| molecular identification of wheat endoxylanase inhibitor taxi-ii and the determinants of its inhibition specificity. | wheat grains contain triticum aestivum xylanase inhibitor (taxi) proteins which inhibit microbial xylanases, some of which are used in cereal based food industries. these inhibitors may play a role in plant defence. among the taxi isoforms described so far, taxi-ii displays a deviating inhibition specificity pattern. here, we report on the molecular identity of taxi-ii and the basis of its inhibition specificity. three candidate taxi-ii encoding sequences were isolated and recombinantly expresse ... | 2005 | 16084833 |
| study of spanish grape mycobiota and ochratoxin a production by isolates of aspergillus tubingensis and other members of aspergillus section nigri. | the native mycobiota of five grape varieties grown in spain has been studied. four (bobal, tempranillo, garnacha, and monastrell) were red varieties and one (moscatel) was white. the main fungal genera isolated were alternaria, cladosporium, and aspergillus. the isolation frequency of aspergillus spp. section nigri in contaminated samples was 82%. ochratoxin a (ota) production was assessed using yeast extract-sucrose broth supplemented with 5% bee pollen. cultures of 205 isolates from this secti ... | 2005 | 16085865 |
| transformation of ginsenosides rb1 and re from panax ginseng by food microorganisms. | ginsenosides rb1 and re, respectively belonging to the major protopanaxadiol and protopanaxatriol ginsenosides, were transformed using cell-free extracts from food microorganisms. rb1 was transformed into compound k via rd and f2 by bifidobacterium sp. int57, bif. sp. sj32, aspergillus niger and a. usamii. lactobacillus delbrueckii, and leuconostoc paramesenteroides transformed rb1 into rh2 via rd and f2. bifidobacterium sp. sh5 transformed rb1 into f2 via rd. re was transformed into rh1 via rg2 ... | 2005 | 16086257 |
| biological control of collar rot disease with broad-spectrum antifungal bacteria associated with groundnut. | bacteria associated with 6 habitats of groundnut were evaluated for their broad-spectrum antifungal activity and suppression of collar rot (aspergillus niger) of groundnut. three hundred and ninety-three strains were tested against 8 fungal pathogens of groundnut including 5 necrotrophic fungi, aspergillus flavus, a. niger, rhizoctonia bataticola, rhizoctonia solani, and sclerotium rolfsii, and 3 biotrophic fungi, cercospora arachidicola, phaeoisariopsis personata, and puccinia arachidis. pseudo ... | 2005 | 16091770 |
| overproduction and characterization of xylanase b from aspergillus niger. | the xynb gene, which encodes endo-beta-1,4-xylanase xynb, in aspergillus niger brfm281 was amplified by rt-pcr using mrna isolated from a culture containing sugar beet pulp as an inducer. the cdna was cloned into an expression cassette under the control of the strong and constitutive glyceraldhehyde-3-phosphate dehydrogenase gene promoter. the expression system was designed to produce the recombinant enzyme xynb with a six-histidine peptide fused to the carboxy end of the protein. homologous ove ... | 2005 | 16091777 |
| stable encapsulation of active enzyme by application of multilayer nanofilm coatings to alginate microspheres. | in an effort to improve the stability for long-term biosensor use, layer-by-layer self-assembly was explored as a potential technique to provide a diffusion barrier to encapsulated glucose oxidase inside alginate microspheres (<5 microm), fabricated using an emulsification technique. the total loss of encapsulated enzyme was reduced to less than 25 and 15% with the application of single pah/pss and crosslinked pah/paa coatings, respectively, in comparison to at least a 45% loss observed with unc ... | 2005 | 16096991 |
| use of adapted aspergillus niger in the bioleaching of spent refinery processing catalyst. | spent refinery processing catalyst is listed as a hazardous waste; the toxicity characteristic leaching procedure (tclp) extracts of the catalyst are found to contain heavy metals at concentrations exceeding the regulated levels. in the present investigation, aspergillus niger was adapted to single metal ions ni, mo or al (at 100-2,000 mg/l in steps of 100mg/l) and then to a mixture of ni, mo and al (at a mass ratio of 1:2:6, as approximately present in the spent catalyst). adaptation experiment ... | 2006 | 16105700 |
| [analysis of the mechanism of anti-mycotic activities by azoles potentiated by new erythromycin derivatives]. | | 2004 | 16108609 |
| incidence of invasive aspergillosis following hematopoietic stem cell and solid organ transplantation: interim results of a prospective multicenter surveillance program. | the incidence of invasive aspergillosis was estimated among 4621 hematopoietic stem cell transplants (hsct) and 4110 solid organ transplants (sot) at 19 sites dispersed throughout the united states, during a 22 month period from 1 march 2001 through 31 december 2002. cases were identified using the consensus definitions for proven and probable infection developed by the invasive fungal infections cooperative group of the european organization for research and treatment of cancer and the mycoses ... | 2005 | 16110792 |
| [construction and functional analysis of the pepb gene disruptant in aspergillus niger]. | an integration plasmid pmw1-pepb for the pepb gene disruption in aspergillus was constructed. the plasmid contained the pepb gene upstream (p) 1.4kb and downstream (t) 1.3kb homologous fragments with insertion of the expression unit of the hygromycin resistance gene (hph) between them. p and t dna fragments were synthesized by pcr from aspergillus niger chromosomal dna. the integration plasmid was digested with the hpa i restriction enzyme, the resultant 4.2kb linear fragment was introduced into ... | 2004 | 16110957 |
| enzymatic detergent formulation containing amylase from aspergillus niger: a comparative study with commercial detergent formulations. | there is a wide range of biotechnological applications for amylases, including the textile, pharmaceutical, food and laundry industries. hydrolytic enzymes are 100% biodegradable and enzymatic detergents can achieve effective cleaning with lukewarm water. microorganisms and culture media were tested for amylase production and the best producer was aspergillus niger l119 (3.9 u ml(-1) +/- 0.2) in submerged culture and its amylase demonstrated excellent activity at 50-55 degrees c and ph 4.0, rema ... | 2006 | 16112858 |
| antimicrobial activities of barringtonia acutangula. | crude extracts and vlc fractions from the stem bark of barringtonia acutangula (l.) gaertn (fam. lecythidaceae) were screened for their antimicrobial activities against two gram-positive bacteria, two gram-negative bacteria and two fungi using a microdilution titre assay. among the crude extracts, petroleum ether extract showed good activity against all test organisms. the vlc fraction pe 16 was found to be very effective against bacillus subtilis (mic=25 microg/ml) and aspergillus niger (mic=12 ... | 2005 | 16114086 |
| biodegradation of crude petroleum and petroleum products by fungi isolated from two oil seeds (melon and soybean). | crude petroleum oil degrading fungi were isolated from two oil seeds, cucumeropsis mannii (melon) and glycine max (soybean) seeds in the presence and absence of petroleum fumes. an assessment of the relative ability of each fungus to degrade crude petroleum, diesel and kerosene on minimum salt solution was done using change in optical density read on spectrophotometer. twenty-one fungal species (14 genera) were isolated altogether during this experiment. these include eight species of aspergillu ... | 2005 | 16114459 |
| synthesis and antifungal activity of naphthalene-1,4-diones modified at positions 2, 3, and 5. | a series of 2-arylamino-5-hydroxy-naphthalene-1,4-diones, 3-arylamino-5-methoxy-naphthalene-1,4-diones, and 2-arylamino-3chloro-5-hydroxy-naphthalene-1,4-diones were synthesized and tested for in vitro antifungal activity against the species candida and aspergillus niger. among those tested, 3-arylamino-5-methoxy-naphthalene-1,4-diones exhibited potent antifungal activity. in general, the 3-arylamino-5-methoxy-naphthalene-1,4-diones showed more potent antifungal activity than the 2-arylamino-5-h ... | 2005 | 16114486 |
| [invasive pulmonary aspergillesis]. | to evaluate the results of therapy of invasive pulmonary aspergillesis (ipa) in one medical center from 2000 to 2005. | 2005 | 16116913 |
| [possible sources of aspergilla infection in a hematological hospital]. | to investigate spread, species spectrum, quantity of aspergilla spores in the air of a hematological hospital. | 2005 | 16116914 |
| the contribution of ionic interactions to the conformational stability and function of polygalacturonase from a. niger. | aspergillus niger produces multiple forms of polygalacturonases with molecular masses ranging from 30 to 60 kda. the high molecular weight polygalacturonase (61+/-2 kda) from a. niger possesses a ph optimum of 4.3 and a pi of 3.9. the enzyme exhibited high sensitivity, both in terms of activity and structure, in the ph range of 4.3-7.0. the enzyme was irreversibly inactivated at ph 7.0. the enzyme is predominantly rich in parallel beta structure. there is unfolding of the enzyme molecule between ... | 2005 | 16122785 |
| probing the determinants of substrate specificity of a feruloyl esterase, anfaea, from aspergillus niger. | feruloyl esterases hydrolyse phenolic groups involved in the cross-linking of arabinoxylan to other polymeric structures. this is important for opening the cell wall structure making material more accessible to glycoside hydrolases. here we describe the crystal structure of inactive s133a mutant of type-a feruloyl esterase from aspergillus niger (anfaea) in complex with a feruloylated trisaccharide substrate. only the ferulic acid moiety of the substrate is visible in the electron density map, s ... | 2005 | 16128806 |
| biosorption of cadmium by fungal biomass of aspergillus niger. | to investigate the removal of cadmium from aqueous solution by waste fungal biomass of aspergillus niger, originated from citric acid fermentation industry. | 2005 | 16131014 |
| location of glucose oxidase during production by aspergillus niger. | the production of the enzyme glucose oxidase by aspergillus niger is well documented. however, its distribution within the fungal culture is less well defined. since the enzyme location impacts significantly on enzyme recovery, this study quantifies the enzyme distribution between the extracellular fluid, cell wall, cytoplasm and slime mucilage fractions in an a. niger nrrl-3. the culture was separated into the individual fractions and the glucose oxidase activity was determined in each. the ext ... | 2006 | 16133329 |
| oxygen profiles and biomass distribution in biopellets of aspergillus niger. | morphology of fungal pellets has a significant influence on mass transfer and turnover processes in submerged cultures. there are many reports in literature that biomass is not distributed homogeneously over the pellet radius, yet quantitative data is rare. this study presents a method for the quantification of fungal pellet structure (aspergillus niger). confocal laser scanning microscopy (clsm) is used in combination with image analysis freeware (image j). hyphal distribution is resolved spati ... | 2005 | 16136592 |
| post-lung transplantation aspergillus niger infection. | | 2005 | 16143277 |
| total synthesis and further scrutiny of the in vitro antifungal activity of 6-nonadecynoic acid. | the antifungal, naturally occurring acetylenic fatty acid 6-nonadecynoic acid was synthesized in three steps (18 % overall yield), for the first time starting with commercially available 1-tetradecyne. the synthesis developed herein will facilitate the further study of the antifungal properties of this naturally occurring acetylenic fatty acid. the 6-nonadecynoic acid exhibited the best antifungal activity (< 4.3 microm) against cryptococcus neoformans atcc 66031 in sabouraud dextrose broth (sdb ... | 2005 | 16143955 |
| microencapsulated mycelium-bound tannase from aspergillus niger: an efficient catalyst for esterification of propyl gallate in organic solvents. | microencapsulated aspergillus niger with mycelium-bound tannase activity was employed to investigate the esterification of propyl gallate from gallic acid and propanol in organic solvents. the effects of various organic solvents (log p: -1.0 to 6.6) on the enzymatic reactions showed that benzene (log p: 2.0) was the suitable solvent, for which the conversion reached 26.8%. the optimum catalyst concentration and water concentration was found at 25 capsules in 10 ml of benzene and 0.04 g of water/ ... | 2005 | 16148363 |
| cloning and characterization of a tyrosinase gene from the white-rot fungus pycnoporus sanguineus, and overproduction of the recombinant protein in aspergillus niger. | a new tyrosinase-encoding gene (2,204 bp) and the corresponding cdna (1,857 nucleotides) from the white-rot fungus pycnoporus sanguineus brfm49 were cloned. this gene consisted of seven exons and six introns and encoded a predicted protein of 68 kda, exceeding the mature tyrosinase by 23 kda. p. sanguineus tyrosinase cdna was over-expressed in aspergillus niger, a particularly suitable fungus for heterologous expression of proteins of biotechnological interest, under the control of the glycerald ... | 2006 | 16151802 |
| preparation of a very stable immobilized biocatalyst of glucose oxidase from aspergillus niger. | glucose oxidase (gox) has been immobilized on different activated supports, including glyoxyl agarose, epoxy sepabeads and glutaraldehyde-activated supports. immobilization onto supports pre-activated with glutaraldehyde rendered the most thermo-stable preparation of gox. therefore, as the glutaraldehyde chemistry gave a high stabilization of the enzyme, we proposed another technique for improving the multipoint attachment through glutaraldehyde: the enzyme was ionically adsorbed on cationic sup ... | 2006 | 16153734 |
| biodegradation of high concentrations of hexadecane by aspergillus niger in a solid-state system: kinetic analysis. | solid-state microcosms were used to assess the influence of constant and variable c/n ratios on the biodegradation efficiency by aspergillus niger at high hexadecane (hxd) concentrations (180-717 mg g-1). with a constant c/n ratio, 100% biodegradation (33-44% mineralization) was achieved after 15 days, at rates increasing as the hxd concentration increased. biomass yields (yx/s) remained almost independent (approximately 0.77) of the carbon-source amount, while the specific growth rates (mu) dec ... | 2006 | 16153825 |
| characterisation of cwpa, a putative glycosylphosphatidylinositol-anchored cell wall mannoprotein in the filamentous fungus aspergillus niger. | glycosylphosphatidylinositol (gpi)-anchored proteins in fungi are found at the cell surface, either as plasma membrane proteins (gpi-pmps) or attached by a remnant of the gpi-anchor to the cell wall (gpi-cwps). gpi-cwps can be extracted from the cell wall by treatment with hydrofluoric acid (hf), which cleaves the phosphodiester bond that is present in the remnant of the gpi-anchor. the filamentous fungus aspergillus niger contains at least seven hf-extractable cell wall mannoproteins. one gene ... | 2005 | 16154783 |
| purification of different lipases from aspergillus niger by using a highly selective adsorption on hydrophobic supports. | in this manuscript, we have purified three different lipases from crude preparations from aspergillus niger in a simple fashion, secluding the esterases and other enzymes presented in the preparation. firstly, the crude was offered at low ionic strength to octyl agarose. the support specifically adsorbed two lipases, with molecular weights of 43 and 65 kda. desorption with a gradient of triton x-100 permitted to fully purify both lipases. the addition of octadecyl-sepabeads support to the non-ad ... | 2005 | 16155948 |
| purification and characterization of a novel pyrethroid hydrolase from aspergillus niger zd11. | the pyrethroid pesticides residues on foods and environmental contamination are a public safety concern. pretreatment with pyrethroid hydrolase has the potential to alleviate the conditions. for this purpose, a fungus capable of using pyrethroid pesticides as a sole carbon source was isolated from the soil and characterized as aspergillus niger zd11. a novel pyrethroid hydrolase from cell extract was purified 41.5-fold to apparent homogeneity with 12.6% overall recovery. it is a monomeric struct ... | 2005 | 16159167 |
| upr-independent dithiothreitol stress-induced genes in aspergillus niger. | a subtraction library was prepared from cultures of aspergillus niger that had or had not been exposed to dithiothreitol (dtt), in order to identify genes involved in the unfolded protein response (upr) or in the response to reductive stress. a large fraction of the clones in the library (40%) encoded two putative methyltransferases (mts) whose function has yet to be determined. other stress-responsive genes included a homologue of the mn2+-containing superoxide dismutase gene (sodb) and a numbe ... | 2005 | 16160852 |
| investigations on anti-aspergillus properties of bacterial products. | to investigate the anti-aspergillus properties of bacterial products. | 2005 | 16162136 |
| role of antioxidant enzymes in survival of conidiospores of aspergillus niger 26 under conditions of temperature stress. | a better understanding of the role of antioxidant enzymes, superoxide dismutase (sod) and catalase (cat) in the protection of aspergillus niger spores against thermal stress. | 2005 | 16162242 |
| fever and progressive respiratory failure in three elderly family members. | | 2005 | 16162796 |
| the aspergillus niger mads-box transcription factor rlma is required for cell wall reinforcement in response to cell wall stress. | in aspergillus niger, the genes coding for glutamine:fructose-6-phosphate amidotransferase (gfaa) and alpha-1,3-glucan synthase (agsa) are induced in response to cell wall stress. in silico analysis of the promoter region of the two genes revealed the presence of putative dna binding sites for transcription factors involved in stress responses, including sites identical to the saccharomyces cerevisiae rlm1p and msn2p/msn4p transcription factors. promoter analysis indicated that the induction of ... | 2005 | 16164567 |
| screening and identification of the levoglucosan kinase gene (lgk) from aspergillus niger by lc-esi-ms/ms and rt-pcr. | a protein of 75,000 daltons with levoglucosan kinase activity was purified from aspergillus niger. after in-gel digestion by trypsin, a 14-mer peptide was sequenced and analyzed by lc-esi-ms/ms. using a primer derived from the 14-mer peptide in combination with oligo-(dt)18, a cdna fragment was obtained by rt-pcr. a search of the genbank database indicated that the protein had not been identified before. a similar protein named hypothetical protein fg07802.1 (eaa77996.1) was found to exist in gi ... | 2005 | 16165323 |
| evaluation of a flexible new liquid polymer wound dressing. | occlusive dressings are the foundation of wound care and have been shown to speed epithelialization and healing of surgical sites. many different dressings have been introduced over the years including antibacterial ointments with gauze and adhesive, hydrocolloid bandages, and liquid adhesives. all of these have their limitations and advantages. we introduce our experience with a new organic polymer in solvent which when applied to a wound forms a flexible occlusive bandage. the material has bee ... | 2005 | 16167416 |
| production, acceptability and microbiological evaluation of weaning food formulations. | weaning food formulations were produced from pearl millet cultivar (sosat c-88), cowpea and groundnut in the ratio of 70:20:10 and 5 per cent malt from six pearl millet cultivars (sosat c-88, zango, ex-borno, icmv is 94206, gwagwa, gb 8735) and one sorghum cultivar (icsv iii) were added to produce seven formulations plus the one without malt. microbiological quality of eight weaning food formulations was evaluated. twenty weaning mothers were used to determine the acceptability of gruels from th ... | 2006 | 16169859 |
| nitric oxide mediates the fungal elicitor-induced hypericin production of hypericum perforatum cell suspension cultures through a jasmonic-acid-dependent signal pathway. | fungal elicitor prepared from the cell walls of aspergillum niger induces multiple responses of hypericum perforatum cells, including nitric oxide (no) generation, jasmonic acid (ja) biosynthesis, and hypericin production. to determine the role of no and ja in elicitor-induced hypericin production, we study the effects of no scavenger 2- to 4-carboxyphenyl-4,4, 5,5-tetramethylimidazoline-1-oxyl-3-oxide (cpito), nitric oxide synthase inhibitor s,s'-1,3-phenylene-bis(1,2-ethanediyl)-bis-isothioure ... | 2005 | 16169960 |
| non-dermatophytic molds as agents of onychomycosis in izmir, turkey - a prospective study. | the purpose of this study was to determine the prevalence of causative non-dermatophytic filamentous fungi in onychomycosis. totally 1,222 (1,222 x 3 = 3,666) samples of nail scrapings from 1,146 patients (from 76 patients two specimens: both from finger- and toe-nails) with prediagnosis of onychomycosis sent to the mycology laboratory from the clinic of dermatology, ege university hospital, izmir, turkey, july 2001-december 2003, were prospectively studied with conventional mycological procedur ... | 2005 | 16170607 |
| monoterpene lactones from the seeds of nephelium lappaceum. | dichloromethane extracts of the seeds of nephelium lappaceum afforded two new diastereomeric monoterpene lactones, 1 and 2, and the known butenolide siphonodin (3), as well as kaempferol 3-o-beta-d-glucopyranoside-7-o-alpha-l-rhamnopyranoside. compounds 1 and 2 represent a new monoterpene skeleton, and their structures were elucidated by extensive 1d and 2d nmr spectral data interpretation. antimicrobial testing was carried out on 1 and 2 against a panel of bacteria and fungi. | 2005 | 16180821 |
| quantitative study of electrostatic and steric effects on physicochemical property and biological activity. | the physicochemical properties and biological activities are highly dependent on the electrostatic and steric effects of compounds. the characterization of these structural features will help to elucidate the quantitative structure-property/activity relationship (qspr/qsar). in this paper, two novel structural descriptors, lone-pair electrons index (lei) and molecular volume index (mvi) were developed to quantify the molecular electrostatic and steric effects, respectively. one of the most attra ... | 2006 | 16182578 |
| effective prevention of chill-haze in beer using an acid proline-specific endoprotease from aspergillus niger. | chill-haze formation during beer production is known to involve polyphenols that interact with proline-rich proteins. we hypothesized that incubating beer wort with a proline-specific protease would extensively hydrolyze these proline-rich proteins, yielding a peptide fraction that is unable to form a haze. predigestion of the proline-rich wheat gliadin with different proteases pointed toward a strong haze-suppressing effect by a proline-specific enzyme. this finding was confirmed in small-scale ... | 2005 | 16190654 |
| extracellular prolyl endoprotease from aspergillus niger and its use in the debittering of protein hydrolysates. | the observation that the bitterest peptides from casein hydrolysates contain several proline residues led us to hypothesize that a proline-specific protease would be instrumental in debittering such peptides. to identify the desired proline-specific activity, a microbiological screening was carried out in which the chromogenic peptide benzyloxycarbonyl-glycine-proline-p-nitroanilide (z-gly-pro-pna) was used as the substrate. an aspergillus niger (a. niger) strain was identified that produces an ... | 2005 | 16190655 |
| surveillance of toxigenic fungi and ochratoxin a in feedstuffs from córdoba province, argentina. | the aim of this work was to evaluate the incidence of potential ochratoxigenic mycoflora and ochratoxin a (oa) in poultry, pig and rabbit feeds. eighty poultry, pig and rabbit feed samples were taken at random from factories located from córdoba province, argentina, over a period of 8 months. isolation and quantitative enumeration of fungal propagules were done on drbc and dg18 media. the predominant species were a. candidus, a. flavus, a. terreus, a. parasiticus, p. implicatum, p. minioluteum, ... | 2005 | 16195938 |
| cross-reactivity of aspergillus, penicillium, and stachybotrys antigens using affinity-purified antibodies and immunoassay. | in this study, the author examined the cross-reactivities of stachybotrys chartarum, aspergillus niger/fumigatus, and penicillium notatum with affinity-purified rabbit sera. the molds were grown for expression of maximum numbers of antigens, after which they were extracted and mixed with commercially available extracts. the mixture was used for antibody preparation in rabbits, measurement of antibody levels, and for the demonstration of the degree of cross-reactivity. control rabbits were inject ... | 2004 | 16201672 |
| characteristics of transxylosylation by beta-xylosidase from aspergillus awamori k4. | the aspergillus awamori k4 beta-xylosidase gene (xaw1) sequence was deduced by sequencing rt-pcr and pcr products. the orf was 2,412 bp and the predicted peptide was 804 amino acids long, corresponding to a molecular weight of 87,156 da. the mature protein was 778 amino acids long with a molecular weight of 84,632 da. a homology search of the amino acid sequence revealed that it was very similar to the aspergillus niger beta-xylosidase gene with only five amino acid differences. k4 beta-xylosida ... | 2005 | 16202538 |
| specific ion effects on the growth rates of staphylococcus aureus and pseudomonas aeruginosa. | motivated by recent advances in the physical and chemical basis of the hofmeister effect, we measured the rate cell growth of s. aureus--a halophilic pathogenic bacterium--and of p. aeruginosa, an opportunistic pathogen, in the presence of different aqueous salt solutions at different concentrations (0.2, 0.6 and 0.9 m). microorganism growth rates depend strongly on the kind of anion in the growth medium. in the case of s. aureus, chloride provides a favorable growth medium, while both kosmotrop ... | 2005 | 16204851 |
| effect of fermentation system on the production and properties of tannase of aspergillus niger van tieghem mtcc 2425. | the tannase-producing efficiency of liquid-surface fermentation (lsf) and solid-state fermentation (ssf) vis-à-vis submerged fermentation (smf) was investigated in a strain of aspergillus niger, besides finding out if there was a change in the activity pattern of tannase in these fermentation processes. the studies on the physicochemical properties were confined to intracellular tannase as only this form of enzyme was produced by a. niger in all three fermentation processes. in lsf and smf, the ... | 2005 | 16205027 |
| development of a dna microarray for detection and identification of fungal pathogens involved in invasive mycoses. | invasive fungal infections have emerged as a major cause of morbidity and mortality in immunocompromised patients. conventional identification of pathogenic fungi in clinical microbiology laboratories is time-consuming and, therefore, often imperfect for the early initiation of an adequate antifungal therapy. we developed a diagnostic microarray for the rapid and simultaneous identification of the 12 most common pathogenic candida and aspergillus species. oligonucleotide probes were designed by ... | 2005 | 16207946 |
| a potent chitinolytic activity of alternaria alternata isolated from egyptian black sand. | eight fungal species characterized by chitinolytic activity were isolated from egyptian black sand collected from rosetta coast. genus aspergillus and alternaria alternata exhibited the highest density (> 40% of the total count, each) on the isolation plates containing different treatments of native shrimp shell chitin. genus aspergillus was represented by a. flavus, a. niger, a. foetidus and a. tungius, with the former species being the most dominant. the other species were cladosporium herbaru ... | 2005 | 16209108 |
| production of gfp and glucoamylase by recombinant aspergillus niger: effects of fermentation conditions on fungal morphology and protein secretion. | the effect of filamentous fungal morphology on heterologous protein secretion was investigated using the recombinant aspergillus niger strain ab4.1[pgpdaglagfp], which contained the gene coded for the gla-gfp (glucoamylase-green fluorescence protein) fusion protein. three culturing systems were studied to develop different morphological forms of the fungus. free-cell cultures in conventional stirred-tank bioreactors grew in pellet form with various sizes depending on culturing conditions. cells ... | 2005 | 16209542 |
| fungal solid state culture of palm kernel cake. | palm kernel cake (pkc), an agro-industrial by-product used extensively in the animal feed industry, has limited use in fish feeds due to its high fiber and low protein contents. in this study, pkc was processed under solid state culture conditions with five fungal strains and the effect of this fungal culturing on the amino acid, fatty acid, cellulose and hemicellulose fractions was evaluated. fungal strains used were sclerotium rolfsii, trichoderma harzianum, trichoderma longiobrachiatum, trich ... | 2006 | 16216731 |
| myceliatomas (aspergillus niger) in the renal collecting system and ureters. | | 2005 | 16217384 |
| multi-objective optimization in aspergillus niger fermentation for selective product enhancement. | a multi-objective optimization formulation that reflects the multi-substrate optimization in a multi-product fermentation is proposed in this work. this formulation includes the application of epsilon-constraint to generate the trade-off solution for the enhancement of one selective product in a multi-product fermentation, with simultaneous minimization of the other product within a threshold limit. the formulation has been applied to the fed-batch fermentation of aspergillus niger that produces ... | 2005 | 16217656 |
| conidia of black aspergilli as new biological adsorbents for ochratoxin a in grape juices and musts. | biological removal of ochratoxin a (ota) by living and heat-treated dead conidia of black aspergillus isolates representing the species aspergillus niger, aspergillus carbonarius, and aspergillus japonicus in synthetic and natural grape juices was found to be a two-stage phenomenon. several lines of evidence suggest that the first observed stage was passive, metabolism was not required, and ota adsorption on conidia of black aspergilli could be involved. this removal was fast, without delay just ... | 2005 | 16218668 |
| citric acid fermentation by magnetic drum contactor: use of methanol and ethanol for higher production. | use of methanol or ethanol in the magneto-biotechnological technique was found to be enhance citric acid production in a magnetic drum contactor operation with aspergillus niger aj 117173. continuous and repeated-batch fermentations were conducted with the addition of 2% ethanol. continuous fermentation for 50 d gave a better citric acid yield (85%) and average productivity (3.8 g.l(-1).d(-1)) than repeated-batch fermentation over 60 d (65%, 2.3 g.l(-1).d(-1)). | 1999 | 16232489 |
| subsite affinities of beta-glucosidase from aspergillus sojae on various xylooligosaccharides. | the subsite affinities of beta-glucosidase (ec 3.2.1.21) with high beta-xylosidase activity from aspergillus sojae on various xylooligosaccharides (degree of polymerization: n = 2-6) were investigated by steady-state kinetic analysis. the molecular activity (k0) value of the enzyme for xylobiose was not markedly different from those of other substrates (n = 3-6). the arrangement of the subsite affinities (a(i), i = 1-6) was evaluated; a1 = 2.93 kcal/mol, a2 = 3.67 kcal/mol, a3 = 0.64 kcal/mol, a ... | 1999 | 16232520 |
| transglycosylation activity of the endo-beta-1,4-glucanase from aspergillus niger ifo31125 and its application. | endo-beta-1,4-glucanase from aspergillus niger was found to have an endo-type transglycosylation activity. the enzyme effectively transferred cellooligosaccharide residues to various 1-alkanols in the presence of cellopentaose as the oligosaccharide donor. by incubating the enzyme with 1-octanol and cellopentaose in the presence of acetonitrile, 1-octyl-cellotrioside was synthesized. the product was isolated by silica gel column chromatography and analyzed by mass spectrometry. p-np-beta-glc, p- ... | 1999 | 16232521 |
| cloning and sequencing of the chromosomal dna and cdna encoding the mitochondrial citrate synthase of aspergillus niger wu-2223l. | the complementary dna (cdna) and chromosomal dna encoding the citrate synthase (ec 4.1.3.7) gene (cit1) of aspergillus niger wu-2223l, a citric acid-producing strain, were cloned. synthetic oligonucleotide primers were designed according to the amino acid sequences of already known eukaryotic citrate synthases and the codon bias of a. niger genes. the 920-bp dna fragment was amplified by polymerase chain reaction with these primers using chromosomal dna of wu-2223l as a template, and was employe ... | 1999 | 16232605 |
| biocatalytic production of chiral epichlorohydrin in organic solvents. | enantioselective hydrolysis of racemic epichlorohydrin was accomplished for the production of enantiopure epichlorohydrin using the whole cells of an isolated aspergillus niger spps. to overcome the spontaneous chemical degradation of epichlorohydrin that occurs in aqueous buffer, organic solvents were employed in the reaction medium. the enantioselectivity was highly dependent on the solvent structure, water content of the medium, and the initial epichlorohydrin concentration. (s)-epichlorohydr ... | 1999 | 16232624 |
| transcriptional analysis of two endoinulinase genes inua and inub in aspergillus niger and nucleotide sequences of their promoter regions. | aspergillus niger 12 contained two copies of endoinulinase genes (inua and inub) in the genome (k. ohta et al., biosci. biotechnol. biochem., 62, 1731-1738, 1998). the inua- and inub-specific dna probes were constructed according to the respective 3'-noncoding sequences that diverged from each other. poly(a)+ rna was prepared from mycelia grown on inulin, fructose, or glucose in submerged culture. three endoinulinase cdna sequences that corresponded to the coding regions and their 5'- and 3'-fla ... | 1999 | 16232670 |
| cloning, sequencing and expression of an alpha-l-arabinofuranosidase from aspergillus sojae. | the arabinofuranosidase gene was cloned from the cdna of aspergillus sojae. it was found to contain an open reading frame composed of 984 base pairs (bp) and to encode 328 amino acid residues (aa). the cdna sequence suggested that the mature enzyme is preceded by a 26-aa signal sequence and the molecular mass was predicted to be 32,749 da. the a. sojae arabinofuranosidase consists of a single catalytic domain; it does not have a specific substrate-binding domain such as the xylan-binding domain ... | 2000 | 16232740 |
| hydrolysis of beta-glucosyl ester linkage of p-hydroxybenzoyl beta-d-glucose, a chemically synthesized glucoside, by beta-glucosidases. | to investigate the hydrolysis of glucosyl esters by beta-glucosidase, p-hydroxybenzoyl beta-d-glucose (phbg) was chemically synthesized. the hydrolytic activity of some beta-glucosidases for phbg was compared to that for p-nitrophenyl beta-glucoside (pnpg). the clavibacter michiganense and flavobacterium johnsonae enzymes could hydrolyze phbg and steviol glycosides which are natural glucosyl esters. the commercial beta-glucosidase originating from caldocellum saccharolyticum also hydrolyzes phbg ... | 2000 | 16232920 |
| preparation of detergent-lipase complexes utilizing water-soluble amphiphiles in single aqueous phase and catalysis of transesterifications in homogeneous organic solvents. | a novel method of preparing detergent-enzyme complexes that can be employed in organic media was developed utilizing newly synthesized water-soluble nonionic gemini-type detergents, n,n-bis(3-d-gluconamidopropyl)-3-(dialkyl-l-glutamatecarbonyl)propanamides (big2cnca: n = 10,12,14,16,18) and n,n-bis(3-d-lactonamidopropyl)-3-(dialkyl-l-glutamatecarbonyl)propanamides (bil2cnca: n = 16,18), and nonionic twin-headed detergents, n,n-bis(3-d-gluconamidopropyl)alkanamides (big1cn: n = 12,14,16,18,delta9 ... | 2000 | 16232923 |
| a second pectin lyase gene (pel2) from aspergillus oryzae kbn616: its sequence analysis and overexpression, and characterization of the gene products. | a second pectin lyase gene, designated pel2, was isolated from a shoyu koji mold aspergillus oryzae kbn616 and characterized. the structural gene comprised 1306 bp with three introns. the orf encoded 375 amino acids with a signal peptide of 19 amino acids. the deduced amino acid sequence showed high similarity to those of a. oryzae pel1, aspergillus niger pectin lyases and glomerella cingulata pn1a. the pel2 gene was overexpressed under the control of the promoter of the a. oryzae tef1 gene for ... | 2001 | 16233008 |
| cloning of a gene encoding a highly stable endo-beta-1,4-glucanase from aspergillus niger and its expression in yeast. | a gene encoding an endo-beta-1,4-glucanase, which is highly resistant to high temperature, protease and surfactant treatment, was isolated from aspergillus niger ifo31125 and designated as eng1. the deduced amino acid sequence encoded by eng1 showed high homology with the sequence of a not-well-characterized cellulase encoded by eglb which has not yet been shown to be a stable enzyme. to confirm the sequence of the gene encoding the highly stable endo-beta-1,4-glucanase, the cloned gene was expr ... | 2001 | 16233124 |
| cloning and expression of aspergillus niger icda gene encoding mitochondrial nadp+-specific isocitrate dehydrogenase. | the complementary dna (cdna) and chromosomal dna (icda) encoding the nadp+-specific isocitrate dehydrogenase (ec 1.1.1.42) of aspergillus niger wu-2223l, a citric acid-producing strain, were cloned. two cdna clones (cdna-1, 2.0 kb; cdna-2, 1.5 kb) were obtained and sequenced, and an orf of 1494 base pairs (bp) encoding a protein of 498 amino acids (aa) was identified in cdna-1. the predicted amino acid sequence showed 73% and 67% sequence identities with those of the mitochondrial nadp+-icdhs fr ... | 2002 | 16233178 |
| biosynthesis of p-anisaldehyde by the white-rot basidiomycete pleurotus ostreatus. | the white-rot basidiomycete pleurotus ostreatus produced sweet flavor compounds on a liquid medium. the major and minor compounds identified by gc-ms analysis were p-anisaldehyde (4-methoxybenzaldehyde) and 3-chloro-p-anisaldehyde (3-chloro-4-methoxybenzaldehyde), respectively. p-anisaldehyde was only produced under static culture conditions. differences in the type and quantity of flavor compounds produced among wild strains of p. ostreatus were observed. aryl alcohol oxidase and manganese pero ... | 2002 | 16233188 |
| cloning and transcription analysis of the aspergillus aculeatus no. f-50 endoglucanase 2 (cmc2) gene. | the cmc2 gene, coding for an endoglucanase 2 (cmc2) of aspergillus aculeatus, was cloned using both genomic and cdna libraries, and sequenced. the gene consists of 1230 bp encoding a protein of 410 amino acid residues with a molecular mass of 43,697 da. the cmc2, composed of an n-terminal catalytic domain belonging to the family 5 of glycosyl hydrolases and a c-terminal cellulose-binding domain (cbd) belonging to the family i of cbds, showed identity with other fungal endoglucanases, particularl ... | 2002 | 16233338 |
| role of two alpha-l-arabinofuranosidases in arabinoxylan degradation and characteristics of the encoding genes from shochu koji molds, aspergillus kawachii and aspergillus awamori. | two different alpha-l-arabinofuranosidases from aspergillus kawachii were purified and characterized. the two enzymes acted synergically with xylanase in the degradation of arabinoxylan and resulted in an increase in the amount of ferulic acid release by feruloyl esterase. both enzymes were acidophilic and acid stable enzymes which had an optimum ph of 4.0 and were stable at ph 3.0-7.0. the general properties of the enzymes including ph optima and ph stability were similar to those of aspergillu ... | 2003 | 16233515 |
| molecular cloning and characterization of an exoinulinase gene from aspergillus niger strain 12 and its expression in pichia pastoris. | a genomic dna segment and cdnas encoding an extracellular exoinulinase from aspergillus niger strain 12 were cloned and sequenced. southern blot analysis indicated that the exoinulinase gene (inue) was present as a single copy in the genome. an open reading frame of 1611 by was interrupted by a single intron of 60 bp, and encoded a 19-amino acid signal peptide and a 518-amino acid mature protein. the mature protein contained a single cys residue and nine potential n-linked glycosylation sites. t ... | 2003 | 16233531 |
| a novel amine oxidase-encoding gene from aspergillus oryzae. | we cloned a novel gene (aoxa) encoding amine oxidase (aox) from aspergillus oryzae. one cdna clone showing extreme homology to the aox-encoding genes was found in an expressed sequence tag (est) library of a. oryzae. molecular analysis revealed that the aoxa carried four exons interrupted by three introns and had an open reading frame encoding 672 amino acid residues. the deduced amino acid sequence showed about 83.5% identity to the aspergillus niger ao-i. the strictly conserved residues for co ... | 2004 | 16233720 |
| functional analysis of tunicamycin-inducible gene a polypeptide from aspergillus niger. | tunicamycin-inducible gene a polypeptide (tiga) is a member of the protein disulfide isomerase (pdi) family and is suggested to facilitate the folding of nascent polypeptides. the functional properties of tiga were investigated here. tiga acted as an isomerase, catalyzing the refolding of denatured and reduced ribonuclease a. tiga also exhibited chaperone activity in the refolding of denatured prochymosin but not in the refolding of glyceraldehyde 3-phosphate dehydrogenase (gapdh), indicating th ... | 2005 | 16234854 |
| olive-mill wastewaters: a promising substrate for microbial lipase production. | the present study investigated the valorization of olive-mill wastewater (omw) by its use as a possible growth medium for the microbial production of extra-cellular lipase. to this end, strains of geotrichum candidum (nrrl y-552 and y-553), rhizopus arrhizus (nrrl 2286 and isrim 383), rhizopus oryzae (nrrl 6431), aspergillus oryzae (nrrl 1988 and 495), aspergillus niger (nrrl 334), candida cylindracea (nrrl y-17506) and penicillium citrinum (nrrl 1841 and 3754, isrim 118) were screened. all stra ... | 2006 | 16236495 |
| hyphal differentiation in the exploring mycelium of aspergillus niger. | mycelial fungi play a central role in element cycling in nature by degrading dead organic material such as wood. fungal colonization of a substrate starts with the invasion of exploring hyphae. these hyphae secrete enzymes that convert the organic material into small molecules that can be taken up by the fungus to serve as nutrients. using green fluorescent protein (gfp) as a reporter, we show for the first time that exploring hyphae of aspergillus niger differentiate with respect to enzyme secr ... | 2005 | 16238620 |
| modulation of water activity on fungicide effect on aspergillus niger growth in sabouraud dextrose agar medium*. | to examine whether water activity (aw) in combination with low concentration of fungicides can be used to effectively control aspergillus niger van tieghem growth in cultural medium, the sabouraud dextrose agar (sda). the data would be used as baseline information for reducing a. niger contamination in insect artificial diets. | 2005 | 16238647 |
| chemical composition and biological activities of essential oil from the leaves of sesuvium portulacastrum. | sesuvium portulacastrum has long been used as a remedy for fever and scurvy. hydrodistillation was used to extract the essential oil from the fresh leaves of sesuvium portulacastrum. the essential oil yield obtained was 0.15%. using gc-ms analysis, alpha-pinene, camphene, beta-pinene, alpha-terpinene, o-cymene, limonene, 1,8-cineole, alpha-terpinene, bornyl acetate, tridecane, trans-caryophyllene and alpha-humulene were identified. the hole plate diffusion method was used for antibacterial testi ... | 2006 | 16243465 |
| purification and physico-chemical characterisation of genetically modified phytases expressed in aspergillus awamori. | two heterologous phytases from aspergillus awamori and aspergillus fumigatus obtained from submerged cultures of genetically modified fungal strains in addition to two commercially available phytase preparations (allzyme and natuphos phytases) were purified to homogeneity using a combination of ultrafiltration, gel filtration and ion exchange. the purified preparations were used in subsequent characterisation studies, in which western immunoblot analysis, ph and temperature optima, thermal stabi ... | 2006 | 16243522 |
| [study on breeding of probiotic aspergillus nigre by n+ implantation and fermentation condition of its mutant]. | using aspergillus niger an01 as original strain, mutated strain aspergillus niger an03 was obtained by n+ implantation. the results showed that activities of acidic protease, cellulase and pectinase of aspergillus niger were raised from 71.6u/g, 141.7u/g and 264.8u/g to 996.5u/g, 940.4u/g and 906.5u/g respectively. characteristics of enzymes production in the mutant aspergillus niger an03 kept stabile by 5 times subculture. in addition, the optimum conditions for enzymes production of aspergillu ... | 2005 | 16245865 |
| influence of mechanical stress and surface interaction on the aggregation of aspergillus niger conidia. | productivity of fungal cultures is closely linked with their morphologic development. morphogenesis of coagulating filamentous fungi, like aspergillus niger, starts with aggregation of conidia, also denominated as spores. several parameters are presumed to control this event, but little is known about their mode of action. rational process optimization requires models that mirror the underlying reaction mechanisms. an approach in this regard is suggested and supported by experimental data. aggre ... | 2005 | 16255057 |
| structural characterization of novel chitin-binding lectins from the genus artocarpus and their antifungal activity. | two novel chitin-binding lectins from seeds of artocarpus genus were described in this paper, one from a. integrifolia (jackfruit) and one from a. incisa (breadfruit). they were purified from saline crude extract of seeds using affinity chromatography on chitin column, size-exclusion chromatography and reverse-phase chromatography on the c-18 column. both are 14 kda proteins, made up of 3 chains linked by disulfide bonds. the partial amino acid sequences of the two lectins showed they are homolo ... | 2006 | 16257591 |
| micromechanical cantilever array sensors for selective fungal immobilization and fast growth detection. | we demonstrate the use of micromechanical cantilever arrays for selective immobilization and fast quantitative detection of vital fungal spores. micro-fabricated uncoated as well as gold-coated silicon cantilevers were functionalized with concanavalin a, fibronectin or immunoglobulin g. in our experiments two major morphological fungal forms were used--the mycelial form aspergillus niger and the unicellular yeast form saccharomyces cerevisiae, as models to explore a new method for growth detecti ... | 2005 | 16257652 |
| production of pectinase from deseeded sunflower head by aspergillus niger in submerged and solid-state conditions. | studies were carried out on the production of pectinases using deseeded sunflower head by aspergillus niger dmf 27 and dmf 45 in submerged fermentation (smf) and solid-state fermentation (ssf). higher titres of endo- and exo-pectinases were observed when medium was supplemented with carbon (4% glucose for smf and 6% sucrose for ssf) and nitrogen (ammonium sulphate, 0.3% for both smf and ssf) sources. green gram husk proved to be relatively a better supplement to attain higher yield of endo-pecti ... | 2006 | 16263274 |
| the development of 3d-qsar study and recursive partitioning of heterocyclic quinone derivatives with antifungal activity. | it was reported that some 1,4-quinone derivatives such as 6-(n-arylamino)-7-chloro/6,7-bis[s-(aryl)thio]-5,8-quinolinedione and 6-arylthio-/5,6-arylamino-4,7-dioxobenzothiazoles have antifungal effects. to understand the structural basis for antifungal activity and guide in the design of more potent agents, we performed three-dimensional quantitative structure-activity relationship studies for a series of compounds using comparative molecular field analysis (comfa). the mic values of 1,4-quinone ... | 2006 | 16263301 |
| fate and role of ammonium ions during fermentation of citric acid by aspergillus niger. | stoichiometric modeling of the early stages of the citric acid fermentation process by aspergillus niger revealed that ammonium ions combine with a carbon-containing metabolite inside the cell, in a ratio 1:1, to form a nitrogen compound which is then excreted by the mycelium. high-performance liquid chromatography analysis identified glucosamine as the product of the relationship between glucose and ammonium during the early stages of the citric acid fermentation process. slightly acidic intern ... | 2005 | 16269757 |
| transformation of ginsenosides rb2 and rc from panax ginseng by food microorganisms. | protopanaxadiol ginsenosides rb2 and rc were transformed using cell-free extracts from various edible food microorganisms and then analyzed by tlc and hplc. rb2 and rc were transformed into compound k via rd and f2 by bifidobacterium sp. int57 and bifidobacterium sp. sj32. lactobacillus delbrueckii transformed rb2 and rc into ginsenoside rh2. bifidobacterium sp. sh5 transformed rb2 and rc into f2. aspergillus niger transformed rb2 into compound k via compound o and compound y, whereas it transfo ... | 2005 | 16272697 |
| biotransformation of citrus aromatics nootkatone and valencene by microorganisms. | biotransformations of the sesquiterpene ketone nootkatone from the crude drug alpiniae fructus and grapefruit oil, and the sesquiterpene hydrocarbon valencene from valencia orange oil were carried out with microorganisms such as aspergillus niger, botryosphaeria dothidea, and fusarium culmorum to afford structurally interesting metabolites. their stereostructures were established by a combination of high-resolution nmr spectral and x-ray crystallographic analysis and chemical reaction. metabolic ... | 2005 | 16272725 |
| trp-262 is a key residue for the hydrolytic and transglucosidic reactivity of the aspergillus niger family 3 beta-glucosidase: substitution results in enzymes with mainly transglucosidic activity. | trp-262 of the aspergillus niger family 3 beta-glucosidase is shown in this report to be a key residue for determining the ratio of this enzyme's hydrolytic and transglucosidic activities. tlc showed that when cellobiose was both the substrate and the acceptor, beta-glucosidases with substitutions (phe, ala, leu, and cys) for trp-262 formed very high amounts of transglucosidic adducts. when pnpglc was the substrate and the acceptor of the substituted beta-glucosidases, only transglucosidic adduc ... | 2005 | 16274659 |
| his374 of wheat endoxylanase inhibitor taxi-i stabilizes complex formation with glycoside hydrolase family 11 endoxylanases. | wheat endoxylanase inhibitor taxi-i inhibits microbial glycoside hydrolase family 11 endoxylanases. crystallographic data of an aspergillus niger endoxylanase-taxi-i complex showed his374 of taxi-i to be a key residue in endoxylanase inhibition. its role in enzyme-inhibitor interaction was further investigated by site-directed mutagenesis of his374 into alanine, glutamine or lysine. binding kinetics and affinities of the molecular interactions between a. niger, bacillus subtilis, trichoderma lon ... | 2005 | 16279951 |
| antifungal activity of some actinomycetes isolated from various habitats. | a total of 287 actinomycetes were isolated from 79 samples collected from five different habitats i.e., cultivated field soil (cfs), garden soil (gs), compost (cm), decaying organic matter (dom) and stored agricultural products (sap) of different localities of sagar, madhya pradesh (23 degrees 50 degrees n latitude and 78 degrees 40 degrees e longitude). these were screened for antagonistic activity against candida albicans, aspergillus niger, microsporum gypseun and trichophyton sp. using 'cros ... | 2003 | 16281822 |
| screening of actinomycetes isolated from soil samples of ajmer, rajasthan for antimicrobial activity. | several strains of actinomycetes were isolated from soil samples collected from various localities ofajmer district. these isolates were tested for their antagonistic proerties against few test organisms viz. bacillus subtilis, escherichia coli, pseudomonas aeruginosa, staphylococcus sp., saccharomyces cerevisiae and aspergillus niger. some of these actinomycete strains exhibited antimicrobial activity against bacteria, no antifungal activity was observed. six such isolates were selected for det ... | 2003 | 16281825 |
| influence of galactomannans with different molecular weights on the gelation of whey proteins at neutral ph. | the effect of locust bean gum, a galactomannan, with different molecular weights on the microstructure and viscoelastic properties of heat-induced whey protein gels has been studied using confocal laser scanning microscopy and small-deformation rheology. the results obtained clearly showed that differences in the molecular weight of the polysaccharide have a significant influence on the gel microstructure. homogeneous mixtures and phase-separated systems, with dispersed droplet and bicontinuous ... | 2005 | 16283758 |
| isolation and sequencing of a new glucoamylase gene from an aspergillus niger aggregate strain (dsm 823) molecularly classified as aspergillus tubingensis. | based on morphological characteristics the taxa included in the aspergillus aggregate can hardly be differentiated. for that reason the phylogeny of this genus was revised several times as different criteria, from morphological to later molecular, were used. we found, comparing nucleotide sequences of the its-region, that the strain aspergillus niger (dsm 823) which is claimed to be identical to the strains atcc 10577, imi 027809, nctc 7193 and nrrl 2322 can be molecularly classified as aspergil ... | 2005 | 16284933 |
| synthesis and antimicrobial activity of n-alkyl and n-aryl piperazine derivatives. | a series of substituted piperazine derivatives have been synthesized and tested for antimicrobial activity. the antibacterial activity was tested against staphylococcus aureus (mtccb 737), pseudomonas aeruginosa (mtccb 741), streptomyces epidermidis (mtccb 1824) and escherichia coli (mtccb 1652), and antifungal activity against aspergillus fumigatus, aspergillus flavus and aspergillus niger. all synthesized compounds showed significant activity against bacterial strains but were found to be less ... | 2006 | 16289939 |
| synergy between xylanases from glycoside hydrolase family 10 and family 11 and a feruloyl esterase in the release of phenolic acids from cereal arabinoxylan. | the bioconversion of waste residues (by-products) from cereal processing industries requires the cooperation of enzymes able to degrade xylanolytic and cellulosic material. the type a feruloyl esterase from aspergillus niger, anfaea, works synergistically with (1-->4)-beta-d-xylopyranosidases (xylanases) to release monomeric and dimeric ferulic acid (fa) from cereal cell wall-derived material. the esterase was more effective with a family 11 xylanase from trichoderma viride in releasing fa and w ... | 2006 | 16292533 |
| mode of action of beta-glucuronidase from aspergillus niger on the sugar chains of arabinogalactan-protein. | a beta-glucuronidase purified from a commercial pectolytic enzyme preparation of aspergillus niger hydrolyzed about half of the 4-o-methyl-glucuronic acid (4-me-glca) residues located at the nonreducing terminals of (1-->6)-linked beta-galactosyl side chains of the carbohydrate portion of a radish arabinogalactan-protein (agp) modified by treatment with fungal alpha-l-arabinosidase. digestion of the alpha-l-arabinosidase-treated agp with exo-beta-(1-->3)-galactanase released, by exo-fission of b ... | 2005 | 16306699 |
| microbial transformation of fraxinellone by aspergillus niger. | microbial transformation of fraxinellone (1) by aspergillus niger (as 3.421) has been carried out. two converted products, dasycarpol (2) and a new compound fraxinigerllone (3) were obtained. their structures have been identified on the basis of spectroscopic methods. dasycarpol shows moderate inhibitory activity on lung cancer cell line a549. | 2005 | 16308202 |
| synthesis and structure of cycloruthenated carbonyl complexes and their emission, redox and biological properties. | an interesting series of mononuclear organoruthenium complexes of formulation [ru(co)(pph3)2(ap-r)] (where ap-r = -h, -cl, -me, -ome, -oet) have been synthesized from the reaction of five 2-(arylazo)phenol ligands with ruthenium(ii) precursor [ruh(cl)(co)(pph3)3] in benzene under reflux. the 2-(arylazo)phenolate ligands behave as dianionic tridentate ligand and are coordinated to ruthenium through c, n and o by dissociation of the phenolic and phenyl proton at the ortho position of the phenyl ri ... | 2006 | 16310253 |
| enzymatic resolution of racemic phenyloxirane by a novel epoxide hydrolase from aspergillus niger sq-6 and its fed-batch fermentation. | a microorganism with the ability to catalyze the resolution of racemic phenyloxirane was isolated and identified as aspergillus niger sq-6. chiral capillary electrophoresis was successfully applied to separate both phenyloxirane and phenylethanediol. the epoxide hydrolase (eh) involved in this resolution process was (r)-stereospecific and constitutively expressed. when whole cells were used during the biotransformation process, the optimum temperature and ph for stereospecific vicinal diol produ ... | 2006 | 16320035 |