| putrescine, cadaverine, and indole production by bacteria isolated from wild and aquacultured penaeid shrimp stored at 0, 12, 24, and 36 degrees c. | putrescine, cadaverine, and indole production capabilities of bacteria isolated from wild domestic and aquacultured ni-caraguan penaeid shrimp in progressive decomposition states were evaluated. the numbers and types of microorganisms responsible for the production of putrescine, cadaverine, and indole in wild and aquacultured shrimp increased with increasing decomposition temperature and time. throughout the storage experiments, mean aerobic plate counts (log/g) ranged from 4.5 to 9.7 and 4.5 t ... | 2004 | 14717362 |
| inhibition of biological reductive dissolution of hematite by ferrous iron. | bacterial dissimilatory iron reduction is self-inhibited by the production of ferrous [fe(ii)] iron resulting in diminished iron reduction as fe(ii) accumulates. experiments were conducted to investigate the mechanisms of fe(ii) inhibition employing the dissimilatory metal-reducing bacterium shewanella putrefaciens strain cn32 under nongrowth conditions in a system designed to minimize precipitation of ferrous iron minerals. after an initial period (ca. 1 day) of relatively rapid iron reduction, ... | 2004 | 14740735 |
| toxic effects of chromium(vi) on anaerobic and aerobic growth of shewanella oneidensis mr-1. | cr(vi) was added to early- and mid-log-phase shewanella oneidensis (s. oneidensis) mr-1 cultures to study the physiological state-dependent toxicity of cr(vi). cr(vi) reduction and culture growth were measured during and after cr(vi) reduction. inhibition of growth was observed when cr(vi) was added to cultures of mr-1 growing aerobically or anaerobically with fumarate as the terminal electron acceptor. under anaerobic conditions, there was immediate cessation of growth upon addition of cr(vi) i ... | 2004 | 14763828 |
| role of microbes in the smectite-to-illite reaction. | temperature, pressure, and time have been thought to control the smectite-to-illite (s-i) reaction, an important diagenetic process used for petroleum exploration. we demonstrated that microorganisms can promote the s-i reaction by dissolving smectite through reduction of structural fe(iii) at room temperature and 1 atmosphere within 14 days. this reaction typically requires conditions of 300 degrees to 350 degrees c, 100 megapascals, and 4 to 5 months in the absence of microbial activity. these ... | 2004 | 14764877 |
| phenazines and other redox-active antibiotics promote microbial mineral reduction. | natural products with important therapeutic properties are known to be produced by a variety of soil bacteria, yet the ecological function of these compounds is not well understood. here we show that phenazines and other redox-active antibiotics can promote microbial mineral reduction. pseudomonas chlororaphis pcl1391, a root isolate that produces phenazine-1-carboxamide (pcn), is able to reductively dissolve poorly crystalline iron and manganese oxides, whereas a strain carrying a mutation in o ... | 2004 | 14766572 |
| a new isolation method for labyrinthulids using a bacterium, psychrobacter phenylpyruvicus. | a new isolation method for labyrinthulids, marine microbes with spindle-shaped vegetative cells and gliding movement, is presented. the method for isolating labyrinthulids has been found to be more difficult and less reproducible than that for thraustochytrids, classified in the same order. so far serum seawater agar fortified with antibiotics has been proposed to be the best for isolation of labyrinthulids. the method presented here involves placing plant samples on an agar medium on which a ma ... | 2001 | 14961392 |
| specification of amino acid residues essential for the catalytic reaction of cold-active protein-tyrosine phosphatase of a psychrophile, shewanella sp. | protein-tyrosine phosphatase [ec 3.1.3.48] from a psychrophile, shewanella sp. shows high activity at low temperatures and has the conserved amino acid sequence of protein-ser/thr-phosphatases. site-directed mutagenesis with the conserved amino acid residues indicated that his148 could be important as a general acid catalyst and asp115 assists the protonation with his148 of the leaving group of a substrate, and that asp76 and asp112 were involved in binding to magnesium ions. | 2004 | 14981312 |
| isolation and characterization of integron-containing bacteria without antibiotic selection. | the emergence of antibiotic resistance among pathogenic and commensal bacteria has become a serious problem worldwide. the use and overuse of antibiotics in a number of settings are contributing to the development of antibiotic-resistant microorganisms. the class 1 and 2 integrase genes (inti1 and inti2, respectively) were identified in mixed bacterial cultures enriched from bovine feces by growth in buffered peptone water (bpw) followed by integrase-specific pcr. integrase-positive bacterial co ... | 2004 | 14982773 |
| vanadium(v) reduction by shewanella oneidensis mr-1 requires menaquinone and cytochromes from the cytoplasmic and outer membranes. | the metal-reducing bacterium shewanella oneidensis mr-1 displays remarkable anaerobic respiratory plasticity, which is reflected in the extensive number of electron transport components encoded in its genome. in these studies, several cell components required for the reduction of vanadium(v) were determined. v(v) reduction is mediated by an electron transport chain which includes cytoplasmic membrane components (menaquinone and the tetraheme cytochrome cyma) and the outer membrane (om) cytochrom ... | 2004 | 15006760 |
| direct observation of microbial inhibition of calcite dissolution. | vertical scanning interferometry (vsi) provides a method for quantification of surface topography at the angstrom to nanometer level. time-dependent vsi measurements can be used to study the surface-normal retreat across crystal and other solid surfaces during dissolution or corrosion processes. therefore, vsi can be used to directly and nondestructively measure mineral dissolution rates with high precision. we have used this method to compare the abiotic dissolution behavior of a representative ... | 2004 | 15006787 |
| dissociation behavior of doubly-charged tryptic peptides: correlation of gas-phase cleavage abundance with ramachandran plots. | analysis of fragmentation patterns from 5654 unique doubly charged tryptic peptides is obtained. great variability of average relative abundance of bond cleavage is found between different amino acid combinations. there exist similarities as well as differences between b and y ions. strong enhancement or suppression of cleavage gives insight into possible chemical interactions at reactive conformations formed by preferred phi-psi angles. | 2004 | 15012117 |
| the structure of the core region of the lipopolysaccharide from shewanella algae bry, containing 8-amino-3,8-dideoxy-d-manno-oct-2-ulosonic acid. | the structure of the carbohydrate backbone of the lipid a-core region of the lps from shewanella algae strain bry was analysed. the lps was n,o-deacylated to give three products, which were isolated and studied by chemical methods, nmr and mass spectrometry: [carbohydrate structures: see text]. all monosaccharides except l-rhamnose had the d-configuration. this lps presents a second example (after s. oneidensis) of the structure with a novel linking unit between the core and lipid a moieties, 8- ... | 2004 | 15013415 |
| shewanella gaetbuli sp. nov., a slight halophile isolated from a tidal flat in korea. | a gram-negative, motile, non-spore-forming, rod-shaped strain, tf-27(t) (=kccm 41648(t)=jcm 11814(t)), was isolated from a tidal flat in korea. this organism grew well at 25-35 degrees c, with optimum growth at 30 degrees c. strain tf-27(t) grew optimally in the presence of 2 % nacl; it did not grow without nacl or in the presence of >8 % nacl. strain tf-27(t) simultaneously contained both menaquinones and ubiquinones as isoprenoid quinones. the predominant menaquinone was mk-7 and the predomina ... | 2004 | 15023965 |
| identification of a novel steroid inducible gene associated with the beta hsd locus of comamonas testosteroni. | comamonas testosteroni is a soil bacterium, which can use a variety of steroids as carbon and energy source. even if it can be estimated that the complete degradation of the steroid nucleus requires more than 20 enzymatic reactions, the complete molecular characterization of the genes encoding these steroid degradative enzymes as well as the genetic organization of them remain to be elucidated. we have previously reported the cloning and nucleotide sequence of two steroid-inducible genes, beta h ... | 2004 | 15026087 |
| possible involvement of an fkbp family member protein from a psychrotrophic bacterium shewanella sp. sib1 in cold-adaptation. | a psychrotrophic bacterium shewanella sp. strain sib1 was grown at 4 and 20 degrees c, and total soluble proteins extracted from the cells were analyzed by two-dimensional polyacrylamide gel electrophoresis. comparison of these patterns showed that the cellular content of a protein with a molecular mass of 28 kda and an isoelectric point of four greatly increased at 4 degrees c compared to that at 20 degrees c. determination of the n-terminal amino acid sequence, followed by the cloning and sequ ... | 2004 | 15030488 |
| shewanella putrefaciens isolated in a case of ventilator-associated pneumonia. | we report the isolation of the aquatic gram-negative bacterium shewanella putrefaciens from the bronchoalveolar lavage in a case of ventilator-associated pneumonia. to our knowledge, this species has never been isolated from the lower respiratory tract before as a potential pathogen. | 2004 | 15031580 |
| [investigation of the bacteria in the seawater of xisha in the south china sea and their antibiotic sensitivity profile]. | to investigate the distribution and antibiotic sensitivity of the bacteria in the seawater of xisha in the south china sea. | 2004 | 15041561 |
| from hyperthermophiles to psychrophiles: the structural basis of temperature stability of the amino acid dehydrogenases. | the determination and comparison of the structures of a number of glutamate dehydrogenases that span the spectrum of thermal stability from mesophiles to hyperthermophiles have revealed that the formation of extended ion-pair networks is a major stabilizing feature in the adaptation of the organism to life at 100 degrees c. analysis of the structures of other hyperthermophilic proteins has shown that ion-pair networks also play roles in their stabilization, including the phosphoglucose isomerase ... | 2004 | 15046585 |
| characterisation of a tetrasaccharide released on mild acid hydrolysis of lps from two rough strains of shewanella species representing different dna homology groups. | a reducing tetrasaccharide of the following structure was released by mild acid hydrolysis of r-type lps from shewanella putrefaciens strains ncimb 10472 and 10473. the same tetrasaccharide containing acetal-linked open-chain galnac is present in the core region of lps from s. oneidensis strain mr-1 and may be characteristic of genomic groups ii and iii of s. putrefaciens and related strains. (1s)-d-galanac-(1-->4,6)-alpha-d-galp-(1-->6)-alpha-d-galp-(1-->3)-d-gal. | 2004 | 15063209 |
| isolation of extremophiles with the detection and retrieval of shewanella strains in deep-sea sediments from the west pacific. | tests to detect the presence of piezophilic shewanella strains in the deep-sea sediments of the west, mid- and east pacific at different depths were done by amplification of previously identified pressure-regulated operons (orf1,2 and orf3). the operon fragments were detected in all the deep-sea sediment samples, indicating the broad presence of piezophilic deep-sea shewanella species or related species in the deep-sea sediments across the pacific. extremophiles were isolated from the deep-sea s ... | 2004 | 15064982 |
| biogeochemical influence on transport of chromium in manganese sediments: experimental and modeling approaches. | hexavalent chromium (cr(vi)) was reduced to immobile and nontoxic cr(iii) by a dissimilatory metal reducing bacteria, shewanella alga simidu (bry-mt) atcc 55627. a series of kinetic batch and dynamic column experiments were conducted to provide an understanding of cr(vi) reduction by the facultative anaerobe bry-mt. reduction of cr(vi) was rapid (within 1 h) in columns packed with quartz sand and bacteria, whereas cr(vi) reduction by bry-mt was delayed (57 h) in the presence of beta-mno2-coated ... | 2004 | 15068867 |
| biosynthesis and dietary uptake of polyunsaturated fatty acids by piezophilic bacteria. | the biochemistry of piezophilic bacteria is unique in that piezophiles produce polyunsaturated fatty acids (pufas). a pertinent question is if piezophilic bacteria synthesize pufa de novo, through dietary uptake, or both. this study was undertaken to examine the biosynthesis and cellular uptake of pufas by piezophilic bacteria. a moderately piezophilic (shewanella violacea dss12) and two hyperpiezophilic bacteria (s. benthica db21mt-2 and moritella yayanosii db21mt-5) were grown under 50 mpa (me ... | 2004 | 15081997 |
| genetic and biochemical characterization of a chromosome-encoded carbapenem-hydrolyzing ambler class d beta-lactamase from shewanella algae. | a chromosome-encoded beta-lactamase gene from shewanella algae clinical isolate kb-1 was cloned and expressed in escherichia coli. it encoded the ambler class d enzyme oxa-55, sharing less than 55% identity with any other oxacillinases. although conferring a narrow-spectrum beta-lactam resistance phenotype, oxa-55 had carbapenem-hydrolyzing activity that mirrored the reduced susceptibility to imipenem observed in s. algae kb-1. very similar oxacillinases were found in other s. algae isolates. | 2004 | 15105119 |
| identification of 42 possible cytochrome c genes in the shewanella oneidensis genome and characterization of six soluble cytochromes. | through pattern matching of the cytochrome c heme-binding site (cxxch) against the genome sequence of shewanella oneidensis mr-1, we identified 42 possible cytochrome c genes (27 of which should be soluble) out of a total of 4758. however, we found only six soluble cytochromes c in extracts of s. oneidensis grown under several different conditions: (1) a small tetraheme cytochrome c, (2) a tetraheme flavocytochrome c-fumarate reductase, (3) a diheme cytochrome c4, (4) a monoheme cytochrome c5, ( ... | 2004 | 15107237 |
| probing domain mobility in a flavocytochrome. | the crystal structures of various different members of the family of fumarate reductases and succinate dehydrogenases have allowed the identification of a mobile clamp (or capping) domain [e.g., taylor, p., pealing, s. l., reid, g. a., chapman, s. k., and walkinshaw, m. d. (1999) nat. struct. biol. 6, 1108-1112], which has been proposed to be involved in regulating accessibility of the active site to substrate. to investigate this, we have constructed the a251c:s430c double mutant form of the so ... | 2004 | 15109257 |
| new method for rapid and sensitive quantification of sulphide-producing bacteria in fish from arctic and temperate waters. | the offensive, fishy, rotten h2s-off-odours in spoiled, aerobically and cold stored fish from arctic and temperate waters are generally caused by sulphide-producing bacteria (spb), mainly shewanella putrefaciens. in the present work, a new, rapid, simple and accurate method for estimation of the spb content in fish from these areas is described. the quantification is based on the formation rate of iron sulphide during growth of spbs incubated at 30 degrees c in a liquid growth medium containing ... | 2004 | 15163589 |
| structure of an acidic o-specific polysaccharide from marine bacterium shewanella fidelis kmm 3582t containing nepsilon-[(s)-1-carboxyethyl]-nalpha-(d-galacturonoyl)-l-lysine. | the o-specific polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of the marine bacterium shewanella fidelis type strain kmm 3582t and studied by sugar analysis along with 1h and 13c nmr spectroscopy including one-dimensional noe in difference mode and two-dimensional experiments. the polysaccharide was found to consist of linear tetrasaccharide repeating units containing nepsilon-[(s)-1-carboxyethyl]-nalpha-(d-galacturonoyl)-l-lysine and having the following structur ... | 2004 | 15183741 |
| the outer membrane protein omp35 affects the reduction of fe(iii), nitrate, and fumarate by shewanella oneidensis mr-1. | shewanella oneidensis mr-1 uses several electron acceptors to support anaerobic respiration including insoluble species such as iron(iii) and manganese(iv) oxides, and soluble species such as nitrate, fumarate, dimethylsulfoxide and many others. mr-1 has complex branched electron transport chains that include components in the cytoplasmic membrane, periplasm, and outer membrane (om). previous studies have implicated a role for anaerobically upregulated om electron transport components in the use ... | 2004 | 15212692 |
| [bacteremia in an obese patient with cellulitis and chronic ulceration in the lower extremity]. | | 2004 | 15228905 |
| genes regulated by torr, the trimethylamine oxide response regulator of shewanella oneidensis. | the torecad operon encoding the trimethylamine oxide (tmao) respiratory system of shewanella oneidensis is positively controlled by the tors/torr two-component system when tmao is available. activation of the tor operon occurs upon binding of the phosphorylated response regulator torr to a single operator site containing the direct repeat nucleotide sequence ttcatan4ttcata. here we show that the replacement of any nucleotide of one ttcata hexamer prevented torr binding in vitro, meaning that tor ... | 2004 | 15231782 |
| differential pressure resistance in the activity of rna polymerase isolated from shewanella violacea and escherichia coli. | rna polymerase was purified from the piezophile shewanella violacea dss12, and the transcriptional activity after pressure treatment was compared with that of the mesophile escherichia coli. application of pressure at 100 mpa for 30 min reduced the e. coli rna polymerase activity to 60% of the activity at atmospheric pressure, whereas the s. violacea rna polymerase maintained full activity, indicating that the s. violacea rna polymerase is more stable than its e. coli counterpart. this result wa ... | 2004 | 15241657 |
| correlated atomic force microscopy and fluorescence lifetime imaging of live bacterial cells. | we report on imaging living bacterial cells by using a correlated tapping-mode atomic force microscopy (afm) and confocal fluorescence lifetime imaging microscopy (flim). for optimal imaging of gram-negative shewanella oneidensis mr-1 cells, we explored different methods of bacterial sample preparation, such as spreading the cells on poly-l-lysine coated surfaces or agarose gel coated surfaces. we have found that the agarose gel containing 99% ammonium acetate buffer can provide sufficient local ... | 2004 | 15261059 |
| shewanella pacifica sp. nov., a polyunsaturated fatty acid-producing bacterium isolated from sea water. | six marine bacterial strains, kmm 3597t, kmm 3775, kmm 3590, kmm 3772, kmm 3605 and kmm 3601, that produce polyunsaturated fatty acids were isolated from sea water samples collected from different locations and depths in chazhma bay (sea of japan, pacific ocean) and characterized to clarify their taxonomic position. the dna g+c contents of these strains were 39.5-40.3 mol%. the level of dna hybridization between these strains was conspecific (83-96%), indicating that they represent a single geno ... | 2004 | 15280273 |
| shewanella affinis sp. nov., isolated from marine invertebrates. | four marine bacterial strains, designated kmm 3587t, kmm 3586, kmm 3821 and kmm 3822, were isolated from the sipuncula phascolosoma japonicum, a common inhabitant of troitza bay in the gulf of peter the great (sea of japan region), and from an unidentified hydrocoral species collected in makarov bay (iturup islands), kuril islands, north-west pacific ocean. the strains were characterized to clarify their taxonomic position. 16s rrna gene sequences of kmm 3587t and kmm 3586 indicated 99% similari ... | 2004 | 15280274 |
| engineering the interaction between micro-organisms and construction materials. | the influence of micro-organisms on degradation of mineral materials, cement bound systems, wood and steel is a rather new subject of research slowly becoming recognised by the 'classical' technical disciplines. an increasing amount of literature appears on biodeterioration of construction materials and microbial activity can not be neglected as a determining factor in the deterioration process. microbial communities interact in many different ways with mineral materials and their external envir ... | 2003 | 15296149 |
| intracellular manganese granules formed by a subsurface bacterium. | the demonstrated ability of prokaryotes to form internal metal oxide particles during active metabolism has been restricted to fe. mineral-bound mn(iv) is a known electron acceptor during dissimilatory metal reduction by shewanella putrefaciens, yet no internal deposits of mn have been reported to form during anaerobic respiration. we observed distinct nanometre-sized mn-rich granules in the cytoplasm when either birnessite or pyrolusite (beta-mno(2)) served as the electron acceptor during growt ... | 2004 | 15344929 |
| shewanella oneidensis mr-1 restores menaquinone synthesis to a menaquinone-negative mutant. | the mechanisms underlying the use of insoluble electron acceptors by metal-reducing bacteria, such as shewanella oneidensis mr-1, are currently under intensive study. current models for shuttling electrons across the outer membrane (om) of mr-1 include roles for om cytochromes and the possible excretion of a redox shuttle. while mr-1 is able to release a substance that restores the ability of a menaquinone (mk)-negative mutant, cma-1, to reduce the humic acid analog anthraquinone-2,6-disulfonate ... | 2004 | 15345428 |
| cloning and overproduction of the rpoz gene encoding an rna polymerase omega subunit from a deep-sea piezophilic shewanella violacea strain dss12. | we have cloned the rpoz gene, encoding rna polymerase omega protein, by pcr approach from the deep-sea piezophilic and psychrophilic bacterium, shewanella violacea strain dss12. the cloned gene, 285bp in length, was found to encode a protein consisting of 94 amino acid residues with a molecular mass of 10,327 da. significant homology was evident comparing the rpoz protein of s. violacea with that of shewanella oneidensis (69% identity), vibrio cholerae (65% identity), escherichia coli k-12 (64% ... | 2004 | 15346766 |
| octaheme tetrathionate reductase is a respiratory enzyme with novel heme ligation. | we have isolated a soluble cytochrome from shewanella oneidensis that contains eight covalently attached heme groups and determined its crystal structure. one of these hemes exhibits novel ligation of the iron atom by the epsilon-amino group of a lysine residue, despite its attachment via a typical cxxch motif. this heme is most likely the active site for tetrathionate reduction, a reaction catalyzed efficiently by this enzyme. | 2004 | 15361860 |
| studies on the interaction of nadph with rhodobacter sphaeroides biotin sulfoxide reductase. | rhodobacter sphaeroides biotin sulfoxide reductase (bsor) contains the bis(molybdopterin guanine dinucleotide)molybdenum cofactor and catalyzes the reduction of d-biotin-d-sulfoxide to biotin. this protein is the only member of the dimethyl sulfoxide reductase family of molybdopterin enzymes that utilizes nadph as the direct electron donor to the catalytic mo center. kinetic studies using stopped-flow spectrophotometry indicate that bsor reduction by nadph (>1000 s(-1)) is faster than steady-sta ... | 2004 | 15366932 |
| a rare cause of wound infection: shewanella putrefaciens. | shewanella putrefaciens has a wide geographical distribution, including all forms of sea water, fresh water, fish and soil. in humans, it is an unusual pathogen of wound infections. in this report, a wound infection was presented in a previously healthy man. | 2004 | 15370659 |
| cerebellar abscess caused by shewanella putrefaciens. | we report a case of cerebellar abscess secondary to chronic otitis by caused by shewanella putrefaciens and localized in the cerebellar hemisphere, in a paediatric patient. | 2004 | 15370679 |
| phylogenetic relationships among marine alteromonas-like proteobacteria: emended description of the family alteromonadaceae and proposal of pseudoalteromonadaceae fam. nov., colwelliaceae fam. nov., shewanellaceae fam. nov., moritellaceae fam. nov., ferrimonadaceae fam. nov., idiomarinaceae fam. nov. and psychromonadaceae fam. nov. | the phylogenetic relationships among marine alteromonas-like bacteria of the genera alteromonas, pseudoalteromonas, glaciecola, thalassomonas, colwellia, idiomarina, oceanimonas, oceanisphaera, shewanella, moritella, ferrimonas, psychromonas and several other genera of the 'gammaproteobacteria' were studied. results of 16s rrna gene sequence analyses revealed that some members of these genera formed several coherent groups at the family level. characteristic signature oligonucleotides for studie ... | 2004 | 15388743 |
| accumulation of mn(ii) in deinococcus radiodurans facilitates gamma-radiation resistance. | deinococcus radiodurans is extremely resistant to ionizing radiation. how this bacterium can grow under chronic gamma radiation [50 grays (gy) per hour] or recover from acute doses greater than 10 kgy is unknown. we show that d. radiodurans accumulates very high intracellular manganese and low iron levels compared with radiation-sensitive bacteria and that resistance exhibits a concentration-dependent response to manganous chloride [mn(ii)]. among the most radiation-resistant bacterial groups re ... | 2004 | 15459345 |
| the outer membrane cytochromes of shewanella oneidensis mr-1 are lipoproteins. | to determine if the outer membrane (om) cytochromes omca and omcb of the metal-reducing bacterium shewanella oneidensis mr-1 are lipoproteins, and to assess cell surface exposure of the cytochromes by radioiodination. | 2004 | 15482439 |
| arra is a reliable marker for as(v) respiration. | arsenate [as(v)]-respiring bacteria affect the speciation and mobilization of arsenic in the environment. this can lead to arsenic contamination of drinking water supplies and deleterious consequences for human health. using molecular genetics, we show that the functional gene for as(v) respiration, arra, is highly conserved; that it is required for as(v) reduction to arsenite when arsenic is sorbed onto iron minerals; and that it can be used to identify the presence and activity of as(v)-respir ... | 2004 | 15486292 |
| structure of 2c-methyl-d-erythritol-2,4-cyclodiphosphate synthase from shewanella oneidensis at 1.6 a: identification of farnesyl pyrophosphate trapped in a hydrophobic cavity. | isopentenyl pyrophosphate (ipp) is a universal building block for the ubiquitous isoprenoids that are essential to all organisms. the enzymes of the non-mevalonate pathway for ipp synthesis, which is unique to many pathogenic bacteria, have recently been explored as targets for antibiotic development. several crystal structures of 2c-methyl-d-erythritol-2,4-cyclophosphate (mecdp) synthase, the fifth of seven enzymes involved in the non-mevalonate pathway for synthesis of ipp, have been reported; ... | 2004 | 15502301 |
| global transcriptome analysis of the heat shock response of shewanella oneidensis. | shewanella oneidensis is an important model organism for bioremediation studies because of its diverse respiratory capabilities. however, the genetic basis and regulatory mechanisms underlying the ability of s. oneidensis to survive and adapt to various environmentally relevant stresses is poorly understood. to define this organism's molecular response to elevated growth temperatures, temporal gene expression profiles were examined in cells subjected to heat stress by using whole-genome dna micr ... | 2004 | 15516594 |
| dissimilatory fe(iii) and mn(iv) reduction. | dissimilatory fe(iii) and mn(iv) reduction has an important influence on the geochemistry of modern environments, and fe(iii)-reducing microorganisms, most notably those in the geobacteraceae family, can play an important role in the bioremediation of subsurface environments contaminated with organic or metal contaminants. microorganisms with the capacity to conserve energy from fe(iii) and mn(iv) reduction are phylogenetically dispersed throughout the bacteria and archaea. the ability to oxidiz ... | 2004 | 15518832 |
| microbial profiles of commercial, vacuum-packaged, fresh pork of normal or short storage life. | the microbial ecology of fresh vacuum-packed pork cuts during storage at -1.5 degrees c for up to 45 days was examined to characterize rates of microbial growth and ph changes in commercially prepared products of normal storage quality. pork loins in commercial distribution with odour defects were also studied to determine a possible cause of the defects and avoid future problems. in addition, microbial profiles of pork cuts from two plants were compared, after storage for 25 days at -1.5 degree ... | 2004 | 15527918 |
| survival of shewanella oneidensis mr-1 after uv radiation exposure. | we systematically investigated the physiological response as well as dna damage repair and damage tolerance in shewanella oneidensis mr-1 following uvc, uvb, uva, and solar light exposure. mr-1 showed the highest uvc sensitivity among shewanella strains examined, with d37 and d10 values of 5.6 and 16.5% of escherichia coli k-12 values. stationary cells did not show an increased uva resistance compared to exponential-phase cells; instead, they were more sensitive at high uva dose. uva-irradiated ... | 2004 | 15528503 |
| shewanella putrefaciens in a fuel-in-water emulsion from the prestige oil spill. | microorganisms that colonize the fuel-in-water emulsion from the prestige spill have been compared with those from exxon-valdez. both emulsions contained non-fermentative gram-negative rods but unlike exxon-valdez's, the prestige's spill contained anaerobic bacteria and no fungi. our main finding has been the identification of shewanella putrefaciens , a bacterium promising for bioremediation. | 2004 | 15539931 |
| shewanella profunda sp. nov., isolated from deep marine sediment of the nankai trough. | a novel piezotolerant, mesophilic, facultatively anaerobic, organotrophic, polarly flagellated bacterium (strain lt13a(t)) was isolated from a deep sediment layer in the nankai trough (leg 190, ocean drilling program) off the coast of japan. this organism used a wide range of organic substrates as sole carbon and energy sources: pyruvate, glutamate, succinate, fumarate, lactate, citrate, peptone and tryptone. oxygen, nitrate, fumarate, ferric iron and cystine were used as electron acceptors. max ... | 2004 | 15545415 |
| shewanella marisflavi sp. nov. and shewanella aquimarina sp. nov., slightly halophilic organisms isolated from sea water of the yellow sea in korea. | two gram-negative, motile, non-spore-forming, rod-shaped organisms, strains sw-117(t) and sw-120(t), were isolated from sea water of the yellow sea in korea and subjected to a polyphasic taxonomic study. strains sw-117(t) and sw-120(t) simultaneously contained both menaquinones (mk) and ubiquinones (q) as isoprenoid quinones; the predominant menaquinone was mk-7 and the predominant ubiquinones were q-7 and q-8. the major fatty acid detected in the two strains was iso-c(15 : 0). the dna g+c conte ... | 2004 | 15545482 |
| initial phases of biofilm formation in shewanella oneidensis mr-1. | shewanella oneidensis mr-1 is a facultative fe(iii)- and mn(iv)-reducing microorganism and serves as a model for studying microbially induced dissolution of fe or mn oxide minerals as well as biogeochemical cycles. in soil and sediment environments, s. oneidensis biofilms form on mineral surfaces and are critical for mediating the metabolic interaction between this microbe and insoluble metal oxide phases. in order to develop an understanding of the molecular basis of biofilm formation, we inves ... | 2004 | 15547283 |
| widespread presence of "bacterial-like" ppp phosphatases in eukaryotes. | in eukaryotes, ppp (protein phosphatase p) family is one of the two known protein phosphatase families specific for ser and thr. the role of ppp phosphatases in multiple signaling pathways in eukaryotic cell has been extensively studied. unlike eukaryotic ppp phosphatases, bacterial members of the family have broad substrate specificity or may even be tyr-specific. moreover, one group of bacterial ppps are diadenosine tetraphosphatases, indicating that bacterial ppp phosphatases may not necessar ... | 2004 | 15555063 |
| characterization of the eicosapentaenoic acid biosynthesis gene cluster from shewanella sp. strain scrc-2738. | the 38 kb eicosapentaenoic acid (epa) biosynthesis gene cluster of shewanella sp. strain scrc-2738 was cloned into the cosmid vector (pepa). a 27 kb nucleotide sequence of the xhoi to spei region of pepa showed epa production (6.3%) in e. coli jm109. among the nine open reading frames (orfs) in this sequence, only five (orfs 2 and 5-8) were essential for epa production. high levels of production (16%-22%) were found in e. coli jm109 transformed with a multicopy pneb vector carrying only the five ... | 2004 | 15565743 |
| transcriptomic and proteomic characterization of the fur modulon in the metal-reducing bacterium shewanella oneidensis. | the availability of the complete genome sequence for shewanella oneidensis mr-1 has permitted a comprehensive characterization of the ferric uptake regulator (fur) modulon in this dissimilatory metal-reducing bacterium. we have employed targeted gene mutagenesis, dna microarrays, proteomic analysis using liquid chromatography-mass spectrometry, and computational motif discovery tools to define the s. oneidensis fur regulon. using this integrated approach, we identified nine probable operons (con ... | 2004 | 15576789 |
| redox behaviour of the haem domain of flavocytochrome c3 from shewanella frigidimarina probed by nmr. | flavocytochrome c3 from shewanella frigidimarina (fcc3) is a tetrahaem periplasmic protein of 64 kda with fumarate reductase activity. this work reports the first example of nmr techniques applied to the assignment of the thermodynamic order of oxidation of the four individual haems for such large protein, expanding its applicability to a wide range of proteins. nmr data from partially and fully oxidised samples of fcc3 and a mutated protein with an axial ligand of haem iv replaced by alanine we ... | 2004 | 15581639 |
| cold-active dnak of an antarctic psychrotroph shewanella sp. ac10 supporting the growth of dnak-null mutant of escherichia coli at cold temperatures. | shewanella sp. ac10 is a psychrotrophic bacterium isolated from the antarctica that actively grows at such low temperatures as 0 degrees c. immunoblot analyses showed that a heat-shock protein dnak is inducibly formed by the bacterium at 24 degrees c, which is much lower than the temperatures causing heat shock in mesophiles such as escherichia coli. we found that the shewanella dnak (shednak) shows much higher atpase activity at low temperatures than the dnak of e. coli (ecodnak): a characteris ... | 2004 | 15599780 |
| polymicrobial bacteremia caused by escherichia coli, edwardsiella tarda, and shewanella putrefaciens. | edwardsiella tarda, a member of enterobacteriaceae, is found in freshwater and marine environments and in animals living in these environments. this bacterium is primarily associated with gastrointestinal diseases, and has been isolated from stool specimens obtained from persons with or without clinical infectious diseases. shewanella putrefaciens, a saprophytic gram-negative rod, is rarely responsible for clinical syndromes in humans. debilitated status and exposure to aquatic environments are ... | 2004 | 15605912 |
| actinide and metal toxicity to prospective bioremediation bacteria. | bacteria may be beneficial for alleviating actinide contaminant migration through processes such as bioaccumulation or metal reduction. however, sites with radioactive contamination often contain multiple additional contaminants, including metals and organic chelators. bacteria-based bioremediation requires that the microorganism functions in the presence of the target contaminant, as well as other contaminants. here, we evaluate the toxicity of actinides, metals and chelators to two different b ... | 2005 | 15643939 |
| targeted comparative proteomics by liquid chromatography-tandem fourier ion cyclotron resonance mass spectrometry. | in proteomics, effective methods are needed for identifying the relatively limited subset of proteins displaying significant changes in abundance between two samples. one way to accomplish this task is to target for identification by ms/ms only the "interesting" proteins based on the abundance ratio of isotopically labeled pairs of peptides. we have developed the software and hardware tools for online lc-fticr ms/ms studies in which a set of initially unidentified peptides from a proteome analys ... | 2005 | 15649034 |
| shewanella decolorationis sp. nov., a dye-decolorizing bacterium isolated from activated sludge of a waste-water treatment plant. | a highly efficient dye-decolorizing bacterium, strain s12t, was isolated from activated sludge of a textile-printing waste-water treatment plant in guangzhou, china. the cells were gram-negative and motile by means of a single polar flagellum. the strain was capable of anaerobic growth either by fermentation of glucose or by anaerobic respiration and utilized a variety of electron acceptors, including nitrate, iron oxide and thiosulfate. the physiological properties, tested by using the biolog g ... | 2005 | 15653901 |
| induction of rapid detachment in shewanella oneidensis mr-1 biofilms. | active detachment of cells from microbial biofilms is a critical yet poorly understood step in biofilm development. we discovered that detachment of cells from biofilms of shewanella oneidensis mr-1 can be induced by arresting the medium flow in a hydrodynamic biofilm system. induction of detachment was rapid, and substantial biofilm dispersal started as soon as 5 min after the stop of flow. we developed a confocal laser scanning microscopy-based assay to quantify detachment. the extent of bioma ... | 2005 | 15659679 |
| validation of shewanella oneidensis mr-1 small proteins by amt tag-based proteome analysis. | using stringent criteria for protein identification by accurate mass and time (amt) tag mass spectrometric methodology, we detected 36 proteins of <101 amino acids in length, including 10 that were annotated as hypothetical proteins, in 172 global tryptic digests of shewanella oneidensis mr-1 proteins. peptides that map to the conserved, but functionally uncharacterized proteins so4134 and so2787, were the most frequently detected peptides in these samples, while those that map to hypotheticals ... | 2004 | 15669716 |
| stabilities and activities of the n- and c-domains of fkbp22 from a psychrotrophic bacterium overproduced in escherichia coli. | fkbp22 from a psychrotrophic bacterium shewanella sp. sib1, is a dimeric protein with peptidyl prolyl cis-trans isomerase (ppiase) activity. according to homology modeling, it consists of an n-terminal domain, which is involved in dimerization of the protein, and a c-terminal catalytic domain. a long alpha3 helix spans these domains. an n-domain with the entire alpha3 helix (n-domain+) and a c-domain with the entire alpha3 helix (c-domain+) were overproduced in escherichia coli in a his-tagged f ... | 2005 | 15670146 |
| bioreductive deposition of palladium (0) nanoparticles on shewanella oneidensis with catalytic activity towards reductive dechlorination of polychlorinated biphenyls. | microbial reduction of soluble pd(ii) by cells of shewanella oneidensis mr-1 and of an autoaggregating mutant (coag) resulted in precipitation of palladium pd(0) nanoparticles on the cell wall and inside the periplasmic space (biopd). as a result of biosorption and subsequent bioreduction of pd(ii) with h2, formate, lactate, pyruvate or ethanol as electron donors, recoveries higher than 90% of pd associated with biomass could be obtained. the biopd(0) nanoparticles thus obtained had the ability ... | 2005 | 15683392 |
| global profiling of shewanella oneidensis mr-1: expression of hypothetical genes and improved functional annotations. | the gamma-proteobacterium shewanella oneidensis strain mr-1 is a metabolically versatile organism that can reduce a wide range of organic compounds, metal ions, and radionuclides. similar to most other sequenced organisms, approximately 40% of the predicted orfs in the s. oneidensis genome were annotated as uncharacterized "hypothetical" genes. we implemented an integrative approach by using experimental and computational analyses to provide more detailed insight into gene function. global expre ... | 2005 | 15684069 |
| low-temperature growth of shewanella oneidensis mr-1. | shewanella oneidensis mr-1 is a mesophilic bacterium with a maximum growth temperature of approximately 35 degrees c but the ability to grow over a wide range of temperatures, including temperatures near zero. at room temperature ( approximately 22 degrees c) mr-1 grows with a doubling time of about 40 min, but when moved from 22 degrees c to 3 degrees c, mr-1 cells display a very long lag phase of more than 100 h followed by very slow growth, with a doubling time of approximately 67 h. in compa ... | 2005 | 15691935 |
| [infectious cellulitis and shewanella alga septicemia in an immunocompetent patient]. | shewanella alga is a gram-negative bacilla often found in water or soil. clinical infections in humans are rare, with serious infections described generally in immunocompromised hosts. | 2004 | 15692446 |
| structural domains, protein modules, and sequence similarities enrich our understanding of the shewanella oneidensis mr-1 proteome. | the protein coding sequences of s. oneidensis mr-1 were analyzed, and new annotations were given to 491 gene products, 306 of which were previously of unknown function. new information was mainly brought in from structural domain predictions for s. oneidensis proteins of the superfam database (http://supfam.mrc-lmb.cam.ac.uk/superfamily/) and newly identified and experimentally verified functions of homologous proteins. proteins encoded by fused genes were identified and separated into modules, ... | 2004 | 15703478 |
| proteomics of shewanella oneidensis mr-1 biofilm reveals differentially expressed proteins, including agga and ribb. | shewanella oneidensis mr-1 is a gram-negative, facultative aerobic bacterium, able to respire a variety of electron acceptors. due to its capability to reduce solid ferric iron, s. oneidensis plays an important role in microbially induced corrosion of metal surfaces. since this requires cellular adhesion to the metal surface, biofilm growth is an essential feature of this process. the goal of this work was to compare the global protein expression patterns of sessile and planktonic grown s. oneid ... | 2005 | 15712242 |
| crystal structure of cold-active protein-tyrosine phosphatase from a psychrophile, shewanella sp. | the cold-active protein-tyrosine phosphatase (captpase) of a psychrophile, shewanella sp., shows high catalytic activity below 20 degrees c. the catalytic residue of captpase is histidine, as opposed to the cysteine of known protein-tyrosine phosphatases (ptpases), and the enzyme protein has three amino acid sequences, asp-xaa-his, gly-asp-xaa-xaa-asp-arg and gly-asn-his-glu, that are observed in many protein-serine/threonine phosphatases (ps/tpases). we have determined the crystal structures of ... | 2005 | 15713885 |
| gene cloning, overproduction, and characterization of thermolabile alkaline phosphatase from a psychrotrophic bacterium. | the gene encoding alkaline phosphatase from the psychrotrophic bacterium shewanella sp. sib1 was cloned, sequenced, and overexpressed in escherichia coli. the recombinant protein was purified and its enzymatic properties were compared with those of e. coli alkaline phosphatase (apase), which shows an amino acid sequence identity of 37%. the optimum temperature of sib1 apase was 50 degrees c, lower than that of e. coli apase by 30 degrees c. the specific activity of sib1 apase at 50 degrees c was ... | 2005 | 15725663 |
| overexpression of multi-heme c-type cytochromes. | | 2005 | 15727136 |
| application of flow field flow fractionation-icpms for the study of uranium binding in bacterial cell suspensions. | field flow fractionation (fff) is a size-based separation technique applicable to biomolecules, colloids, and bacteria in solution. when interfaced with icpms on-line, elemental data can be collected concurrent with size distribution. we employed hyperlayer flow fff (fl fff) methodology to separate cells of shewanella oneidensis strain mr-1 from exopolymers present in washed cell suspensions. with a channel flow of 4 ml min-1 and a cross-flow of 0.4 ml min-1 cells eluted with a retention time of ... | 2005 | 15732923 |
| electricity generation from cysteine in a microbial fuel cell. | in a microbial fuel cell (mfc), power can be generated from the oxidation of organic matter by bacteria at the anode, with reduction of oxygen at the cathode. proton exchange membranes used in mfcs are permeable to oxygen, resulting in the diffusion of oxygen into the anode chamber. this could either lower power generation by obligate anaerobes or result in the loss in electron donor from aerobic respiration by facultative or other aerobic bacteria. in order to maintain anaerobic conditions in c ... | 2005 | 15743641 |
| evaluation of candidate probiotic strains for gilthead sea bream larvae (sparus aurata) using an in vivo approach. | the aim of this study was to evaluate the effect of six bacterial strains on gilthead sea bream larvae (sparus aurata). | 2005 | 15752217 |
| genetic and biochemical characterization of the chromosome-encoded class b beta-lactamases from shewanella livingstonensis (slb-1) and shewanella frigidimarina (sfb-1). | to determine the beta-lactamase gene content of beta-lactam-susceptible psychrophilic gram-negative bacilli, shewanella frigidimarina and shewanella livingstonensis, isolated from a marine environment. | 2005 | 15772146 |
| transcriptome analysis of shewanella oneidensis mr-1 in response to elevated salt conditions. | whole-genomic expression patterns were examined in shewanella oneidensis cells exposed to elevated sodium chloride. genes involved in na(+) extrusion and glutamate biosynthesis were significantly up-regulated, and the majority of chemotaxis/motility-related genes were significantly down-regulated. the data also suggested an important role for metabolic adjustment in salt stress adaptation in s. oneidensis. | 2005 | 15774893 |
| a new method for c-terminal sequence analysis in the proteomic era. | the overall study of post-translational modifications (ptms) of proteins is gaining strong interest. beside phosphorylation and glycosylation, truncations of the nascent polypeptide chain at the amino or carboxy terminus are by far the most common types of ptms in proteins. in contrast to the analysis of phosphorylation and glycosylation sites, relatively little attention has been paid to the development of approaches for the systematic analysis of proteolytic processing events. here we present ... | 2005 | 15782188 |
| identification of rpobc genes encoding for beta and beta' subunits of rna polymerase in a deep-sea piezophilic bacterium, shewanella violacea strain dss12. | rna polymerase from cells of the deep-sea bacterium shewanella violacea dss12 was purified using three chromatographic steps. an in vitro transcription assay indicated that the purified enzyme was sigma(70) containing rna polymerase. the enzyme activity was inhibited in the presence of rifampicin when the sensitive domain was targeted. the rpobc genes encoding for the beta and beta' subunits of rna polymerase were cloned and their nucleotide sequences determined. expression plasmids, designated ... | 2005 | 15784987 |
| how radiation kills cells: survival of deinococcus radiodurans and shewanella oneidensis under oxidative stress. | we have recently shown that deinococcus radiodurans and other radiation resistant bacteria accumulate exceptionally high intracellular manganese and low iron levels. in comparison, the dissimilatory metal-reducing bacterium shewanella oneidensis accumulates fe but not mn and is extremely sensitive to radiation. we have proposed that for fe-rich, mn-poor cells killed at radiation doses which cause very little dna damage, cell death might be induced by the release of fe(ii) from proteins during ir ... | 2005 | 15808748 |
| nonbioluminescent strains of photobacterium phosphoreum produce the cell-to-cell communication signal n-(3-hydroxyoctanoyl)homoserine lactone. | bioluminescence is a common phenotype in marine bacteria, such as vibrio and photobacterium species, and can be quorum regulated by n-acylated homoserine lactones (ahls). we extracted a molecule that induced a bacterial ahl monitor (agrobacterium tumefaciens nt1 [pzlr4]) from packed cod fillets, which spoil due to growth of photobacterium phosphoreum. interestingly, ahls were produced by 13 nonbioluminescent strains of p. phosphoreum isolated from the product. of 177 strains of p. phosphoreum (i ... | 2005 | 15812045 |
| shewanella algae and shewanella putrefaciens: clinical and microbiological characteristics. | the occurrence of the two shewanella species found in clinical specimens, shewanella algae and shewanella putrefaciens, correlates with the temperature and salinity of seawater. this means that shewanella infections occur in warm climates or during especially warm summers in temperate climates. the infections described most commonly involve ears, skin and soft tissue, with or without bacteraemia. primary bacteraemia with a fulminant course is also seen in immunocompromised patients. important di ... | 2005 | 15819859 |
| monochloramine versus sodium hypochlorite as antimicrobial agents for reducing populations of bacteria on broiler chicken carcasses. | studies were conducted to compare the effect of sodium hypochlorite (sh) versus monochloramine (mon) on bacterial populations associated with broiler chicken carcasses. in study 1, nominal populations (6.5 to 7.5 log cfu) of escherichia coli, listeria monocytogenes, pseudomonas fluorescens, salmonella serovars, shewanella putrefaciens, and staphylococcus aureus were exposed to sterilized chiller water (controls) or sterilized chiller water containing 50 ppm sh or mon. sh at 50 ppm eliminated all ... | 2005 | 15830667 |
| enhancement of polyunsaturated fatty acid production by cerulenin treatment in polyunsaturated fatty acid-producing bacteria. | when docosahexaenoic acid (dha)-producing moritella marina strain mp-1 was cultured in the medium containing 0.5 microg cerulenin ml-1, an inhibitor for fatty acid biosynthesis, the cells grew normally, but the content of dha in the total fatty acids increased from 5.9-19.4%. the dha yield of m. marina strain mp-1 cells also increased from 4 to 13.7 mg l-1 by cerulenin treatment. the same effect of cerulenin was observed in eicosapentaenoic acid (epa)-producing shewanella marinintestina strain i ... | 2005 | 15834803 |
| pressure-regulated biosynthesis of cytochrome bd in piezo- and psychrophilic deep-sea bacterium shewanella violacea dss12. | the genes of cytochrome bd-encoding cydab were identified from a deep-sea bacterium shewanella violacea dss12. these showed significant homologies with known cydab gene sequences from various organisms. additionally, highly conserved regions that are important for the enzymatic function were also conserved in cyda of s. violacea. based on the results, transcriptional analysis of cydab operon and cyddc operon (required for assembly of cytochrome bd) of s. violacea in microaerobic condition was pe ... | 2005 | 15844013 |
| respiration and growth of shewanella oneidensis mr-1 using vanadate as the sole electron acceptor. | shewanella oneidensis mr-1 is a free-living gram-negative gamma-proteobacterium that is able to use a large number of oxidizing molecules, including fumarate, nitrate, dimethyl sulfoxide, trimethylamine n-oxide, nitrite, and insoluble iron and manganese oxides, to drive anaerobic respiration. here we show that s. oneidensis mr-1 is able to grow on vanadate as the sole electron acceptor. oxidant pulse experiments demonstrated that proton translocation across the cytoplasmic membrane occurs during ... | 2005 | 15866913 |
| comparative analysis of differentially expressed genes in shewanella oneidensis mr-1 following exposure to uvc, uvb, and uva radiation. | we previously reported that shewanella oneidensis mr-1 is highly sensitive to uvc (254 nm), uvb (290 to 320 nm), and uva (320 to 400 nm). here we delineated the cellular response of mr-1 to uv radiation damage by analyzing the transcriptional profile during a 1-h recovering period after uvc, uvb, and uva exposure at a dose that yields about a 20% survival rate. although the sos response was observed with all three treatments, the induction was more robust in response to short-wavelength uv radia ... | 2005 | 15866945 |
| anaerobic regulation by an atypical arc system in shewanella oneidensis. | shewanella oneidensis strain mr-1 is well known for its respiratory versatility, yet little is understood about how it regulates genes involved in anaerobic respiration. the arc two-component system plays an important role in this process in escherichia coli; therefore, we determined its function in s. oneidensis. arca from s. oneidensis complements an e. coli arca mutant, but the arc regulon in s. oneidensis constitutes a different suite of genes. for example, one of the strongest arca-regulate ... | 2005 | 15882425 |
| structure of the o-specific polysaccharide from shewanella japonica type strain kmm 3299t containing the rare amino sugar fuc4nac. | an acidic o-specific polysaccharide (ps) of the agar-digesting bacterium shewanella japonica with the type strain kmm 3299(t) was obtained by mild acid hydrolysis of the lipopolysaccharide. the polysaccharide was studied by component analysis, methylation analysis, (1)h and (13)c nmr spectroscopy, including 2d nmr experiments. the ps was determined to have the following structure involving three unusual amino sugars: | 2005 | 15882857 |
| automated 20 kpsi rplc-ms and ms/ms with chromatographic peak capacities of 1000-1500 and capabilities in proteomics and metabolomics. | proteomics analysis based-on reversed-phase liquid chromatography (rplc) is widely practiced; however, variations providing cutting-edge rplc performance have generally not been adopted even though their benefits are well established. here, we describe an automated format 20 kpsi rplc system for proteomics and metabolomics that includes on-line coupling of micro-solid phase extraction for sample loading and allows electrospray ionization emitters to be readily replaced. the system uses 50 microm ... | 2005 | 15889897 |
| microbiological quality changes in the intestine of hybrid tilapia (oreochromis niloticus x oreochromis aureus) in fresh and frozen storage condition. | the goal of this study was to monitor the quantitative and qualitative bacterial flora in the intestine of hybrid tilapia in fresh fish and fish kept in frozen storage conditions for 1 year. | 2005 | 15892747 |
| surface structure and nanomechanical properties of shewanella putrefaciens bacteria at two ph values (4 and 10) determined by atomic force microscopy. | the nanomechanical properties of gram-negative bacteria (shewanella putrefaciens) were investigated in situ in aqueous solutions at two ph values, specifically, 4 and 10, by atomic force microscopy (afm). for both ph values, the approach force curves exhibited subsequent nonlinear and linear regimens that were related to the progressive indentation of the afm tip in the bacterial cell wall, including a priori polymeric fringe (nonlinear part), while the linear part was ascribed to compression of ... | 2005 | 15901713 |
| [opportunistic infections caused by shewanella, new emergent bacteria]. | shewanella putrefaciens and shewanella algae are gram negative, nonfermentative and oxidative bacilli whose the main phenotypic feature is the production of hydrogen sulfide gas. widespread in the environment, both s. putrefaciens and s. algae species are rare human bacteria although they are reported with increasing frequency as a cause of opportunistic infection in humans, such as skin and soft tissue infections and bacteremia. chronic infections of the lower limbs and liver disease have been ... | 2005 | 15914286 |
| the structure of the capsular polysaccharide of shewanella oneidensis strain mr-4. | capsular polysaccharides were extracted from shewanella oneidensis strain mr-4, grown on two different culture media. the polysaccharides were analyzed using 1h and 13c nmr spectroscopy, and the following structure of the repeating unit was established: [structure: see text] where the residue of 4-amino-4,6-dideoxy-d-glucose (qui4n) was substituted with different n-acyl groups depending on the growth media. all monosaccharides are present in the pyranose form. in the ps from cells grown on enric ... | 2005 | 15936738 |
| nmr methods for in situ biofilm metabolism studies. | novel procedures and instrumentation are described for nuclear magnetic resonance (nmr) spectroscopy and imaging studies of live, in situ microbial films. a perfused nmr/optical microscope sample chamber containing a planar biofilm support was integrated into a recirculation/dilution flow loop growth reactor system and used to grow in situ shewanella oneidensis strain mr-1 biofilms. localized nmr techniques were developed and used to non-invasively monitor time-resolved metabolite concentrations ... | 2005 | 15936835 |
| de novo sequence analysis of n-terminal sulfonated peptides after in-gel guanidination. | here we report a novel approach in which gel-separated proteins are guanidinated in-gel prior to enzymatic cleavage. in contrast to previously described techniques, this procedure allows the extracted tryptic peptides to be n-terminal sulfonated without any further sample purification. the derivatized peptides were subsequently fragmented using a matrix-assisted laser desorption/ionization time of flight/time of flight instrument. the approach facilitates the de novo sequence analysis and allows ... | 2005 | 15937995 |