic organism achromobacter xylosoxidans which developed during chronic topical steroid treatment of an eye with neovascular glaucoma. a. xylosoxidans has probably been underreported as a cause of ocular infection because of confusion between this organism and other gram-negative organisms, particularly pseudomonas. a. xylosoxidans is resistant to aminoglycosides and some cephalosporins but not carbenicillin. this difference in antibiotic ... | 1984 | 6733072 |
| l-lysine: 2-oxoglutarate 6-aminotransferase. subunit structure composed of non-identical polypeptides and pyridoxal 5'-phosphate-binding subunit. | l-lysine:2-oxoglutarate 6-aminotransferase from flavobacterium lutescence (= achromobacter liquidum) has been shown to be composed of one each of four non-identical subunits, a, b1, b2, and c. the subunits were isolated by gel filtration, and deae-cellulose chromatography in the presence of 8 m urea. their molecular weights were determined by ultracentrifugation, gel electrophoresis and gel filtration: subunit a 24,000; b1 28,000; b2 28,000; c 45,000. these subunits were all different in amino a ... | 1980 | 6771255 |
| resonance raman spectroscopy of the azurin his117gly mutant. interconversion of type 1 and type 2 copper sites through exogenous ligands. | the copper center of the pseudomonas aeruginosa his117gly azurin mutant is accessible to exogenous ligands through an aperture in its surface created by the removal of the endogenous imidazole ligand. depending on the exogenous ligand, a surprising variety of type 1 and type 2 copper sites can be obtained that are readily distinguished by electronic, epr, and resonance raman (rr) spectroscopy. the rr spectrum of type 1 h117g with exogenous imidazole is nearly identical to that of wild-type azuri ... | 1993 | 8241136 |
| achromobacter xylosoxidans osteomyelitis. | | 1993 | 8280199 |
| purification and characterization of clostridium perfringens 120-kilodalton collagenase and nucleotide sequence of the corresponding gene. | clostridium perfringens type c ncib 10662 produced various gelatinolytic enzymes with molecular masses ranging from approximately 120 to approximately 80 kda. a 120-kda gelatinolytic enzyme was present in the largest quantity in the culture supernatant, and this enzyme was purified to homogeneity on the basis of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the purified enzyme was identified as the major collagenase of the organism, and it cleaved typical collagenase substrates such ... | 1994 | 8282691 |
| identification of the electrophilic substrate-binding site of glutathione s-transferase p by photoaffinity labeling. | we determined the electrophilic substrate-binding site of rat glutathione s-transferase p (gst-p) by photoaffinity labeling using the photosensitive compound s-[2-(2-fluoro-4-nitrophenoxy)ethyl]glutathione. this photosensitive glutathione analogue inhibited the catalytic activity in a competitive manner against both glutathione and 1-chloro-2,4-dinitrobenzene, a putative electrophilic substrate. the enzyme kinetics indicated that the photoactivatable glutathione analogue was specifically bound a ... | 1995 | 7556138 |
| ochrobactrum anthropi bacteremia: report of four cases and short review. | ochrobactrum anthropi, formerly "achromobacter" cdc group vd, is a nonfermentative, nonfastidious gram-negative bacillus, that only recently has been given attention as a potential human pathogen. over a 2-year period, we observed four patients with multiple blood cultures that were positive for the organism. the patients had acute leukemia as underlying disease, and presented with clinical and microbiologic features consistent with catheter-related bacteremia. in three of the patients the infec ... | 1993 | 8300247 |
| studies on a new proteolytic enzyme from a chromobacter lyticus m497-1. i. purification and some enzymatic properties. | achromobacter lyticus m497-1 produces three kinds of alkaline proteases (protease i, ii and iii) in culture medium along with the bacteriolytic enzyme (masaki, t., nakamura, k., isono, m. and soejima, m. (1978) agric. biol. chem. 42, 1443--1445). among these three proteases, achromobacter protease i (ec 3.4.21.-) shows strict splitting for lysine residues at the carboxyl side of the splitting point. this enzyme was purified through a sequence of benzalkonium chloride treatment, acetone fractiona ... | 1981 | 6791693 |
| improvement in the persistence of microbial asparaginase and glutaminase in the circulation of the rat by chemical modifications. | three enzymes used in cancer chemotherapy (asparaginases from escherichia coli and erwinia carotovora and glutaminase from achromobacter) were each reacted with four amino specific reagents (ethyl acetimidate, o-methylisourea, succinic anhydride, and formaldehyde/sodium borohydride). the half-lives of the modified enzymes measured in the blood of rats showed that guanidation, acetimidation and reductive alkylation were more likely to increase the persistence of the native enzymes than succinylat ... | 1981 | 6794649 |
| [relationship between water pollution and bacterial flora in river water]. | to clarify the relationship between water pollution and bacterial flora in rivers, 132 samples of river water were collected at eleven stations of the chikuma-sai river system from april 1985 to march 1986, and 29 biological-physicochemical examinations for indices of water pollution were done using these samples. species and genus of bacteria in 485 isolates from bacterial flora were identified. although variations of bacterial flora were seen among sampling stations and sampling seasons, most ... | 1993 | 8377255 |
| osteomyelitis due to achromobacter xylosoxidans. | | 1993 | 8399894 |
| a plasmid-mediated cephalosporinase from achromobacter species. | an unusual cephalosporinase in achromobacter species was characterized biochemically; the enzyme had a pi of 8.1 and a molecular mass of 36,200 daltons, and it was not inhibited by p-chloromercuribenzoate or cloxacillin. specific antiserum neutralized enzymatic activity. agarose gel electrophoresis of the dna of two strains (mulb 906 and mulb 912) revealed at least three plasmid bands; cured strains demonstrated a simultaneous loss of beta-lactamase and plasmid dna. resistance to beta-lactam ant ... | 1982 | 6804578 |
| chemical modification of lysine residues in bacterial formate dehydrogenase. | specific modification of 4.4 lysine residues per molecule of formate dehydrogenase, from the methylotrophic bacterium achromobacter parvulus i by pyridoxal, results in complete inactivation of the enzyme. the concentration effect of the modifying agent and substrates on the inactivation of formate dehydrogenase has been studied. coenzymes do not protect the enzyme from inactivation. complete maintenance of enzyme activity was achieved in the presence of saturating concentrations of the formate a ... | 1982 | 6817795 |
| cellular fatty acid compositions of "achromobacter groups b and e". | strains of "achromobacter groups b and e" were examined for cellular fatty acid (cfa) composition to evaluate their chemical relatedness to known bacterial species and groups. the cfas were liberated from whole cells by base hydrolysis, methylated, and analyzed by capillary gas-liquid chromatography. the cfa profiles of the two groups were identical and were distinct from cfa profiles of all other bacteria we have previously tested. these data provide support for results from whole-cell protein ... | 1993 | 8463379 |
| cell-surface collagen-binding protein in the procaryote achromobacter iophagus. | collagen and its high-molecular-weight fragments specifically induce an extracellular collagenase (ec 3.4.24.8) in the gram-negative achromobacter iophagus. during the induction process the inducer is concentrated on the bacterial outer membrane. two-dimensional electrophoresis of 125i-labelled outer membrane proteins has shown that, in particular, the amount of one protein which is already present on the surface of non-induced bacteria increases quantitatively when the inducer is added. after 1 ... | 1983 | 6824647 |
| pro-major basic protein has three types of sugar chains at the pro-portion. | the amino-acid sequence of purified recombinant pro-major basic protein from chinese hamster kidney cells was determined to verify the primary structure and glycosylation sites. reduced and s-carboxamidemethylated protein was first digested with achromobacter proteinase i. each peptide was characterized by amino-acid analysis and amino-acid sequence analysis. we could identify all the peptides which were expected from the pro-major basic protein cdna sequence. sequence analysis and deglycosylati ... | 1993 | 8507662 |
| phosphatidylcholine-specific phospholipase c from achromobacter xylosoxidans. | a new phosphatidylcholine-specific phospholipase c (ec 3.1.4.3.) has been isolated from the culture broth of achromobacter xylosoxidans. growth conditions were optimized for maximum production of the enzyme. a temperature-sensitive synthesis was established. chromatography on cm sephadex and polyacrylamide gel electrophoresis of the native enzyme revealed a number of isozymes. the water soluble substrate for phospholipase p-nitrophenylphosphorylcholine was not hydrolysed by the enzyme. | 1993 | 8511996 |
| in vitro activity of new cephalosporins against multi-resistant gram-negative bacteria. | the in vitro sensitivities of 47 multi-resistant gram-negative bacilli against six third generation cephalosporins were investigated. overall, moxalactam was the most active compound and cefoperazone the least active. ceftizoxime and ceftazidime were very active against enterobacteriaceae. ceftriaxone and ceftizoxime were active against escherichia coli and klebsiella spp., but were inactive against pseudomonas spp.; achromobacter sp. was resistant to all drugs, while citrobacter freundii was se ... | 1983 | 6832840 |
| structure of azurin from achromobacter xylosoxidans ncib11015 at 2.5 a resolution. | the crystal structure of azurin from a denitrifying bacterium, achromobacter xylosoxidans ncib11015, has been refined at 2.5 a resolution using diffraction data obtained by means of synchrotron radiation at kek. crystals suitable for x-ray experiment were obtained by the macro-seeding method and an intensity data were obtained on imaging plates mounted on a weissenberg camera (rmerge = 0.09). the initial model was obtained by the molecular replacement method using the structure of azurin from al ... | 1994 | 7706206 |
| a removable spacer peptide in an alpha-factor-leader/insulin precursor fusion protein improves processing and concomitant yield of the insulin precursor in saccharomyces cerevisiae. | an alpha-factor leader/insulin precursor fusion protein was produced in saccharomyces cerevisiae and metabolically labeled in order to analyse the efficiency of maturation and secretion. a substantial fraction of the secreted material was found in a hyperglycosylated unprocessed form, indicating incomplete kex2p endopeptidase maturation. introduction of a spacer peptide (eaeaeak) after the dibasic kex2p site, creating a n-terminal extension of the insulin precursor, greatly increased the kex2p c ... | 1996 | 8621069 |
| mouse gamma-casein cdna: pcr cloning and sequence analysis. | a partial amino acid sequence of mouse gamma-casein was determined on a gas-phase amino acid sequencer. the n-terminal sequence (23 residues) was obtained using native gamma-casein, and the c-terminal sequence (13 residues) was determined with a corresponding peptide. the c-terminal peptide was isolated by affinity chromatography on an anhydrotrypsin agarose column after digestion of gamma-casein with achromobacter lysyl-endopeptidase. a cdna (507 base pairs) encoding the mature protein of gamma ... | 1993 | 7763793 |
| meningitis caused by alcaligenes (achromobacter) xylosoxidans associated with epidural catheter. | | 1995 | 8655217 |
| hydrolysis of s-2-aminoethylcysteinyl peptide bond by achromobacter protease i. | the substrate specificity of achromobacter protease i (api) was examined for s-2-aminoethyl(ae)cysteinyl bonds in bz-aec-ome/oet, bz-aec-nh2, and ae-insulin b chain. the protease hydrolyzed all of the tested ae-cysteinyl bonds at the same rate as that of lysyl bonds. kinetic parameters were estimated for this hydrolysis reaction. | 1994 | 7764517 |
| site-specific glycosylation of bovine butyrophilin. | our previous studies showed that the n-linked sugar chains of most bovine glycoproteins from milk fat globule membranes (mfgm) contain the galnac beta 1-->4glcnac group [sato et al. (1993) j. biochem. 114, 890-900]. since expression of the disaccharide structure is influenced by peptide sequences near the glycosylation sites [smith and baenziger (1992) proc. natl. acad. sci. usa 89, 329-333], the site-specificity of the n-acetylgalactosaminylated sugar chains was investigated using bovine butyro ... | 1995 | 7775382 |
| delta 3, delta 2-enoyl-coa isomerase is the protein that copurifies with human glutathione s-transferases from s-hexylglutathione affinity matrices. | an unidentified 30 kda protein frequently copurifies with human glutathione s-transferases from s-hexyl-glutathione affinity matrices. application of two-step sequential affinity chromatographic methods yielded a homogeneous preparation of that protein from human liver specimens. the protein was digested with achromobacter protease i, and sequences of peptides resolved by h.p.l.c. showed a high degree of identity with those of rat mitochondrial delta 3, delta 2-enoyl-coa isomerase. the human pro ... | 1994 | 7818490 |
| purification and primary structure of a myotoxic lysine-49 phospholipase a2 with low lipolytic activity from trimeresurus gramineus venom. | four acidic phospholipase a2 (pla2) isozymes named pla2-i, ii, iii and iv have previously been isolated from trimeresurus gramineus (green habu snake) venom and sequenced [oda et al. (1991) toxicon 29, 157; fukagawa et al. (1992) toxicon 30, 1131; fukagawa et al. (1993) toxicon 31, 957]. they contain aspartate-49 which is known to bind ca2+, essential for catalysis. in the present study, a basic pla2 named pla2-v containing lysine-49 was newly isolated from the same snake venom. its isoelectric ... | 1995 | 8744986 |
| [bacteremia caused by achromobacter xylosidans]. | | 1996 | 8750544 |
| lysyl endopeptidase of achromobacter lyticus. | | 1994 | 7845202 |
| incidence of pyrexia in patients undergoing haemodialysis. | four patients on maintenance haemodialysis at the lagos university teaching developed pyrogenic reactions during treatment. blood cultures were negative but the haemodialysates were grossly contaminated, mostly with gram-negative bacilli, implicating their endotoxin as the cause of the observed pyrogenicity. the water reservoirs supplying the dialysis centre were grossly contaminated (counts 2.0 x 10(2) cfuml-1) with gram-negative bacteria such as pseudomonas aeruginosa, ps. fluorescens, alcalig ... | 1996 | 8855673 |
| metabolic and structural changes in pseudomonas aeruginosa, achromobacter cdc and agrobacterium radiobacter cells injured in parenteral fluids. | the long term metabolic changes of three oxidase positive microorganisms (pseudomonas aeruginosa, agrobacterium radiobacter and achromobacter cdc) all isolated from aquatic environment, were defined after they were inoculated in three parenteral fluids: lactated ringer's solution, sodium chloride 0.9% and dextrose 5%. the number of microorganisms introduced into the parenteral products was adjusted to 10(5) bacteria/ml and left at room temperature (20-22 degrees c) for 30 days. their enzymatic a ... | 1994 | 7850451 |
| replacement of methionine as the axial ligand of achromobacter cycloclastes cytochrome c554 at high ph values revealed by absorption, epr and mcd spectroscopy. | cytochrome c554 from the denitrifying bacterium achromobacter cycloclastes is a monoheme class ii c-type cytochrome with a his-met axial coordination at neutral ph. the amino acid composition and the n-terminal sequence of the cytochrome have been determined. subsequent determination of the ph-dependence of the redox potential and examination of the epr and mcd spectra of ferricytochrome c554 revealed a new form at high ph values made apparent with both spectroscopies. these observations are con ... | 1994 | 7945350 |
| enzymatic regioselective acylation of 3-o-beta-d-galactopyranosyl-sn-glycerol by achromobacter sp. lipase. | | 1994 | 8004727 |
| identification of three catalytic triad constituents and asp-225 essential for function of lysine-specific serine protease, achromobacter protease i. | achromobacter protease i is a lysine-specific serine protease that achromobacter lyticus m497-1 extracellularly secretes. the structural aspects necessary for the protease to function were investigated by means of site-directed mutagenesis to identify the constituents of the catalytic triad and the amino acid residue responsible for lysine specificity. the precursor molecules, which were produced by substitution of his-57, asp-113, or ser-194 for alanine, could not be converted to the mature for ... | 1994 | 8006007 |
| cloning and expression of the gene for hydroxypyruvate reductase (d-glycerate dehydrogenase from an obligate methylotroph hyphomicrobium methylovorum gm2. | the gene encoding hydroxypyruvate reductase, catalyzing the asymmetric reduction of hydroxypyruvate to d-glycerate, and its flanking regions were isolated from a methylotrophic bacterium, hyphomicrobium methylovorum gm2. nucleotide sequencing of the recombinant plasmids revealed that the hydroxypyruvate-reductase gene codes for the 322-amino-acid protein with calculated molecular mass 35,726 da. the sequence was confirmed by sequencing the intact enzyme and peptides obtained by digestion of the ... | 1994 | 8055948 |
| [bacteremia caused by alcaligenes (achromobacter) xylosoxidans. description of 3 cases and review of the literature]. | alcaligenes (achromobacter) xylosoxidans occasionally cause infections, mainly in immunocompromised hosts. | 1996 | 8991439 |
| [collagenolytic enzymes synthesized by microorganisms]. | the review uses data obtained by the authors and available in the literature to discuss microbial collagenolytic enzymes, widely employed in scientific research, biotechnology, and medicine. collagenases differing in their structure and the specificity of their action on collagen fibrils were isolated from bacteria (including marine isolates), actinomycetes, and fungi. collagenases produced by clostridium histolyticum and achromobacter iophagus are the best studied enzymes; both are metalloenzym ... | 1996 | 8992239 |
| location of cysteine and cystine residues in s-ribonucleases associated with gametophytic self-incompatibility. | s-ribonucleases (s-rnases) that cosegregate with s-alleles in the styles of solanaceous and rosaceous plants are associated with gametophytic self-incompatibility (gsi). the amino acid sequences of many s-rnases have been derived from cdna sequences, but the state of half-cystines has not been clarified. we report the locations of the two free cysteine residues and four disulfide bridges of tobacco s6-rnase and of the four disulfide bridge of japanese pear s4-rnase. the protein was first s-pyrid ... | 1996 | 9022690 |
| characterization and distribution of o-glycosylated carbohydrates in the cell adhesion molecule, contact site a, from dictyostelium discoideum. | this paper presents further investigation of the properties of carbohydrate ii in the cell adhesion molecule, contact site a, from dictyostelium discoideum. a purified contact site a was digested with achromobacter protease i to produce a 31-kda fragment to which carbohydrate ii was mainly bound and a 21-kda fragment containing the nh2 terminus of contact site a, which was identified as ala-pro-thr-ile-thr-ala. the nh2 terminus of the 31-kda fragment was thr-glu-ala-thr-thr-ser. it was estimated ... | 1997 | 9024799 |
| nitrite reductase from achromobacter xylosoxidans forms stable trimers in dilute solution. | | 1997 | 9056938 |
| recurrent septicaemia due to "achromobacter group b". | | 1997 | 9138138 |
| d-lysine production from l-lysine by successive chemical racemization and microbial asymmetric degradation. | in order to develop a practical process for d-lysine production from l-lysine, successive chemical racemization and microbial asymmetric degradation were investigated. the racemization of l-lysine proceeded quantitatively at elevated temperatures. a sample of 1000 strains of bacteria, fungi, yeast and actinomyces were screened for the ability to degrade l-lysine asymmetrically. microorganisms belonging to the achromobacter, agrobacterium, candida, comamonas, flavobacterium, proteus, providencia, ... | 1997 | 9163947 |
| crucial role of intralobe peptide-peptide interactions in the uptake and release of iron by ovotransferrin. | the mechanism for reversible iron binding in the n-terminal lobe of ovotransferrin was investigated by a protein fragmentation approach. the iron-saturated n-terminal half-molecule of ovotransferrin was proteolyzed into large 30-kda (1-279) and small 6-kda (280-332) fragments by a single cleavage with achromobacter protease i, producing a stable nicked form. for the separation of the two fragments, denaturing conditions were required. the isolated large fragment contained all four iron-coordinat ... | 1994 | 8120023 |
| intravenous line infection due to ochrobactrum anthropi (cdc group vd) in a normal host. | ochrobactrum anthropi, formerly known as achromobacter species (cdc group vd), is an aerobic, gram-negative bacillus widely distributed in aquatic environments. most important, it has been implicated as a cause of intravenous line infection in immunocompromised hosts with solid tumors or hematologic malignancies. trimethoprim-sulfamethoxazole and aminoglycosides are usually active against o. anthropi, but this organism is usually resistant to beta-lactam antibiotics. because o. anthropi is a low ... | 1997 | 9257145 |
| crystallization and preliminary x-ray studies on pseudoazurin from achromobacter cycloclastes iam1013. | new crystals of a blue copper protein, pseudoazurin from denitrifier achromobacter cycloclastes iam1013, have been obtained by means of vapor diffusion with ammonium sulfate as a precipitant at ph 6.0 and 4 degrees c. the crystals belong to the orthorhombic system, space group p2(1)2(1)2(1), with unit cell dimensions of a = 56.69(2), b = 61.53(2), and c = 30.20(1) a. the asymmetric unit includes one molecule of pseudoazurin with a vm value of 2.04 a3/da. the crystals are so stable against x-ray ... | 1993 | 8138527 |