| a continuously monitored colorimetric method for detection of mycobacterium tuberculosis complex in sputum. | mbeya, tanzania. | 2013 | 24200277 |
| vitamin b(12) metabolism in mycobacterium tuberculosis. | mycobacterium tuberculosis is included among a select group of bacteria possessing the capacity for de novo biosynthesis of vitamin b12, the largest and most complex natural organometallic cofactor. the bacillus is also able to scavenge b12 and related corrinoids utilizing an atp-binding cassette-type protein that is distinct from the only known bacterial b12-specific transporter, btufcd. consistent with the inferred requirement for vitamin b12 for metabolic function, the m. tuberculosis genome ... | 2013 | 24199800 |
| mbth homology codes to identify gifted microbes for genome mining. | advances in dna sequencing technologies have made it possible to sequence large numbers of microbial genomes rapidly and inexpensively. in recent years, genome sequencing initiatives have demonstrated that actinomycetes with large genomes generally have the genetic potential to produce many secondary metabolites, most of which remain cryptic. since the numbers of new and novel pathways vary considerably among actinomycetes, and the correct assembly of secondary metabolite pathways containing typ ... | 2014 | 24197465 |
| plakinamine m, a steroidal alkaloid from the marine sponge corticium sp. | by means of bioassay-guided fractionation, a new steroidal alkaloid, plakinamine m (1), and the known compound, plakinamine l (2), with a unique acyclic side chain, were isolated from the marine sponge corticium sp. collected from new britain, papua new guinea. the structures were determined on the basis of extensive 1d and 2d nmr and hresims. the two compounds showed inhibition of mycobacterium tuberculosis with mic values of 15.8 and 3.6 μg/ml, respectively. | 2013 | 24195491 |
| cholera toxin enhances vaccine-induced protection against mycobacterium tuberculosis challenge in mice. | interleukin (il)-17 is emerging as an important cytokine in vaccine-induced protection against tuberculosis disease in animal models. here we show that compared to parenteral delivery, bcg delivered mucosally enhances cytokine production, including interferon gamma and il-17, in the lungs. furthermore, we find that cholera toxin, delivered mucosally along with bcg, further enhances il-17 production by cd4(+) t cells over mucosal bcg alone both in the lungs and systemically. this boosting effect ... | 2013 | 24194918 |
| mechanism of inhibition of mycobacterium tuberculosis antigen 85 by ebselen. | the increasing prevalence of drug-resistant tuberculosis highlights the need for identifying new antitubercular drugs that can treat these infections. the antigen 85 (ag85) complex has emerged as an intriguing mycobacterial drug target due to its central role in synthesizing major components of the inner and outer leaflets of the mycobacterial outer membrane. here we identify ebselen (ebs) as a potent inhibitor of the mycobacterium tuberculosis ag85 complex. mass spectrometry data show that ebs ... | 2013 | 24193546 |
| comparison of bcg prime-dna booster and rbcg regimens for protection against tuberculosis. | developing an effective adult prophylaxis vaccine is a high priority in the global control of tuberculosis (tb), because tb remains an important public health problem and the current widely used bcg vaccine provides effective protection only for children but variable protection against adult tb. bcg priming-heterologous vaccines booster and recombinant bcg technologies have been thought as two important regimens for inducing effective protection against adult tb. obviously, defining the protecti ... | 2014 | 24192709 |
| multicentric analysis of childhood tuberculosis in turkey. | only a few series of pediatric tuberculosis (tb) have been reported in the last 20 years. the purpose of this study was to evaluate the clinical, radiological, microbiological, and treatment characteristics of childhood tb. a total of 539 children with childhood tb diagnosed over a 12-year period (1994-2005) in 16 different centers in turkey participated in the study. the medical records of all childhood tb patients were investigated. a total of 539 children (274 males, 265 females) with childho ... | 2013 | 24192671 |
| catalytic synthesis of enantiopure mixed diacylglycerols – synthesis of a major m. tuberculosis phospholipid and platelet activating factor. | an efficient catalytic one-pot synthesis of tbdms-protected diacylglycerols has been developed, starting from enantiopure glycidol. subsequent migration-free deprotection leads to stereo- and regiochemically pure diacylglycerols. this novel strategy has been applied to the synthesis of a major mycobacterium tuberculosis phospholipid, its desmethyl analogue, and platelet activating factor. | 2013 | 24191360 |
| a genetic strategy to identify targets for the development of drugs that prevent bacterial persistence. | antibacterial drug development suffers from a paucity of targets whose inhibition kills replicating and nonreplicating bacteria. the latter include phenotypically dormant cells, known as persisters, which are tolerant to many antibiotics and often contribute to failure in the treatment of chronic infections. this is nowhere more apparent than in tuberculosis caused by mycobacterium tuberculosis, a pathogen that tolerates many antibiotics once it ceases to replicate. we developed a strategy to id ... | 2013 | 24191058 |
| inhibition of toll-like receptor 2 (tlr-2)-mediated response in human alveolar epithelial cells by mycolic acids and mycobacterium tuberculosis mce1 operon mutant. | in human lungs, the earliest encounter of mycobacterium tuberculosis, the agent of tuberculosis, involves alveolar epithelial cells. droplets expectorated by a patient with tuberculosis are likely to contain a mixed population of m. tuberculosis cells in different physiologic and metabolic states from the lung lesions of the patient. here, we compared the chemokine expression patterns of human epithelial cell line a549 and raw 264.7 macrophage cells infected with wild-type m. tuberculosis h37rv ... | 2014 | 24190334 |
| high prevelance of rifampin-monoresistant tuberculosis: a retrospective analysis among iranian pulmonary tuberculosis patients. | we determined the prevalence of rifampin-monoresistant tuberculosis (rmr-tb) in iran. because development of rmr-tb is not common, we also identified the major risk factors associated with rmr-tb reported from different provinces of iran. data for 3,020 tb patients who remained or became smear positive after two, four, six, and nine months of standard first-line chemotherapy were retrospectively analyzed. of 3,020 patients, 1,242 patients (41.1%) were culture and dna positive for mycobacterium t ... | 2014 | 24189362 |
| differences in gene expression between clonal variants of mycobacterium tuberculosis emerging as a result of microevolution. | clonal variants of mycobacterium tuberculosis can emerge as a result of microevolution in a single host or after sequential infection of different hosts. the significance of subtle genotypic variations is still unknown. in three of the four loci analyzed from clonal variants differing in only one miru-vntr locus, we found that the expression of the adjacent genes was modulated differently. these data highlight the potential advantages that acquisition of subtle variability may have in m. tubercu ... | 2013 | 24189285 |
| moxifloxacin population pharmacokinetics and model-based comparison of efficacy between moxifloxacin and ofloxacin in african patients. | pharmacokinetic exposure and the mic of fluoroquinolones are important determinants of their efficacy against mycobacterium tuberculosis. population modeling was used to describe the steady-state plasma pharmacokinetics of moxifloxacin in 241 tuberculosis (tb) patients in southern africa. monte carlo simulations were applied to obtain the area under the unbound concentration-time curve from 0 to 24 h (fauc0-24) after daily doses of 400 mg or 800 mg moxifloxacin and 800 mg ofloxacin. the mic dist ... | 2014 | 24189253 |
| manganese(ii) complexes with thiosemicarbazones as potential anti-mycobacterium tuberculosis agents. | through a systematic variation on the structure of a series of manganese complexes derived from 2-acetylpyridine-n(4)-r-thiosemicarbazones (hatc-r), structural features have been investigated with the aim of obtaining complexes with potent anti-mycobacterium tuberculosis activity. the analytical methods used for characterization included ftir, epr, uv-visible, elemental analysis, cyclic voltammetry, magnetic susceptibility measurement and single crystal x-ray diffractometry. density functional t ... | 2014 | 24188534 |
| clonal expansion of mycobacterium tuberculosis isolates and coexisting drug resistance in patients newly diagnosed with pulmonary tuberculosis in hanoi, vietnam. | newly diagnosed patients without anti-tuberculosis (tb) treatment histories have not often undergone drug susceptibility testing (dst), but have received the standard treatment regimen without information about their dst profiles in many countries with inadequate resources. | 2013 | 24188178 |
| early stationary phase culture supernatant accelerates growth of sputum cultures collected after initiation of anti-tuberculosis treatment. | we investigated the effect of mycobacterium tuberculosis culture supernatant added to sputum cultures collected during the first 8 weeks of anti-tuberculosis treatment. with ongoing treatment duration, time to culture positivity decreased significantly in supernatant-enriched cultures, possibly due to stimulation of dormant or slowly metabolizing m. tuberculosis cells. | 2014 | 24188165 |
| mycobacteria and disease in southern africa. | the genus mycobacterium consists of over 120 known species, some of which (e.g. m. bovis and m. tuberculosis) contribute extensively to the burden of infectious disease in humans and animals, whilst others are commonly found in the environment but may rarely if ever be disease-causing. this paper reviews the mycobacteria found in southern africa, focussing on those in the m. tuberculosis complex as well as the non-tuberculous mycobacteria (ntm), identifying those found in the area and including ... | 2013 | 24171860 |
| temporal trend of tuberculosis in wild ungulates from mediterranean spain. | tuberculosis (tb), a chronic disease caused by infection with the mycobacterium tuberculosis complex, is endemic in wild boar (sus scrofa) and red deer (cervus elaphus) in south-central spain. understanding the temporal dynamics of this chronic infection requires long time series data collection over large areas. the aim of this paper was to identify the determinants of tb prevalence and severity in both species in ciudad real province, spain, from 2000 to 2012. study variables included manageme ... | 2013 | 24171854 |
| the elephant interferon gamma assay: a contribution to diagnosis of tuberculosis in elephants. | mycobacterium tuberculosis (m. tb) has been shown to be the main causative agent of tuberculosis in elephants worldwide. m. tb may be transmitted from infected humans to other species including elephants and vice versa, in case of prolonged intensive contact. an accurate diagnostic approach covering all phases of the infection in elephants is required. as m. tb is an intracellular pathogen and cell-mediated immune (cmi) responses are elicited early after infection, the skin test is the cmi assay ... | 2013 | 24171849 |
| mycobacterium tuberculosis at the human/wildlife interface in a high tb burden country. | this study reports on an investigation of mycobacterium tuberculosis cases in mostly captive wild animals using molecular typing tools [variable number of tandem repeat (vntr) typing and restriction fragment length polymorphism typing]. the investigation included cases from (i) the national zoological gardens of south africa (nzg) recorded between 2002 and 2011; (ii) johannesburg zoo, where tuberculosis was first diagnosed in 2007 and has since been detected in three antelope species; (iii) a re ... | 2013 | 24171848 |
| review of the diagnosis of tuberculosis in non-bovid wildlife species using immunological methods--an update of published work since 2009. | the infection of both captive and free-ranging wildlife species with pathogenic mycobacteria (including mycobacterium tuberculosis) poses a zoonotic risk and continues to cause challenges for the livestock industry, zoos and governments around the world. central to the management and control of tuberculosis is timely and accurate diagnosis. in many cases, bacterial culture is insufficiently sensitive and confirmation of tb post-mortem is neither feasible nor desirable. in this context, there is ... | 2013 | 24171845 |
| access channel residues ser315 and asp137 in mycobacterium tuberculosis catalase-peroxidase (katg) control peroxidatic activation of the pro-drug isoniazid. | peroxidatic activation of the anti-tuberculosis pro-drug isoniazid by mycobacterium tuberculosis catalase-peroxidase (katg) is regulated by gating residues of a heme access channel. the steric restriction at the bottleneck of this channel is alleviated by replacement of residue asp137 with ser, according to crystallographic and kinetic studies. | 2013 | 24185282 |
| high degree of multi-drug resistance and hetero-resistance in pulmonary tb patients from punjab state of india. | line probe assays (lpas) have been recommended for rapid screening of mdr-tb. aims of this study were (1) to compare the performance of lpa with standard bactec mgit 960 system and (2) to ascertain the pattern of genetic mutations in the resistance isolates. in phase i, a total of 141 mycobacterium tuberculosis isolates from our routine laboratory were tested by lpa and bactec mgit 960 for dst. in phase ii, 578 sputum specimens of suspected dr-tb patients were received from the punjab state of i ... | 2014 | 24184256 |
| the mutations of katg and inha genes of isoniazid-resistant mycobacterium tuberculosis isolates in taiwan. | the isoniazid (inh) resistance of mycobacterium tuberculosis is caused by mutations in the katg and inha genes encoding for catalase-peroxidase and inha, respectively. sequences of the katg and inha gene of 70 isolates were analyzed to identify the mutations and to compare the mutations with their related susceptibilities. | 2015 | 24184004 |
| [evaluation of multidrug-resistant tuberculosis treatment in ivory coast from 2008 to 2010]. | this is a retrospective study conducted from january 2008 to december 2010 on sectional descriptive analysis of records of patients treated for mdr-tb and whose follow-up was in the thoracic department of centre hospitalier universitaire (chu) of cocody in abidjan côte d'ivoire. we selected eight patients who met the inclusion criteria of 21 mdr-tb patients registered during the study period. the average age was 29.25years ranging from 21 to 39. males accounted for 75% of the patients (6 males a ... | 2013 | 24183291 |
| unmasking subtle differences in the infectivity of microevolved mycobacterium tuberculosis variants coinfecting the same patient. | clonal variants of mycobacterium tuberculosis can emerge as a result of microevolution phenomena. the functional significance of these subtle genetic rearrangements is normally disregarded. we show that clonal variants from two patients had different infective behaviours in some in vitro cellular infection models but not in others. microevolution may have a subtle impact on infectivity, but specific experimental conditions are needed to unmask it. | 2013 | 24183098 |
| tuberculosis and non-diabetic hyperglycemia: a challenge to public health management. | diabetes and tuberculosis comorbidity is an emerging challenge for public health management. but the diagnostic criteria for diabetes is based on microvascular complications of diabetes and does not reflect the level of hyperglycemia which affects infectivity of mycobacterium tuberculosis. therefore the study protocols focussing on these comorbidities need to be reviewed. | 2013 | 24182869 |
| primary splenic tubercular abscess in an immunocompromised patient-rapid diagnosis by line probe assay. | diagnosing extra-pulmonary tuberculosis is a challenge that can confound even the most practiced clinicians as clinical manifestations are vague, non-specific and typical chest radiograph findings may not be evident till late in the disease. conventional methods for mycobacteriological culture and drug susceptibility testing are slow and cumbersome. novel technologies for rapid detection of mycobacterium tuberculosis and its anti-tb drug resistance have therefore become a priority hence with the ... | 2013 | 24179920 |
| novel camphane-based anti-tuberculosis agents with nanomolar activity. | a series of new amidoalcohols and amidodiols were designed on the base of the camphor scaffold and evaluated for their in vitro activity against mycobacterium tuberculosis h37rv and mdr strain 43. some of the new compounds show 25 times higher activity than the classical anti-tb drug ethambutol. small structural changes in the side chain shift the activity from micromolar to nanomolar inhibitory concentrations. quantitative structure-activity relationship (qsar) model is derived to guide the fur ... | 2013 | 24177364 |
| rapd discloses high molecular diversity of mycobacterium tuberculosis from michoacan, mexico. | random amplified polymorphism dna (rapd) is an easy, inexpensive technique for the characterization of pathogens in low-income countries. in this study we used rapd to assess the genetic diversity of a small collection of isolates of mycobacteria from the mexican state of michoacan. in contrast with the low annual tuberculosis incidence in michoacan relative to the national average, we found a high molecular diversity value suggesting high population diversity of m. tuberculosis in the studied r ... | 2013 | 24177304 |
| liquid chromatographic determination of cpzen-45, a novel anti-tubercular drug, in biological samples. | cpzen-45 is a new drug candidate being considered for the treatment of tuberculosis (tb). the aim of this study was to develop and validate a reverse-phase high-performance liquid chromatographic (hplc) method suitable to determine cpzen-45 concentrations in biological samples. cpzen-45 was extracted from biological fluids and tissues (plasma, lung and spleen from guinea pig) by sequential extraction with acetonitrile and quantified by a waters hplc alliance system coupled with a zorbax bonus-rp ... | 2014 | 24176740 |
| simplified deoxypropionate acyl chains for mycobacterium tuberculosis sulfoglycolipid analogues: chain length is essential for high antigenicity. | | 2013 | 24174158 |
| pulmonary m. tuberculosis infection delays th1 immunity via immunoadaptor dap12-regulated irak-m and il-10 expression in antigen-presenting cells. | interaction of mycobacteria with the host leads to retarded expression of t helper cell type 1 (th1) immunity in the lung. however, the immune mechanisms remain poorly understood. using in vivo and in vitro models of mycobacterium tuberculosis (m. tb) infection, we find the immunoadaptor dap12 (dnax-activating protein of 12 kda) in antigen-presenting cells (apcs) to be critically involved in this process. upon infection of apcs, dap12 is required for irak-m (interleukin-1 receptor-associated kin ... | 2014 | 24172845 |
| mycobacterium tuberculosis infection in rhesus monkeys (macaca mulatta) and evaluation of esat-6 and cfp10 as immunodiagnostic antigens. | tuberculosis (tb) in nonhuman primates is a serious menace to the welfare of the animals and human who come into contact with them, while the rapid, accurate, and robust diagnosis is challenging. in this study, we first sought to establish an appropriate primate tb model resembling natural tb in nonhuman primates. four rhesus monkeys (macaca mulatta) of chinese origin were infected intratracheally with two low doses of m. tuberculosis h37rv. regardless of the infectious doses, all monkeys were d ... | 2013 | 24172192 |
| discordance across several methods for drug susceptibility testing of drug-resistant mycobacterium tuberculosis isolates in a single laboratory. | given the increases in drug-resistant tuberculosis, laboratory capacities for drug susceptibility testing are being scaled up worldwide. a laboratory must decide among several endorsed methodologies. we evaluated 87 mycobacterium tuberculosis isolates for concordance of susceptibility results across six methods: the l-j proportion method, mgit 960 sire ast, gene/xpert mtb/rif, genotype mtbdrplus line probe assay, mycotb mic plate, and a laboratory-developed mycobacteriophage quantitative pcr (qp ... | 2014 | 24172155 |
| proposal of a consensus set of hypervariable mycobacterial interspersed repetitive-unit-variable-number tandem-repeat loci for subtyping of mycobacterium tuberculosis beijing isolates. | mycobacterium tuberculosis beijing strains represent targets of special importance for molecular surveillance of tuberculosis (tb), especially because they are associated with spread of multidrug resistance in some world regions. standard 24-locus mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (miru-vntr) typing lacks resolution power for accurately discriminating closely related clones that often compose beijing strain populations. therefore, we evaluated a set of 7 ad ... | 2014 | 24172154 |
| mazf6 toxin of mycobacterium tuberculosis demonstrates antitoxin specificity and is coupled to regulation of cell growth by a soj-like protein. | molecular programs employed by mycobacterium tuberculosis (mtb) for the establishment of non-replicating persistence (nrp) are poorly understood. in order to investigate mechanisms regulating entry into nrp, we asked how cell cycle regulation is linked to downstream adaptations that ultimately result in nrp. based on previous reports and our recent studies, we reason that, in order to establish nrp, cells are halted in the cell cycle at the point of septum formation by coupled regulatory mechani ... | 2013 | 24172039 |
| [comparison of the data of computed tomography and bacteriological studies in the complicated forms of primary tuberculosis in children and adolescents]. | to establish the causes of bacterial excretion in complicated primary tuberculosis in children and adolescents, by comparing the data of computed tomography and the results of bacteriological studies. | 2016 | 25702438 |
| [synthesis and biological properties of α-thymidine 5'-aryl phosphonates]. | the interaction of cdi-activated diethyl phosphonoacetate with methyl 4-aminobenzoat or 3,5-difluoromethylphenylamine followed by treatment with me3sibr in dmf led to n-aryl aminocarbonylmethyl phosphonates and their ethyl esters. their coupling with 3'-acetyl-α-thymidine followed by removal of the acetyl groups gave (α-d-thymidine-5'-il) n-[4-(methoxycarbonyl-, aminocarbonyl- and carboxy)phenyl]-aminocarbonylmethyl phosphonates, (α-d-thymidine-5'-il)-[3,5-bis(trifluoromethyl)phenylaminocarbonyl ... | 2015 | 25696933 |
| tuberculosis infection in primary sjögren's syndrome: a nationwide population-based study. | primary sjögren's syndrome (pss) is a systemic autoimmune disease and may complicate with interstitial lung disease. the risk of mycobacterium tuberculosis (tb) infection in patients with pss has not been determined. this nationwide population-based study aimed to explore the incidence and risk factors of tb infection in patients with pss. we identified 4,822 pss patients from the taiwan national health insurance database and compared the incidence rates of tb infection in these patients with 48 ... | 2014 | 24170112 |
| vitamin d deficiency in patients with tuberculosis. | to determine the frequency and association of vitamin d deficiency in patients with tuberculosis. | 2013 | 24169384 |
| design, synthesis and evaluation of 6-(4-((substituted-1h-1,2,3-triazol-4-yl)methyl)piperazin-1-yl)phenanthridine analogues as antimycobacterial agents. | focus in this letter is made to design and synthesize a series of nineteen new 6-(4-((substituted-1h-1,2,3-triazol-4-yl)methyl)piperazin-1-yl)phenanthridine analogues employing click chemistry and evaluated for their anti-tubercular activity against mycobacterium tuberculosis h37rv. among the tested compounds, 7f and 7 j exhibited good activity (mic = 3.125 μg/ml), while 8a displayed excellent activity (mic = 1.56 μg/ml) against the growth of m. tuberculosis h37rv. in addition, 7f, 7 j and 8a co ... | 2013 | 24169232 |
| detection of tuberculosis in hiv-infected and -uninfected african adults using whole blood rna expression signatures: a case-control study. | a major impediment to tuberculosis control in africa is the difficulty in diagnosing active tuberculosis (tb), particularly in the context of hiv infection. we hypothesized that a unique host blood rna transcriptional signature would distinguish tb from other diseases (od) in hiv-infected and -uninfected patients, and that this could be the basis of a simple diagnostic test. | 2013 | 24167453 |
| mycobacterial dormancy regulon protein rv2623 as a novel biomarker for the diagnosis of latent and active tuberculous meningitis. | the present study was designed to investigate rv2623 antigen, a major dormancy regulon protein of mycobacterium tuberculosis (mtb) in csf of suspected latent and active tuberculous meningitis (tbm) patients. a total of 100 csf samples from tbm (n = 31), suspected latent tbm (n = 22), and suitable noninfectious control subjects (n = 47) were collected and evaluated for rv2623 antigen level using elisa protocol. a significantly high (p < 0.05) mean absorbance was observed in samples of suspected l ... | 2013 | 24167379 |
| immunocytochemical detection of mycobacterial antigen in extrapulmonary tuberculosis. | the aim of the study is to determine whether immunostaining for mycobacterial antigen can contribute to the cytological diagnosis of extrapulmonary tuberculosis (eptb). the study was carried out on aspirated material of lymph nodes, and other accessible sites, from 65 patients with clinical diagnosis of tuberculosis (tb). twenty patients, diagnosed by fine-needle aspiration, with non-tuberculous granulomas served as controls. the diagnosis of tb was based on the demonstration of acid-fast bacill ... | 2014 | 24166859 |
| prevalence and molecular characterization of fluoroquinolone-resistant mycobacterium tuberculosis isolates in china. | china is one of the countries with the highest burdens of multidrug-resistant (mdr) and fluoroquinolone (fq)-resistant tuberculosis (tb) globally. nevertheless, knowledge about the prevalence and molecular characterization of fq-resistant mycobacterium tuberculosis isolates from this region remains scant. in this study, 138 m. tuberculosis isolates determined by the agar proportion susceptibility method to be resistant to ofloxacin (ofx) were enrolled from a national drug resistance survey of ch ... | 2014 | 24165186 |
| mechanism-based inhibition of hsad: a c-c bond hydrolase essential for survival of mycobacterium tuberculosis in macrophage. | mycobacterium tuberculosis remains the leading cause of death by a bacterial pathogen worldwide. increasing prevalence of multidrug-resistant organisms means prioritizing identification of targets for antituberculars. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (hsad), part of the cholesterol metabolism operon, is vital for survival within macrophage. the c-c bond hydrolase, hsad, has a serine protease-like catalytic triad. we tested a range of serine protease and esterase inhibitors for ... | 2014 | 24164668 |
| synthetic thioamide, benzimidazole, quinolone and derivatives with carboxylic acid and ester moieties: a strategy in the design of antituberculosis agents. | synthetic heterocyclic compounds have remarkable potential activity against diseases; thioamides, benzimidazoles, quinolones and derivatives with carboxylic acid and esters moieties have shown excellent activity against mycobacterium tuberculosis. we reviewed antituberculosis activities of above compounds with reference to half maximal inhibitory concentration, minimum inhibitory concentration and structural-activity relationship which clearly indicate that electron-withdrawing groups are the ma ... | 2014 | 24164193 |
| rapid detection of pyrazinamide resistant mycobacterium tuberculosis by high resolution melting curve analysis. | to develop a high-resolution melting curve analysis (hrm) assay for detection of pza resistance. | 2013 | 24163999 |
| [cutaneous manifestation of tuberculosis causing impaired healing after minor injury]. | after a trivial injury, a male patient from india presented with a non-healing minor injury. a necrotic lesion at the right medial ankle was evident. | 2013 | 24163165 |
| selman a. waksman, winner of the 1952 nobel prize for physiology or medicine. | the history of the discovery and development of streptomycin is reviewed here from the personal standpoint of a member of dr. selman waksman's antibiotic screening research team. the team approach of eight individuals illustrates how the gradual enhancement of the screening methodology was developed. i illustrate three study periods with key aspects in the development of streptomycin which led to a nobel prize being granted to professor waksman. one item not previously emphasized is the employme ... | 2014 | 24162573 |
| serial lobar lung lavage in pulmonary alveolar proteinosis. | pulmonary alveolar proteinosis (pap) is a rare disease with worldwide distribution and an estimated incidence of 0.36 cases per million. we report a case of a pap coexisting with pneumocystis jiroveci pneumonia and mycobacterium tuberculosis infection. the patient was treated with serial lobar lung lavages, gm-csf, cotrimoxazole, and antituberculosis drugs. his pao2 on room air improved from 45.7 to 63.8 torr and pulmonary functions normalized (fvc 81.2%, fev1 95.3%, fev1/fvc 91.8). a high-resol ... | 2013 | 24162118 |
| kinetic and mechanistic characterization of the glyceraldehyde 3-phosphate dehydrogenase from mycobacterium tuberculosis. | glyceraldehyde 3-phosphate dehydrogenase (gapdh) is a glycolytic protein responsible for the conversion of glyceraldehyde 3-phosphate (g3p), inorganic phosphate and nicotinamide adenine dinucleotide (nad(+)) to 1,3-bisphosphoglycerate (1,3-bpg) and the reduced form of nicotinamide adenine dinucleotide (nadh). here we report the characterization of gapdh from mycobacterium tuberculosis (mtb). this enzyme exhibits a kinetic mechanism in which first nad(+), then g3p bind to the active site resultin ... | 2013 | 24161676 |
| statistical evaluation of two microbiological diagnostic methods of pulmonary tuberculosis after implementation of a directly observed treatment short-course program. | to evaluate the diagnostic accuracy of smear and culture tests of clinical samples of pulmonary tuberculosis after the introduction of the directly observed treatment short-course (dots) program. | 2013 | 24159529 |
| measuring of mycobacterium tuberculosis growth. a correlation of the optical measurements with colony forming units. | the quantification of colony forming units (cfu), turbidity, and optical density at 600 nm (od600) measurements were used to evaluate mycobacterium tuberculosis growth. turbidity and od600 measurements displayed similar growth curves, while cfu quantification showed a continuous growth curve. we determined the cfu equivalents to mcfarland and od600 units. | 2013 | 24159318 |
| comparison of resazurin microtiter assay performance and bactec mgit 960 in the susceptibility testing of brazilian clinical isolates of mycobacterium tuberculosis to four first-line drugs. | we assessed the performance of rema in comparison with bactec mgit 960 in the susceptibility testing of 80 mycobacterium tuberculosis clinical isolates from clemente ferreira institute against four drugs. rema proved to be a rapid and accurate method, providing excellent correlation with bactec mgit 960, with the exception of results for the ethambutol drug. | 2013 | 24159317 |
| human gene variants linked to enhanced nlrp3 activity limit intramacrophage growth of mycobacterium tuberculosis. | activation of the nlrp3 inflammasome and subsequent generation of interleukin 1β is initiated in macrophages upon recognition of several stimuli. in the present work, we show that gain-of-function gene variants of inflammasome components known to predispose individuals to inflammatory disorders have a host-protective role during infection with mycobacterium tuberculosis. by isolation of macrophages from patients and healthy blood donors with genetic variants in nlrp3 and card8 and subsequent inf ... | 2014 | 24158955 |
| inhibition studies on mycobacterium tuberculosis n-acetylglucosamine-1-phosphate uridyltransferase (glmu). | peptidoglycan is an essential component of the cell wall of bacteria, including mycobacterium tuberculosis, that provides structural strength and rigidity to enable internal osmotic pressure to be withstood. the first committed step in the biosynthesis of peptidoglycan involves the formation of uridine diphosphate-n-acetylglucosamine (udp-glcnac) from uridine triphosphate (utp) and glcnac-1-phosphate. this reaction is catalysed by n-acetylglucosamine-1-phosphate uridyltransferase (glmu), a bifun ... | 2013 | 24158720 |
| provisional cdc guidelines for the use and safety monitoring of bedaquiline fumarate (sirturo) for the treatment of multidrug-resistant tuberculosis. | multidrug-resistant tuberculosis (mdr tb) is caused by mycobacterium tuberculosis that is resistant to at least isoniazid and rifampin, the two most effective of the four first-line tb drugs (the other two drugs being ethambutol and pyrazinamide). mdr tb includes the subcategory of extensively drug-resistant tb (xdr tb), which is mdr tb with additional resistance to any fluoroquinolone and to at least one of three injectable anti-tb drugs (i.e., kanamycin, capreomycin, or amikacin). mdr tb is di ... | 2013 | 24157696 |
| lipid bilayer preparations of membrane proteins for oriented and magic-angle spinning solid-state nmr samples. | solid-state nmr spectroscopy has been used successfully for characterizing the structure and dynamics of membrane proteins as well as their interactions with other proteins in lipid bilayers. such an environment is often necessary for achieving native-like structures. sample preparation is the key to this success. here we present a detailed description of a robust protocol that results in high-quality membrane protein samples for both magic-angle spinning and oriented-sample solid-state nmr. the ... | 2013 | 24157546 |
| a new unifying theory of the pathogenesis of tuberculosis. | it is set in stone that mycobacterium tuberculosis is a facultative intracellular bacterial parasite. this axiom drives our knowledge of the host response, the way we design vaccines against the organism by generating protective t cells, and to a lesser extent, the way we try to target anti-microbial drugs. the purpose of this article is to commit total heresy. i believe that m. tuberculosis can equally well be regarded as an extracellular pathogen and may in fact spend a large percentage of its ... | 2014 | 24157189 |
| sulfonamides incorporating fluorine and 1,3,5-triazine moieties are effective inhibitors of three β-class carbonic anhydrases from mycobacterium tuberculosis. | a new series of fluorine containing 1,3,5-triazinyl sulfonamide derivatives obtained from cyanuric fluoride, sulfanilamide/4-aminoethylbenzenesulfonamide followed and incorporating also amin0, amino alcohol and amino acid moieties have been investigated as inhibitors of three β-carbonic anhydrases (cas, ec 4.2.1.1) from the bacterial pathogen mycobacterium tuberculosis, mtca1 (rv1284), mtca 2 (rv3588c) and mtca 3 (rv3273). all three enzymes were efficiently inhibited by these sulfonamides with k ... | 2014 | 24156740 |
| the esx-3 secretion system is necessary for iron and zinc homeostasis in mycobacterium tuberculosis. | esx-3 is one of the five type vii secretion systems encoded by the mycobacterium tuberculosis genome. we recently showed the essentiality of esx-3 for m. tuberculosis viability and proposed its involvement in iron and zinc metabolism. in this study we confirmed the role of esx-3 in iron uptake and its involvement in the adaptation to low zinc environment in m. tuberculosis. moreover, we unveiled functional differences between the esx-3 roles in m. tuberculosis and m. smegmatis showing that in th ... | 2013 | 24155985 |
| discovery of mycobacterium tuberculosis protein tyrosine phosphatase b (ptpb) inhibitors from natural products. | protein tyrosine phosphatase b (ptpb) is one of the virulence factors secreted into the host cell by mycobacterium tuberculosis. ptpb attenuates host immune defenses by interfering with signal transduction pathways in macrophages and, therefore, it is considered a promising target for the development of novel anti-tuberculosis drugs. here we report the discovery of natural compound inhibitors of ptpb among an in house library of more than 800 natural substances by means of a multidisciplinary ap ... | 2013 | 24155919 |
| significant reduction in rate of indeterminate results of the quantiferon-tb gold in-tube test by shortening incubation delay. | the quantiferon-tb gold in-tube (qft-g it) test (cellestis inc., valencia, ca) is one of the gamma interferon release assays (igras) that are promising tools for diagnosing active or latent mycobacterium tuberculosis infections. we investigated the clinical and laboratory factors that affect the rate of indeterminate qft-g it test results. we also suggest a workflow strategy for achieving optimized test results using the qft-g it test for the diagnosis of active tuberculosis (tb) or latent tb in ... | 2014 | 24153122 |
| pre-multidrug-resistant mycobacterium tuberculosis beijing strain associated with disseminated tuberculosis in a pet dog. | resistance to isoniazid, ethambutol, and streptomycin was detected in a mycobacterium tuberculosis strain, belonging to the beijing family lineage, isolated from two nodule exudates of a yorkshire terrier with generalized tuberculosis. this report alerts medical practitioners to the risk of dissemination of pre-multidrug-resistant tuberculosis (premdr-tb) through exposure to m. tuberculosis-shedding pets. | 2014 | 24153119 |
| long-incubation-time gamma interferon release assays in response to purified protein derivative, esat-6, and/or cfp-10 for the diagnosis of mycobacterium tuberculosis infection in children. | the diagnosis of childhood active tuberculosis (atb) and latent mycobacterium tuberculosis (m. tuberculosis) infection (ltbi) remains a challenge, and the replacement of tuberculin skin tests (tst) with commercialized gamma interferon (ifn-γ) release assays (igra) is not currently recommended. two hundred sixty-six children between 1 month and 15 years of age, 214 of whom were at risk of recent m. tuberculosis infection and 51 who were included as controls, were prospectively enrolled in our stu ... | 2014 | 24152386 |
| 'cough-triggered' tuberculosis screening among adults with diabetes in tanzania. | diabetes increases the risk of tuberculosis and the prevalence of diabetes is rising in tuberculosis-endemic regions such as sub-saharan africa. resource-appropriate strategies for tuberculosis case finding among african adults with diabetes are needed. the aims of this study were to determine prevalence of tuberculosis and evaluate one screening strategy among adult tanzanians with diabetes. | 2014 | 24152037 |
| tuberculosis transmission among immigrants and autochthonous populations of the eastern province of saudi arabia. | eastern province of saudi arabia is an industrial zone with large immigrant population and high level of tuberculosis case notification among immigrants. the impact of immigration and current trends of tuberculosis transmission among immigrants and autochthonous population in the region had not been investigated so far using molecular tools. | 2013 | 24147042 |
| first evaluation after implementation of a quality control system for the second line drug susceptibility testing of mycobacterium tuberculosis joint efforts in low and high incidence countries. | three networks/projects involving 27 european countries were established to investigate the quality of second-line drug (sld) susceptibility testing with conventional and molecular methods. 1. the "baltic-nordic tb-laboratory network" comprised 11 reference laboratories in the baltic-nordic states. they performed sld testing in the first phase with a panel of 20 mycobacterium tuberculosis strains. after several laboratories made technical changes a second panel of 10 strains with a higher propor ... | 2013 | 24146924 |
| validation of biomarkers for distinguishing mycobacterium tuberculosis from non-tuberculous mycobacteria using gas chromatography-mass spectrometry and chemometrics. | tuberculosis (tb) remains a major international health problem. rapid differentiation of mycobacterium tuberculosis complex (mtb) from non-tuberculous mycobacteria (ntm) is critical for decisions regarding patient management and choice of therapeutic regimen. recently we developed a 20-compound model to distinguish between mtb and ntm. it is based on thermally assisted hydrolysis and methylation gas chromatography-mass spectrometry and partial least square discriminant analysis. here we report t ... | 2013 | 24146846 |
| therapeutic efficacy of sq641-ne against mycobacterium tuberculosis. | a phospholipid-based nanoemulsion formulation of sq641 (sq641-ne) was active against intracellular mycobacterium tuberculosis in j774a.1 mouse macrophages, although sq641 by itself was not. intravenous (i.v.) sq641-ne was cleared from circulation and reached peak concentrations in lung and spleen in 1 h. in a murine tuberculosis (tb) model, 8 i.v. doses of sq641-ne at 100 mg/kg of body weight over 4 weeks caused a 1.73 log10 cfu reduction of m. tuberculosis in spleen and were generally bacterios ... | 2014 | 24145521 |
| occurrence of rpob mutations in isoniazid-resistant but rifampin-susceptible mycobacterium tuberculosis isolates from germany. | four out of 143 phenotypically isoniazid-resistant but rifampin-susceptible mycobacterium tuberculosis strains that were isolated from patients in germany in 2011 had mutations in the rifampin resistance-determining region of rpob. after performing drug susceptibility testing (dst) with two methods, the proportion method on löwenstein-jensen medium and using the bactec 960 mycobacteria growth indicator tube system, we conclude that the two methods are equally reliable for phenotypic dst and mic ... | 2014 | 24145520 |
| nitrite produced by mycobacterium tuberculosis in human macrophages in physiologic oxygen impacts bacterial atp consumption and gene expression. | in high enough concentrations, such as produced by inducible nitric oxide synthase (inos), reactive nitrogen species (rns) can kill mycobacterium tuberculosis (mtb). lesional macrophages in macaques and humans with tuberculosis express inos, and mice need inos to avoid succumbing rapidly to tuberculosis. however, mtb's own ability to produce rns is rarely considered, perhaps because nitrate reduction to nitrite is only prominent in axenic mtb cultures at oxygen tensions ≤1%. here we found that c ... | 2013 | 24145454 |
| mycobacterium tuberculosis possesses an unusual tmrna rescue system. | trans-translation is a key process in bacteria which recycles stalled ribosomes and tags incomplete nascent proteins for degradation. this ensures the availability of ribosomes for protein synthesis and prevents the accumulation of dysfunctional proteins. the tmrna, ssra, is responsible for both recovering stalled ribosomes and encodes the degradation tag; ssra associates and functions with accessory proteins such as smpb. although ssra and smpb are ubiquitous in bacteria, they are not essential ... | 2014 | 24145139 |
| characterization of w-beijing isolates of mycobacterium tuberculosis from the western cape. | the purpose of this simple study was to characterize a panel of clinical isolates of mycobacterium tuberculosis obtained from the western cape region of south africa where new clinical vaccine trials are beginning, in the low dose aerosol guinea pig infection model. most of the strains tested grew well in the lungs and other organs of these animals, and in most cases gave rise to moderate to very severe lung damage. we further observed that the current bcg vaccine was highly protective against t ... | 2013 | 24144471 |
| risk factors for pulmonary tuberculosis in croatia: a matched case-control study. | mycobacterium tuberculosis is a necessary, but not sufficient, cause of tuberculosis. a number of studies have addressed the issue of risk factors for tuberculosis development. croatia is a european country with an incidence rate of 14/100 000 which is slowly decreasing. the aim of this study is to evaluate the potential demographic, socioeconomic, behavioural and biological risk factors for tuberculosis in croatia in comparison to other high-income, low-incidence european countries. | 2013 | 24144113 |
| fusing dual-event data sets for mycobacterium tuberculosis machine learning models and their evaluation. | the search for new tuberculosis treatments continues as we need to find molecules that can act more quickly, be accommodated in multidrug regimens, and overcome ever increasing levels of drug resistance. multiple large scale phenotypic high-throughput screens against mycobacterium tuberculosis (mtb) have generated dose response data, enabling the generation of machine learning models. these models also incorporated cytotoxicity data and were recently validated with a large external data set. a c ... | 2013 | 24144044 |
| estimated rate of reactivation of latent tuberculosis infection in the united states, overall and by population subgroup. | we estimated the rate of reactivation tuberculosis (tb) in the united states, overall and by population subgroup, using data on tb cases and mycobacterium tuberculosis isolate genotyping reported to the centers for disease control and prevention during 2006-2008. the rate of reactivation tb was defined as the number of non-genotypically clustered tb cases divided by the number of person-years at risk for reactivation due to prevalent latent tb infection (ltbi). ltbi was ascertained from tubercul ... | 2014 | 24142915 |
| identification of crucial amino acids of bacterial peptide deformylases affecting enzymatic activity in response to oxidative stress. | peptide deformylase (pdf) is an essential bacterial metalloprotease involved in deformylation of n-formyl group from nascent polypeptide chains during protein synthesis. iron-containing variants of this enzyme from salmonella enterica serovar typhimurium (spdf) and mycobacterium tuberculosis (mpdf), although inhibited by oxidizing agents like h2o2, exhibited strikingly different 50% inhibitory concentrations (ic50s) that ranged from nanomolar (spdf) to millimolar (mpdf) levels. furthermore, the ... | 2014 | 24142250 |
| availability of an assay for detecting mycobacterium tuberculosis, including rifampin-resistant strains, and considerations for its use - united states, 2013. | in august 2013, the food and drug administration (fda) permitted marketing of the xpert mtb/rif assay (cepheid, sunnyvale, california) to detect dna of the mycobacterium tuberculosis complex (mtbc) and genetic mutations associated with resistance to rifampin (rmp) in unprocessed sputum and concentrated sputum sediments. along with clinical, radiographic, and other laboratory findings, results of the assay aid in the diagnosis of pulmonary tuberculosis (tb). the assay is a nucleic acid amplificat ... | 2013 | 24141407 |
| design, chemical synthesis of 3-(9h-fluoren-9-yl)pyrrolidine-2,5-dione derivatives and biological activity against enoyl-acp reductase (inha) and mycobacterium tuberculosis. | we report here the discovery, synthesis and screening results of a series of 3-(9h-fluoren-9-yl)pyrrolidine-2,5-dione derivatives as a novel class of potent inhibitors of mycobacterium tuberculosis h37rv strain as well as the enoyl acyl carrier protein reductase (enr) inha. among them, several compounds displayed good activities against inha which is one of the key enzymes involved in the type ii fatty acid biosynthesis pathway of the mycobacteria cell wall. furthermore, some exhibited promising ... | 2013 | 24140915 |
| binding of mgtr, a salmonella transmembrane regulatory peptide, to mgtc, a mycobacterium tuberculosis virulence factor: a structural study. | mgtr, a highly hydrophobic peptide expressed in salmonella enterica serovar typhimurium, inhibits growth in macrophages through binding to the membrane protein mgtc that has been identified as essential for replication in macrophages. while the mycobacterium tuberculosis mgtc is highly homologous to its s. typhi analogue, there does not appear to be an mtb homologue for mgtr, raising significant pharmacological interest in this system. here, solid-state nmr and epr spectroscopy in lipid bilayer ... | 2014 | 24140750 |
| optimization of sabre for polarization of the tuberculosis drugs pyrazinamide and isoniazid. | hyperpolarization produces nuclear spin polarization that is several orders of magnitude larger than that achieved at thermal equilibrium thus providing extraordinary contrast and sensitivity. as a parahydrogen induced polarization (phip) technique that does not require chemical modification of the substrate to polarize, signal amplification by reversible exchange (sabre) has attracted a lot of attention. using a prototype parahydrogen polarizer, we polarize two drugs used in the treatment of tu ... | 2013 | 24140625 |
| iqg-607 abrogates the synthesis of mycolic acids and displays intracellular activity against mycobacterium tuberculosis in infected macrophages. | in this work, the antitubercular activity of a pentacyano(isoniazid)ferrate(ii) compound (iqg-607) was investigated using a macrophage model of mycobacterium tuberculosis infection. importantly, treatment of m.-tuberculosis-infected macrophages with iqg-607 significantly diminished the number of cfu compared with the untreated control group. the antitubercular activity of iqg-607 was similar to that observed for the positive control drugs isoniazid and rifampicin. nevertheless, higher concentrat ... | 2014 | 24139881 |
| two rare-class tricyclic diterpenes with antitubercular activity from the caribbean sponge svenzea flava. application of vibrational circular dichroism spectroscopy for determining absolute configuration. | two new natural products, 3 and 4, and their predecessor 7-isocyanoisoneoamphilecta-1(14),15-diene (2), of the rare isoneoamphilectane class of marine diterpenes, along with the known amphilectane diterpenes 6-8, were isolated from the n-hexane extract of the marine sponge svenzea flava collected at great inagua island, bahamas. the molecular structures of compounds 3 and 4 were established by spectroscopic (1d/2d nmr, ir, uv, hrms) methods and confirmed by a series of chemical correlation studi ... | 2013 | 24138557 |
| clinical value of a whole blood interferon-γ release assay for the diagnosis of mycobacterium tuberculosis infection during antitubercular treatment. | the aim of this study was to evaluate the sensitivity and specificity of a whole blood interferon-γ release assay, the quantiferon(®)-tb gold in-tube (qft-git) test, in the diagnosis of mycobacterium tuberculosis (mtb) infection, and to assess its monitoring role during antitubercular treatment. in total, 20 patients received the qft test, along with other commonly-used tests, prior to, and following, 2- and 6-month courses of antitubercular treatment; the results were compared and statistically ... | 2013 | 24137207 |
| matrix metalloproteinases and tissue damage in hiv-tuberculosis immune reconstitution inflammatory syndrome. | the hiv-tb-associated immune reconstitution inflammatory syndrome (tb-iris) can complicate combined treatments for hiv-1 and tb. little is known about tissue damage in tb-iris. matrix metalloproteinases (mmps) degrade components of the extracellular matrix and consequently may play a role in such immunopathology. here we investigated the involvement of mmps in tb-iris. we determined mmp transcript abundance and secreted protein in mycobacterium tuberculosis stimulated pbmcs from 22 tb-iris patie ... | 2014 | 24136296 |
| effect of isoniazid therapy for latent tb infection on quantiferon-tb gold in-tube responses in adults with positive tuberculin skin test results in a high tb incidence area: a controlled study. | t-cell interferon-γ release assays (igras) are used in the diagnosis of mycobacterium tuberculosis infection and could be useful biomarkers of response to treatment of latent tb infection for clinical trials, infection control units, and tb programs. | 2014 | 24135768 |
| g-quadruplex-generating polymerase chain reaction for visual colorimetric detection of amplicons. | we have developed a self-reporting polymerase chain reaction (pcr) system for visual colorimetric gene detection and distinction of single nucleotide polymorphisms (snps). amplification is performed using target-specific primers modified with a 5'-end tail that is complementary to a g-quadruplex deoxyribozyme-forming sequence. at end-point, g-quadruplexes are forced to fold from pcr-generated duplex dna and then are used to colorimetrically report the successful occurrence of pcr by assaying the ... | 2014 | 24135653 |
| upconversion nanoparticle-based förster resonance energy transfer for detecting the is6110 sequence of mycobacterium tuberculosis complex in sputum. | upconversion nanoparticles (ucnps), which are excited at near-infrared wavelength (980 nm), emit high-energy photons. since ucnps display a high signal-to-noise ratio and no photobleaching, they are extremely useful for diagnostic application. in this study, we applied ucnps for detecting the is6110 sequence of the mycobacterium tuberculosis complex (mtbc) and evaluated the feasibility of the system for use in molecular diagnostics. using biotinylated primers, is6110 dna pcr was performed and th ... | 2014 | 24135541 |
| cd4(+) cd25(high) forkhead box protein 3(+) regulatory t lymphocytes suppress interferon-γ and cd107 expression in cd4(+) and cd8(+) t cells from tuberculous pleural effusions. | tuberculous pleural effusion is characterized by a t helper type 1 (th1) profile, but an excessive th1 response may also cause tissue damage that might be controlled by regulatory mechanisms. in the current study we investigated the role of regulatory t cells (treg ) in the modulation of th1 responses in patients with tuberculous (tb) pleurisy. using flow cytometry we evaluated the proportion of treg (cd4(+) cd25(high) forkhead box protein 3(+) ), interferon (ifn)-γ and interleukin (il)-10 expre ... | 2014 | 24134738 |
| long-term dominance of mycobacterium tuberculosis uganda family in peri-urban kampala-uganda is not associated with cavitary disease. | previous studies have shown that mycobacterium tuberculosis (mtb) uganda family, a sub-lineage of the mtb lineage 4, is the main cause of tuberculosis (tb) in uganda. using a well characterized patient population, this study sought to determine whether there are clinical and patient characteristics associated with the success of the mtb uganda family in kampala. | 2013 | 24134504 |
| evaluation of rezasurin microtiter assay and high resolution melting curve analysis for detection of rifampicin and isoniazid resistance of mycobacterium tuberculosis clinical isolates. | several methods have been proposed for rapid detection of drug resistance of mycobacterium tuberculosis (m. tuberculosis). high resolution melting (hrm) curve analysis has been developed for accurate and simultaneous detection of resistance of m. tuberculosis isolates to rifampicin (rif) and isoniazid (inh). other techniques include the resazurin microtiter assay (rema) which is one of the new colorimetric phenotypic methods. the present study evaluated the hrm curve analysis and rema assay in c ... | 2013 | 24133904 |
| statin therapy reduces the mycobacterium tuberculosis burden in human macrophages and in mice by enhancing autophagy and phagosome maturation. | statins are cholesterol-lowering drugs, targeting hmg-coa reductase, thereby reducing the risk of coronary disorders and hypercholesterolemia. however, they also can influence immunologic responses. | 2014 | 24133190 |
| combining computational methods for hit to lead optimization in mycobacterium tuberculosis drug discovery. | tuberculosis treatments need to be shorter and overcome drug resistance. our previous large scale phenotypic high-throughput screening against mycobacterium tuberculosis (mtb) has identified 737 active compounds and thousands that are inactive. we have used this data for building computational models as an approach to minimize the number of compounds tested. | 2014 | 24132686 |
| non-tuberculous mycobacterial infection of the parotid gland in an immunocompetent elderly patient. | we experienced an extremely rare case in which combined antibacterial therapy for a non-tuberculous mycobacteria (ntm) infection of the parotid gland achieved a favourable outcome in an elderly immunocompetent patient. although a 79-year-old man, who presented with swelling and fistula formation in the left parotid gland region, initially received combined antituberculous therapy due to a positive result of acid-fast staining, the lesion did not respond to these agents. thereafter, since the cul ... | 2013 | 24132446 |
| evaluation of agar-based medium with sheep sera for testing of drug susceptibility of mycobacterium tuberculosis to isoniazid, rifampin, ethambutol, and streptomycin. | the performance of sheep sera instead of sheep blood in agar-based media was investigated for susceptibility testing of mycobacterium tuberculosis against primary drugs. the levels of agreement between agar-based medium supplemented with sheep sera and the proportion method on middlebrook 7h11 agar as the reference method for determining susceptibility to isoniazid (inh), rifampin (rif), ethambutol (emb), and streptomycin (str) were 98.4, 98.4, 95.3, and 100%, respectively. | 2013 | 24131699 |
| role of pnca and rpsa gene sequencing in detection of pyrazinamide resistance in mycobacterium tuberculosis isolates from southern china. | we sequenced pnca and rpsa genes plus flanking regions of 161 mycobacterium tuberculosis isolates and found 10 new pnca and 3 novel rpsa mutations in pyrazinamide-resistant strains determined by the bactec mgit 960 system. the 3' end of rpsa might be added as the target of molecular detection of pyrazinamide susceptibility. | 2014 | 24131688 |