| purification and characteristics of a virus inhibitor from dianthus caryophyllus l. ii. characterization and mode of action. | | 1962 | 13990445 |
| purification and characteristics of a virus inhibitor from dianthus caryophyllus l. i. purification and activity. | | 1962 | 13990444 |
| fluorescent antibody staining of viral antigen in dissociated plant cells. | | 1962 | 13937121 |
| the proteins of mutants of tmv: composition and structure of chemically evoked mutants of tmv rna. | | 1962 | 13994527 |
| the proteins of mutants of tmv: classification of spontaneous and chemically evoked strains. | | 1962 | 13994526 |
| enzyme resistance of complexes with ribonucleic acid with metals. | | 1962 | 13977595 |
| [theory of viruses]. | | 1951 | 14842468 |
| [artificial culture of mosaic disease microbe]. | | 2008 | 14794278 |
| [microscopic examination of plants]. | | 2008 | 14794277 |
| [attempts of virus culture in the crown gall, tumors on plant species, not yielding generalized infection]. | | 1950 | 15435027 |
| [not available]. | | 1947 | 20268263 |
| plant virus expression vectors: a powerhouse for global health. | plant-made biopharmaceuticals have long been considered a promising technology for providing inexpensive and efficacious medicines for developing countries, as well as for combating pandemic infectious diseases and for use in personalized medicine. plant virus expression vectors produce high levels of pharmaceutical proteins within a very short time period. recently, plant viruses have been employed as nanoparticles for novel forms of cancer treatment. this review provides a glimpse into the dev ... | 2017 | 28758953 |
| validation of reference genes for quantifying changes in gene expression in virus-infected tobacco. | to facilitate quantification of gene expression changes in virus-infected tobacco plants, eight housekeeping genes were evaluated for their stability of expression during infection by one of three systemically-infecting viruses (cucumber mosaic virus, potato virus x, potato virus y) or a hypersensitive-response-inducing virus (tobacco mosaic virus; tmv) limited to the inoculated leaf. five reference-gene validation programs were used to establish the order of the most stable genes for the system ... | 2017 | 28689086 |
| exogenously applied dsrna molecules deriving from the zucchini yellow mosaic virus (zymv) genome move systemically and protect cucurbits against zymv. | zucchini yellow mosaic virus (zymv) causes serious damage in a large number of cucurbits, and control measures are necessary. transgenic cucurbits expressing parts of the zymv genome have been shown to be resistant to the cognate virus. a non-transgenic approach involving the exogenous application of double-stranded rna (dsrna) has also been shown to induce resistance in tobacco against cucumber mosaic virus (cmv) and tobacco mosaic virus (tmv). in the present study, dsrna molecules derived from ... | 2017 | 28621835 |
| differential requirement of the ribosomal protein s6 and ribosomal protein s6 kinase for plant-virus accumulation and interaction of s6 kinase with potyviral vpg. | ribosomal protein s6 (rps6) is an indispensable plant protein regulated, in part, by ribosomal protein s6 kinase (s6k) which, in turn, is a key regulator of plant responses to stresses and developmental cues. increased expression of rps6 was detected in nicotiana benthamiana during infection by diverse plant viruses. silencing of the rps6 and s6k genes in n. benthamiana affected accumulation of cucumber mosaic virus, turnip mosaic virus (tumv), and potato virus a (pva) in contrast to turnip crin ... | 2017 | 28437137 |
| synthesis and antiviral activity of novel 1,4-pentadien-3-one derivatives containing a 1,3,4-thiadiazole moiety. | 1,4-pentadien-3-one derivatives derived from curcumin possess excellent inhibitory activity against plant viruses. on the basis of this finding, a series of novel 1,4-pentadien-3-one derivatives containing a 1,3,4-thiadiazole moiety were designed and synthesized, and their structures confirmed by ir, ¹h-nmr, and (13)c-nmr spectroscopy and elemental analysis. the antiviral activities of the title compounds were evaluated against tobacco mosaic virus (tmv) and cucumber mosaic virus (cmv) in vivo. ... | 2017 | 28430149 |
| adoption of the 2a ribosomal skip principle to tobacco mosaic virus for peptide display. | plant viruses are suitable as building blocks for nanomaterials and nanoparticles because they are easy to modify and can be expressed and purified using plants or heterologous expression systems. plant virus nanoparticles have been utilized for epitope presentation in vaccines, for drug delivery, as nanospheres and nanowires, and for biomedical imaging applications. fluorescent protein fusions have been instrumental for the tagging of plant virus particles. the monomeric non-oxygen-dependent fl ... | 2017 | 28702043 |
| a heterologous prime-boosting strategy with replicating vaccinia virus vectors and plant-produced hiv-1 gag/dgp41 virus-like particles. | showing modest efficacy, the rv144 hiv-1 vaccine clinical trial utilized a non-replicating canarypox viral vector and a soluble gp120 protein boost. here we built upon the rv144 strategy by developing a novel combination of a replicating, but highly-attenuated vaccinia virus vector, nyvac-kc, and plant-produced hiv-1 virus-like particles (vlps). both components contained the full-length gag and a membrane anchored truncated gp41 presenting the membrane proximal external region with its conserved ... | 2017 | 28458036 |
| mycoviruses of an endophytic fungus can replicate in plant cells: evolutionary implications. | so far there is no record of a specific virus able to infect both fungal and plant hosts in nature. however, experimental evidence shows that some plant virus rdrps are able to perform replication in trans of genomic or di rnas in the yeast saccharomyces cerevisiae. furthermore, tobacco mosaic virus was recently shown to replicate in a filamentous ascomycetous fungus. thus, at least experimentally, some plant viruses can infect some fungi. endophytic fungi have been reported from many plants and ... | 2017 | 28507331 |
| study of rubella candidate vaccine based on a structurally modified plant virus. | a novel rubella candidate vaccine based on a structurally modified plant virus - spherical particles (sps) - was developed. sps generated by the thermal remodelling of the tobacco mosaic virus are promising platforms for the development of vaccines. sps combine unique properties: biosafety, stability, high immunogenicity and the effective adsorption of antigens. we assembled in vitro and characterised complexes (candidate vaccine) based on sps and the rubella virus recombinant antigen. the candi ... | 2017 | 28511994 |
| chimeric flock house virus protein a with endoplasmic reticulum-targeting domain enhances viral replication and virus-like particle trans-encapsidation in plants. | flock house virus (fhv) rna can be trans-encapsidated, entirely in planta, by tobacco mosaic virus coat protein to form virus-like particles (vlps). vaccination with these vlps leads to strong antigen expression in mice and immune-activation. we hypothesize that creating an additional cellular site for replication and/or trans-encapsidation might significantly improve the final output of trans-encapsidated product. fhv protein a was engineered to target the endoplasmic reticulum (er) via a heter ... | 2017 | 28437636 |
| dysprosium-modified tobacco mosaic virus nanoparticles for ultra-high-field magnetic resonance and near-infrared fluorescence imaging of prostate cancer. | the increasing prevalence of ultra-high-field magnetic resonance imaging (uhfmri) in biomedical research and clinical settings will improve the resolution and diagnostic accuracy of mri scans. however, better contrast agents are needed to achieve a satisfactory signal-to-noise ratio. here we report the synthesis of a bimodal contrast agent prepared by loading the internal cavity of tobacco mosaic virus (tmv) nanoparticles with a dysprosium (dy3+) complex and the near infra-red fluorescence (nirf ... | 2017 | 28858475 |
| mussel-inspired polydopamine coating on tobacco mosaic virus: one-dimensional hybrid nanofiber for gold nanoparticles growth. | one-dimensional (1d) hybrid nanofibers with surface deposited gold nanoparticles (aunps) have been fabricated by self-assembly of rod-like tobacco mosaic virus (tmv) with mussel-inspired polymerization of dopamine and in-situ reduction of gold ion, providing a method for sensing the endocytic pathway of nanomaterial. | 2017 | 28849937 |
| synthesis and antiviral evaluation of novel 1,3,4-oxadiazole/thiadiazole-chalcone conjugates. | a series of novel 1,3,4-oxadiazole/thiadiazole-chalcone conjugates were synthesized and their in vitro and in vivo antiviral activities were evaluated via microscale thermophoresis method and half-leaf method, respectively. the in vitro results indicated that compounds 7g, 7l, 8h, and 8l displayed good antiviral activity against tmv, with the binding constant values of 5.93, 6.15, 6.02, and 5.04μm, respectively, which were comparable to that of ninnanmycin (6.78μm) and even better than that of r ... | 2017 | 28838690 |
| identification of plasmodesmal localization sequences in proteins in planta. | plasmodesmata (pd) are cell-to-cell connections that function as gateways through which small and large molecules are transported between plant cells. whereas pd transport of small molecules, such as ions and water, is presumed to occur passively, cell-to-cell transport of biological macromolecules, such proteins, most likely occurs via an active mechanism that involves specific targeting signals on the transported molecule. the scarcity of identified plasmodesmata (pd) localization signals (pls ... | 2017 | 28829412 |
| benzoylsalicylic acid derivatives as defense activators in tobacco and arabidopsis. | systemic acquired resistance (sar) is a long lasting inducible whole plant immunity often induced by either pathogens or chemical elicitors. salicylic acid (sa) is a known sar signal against a broad spectrum of pathogens in plants. in a recent study, we have reported that benzoylsalicylic acid (bzsa) is a sar inducer in tobacco and arabidopsis plants. here, we have synthesized bzsa derivatives using sa and benzoyl chlorides of various moieties as substrates. the chemical structures of bzsa deriv ... | 2017 | 28818753 |
| the use of a tobacco mosaic virus-based expression vector system in chrysanthemum. | chrysanthemums (chrysanthemum morifolium) are susceptible to tobacco mosaic virus (tmv). tmv-based expression vectors have been used in high-throughput experiments for production of foreign protein in plants and also expressing green fluorescent protein (gfp) to allow visualization of tmv movement. here, we used tmv expressing the gfp to examine the infection of chrysanthemum by a tmv-based expression vector. viral replication, movement and gfp expression by tmv-gfp were verified in upper leaves ... | 2017 | 28811760 |
| ictv virus taxonomy profile: virgaviridae. | the family virgaviridae is a family of plant viruses with rod-shaped virions, a ssrna genome with a 3'-terminal trna-like structure and a replication protein typical of alpha-like viruses. differences in the number of genome components, genome organization and the mode of transmission provide the basis for genus demarcation. tobacco mosaic virus (genus tobamovirus) was the first virus to be discovered (in 1886); it is present in high concentrations in infected plants, is extremely stable and has ... | 2017 | 28786782 |
| programming self-assembly of tobacco mosaic virus coat proteins at pickering emulsion interfaces for nanorod-constructed capsules. | pickering emulsion constructions on nanorods with high aspect ratio are a great challenge because of the geometry restrictions. on the basis of the theory that the stability of pickering emulsion is highly dependent on the size and amphiphilicity of the nanoparticle at fluid interfaces, we report a novel strategy to fabricate long-time stable pickering emulsion consisting of tobacco mosaic virus (tmv)-like nanorods through the programming self-assembly of tmv coat protein (tmvcp). the first step ... | 2017 | 28783309 |
| biogenic and synthetic peptides with oppositely charged amino acids as binding sites for mineralization. | proteins regulate diverse biological processes by the specific interaction with, e.g., nucleic acids, proteins and inorganic molecules. the generation of inorganic hybrid materials, such as shell formation in mollusks, is a protein-controlled mineralization process. moreover, inorganic-binding peptides are attractive for the bioinspired mineralization of non-natural inorganic functional materials for technical applications. however, it is still challenging to identify mineral-binding peptide mot ... | 2017 | 28772478 |
| anti-tmv activity and functional mechanisms of two sesquiterpenoids isolated from tithonia diversifolia. | unlike chemical pesticides, antiviral plants are biodegradable, replenishable and safe. in this study, 14 sesquiterpene compounds from tithonia diversifolia were tested for their activities against tobacco mosaic virus (tmv) using the half-leaf method. tagitinin c (ses-2) and 1β-methoxydiversifolin-3-0-methyl ether (ses-5) were found to have in vivo curative activities of 62.86% and 60.27% respectively, at concentrations of 100μg/ml, respectively. in contrast, the in vivo curative inhibition rat ... | 2017 | 28755690 |
| shear flow induced long-range ordering of rod-like viral nanoparticles within hydrogel. | controlling the alignment of anisotropic nanoparticles within a three-dimensional (3d) environment over large-scale is still a challenge. in this paper, a facile method to align rod-like nanoparticles in hydrogel via shear flow to afford a long-range order is reported. the rod-like tobacco mosaic virus (tmv) was employed as a prototypical anisotropic particles in the study, and the shear force provided by the unidirectional flow of the precursor solution direct the alignment of tmvs, which could ... | 2017 | 28755559 |
| synthesis, antiviral activity, and molecular docking study of trans-ferulic acid derivatives containing acylhydrazone moiety. | in this study, we report the synthesis and antiviral activity of trans-ferulic acid derivatives containing acylhydrazone moiety. biological tests demonstrated that most target compounds showed potent antiviral activity against tobacco mosaic virus (tmv). compound d4 showed remarkable inactivating activity with ec50 value of 36.59μg/ml, which was obviously superior to ribavirin (126.05μg/ml). molecular docking results revealed that compound d4 exhibited the optimal combining capacity with five hy ... | 2017 | 28751145 |
| poxylation as an alternative stealth coating for biomedical applications. | polyethylene glycol (peg) polymers are currently used in a variety of medical formulations to reduce toxicity, minimize immune interactions and improve pharmacokinetics. despite its widespread use however, the presence of anti-peg antibodies indicates that this polymer has the potential to be immunogenic and antigenic. here we present an alternative polymer, poly(2-oxazoline) (pox) for stealth applications, specifically shielding of a proteinaceous nanoparticle from recognition by the immune sys ... | 2017 | 28713172 |
| tobacco mosaic virus infection disproportionately impacts phloem associated translatomes in arabidopsis thaliana and nicotiana benthamiana. | in this study we use vascular specific promoters and a translating ribosome affinity purification strategy to identify phloem associated translatome responses to infection by tobacco mosaic virus (tmv) in systemic hosts arabidopsis thaliana ecotype shahdara and nicotiana benthamiana. results demonstrate that in both hosts the number of translatome gene alterations that occurred in response to infection is at least four fold higher in phloem specific translatomes than in non-phloem translatomes. ... | 2017 | 28710959 |
| cardr1, an rna-dependent rna polymerase plays a positive role in pepper resistance against tmv. | rna silencing functions as a major natural antiviral defense mechanism in plants. rna-dependent rna polymerases (rdrs) that catalyze the synthesis of double-stranded rnas, are considered as a fundamental element in rna silencing pathways. in arabidopsis thaliana, rdr1, 2 and 6 play important roles in anti-viral rna silencing. expression of rdr1 can be elevated following plant treatment with defense hormones and virus infection. rdr1 has been studied in several crop species, but not in pepper (ca ... | 2017 | 28702034 |
| featured article: delivery of chemotherapeutic vcmmae using tobacco mosaic virus nanoparticles. | the first-line treatment for non-hodgkin's lymphoma is chemotherapy. while generally well tolerated, off-target effects and chemotherapy-associated complications are still of concern. to overcome the challenges associated with systemic chemotherapy, we developed a biology-inspired, nanoparticle drug delivery system (nanodds) making use of the nucleoprotein components of the tobacco mosaic virus (tmv). virus-based nanoparticles, including the high-aspect ratio soft nanorods formed by tmv, are gro ... | 2017 | 28675044 |
| differential expression of novel micrornas in response to the infection of a tmv mutant with an internal poly(a) tract in n. benthamiana. | we first constructed small rna libraries of tmv- and tmv-43a-infected n. benthamiana for high throughput sequencing. a total number of 181 novel micrornas (mirnas) were identified through an improved mirnas analysis pipeline. we were able to identify consistent mirna expression changes induced in tmv and tmv-43a-infected plants, as well as differences associated with the upd substitution in the tmv-43a viral genome. virally induced mirnas are associated with distinct processes and functions of p ... | 2017 | 28668702 |
| multiplexed gene editing and protein overexpression using a tobacco mosaic virus viral vector. | development of crispr/cas9 transient gene editing screening tools in plant biology has been hindered by difficulty of delivering high quantities of biologically active single guide rnas (sgrnas). furthermore, it has been largely accepted that in vivo generated sgrnas need to be devoid of extraneous nucleotides, which has limited sgrna expression by delivery vectors. here, we increased cellular concentrations of sgrna by transiently delivering sgrnas using a tobacco mosaic virus-derived vector (t ... | 2017 | 28663331 |
| application of fatty acids as antiviral agents against tobacco mosaic virus. | numerous studies reported fatty acids (fas) affecting basal resistance to bacterial and fungal pathogens in plants, but limited reports focused on antiviral agents. in this study, oleic acid was separated from cottonseed oil sludge by antiviral bioassay-guided methods. antiviral activity of fas was compared with that of ningnanmycin. subsequently, effects of fas on defense-related enzymes (pal, pod) and defense-related genes (pr-1a, pr-5) were studied. fas presented moderate antiviral activity, ... | 2017 | 28595927 |
| the plant cellular systems for plant virus movement. | plasmodesmata (pds) are specialized intercellular channels that facilitate the exchange of various molecules, including sugars, ribonucleoprotein complexes, transcription factors, and mrna. their diameters, estimated to be 2.5 nm in the neck region, are too small to transfer viruses or viral genomes. tobacco mosaic virus and potexviruses are the most extensively studied viruses. in viruses, the movement protein (mp) is responsible for the pd gating that allows the intercellular movement of viral ... | 2017 | 28592941 |
| tobamovirus 3'-terminal gene overlap may be a mechanism for within-host fitness improvement. | overlapping genes (ogs) are a universal phenomenon in all kingdoms, and viruses display a high content of ogs combined with a high rate of evolution. it is believed that the mechanism of gene overlap is based on overprinting of an existing gene. ogs help virus genes compress a maximum amount of information into short sequences, conferring viral proteins with novel features and thereby increasing their within-host fitness. analysis of tobamovirus 3'-terminal genes reveals at least two modes of og ... | 2017 | 28553276 |
| plant bax inhibitor-1 interacts with atg6 to regulate autophagy and programmed cell death. | autophagy is an evolutionarily conserved catabolic process and is involved in the regulation of programmed cell death during the plant immune response. however, mechanisms regulating autophagy and cell death are incompletely understood. here, we demonstrate that plant bax inhibitor-1 (bi-1), a highly conserved cell death regulator, interacts with atg6, a core autophagy-related protein. silencing of bi-1 reduced the autophagic activity induced by both n gene-mediated resistance to tobacco mosaic ... | 2017 | 28537463 |
| biofabrication of tobacco mosaic virus-nanoscaffolded supercapacitors via temporal capillary microfluidics. | this paper reports the implementation of temporal capillary microfluidic patterns and biological nanoscaffolds in autonomous microfabrication of nanostructured symmetric electrochemical supercapacitors. a photoresist layer was first patterned on the substrate, forming a capillary microfluidics layer with two separated interdigitated microchannels. tobacco mosaic virus (tmv) macromolecules suspended in solution are autonomously delivered into the microfluidics, and form a dense bio-nanoscaffolds ... | 2017 | 28524067 |
| inactivation of tobacco mosaic virus using gamma irradiation and its potential modes of action. | gamma irradiation is a non-thermal processing technique used to disinfect harmful microorganisms in agriculture. this technology has been shown to be an effective method to control bacterial and fungal plant pathogens. however, its effect on viral plant pathogen is less understood. gamma irradiation was evaluated for the inactivation of tobacco mosaic virus (tmv). tmv infectivity has gradually decreased following irradiation in a dose-dependent manner and virus was completely inactivated at a do ... | 2017 | 28523930 |
| calculation of the cross-sectional shape of a fibril from equatorial scattering. | an alternate formulation of helical diffraction theory is used to generate cross-sectional shapes of fibrous structures from equatorial scattering. we demonstrate this approach with computationally generated scattering intensities and then apply it to scattering data from tobacco mosaic virus (tmv) and in vitro assembled fibrils of aβ40 peptides. refining the cross-sectional shape of tmv from saxs data collected on a 26mg/ml solution resulted in a circular shape with outer diameter of ∼180å and ... | 2017 | 28511991 |
| a novel hairpin library-based approach to identify nbs-lrr genes required for effector-triggered hypersensitive response in nicotiana benthamiana. | pti and eti are the two major defence mechanisms in plants. eti is triggered by the detection of pathogen effectors, or their activity, in the plant cell and most of the time involves internal receptors known as resistance (r) genes. an increasing number of r genes responsible for recognition of specific effectors have been characterised over the years; however, methods to identify r genes are often challenging and cannot always be translated to crop plants. | 2017 | 28465712 |
| high aspect ratio nanotubes formed by tobacco mosaic virus for delivery of photodynamic agents targeting melanoma. | melanoma is a highly aggressive cancer that is unresponsive to many traditional therapies. recently, photodynamic therapy has shown promise in its treatment as an adjuvant therapy. however, conventional photosensitizers are limited by poor solubility and limited accumulation within target tissue. here, we report the delivery of a porphyrin-based photosensitizer encapsulated within a plant viral nanoparticle. specifically, we make use of the hollow, high aspect ratio nanotubes formed by the nucle ... | 2016 | 28713855 |
| a new transient expression system for large-scale production of recombinant proteins in plants based on air-brushing an agrobacterium suspension. | plant transient expression using virus-based vectors is advantageous when high level of gene expression is desired within a short time. in this study, a new system, named "air-brush," has been developed to facilitate a scale-up production of recombinant proteins in plants. gfp was expressed successfully in nicotiana benthamiana (nb) plants by air-brushing an agrobacterium suspension that contained the tmv-based vector p35s-30b-gfp. key factors influencing the gene expression were optimized, incl ... | 2015 | 28626695 |