| functional homology of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, phosphoglycerate mutase, and 2,3-bisphosphoglycerate mutase. | the bisphosphatase domain of the rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase has been shown to exhibit a structural similarity to yeast phosphoglycerate mutase and human red blood cell 2,3-bisphosphoglycerate mutase including very similar active site sequences with a histidyl residue being involved in phospho group transfer. the liver bifunctional enzyme was found to catalyze the hydrolysis of glycerate 1,3-bisphosphate to glycerate 3-phosphate and inorganic phosphate. the km ... | 1987 | 2824507 |
| noradrenergic mechanisms in age-related cognitive decline. | the alpha-2 agonist, clonidine, improved spatial working memory performance in 13/13 aged rhesus monkeys with documented memory impairments. the clonidine response was blocked by alpha-2, but not alpha-1 antagonists, and appeared to result from actions at postsynaptic alpha-2 receptors in that area of cortex most critical for spatial working memory, the principal sulcal cortex. the data indicate that noradrenergic mechanisms play an important role in the functioning of the frontal association co ... | 1987 | 2824692 |
| replication and packaging of choleraphage phi 149 dna. | the intercellular replication of the circularly permuted dna of choleraphage phi 149 involves a concatemeric dna structure with a size equivalent to six genome lengths. the synthesis of both monomeric and concatemeric dnas during replication of phi 149 occurred in the cytoplasm. the concatemers served as the substrate for the synthesis of mature phage dna, which was eventually packaged by a headful mechanism starting from a unique pac site in the concatemeric dna. packaging of dna into phage hea ... | 1987 | 2824829 |
| [bacteriologic studies of bicarbonate dialysate and suitable initial solutions]. | dialysates for the haemodialysis are produced unsterile and usually contain bacteria. own investigations of bicarbonate dialysate and adequate initial solutions comprised sterility tests, determinations of the germ count and germ tolerance experiments. only the "acid concentrate" was sterile. in the other solutions corynebacteria, acinetobacter and pseudomonas bacteria dominated as typical water germs. in the fresh reverse osmosis water and the bicarbonate dialysate as well as in the recently pr ... | 1987 | 2825447 |
| opioid receptors inhibit the adenylate cyclase in guinea pig cochleas. | the effects of mu- and delta-preferring agonists on adenylate cyclase activity have been investigated in vitro in homogenates of guinea pig cochleas. morphine, leu-enkephalin, d-ala2, n-methyl-phe4, gly-ol5-enkephalin (dago) and d-ser2-leu-enkephalin-thr (dslet) each inhibited the synthesis of cyclic amp. this effect was reversed by naloxone which had a greater affinity in blocking the effect of the mu-preferring agonists (morphine, dago) than in blocking the effect of the delta-preferring agoni ... | 1987 | 2825909 |
| opioid receptors inhibit the adenylate cyclase in guinea pig cochleas. | the effects of mu- and delta-preferring agonists on adenylate cyclase activity have been investigated in vitro in homogenates of guinea pig cochleas. morphine, leu-enkephalin, d-ala2, n-methyl-phe4, gly-ol5-enkephalin (dago) and d-ser2-leu-enkephalin-thr (dslet) each inhibited the synthesis of cyclic amp. this effect was reversed by naloxone which had a greater affinity in blocking the effect of the mu-preferring agonists (morphine, dago) than in blocking the effect of the delta-preferring agoni ... | 1987 | 2825909 |
| chronic administration of sertraline, a selective serotonin uptake inhibitor, decreased the density of beta-adrenergic receptors in rat frontoparietal cortex. | sertraline, a potent and selective inhibitor of serotonin uptake, was chronically administered to laboratory rats. using in vitro receptor autoradiographic techniques, we found that the binding of [3h]dihydroalprenolol ([3h]dha) was reduced in cortex layers iv-vi. results of a saturation experiment indicated that the reduction in cortex layer iv was due to a change in number but not affinity of beta-adrenergic receptors. | 1987 | 2825912 |
| chronic administration of sertraline, a selective serotonin uptake inhibitor, decreased the density of beta-adrenergic receptors in rat frontoparietal cortex. | sertraline, a potent and selective inhibitor of serotonin uptake, was chronically administered to laboratory rats. using in vitro receptor autoradiographic techniques, we found that the binding of [3h]dihydroalprenolol ([3h]dha) was reduced in cortex layers iv-vi. results of a saturation experiment indicated that the reduction in cortex layer iv was due to a change in number but not affinity of beta-adrenergic receptors. | 1987 | 2825912 |
| effects of caffeine and cyclic adenosine 3',5'-monophosphate on adenosine triphosphate-dependent calcium uptake by lysed brain synaptosomes. | the adenosine triphosphate-dependent calcium uptake by endoplasmic reticulum elements of lysed synaptosomes from rat brain cortex was studied. caffeine exhibited a biphasic effect on this calcium uptake activity: concentrations of 1, 2, 5, 10 or 30 mm caffeine stimulated calcium uptake by 62, 111, 73, 88 and 60% respectively, whereas calcium uptake was inhibited by 55% at a 60-mm concentration of caffeine. calcium release from endoplasmic reticulum elements of lysed brain synaptosomes was stimul ... | 1987 | 2825917 |
| dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites. | selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ... | 1987 | 2825926 |
| dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites. | selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ... | 1987 | 2825926 |
| murine cytomegalovirus-pseudomonas synergistic infections: comparison of virulent and attenuated virus. | the synergistic interactions between pseudomonas aeruginosa and virulent or attenuated murine cytomegalovirus (mcmv) were compared in vivo. virulent mcmv challenge at a dose of 5 x 10(5) pfu/mouse intraperitoneally, followed by intranasal superinfection with 5 x 10(6) cfu/mouse of pseudomonas aeruginosa after 48 h resulted in greater than 80% mortality, apparently owing to a failure of pulmonary clearance mechanisms. single infections, or the use of attenuated mcmv in synergistic infections, did ... | 1987 | 2825950 |
| transient outward current prominent in canine ventricular epicardium but not endocardium. | previous studies have denied the presence of a transient outward current (ito) in ventricular myocardium of dog, sheep, and calf. using conventional microelectrode techniques, we provide evidence for a significant contribution of ito to epicardial, but not endocardial, activity of canine ventricular myocardium. the epicardial action potential when compared with that of endocardium shows a smaller phase 0 amplitude, a much more prominent phase 1, and a phase 2 amplitude that is greater than that ... | 1988 | 2826039 |
| transient outward current prominent in canine ventricular epicardium but not endocardium. | previous studies have denied the presence of a transient outward current (ito) in ventricular myocardium of dog, sheep, and calf. using conventional microelectrode techniques, we provide evidence for a significant contribution of ito to epicardial, but not endocardial, activity of canine ventricular myocardium. the epicardial action potential when compared with that of endocardium shows a smaller phase 0 amplitude, a much more prominent phase 1, and a phase 2 amplitude that is greater than that ... | 1988 | 2826039 |
| comparison of solutions for extracting ck, ak, and ld from brain samples. | | 1987 | 2826051 |
| tetanus toxin binds with high affinity to neuroblastoma x glioma hybrid cells ng 108-15 and impairs their stimulated acetylcholine release. | differentiated neuroblastoma x glioma hybrid cells ng 108-15 express on their surface specific binding sites for tetanus toxin. 450 sites/cell with a kd of 2 x 10(-11) m were found under "physiological" conditions of ph and salt concentrations. a hill coefficient of 1.1 indicated noncooperative binding. specific binding of 125i-toxin to its sites could be prevented either by preincubation of the toxin with a neutralizing monoclonal antibody or by pretreatment of the cells with neuraminidase (vib ... | 1987 | 2826418 |
| characterization and restriction analysis of the p sex factor and the cryptic plasmid of vibrio cholerae strain v58. | the p plasmid of vibrio cholerae is a derepressed sex factor restricted to v. cholerae and has been shown to express surface exclusion. we have isolated the plasmids of strain v58 and have found that in addition to p, two further cryptic plasmids are also present. p has a size of 68 kb as determined by both electron microscopy and restriction endonuclease analysis. these other plasmids are 34 and 4.7 kb in size. restriction maps of p and the larger cryptic plasmid have been determined. it has be ... | 1987 | 2827202 |
| [classification of spot-shaped lung changes by texture analysis]. | classification of opacities in pneumoconiosis was accomplished by textural analysis in digitised chest x-rays. a good discrimination was achieved by a set of 10 parameters. these texture measures were computed by algorithms for edge detection, local extremes, difference statistics, the co-occurrence matrix and the power spectrum. the classes of the training set were classified correctly at 99%. a test set comprising additional classes that were not contained in the training set, was classified a ... | 1987 | 2827257 |
| derivation of a monoclonal antibody against the group specific antigen of rotaviruses and its use in a diagnostic enzymatic immunoassay. | | 1986 | 2827408 |
| mucosal antibody response of calves after oral and intrabronchial administration of rotavirus. | | 1987 | 2827448 |
| herpes simplex virus infection of the hand. a profile of 79 cases. | seventy-nine cases of herpes simplex (hsv) infection of the hand were documented by viral culture over a 40-month period. a marked bimodal age distribution was found with 32 cases occurring in adults aged 21 to 30 years and 16 occurring in children less than 10 years old. the infection most commonly occurred on the fingers (69 percent) and thumb (21 percent). all 20 herpes simplex type 2 (hsv-2) isolates came from patients over 20 years old. all of the 13 specimens that were typed after isolatio ... | 1988 | 2827469 |
| l929 cells infected with temperature sensitive mutants of vesicular stomatitis virus: virus replication is necessary for induction of changes in membrane permeability. | infection of l929 murine cells with vesicular stomatitis virus (vsv) results in inhibition of host protein synthesis and appearance of membrane alterations at a time when cells are still actively engaged in viral protein synthesis. vsv temperature-sensitive (ts) mutants have been used to explore the role(s) played by the virus-coded proteins in the genesis of these effects. cells were infected with each of five ts mutants representing the known complementation groups of vsv indiana serotype, and ... | 1987 | 2827608 |
| l929 cells infected with temperature sensitive mutants of vesicular stomatitis virus: virus replication is necessary for induction of changes in membrane permeability. | infection of l929 murine cells with vesicular stomatitis virus (vsv) results in inhibition of host protein synthesis and appearance of membrane alterations at a time when cells are still actively engaged in viral protein synthesis. vsv temperature-sensitive (ts) mutants have been used to explore the role(s) played by the virus-coded proteins in the genesis of these effects. cells were infected with each of five ts mutants representing the known complementation groups of vsv indiana serotype, and ... | 1987 | 2827608 |
| lipid-dependent membrane enzymes. purification to homogeneity and further characterization of diacylglycerol kinase from escherichia coli. | 1. diacylglycerol kinase apoprotein was purified from membranes of escherichia coli k12 by a six-step procedure that included hplc. the proposed assignment of the enzyme to the dgka gene [lightner et al. (1983) j. biol. chem. 258, 10856-10861] could be supported by molecular mass determination (approximately 14 kda), n-terminal sequencing (met-ala-asn), cyanogen bromide fragmentation and amino acid analysis. as predicted, proline was absent. 2. the membrane-associated as well as the butan-1-ol-d ... | 1988 | 2828054 |
| a comparison of pcp-like compounds for nmda antagonism in two in vivo models. | the pcp-like compounds ketamine and dexoxadrol were evaluated in two behavioral test procedures known to be sensitive to competitive n-methyl-d-aspartate (nmda) receptor antagonists. in the nmda-induced convulsion test in mice, ketamine and dexoxadrol blocked convulsant activity only at doses that also induced nonspecific effects of pcp-like behaviors, thereby confounding the interpretation of results. these compounds also blocked nmda-induced discriminative stimuli in rats; however, this effect ... | 1988 | 2828792 |
| inhibition of rat brain adenylate cyclase activity by benzodiazepine through the effects on gi and catalytic proteins. | the effect of benzodiazepines on adenylate cyclase system was examined in rat brain. micromolar concentrations of diazepam inhibited the enzyme activity in synaptic membranes in dose- and time-dependent manners. the inhibitory effect of diazepam was more evident on the enzyme activity in the presence of guanylyl-5'-imidodiphosphate (gppnhp) or naf-alcl3 than on that in the basal state. in the pertussis toxin-treated membranes, the effect of diazepam in the presence of gppnhp or naf-alcl3 was mar ... | 1988 | 2828793 |
| differential effects of benzodiazepines on phospholipid methylation in hippocampus and cerebellum of rats. | to elucidate the relationship between the occupancy of bdz binding sites and phospholipid methylation in brain, we examined phosphatidylethanolamine-n-methyltransferase (pemt) activity in synaptosomes of rat hippocampi and cerebella in the presence of bdz ligands with different modes of action. we found that ro 5-4864, a specific ligand for "peripheral type" receptors, increased pl methylation in hippocampal and cerebellar synaptosomes. this effect was directly related to receptor occupancy, sin ... | 1988 | 2828796 |
| interaction between highly selective mu and delta opioids in vivo at the rat spinal cord. | possible interactions between mu- and delta-receptors in the rat spinal cord were studied using the radiant-heat-induced tail flick response and the highly selective mu- or delta-ligands: [nmephe3,d-pro4] morphiceptin(pl-17) and cyclic[d-pen2,d-pen5]enkephalin(dpdpe). intrathecal infusions of pl-17 (0.5 microgram/hr) for 5 days caused 61- and 6-fold shifts to the right of the dose-response curves for pl-17 and dpdpe, respectively. since pl-17 and dpdpe are highly selective agonists for mu- and d ... | 1986 | 2829017 |
| investigation of opioid receptor binding protein in adult and fetal mouse brain membranes by photoaffinity labeling. | 125i-[d-ala2,p-n3-phe4-met5]enkephalin was used to investigate the opioid receptor from brain membranes of adult (6 months old) and fetal (16 days gestation) mice. the photolabeled membranes were analyzed by sodium dodecyl sulfate gel electrophoresis. a 46,000-da protein was specifically photo-labeled in the adult mouse brain membranes. the photolabeling of this protein was inhibited in the presence of [d-ala2,met5]enkephalin and a peptide ligand specific for the mu-opioid receptor. no specific ... | 1988 | 2829063 |
| [genetic transformation of somatic cells. xv. production of transgenic mice containing the gene for the envelope protein of the human hepatitis b virus]. | transgenic mice containing in somatic (liver) cells a gene encoding hepatitis virus b surface antigen (hbsag) were obtained and analysed. about 10(4)-10(5) copies of circular plasmid psh21 with hbsag gene under the control of the sv40 early promoter were microinjected into the cytoplasm (pronucleus region) of mouse zygotes. 10 of the 24 animals isolated after this treatment contained 1 to 5 copies of the hbsag gene per genome of hepatocytes (7 males and 3 females). the southern analysis of dna f ... | 1987 | 2830691 |
| [genetic transformation of somatic cells. xv. production of transgenic mice containing the gene for the envelope protein of the human hepatitis b virus]. | transgenic mice containing in somatic (liver) cells a gene encoding hepatitis virus b surface antigen (hbsag) were obtained and analysed. about 10(4)-10(5) copies of circular plasmid psh21 with hbsag gene under the control of the sv40 early promoter were microinjected into the cytoplasm (pronucleus region) of mouse zygotes. 10 of the 24 animals isolated after this treatment contained 1 to 5 copies of the hbsag gene per genome of hepatocytes (7 males and 3 females). the southern analysis of dna f ... | 1987 | 2830691 |
| kirsten murine sarcoma virus abolishes interferon gamma-induced class ii but not class i major histocompatibility antigen expression in a murine fibroblast line. | the effect of infecting fibroblasts with kirsten murine sarcoma virus/murine leukemia virus (ki-msv/mlv) on constitutive and ifn-gamma-induced h-2 antigen expression was investigated. the fibroblasts used were two established cell lines (c3h10t1/2 and balb/c3t3) and fresh embryo fibroblasts from c3h mice. class i antigens were expressed constitutively by balb/c3t3; infection with mlv, msv or the two together had little effect on this constitutive expression. class i antigens (h-2k, h-2d) were st ... | 1988 | 2831293 |
| non-cholinergic basal forebrain neurons project to the contralateral basal forebrain in the rat. | following injections of wheat germ agglutinin-conjugated horseradish peroxidase (wga-hrp) or the fluorescent tracer fluoro-gold into the magnocellular preoptic area and the horizontal limb of the diagonal band, retrogradely labelled neurons were found in the homotopic region of the contralateral basal forebrain. labelled fibers apparently arising from these neurons travelled in the stria medullaris and the habenular commissure to terminate in the contralateral basal forebrain. although the neuro ... | 1988 | 2831483 |
| effect of cyclosporin a immunosuppression on primary lymphotropic herpesvirus infection in the guinea pig. | female guinea pigs pretreated for 2 days with cyclosporin a (csa), were then inoculated intranasally (in) or intraperitoneally (ip) with a lymphotropic herpesvirus (gphlv) and followed by 4 additional daily doses of csa. immunosuppressed animals did not show lymphocytosis and virus infectivity titers in their spleen, cervical lymph node and blood mononuclear cells were lower than those of oil-treated controls. while in-inoculated csa-treated animals expressed higher virus infectivity titer in th ... | 1987 | 2832345 |
| cloning and expression of the vibrio cholerae neuraminidase gene nanh in escherichia coli. | a cosmid gene bank of vibrio cholerae 395, classical ogawa, was screened in escherichia coli hb101 for expression of the vibrio neuraminidase (nanase) gene nanh (n-acylneuraminate glycohydrolase). positive clones were identified by their ability to cleave the fluorogenic nanase substrate 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid. seven nanase-positive clones were detected after screening 683 cosmid isolates with a rapid, qualitative plate assay method. the nanh gene was subcloned ... | 1988 | 2832365 |
| characterization and mapping of the agrocinopine-agrocin 84 locus on the nopaline ti plasmid ptic58. | overlapping segments of ptic58 inserted into cosmid vectors were used to characterize the agrocinopine-agrocin 84 locus from the nopaline/agrocinopine a and b agrobacterium tumefaciens strain c58. all of the clones conferring agrocin 84 sensitivity on agrobacteria also conferred uptake of agrocin 84 and agrocinopines a and b. transposon tn3-hoho1 insertion mutations of one such clone were generated that simultaneously abolished agrocin 84 sensitivity and transport of agrocinopines a and b and ag ... | 1988 | 2832379 |
| glucose and carbachol generate 1,2-diacylglycerols by different mechanisms in pancreatic islets. | diacylglycerols (dag) modulate secretory responses by the activation of protein kinase c. early changes in dag formation induced by the muscarinic receptor agonist carbachol were compared to those caused by the nutrient secretagogue glucose in pancreatic islets. turnover rates of dag were investigated in radiolabeling experiments, whereas changes in total mass and fatty acid composition of dag were assessed by gas-liquid chromatography. when islet lipids were labeled to steady state in tissue cu ... | 1988 | 2832445 |
| isolation of a simian virus 40-simian agent 12 recombinant papovavirus that synthesizes a hybrid large tumour antigen. | the simian virus 40 (sv40) mutant dl1055 carries a 55 base pair deletion in the viral early region coding sequences that causes premature termination of large tumour (t) antigen translation resulting in a protein fragment consisting of the amino-terminal 399 residues. the mutation renders the virus defective. we report the characterization of hyb4001, which was isolated as a single plaque following cotransfection of permissive cells with dl1055 dna and the dna of a related papovavirus, simian ag ... | 1988 | 2832530 |
| effects of spiraprilic acid, an angiotensin converting enzyme inhibitor, on large artery compliance in anesthetized dogs. | the present study examined the actions of spiraprilic acid, a new non-sulfhydryl angiotensin converting enzyme (ace) inhibitor upon aortic compliance (ac) in anesthetized dogs. enalaprilic acid was examined for comparative purposes. ac was determined via sonomicrometric determination of the ratio of aortic systolic-diastolic diameter (mm) and arterial pulse pressure (mmhg). one ac unit (acu) equals 10(-3) mm/mmhg. in non-thiazide pretreated animals, spiraprilic acid (1 mg/kg i.v.), caused a sust ... | 1987 | 2833190 |
| reversal by cyclic amp of the urea-induced inhibition of synthesis of a catabolite-repressible enzyme in vibrio cholerae. | low concentrations of urea, which did not inhibit the synthesis of the catabolite nonrepressible enzyme alkaline phosphatase in vibrio cholerae, or markedly affect its overall growth, specifically inhibited the expression of the tryptophanase operon in a temperature-dependent manner. however, in contrast to what is found in escherichia coli, this urea-induced inhibition of tryptophanase synthesis in v. cholerae could be almost completely relieved by exogenously added cyclic amp. the possible mec ... | 1987 | 2833565 |
| a novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in vibrio cholerae requires toxr. | the toxr gene of vibrio cholerae encodes a transmembrane, dna-binding protein that activates transcription of the cholera toxin operon and a gene (tcpa) for the major subunit of a pilus colonization factor. we constructed site-directed insertion mutations in the toxr gene by a novel method employing the chromosomal integration of a mobilizable suicide plasmid containing a portion of the toxr coding sequence. mutants containing these new toxr alleles had an altered outer membrane protein profile, ... | 1988 | 2836362 |
| adp-ribosylation by cholera toxin of membranes derived from brain modifies the interaction of adenylate cyclase with guanine nucleotides and naf. | we have developed a method to adp-ribosylate the stimulatory guanine nucleotide-binding protein of adenylate cyclase (gs) in brain membranes by using cholera toxin. in particular, we used isonicotinic acid hydrazide and 3-acetylpyridine adenine dinucleotide to inhibit the potent nad-glycohydrolase activity of brain membranes, and we used the detergent triton x-100 (at 0.1%) to improve the accessibility of the toxin and guanine nucleotides used for supporting the adp-ribosylation. this method rev ... | 1988 | 2836559 |
| adp-ribosylation by cholera toxin of membranes derived from brain modifies the interaction of adenylate cyclase with guanine nucleotides and naf. | we have developed a method to adp-ribosylate the stimulatory guanine nucleotide-binding protein of adenylate cyclase (gs) in brain membranes by using cholera toxin. in particular, we used isonicotinic acid hydrazide and 3-acetylpyridine adenine dinucleotide to inhibit the potent nad-glycohydrolase activity of brain membranes, and we used the detergent triton x-100 (at 0.1%) to improve the accessibility of the toxin and guanine nucleotides used for supporting the adp-ribosylation. this method rev ... | 1988 | 2836559 |
| treatment of cytomegalovirus infections in renal transplant recipients with 9-(1,3-dihydroxy-2-propoxymethyl) guanine. | | 1988 | 2837848 |
| immune response of chickens inoculated with a recombinant avian leukosis virus. | specific-pathogen-free embryos (18-day incubation) and hatched chicks were inoculated with a recombinant avian leukosis virus (alv) produced by recombinant dna techniques. enzyme-linked immunosorbent assays were used to measure the production of viral-protein-specific antibody and the viral protein, p27, in the serum at 2, 5, 8, 14, and 20 weeks of age. of the inoculated chickens surviving to 20 weeks, 64% produced viral-protein-specific antibodies and 42% transiently produced the viral protein, ... | 1988 | 2838018 |
| mechanism of the epsp synthase catalyzed reaction: evidence for the lack of a covalent carboxyvinyl intermediate in catalysis. | in order to detect covalent reaction intermediates in the 5-enolpyruvylshikimate-3-phosphate (epsp) synthase reaction, we have investigated the interaction of epsp synthase with the reaction product epsp. an exchange of epsp-methylene protons could be demonstrated by incubating epsps with epsp in d2o. since trace amounts of contaminating pi would lead to reversal of epsps reaction and hence methylene proton exchange, we added pyruvate kinase, adp, mg++ and k+. under these conditions, any contami ... | 1988 | 2838023 |
| absence of an endogenous regulator of na+,k+-atpase activity in the tissues of cirrhotic rats. | microsomal na+,k+-atpase isolated from the renal cortex of rats with ccl4-induced cirrhosis (cir) showed a higher specific activity than the enzyme obtained from control rats (cor). kinetic studies showed a lower k0.5 for atp (0.08 +/- 0.03 vs. 0.24 +/- 0.04 mm; p less than 0.05), a lower na+ activation constant (9.6 +/- 1.5 vs. 19.0 +/- 1.7 mm; p less than 0.05), and a higher k+ activation constant (1.2 +/- 0.1 vs. 0.6 +/- 0.1 mm; p less than 0.05) for cir. the optimal ph of the enzyme was 0.5 ... | 1988 | 2838057 |
| human and simian immunodeficiency retroviruses: activation and differential transactivation of gene expression. | new west african human immunodeficiency viruses (hiv-2s) and simian immunodeficiency virus (siv) contain functional transactivator (tat) gene and tat response elements. their long terminal repeats (ltr) and tat genes are more related among themselves than to hiv-1 ltr and tat gene. the viral gene expression of hiv-2 as well as siv can be stimulated by t cell activators, such as mitogens and phorbol esters. hiv-2 and siv display a much broader transactivation response specificity than does hiv-1. ... | 1988 | 2840105 |
| selective inhibition of arachidonate 5-lipoxygenase by novel acetohydroxamic acids: biochemical assessment in vitro and ex vivo. | 1. the chemically novel acetohydroxamic acids, bw a4c, bw a137c and bw a797c, are potent inhibitors of the synthesis of leukotriene b4 (ltb4) from arachidonic acid by human leucocyte homogenates: the concentrations required for 50% inhibition (ic50) were 0.1 microm, 0.8 microm and 0.5 microm respectively. inhibition was less at higher concentrations of arachidonic acid. 2. these compounds also inhibited the synthesis of [14c]-5-hete from [14c]-arachidonic acid and the calcium-dependent synthesis ... | 1988 | 2840160 |
| developmental changes in myosin isoforms from slow and fast latissimus dorsi muscles in the chicken. | in the course of muscle differentiation, changes in fibre-type population and in myosin composition occur. in this work, the expression of native myosin isoforms in developing fast-twitch (posterior latissimus dorsi; pld) and slow-tonic (anterior latissimus dorsi; ald) muscles of the chick was examined using electrophoresis under nondissociating conditions. the major isomyosin of 11-day-old embryonic pld comigrated with the adult fast myosin fm3. two additional components indistinguishable from ... | 1988 | 2840316 |
| a new hamster model for adenoviral vaccination. | both adult and baby hamsters infected intranasally with human adenovirus type 5 exhibited virologic, serologic, and histologic evidence of infection. when 8-day old hamsters were infected with 4 x 10(6) pfu, concentrations of virus up to 2 x 10(6) pfu/animal were detected in the lung, peaking on day 2. the minimum infectious dose was 1 x 10(3) pfu/animal. this model may be useful in studies of conventional and recombinant adenoviral vaccines for humans. | 1988 | 2840876 |
| effect of parathyroid hormone and forskolin on cytoskeletal protein synthesis in cultured mouse osteoblastic cells. | parathyroid hormone (pth) has been shown to cause transient cell shape changes in bone cells. we have examined the effects of parathyroid hormone and forskolin on the organization and expression of cytoskeletal proteins in cultured mouse endosteal osteoblastic cells. analysis of [35s]methionine-labeled cytoskeletal proteins isolated on two-dimensional gel electrophoresis showed that pth treatment (24 h) stimulated the de novo biosynthesis of actin, vimentin and tubulins in confluent cells, where ... | 1988 | 2840972 |
| molecular defects in cytochrome oxidase in mitochondrial diseases. | defects of cytochrome c oxidase (cox) show remarkable clinical, biochemical, and genetic heterogeneity. clinically, there are two main groups of disorders, one dominated by muscle involvement, the other by brain dysfunction. biochemically, the enzyme defect may be confined to one or a few tissues (reflecting the existence of tissue-specific isozymes) or affect all tissues. immunologically reactive enzyme protein is decreased in some forms of cox deficiency but not in others. because cox is encod ... | 1988 | 2841309 |
| molecular defects in cytochrome oxidase in mitochondrial diseases. | defects of cytochrome c oxidase (cox) show remarkable clinical, biochemical, and genetic heterogeneity. clinically, there are two main groups of disorders, one dominated by muscle involvement, the other by brain dysfunction. biochemically, the enzyme defect may be confined to one or a few tissues (reflecting the existence of tissue-specific isozymes) or affect all tissues. immunologically reactive enzyme protein is decreased in some forms of cox deficiency but not in others. because cox is encod ... | 1988 | 2841309 |
| egg shell quality and cholecalciferol metabolism in aged laying hens. | calcium-binding protein d28k (calbindin) synthesis, vitamin d metabolism and shell quality were investigated in young and aged laying hens fed diets containing either cholecalciferol (cc) or its 1-hydroxylated derivatives. duodenal calbindin concentration was similar in the young and in the aged laying hens. exogenous 1-hydroxylated cc derivatives increased duodenal calbindin concentration, regardless of age. shell weight and shell density (mg/cm2) were significantly lower (p less than 0.01) in ... | 1988 | 2841436 |
| effect of ethanol on neuromuscular function in the rat diaphragm preparation. | the effect of ethanol on neuromuscular transmission in the isolated phrenic nerve-diaphragm preparation of the rat was studied by analysing its effect on directly- and indirectly-elicited twitch tension, tetanic and post-tetanic twitch tensions, and on the phenomenon of post-tetanic twitch potentiation (ptp). the results show that ethanol (1.0 x 10(-4)-1.0 m) depresses neuromuscular transmission, by reducing the amplitudes of both directly and indirectly elicited twitch and tetanic contractions. ... | 1988 | 2844197 |
| [relation between the characteristic activity of the beta-adrenergic agonist isoproterenol and the percolation properties of cell membrane]. | variations in the intrinsic activity of 1-isoproterenol and in the percentage of high affinity beta-receptor complexes have been studied under a changes in the macrostructure of reticulocyte plasma membranes. the fluid lipid fraction have been reduced in the membranes for this by phospholipase a2/bsa treatment. it was accompanied by a progressive decrease in the fraction of beta-receptors that are able to form the high affinity complexes with 1-isoproterenol (receptor--regulatory n-protein compl ... | 1988 | 2844324 |
| detection of leukotriene b4 in cardiac tissue and its role in infarct extension through leucocyte migration. | the main left coronary artery of rats was ligated near its origin under light ether anaesthesia and the infarction observed for 48 h. the ischaemic area was determined after an intravenous injection of pontamine sky blue dye 1 h before induction of cardiac arrest with potassium chloride. the unstained area (true ischaemic area) decreased with time to 27.1% of the left ventricle at 48 h, whereas the intensely stained area between the normal and the true ischaemic areas increased with time, sugges ... | 1988 | 2844405 |
| t4 cell activation by immobilized phytohemagglutinin: differential capacity to induce il-2 responsiveness and il-2 production. | soluble mitogens, such as pha induce accessory cell (ac)-dependent t cell proliferation. one function of the ac is to create a stimulatory matrix. therefore, experiments were carried out to determine whether pha immobilized onto microtiter plates could stimulate t cells in the absence of ac. peripheral blood t4 cells were cultured under limiting dilution conditions with either soluble or immobilized pha with or without ril-1 beta, ril-2, r-tnf-alpha, an anti-cd28 mab (9.3), or irradiated ebv-tra ... | 1988 | 2844904 |
| [the diagnosis of infectious mononucleosis in childhood]. | | 1988 | 2845334 |
| heterogeneity of glycine receptors and their messenger rnas in rat brain and spinal cord. | messenger rnas isolated from adult or newborn rat spinal cord were fractionated in a sucrose gradient. the fractions were injected into xenopus oocytes to determine their potencies for expression of glycine receptors (glyrs), which were then examined electrophysiologically. the sedimentation profiles disclosed two classes of glyr mrnas, one heavy and the other light. the adult spinal cord was rich in heavy glyr mrna, whereas the light glyr mrna was more abundant in neonatal spinal cord and in ad ... | 1988 | 2845580 |
| endobronchial hamartoma associated with bronchioloalveolar cell carcinoma. | computed tomographic findings in a 60-year-old man with lobar bronchioloalveolar cell carcinoma distal to endobronchial hamartoma are described. | 1988 | 2846241 |
| infection control measures for human immunodeficiency virus (hiv): universal precautions. | | 1988 | 2846803 |
| txb2: cardiostimulant effect that involves beta-adrenoceptor and na+ + k+-atpase activity. | the biological properties of thromboxane b2 (txb2) on isolated rat heart were studied. its actions were compared with u-46619 a thromboxane a2 mimetic compound and with isoproterenol. txb2 induced a concentration-dependent increase in contractility, that was non-competitively antagonized by propranolol. in addition txb2 inhibited na+ + k+-atpase activity at the same concentrations that influenced the mechanical activity. inhibition of beta-adrenoceptors efficiently blocked the inhibitory action ... | 1988 | 2847200 |
| [effect of bilirubin and serum albumin on the na,k-atpase activity in the synaptosomal membrane]. | the absorption spectrum of visible light, characteristic of the free bilirubin being in the aqueous medium, with a single maximum at 440 nm and with the shoulder in the region of 410-420 nm is transformed into the spectrum with two maxima in the region of 460 and 500 nm, respectively, when the pigment is bound in vitro by the synaptosomal membrane. there are two types of sites for bilirubin binding in the membrane particles, differing in the values of constants of association (ka = 0.6 . 10(5) a ... | 1988 | 2847380 |
| influence of immunomodulatory agents on bovine humoral and cellular immune responses to parenteral inoculation with bovine rotavirus vaccines. | sodium diethyldithiocarbamate (dtc), mycobacterium cell wall extract (mcwe, regressin), killed corynebacterium parvum (c. parvum, immunoregulin) and muramyldipeptide (mdp) were each combined with purified, live bovine rotavirus and inoculated into 3 month-old holstein-friesian calves in order to examine their ability to potentiate specific humoral and cellular immune responses. the vaccinated calves were boosted twice at 3 and 6 weeks after initial vaccine inoculation. the rotavirus was administ ... | 1988 | 2847397 |
| blunted cgmp response to anf in vascular smooth muscle cells of shr. | abnormalities in the coupling of atrial natriuretic factor (anf) receptors with the guanosine 5'-cyclic monophosphate (cgmp) system in vascular smooth muscle cells (vsmcs) may play a role in the pathophysiology of hypertension in the spontaneously hypertensive rat (shr). this concept was examined in cultured, aortic vsmcs (passages 6-10) from shr, wistar-kyoto (wky), and american wistar (wis) rats. quiescent vsmcs of the shr (serum deprived for 24 h) had higher anf receptor density (bmax) and lo ... | 1988 | 2847533 |
| inhibition of porphyrin biosynthesis by exogenous 5-aminolevulinic acid in an aerobic photosynthetic bacterium, erythrobacter sp. och 114. | exogenously administered 5-aminolevulinic acid (ala) inhibited the formation of bacteriochlorophyll a (bchl a) in a dose-dependent manner in the aerobic photosynthetic bacterium, erythrobacter sp. strain och 114, under dark growth conditions. the ala concentration required for half-inhibition after 24-h growth was estimated to be about 3.0 mm. porphyrin and bchl precursors were not found in either the cells or the growth medium. the same inhibition was also observed with cytochrome c formation. ... | 1988 | 2847654 |
| isolation and characterization of a specific receptor for human albumin on a group l streptococcus. | certain group l streptococci demonstrate surface receptors for human albumin. binding of 125i-albumin to group l streptococci could be inhibited by unlabelled albumin preparations from humans, dogs, mice and bovines, but not by albumin from rabbits. the albumin-binding proteins (abp) could be solubilized from the streptococcal surface by hot acid treatment of the bacteria and isolated by affinity chromatography on human-albumin sepharose. abp and specific antisera produced against abp inhibited ... | 1988 | 2848386 |
| transcriptional and translational events during reovirus infection. | this short review focuses on the mechanisms involved in transcription and translation in mouse l cells infected with reoviruses. the viral genomic rna (double-stranded), retained in the inner capsid following removal of the outer capsid of the infecting virion, is transcribed by a viral polymerase. the synthesized viral mrna is blocked at the 5' end by a cap structure similar to the cap structure of cellular mrna but synthesized by the viral enzymes of the inner capsid. this viral mrna is also u ... | 1988 | 2848543 |
| conjugation in saccharomyces cerevisiae. | | 1988 | 2848554 |
| sensitive elisa for the gp120 and gp160 surface glycoproteins of hiv-1. | we have used a panel of polyclonal and monoclonal antibodies against gp120 and gp160, the envelope glycoproteins of human immunodeficiency virus type 1, to create rapid, simple, and sensitive twin-site sandwich elisa specific for gp120 and gp160 or for gp160 alone. these assays can detect 500 cos cells in a population transiently transfected with a construct encoding gp120 and gp160, or 50 pg of recombinant gp160. we estimate that the mean amount of gp120 + gp160 in the transfected population is ... | 1988 | 2848557 |
| the role of diffusible molecules in regulating the cellular differentiation of dictyostelium discoideum. | a central problem in developmental biology is to understand how morphogenetic fields are created and how they act to direct regionalized cellular differentiation. this goal is being pursued in organisms as diverse as moulds, worms, flies, frogs and mice. each organism has evolved its own solution to the challenge of multicellularity but there appear to be common underlying principles and, once pattern formation is fully understood in any system, some general truths seem certain to be revealed. a ... | 1988 | 2848678 |
| enzymatic introduction of a fluorescent sialic acid into oligosaccharide chains of glycoproteins. | aiming at the introduction of a fluorescent sialic acid into glycoconjugates, 5-acetamido-9-(3-fluoresceinylthio-ureido)-3,5,9-trideoxy-2-non ulosonic acid (9-fluoresceinyl-neuac) was synthesized which has an intact carbon chain. a) despite the space-filling substituent at c-9, the fluorescent neuac analogue was activated to the corresponding cmp-glycoside by cmp sialic acid synthase from bovine brain. whereas the km value of the synthase was little affected by the modification (km = 2.1 mm, for ... | 1988 | 2848703 |
| transcriptional regulation of the gene encoding the major surfactant protein (sp-a) in rabbit fetal lung. | the expression of the major protein of rabbit pulmonary surfactant (sp-a), a glycoprotein of mr congruent to 29,000-36,000, is regulated during development and by hormones. in the present study, utilizing a cdna insert complementary to mrna coding for sp-a and nuclear transcription elongation assays, we have investigated the developmental and hormonal regulation of transcription of the sp-a gene in rabbit fetal lung tissue. the relative rates of transcription of sp-a mrna increased as a function ... | 1988 | 2848814 |
| a major outer membrane protein in vibrio cholerae is maltose-inducible. | | 1987 | 2849017 |
| ph-dependent accumulation of the vesicular stomatitis virus glycoprotein at the ends of intact virions. | the electron microscope was employed to examine the effect of ph on the distribution of the vesicular stomatitis virus glycoprotein (vsv g-protein) on the surface of intact virions. both rotary shadowed and negatively stained preparations demonstrated that whereas at neutral ph the glycoprotein was evenly distributed along the virus membrane, at ph 5.5 it was concentrated at the ends giving the overall virion a distinct bilobed or bipolar morphology. negatively stained specimens showed that the ... | 1988 | 2849241 |
| expression of env sequences of the bovine leukemia virus (blv) in the yeast saccharomyces cerevisiae. | dna sequences of the envelope (env) gene of the bovine leukemia virus (blv) were expressed in the yeast saccharomyces cerevisiae. two yeast promoters, the repressible pho5 promoter and the constitutive pgk promoter, were used to construct four expression plasmids comprising either a sequence of the surface antigen gp51 or a (gp51 + gp30) sequence. the expressed heterologous gene products were characterized by western blot analysis and competitive radioimmunoassay. by means of northern blot analy ... | 1988 | 2849260 |
| [the relationship between transport site of hpd and photodamage region of human gastric cancer cells in the cell cycle]. | | 1988 | 2849266 |
| post-epizootic surveys of waterfowl for duck plague (duck virus enteritis). | surviving birds from nine duck plague outbreaks in urban and confined waterfowl were sampled for duck plague (dp) virus and dp antibody during 1979-86. duck plague virus was found in combined oral and cloacal swabs of birds from three outbreaks, and dp-neutralizing antibody was demonstrated in some birds from all nine outbreaks. greater prevalence of dp antibody and higher titers were found in survivors from confined populations than from free-flying urban populations. free-flying waterfowl from ... | 1988 | 2849402 |
| retention enhancement by pre-test beta-endorphin and oxotremorine and its reversal by scopolamine. | the pre-test administration of beta-endorphin (0.05 microgram/mouse), oxotremorine sesquifumarate (5.0 micrograms/mouse), or beta-endorphin plus oxotremorine enhanced retention test performance of a conditioned emotional response in mice. the effects were blocked by scopolamine. scopolamine had no effect of its own. the data suggest that the retention enhancement caused by beta-endorphin may involve the secondary activation of cholinergic mechanisms. | 1988 | 2849408 |
| salience and the effects of cs preexposure on aversion conditioning. | rats (n = 84) received preexposure to distilled water or to one of two differently salient flavors, 5.0% casein or 10.0% sucrose, casein being the more salient. each preexposure group then received aversion conditioning to a 5.0% casein or a 10.0% sucrose cs. aversion effects were reliably more enduring to casein than to sucrose. relative to water-preexposed groups, preexposure to casein attenuated aversion effects to the casein cs reliably less than preexposure to sucrose attenuated aversion ef ... | 1988 | 2849411 |
| plasma from uninephrectomized rats stimulates production of inositol trisphosphates and inositol tetrakisphosphate in renal cortical slices. | metabolism of inositol phosphates in renal cortical slices was investigated in vitro after addition of plasma from uninephrectomized or sham-operated rats. plasma from uninephrectomized rats stimulated production of insp3 (inositol trisphosphate) when obtained within the first 3 h after uninephrectomy. with different amounts of added plasma a graded response of insp3 production was obtained. this effect could be prevented by 0.1 microm-tpa (12-o-tetradecanoylphorbol 13-acetate). when analysis of ... | 1988 | 2849423 |
| properties and developmental regulation of the protein phosphatases in dictyostelium discoideum. | the properties and developmental regulation of the protein phosphatases of dictyostelium discoideum were examined. when crude extracts from vegetative cells were separated on a mono q column (fplc) three protein phosphatase peaks, designated p1, p2 and p3 were found. when aggregation and culmination cells were examined only one protein phosphatase peak was observed. this corresponded to phosphatase p1 of vegetative cells. all three of the vegetative cell phosphatase were inhibited by heparin and ... | 1988 | 2849442 |
| muscarinic receptor coupling to inositol phospholipid metabolism in guinea-pig cerebral cortex, parotid gland and ileal smooth muscle. | inositol phospholipid hydrolysis induced by agonist-stimulation of muscarinic receptors has been examined in slices of guinea-pig cerebral cortex, parotid gland and ileal smooth muscle. an assay measuring 3h-inositol phosphate formation from prelabelled lipids in the presence of licl, allowed marked stimulation by agonists to be followed. the pd2-value of carbachol differed markedly, between tissues being more than 10-fold lower in cerebral cortex than in parotid gland. the partial agonist oxotr ... | 1988 | 2849446 |
| a new class of free radical scavengers reducing adriamycin mitochondrial toxicity. | beef heart mitochondria were incubated with adm and nadh. an adriamycin semiquinone radical was detected using esr spectroscopy. the semiquinone radical production rate is decreased upon addition of a scavenger (ad 20) in the reaction medium. nmri mice were treated with ad 20 (70 mg/kg, i.p.) 15 min prior adm injection (20 mg/kg, i.p.) or with adm alone. heart mitochondria were isolated 48 hr later. the enzymatic activities of complex i-iii and complex iv of the mitochondrial respiratory chain w ... | 1988 | 2849451 |
| kininase ii from human seminal plasma. isolation and properties. | a rapid and highly efficient procedure for purification of kininase ii from human seminal plasma is described. after ultra centrifugation, the enzyme was purified by gel filtration on sepharose 6b cl and ion exchange chromatography followed by affinity chromatography of edta-inhibited enzyme on bradykinin-sepharose. the enzyme was specifically inhibited by captopril and bpp9a but not by phosphoramidon. page in the presence of sodium dodecyl sulfate under reducing conditions resulted in two major ... | 1988 | 2849459 |
| [immunologic diagnosis of cytomegalovirus infections in patients after kidney transplantation]. | | 1988 | 2849513 |
| avian nephroblastomas induced by a retrovirus (mav-2) lacking oncogene. ii. search for common sites of proviral integration in tumour dna. | to demonstrate the existence of a common site of integration in independent tumour clones, restriction mapping of the vicinity of integrated mav-2 proviruses in nephroblastoma dna was performed, using southern hybridization with an mav-2 specific probe u3(pat). the results have shown that (1) nephroblastomas are of semiclonal origin. (2) nephroblastoma cells contain an average of 5 clonally located integrated proviruses per diploid genome; they do not contain any detectable amount of non-integra ... | 1988 | 2849568 |
| suppression of sv40-promoted gene expression by differentiation of preadipose cells. | when a plasmid bearing the chloramphenicol acetyltransferase (cat) gene under the control of an sv40 early promoter is introduced into preadipose or adipose cells of line 3t3-f442a, the promoter directs high levels of transient expression of cat. however, when the plasmid is introduced into preadipose cells and the cells are then allowed to differentiate into adipose cells, the expression of the cat gene is suppressed. in this process, the plasmid is not changed detectably in amount, topology, o ... | 1988 | 2849579 |
| effect of na+/h+ exchange inhibitors on agonist-induced contraction of rat aorta. | a number of vasoconstrictor agonists activate the na+/h+ antiport system in vascular smooth muscle, leading to alkalinization of the cytosol and influx of na+. it is believed that agonist-induced na+/h+ exchange may play an important role in contraction. we have evaluated this hypothesis by determining the effect of inhibition of na+/h+ exchange on phenylephrine (pe)-induced contraction of rat aorta. preincubation of rat aorta with the na+/h+ exchange inhibitor amiloride (0.5 mm) inhibited subse ... | 1988 | 2849662 |
| protein kinase c activities are increased in rat thyroid epithelial cells expressing v-ras genes. | both cytoplasmic and membrane-bound protein kinase c activities are increased in: harvey-sarcoma virus, infected thyroid epithelial cells. the cytoplasmic kinase c increase is found in the chromatographic fraction eluted at lower salt concentration (100 mm nacl-s100), while the more acidic protein fraction eluted at higher salt concentration (35 mm nacl-s350) is virtually absent. although the cytoplasmic s100 fraction from the control and ras-virus infected cells display a comparable pbt2 bindin ... | 1988 | 2849929 |
| calcium-permeable channels in vascular smooth muscle: voltage-activated, receptor-operated, and leak channels. | | 1987 | 2850612 |
| epidermal growth factor-stimulated dna synthesis requires an influx of extracellular calcium. | the dependency of normal cell proliferation on adequate extracellular ca2+ levels was further investigated by determining the role of ca2+ influx in epidermal growth factor (egf)-induced rat liver epithelial (t51b) cell dna synthesis. fura-2-loaded t51b cells responded with an increase in [ca2+]i to egf (5-50 ng/ml) that was blocked by low (25 microm) extracellular ca2+ or by pretreatment with 50 microm la3+ to inhibit plasma membrane ca2+ flux. confluent t51b cells treated for 24 h with egf (0. ... | 1988 | 2851595 |
| glomus tumor of the trachea. immunohistochemical and electron microscopic studies. | glomus tumor of the trachea which developed in a 51-year-old man was reported. he experienced frequent episodes of respiratory infections and hemoptosis for 9 months. bronchofiberscopic study demonstrated a polypoid tumor in the upper trachea. local resection of the tumor was performed and it arised from the membranous portion of the third and fourth tracheal rings. histologically, the tumor was composed of medium-sized rounded cells, resembling carcinoid tumor. desmin and myosin immunoreactivit ... | 1988 | 2851778 |
| [changes in the cyclic nucleotide system of t-lymphocytes in experimental tuberculosis in animals]. | | 1988 | 2851783 |
| phorbol ester and dibutyryl cyclic amp reduce content and efflux of taurine in primary cerebellar astrocytes in culture. | in 16-18 days in vitro (div) primary astrocyte cultures prepared from 7- to 9-day-old rats, 48 h exposure to 12,13-phorbol dibutyrate (pdbu) (1 microm) or dibutyryl camp (dbcamp) (1 mm) reduced cellular taurine content, and both basal and 50 mm k+-evoked taurine efflux, but did not alter cellular glutamate or total protein content. decreases in cellular taurine content first became apparent between 1 and 6 h and were maximal after 24 h. treatment also rapidly altered astrocyte morphology to a mo ... | 1988 | 2852322 |
| a novel peptide vasoconstrictor, endothelin, is produced by vascular endothelium and modulates smooth muscle ca2+ channels. | since the discovery of endothelium-dependent vasodilation, vascular endothelium has been recognized as an important functional unit contributing to the regulation of vascular tonus. recent purification, structure determination and molecular cloning of a novel peptidergic vasoconstrictor, endothelin, from the culture supernatant of porcine aortic endothelial cells has further substantiated this concept. starting from a large quantity of serum-fee endothelial cell-conditioned medium, endothelin wa ... | 1988 | 2853725 |
| phenyl-t-butylnitrone, a free radical spin-trapping agent, inhibits acetylcholine-induced relaxation and cyclic gmp accumulation in rat thoracic aorta. | | 1988 | 2854109 |
| establishment and characterization of an epstein-barr virus negative b-cell lymphoma cell line and successful heterotransplantation. | an ebv-negative b-cell lymphoma cell line designated as hbl-1 was established from the pleural effusion of a patient with malignant lymphoma, diffuse, large cell. surface marker studies revealed that hbl-1 cell possessed the same phenotypes of a mature b-cell as the original lymphoma cell from pleural effusion. hbl-1 closely resembled morphologically to the original lymphoma cells observed in the lymph node biopsy and pleural effusion. chromosome analysis revealed that hbl-1 cells showed chromos ... | 1988 | 2854303 |