| opening remarks of president fernando e. agrait on the occasion of the 50th anniversary of the cayo santiago macaque colony. | | 1989 | 2780954 |
| skin test responsiveness to a series of new tuberculins of children living in three vietnamese cities. | a skin test survey was conducted among 1035 children aged 7-19 years living in three cities in vietnam. fifteen new tuberculins, including leprosin-a, were applied; an induration of 2 mm diameter or more was considered positive. compared to some other tropical countries, low levels of sensitisation were recorded and remarkable regional differences were found. positivity to any tuberculins (pooled data) among non-bcg-vaccinated children was significantly lower in hanoi (13.1%) and hochiminh-city ... | 1989 | 2781608 |
| growth of staphylococcus aureus and synthesis of enterotoxins in home-made yoghurt. | staphylococcus aureus strains fri-100, s6, fri-137 and fri 472 were inoculated into milk to study growth and enterotoxin production in home-made yogurts. the yogurt used as starter was progressively weakened by successive inoculations (up to four) in milk to prepare other yogurts in order to study the ability of yogurt microflora to inhibit staphylococci. after elaboration, yogurts were stored at 4 degrees c, 22 degrees c, and 37 degrees c for a maximum of 21 days. periodically, staphylococcal c ... | 1989 | 2781867 |
| [comparative evaluation of the levels of water-soluble glial fibrillary acidic protein in the human brain in the normal state and in schizophrenia]. | the concentration of water-soluble glial fibrillary acidic protein was measured in several regions of human brains (12 schizophrenic patients and 13 patients without any signs of mental disease) using elisa technique. the content of this protein was lower in thalamus and gyrus cinguli in schizophrenics as compared to normals (p less than 0.01). the protein showed no correlation with sex, age and the terms of autopsy (5 to 7 h after death). the findings suggest that decreasing concentration of th ... | 1989 | 2781906 |
| quantitative assessment of the biological effects of follicle regulatory protein on dihydrotestosterone-maintained spermatogenesis in hypophysectomized rat. | follicle regulatory protein (frp) isolated from porcine ovarian follicles influences folliculogenesis through a paracrine mechanism. a similar protein has been found in the testes and seems to have some inhibitory effects on spermatogenesis when administered to intact male experimental animals. on the basis of female and male studies, it has been ascertained that the effects of frp are at the level of gonads and not the pituitary or the hypothalamus. in the studies with intact males it was not p ... | 1989 | 2782630 |
| human eosinophils express cd4 protein and bind human immunodeficiency virus 1 gp120. | the cd4 glycoprotein, expressed on leukocytes belonging to subsets of t lymphocytes and to cells of monocyte/macrophage lineage, participates in the functioning of t cells and serves as a receptor for hiv-1 and hiv-2. human eosinophils, a class of granulocytic leukocytes, have been found to express cd4. with anti-cd4 mabs cd4 was demonstrable on eosinophils from both normal and eosinophilic donors. eosinophils synthesized a 55-kd cd4 polypeptide immunoprecipitable with two anti-cd4 mabs. eosinop ... | 1989 | 2783333 |
| bone marrow progenitor cells induce a regulatory autologous proliferative t lymphocyte response. | the proliferative response of human t lymphocytes to autologous bone marrow progenitor cells was studied by in vitro coculture in autologous serum. irradiated enriched bone marrow progenitor cells induced the proliferation of cocultured peripheral blood t cells, with maximal proliferation at 8 days and stimulator:proliferator ratios of 1/1. this autologous proliferative t lymphocyte response was completely abrogated by the inclusion of anti-hla-dr, anti-cd2, or anti lfa-3 antibodies into the coc ... | 1989 | 2783600 |
| neurochemical profile of moclobemide, a short-acting and reversible inhibitor of monoamine oxidase type a. | moclobemide belongs to a new generation of short-acting, reversible, monoamine oxidase (mao) inhibitors. in vitro (rat brain homogenates) moclobemide inhibits mao-a selectively with lower potency than many of the reference mao inhibitors. however, when measured ex vivo in the rat, the potency of moclobemide is similar to that of reference compounds. in vivo the drug induces a dose-dependent, short-lasting (8-16 hr) and preferential inhibition of mao-a in the brain and both mao-a and mao-b inhibi ... | 1989 | 2783611 |
| neurochemical profile of moclobemide, a short-acting and reversible inhibitor of monoamine oxidase type a. | moclobemide belongs to a new generation of short-acting, reversible, monoamine oxidase (mao) inhibitors. in vitro (rat brain homogenates) moclobemide inhibits mao-a selectively with lower potency than many of the reference mao inhibitors. however, when measured ex vivo in the rat, the potency of moclobemide is similar to that of reference compounds. in vivo the drug induces a dose-dependent, short-lasting (8-16 hr) and preferential inhibition of mao-a in the brain and both mao-a and mao-b inhibi ... | 1989 | 2783611 |
| introduction of a ha-ras oncogene into rat liver epithelial cells and parenchymal hepatocytes confers resistance to the growth inhibitory effects of tgf-beta. | growth of rat liver epithelial cells (rlec) and primary cultures of parenchymal hepatocytes is potently inhibited by tgf-beta. transfection of a mutated ha-ras oncogene, but not a human c-myc oncogene, into rlec resulted in cell lines resistant to growth inhibition by tgf-beta under anchorage-dependent conditions. infection of primary rat hepatocyte cultures with v-ha-ras yielded a cell line likewise insensitive to inhibition by tgf-beta. binding of [125i]tgf-beta to ha-ras-transfected rlec was ... | 1989 | 2783773 |
| characteristics of human synovial fibroblast activation by il-1 beta and tnf alpha. | human synovial fibroblast cell lines (hsn), established from tissues obtained from the knee joints of arthritis patients undergoing arthoplasty, were used to investigate the effects of human interleukin-1 (il-1) beta and tumour necrosis factor (tnf)alpha on proliferation and prostaglandin e2 (pge2) secretion. il-1 beta and tnf alpha were equipotent stimulators of hsn proliferation. classical non-steroidal anti-inflammatory drugs and glucocorticoids significantly augmented this effect. in additio ... | 1989 | 2784408 |
| post-irradiation regeneration of early b-lymphocyte precursor cells in mouse bone marrow. | to examine the sequential development of early b-cell precursors in mouse bone marrow, b-lineage cells have been examined during a wave of post-irradiation regeneration. cell phenotypes have been defined using double-immunofluorescence labelling techniques for (i) terminal deoxynucleotidyl transferase (tdt); (ii) b220 glycoprotein, detected by the binding of mab 14.8; (iii); mu heavy chains in the cytoplasm (c mu) and at the cell surface (s mu). three populations of mu- cells (tdt+14.8-; tdt+14. ... | 1989 | 2784779 |
| tumor necrosis factor-alpha activation of the il-2 receptor-alpha gene involves the induction of kappa b-specific dna binding proteins. | tnf-alpha induces the expression of il-2r and promotes the proliferation and differentiation of t and b cells. in this report, we have studied the biochemical basis for tnf-alpha activation of the il-2r alpha (tac, p55) gene. transfection of human t cell lines with selectively mutated forms of the il-2r alpha promoter revealed that a kappa b element (nucleotides -267 to -256), as well as 5' flanking sequences (nucleotides -281 to -271) are required for tnf-alpha induction of this transcriptional ... | 1989 | 2785134 |
| production of human cells expressing individual transferred hla-a,-b,-c genes using an hla-a,-b,-c null human cell line. | we detail in this report the characterization of a human b-lymphoblastoid cell line, .221, that does not express endogenous hla-a, hla-b, or hla-c class i ag due to gamma-ray-induced mutations in the hla complex. mutant .221 is characterized by: 1) complete absence of hla-a,-b,-c mrna transcripts and alpha-chains, and 2) intracellular expression of two non-a,-b,-c class i alpha-chains with an abundance less than or equal to 1% of normal hla-a,-b,-c expression on similar cells. however, transferr ... | 1989 | 2785140 |
| trophic effects of first-trimester human trophoblasts and human chorionic gonadotropin on lymphocyte proliferation. | we examined the effects of pure first-trimester human trophoblast cells grown in long-term cultures or their secreted products on the proliferation of human peripheral blood lymphocytes cultured alone, under allogeneic stimulation, or in the presence of concanavalin a. both trophoblasts and their culture supernatants stimulated lymphocyte proliferation. culture supernatant had a moderate enhancing effect on lymphocyte mitogenesis in mixed lymphocyte cultures and in the presence of concanavalin a ... | 1989 | 2785344 |
| two cases of pneumocystis carinii pneumonia occurred in human immunodeficiency virus infected patients: supplemental treatment with aerosolised pentamidine isethionate. | two cases of pneumocystis carinii pneumonia occurred in human immunodeficiency virus infected patients who were diagnosed by the aspiration-lung-biopsy. they were treated with 4 mg/kg of pentamidine isethionate intravenously administered followed by pentamidine aerosol inhalation. although clinical and laboratory findings were improved by the fifth or seventh day of intravenous therapy, substantial leukocytopenia, from 3,000/cmm to 600/cmm and from 2,700/cmm to 1,000/cmm, occurred. at this point ... | 1989 | 2786100 |
| co-localization of corticotropin-releasing factor- and enkephalin-like immunoreactivities in nerve cells of the rat hypothalamus and adjacent areas. | the co-localization of corticotropin-releasing factor (crf)- and enkephalin (enk)-like immunoreactivities in nerve cells of the rat hypothalamus and adjacent areas was investigated by the simultaneous application of immuno-beta-galactosidase staining and the peroxidase-antiperoxidase (pap) method to the same sections. crf-like immunoreactive cells were stained blue with immuno-beta-galactosidase staining and enk-like immunoreactive cells brown with the pap method. double-labeled cells with overl ... | 1989 | 2786446 |
| human t lymphocytes possess a limited in vitro life span. | the t lymphocyte offers certain theoretical advantages over other available cell types for the study of aging. immunosenescence is a well-established part of, and may be directly relevant to, mammalian aging, and the t lymphocyte is well-characterized as to function, cell-surface antigen make-up, and other factors. however, prior efforts at studying in vitro aging of t cells have been hampered by poor reproducibility in doubling potential and the occurrence of a peculiar type of crisis. we have ... | 1989 | 2786475 |
| sv40 t antigen acts as a minor histocompatibility antigen of sv40 t antigen tolerant transgenic mice. | the ability of normal mice to mount an sv40 t antigen-specific cytolytic t lymphocytes response when immunized in vivo with splenocytes from the sv40 t antigen transgenic 427-line mice and restimulated in vitro with sv40-transformed fibroblasts, or when immunized with sv40 and restimulated with 427-line splenocytes, was analyzed. both immunization schemes resulted in an sv40 t antigen-specific immune response, indicating the presence of sv40 t antigen-positive cells in the spleens of these trans ... | 1989 | 2786497 |
| enhanced antitumor effect of combination therapy with interleukin-2 and polyribonucleotides against adenocarcinoma 755. | human recombinant interleukin-2 (il-2) and interferon (ifn) inducers in combination were evaluated for their in vivo antitumor efficacy in relation to s.c.-implanted adenocarcinoma 755 in c57bl/6 mice. two ifn inducers, polyinosinic-polycytidylic acid [poly(i)poly(c)] and polyadenylic-polyuridylic acid [poly(a)poly(u)], induced high ifn production in various tissues for a long time compared to treatment with murine interferon-beta. especially, poly(i)poly(c) at 5 mg/kg, the maximum tolerated dos ... | 1989 | 2786554 |
| cholinergic contraction to field stimulation in coronary arteries of cattle. | epicardial coronary artery strips of cattle hearts mounted in vitro respond to transmural stimulation with a neurogenic constriction, attributable to the endogenous release of acetylcholine. these responses were elicited with frequencies as low as 1.0 hz and with pulse durations as low as 100 microseconds. the magnitude of the contractile response increased with increasing frequency of stimulation. blockade of adrenergic neuronal mechanisms with guanethidine (5 x 10(-6) m) increased the size of ... | 1989 | 2786563 |
| systemic delayed-type hypersensitivity to cholera toxin and a detoxified derivative. | the delayed-type hypersensitivity (dth) response to cholera toxin (ct) and an immunopurified formalinized toxoid (td) was studied in adult balb/c mice. intradermal injection of ct in non-immune animals produced substantial footpad oedema which interfered with the measurement of specific dth but td proved satisfactory as a challenge antigen. dth to ct was induced by doses ranging from 0.1 to 10 micrograms, in complete freund's adjuvant (cfa). the optimal reaction was primed for by 1 microgram ct. ... | 1989 | 2786775 |
| abnormalities in growth characteristics of aortic smooth muscle cells in spontaneously hypertensive rats. | comparative studies have shown that cultured vascular smooth muscle cells from spontaneously hypertensive rats (shr) proliferate to a higher cell number, grow to a greater density, and have greater specific growth rate, particularly at a higher saturation density, than those of the normotensive wistar-kyoto (wky) control rats. the growth difference was not due to varying cell survival nor to attachment ability after passage. the degree of dna synthesis was estimated by [3h]thymidine incorporatio ... | 1989 | 2786847 |
| an alternative cytoplasmic domain of the integrin beta 3 subunit. | a cdna encoding a new form of the shared beta subunit (beta 3) of the platelet integrin gpiib/iiia and the vitronectin receptor was isolated from a placental cdna library by screening with a beta 3 (gpiiia) dna probe. this beta 3 variant differs from the previously reported beta 3 in that the cytoplasmic domain is 8 amino acids shorter and has an alternative, 13-amino acid cooh-terminal peptide. the 3' untranslated region of the cdna also differs from the previously reported sequence, while the ... | 1989 | 2787511 |
| effect of protein kinase c inhibitor (h-7) and calmodulin antagonist (w-7) on pertussis toxin-induced il-1 production by human adherent monocytes. comparison with lipopolysaccharide as a stimulator of il-1 production. | human adherent monocytes stimulated with 1 microgram/ml pertussis toxin (pt) produced interleukin-1 (il-1), as measured by thymocyte co-stimulation assay and enzyme-linked immunosorbent assay (elisa), specific for il-1 alpha and il-1 beta. to clarify the role of protein kinase c (pkc) and calmodulin in il-1 production, we investigated the effects of a pkc inhibitor, h-7, and a calmodulin antagonist, w-7 on pt- and lipopolysaccharide (lps)-induced il-1 production by monocytes. addition of 10 micr ... | 1989 | 2787778 |
| differential exposure of the major gangliosides in rabbit thymocytes to vibrio cholerae neuraminidase. | neuraminidase treatment of lymphocytes is known to cause changes of cellular responses in several biological phenomena, but the molecules modified on the cell surface by neuraminidase are not known in detail. rabbit thymocytes, which contain tissue-characteristic gangliosides, were treated with vibrio cholerae neuraminidase, and the susceptibility of the cell surface sialic acid residues was examined. the amount of sialic acid released from the thymocytes at the highest level was 42.4 nmol per 1 ... | 1989 | 2787793 |
| inhibition of enteropathogenic bacteria by human milk whey in vitro. | diarrhea caused by enteropathogenic bacteria is a leading cause of childhood mortality world-wide, particularly in less developed regions. breast-feeding has been advocated to protect infants and children from infectious illnesses. we examined the antibacterial activity of human whey in vitro against multiple strains of the following species of enteropathogenic bacteria, campylobacter jejuni, escherichia coli, salmonella typhimurium, shigella flexneri, shigella sonnei and vibrio cholerae, all is ... | 1989 | 2787902 |
| effect of doxorubicin on mouse hybridoma b cells: stimulation of immunoglobulin synthesis and secretion. | the purpose of these studies was to investigate whether the cell-differentiating effect of anthracyclines can trigger an over-production and secretion of molecules that may interfere with the tumor-host relationship. we exposed mouse hybridoma b-cells, which are devoted to immunoglobulin production, to doxorubicin (10-40 ng/ml). we found that most doxorubicin-treated cells secreted 3- to 5-fold higher amounts of immunoglobulin than untreated cells, along with an accumulation of 50% of them in th ... | 1989 | 2788496 |
| a t-cell-specific transcriptional enhancer element 3' of c alpha in the human t-cell receptor alpha locus. | a transcriptional enhancer element has been identified 4.5 kilobases 3' of c alpha (constant region alpha chain) in the human t-cell receptor (tcr) alpha-chain locus. this enhancer is active on both a tcr v alpha (variable region alpha chain) promoter and the minimal simian virus 40 promotor in tcr alpha/beta jurkat and el4 cells but is inactive on a v alpha promoter in human tcr gamma/delta peer and molt-13 cells, clone 13 b cells, and hela fibroblasts. the enhancer has been localized to a 116- ... | 1989 | 2788889 |
| effect of vitamin d metabolites on calcitriol metabolism in experimental renal failure. | previous studies from our laboratory have demonstrated that metabolic clearance rate (mcr) of calcitriol is decreased in experimental renal failure. in this experiment, we examined the effects of calcitriol, 25-hydroxyvitamin d3 (25(oh)d3) and 24,25-dihydroxyvitamin d3 (24,25(oh)2d3) on the mcr of calcitriol in renal failure produced in rats by partial nephrectomy. the mcr of calcitriol in these rats with renal failure was significantly lower than in control rats with sham operations. plasma con ... | 1989 | 2789310 |
| potentiation by vestibular stimulation of the immobility reflex elicited by clamping in developing and adult rats. | we have previously described (exp. neurol., 97: 315-326, 1987) that clamping of the neck elicits a profound immobility with flexion of limbs and ventroflexion of head and neck ('carrying posture'). we also observed that when a clamped rat pup is carried by the experimenter's hand, such carrying posture was potentiated. in the present paper we observed that in adult rats vestibular stimulation by circular acceleration potentiates the duration of immobility reflex by clamping and intensifies the l ... | 1989 | 2789702 |
| squamous cell carcinoma of the base of the tongue: results of treatment in 115 cases. | between 1976 and 1986, we treated 115 patients (mean age 53.8 years) with base of tongue carcinomas. the staging system used was the uicc tnm classification of 1979. seventy per cent of the tumours were t3 or t4 and 42% had n2 or n3 lymph node. locoregional treatment was irradiation alone (98/115) or surgery and post-operative radiotherapy (17/115). sixty-seven patients received induction chemotherapy. actuarial survival of the entire group at 3 and 5 years was 25 and 23%, respectively, and 3-ye ... | 1989 | 2790426 |
| [clinical and experimental research on a kidney-tonifying prescription in preventing and treating children's hearing-loss induced by aminoglycoside antibiotic ototoxicity]. | this paper reports 30 cases of hearing-loss induced by aminoglycoside antibiotic ototoxicity treated mainly with kidney-tonifying herbs such as herba epimedii, rhizoma drynariae, rhizoma polygonati, radix polygoni multiflori, magnetitum and rhizoma acori graminei, etc. after three month's treatment, the authors found that 19 cases had improved their hearing more than 15 db, among which there are 8 cases who had improved 15 db and 11 cases 30 db. the patients' hearing of pre-treatment and post-tr ... | 1989 | 2791158 |
| relative contribution of various forms of cytochrome p450 to the metabolism of benzo[a]pyrene by human liver microsomes. | a variety of cytochromes p450 have been implicated in the hepatic metabolism of benzo[a]pyrene (bp), including forms that are constitutively expressed and those that are highly inducible. in the present study the metabolism of bp to organic solvent-soluble derivatives by eight forms of cytochrome p450 isolated from rat liver and by a series of 11 human liver microsomal samples was investigated. the relative contribution of specific p450 forms to the human hepatic metabolism was evaluated. a 4-fo ... | 1989 | 2791199 |
| effect of platelet factors on migration of cultured bovine aortic endothelial and smooth muscle cells. | endothelial cell (ec) injury and the response of ec and smooth muscle cells (smcs) to injury contribute to the pathophysiology in patients with vascular disease and atherosclerosis. since platelets have been suggested to play an important role in modulating vascular injury, the present study was undertaken to examine the influence and mechanism of action of individual platelet factors on bovine aortic ec and smc migration using an in vitro wound assay system. serotonin decreased ec proliferation ... | 1989 | 2791219 |
| intensive care for patients with pneumocystis carinii pneumonia and respiratory failure. are we prepared for our new success? | | 1989 | 2791661 |
| [ferric ammonium citrate as virulence-enhancing agent in the toxicity test of vibrio cholerae and the potency assay of cholera vaccine]. | using icr strain mice as experimental animals, 0.25% (w/v) ferric ammonium citrate (fac) was a suitable substitute for 5% (w/v) mucin as a virulence-enhancing (ve) agent in the toxicity test of vibrio cholerae and the potency assay of cholera vaccine. there was no significant lethal toxicity difference between 0.25% fac and 5% mucin as ve agents on vibrio cholerae in mice. by using 0.25% fac as a ve agent in estimating the potency of cholera vaccine, the relative potency (r.p.) of the tested cho ... | 1989 | 2791724 |
| visual evoked potentials in prader-willi syndrome. | oculocutaneous, electrophysiological, and cytogenetic factors were evaluated in 14 patients with prader-willi syndrome and in three controls, two albinos and a normal observer. in a substantial number of pw patients chromosomal anomalies, particularly deletions of the long arm of chromosome 15 as well as hypopigmentation of hair, skin, and eye have been identified. in the genetic condition of albinism, hypopigmentation related to neural ectoderm derivatives is associated with reduced visual acui ... | 1989 | 2791841 |
| interaction between prostatic fibroblast and epithelial cells in culture: role of androgen. | we have established and characterized two cell lines from the ventral prostate gland of normal nb rats: nbe-1 (prostatic epithelial) and nbf-1 (prostatic fibroblast) cell lines. to identify the direct mitogenic action of dihydrotestosterone (dht), we incubated these cell lines alone and together (in the presence and absence of cell contact) with various concentrations of dht (0.1-10,000 ng/ml) for 24-72 h and assayed for the rate of dna synthesis and the total number of cells in tissue culture a ... | 1989 | 2792005 |
| bolus injections of recombinant human interleukin-1 cause transient hypocalcemia in normal mice. | single daily injections of recombinant human interleukin-1 alpha (il-1 alpha) or interleukin-1 beta (il-1 beta) were given for 1-3 days to normal mice, and blood ionized calcium concentrations were measured before and at various times after each injection. mean blood calcium levels fell significantly in both groups of animals 3 h after the first il-1 injections and returned to pretreatment values at 5 h. however, at 24 and 48 h mean values were significantly higher than those in saline-heated co ... | 1989 | 2792008 |
| mechanisms of arterial thrombosis in nonparallel streamlines: platelet thrombi grow on the apex of stenotic severely injured vessel wall. experimental study in the pig model. | the role of thrombosis in various acute coronary syndromes has been established. however, the basic mechanism by which plaque rupture leads to a growing thrombus in the vicinity of stenotic lesions is not well understood. using a characterized flow chamber in a rheologically controlled system, we have mimicked stenotic vessels and studied for the first time cell-vessel wall interaction in nonparallel streamlines. stenoses ranging from 0 to 80% were produced with stripped tunica media to mimic se ... | 1989 | 2794050 |
| a case of non-o:1 vibrio cholerae bacteremia from northern europe. | | 1989 | 2794570 |
| mutation induced by vibriophage ps166 infection changes biotype and phage type of vibrio cholerae. | the effect of a newly isolated vibriophage, ps166, on vibrio cholerae (e1 tor) mak757 was investigated. two ps166-resistant mutants of strain mak757 were isolated. these had undergone transition to the classical biotype with concomitant acquisition of new phage sensitivity. however, the parental ogawa serotype remained unchanged. these mutant strains also showed a unique temperate phage-sensitivity profile, distinct from that of strain mak757. the possible target of phage ps166 interaction is di ... | 1989 | 2795637 |
| [monoclonal antibodies to antigens of cholera vibrios of the ogava serovar]. | a set of hybrids is obtained synthesizing monoclonal antibodies to the surface antigenic determinants of the choleric vibrio of the ogava serovar. the antigenic structure of vibrios of the typical and atypical strains isolated from a man and from environmental objects is studied using a collection of highly specific immunoglobulins. the high-specific diagnostic preparations for identification of the 01-group cholera agent serovar may be created on their base. | 1989 | 2796789 |
| the activity of phosphoenolpyruvate carboxykinase throughout the lactation cycle of the guinea pig mammary gland. | the enzyme phosphoenolpyruvate carboxykinase (pepck) has been measured in the guinea pig mammary gland throughout the pregnancy-lactation cycle. this is of interest since the primary importance of pepck is thought to be its role in gluconeogenesis and it is questionable whether or not gluconeogenesis occurs in the mammary gland. the enzyme activity, present in both the cytosol and mitochondria, was shown to follow the lactation profile. during the transition into lactation, cytosolic pepck activ ... | 1989 | 2798434 |
| the activity of phosphoenolpyruvate carboxykinase throughout the lactation cycle of the guinea pig mammary gland. | the enzyme phosphoenolpyruvate carboxykinase (pepck) has been measured in the guinea pig mammary gland throughout the pregnancy-lactation cycle. this is of interest since the primary importance of pepck is thought to be its role in gluconeogenesis and it is questionable whether or not gluconeogenesis occurs in the mammary gland. the enzyme activity, present in both the cytosol and mitochondria, was shown to follow the lactation profile. during the transition into lactation, cytosolic pepck activ ... | 1989 | 2798434 |
| [tuberculosis of the lymph nodes and spleen preceding hodgkin's disease]. | tuberculosis of the lymph nodes is not rare today. however tuberculosis of the spleen has been very rare even in the past when many patients had suffered from tuberculosis. the appearance of hodgkin's disease following tuberculosis is an extremely rare condition. it has thought that deficiency of cellular immunity was responsible for both tuberculosis and malignancy. we present a 44-year-old man in whom lymph node and spleen tuberculosis antedated the clinical onset of hodgkin's disease for thre ... | 1989 | 2799551 |
| survival of vibrio cholerae in african domestic water storage containers. | although much has been published about growth of vibrio cholerae in natural sources of water, little or nothing has been written on its survival in common household water storage containers. this study compared the behaviour of the el tor and classical biotypes of v. cholerae in different containers commonly used in africa for domestic water storage. these included traditional clay pots, wooden barrels, galvanised iron drums (corroded and non-corroded) and plastic (polyethylene) drums. the findi ... | 1989 | 2799584 |
| hemagglutination and intestinal adherence properties of clinical and environmental isolates of non-o1 vibrio cholerae. | hemagglutination and intestinal adherence properties of non-o1 vibrio cholerae were studied in vitro. no definite correlation between the cell-associated hemagglutinin titers and the intestinal adhesion indices was noted. sugar- and glycoprotein-mediated inhibition data also indicated differences between the hemagglutination and adherence processes in respect to the receptor structures. intestinal adherence of most v. cholerae strains could be inhibited to various extents by n-acetyl d-glucosami ... | 1989 | 2802613 |
| a case report of acute obstructive suppurative cholangitis in a non-0-1 vibrio cholerae biliary carrier. | a case report is presented of a 73-year-old male who was seen with fever, jaundice, abdominal pain and central nervous system depression. he failed to respond to intensive antibiotic therapy, and subsequently acute obstructive suppurative cholangitis fully developed. upon laparotomy, the patient's gallbladder was found to be enlarged with the bile from the gallbladder and bile duct itself containing a high pus content. its cultured organism revealed non-0-1 vibrio cholerae. to our knowledge, no ... | 1989 | 2802912 |
| [studies on tissue distribution of unusual estrogen-binding protein in rats with the help of immunoblotting]. | tissue distribution in male and female rats of unusual immunoreactive material specific for the hepatic estrogen-binding protein (uebp) was studied by means of immunoblotting analysis. from 28 organs and tissues studied only the liver of animals of both sexes was found to contain polypeptide with mr, close to mr of purified uebp (approximately 31,000), which reacts with anti-uebp specific antiserum. detection of the immunoreactive material in female liver, which does not contain any hormone-bind ... | 1989 | 2804329 |
| [studies on tissue distribution of unusual estrogen-binding protein in rats with the help of immunoblotting]. | tissue distribution in male and female rats of unusual immunoreactive material specific for the hepatic estrogen-binding protein (uebp) was studied by means of immunoblotting analysis. from 28 organs and tissues studied only the liver of animals of both sexes was found to contain polypeptide with mr, close to mr of purified uebp (approximately 31,000), which reacts with anti-uebp specific antiserum. detection of the immunoreactive material in female liver, which does not contain any hormone-bind ... | 1989 | 2804329 |
| detection of factor x activation in humans. | a sensitive radioimmunoassay (ria) for the fragment that is liberated from factor x when this zymogen is activated by factor vii/viia-tissue factor or factor ixa was developed. antisera were raised in rabbits to a synthetic 15 amino acid peptide containing the cooh-terminal sequence of the activation fragment coupled to bovine serum albumin with glutaraldehyde. the reactivity of the antibody population obtained toward the factor x zymogen was negligible (less than 1/36,000 that of the activation ... | 1989 | 2804345 |
| detection of factor x activation in humans. | a sensitive radioimmunoassay (ria) for the fragment that is liberated from factor x when this zymogen is activated by factor vii/viia-tissue factor or factor ixa was developed. antisera were raised in rabbits to a synthetic 15 amino acid peptide containing the cooh-terminal sequence of the activation fragment coupled to bovine serum albumin with glutaraldehyde. the reactivity of the antibody population obtained toward the factor x zymogen was negligible (less than 1/36,000 that of the activation ... | 1989 | 2804345 |
| properties of cutaneous afferents in diabetic neuropathy. | the technique of percutaneous microneurography was used to study the response properties of 68 cutaneous afferents in 8 diabetic patients and, for comparison, of 104 afferents in 12 normal control subjects. the diabetic patients were neurologically asymptomatic or had a mild sensory neuropathy. three afferent categories were studied in the diabetic subjects (rapidly adapting type ra and slowly adapting sai and saii) in response to mechanical skin stimulation and abnormal responsiveness was encou ... | 1989 | 2804617 |
| formation and removal of dna adducts in target and nontarget tissues of rats administered multiple doses of 2-acetylaminophenanthrene. | 2-acetylaminophenanthrene (2-aap) is carcinogenic for the mammary gland, small intestine and ear duct in rats, but is not hepatocarcinogenic. in order to understand the tissue specificity of tumor induction, the formation and removal of dna adducts in target and nontarget tissues have been compared in rats administered 2-aap. male and female sprague--dawley rats were treated i.p. with up to four weekly doses of 5 mg 2-aap per kg body weight. 32p-post-labeling analysis of the dna from all tissues ... | 1989 | 2805226 |
| formation and removal of dna adducts in target and nontarget tissues of rats administered multiple doses of 2-acetylaminophenanthrene. | 2-acetylaminophenanthrene (2-aap) is carcinogenic for the mammary gland, small intestine and ear duct in rats, but is not hepatocarcinogenic. in order to understand the tissue specificity of tumor induction, the formation and removal of dna adducts in target and nontarget tissues have been compared in rats administered 2-aap. male and female sprague--dawley rats were treated i.p. with up to four weekly doses of 5 mg 2-aap per kg body weight. 32p-post-labeling analysis of the dna from all tissues ... | 1989 | 2805226 |
| fatty-acid-binding protein from bovine brain. amino acid sequence and some properties. | a fatty-acid-binding protein (fabp) from the cytosol of bovine brain was purified by sephadex g-75 filtration and electrofocusing. the purified fabp behaved as an anionic protein with an apparent molecular mass of 14.7 kda; its complete amino acid sequence was determined and microheterogeneity was observed. sequence comparison with other fabps of known sequence and the observed microheterogeneity demonstrated the presence in brain of several homologous fabps closely related to heart fabp and bov ... | 1989 | 2806261 |
| pancreaticoduodenal graft in the rat: an original microsurgical technique. | the authors describe an original surgical technique for pancreatic transplantation carried out on an experimental rat model which duplicates as closely as possible the anatomic-functional situation of a pancreatic graft in man (one arterovenous vascular peduncle with one functioning kidney only). the technique entails a microanastomosis between the mesenteric artery and the portal vein of the donor organ with the left renal artery and vein of the recipient. this method appears to be technically ... | 1989 | 2806343 |
| [the effect of progesterone, testosterone and estradiol on steroid hormone release by the ovarian follicles of cows in vitro]. | | 1989 | 2806660 |
| [study of polymorphism of strains of vibrio cholerae of various origins using a method of genomic fingerprinting]. | population of atoxigenic eltor vibrios (vct-) isolated from open water has been shown to be heterogeneous by the genomic fingerprinting technique. epidemically dangerous strains (vct+) containing the gene coding for cholera toxin have similar hybridization profile independent of time and place of their isolation. the clonal formation of vct+ strains and the absence of vct----vct+ transition of strains under natural conditions are discussed. | 1989 | 2806903 |
| molecular characterization of trp1, a gene coding for tryptophan synthetase in the basidiomycete coprinus cinereus. | we utilized a cloned gene (trp5) encoding tryptophan synthetase (tsase) from saccharomyces cerevisiae to identify and clone the corresponding gene (trp1) from the basidiomycete coprinus cinereus. the primary nucleotide (nt) sequence of this gene was determined and compared to sequences from other filamentous fungi, as well as to other genes coding for tsase. a transformation assay was used to demonstrate that 321 nt, which do not include caat or tataaa elements and precede the translation initia ... | 1989 | 2806911 |
| preliminary assessment of the safety and immunogenicity of live oral cholera vaccine strain cvd 103-hgr in healthy thai adults. | a single dose (5 x 10(8) organisms) of attenuated a- b+ vibrio cholerae classical inaba recombinant vaccine strain cvd 103-hgr or placebo was administered to 24 healthy young thai adults in a randomized, placebo-controlled, double-blind trial of safety and immunogenicity. none of the volunteers experienced untoward reactions. the vaccine strain was recovered from 2 of 12 vaccines. the vibriocidal antibody response (the best immunological correlate of protection) was good: 11 of 12 vaccinees (92% ... | 1989 | 2807523 |
| development of location-specific hair cell stereocilia in denervated embryonic ears. | the developmental mechanisms that allow physiological coding of acoustic pitch have remained unexplained. cochlear hair cells that have different structures respond to different sound frequencies and synapse with neurons that project to different locations in the brain. how do these hair cells develop appropriate structures, and how are the connections between specific hair cells and the neurons that code for their pitch sensitivities matched? we have investigated one aspect of this by denervati ... | 1989 | 2808748 |
| states of the art of immunoelectron microscopy in japan. | in japan, there are several laboratories which routinely utilize immunoelectron microscopy (iem) for their investigation and participate in the development of methods for iem. as a result, some major advances are in the making. iem methods used in japan may be divided roughly into four categories: 1) antibodies are reacted with tissues before the tissues are embedded in plastics (pre-embedding method); 2) antibodies are reacted with tissues after the tissues are embedded in plastics (post-embedd ... | 1989 | 2809470 |
| regulation of insulin-like growth factor-i and growth hormone receptor gene expression by diabetes and nutritional state in rat tissues. | insulin-like growth factor-i (igf-i) mrna and gh receptor mrna levels were analysed in different tissues from rats made diabetic with streptozotocin, fasted rats and rats fed with a protein-reduced diet. diabetes decreased igf-i mrna levels in liver, heart, diaphragm, kidney and aorta, but not in brain. gh receptor mrna levels were decreased in heart and diaphragm, but not in liver and kidney. fasting decreased igf-i mrna in all tissues studied except brain, and decreased gh receptor mrna in liv ... | 1989 | 2809476 |
| an antitumor principle from suillus granulatus. | an antitumor principle from suillus granulatus has been identified as suillin. in vitro and in vivo activities against p-388 leukemia have been determined. | 1989 | 2809609 |
| thyroid follicular carcinoma with pulmonary metastases in a beaver (castor canadensis). | an 11-yr-old female beaver (castor canadensis) died after a 3 1/2 mo course of intermittent diarrhea, lethargy and anorexia. a postmortem examination revealed both a necrotizing ulcerative colitis and bilaterally enlarged thyroid glands. histologically, the necrotizing colitis was similar to that caused by canine or feline parvovirus. thyroid glands were multilobulated. lobules were composed of irregularly arranged, variably sized follicles, some of which contained colloid. follicles were lined ... | 1989 | 2810561 |
| [clinical laboratory approach for estimating effective administrative dose of cephalothin. reevaluation of mic break points in 4 category system of disc susceptibility test]. | the reliability of the cephalothin (cet) disc susceptibility test in estimating approximate values of mics was studied using various clinical isolates totaling 248 strains and using showa discs (8 mm diameter containing 30 micrograms of cet) and difco discs (6 mm diameter containing 30 micrograms of cet). clinical significance of a 4 category system for the interpretation of the cet disc tests, which is widely used in japan, was reevaluated to determine whether this system would be suitable or n ... | 1989 | 2810753 |
| cathepsin b in cells of two rat sarcomas with different rates of spontaneous metastasis. | the cysteine proteinase cathepsin b (ec 3.4.22.1) is believed to take part in biochemical processes underlying tumor metastasis. in the present study, the cellular localization, intracellular levels and extracellular release of cathepsin b activity were examined in vitro in cells of two rat sarcoma variants, lw13k2 and rps, differing in their capacity to metastasize spontaneously to the lung of syngeneic lew/cub rats. the lw13k2 sarcoma metastasizes rarely, whereas the lw13k2-derived rps variant ... | 1989 | 2812149 |
| cathepsin b in cells of two rat sarcomas with different rates of spontaneous metastasis. | the cysteine proteinase cathepsin b (ec 3.4.22.1) is believed to take part in biochemical processes underlying tumor metastasis. in the present study, the cellular localization, intracellular levels and extracellular release of cathepsin b activity were examined in vitro in cells of two rat sarcoma variants, lw13k2 and rps, differing in their capacity to metastasize spontaneously to the lung of syngeneic lew/cub rats. the lw13k2 sarcoma metastasizes rarely, whereas the lw13k2-derived rps variant ... | 1989 | 2812149 |
| role of carboxylesterase in soman, sarin and tabun poisoning in rats. | tocp (triorthocresyl phosphate) inhibits carboxylesterase (carbe) activity in rat plasma and liver and significantly increases soman, sarin and tabun toxicity. application of these agents after tocp caused strong additional inhibition of carbe and cholinesterases (che) in rat red blood cells, plasma, liver, brain, diaphragm and intercostal muscle. after phenobarbital pretreatment, which induced carbe activity in plasma and liver by 80% and that of plasma che by 33%, acute toxicity of soman and t ... | 1989 | 2813290 |
| etiology of acute childhood diarrhoea in calcutta. | of the 152 cases of acute diarrhoea, 124 (81.5%) revealed potential pathogens. altogether 27 (21.2%) out of 127 strains of escherichia coli, klebsiella pneumoniae, enterobacter, proteus and acinetobacter produced enterotoxin. single pathogenic bacteria (40 cases 26.3%), parasite (6; 6%), rota virus (6; 6%), toxigenic bacteria (19; 12.5%) and mixed agents (37; 24.24.3%) were recorded in 108 cases (71.0%). another 14 (9.2%) cases exclusively revealed moderate to heavy growth of suspected enteric p ... | 1989 | 2815324 |
| differential inhibition of downstream gene expression by the cauliflower mosaic virus 35s rna leader. | the effect of the 600 nucleotide-long camv 35s rna 5' leader sequence on the expression of downstream genes was analyzed both in plant protoplasts and in vitro. for transient expression studies in protoplasts derived from host and nonhost plants, the bacterial chloramphenicol acetyl transferase (cat) gene was fused to the initiation codon of orf vii. the leader sequence reduced cat expression two- to four-fold in protoplasts derived from three host species, but 10- to 50-fold in protoplasts deri ... | 1989 | 2815595 |
| differential inhibition of downstream gene expression by the cauliflower mosaic virus 35s rna leader. | the effect of the 600 nucleotide-long camv 35s rna 5' leader sequence on the expression of downstream genes was analyzed both in plant protoplasts and in vitro. for transient expression studies in protoplasts derived from host and nonhost plants, the bacterial chloramphenicol acetyl transferase (cat) gene was fused to the initiation codon of orf vii. the leader sequence reduced cat expression two- to four-fold in protoplasts derived from three host species, but 10- to 50-fold in protoplasts deri ... | 1989 | 2815595 |
| [establishment and primary application of non-oi vibrio cholerae serotyping system (vbo)]. | a serotyping system for vibrio cholerae non-oi was reported in this paper. with this typing system, 549 strains isolated from guangdong province, fujian province and henan province were typed. the 55 serotypes were found in these strains. among this, the dominant types were vbo 2, 7 and 9. the distribution of serotypes related with the different region. in our experiment, 19 serotypes were new types, different from sakazaki or smith strains. 83.79% tested strains can be typed with this vbo serot ... | 1989 | 2815719 |
| the dermatopathologist and human immunodeficiency virus infection. | | 1989 | 2817921 |
| [visualization of forensically important foreign bodies by direct roentgen magnification]. | the detection of foreign bodies of microscopic dimensions with radiographic techniques is limited by the size and density of the particle and the radiographic resolution. these restrictions can be of decisive value in case work. the high grade direct radiographic magnification technique was successfully used in preceding experiments. due to its 1 mu focal spot, this tube allows direct magnifications up to 250 times. in present studies, the advantages as well as the limitations of this new techni ... | 1989 | 2818477 |
| [visualization of forensically important foreign bodies by direct roentgen magnification]. | the detection of foreign bodies of microscopic dimensions with radiographic techniques is limited by the size and density of the particle and the radiographic resolution. these restrictions can be of decisive value in case work. the high grade direct radiographic magnification technique was successfully used in preceding experiments. due to its 1 mu focal spot, this tube allows direct magnifications up to 250 times. in present studies, the advantages as well as the limitations of this new techni ... | 1989 | 2818477 |
| production and partial characterization of elastase of vibrio cholerae inaba. | conditions are described for the production of extracellular elastase by vibrio cholerae inaba 2/57. the yield of the enzyme was maximum in shake cultures grown in alkaline peptone water at 37 degrees c and was stable in culture supernatants. the enzyme, partially purified by ammonium sulphate precipitation and sephadex g-100 gel filtration showed a molecular weight of 30,000 and an activity between ph 5.0-8.0 with an optimum of 8.0 in tris-maleate buffer. the elastinolytic activity was maximal ... | 1989 | 2818795 |
| comparison of the structural characteristics of cell-cam 105 from hepatocytes with those of an altered form expressed by rat transplantable hepatocellular carcinomas. | the structural features of the adult rat hepatocyte (arh) forms of cell-cam 105, a mr 105,000 cell adhesion molecule, were compared using a variety of immunochemical and biochemical techniques with altered forms of more basic pi present on two transplantable hepatocellular carcinomas (thc 1682c and thc as-30d). immunoprecipitation analysis with polyclonal (anti-gp 105-2) and monoclonal (mab) antibodies specific for cell-cam 105 (mab 362.50) demonstrated that arh and thc cell-cam 105 were indisti ... | 1989 | 2819719 |
| regulation of nicotinic acetylcholine receptor phosphorylation in rat myotubes by forskolin and camp. | the nicotinic acetylcholine receptor (acchor) from rat myotubes prelabeled in culture with [32p]orthophosphate was isolated by acetylcholine affinity chromatography followed by immunoaffinity chromatography. under basal conditions, the nicotinic acchor was shown to be phosphorylated in situ on the beta and delta subunits. regulation of acchor phosphorylation by camp-dependent protein kinase was explored by the addition of forskolin or camp analogues to prelabeled cell cultures. forskolin, an act ... | 1987 | 2819883 |
| tn602: a naturally occurring relative of tn903 with direct repeats. | we report the characterization of tn602, a transposon encoding resistance to kanamycin and related aminoglycosides present on the r-plasmid pgd10. tn602 is highly homologous to the previously characterized tn903, present on the r-plasmid r6, in that it consists of a gene for aminoglycoside-phosphotransferase-3'-i (homologous to that of tn903) flanked by copies of an is-element homologous to is903. tn602 differs from tn903 in the following respects: the flanking is-elements (is602) are in direct ... | 1987 | 2819910 |
| iga multiple myeloma coexistent with atypical adult t-cell leukemia. | we report a 65-yr-old male with adult t-cell leukemia (atl) who developed multiple myeloma (mm) concomitantly. skin lesions and peripheral leukocytosis were noted during the 5-yr observation period. there was abnormal lymphocytosis with indented or lobulated nuclei in the peripheral blood and in the bone marrow. the neoplastic cells reacted with monoclonal antibodies, okt3, okt4, okia1 and anti-tac. his serum was positive for the antibodies to atl-associated antigens. human t-cell leukemia virus ... | 1987 | 2820787 |
| iga multiple myeloma coexistent with atypical adult t-cell leukemia. | we report a 65-yr-old male with adult t-cell leukemia (atl) who developed multiple myeloma (mm) concomitantly. skin lesions and peripheral leukocytosis were noted during the 5-yr observation period. there was abnormal lymphocytosis with indented or lobulated nuclei in the peripheral blood and in the bone marrow. the neoplastic cells reacted with monoclonal antibodies, okt3, okt4, okia1 and anti-tac. his serum was positive for the antibodies to atl-associated antigens. human t-cell leukemia virus ... | 1987 | 2820787 |
| the molybdenum-pterin binding protein is encoded by a multigene family in clostridium pasteurianum. | there are three variants of the molybdenum-pterin binding protein (mop) encoded by three distinct genes in clostridium pasteurianum. nucleotide sequence analysis shows that the three mop genes have greater than 90% homology at the nucleotide level. upstream from the coding region of each mop gene are potential promoter consensus sequences. analysis of mop purified from cells grown under nitrogen-fixing conditions indicates all three genes are expressed. sequence analysis of the three mop genes a ... | 1987 | 2820842 |
| antibodies to polymerized human serum albumin in acute and chronic liver disease. | since antibodies to polyalbumin have been noted to occur in patients with hepatitis or cirrhosis, we investigated sera from 219 patients with a variety of acute and chronic liver diseases with and without hbsag using an elisa. the prevalence of circulating polyalbumin antibodies was shown to be significantly increased over that of healthy controls (6.7%) in patients with autoimmune chronic active hepatitis (67%), acute viral hepatitis (41%) and fulminant hepatic necrosis (38%), but such antibodi ... | 1987 | 2820857 |
| antibodies to polymerized human serum albumin in acute and chronic liver disease. | since antibodies to polyalbumin have been noted to occur in patients with hepatitis or cirrhosis, we investigated sera from 219 patients with a variety of acute and chronic liver diseases with and without hbsag using an elisa. the prevalence of circulating polyalbumin antibodies was shown to be significantly increased over that of healthy controls (6.7%) in patients with autoimmune chronic active hepatitis (67%), acute viral hepatitis (41%) and fulminant hepatic necrosis (38%), but such antibodi ... | 1987 | 2820857 |
| overproduction and purification of the tn3 transposase. | the tn3 transposase accumulated to about 4% of total cell protein in a minicell-producing escherichia coli strain harboring a transposase overproducer plasmid. this accumulation of the transposase seems to be due to four factors: derepression of transcription resulting from inactivation of the repressor gene (tnpr); efficient translation caused by a mutation within the shine-dalgarno (sd) sequence, the dosage effect of the increased plasmid copy number resulting from deletion of the rom gene of ... | 1987 | 2820953 |
| overproduction and purification of the tn3 transposase. | the tn3 transposase accumulated to about 4% of total cell protein in a minicell-producing escherichia coli strain harboring a transposase overproducer plasmid. this accumulation of the transposase seems to be due to four factors: derepression of transcription resulting from inactivation of the repressor gene (tnpr); efficient translation caused by a mutation within the shine-dalgarno (sd) sequence, the dosage effect of the increased plasmid copy number resulting from deletion of the rom gene of ... | 1987 | 2820953 |
| a new sv40-based vector developed for cdna expression in animal cells. | a useful vector, page103, has been developed for the expression of cdna in animal cells using the simian virus 40 (sv40) expression signals. cdna could be expressed easily by inserting it into the multiple cloning sites (hindiii, sali/acci, xbai, bamhi, smai/xmai, kpni/asp718, saci and ecori) of the vector, which are located between the sv40 early promoter and the sv40 early rna processing signals for splicing and polyadenylation. in addition to the above transcription unit, page103 contains the ... | 1987 | 2820955 |
| a new sv40-based vector developed for cdna expression in animal cells. | a useful vector, page103, has been developed for the expression of cdna in animal cells using the simian virus 40 (sv40) expression signals. cdna could be expressed easily by inserting it into the multiple cloning sites (hindiii, sali/acci, xbai, bamhi, smai/xmai, kpni/asp718, saci and ecori) of the vector, which are located between the sv40 early promoter and the sv40 early rna processing signals for splicing and polyadenylation. in addition to the above transcription unit, page103 contains the ... | 1987 | 2820955 |
| isotopic hybrids of nitrogenase. mössbauer study of mofe protein with selective 57fe enrichment of the p-cluster. | previous mössbauer and epr studies of the mofe protein (approximately 30 fe and 2 mo) of nitrogenase have revealed the presence of two unique clusters, namely, the p-clusters (presumably of the fe4s4 type) and the molybdenum- and iron-containing cofactors (or m-clusters). mössbauer components d (approximately 10-12 fe) and fe2+ (approximately 4 fe) represent subsites of the p-clusters while component s (approximately 2 fe) appeared to belong to a separate, unidentified cluster. in order to refin ... | 1987 | 2820958 |
| isotopic hybrids of nitrogenase. mössbauer study of mofe protein with selective 57fe enrichment of the p-cluster. | previous mössbauer and epr studies of the mofe protein (approximately 30 fe and 2 mo) of nitrogenase have revealed the presence of two unique clusters, namely, the p-clusters (presumably of the fe4s4 type) and the molybdenum- and iron-containing cofactors (or m-clusters). mössbauer components d (approximately 10-12 fe) and fe2+ (approximately 4 fe) represent subsites of the p-clusters while component s (approximately 2 fe) appeared to belong to a separate, unidentified cluster. in order to refin ... | 1987 | 2820958 |
| renal dipeptidase is one of the membrane proteins released by phosphatidylinositol-specific phospholipase c. | renal dipeptidase (dehydropeptidase-i, ec 3.4.13.11) was released from pig kidney membrane preparations by treatment with phosphatidylinositol-specific phospholipase c from staphylococcus aureus and bacillus thuringiensis and a phospholipase c preparation from bacillus cereus to a similar extent as alkaline phosphatase. endopeptidase-24.11 and aminopeptidase n were not released by this treatment. after treatment of the membrane fraction with the s. aureus phospholipase c the dipeptidase was conv ... | 1987 | 2822007 |
| renal dipeptidase is one of the membrane proteins released by phosphatidylinositol-specific phospholipase c. | renal dipeptidase (dehydropeptidase-i, ec 3.4.13.11) was released from pig kidney membrane preparations by treatment with phosphatidylinositol-specific phospholipase c from staphylococcus aureus and bacillus thuringiensis and a phospholipase c preparation from bacillus cereus to a similar extent as alkaline phosphatase. endopeptidase-24.11 and aminopeptidase n were not released by this treatment. after treatment of the membrane fraction with the s. aureus phospholipase c the dipeptidase was conv ... | 1987 | 2822007 |
| cloning and expression of the salmonella enterotoxin gene. | this report examines the genetic basis for salmonella typhimurium q1 enterotoxin production. a 918-base-pair xbai-hincii fragment of plasmid pjm17, composed of cholera toxin (ct) coding sequences (ctxab), was used as a gene probe. with this probe, the s. typhimurium enterotoxin was identified on a 6.3-kilobase ecori-psti fragment of chromosomal dna from plasmidless strain q1. we cloned this 6.3-kilobase fragment into escherichia coli rr1. the genetic map of the cloned salmonella enterotoxin (stx ... | 1987 | 2822664 |
| dependence of an adenosine-activated potassium current on a gtp-binding protein in mammalian central neurons. | neurons in hippocampal and striatal cell cultures respond to adenosine with an inhibitory potassium current. this response disappears during whole-cell patch-clamp recording in which the cell is filled with minimal saline. we have found that this loss of sensitivity to adenosine can be prevented by including 100 microm gtp in the patch electrode filling solution. gdp is less effective than gtp in supporting the adenosine response, while gmp has little, if any, effect. treatments known to inhibit ... | 1987 | 2822865 |
| synthesis of protein in intestinal cells exposed to cholera toxin. | the mechanism by which cyclic adenosine monophosphate (amp), formed by intestinal epithelial cells in response to cholera toxin, ultimately results in alterations in water and electrolyte transport is poorly understood. several studies have indicated that inhibitors of transcription or translation block much of the transport of ions and water in the intestine and edema formation in tissue elicited by cholera toxin. data presented in this study confirmed the inhibitory effects of cycloheximide on ... | 1987 | 2823275 |
| pulmonary complications of aids: henry ford hospital experience between january 1984 and july 1986. | | 1987 | 2824409 |