| a bewitching case of factitious disorder in zimbabwe. | a rural zimbabwean man attributed the appearance of needles in his leg to witchcraft, but medical practitioners suspected that the needles were self-introduced. psychiatric evaluation revealed no major mental illness. the patient met criteria for the euro-american diagnosis of factitious disorder, persisting in his claim that he had been the victim of witchcraft. the patient's claim cannot be considered delusional because belief in witchcraft is culturally sanctioned within shona culture. the ca ... | 1996 | 8937912 |
| impaired bronchial healing after lung donation from non-heart-beating donors. | bronchial viability after lung transplantation remains a concern. modern preservation methods, surgical technique, and limited cold ischemic periods have decreased the frequency of bronchial complications. however, lungs procured from non-heart-beating donors are subjected to a mandatory period of warm ischemia. we investigated bronchial healing in a porcine survival model of left lung transplantation using organ procurement from non-heart-beating donors after a 60-minute period of warm ischemia ... | 1996 | 8956117 |
| protective effect of uw solution on postischemic injury in rat liver: suppression of reduction in hepatic antioxidants during reperfusion. | preservation with university of wisconsin (uw) solution can maintain liver graft function and produces survival rates of recipients higher than that with euro collins (ec) solution. to explore the underlying mechanisms, we transplanted rat livers following cold preservation with ec or uw solution for 18 hr, and measured hepatic adenine nucleotide levels, the percentage of water content, lactate levels, and endogenous antioxidant levels (alpha-tocopherol [alpha-toc], reduced coenzyme q9 [coq9h2], ... | 1996 | 8958262 |
| hepatic resuscitation after warm anoxia: one approach for increasing the donor pool for liver transplantation. | the deleterious effects of warm anoxia on the liver are seen to be irreversible if cooling and transplantation (lt) follow immediately after. the aim of our study is to demonstrate that livers subjected to anoxia may be suitable for lt if a period of resuscitation is interposed before the cooling process. forty female large white pigs were used. preservation (euro-collins solution) and lt technique were the same in all 20 procedures. all donors underwent clamping of the porta hepatis at the end ... | 1996 | 8959807 |
| the importance of static lung inflation during organ storage: the impact of varying ischemic intervals in a double lung rat transplantation model. | to determine the importance of static lung inflation during storage, graft performance was evaluated at different levels of intratracheal pressure and varying ischemic intervals. lewis rat lungs were perfused with low-potassium euro-collins and stored for 4 or 8 hr either in atelectasis (4 hr: group i; 8 hr: group iv, respectively) or 13 (group ii; v) or 26 cmh2o of airway pressure (groups iii, vi). following implantation continuous measurement of alveolar-arterial oxygen difference (aado2*) and ... | 1996 | 8990350 |
| toxic trace element reference levels in blood and urine: influence of gender and lifestyle factors. | this study is part of the euro-terviht project (trace element reference values in human tissues) which aims at establishing reference intervals for trace elements in blood, urine and other human tissues. in this study reference intervals (0.05-0.95 fractiles) were estimated for lead in blood (105-529 nmol/l for men, 80-340 nmol/l for women), manganese in blood (100-271 nmol/l) and arsenic in urine (36-541 nmol/l for men, 21-475 nmol/l for women). upper reference limits (0.95 fractile) were estab ... | 1997 | 9301099 |
| both blood and crystalloid-based extracellular solutions are superior to intracellular solutions for lung preservation. | lung transplantation remains limited by donor organ ischemic time, inadequate graft preservation, and reperfusion injury. we evaluated lung preservation with use of an extracellular solution, with or without the addition of blood, as compared with preservation with the intracellular euro-collins solution. | 1996 | 8975843 |
| adenine nucleotide metabolism of canine pancreas graft preserved by the two-layer (euro-collins' solution/perfluorochemical) method after reperfusion. | we have demonstrated that oxygenation of a pancreas during preservation by the two-layer method leads continued atp production to maintain cellular integrity and produces an extended period of preserved pancreatic viability. in this study, we examined whether the pancreas grafts preserved by the two-layer method maintained adenine nucleotides pool as substrates for atp synthesis before reperfusion and had the ability to synthesize atp promptly and to recover energy charge potential (ecp) of the ... | 1996 | 8984228 |
| [proton magnetic resonance spectroscopy of excised pig liver]. | after evaluating ex vivo pig liver by 1h magnetic resonance spectroscopy (1h-mrs) using a 1.5 tesla super-conducting unit, the assignment of peaks was reevaluated in vitro using a 9.4 tesla superconducting unit. the portal vein was cannulated and perfused by cooled euro-collins solution, and pig liver was removed and preserved in the solution. five to 8 g of the liver was excised before and after preservation, then extracted by perchloric acid (pca). in 1h-mrs of pca extracted pig liver, large p ... | 1996 | 8992454 |
| improved distribution of pulmonary flush solution to the tracheobronchial wall in pulmonary transplantation. | this report describes the experimental results obtained with conventional (pulmonary artery, pa) flushing versus retrograde perfusion (via left atrium, la) using 99mtc-labeled macroaggregated albumin (maa-99mtc) to ascertain the distribution throughout the tracheobronchial (tb) tree in 10 large-white pigs. lung preservation was achieved with 4 degrees c euro-collins solution (60 ml/kg) instilled via pa (n = 5) or la (n = 5). simultaneously, maa-99mtc was given using the same respective route and ... | 1997 | 9013099 |
| functional and biochemical evaluation of the preserved lung in a rat model. | lactate dehydrogenase (ldh) and aspartate aminotransferase (ast) activities and total protein concentrations were examined in the postrinsing solutions from rat lungs preserved in phosphate-buffered saline (pbs), euro-collins (e-c) solution, or low-potassium e-c solution for 8 h at 4 degrees c, 10 degrees c, or 20 degrees c. the ldh and ast activities were higher when the organs were preserved in pbs for 8 h at 20 degrees c than at 4 degrees c or 10 degrees c, while the total protein concentrati ... | 1996 | 9017970 |
| addition of calcium to euro-collins solution is essential for 24-hour preservation of the vasculature. | genuine euro-collins solution is calcium free. the aim of this study was to investigate whether the addition of calcium would improve its capacity to preserve the vasculature. | 1997 | 9033310 |
| [role of membrane protectors and antioxidants in the process of perfusion and preservation of donor lungs]. | comparative study of the effect of four perfusion and preservation solutions on lung function, conducted in experiments on 69 inbred dogs, revealed the following regular features. euro-collins and electrolyte solutions cause a marked increase of the capillary-hydrostatic pressure, a significant decrease of colloido-osmotic pressure of the plasma, and, as a result, reduction of the ient between them. this is manifested by edema of the lungs. the use of lpd solution is attended by moderate edema o ... | 1997 | 9162773 |
| evaluation of normothermic ischemia and simple cold preservation injury in pig kidney by proton nuclear magnetic resonance spectroscopy. | the isolated perfused pig kidney (ipk) model was used to mimic the non-heart-beating donor situation. this model was performed to assess initial renal functions after normothermic ischemia, cold flush, and 24 hr cold-storage preservation (csp) with euro-collins and to determine normothermic ischemia and reperfusion impairment by biochemical, histological, and proton nuclear magnetic resonance (nmr) spectroscopy analysis. twenty-four pig kidneys were used. there were three experimental groups: gr ... | 1997 | 9184669 |
| annual congress of the european association for endoscopic surgery; incorporated in the 1st joint euro-asian congress for endoscopic surgery. istanbul, turkey, 17-21 june 1997. abstracts. | | 1997 | 9244988 |
| the determination of membrane permeability coefficients of canine pancreatic islet cells and their application to islet cryopreservation. | sufficient numbers of pancreatic islets for successful allotransplantation can be achieved by storing and then pooling islets from several donors. optimal mhc matching and infectious disease screening also require long-term storage of islets, and cryopreservation is currently the only practical approach. cryopreservation protocols may be optimized by modeling the changes in cell volume and the associated damage incurred during cryoprotectant addition and dilution and during cooling and warming. ... | 1997 | 9245505 |
| hepatoprotective effect of a nonselective endothelin receptor antagonist (tak-044) in the transplanted liver. | this study was designed to investigate whether or not a novel nonselective endothelin a/b (eta/etb) receptor antagonist (tak-044) provides hepatoprotection during porcine liver transplantation. the grafts were stored in chilled euro-collins solution and recirculated following reflush with lactated ringer's with (tak group) or without (control group) tak-044 (10 mg/kg). intracellular (cytoplasma, mitochondria, and nucleus) calcium (ca) concentrations were measured in the hepatic biopsy materials ... | 1997 | 9245565 |
| extensive nucleotide changes and deletions within the envelope glycoprotein gene of euro-african west nile viruses. | we compared the sequence of an envelope protein gene fragment from 21 temporally distinct west nile (wn) virus strains, isolated in nine african countries and in france. alignment of nucleotide sequences defined two groups of viruses which diverged by up to 29%. the first group of subtypes is composed of nine wn strains from france and africa. the austral-asian kunjin virus was classified as a wn subtype in this first group. the second group includes 12 wn strains from africa and madagascar. fou ... | 1997 | 9292017 |
| effect of prolonged hypothermic ischemia and reperfusion on oxygen consumption and total mechanical energy in rat myocardium: participation of mitochondrial oxidative phosphorylation. | to reduce ischemia-reperfusion injury of hearts in open heart surgery and transplantation, it is important to know the critical period of ischemia in which donor hearts can sustain their function satisfactorily. cardiac function has been deduced from oxygen consumption (vo2) and mechanical parameters such as pressure-volume area (pva). inhibited mitochondrial oxidative phosphorylation during ischemia indicates that atp production is uncoupled from vo2. therefore, both mitochondrial oxidative pho ... | 1997 | 9293868 |
| prolongation of ischaemic tolerance of rat skeletal muscle by trehalose. | trehalose is a disaccharide that can stabilise cell membranes under various stressful conditions. we have studied the effect of trehalose on the ischaemic tolerance of fat skeletal muscle flaps. a rat gracilis model was used to evaluate the prolongation of ischaemic preservation of the modified solution in which trehalose was substituted for glucose in euro-collins solution. four groups of animals were studied. in two groups, flaps were preserved in euro-collins solution or trehalose euro-collin ... | 1997 | 9299680 |
| acellular low-potassium dextran preserves pulmonary function after 48 hours of ischemia. | we previously have shown that extracellular preservation solutions provide superior pulmonary protection after 18 hours of cold ischemia at 4 degrees c in an isolated, whole-blood-perfused, rabbit lung model. we also reported that the addition of 20% whole blood to a low-potassium dextran solution (blpd) conferred no discernible advantage over low-potassium dextran (lpd) alone in this same model. our current study was aimed at documenting the importance of blood in buffering extracellular preser ... | 1997 | 9307476 |
| potassium-reduced lung preservation solutions: a screening study. | in recent years several potassium-reduced solutions have been developed for improvement of pulmonary preservation. a comparison of these solutions in a single study, however, has not yet been performed. in an extracorporeal working rat heart-lung model (n = 7/group) lungs were preserved with 20 ml euro-collins (ec), low potassium ec (lpec), low potassium 2% dextran (lpd), et-kyoto (etk) or low potassium 5% dextran (perfadex) solution, while hearts were arrested with 10 ml st. thomas' cardioplegi ... | 1997 | 9323484 |
| accumulation of platelets in rat syngeneic lung transplants: a potential factor responsible for preservation-reperfusion injury. | platelets are known to play an important role in the pathogenesis of adult respiratory distress syndrome as well as preservation-reperfusion injury of liver allografts. however, the role of platelets in pulmonary preservation-reperfusion injury is unknown. in this study, we examined whether the extent of platelet accumulation in the preserved and subsequently reperfused lungs correlated with the degree of preservation-reperfusion injury in a rat lung isotransplant model. | 1997 | 9326401 |
| vimentin- and desmin-positive cells in the moulting budgerigar (melopsittacus undulatus) skin. | the distribution of vimentin- and desmin-positive cells in the budgerigar (melopsittacus undulatus) dermis was investigated by means of immunohistochemical reactivity with the commercially available (euro-diagnostics) polyclonal antibodies. the staining pattern for vimentin in the paraffin sections was generally comparable to that in other animal species with regard to endothelial cells, vascular wall cells, muscle cells and fibroblasts. the modified schwann cells in the inner core of the herbst ... | 1997 | 9334495 |
| reduced lipid peroxidation and ischemia-reperfusion injury after lung transplantation using low-potassium dextran solution for lung preservation. | ischemia-reperfusion injury is one of the significant problems in clinical lung transplantation. we investigated the effect of lung preservation with euro-collins solution (ec group) or low-potassium dextran solution (lpd group) on lipid peroxidation and ischemia-reperfusion injury in a pig model of lung allotransplantation. the donor lungs were preserved at 4 degrees c for 18 h. left-sided single lung transplantation was performed, followed by 6 h of reperfusion. lipid peroxidation was measured ... | 1997 | 9351605 |
| hepatic allograft procurement from non-heart-beating donors: limits of warm ischemia in porcine liver transplantation. | to investigate the tolerance to warm ischemia of liver grafts from non-heart-beating donors, porcine orthotopic liver transplantation was performed using grafts obtained at various periods after cardiac arrest. graft viability was investigated in relation to changes in hepatic adenine nucleotide metabolism. in donors, livers were divided into four groups according to warm ischemic time after cardiac arrest (group 1: 0 min, n=3; group 2: 30 min, n=3; group 3: 60 min, n=5; group 4: 90 min, n=4). t ... | 1997 | 9039925 |
| evaluation of injury preservation in pig kidney cold storage by proton nuclear magnetic resonance spectroscopy of urine. | the isolated perfused kidney (ipk) model from pig was used to assess initial renal functions after cold flush and a 24- and 48-hour cold storage preservation (csp) with euro-collins (ec) and to determine ischemia reperfusion (ir) impairment by biochemical, histological and proton nuclear magnetic resonance (nmr) spectroscopy of urine analysis. eighteen pig kidneys were used. there were three experimental groups: group 1--control kidneys flushed with cold heparinized saline, group 2--cold flush f ... | 1997 | 9072561 |
| hypothermic storage alone in lung preservation for transplantation: a metabolic, light microscopic, and functional analysis after 18 hours of preservation. | current lung preservation consists of flushing of the donor organs, with successive hypothermic storage in an inflated state. recently, hypothermic storage alone was reported to be superior in terms of functional recovery. this study was designed to investigate the metabolic, morphologic, and functional consequences of hypothermic storage alone, in experimental lung preservation. | 1997 | 9075828 |
| morphologic characteristics of human cervical pedicles. | cervical pedicle morphology was investigated using manual and computed tomography measurements. | 1997 | 9076880 |
| in vitro pulmonary arterial relaxation after experimental lung transplantation with extracorporeal circulation. | in search of an agent to attenuate the increased pulmonary vascular resistance after lung transplantation with extracorporeal circulation, we investigated contractions and relaxations of isolated porcine pulmonary arterial rings in organ chambers. | 1997 | 9080165 |
| antiapoptotic compound to enhance hypothermic liver preservation. | apoptosis (programmed cell death) occurs as a consequence of global organ ischemia during isolation and storage prior to transplantation. if apoptosis is inhibited during ischemia, organ preservation should be improved, and the length of time for permissible storage may be increased. the objective of this study was to test the effect of a newly developed antiapoptotic compound, lxr-015, during extended hypothermic liver preservation. | 1997 | 9089218 |
| a new organ preservation solution, celsior, is superior to euro-collins and university of wisconsin solutions in decreasing lung reperfusion injury. | | 1997 | 9123338 |
| inhaled nitric oxide at the time of harvest improves early lung allograft function. | inhalation of nitric oxide (no) has been shown to have beneficial effects on a variety of acute lung injuries, including lung allograft reperfusion injury. the purpose of the present study was to investigate the effects of inhaled no at the time of harvest on function of canine left lung allografts after transplantation. | 1997 | 9146331 |
| low-dose sodium nitroprusside reduces pulmonary reperfusion injury. | reperfusion injury is a significant cause of early allograft dysfunction after lung transplantation. we hypothesized that direct pulmonary arterial infusion of an intravascular nitric oxide donor, sodium nitroprusside (snp), would ameliorate pulmonary reperfusion injury more effectively than inhaled nitric oxide without causing profound systemic hypotension. | 1997 | 9146333 |
| effects of lung preservation with euro-collins and university of wisconsin solutions on endothelium-dependent relaxations. | this study compares the effect of lung preservation using flush perfusion of euro-collins or university of wisconsin solution on the pulmonary vascular function of endothelium-dependent and endothelium-independent relaxations. | 1997 | 9146338 |
| effects of blockade of kupffer cells by gadolinium chloride on hepatobiliary function in cold ischemia-reperfusion injury of rat liver. | the mechanisms of liver injury from cold storage and reperfusion are not completely understood. the aim of the present study was to investigate: 1) whether the inactivation of kupffer cells (kcs) by gadolinium chloride (gadcl) modulates cold ischemia-reperfusion injury of rat liver; and 2) whether cold storage of rat liver involves injury to biliary epithelial cells (becs). hepatobiliary function was assessed using an isolated perfused rat liver model. compared with control livers, in livers sub ... | 1997 | 9362369 |
| [the anatomy, surgical technic, preservation and reperfusion of the rat liver in an experiment]. | preservation of hepar of 86 rats using euro-collins' and bretschneider preservation solutions was realized in experiment. the animals hepar anatomy was studied thoroughly, the hepar preservation pattern was improved, the modified pattern of the hepar isolated perfusion was used. morphologic, cytologic and biochemical investigations were conducted to estimate the viability of hepar. | 1997 | 9377253 |
| [the effect of euro-collins' and euro-collins'+n preservation solutions on the course of a dystrophic process in the rat liver]. | the influence of euro-collins and euro-collins with the membrane stabilizing additives conserving solutions on the course of dystrophic process in the rat's hepatic parenchyma was studied. application of the membrane stabilizing additives promoted the hepatic structure stabilization, showing the reduce of the hydropic dystrophia intensity and extent. | 1997 | 9377254 |
| trimetazidine prevents renal injury in the isolated perfused pig kidney exposed to prolonged cold ischemia. | ischemia caused by cold storage (cs) and reperfusion of the kidney is often responsible for delayed graft function after transplantation. significant attention has been focused on the cascade of events involved in ischemia-reperfusion injury, with the objective of identifying drugs to ameliorate the functional damage that occurs. | 1997 | 9381535 |
| protection of canine pancreatic microvascular endothelium against cold ischemic injury during preservation by the two-layer method. | ischemic injury of the microvascular endothelium during cold preservation causes a disturbance of vascular microcirculation after reperfusion and results in graft failure. recently we have shown that oxygenation of the canine pancreas during preservation by the two-layer method extends the period of preserved pancreatic viability. the aim of this study was to clarify the role of the oxygenation of the pancreas graft by the two-layer method in the viability of the microvascular endothelium during ... | 1997 | 9381539 |
| [ouabain-containing euro-collins solution prevents atn following renal cold storage]. | in view of the hypothesis that suppression of energy demand may prevent ischemic cell damage, it seemed possible that suppression of atp utilization during ischemia might ameliorate the severity of renal failure following kidney preservation. to test this possibility, a short-term in situ kidney preservation model was prepared in dogs. euro-collins solution containing 10(-5) m ouabain (o-ec) was used as the preservation solution. the kidney was preserved with cold o-ec for two hours and reperfus ... | 1997 | 9396237 |
| in vitro amylase release of preserved pancreas: a simple test to assess the viability of pancreatic allograft during preservation in the pigs. | to determine an in vitro marker of viability during pancreatic preservation, 12 pigs underwent total pancreas harvesting, and graft were stored in euro-collins or belzer perfusion solution for up to 24 hours. amylase concentration of the storage solution was analyzed in regular periods and tissue samples were taken for acridine-orange histochemical evaluation of viability in the same time. in vitro pancreatic amylase release (iu/g pancreas tissue) was calculated from the volume of solution and t ... | 1997 | 9408282 |
| experimental investigation of preservation injury in animal kidneys after reperfusion with euro-collins. | the authors evaluated the pathomorphologic alterations of removed and reperfused dog kidneys by means of light and electronmicroscopic examination. in each sample the following reversible signs were found: hypereosinophilia (he), hydropic dystrophy (hd), nuclear polymorphism (np), epithelial desquamation (ed), brush border lesion (bbl), single cell necrosis (scn), total tubular epithel necrosis (tten), interstitial edema (ie), perivascular edema (pe). the irreversible signs were: basement membra ... | 1997 | 9408343 |
| protection of the renal artery in nephron-sparing surgery. i. pathomorphological study. | it is necessary to clamp the renal artery for some time in renal surgery to preserve the organ. not only the renal parenchyma but also the wall of the clamped renal artery may be damaged due to known ischaemic-reperfusion causes. regional venous renal hypothermia induced by intracellular solutions (sacks ii, euro-collins) could provide protection against damage of the vessel wall caused by reperfusion or clamping in our experiments, in cases of clamping for 30 minutes, probably because it also e ... | 1997 | 9408357 |
| efficacy of prostaglandin i2 analog on liver grafts subjected to 30 minutes of warm ischemia. | due to the fact that no effective conditioning agent for hemodynamically unstable donors exists, the number of suitable donors is limited. the efficacy of op-41483, a stable analog of prostaglandin i2, as a conditioning agent of the liver was investigated in this study using pigs. op-41483 was administered via the portal tributary for 10 min before 30 min of warm ischemia. graft livers were procured after perfusion with op-41483 in cold normal saline, then flushed with op-41483 in euro-collins s ... | 1997 | 9413060 |
| effect of pancreatic warm ischemia on islet yield and viability in dogs. | defining tolerable warm ischemia (wi) is mandatory before nonheartbeating cadavers can be used to enlarge the donor pool. no studies to date have precisely evaluated the effect of pancreatic wi on islet yield and viability in a large animal model. | 1997 | 9422394 |
| increase of c-reactive protein and decrease of surfactant protein a in surfactant after lung transplantation. | in this study, we asked whether the serum acute-phase protein c-reactive protein (crp) increased in large surfactant aggregates after lung transplantation and analyzed the changes in composition and interfacial adsorption activity of those aggregates. single left lung transplantation was performed in weight-matched pairs of dogs. a double-lung block from the donor animal was flushed with either modified euro-collins solution (ec) (n = 6) or university of wisconsin solution (uw) (n = 6) at 4 degr ... | 1998 | 9445277 |
| comparative effects of university of wisconsin and euro-collins solutions on pulmonary mitochondrial function after ischemia and reperfusion. | the aim of this study was to compare the effects of euro-collins and university of wisconsin solutions on pulmonary mitochondrial function after cold ischemia and subsequent warm reperfusion. | 1998 | 9458008 |
| carbohydrate selectin inhibitor cy-1503 reduces neutrophil migration and reperfusion injury in canine pulmonary allografts. | neutrophil adhesion is initiated by the interaction of rapidly expressed endothelial selectins with oligosaccharide structures (sialyl lewis(x) on polymorphonuclear neutrophils (pmn). the carbohydrate sialyl lewis x analogue cy-1503 blocks selectin receptors, thereby inhibiting pmn rolling and subsequent firm adhesion and migration. | 1997 | 9361248 |
| preharvest nitroprusside flush improves posttransplantation lung function. | morbidity as a result of early allograft dysfunction remains a significant problem in clinical lung transplantation. we previously demonstrated that nitroprusside (np), a potent nitric oxide donor, administered before storage and again during reperfusion, reduced lung reperfusion injury. the purpose of the present study was to determine whether these observations were storage effects, reperfusion effects, or both. | 1997 | 9361250 |
| in vivo measurement of lung preservation solution efficacy: comparison of lpd, uw, ec and low k+-ec following short and extended ischemia. | the impact of storage solution composition on graft performance was evaluated following perfusion with either euro-collins (ec), low potassium euro-collins (rec), low potassium dextran (lpd) or university of wisconsin solution (uw) after brief (2 h) and extended ischemia (16 h) in an acute double lung transplantation model in the rat. | 1997 | 9458150 |
| ultrastructural pathology of the alveolar type ii pneumocytes of human donor lungs. electron microscopy, stereology, and microanalysis. | alveolar type ii pneumocytes (pii) were studied in 12 human donor lungs perfused with modified euro-collins solution during single-lung transplantation (sltx). while one lung was transplanted, the contralateral donor lung (cdl) was fixed at the time of sltx for examination by electron microscopy, stereology, and microanalysis. three groups were then formed: group a (n = 7), cdl without contusions, uneventful early postoperative course; group b (n = 3), cdl with conclusions, uneventful early post ... | 1998 | 9532002 |
| addition of a mast cell stabilizing compound to organ preservation solutions decreases lung reperfusion injury. | research in lung transplant preservation has generally focused on free radicals and enzyme release from neutrophils, parenchymal cells, macrophages, and endothelium. the lung has a large resident population of mast cells that, when activated, release potent inflammatory mediators. we hypothesized that adding an inhibitor of mast cell degranulation, lodoxamide tromethamine (10 micromol/l), to euro-collins and university of wisconsin preservation solutions, would decrease lung preservation injury. | 1998 | 9535451 |
| inductively coupled plasma mass spectrometric determination of molybdenum in urine from a danish population. | molybdenum creatinine levels in urine were measured in 128 danish inhabitants by icp-ms in order to establish reference intervals of molybdenum in urine for the danish population as a part of the euro-terviht project (trace element reference values in human tissues). the mo concentration was determined using the isotopes 95mo and 98mo. the values measured based on 98mo were about 2% lower than those calculated using 95mo, a negligible difference in the context of reference values. the limit of d ... | 1998 | 9581025 |
| interruption of bronchial circulation leads to a severe decrease in peribronchial oxygen tension in standard lung transplantation technique. | in clinical practice lung transplantation is the only procedure where the transplanted organ is left without its own arterial perfusion. with the interruption of the bronchial arteries the nutritive support is dependent on collateral flow by the pulmonary artery and the oxygen tension of desaturated central venous blood, representing an abnormal physiology. | 1998 | 9583824 |
| apoptosis of sinusoidal endothelial cells is a critical mechanism of preservation injury in rat liver transplantation. | in livers excised for transplantation, sinusoidal endothelium appears especially vulnerable to injury during organ preservation in the cold and subsequent reperfusion. the degree of endothelial cell injury correlates with functional impairment of the graft following transplantation. the mechanism of injury remains obscure, but endothelial cell damage has been described as coagulative necrosis secondary to irreversible physico-chemical damage. we investigated whether endothelial cell death is cau ... | 1998 | 9620339 |
| the effects of thyroid hormone modulation on rat liver injury associated with ischemia-reperfusion and cold storage. | we investigated the effects of thyroid hormone modulation on liver injury associated with ischemia-reperfusion (i-r) and cold storage in rats. first, euthyroid and thyroxine (t4)-pretreated rats were exposed in vivo to 20-min global liver ischemia, then 30-min reperfusion. liver injury was assessed by measuring serum alanine aminotransferase (alt) levels. liver concentrations of adenine nucleotides, reduced glutathione (gsh), and oxidized glutathione were evaluated. second, rats were given the a ... | 1998 | 9620501 |
| controlled reperfusion and pentoxifylline modulate reperfusion injury after single lung transplantation. | rodent models have suggested that initial low-pressure reperfusion of transplanted lungs reduces injury after ischemia. we investigated this phenomenon and the use of pentoxifylline in a porcine model of left single lung transplantation. | 1998 | 9628676 |
| [isolation of the pig islets of langerhans: evaluation of in vitro and in vivo function]. | pig islets are considered the best alternative to human islets in the treatment of insulin-dependent diabetes. pigs could represent a potential islet donor for xenotransplantation in humans because of the close similarity between human and porcine insulin and the theoretically unlimited availability of porcine pancreas. from november 1991 to january 1997 we performed 221 pig islet isolations from 3 pig sources: group 1: minipigs (age 9-18 months) and white pigs (3-8 months), group 2: large white ... | 1998 | 9653822 |
| smoking prevalence and risk factors for smoking in a population of united states air force basic trainees. | to provide a comprehensive assessment of smoking prevalence and risks for smoking in an entire population of united states air force (usaf) military basic trainees (n = 32,144). | 1998 | 9825416 |
| comparative study of solutions for pulmonary preservation using an isolated rabbit lung model. | at the university of minnesota, university of wisconsin (uw), modified euro-collins (mec), and marshall (m) solutions were compared as agents for pulmonary preservation in an isolated rabbit lung model. normal saline (ns) was used as a control. the heart-lung blocks of donor rabbits were flushed with, and then preserved in, one of the solutions at 4 degrees c. five rabbits were studied in each group. after 8 h of cold ischemia, the left lung was ventilated and reperfused with fresh venous blood ... | 1998 | 9655094 |
| evaluation of new improved solution containing trehalose in free skin flap storage. | in this study, we evaluated a new intracellular type (it-k) solution containing trehalose in a rabbit free skin flap storage model. trehalose is a nonreducing disaccharide that can stabilise cell membranes under various stressful conditions. | 1998 | 9659114 |
| organ preservation with ec, htk, and uw solutions in orthotopic liver transplantation in syngeneic rats. part i: functional parameters. | ischemic injury to the liver is known to influence the outcome of liver transplantation. in this study the efficacy of euro-collins (ec), histidine-tryptophan-ketoglutarate (htk), and university of wisconsin (uw) preservation solution was analyzed in the model of orthotopic liver transplantation in syngeneic rats. the study design was as follows: group i, euro-collins solution (n = 11); group ii, histidine-tryptophan-ketoglutarate solution (n = 11); group iii, university of wisconsin solution (n ... | 1998 | 9659636 |
| lipid peroxidation after cold storage and normothermic reperfusion: the effect of trimetazidine. | ischemia reperfusion injury is still a leading cause of early graft dysfunction after transplantation. trimetazidine (tmz) has been postulated to be protective against renal damage from oxygen free radicals. the aim of this study was to assess the effect tmz during cold storage (cs) and normothermic reperfusion in an isolated perfused pig kidney model. three groups were studied: control group, immediately perfused (g0), 48 h cs in euro-collins solution (g1), and 48 h cs in euro-collins solution ... | 1998 | 9665028 |
| kupffer cell activation in the survival discrepancy between liver grafts from enterally and parenterally fed donors. | this study was designed to investigate the effects of differences in the route of nutritional support of the donor on cold ischemia/reperfusion injury. participation of kupffer cells in these effects, based on the analysis of hepatic energy metabolism in early phases of reperfusion was also investigated. orthotopic liver transplantation was performed between large-white pigs weighing 20-30 kg after a 4-h cold preservation of the graft in euro-collins solution at 4 degrees c. one group was fed or ... | 1998 | 9665029 |
| addition of aprotinin to organ preservation solutions decreases lung reperfusion injury. | organ preservation injury is associated with endothelial cell damage, destabilization of mitochondrial and cell membranes, and the release of proteolytic enzymes. in addition to its well-known clinical effect of reducing perioperative blood loss, aprotinin has antiproteolytic and membrane-stabilizing properties. we hypothesized that adding aprotinin to euro-collins (ec) and university of wisconsin (uw) solutions would decrease preservation injury in cultured endothelial cells and a whole organ r ... | 1998 | 9692469 |
| protective effect of ischemic preconditioning on liver preservation-reperfusion injury in rats. | ischemic-preconditioning is a process whereby a brief ischemic episode confers a state of protection against subsequent long-term ischemia-reperfusion injury. ischemic preconditioning has been studied in heart and liver ischemia-reperfusion injury; however, few studies have been performed in the model of preservation-reperfusion injury in liver transplantation. the current study was designed to evaluate the ability of ischemic preconditioning to protect liver grafts from long-term preservation-r ... | 1998 | 9701256 |
| influence of the potassium concentration on functional and structural preservation of the lung: where is the optimum? | low-potassium solutions have been shown to improve lung preservation. the optimal potassium concentration, however, has not been investigated systematically. the purpose of this study was to evaluate the effect of solutions with different potassium concentrations on functional and structural preservation after flush-perfusion and ischemia. we used our established extracorporeal working heart-lung model and a modification of this model with isolated pulmonary perfusion at defined flow rates. | 1998 | 9703238 |
| evaluation of 18-hour lung preservation with oxygenated blood for optimal oxygen delivery. | previous studies have shown that availability of oxygen during lung preservation to maintain aerobic metabolism may be essential for the optimal viability of preserved lung tissue. the purpose of this study was to evaluate lung preservation with oxygenated blood for optimal oxygen delivery to the lung graft in a rabbit model. | 1998 | 9725370 |
| [morphofunctional state of ischemically damaged kidney transplant in preservation and reperfusion (an experimental study)]. | in cadaveric renal transplantation with simple cold storage, pretransplant evaluation of allograft viability creates an important problem. to assess the degree of ischemic damage and microcirculation of the renal allograft, experiment comparing parameters, like median perfusate flow and median intrarenal resistance, with histological findings was made. kidney harvesting was performed from three groups of new zealand rabbits by keeping warm ischemic time zero, thirty, sixty minutes for each group ... | 1998 | 9727318 |
| comparison of euro-collins solution, low-potassium dextran solution containing glucose, and et-kyoto solution for lung preservation in an extracorporeal rat lung perfusion model. | | 1998 | 9731097 |
| women's health issues cause controversy in european union. | women's health issues will be raised in the european parliament in september, when members return from vacation. nel van dijk, dutch green party deputy, produced a report to the plenary for the assembly's committee on women's rights; in the report, committee members urge action by the european union (eu) and list recommendations for member-state governments. the report is in response to a publication by the european commission, in may 1997, of a statistical survey developed from national data. ... | 1998 | 9746038 |
| the effect of trehalose on human lung fibroblasts stored in euro-collins and low potassium dextran solutions. | the research on lung preservation for transplantation is directed to synthesize a solution, in order to prolong the preservation period, thus avoiding the damage to lung tissues caused by ischemia. in recent years some in vivo experimental papers have been published, in which good results were obtained by adding trehalose, a non-reducing disaccharide able to stabilize the cell membrane, to the storage solutions. | 1997 | 9461277 |
| cold preservation of isolated rabbit proximal tubules induces radical-mediated cell injury. | reactive oxygen species (ros) are involved in reperfusion injury after preservation. recent studies in isolated endothelial cells and hepatocytes suggested the occurrence of ros-mediated injury during the period of cold incubation. in the present study, formation of ros and subsequent cell injury were studied in freshly isolated rabbit proximal tubules (pts). | 1998 | 9521195 |
| an isocratic, reversed-phase hplc method for the determination of postischemic efflux of purines and pyrimidines during reperfusion of isolated liver. | an isocratic, reversed-phase hplc method was developed for the determination of the rates of purine and pyrimidine efflux during early reperfusion of isolated organs after non-perfusion cold conservation. the method enables determination of uric acid, cytidine, xanthine, hypoxanthine, uridine, amp, inosine, and adenosine in liver perfusate using a standard c-18 column (25 cm length). peaks are resolved by elution with buffer containing 1% acetonitrile, 20 mm potassium citrate (ph 6.25), and 25-5 ... | 1998 | 9526118 |
| the changes in main pulmonary artery pressure after bilateral lung autotransplantation in dogs. | in the respiratory center, yongdong severance hospital, yonsei university, we performed 10 cases of bilateral lung autotransplantation in mongrel dogs from july 1994 to june 1996, and we have analyzed the hemodynamic changes. autotransplantation was performed in order to avoid postoperative rejection. the lung was flushed with an euro-collins(e-c) solution containing pge1 which passed through a 10 french catheter inserted into an incision on the anterior half of the pulmonary artery to pulmonary ... | 1998 | 9752794 |
| controlled reperfusion prevents pulmonary injury after 24 hours of lung preservation. | posttransplantation lung reperfusion injury continues to be a major problem. we have shown that controlling the initial period of reperfusion limits this injury after 2 hours of warm lung ischemia. the effectiveness of this modality, however, is unknown after longer periods of cold ischemia, which more closely mimics the clinical situation. | 1998 | 9768945 |
| protective effects of ischemic preconditioning on donor lung in canine lung transplantation. | myocardial ischemic preconditioning has been found to protect the myocardium. we hypothesized that lung ischemic preconditioning might enhance canine lung preservation and reduce allograft lung dysfunction after transplantation. | 1998 | 9596319 |
| function of rat skeletal muscles after storage at 10 degrees c in various preservation solutions. | 1. the purpose of this study was to assess the potential of various preservation solutions, orginally designed for solid organs, to protect muscle function during cold storage. 2. the soleus (sol) and the cutaneous trunci (ct) muscle from the rat were isolated and stored for 2, 4 or 8 h at 10 degrees c. the solutions used, listed in order from an intracellular to an extracellular-like composition, were: university of wisconsin (uw), euro-collins (ec), htk-bretschneider (htk), reversed st. thomas ... | 1998 | 9616261 |
| selected polyhalogenated hydrocarbons in breast milk. | breast milk samples were collected and analysed within a comprehensive programme co-ordinated by who euro to evaluate the possible health risk for breast-fed infants in chosen localities of european countries ('exposed' and 'control' ones). the samples of breast milk were collected, stored transported and analysed by a standardised study protocol to assure the comparability of the results from different areas (of 19 european countries). the study included three categories of chemicals: polychlor ... | 1998 | 9820686 |
| lap-1 cold preservation solution for isolation of high-quality human pancreatic islets. | the most critical factors that affect the outcome of clinical pancreatic islet transplantation are the number and quality of donor islets available for transplantation. toward this goal, we attempted to obtain islets that are both of better quality and higher number than are obtainable by the islet-isolation process that is now widely used. we paid special attention to two critical components of the isolation procedure: minimizing the exposure of pancreatic tissue and freed islets to warm enzyme ... | 1998 | 9821178 |
| relationships between cytochrome oxidase (co) blobs in primate primary visual cortex (v1) and the distribution of neurons projecting to the middle temporal area (mt). | the cytochrome oxidase (co) blobs and interblobs in layer 3b of primate visual cortex have different sets of corticocortical connections. cortical layers below layer 3b also project corticocortically, but the relationship of efferent projections from the deeper layers to the overlying blob/interblob architecture is less clear. we studied the tangential organization of neurons projecting from primary visual cortex (v1) to the middle temporal visual area (mt) and their relationship to the co blobs ... | 1999 | 10376741 |
| coronary oxygen persufflation for long-term myocardial protection. | in human heart transplantation limited myocardial ischemia duration remains one of the most restricting factors. a new approach towards prolongation of this duration is the combination of cardioplegic arrest and continuous coronary oxygen persufflation (cop) with gaseous oxygen. this technique, which is based on former experiments, was applied in pig hearts which we transplanted orthotopically after a hypothermic preservation time of 14 hours. for cardioplegic arrest we used either euro-flush gl ... | 1998 | 9822185 |
| a stable prostacyclin analog, beraprost sodium, attenuates platelet accumulation and preservation-reperfusion injury of isografts in a rat model of lung transplantation. | recent studies have shown that the extent of platelet accumulation in the vasculature of transplanted organs correlates with the degree of preservation-reperfusion injury. in this study, we examined the effect of a stable prostacyclin analog, beraprost sodium, which possesses potent antiplatelet activity, on parameters of platelet accumulation and preservation-reperfusion injury of isografts in a rat model of lung transplantation. | 1998 | 9825806 |
| report of the who working group on the assessment of health risks for human infants from exposure to pcdds, pcdfs and pcbs. | on nov. 20-22, 1995, a world health organization working group consisting of 12 scientific representatives from 6 different countries met to reassess the health risks to infants associated with perinatal exposure to polyhalogenated aromatic hydrocarbons (phahs). following a review of previous who/euro consultations, as part of their comprehensive programme on pcdds, pcdfs and pcbs, current exposure information and recent experimental and epidemiologic data were discussed. exposure assessments wi ... | 1998 | 9828293 |
| [effect of ischemic preconditioning on adenine nucleotide levels of graft lung from canine donor]. | twelve canine left lung allotransplantation were performed. in the ischemic preconditioning group (group ip, n = 6), left donor lung was preconditioned with 10 min ischemia followed by 15 min reperfusion using the occuluding left hilum before resection and cold perfusion. the control group (group c, n = 6) underwent the same treatment but without ischemic preconditioning. adenine nucleotides (atp, adp, amp) of the donor lung tissue were measured using rhplc after 2 hr of resection and cold perfu ... | 1997 | 9868017 |
| [effects of ischemic preconditioning on protection of canine donor lung]. | the authors examined the effects of ischemic preconditioning on lung preservation using a canine single left lung transplantation. twelve adult mongrel dogs underwent left lung allotransplantation. donor lungs were perfused and flushed with cold euro-collins solution (ecs) and stored at 4 degrees c ecs for 2 hours. six donors were preconditioned by occuluding left lung hilum for 10 minutes and releasing for 15 minutes before flushing (group pc); other six donors without ischemic preconditioning ... | 1997 | 9868110 |
| excellent recovery after prolonged heart storage by preservation with coronary oxygen persufflation: orthotopic pig heart transplantations after 14-hr storage. | improvement of heart preservation is still the greatest challenge in preservation research. the unchanged severe restriction of acceptable storage periods of heart grafts since the beginning of clinical heart transplantation indicates that technical innovations are necessary if a substantial improvement is to be achieved. | 1998 | 9869085 |
| de novo cancers complicating renal transplantation: experience in the nordic countries. | this report summarizes the experience in the nordic countries with de novo cancer complicating renal transplantation and the prospects of a new nordic eu-sponsored study (euro-cat) covering all kinds of organ transplantations. | 1997 | 9869875 |
| preservation with 8-bromo-cyclic gmp improves pulmonary function after prolonged ischemia. | cyclic guanosine monophosphate (cgmp) is a potent second messenger for the nitric oxide pathway in the pulmonary vasculature. increased cytosolic cgmp levels elicit pulmonary vasodilatation resulting in decreased pulmonary vascular resistance and maximized pulmonary function after ischemia-reperfusion injury. we hypothesized that the addition of a membrane-permeable cgmp analogue (8-bromo-cgmp) to a euro-collins (ec) preservation solution would ameliorate pulmonary reperfusion injury better than ... | 1998 | 9875780 |
| a comparison of the new preservation solution celsior to euro-collins and university of wisconsin solutions in lung reperfusion injury. | the lung is particularly susceptible to reperfusion injury, both experimentally and clinically after transplantation. the extracellular-type preservation solution celsior, which has been predominantly studied in cardiac preservation, has components designed to prevent cell swelling, free radical injury, energy depletion, and calcium overload. using an isolated blood-perfused rat lung model, we investigated whether celsior would decrease preservation injury and improve lung function after cold is ... | 1999 | 9921812 |
| transalveolar fluid absorption ability in rat lungs preserved with euro-collins solution and ep4 solution. | pulmonary reimplantation response, presenting lung edema, is a major obstacle of lung transplantation. transalveolar fluid absorption mechanism, regulated by active transalveolar na+ transport via na+ channel and na+-k+-atpase, is considered to be essential for resolution of lung edema. we investigated the effect of lung preservation on this fluid transport mechanism. | 1999 | 10030277 |
| modulation of reperfusion injury after single lung transplantation by pentoxifylline, inositol polyanions, and sin-1. | previous studies have suggested reductions in lung reperfusion injury with pentoxifylline, inositol polyanions, and the nitric oxide donor, sin-1, but these agents have never been directly compared to ascertain which is superior. we investigated these agents in a porcine model of left single lung transplantation. | 1999 | 10047660 |
| histological evaluation of proximal tubule cell injury in isolated perfused pig kidneys exposed to cold ischemia. | ischemic injury of the renal allograft before transplantation is a major cause of impaired graft function. proton nuclear spectroscopy provides a useful technique for evaluating proximal tubular activity. in addition to this technique, we proposed a histological grading system for quantifying proximal tubule alterations. | 1999 | 10090834 |
| improvement of rat lung structure and function after preservation with celsior. | ischemia/reperfusion-induced increase in pulmonary microvascular permeability was shown to be reduced after preservation with celsior. we investigated reimplantation-induced lung injury in isolated, reperfused rat lungs after preservation via the pulmonary artery with celsior, celsior + prostacyclin, and reduced-potassium (40 mmol) euro-collins solution (40 ml/kg/body wt each) followed by 2 h of cold ischemia. arterial and veneous oxygen tensions were recorded during 50 min of in vitro reperfusi ... | 1999 | 10090841 |
| does pge1 attenuate potassium-induced vasoconstriction in initial pulmonary artery flush on lung preservation? | the standard clinical protocol for lung transplantation employs cold single pulmonary artery flush with euro-collins solution or the university of wisconsin solution. prostaglandin e1 (pge1) is usually given by direct injection into the pulmonary artery to reduce pulmonary vasoconstriction caused by these intracellular, high-potassium solutions, however, the efficacy of pge1 on lung preservation remains controversial. in this study we demonstrated that vasodilator effects of pge1 were markedly r ... | 1999 | 10194037 |
| effect of coenzyme q10 on superoxide production in rats with reperfusion injuries. | we examined the effect of coenzyme q10 (co q10) on superoxide radical (o2-) production in a model of rat reperfusion injury. the chemiluminescence method using a derivative of luciferin was used to quantify o2- production by erythrocytes in the reperfused limb after a period of ischaemia. a total of 20 limbs from lewis rats were preserved at 4 degrees c in euro-collins solution for 72 hours, and were grafted orthotopically to syngeneic rats by a microsurgical technique. in the treated group (n = ... | 1999 | 10207958 |
| mitochondrial injuries in rat lungs preserved for 17 h: an ultrastructural study. | mitochondria of the small vasculature endothelial cells were examined in preserved rat lungs before and after reperfusion, and the ultrastructural changes were correlated with pulmonary function after reperfusion. rat lungs were flushed with perfusate and prostaglandin e1 and divided into five groups (n = 5 in each group): group a, normal control group; group b, university of wisconsin solution; group c, euro-collins solution; group d, et-kyoto solution, and group e, new et-kyoto solution. after ... | 1999 | 10213855 |
| stereological estimation of the volume weighted mean volumes of alveoli and acinar pathways in the rat lung to characterise alterations after ischaemia/reperfusion. | the aim of this study was to characterise pulmonary reimplantation injury in isolated, perfused rat lungs following 2 h of cold ischaemia, and 50 min. of in vitro reperfusion. the effects of 2 differently composed lung preservation solutions (low potassium euro-collins and celsior; each n = 5) were examined in comparison with untreated, nonischaemic control lungs (n = 3). after fixation by vascular perfusion and tissue collection by systematic random sampling, the volume weighted mean volume (vv ... | 1999 | 10227674 |
| pulmonary ischemia/reperfusion injury: a quantitative study of structure and function in isolated heart-lungs of the rat. | early graft dysfunction after lung transplantation is a significant and unpredictable problem. our study aimed at a detailed investigation of structure-function correlations in a rat isolated heart-lung model ofischemia/ reperfusion injury. variable degrees of injury were induced by preservation with potassium-modified euro-collins solutions, 2 hr of cold ischemia, and 40 min of reperfusion. pulmonary artery pressure (ppa), pulmonary vascular resistance (pvr), peak inspiratory pressure (pip), an ... | 1999 | 10321996 |