| autophagy and the evasion of host defense: a new variation on the theme for burkholderia cepacia? | | 2011 | 21997365 |
| parallel bacterial evolution within multiple patients identifies candidate pathogenicity genes. | bacterial pathogens evolve during the infection of their human host(1-8), but separating adaptive and neutral mutations remains challenging(9-11). here we identify bacterial genes under adaptive evolution by tracking recurrent patterns of mutations in the same pathogenic strain during the infection of multiple individuals. we conducted a retrospective study of a burkholderia dolosa outbreak among subjects with cystic fibrosis, sequencing the genomes of 112 isolates collected from 14 individuals ... | 2011 | 22081229 |
| a proteome reference map of the causative agent of melioidosis burkholderia pseudomallei. | burkholderia pseudomallei is the etiologic agent of melioidosis. using 2de and maldi-tof ms, we report here a proteome reference map constructed from early stationary phase, a bacterial adaptation process. we identified 282 protein spots representing 220 orfs; many of them have been implicated in bacterial pathogenesis. up to 20% of identified orfs belong to post-translational modification and stress responses. the proteome reference map will support future analysis of the bacterial gene and env ... | 2011 | 21960737 |
| Identification, origin, and evolution of leaf nodulating symbionts of Sericanthe (Rubiaceae). | Bacterial leaf symbiosis is an intimate association between bacteria and plants in which endosymbionts are housed within leaf nodules. This phenomenon has been reported in three genera of Rubiaceae (Pavetta, Psychotria, and Sericanthe), but the bacterial partner has only been identified in Psychotria and Pavetta. Here we report the identification of symbiotic bacteria in two leaf nodulating Sericanthe species. Using 16S rRNA data and common housekeeping genetic markers (recA and gyrB) we studied ... | 2011 | 22203556 |
| Total Synthesis and Stereochemical Assignment of Burkholdac B, a Depsipeptide HDAC Inhibitor. | Three diastereomers of burkholdac B were prepared by total synthesis, enabling the full stereochemical assignment of the natural product. It is proposed that burkholdac B is identical to thailandepsin A independently isolated by Cheng from the same strain of Burkholderia thailandensis . Burkholdac B is the most potent among depsipeptide histone deacetylase inhibitors in growth inhibition of the MCF7 breast cancer cell line with an IC(50) of 60 pM. | 2011 | 22091906 |
| Genetic Diversity of Endophytic Diazotrophs of the Wild Rice, Oryza alta and Identification of the New Diazotroph, Acinetobacter oryzae sp. nov. | Thirty-three endophytic diazotrophs were isolated from surface-sterilized leaves, stem, and roots of wild rice Oryza alta. The SDS-PAGE profile of total protein and insertion sequence-based polymerase chain reaction (IS-PCR) fingerprinting grouped the isolates into four clusters (I-IV). The 16S rRNA gene sequence homology of the representative strains B21, B31, B1, and B23 of clusters I, II, III, and IV were assigned to Pseudomonas oleovorans (99.2% similarity), Burkholderia fungorum (99.4% simi ... | 2011 | 22105517 |
| biochemical, structural and molecular dynamics analyses of the potential virulence factor ripa from yersinia pestis. | human diseases are attributed in part to the ability of pathogens to evade the eukaryotic immune systems. a subset of these pathogens has developed mechanisms to survive in human macrophages. yersinia pestis, the causative agent of the bubonic plague, is a predominately extracellular pathogen with the ability to survive and replicate intracellularly. a previous study has shown that a novel rip (required for intracellular proliferation) operon (ripa, ripb and ripc) is essential for replication an ... | 2011 | 21966419 |
| Antimicrobial resistance to ceftazidime involving loss of penicillin-binding protein 3 in Burkholderia pseudomallei. | Known mechanisms of resistance to ß-lactam antibiotics include ß-lactamase expression, altered drug target, decreased bacterial permeability, and increased drug efflux. Here, we describe a unique mechanism of ß-lactam resistance in the biothreat organism Burkholderia pseudomallei (the cause of melioidosis), associated with treatment failure during prolonged ceftazidime therapy of natural infection. Detailed comparisons of the initial ceftazidime-susceptible infecting isolate and subsequent cefta ... | 2011 | 21969582 |
| Ionic-surfactant-coated Burkholderia cepacia lipase as a highly active and enantioselective catalyst for the dynamic kinetic resolution of secondary alcohols. | | 2011 | 21954139 |
| [Burkholderia cepacia bacteremia: a prospective analysis of 33 episodes]. | Introduction: The aim of this study is to describe clinical characteristics and outcome of Burkholderia cepacia bacteraemia, susceptibility of the isolates and differences between cases from epidemic outbreaks and sporadic cases. Material and methods: From 1993 to 2009, episodes of B. cepacia bacteraemia were prospectively collected in a university hospital. Results: A total of 33 episodes were included, of which 21 were part of two outbreaks (9 in 1994 and 12 in 2006). Outbreak cases had a med ... | 2011 | 22173191 |
| Microwave-assisted enzymatic synthesis of beef tallow biodiesel. | Optimal conditions for the microwave-assisted enzymatic synthesis of biodiesel have been developed by a full 2(2) factorial design leading to a set of seven runs with different combinations of molar ratio and temperature. The main goal was to reduce the reaction time preliminarily established by a process of conventional heating. Reactions yielding biodiesel, in which beef tallow and ethanol used as raw materials were catalyzed by lipase from Burkholderia cepacia immobilized on silica-PVA and mi ... | 2011 | 22120648 |
| Alteration in cell surface properties of Burkholderia spp. during surfactant-aided biodegradation of petroleum hydrocarbons. | Chemical surfactants may impact microbial cell surface properties, i.e., cell surface hydrophobicity (CSH) and cell surface charge, and may thus affect the uptake of components from non-aqueous phase liquids (NAPLs). This work explored the impact of Triton X-100, Igepal CA 630, and Tween 80 (at twice the critical micelle concentration, CMC) on the cell surface characteristics of Burkholderia cultures, Burkholderia cepacia (ES1, aliphatic degrader) and Burkholderia multivorans (NG1, aromatic degr ... | 2011 | 22089390 |
| Two Norwegian patients with melioidosis presenting with bacteraemia and splenic and prostatic abscesses. | Infections caused by Burkholderia pseudomallei are rare in nonendemic areas, such as Scandinavia. We report the first two cases of melioidosis in Norway presenting with bacteraemia and splenic and prostatic abscesses, respectively. | 2011 | 22017720 |
| Structural basis of the substrate specificity of bifunctional isocitrate dehydrogenase kinase/phosphatase. | Isocitrate dehydrogenase kinase/phosphatase (AceK) regulates entry into the glyoxylate bypass by reversibly phosphorylating isocitrate dehydrogenase (ICDH). On the basis of the recently determined structure of the AceK-ICDH complex from Escherichia coli, we have classified the structures of homodimeric NADP(+)-ICDHs to rationalize and predict which organisms likely contain substrates for AceK. One example is Burkholderia pseudomallei (Bp). Here we report a crystal structure of Bp-ICDH that exhib ... | 2011 | 21870819 |
| A Burkholderia pseudomallei macrophage infectivity potentiator-like protein has rapamycin-inhibitable peptidylprolyl isomerase activity and pleiotropic effects on virulence. | Macrophage infectivity potentiators (Mips) are a group of virulence factors encoded by pathogenic bacteria such as Legionella, Chlamydia, and Neisseria species. Mips are part of the FK506-binding protein (FKBP) family, whose members typically exhibit peptidylprolyl cis-trans isomerase (PPIase) activity which is inhibitable by the immunosuppressants FK506 and rapamycin. Here we describe the identification and characterization of BPSS1823, a Mip-like protein in the intracellular pathogen Burkholde ... | 2011 | 21859853 |
| Occidiofungin's Chemical Stability and In vitro Potency Against Candida species. | Occidiofungin is a cyclic glyco-lipopeptide produced by Burkholderia contaminans. Minimum inhibitory concentrations (MICs) against Candida species were between 0.5 and 1.0 µg/mL. Occidiofungin retains its in vitro potency in the presence of 5% and 50% human serum with a minimal lethal concentration (MLC) of 2 and 4 µg/mL, respectively. Time-kill and post-antifungal effect (PAFE) experiments of occidiofungin against Candida albicans were measured. These results demonstrate that occidiofungin is f ... | 2011 | 22106210 |
| in vitro antimicrobial studies of silver carbene complexes: activity of free and nanoparticle carbene formulations against clinical isolates of pathogenic bacteria. | silver carbenes may represent novel, broad-spectrum antimicrobial agents that have low toxicity while providing varying chemistry for targeted applications. here, the bactericidal activity of four silver carbene complexes (sccs) with different formulations, including nanoparticles (nps) and micelles, was tested against a panel of clinical strains of bacteria and fungi that are the causative agents of many skin and soft tissue, respiratory, wound, blood, and nosocomial infections. | 2012 | 21972270 |
| [Detection and identification of highly pathogenic bacteria within the framework of the EQADeBa project--Part I: Samples containing living pathogens]. | Bacillus anthracis, Yersinia pestis, Francisella tularensis, Brucella sp., Bulkholderia mallei are B. pseudomallei are highly pathogenic bacteria of potential bioterrorism risk. To support the early warning and rapid response capacity to ensure an effective reaction to bioterrorist attacks the international project "Establishment of Quality Assurances for Detection of Highly Pathogenic Bacteria of Potential Bioterrorism Risk" (EQADeBa) has been established. The aim of the project was establishme ... | 2011 | 22184939 |
| extractive fermentation for improved production and recovery of lipase derived from burkholderia cepacia using a thermoseparating polymer in aqueous two-phase systems. | an extractive fermentation technique was developed using a thermoseparating reagent to form a two-phase system for simultaneous cell cultivation and downstream processing of extracellular burkholderia cepacia lipase. a 10% (w/w) solution of ethylene oxide-propylene oxide (eopo) with a molecular mass of 3900g/mol and ph 8.5, a 200rpm speed, and 30°c were selected as the optimal conditions for lipase production (55u/ml). repetitive batch fermentation was performed by continuous replacement of the ... | 2011 | 22061444 |
| bopc is a type iii secreted effector protein of burkholderia pseudomallei. | burkholderia pseudomallei, the causative agent of melioidosis, exploits the bsa type iii secretion system (t3ss) to deliver effector proteins into host cells. these effectors manipulate host cell functions; thus, contributing to the ability of the bacteria to evade the immune response and cause disease. only two bsa-secreted effectors have been conclusively identified to date. here, we report the identification of the third b. pseudomallei type iii secreted effector protein, designated bopc. bop ... | 2011 | 22092682 |
| genetic diversity of mimosa pudica rhizobial symbionts in soils of french guiana: investigating the origin and diversity of burkholderia phymatum and other beta-rhizobia. | the genetic diversity of 221 mimosa pudica bacterial symbionts trapped from eight soils from diverse environments in french guiana was assessed by 16s rrna pcr-rflp, rep-pcr fingerprints, as well as by phylogenies of their 16s rrna and reca housekeeping genes, and by their nifh, noda and nodc symbiotic genes. interestingly, we found a large diversity of beta-rhizobia, with burkholderia phymatum and burkholderia tuberum being the most frequent and diverse symbiotic species. other species were als ... | 2011 | 22093060 |
| identification of circulating bacterial antigens by in vivo microbial antigen discovery. | detection of microbial antigens in clinical samples can lead to rapid diagnosis of an infection and administration of appropriate therapeutics. a major barrier in diagnostics development is determining which of the potentially hundreds or thousands of antigens produced by a microbe are actually present in patient samples in detectable amounts against a background of innumerable host proteins. in this report, we describe a strategy, termed in vivo microbial antigen discovery (inmad), that we used ... | 2011 | 21846829 |
| In Vitro Antibacterial Activity of Sphaeropsidins and Chemical Derivatives toward Xanthomonas oryzae pv. oryzae, the Causal Agent of Rice Bacterial Blight. | Sphaeropsidin A, the main phytotoxin produced by Diplodia cupressi, as well as the two natural analogues sphaeropsidins B and C and 14 derivatives obtained by chemical modifications were assayed for antibacterial activity against Xanthomonas oryzae pv. oryzae, Pseudomonas fuscovaginae, and Burkholderia glumae, the causal agents of severe bacterial rice diseases. The results showed a strong and specific activity of sphaeropsidin A against X. oryzae pv. oryzae, while no activity was observed again ... | 2011 | 22124378 |
| Symbiotic interaction of endophytic bacteria with arbuscular mycorrhizal fungi and its antagonistic effect on Ganoderma boninense. | Endophytic bacteria (Pseudomonas aeruginosa UPMP3 and Burkholderia cepacia UMPB3), isolated from within roots of oil palm (Elaeis guineensis Jacq.) were tested for their presymbiotic effects on two arbuscular mcorrhizal fungi, Glomus intraradices UT126 and Glomus clarum BR152B). These endophytic bacteria were also tested for antagonistic effects on Ganoderma boninense PER 71, a white wood rot fungal pathogen that causes a serious disease in oil palm. Spore germination and hyphal length of each a ... | 2011 | 21887636 |
| antimicrobial and antibiofilm activity of ll-37 and its truncated variants against burkholderia pseudomallei. | the gram-negative bacterium burkholderia pseudomallei is the aetiological agent of melioidosis, which is an endemic disease in tropical areas of southeast asia and northern australia. burkholderia pseudomallei has intrinsic resistance to a number of commonly used antibiotics and has also been reported to develop a biofilm. resistance to killing by antimicrobial agents is one of the hallmarks of bacteria grown in biofilm. the aim of this study was to determine the antimicrobial activity and mecha ... | 2012 | 22005071 |
| delayed brain abscess after embolization of arterio-venous malformation: report of two cases and review of literature. | infections of central nervous system are rare complications of endovascular procedures. review of literature revealed only four reported cases of brain abscesses after embolization of intracranial arteriovenous malformations (avms). we report two new cases of delayed brain abscess after embolization of avm. in one of the patients, it was due to an unusual organism, burkholderia caeci. | 2011 | 21891947 |
| Profiling of Burkholderia cepacia secretome at mid-logarithmic and early-stationary phases of growth. | Burkholderia cepacia is a Gram-negative pathogen that causes serious respiratory infections in immunocompromised patients and individuals with cystic fibrosis. This bacterium is known to release extracellular proteins that may be involved in virulence. | 2011 | 22046299 |
| Evolution of catabolic pathways and their regulatory systems in synthetic nitroaromatic compounds degrading bacteria. | Evolution of catabolic pathways for the degradation of synthetic nitroaromatic compounds is currently ongoing process because these compounds have been in nature only for a short time. Bacteria isolated from contaminated areas contain pathways for the degradation of nitroaromatic compounds at different stages of progression. Therefore, the emergence of pathways for the degradation of such chemicals provides a good opportunity to investigate evolutionary processes leading to the emergence of new ... | 2011 | 21895794 |
| molecular approaches to pathogenesis study of burkholderia cenocepacia, an important cystic fibrosis opportunistic bacterium. | burkholderia cenocepacia is a gram-negative opportunistic pathogen belonging to the burkholderia cepacia complex (bcc). it is spread in a wide range of ecological niches, and in cystic fibrosis patients, it is responsible for serious infections. its eradication is very difficult due to the high level of intrinsic resistance to clinically relevant antibiotics. one of the main resistance mechanisms in clinical isolates is represented by efflux systems that are able to extrude a variety of molecule ... | 2011 | 21997606 |
| Burkholderia cenocepacia BC2L-C is a super lectin with dual specificity and proinflammatory activity. | Lectins and adhesins are involved in bacterial adhesion to host tissues and mucus during early steps of infection. We report the characterization of BC2L-C, a soluble lectin from the opportunistic pathogen Burkholderia cenocepacia, which has two distinct domains with unique specificities and biological activities. The N-terminal domain is a novel TNF-a-like fucose-binding lectin, while the C-terminal part is similar to a superfamily of calcium-dependent bacterial lectins. The C-terminal domain d ... | 2011 | 21909279 |
| Screening microorganisms for insulin binding reveals binding by Burkholderia multivorans and Burkholderia cenocepacia and novel attachment of insulin to Aeromonas salmonicida via the A-layer. | Exposure to microorganisms is considered an environmental factor which can contribute to Type 1 diabetes. Insulin binding proteins on microorganisms may induce production of antibodies which can react with the human insulin receptor (HIR) with possible consequences in developing a diabetic autoimmune response against HIR and insulin. The interaction of insulin with microorganisms was studied by screening forty-five microbial species for their ability to bind insulin. Binding assays were performe ... | 2011 | 22171975 |
| screening of freeze-dried protective agents for the formulation of biocontrol strains, bacillus cereus ar156, burkholderia vietnamiensis b418 and pantoea agglomerans 2re40. | the effects of different freeze-drying protective agents on the viabilities of biocontrol strains bacillus cereus ar156, burkholderia vietnamiensis b418 and pantoea agglomerans 2re40 were investigated. | 2012 | 22017666 |
| Survey of antimicrobial resistance in clinical Burkholderia pseudomallei isolates over two decades in Northeast Thailand. | A 21-year survey conducted in northeast Thailand of antimicrobial resistance to parenteral antimicrobial drugs used to treat melioidosis identified 24/4,021 (0.6%) patients with one or more isolates resistant to ceftazidime (n = 8), amoxicillin-clavulanic acid (n = 4), or both drugs (n = 12). Two cases were identified at admission, and the remainder were detected a median of 15 days after starting antimicrobial therapy. Resistance to carbapenem drugs was not detected. These findings support the ... | 2011 | 21876049 |
| chronic stenotrophomonas maltophilia infection and exacerbation outcomes in cystic fibrosis. | chronic stenotrophomonas maltophilia infection is a risk factor for pulmonary exacerbation in cystic fibrosis (cf) but its impact on subsequent clinical outcomes is unknown. the aim of this study was to determine the effect of chronic s. maltophilia infection and associated antimicrobial therapy on the recovery of forced expiratory lung volume in 1s (fev(1)) following pulmonary exacerbation. | 2011 | 21849265 |
| reflectron maldi tof and maldi tof/tof mass spectrometry reveal novel structural details of native lipooligosaccharides. | lipooligosaccharides (los) are powerful gram-negative glycolipids that evade the immune system and invade host animal and vegetal cells. the structural elucidation of los is pivotal to understanding the mechanisms of infection at the molecular level. the amphiphilic nature of los has been the main obstacle for structural analysis by matrix-assisted laser desorption/ionization (maldi) mass spectrometry (ms). our approach has resolved this important issue and has permitted us to obtain reflectron ... | 2011 | 22124985 |
| Cupriavidus and Burkholderia species associated with agricultural plants that grow in alkaline soils. | The presence of Burkholderia, Cupriavidus, and Ralstonia species in northeastern Mexico was investigated. An analysis of the root surrounding soil from different agricultural plants led to the isolation of Burkholderia and Cupriavidus species but no Ralstonia strains. Most Cupriavidus species were unknown and grouped into two clusters according to ARDRA profiles. The 16S rRNA sequence analysis showed that the Cupriavidus isolates were highly related among them and with different Cupriavidus spec ... | 2011 | 22203548 |
| burkholderia gladioli - a predictor of poor outcome in cystic fibrosis patients who receive lung transplants? a case of locally invasive rhinosinusitis and persistent bacteremia in a 36-year-old lung transplant recipient with cystic fibrosis. | there have been very few reports describing postlung transplant outcomes in patients' infected⁄colonized with burkholderia gladioli pretransplant. a case involving a lung transplant recipient with cystic fibrosis who ultimately died as a result of severe rhinosinusitis due to b gladioli infection in the context of postlung transplant immunosuppression is reported. | 2011 | 22059186 |
| Development of vaccines against burkholderia pseudomallei. | Burkholderia pseudomallei is a Gram-negative bacterium which is the causative agent of melioidosis, a disease which carries a high mortality and morbidity rate in endemic areas of South East Asia and Northern Australia. At present there is no available human vaccine that protects against B. pseudomallei, and with the current limitations of antibiotic treatment, the development of new preventative and therapeutic interventions is crucial. This review considers the multiple elements of melioidosis ... | 2011 | 21991263 |
| the microbial ecology of wine grape berries. | grapes have a complex microbial ecology including filamentous fungi, yeasts and bacteria with different physiological characteristics and effects upon wine production. some species are only found in grapes, such as parasitic fungi and environmental bacteria, while others have the ability to survive and grow in wines, constituting the wine microbial consortium. this consortium covers yeast species, lactic acid bacteria and acetic acid bacteria. the proportion of these microorganisms depends on th ... | 2011 | 22189021 |
| a case of tricuspid valve endocarditis due to burkholderia cepacia complex. | burkholderia cepacia complex organisms are environmental gram-negative bacteria which rarely cause disease in otherwise immunocompetent individuals. we describe a case of tricuspid valve endocarditis secondary to b. cepacia complex in an injecting intravenous drug user in auckland, new zealand. | 2011 | 21952388 |
| In vitro activity of S-(3,4-dichlorobenzyl)isothiourea hydrochloride and novel structurally related compounds against multidrug-resistant bacteria, including Pseudomonas aeruginosa and Burkholderia cepacia complex. | The aim of this study was to establish the antimicrobial activities of S-(3,4-dichlorobenzyl)isothiourea hydrochloride (A22) and a series of structurally related compounds against multidrug-resistant (MDR) bacteria. The minimum inhibitory concentrations (MICs) of 21 compounds were determined against 18 strains of pathogenic bacteria in addition to Pseudomonas aeruginosa (n=19) and Burkholderia cepacia complex (BCC) (n=20) isolated from the sputa of cystic fibrosis patients. Selected compounds we ... | 2012 | 21993484 |
| 2,4-DNT removal in intimately coupled photobiocatalysis: the roles of adsorption, photolysis, photocatalysis, and biotransformation. | The removal of 2,4-dinitrotoluene (2,4-DNT) by simultaneous UV-photo(cata)lysis and biodegradation was explored using intimately coupled photolysis/photocatalysis and biodegradation (ICPB) with two novel porous carriers. First, a porous ceramic carrier was used to attach the photocatalyst (TiO(2)) on its exterior and accumulate biomass in its interior. UV irradiation alone decomposed 71% of the 2,4-DNT in 60 h, and TiO(2) catalyst improved the photolysis to 77%. Second, a macroporous sponge carr ... | 2011 | 22101782 |
| production and characteristics of the whole-cell lipase from organic solvent tolerant burkholderia sp. zyb002. | the thermostable and organic solvent tolerant whole-cell lipase (wcl) was produced by burkholderia sp. zyb002 with broad spectrum organic solvent tolerance. the production medium of the wcl was primarily optimized, which resulted in the maximum activity of 22.8 u/ml and the 5.1-fold increase of the wcl yield. the optimized culture medium was as follows (% w/v or v/v): soybean meal 2, soybean oil 0.5, manganese sulfate 0.1, k(2)hpo(4) 0.1, olive oil 0.5, initial ph 6.0, inoculum density 2, liquid ... | 2011 | 22081330 |
| Regulation of Universal Stress Protein Genes by Quorum Sensing and RpoS, and Its Role in Burkholderia glumae. | Burkholderia glumae possesses a quorum sensing (QS) system mediated by N-octanoyl-homoserine lactone (C8-HSL) and its cognate receptor TofR. TofR/C8-HSL regulates expression of a transcriptional regulator, qsmR. We identified one of the universal stress proteins (Usps), Usp2, from the genome-wide analysis of QS-dependent proteomes of B. glumae. In the whole genome of B. glumae BGR1, 11 usp genes (usp1 to 11) were identified. Among the stress conditions tested, usp1 and usp2 mutants died 1 h afte ... | 2011 | 22178971 |
| Proteomics Analyses of the Opportunistic Pathogen Burkholderia vietnamiensis Using Protein Fractionations and Mass Spectrometry. | The main objectives of this work were to obtain a more extensive coverage of the Burkholderia vietnamiensis proteome than previously reported and to identify virulence factors using tandem mass spectrometry. The proteome of B. vietnamiensis was precipitated into four fractions to as extracellular, intracellular, cell surface and cell wall proteins. Two different approaches were used to analyze the proteins. The first was a gel-based method where 1D SDS-PAGE was used for separation of the protein ... | 2011 | 22187530 |
| Characterization of DC1, a broad host range Bcep22-like podovirus. | Bcep22-like phages are a recently described group of podoviruses that infect strains of Burkholderia cenocepacia. We have isolated and characterized a novel member of this group named DC1. This podovirus shows many genomic similarities to BcepIL02 and Bcep22, but infects strains belonging to multiple Burkholderia cepacia complex (BCC) species. | 2011 | 22139000 |
| diabetes does not influence activation of coagulation, fibrinolysis or anticoagulant pathways in gram-negative sepsis (melioidosis). | diabetes is associated with a disturbance of the haemostatic balance and is an important risk factor for sepsis, but the influence of diabetes on the pathogenesis of sepsis remains unclear. melioidosis ( burkholderia pseudomallei infection) is a common cause of community-acquired sepsis in southeast asia and northern australia. we sought to investigate the impact of pre-existing diabetes on the coagulation and fibrinolytic systems during sepsis caused by b.pseudomallei . we recruited a cohort of ... | 2011 | 21979378 |
| Role of NADPH Oxidase versus Neutrophil Proteases in Antimicrobial Host Defense. | NADPH oxidase is a crucial enzyme in mediating antimicrobial host defense and in regulating inflammation. Patients with chronic granulomatous disease, an inherited disorder of NADPH oxidase in which phagocytes are defective in generation of reactive oxidant intermediates (ROIs), suffer from life-threatening bacterial and fungal infections. The mechanisms by which NADPH oxidase mediate host defense are unclear. In addition to ROI generation, neutrophil NADPH oxidase activation is linked to the re ... | 2011 | 22163282 |
| resolution of 2-nitroalcohols by burkholderia cepacia lipase-catalyzed enantioselective acylation. | racemic 2-nitro-1-phenylethanol was resolved by via enantioselective transesterification catalyzed by burkholderia cepacia lipase. the reaction afforded excellent e values (e > 200) and enantioselectivity (up to >99% enantiomeric excesses [ee]) of both remaining substrates and acetylated product. moreover, the lipase displayed high enantioselectivity in the resolution of additional 2-nitroalcohols (e up to >200). this method provides an efficient alternative for obtaining enantiopure 2-nitroalco ... | 2012 | 21972142 |
| facile syntheses of the disaccharide repeating unit of the o-antigenic polysaccharide of burkholderia pseudomallei strain 304b and its dimer and trimer. | a highly efficient strategy for the preparation of a disaccharide-repeating unit of the o-antigenic polysaccharide of burkholderia pseudomallei strain 304b, and its dimer and trimer, has been developed through a regio- and stereoselective manner using p-methoxylphenyl 2,4,6-tri-o-benzoyl-α-d-glucopyranoside and 3-o-allyloxycarbonyl-2,4-di-o-benzoyl-6-deoxy-α-l-talopyranosyl trichloroacetimidate as the key synthons. the target molecules were equipped with a p-methoxylphenyl handle at the reducing ... | 2011 | 21963340 |
| maldi-tof ms improves routine identification of non-fermenting gram negative isolates from cystic fibrosis patients. | identification of non-fermenting gram-negative bacteria (nfgnb) from cystic fibrosis (cf) patients is often limited. a collection of stored nfgnb isolates (n=182) recovered from cf patients over a 15year period was examined. the routinely reported identification during this period was compared with that obtained by maldi-tof ms. isolates giving discrepant identification at the genus level were further analyzed by 16s rdna sequencing. the maldi-tof ms system identified 94% of the isolates, includ ... | 2011 | 21968086 |
| risk factors for mortality in patients with burkholderia cepacia complex bacteraemia. | over the last 2 decades, burkholderia cepacia complex has emerged as a serious human pathogen, especially in critically ill patients. b. cepacia complex has been associated with increased morbidity and mortality in intensive care unit patients. however, in our literature search, we could not find studies on risk factors for mortality in patients with b. cepacia complex bacteraemia. therefore, we investigated risk factors for mortality in b. cepacia complex bacteraemia. | 2011 | 21888567 |
| hydrophobic surface induced activation of pseudomonas cepacia lipase immobilized into mesoporous silica. | lipase from pseudomonas cepacia (pcl) was successfully immobilized into siliceous mesocellular foams (mcfs) with various hydrophobic/hydrophilic surfaces. the catalytic performances of immobilized pcl were investigated using the transesterification reaction and hydrolytic reaction as model reactions. the specific activity of immobilized pcl greatly increased with enhanced surface hydrophobicity of mcfs, mainly because of lipase activation via hydrophobic interaction between alkyl groups in mcfs ... | 2011 | 21851086 |
| insight into the metabolism of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (ddt) by biphenyl dioxygenases. | in this work we have investigated the ability of the biphenyl dioxygenase of burkholderia xenovorans lb400 (bphae(lb400)) and of pandoraea pnomenusa b356 (bphae(b356)) to metabolize ddt. data show bphae(lb400) is unable to metabolize this substrate but bphae(b356) metabolizes ddt to produce two stereoisomers. structural analysis of ddt-docked bphae(lb400) and bphae(b356) identified residue phe336 of bphae(lb400) as critical to prevent productive binding of ddt to bphae(lb400). furthermore, the f ... | 2011 | 22001737 |
| characterization and inference of gene gain/loss along burkholderia evolutionary history. | a comparative analysis of 60 complete burkholderia genomes was conducted to obtain insight in the evolutionary history behind the diversity and pathogenicity at species level. a concatenated multiprotein phyletic pattern and a dataset with burkholderia clusters of orthologous genes (bucogs) were constructed. the extent of horizontal gene transfer (hgt) was assessed using a markov based probabilistic method. a reconstruction of the gene gains and losses history shows that more than half of the bu ... | 2011 | 22084562 |
| epidemiological tracking and population assignment of the non-clonal bacterium, burkholderia pseudomallei. | rapid assignment of bacterial pathogens into predefined populations is an important first step for epidemiological tracking. for clonal species, a single allele can theoretically define a population. for non-clonal species such as burkholderia pseudomallei, however, shared allelic states between distantly related isolates make it more difficult to identify population defining characteristics. two distinct b. pseudomallei populations have been previously identified using multilocus sequence typin ... | 2011 | 22180792 |
| the fucose-binding lectin from opportunistic pathogen burkholderia ambifaria binds to both plant and human oligosaccharidic epitopes. | burkholderia ambifaria is generally associated with the rhizosphere of plants where it has biocontrol effects on other microorganisms. it is also a member of the burkhoderia cepacia complex, a group of closely related bacteria that causes lung infections in immunocompromised patients, as well as patients with granulomatous disease or cystic fibrosis. our previous work indicated that fucose on human epithelia is a frequent target for lectins and adhesins of lung pathogens (sulak et al., plos path ... | 2011 | 22170069 |
| towards a rapid molecular diagnostic for melioidosis: comparison of dna extraction methods from clinical specimens. | optimising dna extraction from clinical samples for burkholderia pseudomallei type iii secretion system real-time pcr in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. direct testing on blood remains problematic; testing dna extracted from plasma was superior to dna from whole blood or buffy coat. | 2012 | 22108495 |
| autophagy stimulation by rapamycin suppresses lung inflammation and infection by burkholderia cenocepacia in a model of cystic fibrosis. | cystic fibrosis (cf) is the most common inherited lethal disease of caucasians which results in multi organ dysfunction. however, 85% of the deaths are due to pulmonary infections. infection by burkholderia cenocepacia (b. cepacia) is a particularly lethal threat to cf patients because it causes severe and persistent lung inflammation and is resistant to nearly all available antibiotics. in cftr δf508 mouse macrophages, b. cepacia persists in vacuoles that do not fuse with the lysosomes and medi ... | 2011 | 21997369 |
| the phylogenetic distribution and ecological role of carbon monoxide oxidation in the genus burkholderia. | burkholderia is a physiologically and ecologically diverse genus that occurs commonly in assemblages of soil and rhizosphere bacteria. although burkholderia is known for its heterotrophic versatility, we demonstrate that 14 distinct environmental isolates oxidized carbon monoxide (co) and possessed the gene encoding the catalytic subunit of form i co dehydrogenase (coxl). dna from a burkholderia isolate obtained from a passalid beetle also contained coxl as do the genomic sequences of species h1 ... | 2011 | 22029898 |
| delayed activation of host innate immune pathways in streptozotocin-induced diabetic hosts leads to more severe disease during infection with burkholderia pseudomallei. | diabetes mellitus is a predisposing factor of melioidosis contributing to higher mortality rates in diabetics infected with burkholderia pseudomallei. to investigate how diabetes alters the inflammatory response, we established a streptozotocin (stz)-induced diabetic murine acute-phase melioidosis model. viable b. pseudomallei cells were consistently detected in the blood, liver and spleen during the 42 hr course of infection but the hyperglycemic environment did not increase the bacterial burde ... | 2011 | 22136109 |
| composition and antimicrobial activity of the essential oil of heracleum thomsonii (clarke) from the cold desert of the western himalayas. | volatile oil composition of hydro-distilled (hd) and supercritical carbon dioxide (sc-co(2)) essential oil of freshly collected aerial parts of heracleum thomsonii (umbeliferae) from the western himalayas was studied by gc-fid and gc-ms. results revealed qualitative and quantitative dissimilarity in the composition of hydro-distilled and sc-co(2) extracted oils. nineteen constituents, which accounted for 89.32% of total constituents in hd oil, represented by limonene (4.31%), (z)-β-ocimene (3.69 ... | 2011 | 21854172 |
| burkholderia pseudomallei: an uncommon cause of bacteraemic pneumonia in a diabetic. | | 2011 | 22128627 |
| out of the ground: aerial and exotic habitats of the melioidosis bacterium burkholderia pseudomallei in grasses in australia. | melioidosis is an emerging infectious disease of humans and animals in the tropics caused by the soil bacterium burkholderia pseudomallei. despite high fatality rates, the ecology of b. pseudomallei remains unclear. we used a combination of field and laboratory studies to investigate b. pseudomallei colonization of native and exotic grasses in northern australia. multivariable and spatial analyses were performed to determine significant predictors for b. pseudomallei occurrence in plants and soi ... | 2011 | 22176696 |
| microbiological spectrum of brain abscess at a tertiary care hospital in south india: 24-year data and review. | intracranial abscesses are life-threatening infections that pose a diagnostic challenge not only to the neurosurgeon but also to the microbiologists. detailed studies documenting the spectrum of infecting agents involved in brain abscesses are limited from india. materials and methods. this is a retrospective analysis of 352 samples from 1987 to 2010 analyzed at a tertiary care hospital in south india from 1987 to 2010, to document the changing trends with time. results. the age of the patients ... | 2011 | 22191080 |
| bioremediation of carbofuran contaminated soil under saturated condition: soil column study. | disturbed soil columns, 5.8 cm in diameter and 25 cm in length, were used as a basic model to simulate the movement of carbofuran in rice field soil under saturated conditions. bioaugmentation using a specific carbofuran degrader, burkholderia sp. pcl3, in free and immobilized cell forms and biostimulation using rice straw as organic amendment were applied with the aim of enhancing the degradation of carbofuran in soil and to prevent the movement of carbofuran along with the flow through. in the ... | 2011 | 22173656 |
| growth and cesium uptake responses of phytolacca americana linn. and amaranthus cruentus l. grown on cesium contaminated soil to elevated co2 or inoculation with a plant growth promoting rhizobacterium burkholderia sp. d54, or in combination. | growth and cesium uptake responses of plants to elevated co(2) and microbial inoculation, alone or in combination, can be explored for clean-up of contaminated soils, and this induced phytoextraction may be better than the natural process. the present study used open-top chambers to investigate combined effects of burkholderia sp. d54 inoculation and elevated co(2) (860 μl l(-1)) on growth and cs uptake by phytolacca americana and amaranthus cruentus grown on soil spiked with various levels of c ... | 2011 | 22074893 |
| Isolation, characterization and docking studies of 2,3-dihydroxybiphenyl 1,2-dioxygenase from an activated sludge metagenome. | A 2,3-dihydroxybiphenyl-1,2-dioxygenase gene (designated as bphC_meta) was identified in activated sludge metagenome by PCR. This gene shared 99% sequence identity with BphC from Burkholderia xenovorans LB400. The enzyme was purified from recombinant Escherichia coli with a subunit molecular mass of 32 ± 1 kDa. It was optimally active at pH 9.0 and 40°C, using 2,3-dihydroxybiphenyl as a substrate. Activity toward substituted catechols was: 2,3-dihydroxybiphenyl > 3-methylcatechol > catechol > 4- ... | 2012 | 21898128 |
| exposing the third chromosome of burkholderia cepacia complex strains as a virulence plasmid. | the burkholderia cepacia complex (bcc) consists of 17 closely related species of opportunistic bacterial pathogens, which are particularly problematic for cystic fibrosis patients and immunocompromised individuals. bcc genomes consist of multiple replicons, and each strain sequenced to date has three chromosomes. in addition to genes thought to be essential for survival, each chromosome carries at least one rrna operon. we isolated three mutants during a transposon mutagenesis screen that were n ... | 2011 | 22171913 |
| adaptation mechanisms of bacteria during the degradation of polychlorinated biphenyls in the presence of natural and synthetic terpenes as potential degradation inducers. | in this study, we examined the effect of polychlorinated biphenyls (pcbs) in the presence of natural and synthetic terpenes and biphenyl on biomass production, lipid accumulation, and membrane adaptation mechanisms of two pcb-degrading bacterial strains pseudomonas stutzeri and burkholderia xenovorans lb400. according to the results obtained, it could be concluded that natural terpenes, mainly those contained in ivy leaves and pine needles, decreased adaptation responses induced by pcbs in these ... | 2011 | 22159613 |
| comparative evaluation of three commercially available complement fixation test antigens for the diagnosis of glanders. | the sensitivity and specificity of three commercially available complement fixation test (cft) antigens from c.c.pro (c.c.pro), central veterinary institute of wageningen ur (cidc) and the united states department of agriculture (usda) were comparatively evaluated by testing 410 sera collected from glanders-endemic and non-endemic areas (200 true-negative randomly collected sera and 210 sera collected from experimentally immunised animals (12 rabbits, 19 horses), clinically positive (135) and cu ... | 2011 | 21896565 |
| plasminogen activator inhibitor type i contributes to protective immunity during experimental gram-negative sepsis (melioidosis). | melioidosis is a frequent cause of sepsis in southeast asia caused by the gram-negative bacterium burkholderia pseudomallei. patients with melioidosis have elevated circulating levels of plasminogen activator inhibitor type 1 (pai-1), an important regulator of inflammation and fibrinolysis. | 2011 | 21848642 |
| [pandoraea sputorum colonization in a patient with cystic fibrosis]. | cystic fibrosis (cf) is a disease produced by a defect in the transmembrane conductance regulator protein, cftr. currently, the morbidity and mortality associated with cf are fundamentally related with the lung affectation that is a consequence of this defect. with the progression of the disease, there is an increase in the isolation of non-fermenting gram-negative bacilli colonizing these patients. the genus pandoraea arises from a reclassification of species included within the "burkholderia c ... | 2011 | 21908092 |
| inhibition of cullin ring ligases by cycle inhibiting factor: evidence for interference with nedd8-induced conformational control. | cycle inhibiting factor (cif) is produced by pathogenic intracellular bacteria and injected into the host cells via a type iii secretion system. cif is known to interfere with the eukaryotic cell cycle by inhibiting the function of cullin ring e3 ubiquitin ligases (crls). cullin proteins form the scaffold protein of crls and are modified with the ubiquitin-like protein nedd8, which exerts important conformational control required for crl activity. cif has recently been shown to catalyze the deam ... | 2011 | 21903097 |
| biodiesel synthesis and conformation of lipase from burkholderia cepacia in room temperature ionic liquids and organic solvents. | biodiesel synthesis and conformation of burkholderia cepacia lipase (bcl) were studied in 19 different room temperature ionic liquids (rtlls) with a range of cation and anion structures. overall, anion selection had a greater influence on biodiesel conversion than cation choice. rtils containing tf2n- and pf6- anions were suitable reaction media, while rtil of [ompy][bf4] was the best reaction medium with a biodiesel yield of 82.2±1.2%. rtils with strong water miscible properties showed very low ... | 2011 | 21955878 |
| diagnostic assays for glanders. | | 2011 | 22184353 |
| characterization of the volatile profile of antarctic bacteria by using solid-phase microextraction-gas chromatography-mass spectrometry. | bacteria belonging to the burkholderia cepacia complex (bcc) are significant pathogens in cystic fibrosis (cf) patients and are resistant to a plethora of antibiotics. in this context, microorganisms from antarctica are interesting because they produce antimicrobial compounds inhibiting the growth of other bacteria. this is particularly true for bacteria isolated from antarctic sponges. the aim of this work was to characterize a set of antarctic bacteria for their ability to produce new natural ... | 2011 | 22012672 |
| biotic and abiotic experimental identification of bacterial influence on calcium isotopic signatures. | in this study, we tested experimentally the influence of plant and bacterial activities on the calcium (ca) isotope distribution between soil solutions and plant organs. abiotic apatite weathering experiments were performed under two different ph conditions using mineral and organic acids. biotic experiments were performed using either apatite or ca-enriched biotite substrates in the presence of scots pines, inoculated or not with the rhizosphere bacterial strain bulkholderia glathei pml1(12), o ... | 2011 | 21913253 |
| protective antigens against glanders identified by expression library immunization. | burkholderia are highly evolved gram-negative bacteria that primarily infect solipeds but are transmitted to humans by ingestion and cutaneous or aerosol exposures. heightened concern over human infections of burkholderia mallei and the very closely related species b. pseudomallei is due to the pathogens' proven effectiveness as bioweapons, and to the increased potential for natural opportunistic infections in the growing diabetic and immuno-compromised populations. these burkholderia species ar ... | 2011 | 22125550 |
| intravenous immunoglobulin treatment for macrophage activation syndrome complicating chronic granulomatous disease. | objectives: chronic granulomatous disease is a rare phagocyte disorder characterized by an increased susceptibility to infections and inflammatory complications. we describe two patients with chronic granulomatous disease (cgd) complicated by macrophage activation syndrome (mas) (secondary hemophagocytic lymphohistiocytosis) treated with intravenous immunoglobulin (ivig). methods: a report of two cases of cgd com ... | 2011 | 22119934 |
| biological control of botrytis cinerea using the antagonistic and endophytic burkholderia cepacia cs5 for vine plantlet protection. | antifungal activity of the burkholderia cepacia cs5 was tested in vitro and in vivo for the control of botrytis cinerea . bacterial biomass was significantly improved by the amendment of znso(4), mo(7)(nh(4))(6)o(24), and mannitol to the nby medium; consequently, the amount of the secreted fungicides was increased. the quantification of b. cinerea inhibition, in liquid and solid conditions, showed an important sensitivity of this fungus to the strain cs5 fungicides. microscopic monitoring impact ... | 2011 | 22004162 |
| polyphasic approach for the characterization of rhizobial symbionts effective in fixing n(2) with common bean (phaseolus vulgaris l.). | common bean (phaseolus vulgaris l.) is a legume that has been reported as highly promiscuous in nodulating with a variety of rhizobial strains, often with low effectiveness in fixing nitrogen. the aim of this work was to assess the symbiotic efficiency of rhizobial strains isolated from common bean seeds, nodules of arachis hypogaea, mucuna pruriens, and soils from various brazilian agroecosystems, followed by the characterization of elite strains identified in the first screening. forty-five el ... | 2011 | 22159885 |
| identification of differentially expressed proteins from burkholderia pseudomallei isolated during primary and relapsing melioidosis. | burkholderia pseudomallei causes septicemic melioidosis with a high rate of relapse, however microbial determinants of relapse are unknown. proteins were analyzed from sequential b. pseudomallei isolates from primary and relapsing melioidosis. analysis by isotope tagging for relative and absolute quantitation revealed that factors required for nitric oxide detoxification (hmpa) and necessary for anaerobic growth (arca, arcc and arcb) were highly expressed in the relapse isolate. two-dimensional ... | 2011 | 22172334 |
| the antibacterial properties of docosahexaenoic omega-3 fatty acid against the cystic fibrosis multi-resistant pathogen burkholderia cenocepacia. | burkholderia cepacia complex (bcc) bacteria are opportunistic pathogens that cause multiresistant pulmonary infections in cystic fibrosis (cf) patients. in this study we evaluated the in vitro antimicrobial efficacy of eight unsaturated fatty acids against burkholderia cenocepacia k56-2, a cf epidemic strain. docosahexaenoic acid (dha) was the most active compound. its action can be either bacteriostatic or bactericidal, depending upon the concentration used. the effect of dha was also evaluated ... | 2011 | 22150831 |
| comparative genomics and functional analysis of the niap family uncover nicotinate transporters from bacteria, plants, and mammals. | the transporter(s) that mediate uptake of nicotinate and its n-methyl derivative trigonelline are not known in plants, and certain mammalian nicotinate transporters also remain unidentified. potential candidates for these missing transporters include proteins from the ubiquitous niap family. in bacteria, niap genes often belong to nad-related regulons, and genetic evidence supports a role for bacillus subtilis and acinetobacter baumannii niap proteins in uptake of nicotinate or nicotinamide. oth ... | 2011 | 21953179 |
| is diversification history of maize influencing selection of soil bacteria by roots? | a wide range of plant lines has been propagated by farmers during crop selection and dissemination, but consequences of this crop diversification on plant-microbe interactions have been neglected. our hypothesis was that crop evolutionary history shaped the way the resulting lines interact with soil bacteria in their rhizospheres. here, the significance of maize diversification as a factor influencing selection of soil bacteria by seedling roots was assessed by comparing rhizobacterial community ... | 2012 | 22126532 |
| Diversity of 16S rRNA genes from bacteria of sugarcane rhizosphere soil. | Sugarcane is an important agricultural product of Brazil, with a total production of more than 500 million tons. Knowledge of the bacterial community associated with agricultural crops and the soil status is a decisive step towards understanding how microorganisms influence crop productivity. However, most studies aim to isolate endophytic or rhizosphere bacteria associated with the plant by culture-dependent approaches. Culture-independent approaches allow a more comprehensive view of entire ba ... | 2011 | 22042267 |
| Isolation and characterization of monochloroacetic acid-degrading bacteria. | Five Burkholderia strains (CL-1, CL-2, CL-3, CL-4, and CL-5) capable of degrading monochloroacetic acid (MCA) were isolated from activated sludge or soil samples gathered from several parts of Japan. All five isolates were able to grow on MCA as the sole source of carbon and energy, and argentometry and gas chromatography-mass spectroscopy analyses showed that these five strains consumed MCA completely and released chloride ions stoichiometrically within 25 h. The five isolates also grew on mono ... | 2011 | 22139456 |
| Successful Cessation of Transmitting Healthcare-Associated Infections due to Burkholderia cepacia Complex in a Neonatal Intensive Care Unit in a Japanese Children's Hospital. | Background: Burkholderia cepacia strains have been known to possess the capability to cause serious infections especially in neonatal intensive care units (NICUs), and their multi-drug resistances become a severe threat in hospital settings. The aim of this investigation was to evaluate the B. cepacia complex infections in the NICU in Nagano Children's Hospital, Azumino 399-8288, Japan, and to report the intervention leading to the successful cessation of the outbreak. - Methodology: The inciden ... | 2011 | 22112360 |
| potato cytosine methylation and gene expression changes induced by a beneficial bacterial endophyte, burkholderia phytofirmans strain psjn. | burkholderia phytofirmans strain psjn is a highly effective plant-beneficial endophyte. we have used a combination of capillary electrophoresis and methylation-sensitive amplification length polymorphism (ce-msap) analysis to investigate the potato genomic dna cytosine methylation changes that occur in response to psjn bacterization. six weeks after psjn inoculation, over 6800 loci were identified and assessed in two in vitro grown potato varieties, the strongly-responsive red pontiac and the po ... | 2012 | 22099516 |
| changes in the repertoire of natural antibodies caused by immunization with bacterial antigens. | the repertoire of natural anti-glycan antibodies in naïve chickens and in chickens immunized with bacteria burkholderia mallei, burkholderia pseudomallei, and francisella tularensis as well as with peptides from an outer membrane protein of b. pseudomallei was studied. a relatively restricted pattern of natural antibodies (first of all igy against bacterial cell wall peptidoglycan fragments, l-rha, and core n-acetyllactosamine) shrank and, moreover, the level of detectable antibodies decreased a ... | 2011 | 21999548 |
| immunogenic consensus sequence t helper epitopes for a pan-burkholderia biodefense vaccine. | background. biodefense vaccines against category b bioterror agents burkholderia pseudomallei (bpm) and burkholderia mallei (bm) are needed, as they are both easily accessible to terrorists and have strong weaponization potential. burkholderia cepaciae (bc), a related pathogen, causes chronic lung infections in cystic fibrosis patients. since bpm, bm and bc are all intracellular bacteria, they are excellent targets for t cell-based vaccines. however, the sheer volume of available genomic data re ... | 2011 | 22130150 |
| thirty years of heme catalases structural biology. | about thirty years ago the crystal structures of the heme catalases from penicillium vitale (pvc) and, a few months later, from bovine liver (blc) were published. both enzymes were compact tetrameric molecules with subunits that, despite their size differences and the large phylogenetic separation between the two organisms, presented a striking structural similarity for about 460 residues. the high conservation, confirmed in all the subsequent structures determined, suggested a strong pressure t ... | 2011 | 22209752 |
| Bacterial cis-2-unsaturated fatty acids found in the cystic fibrosis airway modulate virulence and persistence of Pseudomonas aeruginosa. | There is an increasing appreciation of the polymicrobial nature of many bacterial infections such as those associated with cystic fibrosis (CF) and of the potentially important role for interspecies interactions in influencing both bacterial virulence and response to therapy. Patients with CF are often co-infected with Pseudomonas aeruginosa and other pathogens including Burkholderia cenocepacia and Stenotrophomonas maltophilia. These latter bacteria produce signal molecules of the diffusible si ... | 2011 | 22134647 |
| determination of the crystal structure and active residues of fabv, the enoyl-acp reductase from xanthomonas oryzae. | enoyl-acp reductase (enr) catalyses the last reduction reaction in the fatty acid elongation cycle in bacteria and is a good antimicrobial target candidate. fabv is the most recently discovered class of enr, but we lack information about the atomic structure and the key residues involved in reductase activity except for the known conserved tyrosine and lysine residues in the y-x(8)-k active site motif. | 2011 | 22039545 |
| antibiotic resistance and extended-spectrum β-lactamases in isolated bacteria from seawater of algiers beaches (algeria). | the aim of the study was to evaluate bacterial antibiotic resistance in seawater from four beaches in algiers. the most significant resistance rates were observed for amoxicillin and ticarcillin, whereas they were relatively low for ceftazidime, cefotaxime and imipenem. according to sampling sites, the highest resistance rates were recorded for 2 sites subjected to chemical and microbiological inputs (amoxicillin, 43% and 52%; ticarcillin, 19.6% and 47.7%), and for 2 sites relatively preserved f ... | 2011 | 22095134 |
| contamination of the cell sorter fluidics system with the water-borne bacterium burkholderia cepacia. | | 2011 | 21905213 |
| strategies for intracellular survival of burkholderia pseudomallei. | burkholderia pseudomallei is the causative agent of melioidosis, a disease with high mortality that is prevalent in tropical regions of the world. a key component of the pathogenesis of melioidosis is the ability of b. pseudomallei to enter, survive, and replicate within mammalian host cells. for non-phagocytic cells, bacterial adhesins have been identified both on the bacterial surface and associated with type 4 pili. cell invasion involves components of one or more of the three type 3 secretio ... | 2011 | 22007185 |
| copper as an antibacterial agent for human pathogenic multidrug resistant burkholderia cepacia complex bacteria. | the burkholderia cepacia complex (bcc) consists of 17 closely related multidrug resistant bacterial species that are difficult to eradicate. copper has recently gained attention as an antimicrobial agent because of its inhibitory effects on bacteria, yeast, and viruses. the objective of this study was to evaluate the antibacterial activity of copper surfaces and copper powder against members of the b. cepacia complex. the antibacterial activity of different copper surfaces was evaluated by incub ... | 2011 | 21925940 |
| An ensemble of structures of Burkholderia pseudomallei 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase. | Burkholderia pseudomallei is a soil-dwelling bacterium endemic to Southeast Asia and Northern Australia. Burkholderia is responsible for melioidosis, a serious infection of the skin. The enzyme 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (PGAM) catalyzes the interconversion of 3-phosphoglycerate and 2-phosphoglycerate, a key step in the glycolytic pathway. As such it is an extensively studied enzyme and X-ray crystal structures of PGAM enzymes from multiple species have been elucid ... | 2011 | 21904048 |