| the new quinolones and their combinations with other agents for therapy of severe infections. | new quinolones are very potent compounds against gram-negative bacteria, including pseudomonas, and staphylococci. they are particularly interesting because of their oral availability and their pharmacokinetic properties. when combined with other common antibiotics they appear to have mostly indifferent effects in vitro. clinical results have shown their efficacy when used alone for the treatment of osteomyelitis, complicated urinary tract infections and prostatitis. nevertheless, in 6% of cases ... | 1986 | 3011726 |
| transcription termination within the escherichia coli origin of dna replication, oric. | initiation of dna replication from the escherichia coli origin, oric, is dependent on an rna polymerase-mediated transcription event. the function of this rna synthetic event in initiation, however, remains obscure. since control of the synthesis of this rna could serve a key role in the overall initiation process, transcription regulatory sites within and near oric were identified using the galk fusion vector system. our results confirm the existence of a transcription termination signal within ... | 1986 | 3012276 |
| effect of doxycycline on oxygen-dependent killing mechanisms of human neutrophils. | the effects of doxycycline on neutrophil adhesivity, ingestion rate, and oxidative burst by particle and soluble compounds have been analyzed. the rate of bacterial ingestion by neutrophils as well as its subsequently particle-induced oxidative burst comprising oxygen uptake, hydrogen peroxide and superoxide anion productions, and iodination were all inversely correlated to doxycycline concentration included in the assay medium. the neutrophil oxidative burst induced by phorbol myristate (a solu ... | 1986 | 3013199 |
| in vitro binding of potent mutagenic pyrolysates to intestinal bacteria. | the ability of 22 strains of intestinal bacteria to bind the mutagenic pyrolyzates--3-amino-1,4-dimethyl-5h-pyrido-[4,3-b]indole [(trp-p-1) cas: 62450-06-0], 3-amino-1-methyl-5h-pyrido [4,3-b]indole [(trp-p-2) cas: 62450-07-1], 2-amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole [(glu-p-1) cas: 67730-11-4], 2-aminodipyrido[1,2-a:3',2'-d]imidazole [(glu-p-2) cas: 67730-10-3], 2-amino-3-methylimidazo[4,5-f]quinoline [(iq) cas: 76180-96-6], 2-amino-3,4-dimethylimidazo[4,5-f]quinoline [(meiq) cas: 7709 ... | 1986 | 3014197 |
| transfer of amikacin resistance by closely related plasmids in members of the family enterobacteriaceae isolated in chile. | during a 9-month period when amikacin was the sole aminoglycoside used clinically in a hospital in santiago, chile, resistance to amikacin and other antibiotics was encountered in 42 strains of the family enterobacteriaceae, including escherichia coli, klebsiella pneumoniae, citrobacter freundii, enterobacter cloacae, serratia marcescens, and serratia liquefaciens. amikacin resistance was transferable by conjugation and carried by incm plasmids ranging in size from ca. 48.4 to 58.1 kilobase pair ... | 1986 | 3015007 |
| potential prodrugs of 6-acetylmethylenepenicillanic acid (ro 15-1903). | the synthesis and biological activities of a series of non-classical penicillins are described. these compounds were synthesized by treating the pivaloyloxymethyl ester of 6-acetylmethylenepenicillanic acid (ro 15-1903) with various nucleophiles. they were found to be less active against the beta-lactamases from proteus vulgaris 1028, escherichia coli 1024, klebsiella pneumoniae nctc 418 and e. coli rtem than the parent compound. nevertheless, synergy with ampicillin against whole bacterial cell ... | 1986 | 3015853 |
| effects of acrolein on macrophage functions in rats. | male sprague-dawley rats were exposed to 0.1, 1.0 or 3.0 ppm acrolein or filtered air 6 h/day, 5 days/week for 3 weeks. rats were tested one day following the last exposure and exhibited no change in pulmonary clearance of inhaled 35s-labeled klebsiella pneumoniae at any acrolein concentration. decreased numbers of peritoneal cells were obtained from exposed rats while the number of cells lavaged from the lungs was unchanged. macrophages of acrolein-exposed rats had altered phagocytic and enzyma ... | 1986 | 3016949 |
| nifv-dependent, low-molecular-weight factor required for in vitro synthesis of iron-molybdenum cofactor of nitrogenase. | the molybdate- and atp-dependent in vitro synthesis of the iron-molybdenum cofactor of nitrogenase requires a low-molecular-weight factor. the factor is present in extracts of nitrogen fixation-derepressed cultures of klebsiella pneumoniae and azotobacter vinelandii, but not in extracts of repressed cultures of these bacteria. analysis of k. pneumoniae nif mutants has indicated that the nifv gene product is the only nif protein (besides nifa) necessary for the synthesis and accumulation of the f ... | 1986 | 3017921 |
| oral succession of gram-negative bacilli in myelosuppressed cancer patients. | aerobic and facultative gram-negative bacilli (gnb) have been reported to increase on various body surfaces in the seriously ill and debilitated patient. this study examined quantitative aspects of gnb succession at five oral sites in cancer patients before and during myelosuppressive chemotherapy. gnb concentrations increased sharply during chemotherapy at 25 to 50% of the oral sites in both acute nonlymphocytic leukemia and small-cell lung carcinoma patients. most sites did not exhibit shifts ... | 1986 | 3018036 |
| dissemination of a gentamicin resistance plasmid in the microbial population of hospital patients. | the dissemination of a gentamicin resistant plasmid, originally found in strains of klebsiella and termed pk181, into the microbial population of patients of the orvieto hospital was studied during 1982. five hundred and seventy-four strains of gram-negative bacilli were examined, transferable gentamicin resistance being revealed in five different bacterial species. the resistance was shown to be encoded by 81-megadalton plasmids in escherichia coli and enterobacter cloacae, and by 93-megadalton ... | 1986 | 3018453 |
| anaerobic regulation of nitrogen-fixation genes in rhodopseudomonas capsulata. | a rhodopseudomonas capsulata nifh::lacz gene fusion was used to isolate constitutive mutants of r. capsulata, unable to repress nif gene transcription anaerobically with every fixed-nitrogen source tested. when these nifc strains were grown aerobically, nif gene transcription was repressed. these results indicate that the regulation of nif gene transcription by fixed nitrogen is different from the regulation by oxygen. under anaerobic conditions, nif gene transcription in both r. capsulata and k ... | 1986 | 3018747 |
| synergy between ru 28965 (roxithromycin) and human neutrophils for bactericidal activity in vitro. | the in vitro effects of ru 28965 (roxithromycin), a new semisynthetic macrolide, on human neutrophil activity were compared with those of erythromycin. ru 28965, at a concentration as low as 0.1 microgram/ml, significantly enhanced the phagocytosis and killing of staphylococcus aureus by neutrophils. erythromycin displayed a less stimulating effect in a dose-dependent manner. phagocytosis of klebsiella pneumoniae was also increased after incubation of neutrophils with ru 28965, but killing was n ... | 1986 | 3019233 |
| two-component regulatory systems responsive to environmental stimuli share strongly conserved domains with the nitrogen assimilation regulatory genes ntrb and ntrc. | we report that the ntrb and ntrc proteins of bradyrhizobium sp. [parasponia] strain rp501 share homology with other regulatory proteins. there is extensive conservation of c-terminal regions between products of rp501 ntrb; klebsiella pneumoniae ntrb; escherichia coli envz, cpxa, and phor; agrobacterium tumefaciens vira; and, to a lesser extent, e. coli chea. there is also extensive conservation of n-terminal regions between products of rp501 ntrc; k. pneumoniae ntrc; e. coli ompr, sfra, phob, ch ... | 1986 | 3020561 |
| paediatric meningitis in the western cape. a 3-year hospital-based prospective survey. | between july 1981 and june 1984 1223 cases of meningitis were seen in the department of paediatrics, tygerberg hospital. the commonest form in each population group was aseptic meningitis. positive viral cultures were obtained from the csf in 108 cases. the median age of white children with aseptic meningitis, 64 months, was significantly greater than that of coloured children, 45 months (p greater than 0.0001), and black children, 26 months (p greater than 0.014). the commonest cause of confirm ... | 1986 | 3020718 |
| molecular cloning of amikacin resistance determinants from a klebsiella pneumoniae plasmid. | a multiresistant klebsiella pneumoniae strain, jhck1, harbored several plasmids. one of these, plasmid pjhcmw1, carried determinants for resistance to amikacin in addition to kanamycin, tobramycin, and ampicillin. the amikacin resistance determinant(s) was cloned and studied by restriction mapping, insertion, and deletion analysis. the amikacin resistance gene(s) was localized in a 1.5-kilobase dna fragment. this pjhcmw1 dna region was responsible for not only the resistance to amikacin but also ... | 1986 | 3021052 |
| plasmid-specified aminoglycoside-modifying enzymes in clinical isolates of klebsiella pneumoniae. | in 106 clinical isolates of multiresistant klebsiella pneumoniae strains, we found that aminoglycoside-resistance was due mostly to two adenylating enzymes: aad (2") (56.6%), that modifies gentamicin, kanamycin, tobramycin and sissomicin, and aad (3") 9 (56.6% + 19.8%) that modifies streptomycin and spectinomycin. the identification of these enzymes was possible by mics determination against a set of aminoglycosides antibiotics. aad (2") + aad (3")9 were coded by conjugative plasmid of about 120 ... | 1985 | 3021514 |
| restriction enzyme fingerprinting of enterobacterial plasmids: a simple strategy with wide application. | restriction enzyme fingerprints were generated from purified plasmid dna from 324 clinical isolates that belonged to 7 enterobacterial genera and 88 single plasmids in escherichia coli k 12 according to the following strategy. purified plasmid dna was digested with psti. the number of fragments detected in a 0.8 agarose gel was used to determine which 2 of 6 restriction enzymes including psti was most likely to provide a fingerprint comprising sufficient fragments to ensure specificity but suffi ... | 1986 | 3023479 |
| modification of susceptibility to klebsiella pneumoniae during murine cytomegalovirus infection. | the effect of murine cytomegalovirus (mcmv) infection on susceptibility to bacterial infection was studied in mice by a combination of intraperitoneal (ip) inoculation of a sublethal dose of mcmv with subsequent ip challenge of 2 x 10(3) cfu of a strain of klebsiella pneumoniae (kp). when given alone, kp produced a mortality of 30-40%. mortality was increased when kp was given 1 to 7 days after mcmv injection with the peak increase at the 4th to 5th day when 100% mortality occurred. virus levels ... | 1986 | 3023797 |
| infections in patients with non-small-cell lung cancer treated with intensive induction chemotherapy. | the records of 65 consecutive patients with non-small-cell lung cancer (nsclc) treated with intensive induction chemotherapy were reviewed to study the infectious complications during therapy and to analyze the relationship of the frequency of infections to various predisposing factors. a total of 44 infectious episodes were observed among 30 of the 65 patients. of the 44 infections, 18 were microbiologically documented and 19 clinically. seven (16%) infections were without microbiological or cl ... | 1986 | 3023801 |
| complementation of nitrogen-regulatory (ntr-like) mutations in rhodobacter capsulatus by an escherichia coli gene: cloning and sequencing of the gene and characterization of the gene product. | in vivo genetic engineering by r' plasmid formation was used to isolate an escherichia coli gene that restored the ntr+ phenotype to ntr- mutants of the photosynthetic bacterium rhodobacter capsulatus (formerly rhodopseudomonas capsulata; j. f. imhoff, h. g. trüper, and n. pfenning, int. j. syst. bacteriol. 34:340-343, 1984). nucleotide sequencing of the gene revealed no homology to the ntr genes of klebsiella pneumoniae. furthermore, hybridization experiments between the cloned gene and differe ... | 1987 | 3025172 |
| sulbactam: biochemical factors involved in its synergy with ampicillin. | sulbactam is a time-dependent irreversible inhibitor of various beta-lactamases by reversible formation of a michaelis-type enzyme-inhibitor complex and progressive evolution of this complex into inactivated protein(s). this process is either irreversible (true inactivation) or quasi-irreversible (stable acyl-enzyme). in this way, sulbactam efficiently protects ampicillin from degradation by beta-lactamases. sulbactam itself exhibits a moderate antibacterial activity that is related to an affini ... | 1986 | 3025996 |
| sulbactam/ampicillin: in vitro spectrum, potency, and activity in models of acute infection. | more than 90% of community hospital-isolated strains of staphylococcus (including methicillin-resistant isolates), streptococcus, haemophilus, neisseria, branhamella, bacteroides, escherichia coli, klebsiella, enterobacter aerogenes, proteus, and acinetobacter calcoaceticus were inhibited by the sulbactam/ampicillin (1:2) combination at concentrations of 8 micrograms/16 micrograms per ml. the peak serum level from a 15-min infusion of 1 g/2 g of sulbactam/ampicillin is more than seven times this ... | 1986 | 3026001 |
| activity of sulbactam/ampicillin in screening and discriminative animal models of infection. | the efficacy of sulbactam/ampicillin in the treatment of mice with fatal systemic infections produced by ampicillin-resistant staphylococcus aureus, haemophilus influenzae, klebsiella pneumoniae, or proteus vulgaris strains is well established. in this paper the demonstrations of efficacy for sulbactam/ampicillin have been extended to a number of clinically relevant models, including bacteremia and meningitis produced by h. influenzae in infant rats, experimental staphylococcal endocarditis in r ... | 1986 | 3026002 |
| sulbactam/ampicillin vs. chloramphenicol/ampicillin for the treatment of meningitis in infants and children. | eighty-one patients ages one month to 14 years with meningitis were randomized to receive either sulbactam (50 mg/kg per day) and ampicillin (400 mg/kg per day; 41 patients) or chloramphenicol and ampicillin (40 patients). the groups were comparable in terms of sex and degree of illness; however, more patients treated with chloramphenicol/ampicillin than patients treated with sulbactam/ampicillin were younger than 12 months of age (78% vs. 56%). pathogens were isolated from the cerebrospinal flu ... | 1986 | 3026015 |
| clinical efficacy of sulbactam/ampicillin in pediatric infections caused by ampicillin-resistant or penicillin-resistant organisms. | twenty-three children two months to 11 years old were treated with sulbactam/ampicillin or sulbactam/penicillin. eleven had urinary tract infections (uti), eight had pus-forming cervical adenitis, and four had lobar pneumonia. pathogens were isolated from 18 patients: escherichia coli from 10, staphylococcus aureus from seven, and klebsiella pneumoniae from one. all isolates were resistant to ampicillin or penicillin alone. sulbactam (50 mg/kg per day) plus ampicillin (1:2 or 1:3 ratio) or penic ... | 1986 | 3026016 |
| [cytomegalovirus-associated superinfection of the lung following kidney transplantation]. | in a kidney-transplant patient, there was superinfection of the lungs by klebsiella pneumoniae and legionella pneumophilia with multiple necroses in the course of a primary cytomegalovirus infection. in a later phase of the disease, there was an opportunistic colonization of the necrotic cavities and the adjacent lung tissue by aspergillus fumigatus and candida albicans. the cytomegalovirus infection led to a pronounced cellular immunosuppression in the patient. this favored superinfections by b ... | 1987 | 3026768 |
| deletion loop mutagenesis of the nifl promoter from klebsiella pneumoniae: role of the -26 to -12 region in promoter function. | nine single c-to-t transitions were introduced into the -26 to -12 region of the klebsiella pneumoniae nifl promoter by bisulphite mutagenesis of m13 heteroduplexes containing a 15 nucleotide single-stranded loop. mutant promoter fragments were inserted into translational lac fusion vectors to utilise beta-galactosidase activity as a measure of promoter efficiency. mutations in invariant nucleotides found in the consensus sequence for nif promoters gave a strong 'down' promoter phenotype with re ... | 1986 | 3026913 |
| nucleotide sequence of the nifla operon of klebsiella oxytoca ng13 and characterization of the gene products. | the complete nucleotide sequence of the regulatory operon nifla of a nitrogen fixer klebsiella oxytoca ng13 was determined, and the transcriptional start point was assigned by s1 mapping. the nifl protein (a repressor) was coded by an open reading frame of 1,485 bases, corresponding to a protein of 495 amino acids with a calculated molecular weight of 55,242. the open reading frame (1,572 bases) of the nifa protein (an activator), corresponding to a molecular weight of 58,649, was confirmed by i ... | 1986 | 3027503 |
| protective effect of nocardia rubra cell wall skeleton on experimental infection in normal and immunosuppressed mice. | the protective effect of nocardia rubra cell wall skeleton (n-cws) on experimental infections was investigated in normal and immunosuppressed mice. pretreatment with n-cws provided protection against acute systemic infections due to pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, serratia marcescens and listeria monocytogenes in normal mice. n-cws administered before or after challenge also showed protective activity against herpes simplex virus infection in normal mice. n-cws w ... | 1986 | 3028434 |
| beta-lactamase inhibitors: another approach to overcoming antimicrobial resistance. | beta-lactamase inhibitors in combination with a beta-lactam antimicrobial agent provide another approach to the treatment of infections caused by many bacteria resistant to commonly used agents. currently two fixed combinations are commercially available. one is the oral preparation of amoxicillin and potassium clavulanate (augmentin). the other is for intravenous therapy and combines ticarcillin and potassium clavulanate (timentin). in vitro studies confirm that the combination of a beta-lactam ... | 1987 | 3028975 |
| conservation of structure and location of rhizobium meliloti and klebsiella pneumoniae nifb genes. | using transposon tn5-mediated mutagenesis, an essential rhizobium meliloti nitrogen fixation (nif) gene was identified and located directly downstream of the regulatory gene nifa. maxicell and dna sequence analysis demonstrated that the new gene is transcribed in the same direction as nifa and codes for a 54-kilodalton protein. in klebsiella pneumoniae, the nifbq operon is located directly downstream of a gene which is structurally and functionally homologous to the r. meliloti nifa gene. the dn ... | 1987 | 3029020 |
| genetic and structural analysis of the rhizobium meliloti fixa, fixb, fixc, and fixx genes. | the fixa, fixb, fixc, and fixx genes of rhizobium meliloti 1021 constitute an operon and are required for nitrogen fixation in alfalfa nodules. dna homologous to the r. meliloti fixabc genes is present in all other rhizobium and bradyrhizobium species examined, but fixabc-homologous sequences were found in only one free-living diazotroph, azotobacter vinelandii. to determine whether the fixabcx genes share sequence homology with any of the 17 klebsiella pneumoniae nif genes, we determined the en ... | 1987 | 3029021 |
| cloning and expression in escherichia coli of a klebsiella ozaenae plasmid-borne gene encoding a nitrilase specific for the herbicide bromoxynil. | an enzyme (nitrilase) that converts the herbicide bromoxynil (3,5-dibromo-4-hydroxybenzonitrile) to its metabolite 3,5-dibromo-4-hydroxybenzoic acid was shown to be plasmid encoded in the natural soil isolate klebsiella ozaenae. the bromoxynil-specific nitrilase was expressed in escherichia coli by direct transfer and stable maintenance in e. coli of a naturally occurring 82-kilobase k. ozaenae plasmid. irreversible loss of the ability to metabolize bromoxynil both in e. coli and k. ozaenae was ... | 1987 | 3029038 |
| organization and partial sequence of a dna region of the rhizobium leguminosarum symbiotic plasmid prl6ji containing the genes fixabc, nifa, nifb and a novel open reading frame. | by hybridization and heteroduplex studies the fixabc and nifa genes of the rhizobium leguminosarum symbiotic plasmid prl6ji have been identified. dna sequencing of the region containing nifa showed an open reading frame of 1557 bp encoding a protein of 56, 178 d. based on sequence homology, this orf was confirmed to correspond to the nifa gene. comparison of three nifa proteins (klebsiella pneumoniae, rhizobium meliloti, rhizobium leguminosarum) revealed only a weak relationship in their n-termi ... | 1987 | 3029674 |
| antibiotic use as an inducer of resistant urinary tract infections. | urinary tract infection is second in frequency only to respiratory infection as an indication for antimicrobial chemotherapy. as a result, a significant proportion of both domiciliary and hospital patient populations are exposed to chemotherapy which, in turn, may act as a potent selection pressure for development of resistance mediated by intrinsic mechanisms or acquisition of r-plasmids or transposons. the widespread use of broad spectrum agents has encouraged the development of such resistanc ... | 1986 | 3029858 |
| a comparative study on the phoe genes of three enterobacterial species. implications for structure-function relationships in a pore-forming protein of the outer membrane. | the cloned phoe genes from enterobacter cloacae and klebsiella pneumoniae are normally expressed and regulated in escherichia coli k-12, and their products are correctly assembled into the outer membrane. differences between the three phoe proteins were found with binding of two out of ten monoclonal antibodies directed against the cell-surface-exposed part and in pore characteristics, but not in phage receptor function. the dna sequences of the e. cloacae and k. pneumoniae phoe genes were deter ... | 1987 | 3032618 |
| cefodizime (hr 221) potentiation of human neutrophil oxygen-independent bactericidal activity. | the enhanced bactericidal activity of human neutrophils induced by cefotaxime and cefodizime, two methoxy-imino-amino- 2-thiazolyl cephalosporins, is linked to the cell stimulation of oxygen-dependent and oxygen-independent killing systems, respectively. cefotaxime enhances both the killing and the oxidative response of neutrophils to opsonized particulate stimuli (bacteria for both activities and opsonized zymosan for the oxidative burst). these effects were not observed with non-opsonized part ... | 1987 | 3032885 |
| construction of a transposon containing a gene for polygalacturonate trans-eliminase from klebsiella oxytoca. | a dna fragment containing a klebsiella oxytoca gene for polygalacturonate trans-eliminase was cloned into the kanamycin resistance transposon tn5. this new transposon, designated tn5-pga+, had a transposition frequency of 1 x 10(-6). the broad host range plasmid pr751::tn5-pga+ was conjugally transferred to a variety of genetic backgrounds. the ability to degrade polygalacturonate was expressed in aeromonas hydrophila, alcaligenes eutrophus, azotomonas insolita, escherichia coli, pseudomonas put ... | 1987 | 3034186 |
| rhizobium meliloti ntra (rpon) gene is required for diverse metabolic functions. | we report the identification and cloning of an ntra-like (glnf rpon) gene of rhizobium meliloti and show that the r. meliloti ntra product (ntra) is required for c4-dicarboxylate transport as well as for nitrate assimilation and symbiotic nitrogen fixation. dna sequence analysis showed that r. meliloti ntra is 38% homologous with klebsiella pneumoniae ntra. subcloning and complementation analysis suggested that the r. meliloti ntra promoter lies within 125 base pairs of the initiation codon and ... | 1987 | 3034856 |
| in-vitro anti-microbial susceptibility of clinical isolates of pathogenic bacteria to ten antibiotics including phosphomycin. | the minimum inhibitory concentration (mic) of ten antibiotics was determined for various bacterial pathogens isolated from clinical specimens in a lagos hospital. the in-vitro activity of penicillin and tetracycline was not very impressive and a similarity was noticed in the resistance patterns of these two antibiotics, while the activity in vitro of the relatively more toxic aminoglycosides and chloramphenicol was high for gram-negative rods. ceftazidime demonstrated the highest activity in vit ... | 1987 | 3037870 |
| in vitro antimicrobial activity of diethyldithiocarbamate and dimethyldithiocarbamate against methicillin-resistant staphylococcus. | staphylococcus aureus has appeared which is highly resistant to both methicillin and aminoglycosides. current therapy involves long-term intravenous therapy of vancomycin. since vancomycin is currently the only drug used to treat these patients, there is a need to develop additional antimicrobial therapy. the in vitro antimicrobial effect of the metal chelator, diethyldithiocarbamate (ddtc) and its structural analog dimethyldithiocarbamate (dmtc) were investigated. both ddtc and dmtc were effect ... | 1987 | 3037985 |
| monocyclic and tricyclic analogs of quinolones: mechanism of action. | the mode of action of ro 13-5478 and ro 14-9578, monocyclic and tricyclic quinolone analogs, respectively, was examined for escherichia coli and staphylococcus aureus. the compounds showed antibacterial activity and effects on cell morphology, replicative dna biosynthesis, and gyrase-catalyzed dna supercoiling that were comparable to those shown by nalidixic acid and by oxolinic acid compounds. the results suggest that their site of action is dna gyrase and that a bicyclic quinolone nucleus is n ... | 1987 | 3037999 |
| [sulbactam and clavulanic acid: studies of enzyme kinetics and synergism with ampicillin and mezlocillin]. | both inhibitors clavulanic acid and sulbactam exhibit high affinity (ki-values less than 10(-6) mol/l) to the beta-lactamases of gram-negative bacteria with predominant penicillinase activity and lowered affinity to enzymes with cephalosporinase activity (above all clavulanic acid). as compared to sulbactam there was a stronger synergism of clavulanic acid with ampicillin or mezlocillin in those isolates producing either a plasmid-mediated or a chromosomally-mediated penicillinase. in beta-lacta ... | 1987 | 3038733 |
| clostridial pyruvate oxidoreductase and the pyruvate-oxidizing enzyme specific to nitrogen fixation in klebsiella pneumoniae are similar enzymes. | the chemical characterization, epr properties, and mechanism of pyruvate:flavodoxin (ferredoxin) oxidoreductase from klebsiella pneumoniae and clostridium thermoaceticum have been investigated. a simple, specific, and sensitive assay and an efficient purification (based on the high affinity of these enzymes for a dye attached to agarose) are reported. the observed iron content of 8 atoms/subunit is twice that reported by others, whereas the contents of lipoate and flavin are less than 0.1 mol/su ... | 1987 | 3038882 |
| effects exerted by ru 41740 on oxidative metabolism and migration of rat polymorphonuclear leukocytes collected after induction of one acute non specific inflammatory reaction. | the activity of ru 41740, a glycoprotein extract from klebsiella pneumoniae, endowed with immuno-modulating properties, has been investigated on polymorphonuclear (pmn) leukocyte functions. this report deals with the effect of ru 41740 on oxidative metabolism (assessed by chemiluminescence, 02 consumption and o2- production) and on chemotaxis and random migration (using agarose and boyden chamber techniques). pmns were collected from the rat pleural cavity after induction of one acute inflammato ... | 1987 | 3040607 |
| comparative organization of nitrogen fixation-specific genes from azotobacter vinelandii and klebsiella pneumoniae: dna sequence of the nifusv genes. | in the facultative anaerobe klebsiella pneumoniae 17 nitrogen fixation-specific genes (nif genes) have been identified. homologs to 12 of these genes have now been isolated from the aerobic diazotroph azotobacter vinelandii. comparative studies have indicated that these diverse microorganisms share striking similarities in the genetic organization of their nif genes and in the primary structure of their individual nif gene products. in this study the complete nucleotide sequence of the nifusv ge ... | 1987 | 3040672 |
| transcription of a rhizobium leguminosarum biovar phaseoli gene needed for melanin synthesis is activated by nifa of rhizobium and klebsiella pneumoniae. | the rhizobium leguminosarum biovar phaseoli symbiotic plasmid prp2ji carries a gene, mela, specifying the enzyme tyrosinase, which is responsible for the production of the pigment melanin in these bacteria. transcription of mela is activated by the nifa gene of rhizobium and, when the cloned mela gene is transferred to escherichia coli, mela is expressed if the recipients contain nifa gene of klebsiella pneumoniae. this nifa-dependent activation was temperature sensitive and required the ntra ge ... | 1988 | 3041240 |
| experimental otitis media with effusion induced by lipopolysaccharide from klebsiella pneumoniae: mucociliary pathology of the middle ear. | we inoculated 100 micrograms/ml of lipopolysaccharide (lps) from klebsiella pneumoniae into the tympanic cavity of guinea pigs and examined the mucociliary pathology in the middle ear. serous effusion was observed in the tympanic cavity of every animal on the first, third, and seventh day following the procedure, but the volume of the effusion had decreased to 0.2 ml on day 7. by that time, the ciliary activity in the opening to the eustachian tube within the middle ear had recovered to some ext ... | 1988 | 3041864 |
| lymphocyte response to enterobacterial biostructures in seronegative spondarthritis: specific t-cell mediated immunity or non-specific polyclonal b-cell activation? | the proliferative response, as measured by thymidine uptake and the b-cell differentiation response, as measured by ig secretion, have been studied in peripheral blood mononuclear cells obtained from patients with ankylosing spondylitis, yersinia reactive arthritis, klebsiella infections and healthy controls, following stimulation with enterobacterial biostructures. | 1988 | 3042075 |
| molecular mimicry: fact or fiction? | molecular studies suggest that peptide sequence similarities are present between hla-b27 and bacteria, but the pathological mechanisms in spondyloarthropathies remain unclear. | 1988 | 3042076 |
| klebsiella antibodies in ankylosing spondylitis and proteus antibodies in rheumatoid arthritis. | antibodies to klebsiella, but not to other bacteria, have been shown to be present in patients with active ankylosing spondylitis (as) by seven different techniques. antibodies to proteus, but not to e. coli or klebsiella, have been shown to be present in patients with active rheumatoid arthritis (ra) by three different techniques. it is suggested as and ra are forms of reactive arthritis, to klebsiella and proteus respectively, probably mediated by cross-reactivity to hla antigens. | 1988 | 3042077 |
| antibodies to klebsiella and proteus microorganisms in ankylosing spondylitis and rheumatoid arthritis patients measured by elisa. | antibodies to klebsiella oxytoca and proteus mirabilis in 21 active ankylosing spondylitis (as), 13 active rheumatoid arthritis (ra), 19 inactive ra patients and 18 healthy controls were measured using enzyme-linked immunosorbent assay (elisa). elevated anti-klebsiella antibodies were demonstrated in active as patients compared to active ra (p less than 0.01), inactive ra patients (p less than 0.001) and controls (p less than 0.005). when measuring antibodies to proteus, active ra patients showe ... | 1988 | 3042078 |
| dissociation of the sodium-ion-translocating oxaloacetate decarboxylase of klebsiella pneumoniae and reconstitution of the active complex from the isolated subunits. | oxaloacetate decarboxylase was reconstituted from the purified alpha subunit and a triton x-100 extract of bacterial membranes devoid of this protein. upon freezing of oxaloacetate decarboxylase in salt solutions, the enzyme was split into subunits and the catalytic activity was abolished. the catalytically active decarboxylase complex was reconstituted by decreasing the salt concentration of the dissociated sample. the conditions for the inactivation were critical for an optimum recovery of cat ... | 1988 | 3042395 |
| immunization with rough mutants of salmonella minnesota: protective activity of igm and igg antibody to the r595 (re chemotype) mutant. | we evaluated the immunoglobulin class responsible for the protective activity in serum obtained from humans and rabbits after immunization with the r595 (re chemotype) mutant of salmonella minnesota. whole serum obtained before immunization and the igg and igm fractions failed to protect mice against lethal challenge with viable klebsiella pneumoniae or morganella morganii or with salmonella typhi lipopolysaccharide (lps). the protective activity of postimmunization serum resided solely in igm a ... | 1988 | 3042871 |
| immunization with rough mutants of salmonella minnesota: initial studies in human subjects. | vaccines prepared from unheated and boiled, acetone-precipitated salmonella minnesota r595 (re chemotype mutant) were administered subcutaneously to 122 healthy volunteers. titers of antibody to re lipopolysaccharide, the basal core structure of endotoxin, as measured by indirect hemagglutination, rose in a dose-responsive fashion after immunization. febrile reactions, usually mild, occurred after 7% of injections with the highest doses (2.0 and 3.0 x 10(10) organisms), and mild local soreness a ... | 1988 | 3042872 |
| enhancement of growth of aerobic, anaerobic, and facultative bacteria in mixed infections with anaerobic and facultative gram-positive cocci. | the potential for mutual enhancement of growth of seven strains of anaerobic and facultative gram-positive cocci (afgpc), seven aerobic and facultative organisms, and two bacteroides spp. commonly isolated with afgpc in mixed infection was evaluated. enhancement was studied by measuring the relative increase in the colony-forming units of the two bacterial components inducing subcutaneous abscesses in mice. of the 56 combinations, afgpc were enhanced in 6 and inhibited in 1. the aerobic and facu ... | 1988 | 3043109 |
| [in vitro bactericidal effect of cefotetan-aminoside combinations]. | bactericidal activity of cefotetan-gentamicin combinations was studied on 6 bacterial strains: s. aureus, k. pneumoniae, e. cloacae, s. marcescens, p. vulgaris, p. stuarti. time kille curves technic was performed with final concentrations of cefotetan: 4 at 32 mg/l, and of gentamicin: 0.25; 0.5; 1; 2; 8 mg/l and with an 10(6) cfu/ml inoculum. cefotetan at 4 mg/l was not able to obtain a 0.01% (percentage of survivors) bactericidal activity before 24 h. the combination cefotetan-gentamicin (0.25 ... | 1988 | 3043329 |
| [kinetics of ofloxacin-amoxicillin and ofloxacin-clavulanic acid combinations against pathogenic bacteria of the respiratory tract]. | ofloxacin exhibit a good activity against the pathogen bacteria of the respiratory tract, except for s. pneumoniae. its use is interesting because it has a good diffusion into respiratory tissues. however, the combination with an another antibiotic was necessary to spread the activity spectrum and to prevent the resistant variants. by the new killing curve checkerboard method, these 2 combinations are studied against 8 strains: 4 h. influenzae (2 beta-lactamase producers), 2 s. pneumoniae and 2 ... | 1988 | 3043337 |
| [activity of 9 beta-lactam antibiotics combined with clavulanic acid or sulbactam against the strains of broad-spectrum beta-lactamase (ctx-1) producing enterobacteriaceae isolated at the henri mondor hospital]. | minimal inhibitory concentrations (mics) of seven cephalosporins: cefotaxime (ctx), ceftriaxone (cro), ceftazidime (caz), latamoxef (mox), cefoxitin (fox), cefotetan (ctt) and cm 40876 (cm), of aztreonam (atm) and imipenem (ipm) were evaluated by agar dilution with and without 5 mg/l of clavulanic acid (ac) or sulbactam (sb) for 28 strains isolated in 1986 (15 k. pneumoniae, 3. k. oxytoca, 4 e. coli, 4 e. cloacae, 1 e. aerogenes and 1 c. freundii). comparatively to mics of sensitive strains and ... | 1988 | 3043338 |
| [current status of aminoglycoside resistance in hospital enterobacteriaceae]. | we studied the susceptibility of enterobacteriaceae to four aminoglycosides (gentamicin, tobramycin, netilmicin et amikacin). we determined their phenotypes of resistance by taking into account both the critic concentrations of the cfa (french committee for antibiogram) and the mic of the main susceptible population of each species. the most frequent phenotypes were gtnt, tnta and gtnta. amikacin resistance including phenotypes were essentially found in klebsiella and serratia (35% and 53% of th ... | 1988 | 3043339 |
| [use of imipenem-cilastatin in neonatal septicemias caused by gram-negative bacilli multiresistant to beta-lactam antibiotics]. | seven neonates with septicemia due to gram negative bacteria resistant to beta-lactam received imipenem-cilastatin therapy. bacteria isolated were enterobacter cloacae [3], enterobacter aerogenes [1], klebsiella pneumoniae [1], serratia marcescens [1], pseudomonas fluorescens [1]. the mics of imipenem were lower 1 microgram/ml. in 3 children septicemia occurred during previous antimicrobial chemotherapy. 3 iv 60 mg/kg doses of imipenem with amikacin (15 mg kg/d) were administered every day. for ... | 1988 | 3043351 |
| bartholin's gland abscess in infancy. | | 1988 | 3043366 |
| nucleotide and deduced amino acid sequences of the klebsiella pneumoniae nifk gene coding for the beta-subunit of nitrogenase mofe protein. | | 1988 | 3043382 |
| functional disorder of eustachian tube in experimental otitis media with effusion following inoculation of bacterial endotoxin. | a 10-micrograms/ml solution of lipopolysaccharide derived from klebsiella pneumoniae was inoculated into the middle ears of guinea pigs. the animals were killed painlessly on the first, third, or seventh day after inoculation, and the mucosal samples from the bony portion of the eustachian tube were examined for ciliary activity and epithelial morphology. on the first and third days, when middle ear effusions were present, deterioration of ciliary activity and morphologic changes in the mucocili ... | 1988 | 3044230 |
| dinitrogenase with altered substrate specificity results from the use of homocitrate analogues for in vitro synthesis of the iron-molybdenum cofactor. | the in vitro synthesis of the iron-molybdenum cofactor (femo-co) of nitrogenase requires homocitrate (2-hydroxy-1,2,4-butanetricarboxylic acid). homocitrate is apparently synthesized by the nifv gene product. in the absence of homocitrate, no femo-co is formed in vitro, as determined from coupled c2h2 reduction assays and the lack of 99mo label incorporation into apodinitrogenase. several organic acids were tested for their ability to replace homocitrate in the femo-co synthesis system. with app ... | 1988 | 3044446 |
| unconjugated bilirubin in hepatic bile with brown pigment gallstones and cholangitis. | to investigate the role of cholangitis in hydrolysis of bilirubin in bile with brown pigment gallstones, bilirubin composition and bacterial growth in hepatic bile with and without cholangitis were studied. the study included 38 brown pigment gallstone cases (28 without cholangitis and 10 with cholangitis). the proportion of unconjugated bilirubin in hepatic bile with cholangitis (16.9 +/- 8.5%, mean +/- sd) was significantly higher than that without cholangitis (3.7 +/- 1.8%, p less than 0.001) ... | 1988 | 3044714 |
| application of a microcomputer-based system in the analysis of infection data at the emergency units of a large hospital. | after three years of retrospective study in four emergency units from a large hospital (2000 beds) and analysis of 6283 positive cultures, a microcomputer database system was built to store information concerning nosocomial infections in order to help the clinical staff from those units to study the incidence of 20 bacterial species and their sensitivity pattern evolutions for 27 antibiotics (from samples in 15 different collecting sites). this system was developed as an alternative to the hospi ... | 1988 | 3045020 |
| ceftazidime combined with mecillinam: serum bactericidal titres compared with in-vitro synergy against gram-negative bacilli. | in a study of the possible interaction between mecillinam and ceftazidime against gram-negative bacilli, ten volunteers received on separate days: ceftazidime 20 mg/kg iv in 15 min, mecillinam 10 mg/kg iv in 15 min, or the combination. blood samples were obtained before and 1 and 6 h after the end of the infusion. ten strains each of klebsiella pneumoniae, serratia marcescens, citrobacter freundii, salmonella spp. and yersinia spp. and nine strains each of acinetobacter spp., and pseudomonas aer ... | 1988 | 3045067 |
| klebsiella pneumoniae as a cause of pneumonia and septicemia in a civet kitten (civettictis civetta) in the jos zoo, nigeria. | a fatal case of acute pneumonia and septicemia that occurred in a captive civet kitten (civettictis civetta) in the jos zoo, nigeria is reported. diagnosis was based on clinical signs, necropsy findings, and the isolation of klebsiella pneumoniae from the lung, intestine, liver and heart blood of the animal. this is the first report of clinical k. pneumoniae infection in a wild or captive animal in nigeria. | 1988 | 3045348 |
| iron k-edge x-ray absorption spectroscopy of the iron-molybdenum cofactor of nitrogenase from klebsiella pneumoniae. | iron k-edge x-ray absorption data for the iron-molybdenum cofactor ('femoco') from klebsiella pneumoniae reported here provide the first evidence for long-range structural order in the cofactor [fe...fe(mo) = 0.368 nm in addition to fe...s = 0.22 nm and fe...fe(mo) = 0.27 nm] and, in contrast with previously published data [antonio, teo, orme-johnson, nelson, groh, lindahl, kauzlarich & averill (1982) j. am. chem. soc. 104, 4703-4705], indicate that most of the iron centres are not co-ordinated ... | 1988 | 3046607 |
| acute rheumatoid factor positive (igm) polyarthritis associated with a klebsiella pneumonitis. | the authors report a case of a patient suffering from acute polyarthritis with a high rheumatoid factor titre, associated with a klebsiella pneumonitis. a polyclonal b lymphocyte activation or a possible cross reaction between rheumatoid factor and an antigen related to klebsiella may explain the elevated production of rheumatoid factor observed. | 1988 | 3046829 |
| sulbactam/ampicillin, a new beta-lactamase inhibitor/beta-lactam antibiotic combination. | sulbactam/ampicillin is a combination of a beta-lactamase inhibitor with minimal intrinsic antibacterial activity (sulbactam sodium), and an aminopenicillin (ampicillin sodium). the addition of sulbactam to ampicillin has no effect on the chemical stability of ampicillin in aqueous solution, and the administration guidelines of the combination are the same as for ampicillin alone. sulbactam acts primarily by irreversible inactivation of beta-lactamases from most beta-lactamase-producing organism ... | 1988 | 3046887 |
| chronic bacterial prostatitis: 10 years of experience with local antibiotics. | direct transperineal infiltration of the prostate with antibiotics was performed in 24 selected patients with refractory chronic bacterial prostatitis. diagnosis had been made following a modification of the classical lower tract localization studies. remission periods of at least 6 months were obtained in 71 per cent of these patients after 1 or 2 infiltrations, while 6 of 24 (25 per cent) required several procedures to assume a long-term remission state and 1 (4 per cent) failed therapy (patie ... | 1988 | 3047435 |
| protection against fatal klebsiella pneumoniae sepsis in the squirrel monkey saimiri sciureus after immunization with a capsular polysaccharide vaccine. | an anti-klebsiella pneumoniae k5a capsular polysaccharide vaccine was evaluated as a preventive approach for protecting squirrel monkeys, saimiri sciureus, in our breeding colony. based on an 8-month vaccination schedule over a period of more than two years, this vaccine, regardless of the animal's age, resulted in a reduction of this particular bacterial infection and its generally associated fatal outcome. igg antibody responses in naive and vaccinated animals were monitored over an extended p ... | 1988 | 3048318 |
| the herbicidally active experimental compound hoe 704 is a potent inhibitor of the enzyme acetolactate reductoisomerase. | growth inhibition of plants and bacteria by the experimental herbicide hoe 704 (2-methylphosphinoyl-2-hydroxyacetic acid) was alleviated by the addition of the branched-chain amino acids to growth media. hoe 704 caused a massive accumulation of acetoin and acetolactate, indicating its direct interference with the branched-chain amino acid biosynthetic pathway. the second enzyme of this pathway, acetolactate reductoisomerase (ec 1.1.1.86), was found to be subject to strong inhibition by hoe 704. ... | 1988 | 3049163 |
| inactivation of bacteria by purogene. | the bacteriocidal efficacy of purogene, a stabilized aqueous solution of chlorine dioxide (clo2) was examined using bacteria of concern to public health. the organisms tested were: escherichia coli, pseudomonas aeruginosa, yersinia enterocolitica, klebsiella pneumoniae, streptococcus pyogenes group a, salmonella typhimurium and bacillus subtilis. the test organisms responded differently to inactivation by purogene. at least a 4 log reduction in bacterial counts was noted when purogene was applie ... | 1988 | 3049506 |
| starvation and nutrient resuscitation of klebsiella pneumoniae isolated from oil well waters. | klebsiella pneumoniae isolated from oil well waters reduced in size in response to nutrient starvation. the cells remained viable during starvation and later were able to grow rapidly when stimulated by nutrients. the heterotrophic potential, culture absorbance and extracellular polysaccharide production decreased during cell starvation whereas an initial increase in colony-forming units was observed on agar plates. transmission electron microscopy (tem) after 24 d revealed that the cells had ch ... | 1988 | 3049508 |
| novel plasmid-mediated ceftazidimase from klebsiella pneumoniae isolates. | | 1988 | 3049509 |
| fine structures of the capsules of klebsiella pneumoniae and escherichia coli k1. | the fine structures of the capsules of klebsiella pneumoniae and escherichia coli were determined by the rapid-freezing technique. the capsular layer was seen as a densely packed accumulation of fine fibers. the thickness of the capsule was approximately 160 nm in k. pneumoniae and less than 10 nm in e. coli k1. two layers were observed in the klebsiella capsule in which the arrangements of the fibers were different. the inner layer of the capsule was formed by a palisade of thick and dense bund ... | 1988 | 3049556 |
| bactericidal activity of human serum against strains of klebsiella from different sources. | one hundred and eighty strains of klebsiella were tested for sensitivity to pooled normal human serum, capsular type and faecal coliform reaction. strains of k. pneumoniae isolated from clinical infections were more likely to be serum resistant than those from the gut flora or environmental sources. for k. oxytoca strains, there was no significant correlation between serum sensitivity and source of isolation. of 60 strains of k. oxytoca tested, 19 (32%) were serum resistant, as were 27 (23%) of ... | 1988 | 3050105 |
| [pharmacokinetic, bacteriological and clinical studies of cefuzonam in the field of obstetrics and gynecology. study group of cefuzonam in the field of obstetrics and gynecological infections]. | a multi-center open study was conducted to investigate cefuzonam (czon, l-105) regarding to its pharmacokinetic, bacteriological and clinical aspects in the field of obstetrics and gynecology with the participation of 31 medical institutions and the related facilities. the results are summarized as follows. 1. peak mics of czon for staphylococcus aureus, coagulase (-) staphylococci, escherichia coli, klebsiella pneumoniae, bacteroides fragilis group, peptostreptococcus spp. isolated from obstetr ... | 1988 | 3050195 |
| [clinico-epidemiological study of 57 cases of klebsiella bacteremia]. | | 1988 | 3050313 |
| formation of a hexagonal lattice structure by an r-form lipopolysaccharide of klebsiella: effect of various divalent cations on the lattice formation. | the r-form lipopolysaccharide from klebsiella pneumoniae strain len-111 (o3-:k1-), from which cationic material had been removed by electrodialysis, was previously shown to form a hexagonal lattice structure with the lattice constant of 14 to 15 nm when suspended in 50 mm tris(hydroxymethyl)aminomethane buffer at ph 8.5 containing 10 mm mg2+. under this experimental condition, effects of other divalent metal cations on the hexagonal assembly of the electrodialyzed lps were compared with that of ... | 1988 | 3050376 |
| multiple subdural abscesses following colonic perforation--a rare complication of a ventriculoperitoneal shunt. | a case of colonic perforation by a ventriculoperitoneal shunt, its subsequent migration and protrusion from the anal orifice is reported. the shunt reservoir and ventricular catheter were removed percutaneously, and the disconnected peritoneal catheter was pulled out through the anus. blood culture grew klebsiella pneumoniae and streptococcus fecalis. ct scan showed multiple subdural abscesses with evidence of ventriculitis. removal of the shunt (as described), evacuation of subdural pus and sys ... | 1988 | 3050846 |
| fasciitis and abscesses complicating liposuction. | | 1988 | 3051675 |
| a bacterial factor induces changes in cysteine proteinase forms in the cellular slime mould dictyostelium discoideum. | the electrophoretic pattern of cysteine proteinases in axenically grown myxamoebae of dictyostelium discoideum can be altered by the addition of either gram-negative (klebsiella aerogenes, escherichia coli) or gram-positive (micrococcus lysodeikticus, bacillus subtilis) bacteria to the culture. no changes occurred, however, if either yeast or latex beads were used in place of bacteria. the changes involved the simultaneous loss of proteinases characteristic of the axenic cells (the a-forms) and ... | 1988 | 3052431 |
| enantioselective measurement of fungal d-arabinitol in the sera of normal adults and patients with candidiasis. | a new method was used to measure d-arabinitol enantioselectively in the sera of 27 healthy adults and four patients with candidiasis. arabinitol was measured by gas chromatography in serum that was treated with and without the klebsiella pneumoniae enzyme d-arabinitol dehydrogenase, lactic dehydrogenase, nad, and sodium pyruvate. since enzyme treatment removed 98% of 0 to 20 micrograms of d-arabinitol per ml and none of 0 to 20 micrograms of l-arabinitol per ml from spiked sera, d-arabinitol cou ... | 1988 | 3053772 |
| metastatic septic endophthalmitis in pyogenic liver abscess. | in a consecutive series of 180 patients with pyogenic liver abscess, three patients (two men and one woman, between 46 and 75 years of age) had metastatic klebsiella endophthalmitis. the incidence of metastatic endophthalmitis was 1.7% in patients with pyogenic liver abscess, 5.2% in patients with klebsiella liver abscess, and 7.8% in patients with klebsiella liver abscess having klebsiella bacteremia. despite aggressive therapeutic measures, the men permanently lost their vision and the woman e ... | 1988 | 3053874 |
| group b streptococci inhibit the chemotactic activity of the fifth component of complement. | infection with group b streptococci (gbs) is associated with a poor acute inflammatory response in which neutrophils fail to localize at the site of invasion. in the present studies, we have examined the effects of group b streptococci on c-derived chemotactic activity in human serum. fresh human serum was activated to form c5a and c5adesarg by incubation with zymosan. the activated serum was then incubated with group b organisms, centrifuged, and the supernatants tested for chemotactic activity ... | 1988 | 3053900 |
| further analysis of nitrogen fixation (nif) genes in azotobacter chroococcum: identification and expression in klebsiella pneumoniae of nifs, nifv, nifm, and nifb genes and localization of nife/n-, nifu-, nifa- and fixabc-like genes. | the results presented extend previous investigations on the genetics of nitrogen fixation in azotobacter chroococcum and indicate that nif- and fix-like dna is located in at least five different regions of the genome. region i contains functional copies of nifs,v and m, as well as nifh, d and k, all of which complemented mutants of klebsiella pneumoniae. in addition, nife- and/or nifn-like and nifu-like dna is located in this region. the organization of the nif cluster in region i closely resemb ... | 1988 | 3053983 |
| [assessment of salivary iga titer in children with recurrent respiratory infections treated with polyvalent oral vaccine. a controlled study]. | | 1988 | 3054472 |
| [comparative study of a combination of amoxicillin and clavulanic acid and a combination of ticarcillin and clavulanic acid on 249 enterobacteria]. | a comparison between the mic of amoxicillin (amx) and ticarcillin (tic) in the presence of clavulanic acid (augmentin: aug and claventin: clv) was made on 168 strains of escherichia coli resistant to amoxicillin (mic greater than 16 mg/l) and yet sensitive to ceftriaxone (mic less than or equal to 4 mg/l) and 81 strains of klebsiella pneumoniae sensitive to ceftriaxone. all those strains have been isolated between 1986-87 in bellevue hospital in saint-etienne. on 168 escherichia coli, 142 have a ... | 1988 | 3054741 |
| [in vitro antibacterial activity of apalcillin on aerobic bacteria and the regression curve]. | minimal inhibitory concentrations (mics) of apalcillin (apl) were evaluated by agar dilution on 1,201 bacterial strains isolated simultaneously in four university hospitals; agar diffusion tests (disks apl: 75 micrograms) were performed on these strains to establish relationship between mic and zone diameters. for enterobacteriaceae naturally non beta-lactamase-producing (e. coli and p. mirabilis), mode mic was 0.5 microgram/ml; some acquired penicillinase-producing strains were only inhibited b ... | 1988 | 3054743 |
| [comparison of in vitro activity of 4 oral beta-lactams on microorganisms responsible for broncho-pulmonary infections in children]. | mic 90 and mbc 90 of amoxicillin, cefaclor, cefadroxil and cefuroxime axetil have been determined by the microdilution method against 48 organisms responsible of acute respiratory tract infections in children: 17 e. coli, 15 k. pneumoniae, 16 h. influenzae. an inoculum effect and an inhibitory index in blood and bronchial secretions were determined. an inoculum effect was more important for amoxicillin and cefadroxil than for cefuroxime axetil and cefaclor. against h. influenzae, cefuroxime axet ... | 1988 | 3054759 |
| [clinical and bacteriological evaluation of cefmenoxime in the newborn infant]. | cefmenoxime (cmx) has been administered under parenteral injection to 39 neonates delivered at term, nearly always by monotherapy in an average dosage of 86.8 mg/kg/day. cmx has been used 31 times in first line therapy and 8 times after failure of association ampicillin-gentamicin. 25 strains have been identified: 16 e. coli (9 ampicillin resistant), 7 p. mirabilis (1 ampicillin resistant), 1 k. oxytoca and 1 streptococcus b. the neonates group with septicaemia (1 with arthritis) has been cured ... | 1988 | 3054762 |
| the nucleotide sequence of the nift, nify, nifx and nifw genes of k. pneumoniae. | | 1988 | 3054814 |
| klebsiella pneumoniae and other enterobacteriaceae producing novel plasmid-mediated beta-lactamases markedly active against third-generation cephalosporins: epidemiologic studies. | analysis of the enzymes produced by clinical isolates of multiresistant klebsiella pneumoniae from hospitals in france revealed two novel broad-spectrum beta-lactamases. the first, characterized by an isoelectric point of 6.3 and a high hydrolytic activity on cefotaxime, is designated ctx-1. this beta-lactamase was encoded by a 95-kilobase plasmid (incompatibility group 7m) and cotransferred with resistance to tetracyclines, sulfonamides, and aminoglycosides (aac [6']-iv). from 1984 to june 1987 ... | 1988 | 3055177 |
| plasmid-mediated resistance to third-generation cephalosporins caused by point mutations in tem-type penicillinase genes. | infections due to strains of klebsiella pneumoniae, escherichia coli, and citrobacter freundii resistant to third-generation cephalosporins have been observed recently in france and the federal republic of germany. this resistance phenotype is due to the production of new plasmid-mediated, broad-substrate-range beta-lactamases designated tem-3 to tem-7. dna-dna hybridization analysis with a probe specific for tem-1 indicated that the corresponding genes blat-3 to blat-7 were variants of the stru ... | 1988 | 3055179 |
| interactions of new plasmid-mediated beta-lactamases with third-generation cephalosporins. | the kinetic constants of three recently identified plasmid-mediated beta-lactamases--shv-2, ctx-1, and caz-1--markedly active against third-generation cephalosporins were analyzed in comparison with three better-characterized beta-lactamases--two plasmid-mediated enzymes, tem-2 and pit-2/shv-1, and r-30, a beta-lactamase from klebsiella oxytoca that has few similarities to the newer enzymes. all of these enzymes are synthesized constitutively, demonstrate efficient hydrolysis of penicillins, are ... | 1988 | 3055180 |
| some properties of serratia marcescens, salmonella paratyphi a, and enterobacter cloacae with non-enzyme-dependent multiple resistance to beta-lactam antibiotics, aminoglycosides, and quinolones. | non-enzyme-dependent multiple-drug resistance occurs preferentially in some genera of enterobacteriaceae, such as serratia, klebsiella, enterobacter, and salmonella. susceptibility to beta-lactam antibiotics, aminoglycosides, quinolones, trimethoprim, and chloramphenicol may be affected in various combinations in different mutants. proteins from the outer and inner membranes and lipopolysaccharides may be altered concomitantly. although porin alterations have been observed in all resistant mutan ... | 1988 | 3055181 |