| the diffusible factor synthase xanb2 is a bifunctional chorismatase that links the shikimate pathway to ubiquinone and xanthomonadins biosynthetic pathways. | the diffusible factor synthase xanb2, originally identified in xanthomonas campestris pv. campestris (xcc), is highly conserved across a wide range of bacterial species, but its substrate and catalytic mechanism have not yet been investigated. here, we show that xanb2 is a unique bifunctional chorismatase that hydrolyses chorismate, the end-product of the shikimate pathway, to produce 3-hydroxybenzoic acid (3-hba) and 4-hba. 3-hba and 4-hba are respectively associated with the yellow pigment xan ... | 2013 | 23113660 |
| xanthan chain length is modulated by increasing the availability of the polysaccharide copolymerase protein gumc and the outer membrane polysaccharide export protein gumb. | xanthan is a polysaccharide secreted by xanthomonas campestris that contains pentameric repeat units. the biosynthesis of xanthan involves an operon composed of 12 genes (gumb to gumm). in this study, we analyzed the proteins encoded by gumb and gumc. membrane fractionation showed that gumb was mainly associated with the outer membrane, whereas gumc was an inner membrane protein. by in silico analysis and specific globomycin inhibition, gumb was characterized as a lipoprotein. by reporter enzyme ... | 2013 | 23089617 |
| xanthomonas campestris pv. campestris (cause of black rot of crucifers) in the genomic era is still a worldwide threat to brassica crops. | xanthomonas campestris pv. campestris (xcc) (pammel) dowson is a gram-negative bacterium that causes black rot, the most important disease of vegetable brassica crops worldwide. intensive molecular investigation of xcc is gaining momentum and several whole genome sequences are available. | 2013 | 23051837 |
| the effect of hydrodynamic stress on the growth of xanthomonas campestris cultures in a stirred and sparged tank bioreactor. | the specific growth and the xanthan production rates by the bacterium xanthomonas campestris under different shear levels in shake flasks and in a stirred and sparged tank bioreactor have been studied. the shake flask has been used as a reference for studying the shear effects. an effectiveness factor expressed by the ratio of the observed growth rate and the growth rate without oxygen limitation or cell damage was calculated in both modes of cultures. it was observed that the effectiveness fact ... | 2013 | 23010723 |
| structural polymorphism of c-di-gmp bound to an eal domain and in complex with a type ii pilz-domain protein. | cyclic di-gmp (c-di-gmp) is a novel secondary-messenger molecule that is involved in regulating a plethora of important bacterial activities through binding to an unprecedented array of effectors. proteins with a canonical pilz domain that bind c-di-gmp play crucial roles in regulating flagellum-based motility. in contrast, noncanonical type ii pilz domains that do not effectively bind c-di-gmp regulate twitching motility, which is dependent on type iv pili (t4p). recent data indicate that t4p b ... | 2012 | 22993092 |
| dynamic complex formation between hd-gyp, ggdef and pilz domain proteins regulates motility in xanthomonas campestris. | rpfg is a member of a class of wide spread bacterial two-component regulators with an hd-gyp cyclic di-gmp phosphodiesterase domain. in the plant pathogen xanthomonas campestris, rpfg together with the sensor kinase rpfc regulates multiple factors as a response to the cell-to-cell diffusible signalling factor (dsf). a dynamic physical interaction of rpfg with two diguanylate cyclase (ggdef) domain proteins controls motility. here we show that, contrary to expectation, regulation of motility by t ... | 2012 | 22924852 |
| cefoxitin is both an inhibitor of class a beta-lactamase of xanthomonas campestris pv. campestris str. 17 and an inducer of its gene. | in xanthomonas campestris pv. campestris (xcc), the chromosomally encoded class a β-lactamase (bla(xcc)) is expressed at a high basal level in the absence of an inducer and its expression is inducible by ampicillin. like most of the class a β-lactamases, bla(xcc) cannot digest the β-lactam ring of cefoxitin. however, xcc exhibits high basal resistance to cefoxitin. a promoter activity assay with p(blaxcc)-lacz transcriptional fusion from a plasmid and western blotting using anti-bla(xcc) polyclo ... | 2013 | 22910283 |
| xanthan production by xanthomonas campestris using whey permeate medium. | xanthan gum is a polysaccharide that is widely used as stabilizer and thickener with many industrial applications in food industry. our aim was to estimate the ability of xanthomonas campestris atcc 13951 for the production of xanthan gum by using whey as a growth medium, a by-product of dairy industry. x. campestris atcc 13951 has been studied in batch cultures using a complex medium for the determination of the optimal concentration of glucose, galactose and lactose. in addition, whey was used ... | 2012 | 22806202 |
| analysis of new type iii effectors from xanthomonas uncovers xopb and xops as suppressors of plant immunity. | the pathogenicity of the gram-negative plant-pathogenic bacterium xanthomonas campestris pv. vesicatoria (xcv) is dependent on type iii effectors (t3es) that are injected into plant cells by a type iii secretion system and interfere with cellular processes to the benefit of the pathogen. in this study, we analyzed eight t3es from xcv strain 85-10, six of which were newly identified effectors. genetic studies and protoplast expression assays revealed that xopb and xops contribute to disease sympt ... | 2012 | 22738163 |
| the pepper rna-binding protein carbp1 functions in hypersensitive cell death and defense signaling in the cytoplasm. | the regulation of gene expression via post-transcriptional modification by rna-binding proteins is crucial for plant disease and innate immunity. here, we report the identification of the pepper (capsicum annuum) rna-binding protein1 gene (carbp1) as essential for hypersensitive cell death and defense signaling in the cytoplasm. carbp1 contains an rna recognition motif and is rapidly and strongly induced in pepper by avirulent xanthomonas campestris pv. vesicatoria (xcv) infection. carbp1 displa ... | 2012 | 22640562 |
| a xanthomonas uridine 5'-monophosphate transferase inhibits plant immune kinases. | plant innate immunity is activated on the detection of pathogen-associated molecular patterns (pamps) at the cell surface, or of pathogen effector proteins inside the plant cell. together, pamp-triggered immunity and effector-triggered immunity constitute powerful defences against various phytopathogens. pathogenic bacteria inject a variety of effector proteins into the host cell to assist infection or propagation. a number of effector proteins have been shown to inhibit plant immunity, but the ... | 2012 | 22504181 |
| improved thermostability of aeh by combining b-fit analysis and structure-guided consensus method. | α-amino ester hydrolases (aeh, e.c. 3.1.1.43) catalyze the synthesis and hydrolysis of α-amino β-lactam antibiotics. the aeh enzymes have been shown to feature excellent synthetic capability but suffer from poor thermostability. aeh from xanthomonas campestris exhibits an optimal activity temperature of 25 °c, an observed half-life of 5 min at 30 °c, and a "t-50" value, the temperature at which the half-life is 30 min, of 27 °c. to improve the thermostability of aeh, a modified structure-guided ... | 2012 | 22426092 |
| differential expression of peach erf transcriptional activators in response to signaling molecules and inoculation with xanthomonas campestris pv. pruni. | ethylene response factors (erfs) are a large family of transcription factors (tfs) that have diverse functions in plant development and immunity. however, very little is known about the molecular regulation of these tfs in stone fruits during disease incidence. in the present study, we describe the identification of five peach erfs (pp-erfs), aiming to elucidate their potential roles in defense against xanthomonas campestris pv. pruni (xcp), the causal agent of bacterial spot disease. the phylog ... | 2012 | 22410465 |
| characterization of hrpb2 from xanthomonas campestris pv. vesicatoria identifies protein regions that are essential for type iii secretion pilus formation. | the gram-negative plant-pathogenic bacterium xanthomonas campestris pv. vesicatoria employs a type iii secretion (t3s) system to translocate effector proteins into plant cells. t3s depends on hrpb2, which is essential for assembly of the extracellular t3s pilus and is itself weakly secreted. to characterize the role of hrpb2, we used a transposon mutagenesis approach, which led to the insertion of pentapeptide-encoding sequences into hrpb2. complementation studies with hrpb2 mutant derivatives r ... | 2012 | 22343358 |
| interspecific hybridization of cultivated rice, oryza sativa l. with the wild rice, o. minuta presl. | crosses were made between four varieties ('mahsuri', 'setanjung", 'mr84" and 'mr103") of oryza sativa l. (2n=24, aa) and one accession of o. minuta (2n= 8, bbcc). the seed set obtained ranged between 9.5% and 25.1% depending on the rice variety used. by rescuing 14-day-old embryos and culturing them on 25%-strength ms medium we obtained a total of 414 f1 hybrids. the f1s were vigorous, tillered profusely, were perennial and male-sterile. the hybrids were triploid (abc) with 36 chromosomes and sh ... | 1996 | 24162392 |
| transfer of resistance to xanthomonas campestris pv campestris into brassica oleracea l. by protoplast fusion. | black rot caused by the bacterium xanthomonas campestris pv campestris is one of the most serious diseases of brassica oleracea. since sources of resistance to the disease within b. oleracea are insufficient and control means are limited, the development of resistant breeding lines is extremely desirable. certain lines of b. napus contain very high resistance controlled by a dominant gene, but crossing the two species sexually is very difficult. therefore, somatic hybrids were produced by protop ... | 1995 | 24170061 |
| xanthan gum production by wild-type isolates of xanthomonas campestris. | five newly-isolated strains of xanthomonas campestris when compared with the standard strain, nrrl b-1459, showed higher broth viscosity and xanthan gum production. evaluation of polysaccharide rheology is a very important determinant for selecting new xanthan-producing isolates. | 1995 | 24414901 |
| screening, isolation, and identification of food-originated compounds enhancing the ice-nucleation activity of xanthomonas campestris. | some foods from plant sources, particularly of moringeceae origin, contained compounds that enhanced bacterial ice-nucleation activity. a hot water extract from mustard seeds was so potent in its enhancing activity that the compounds responsible could be isolated from the seeds as yellow crystals. by instrumental analyses, this enhancer was identified as 4-hydroxy-3-nitrophenylacetic acid. the addition of a purified preparation of this compound at 0.1-1 ppm to the cultivation medium for xanthomo ... | 1994 | 27315706 |
| regulatory mutations affecting the synthesis of pectate lyase in xanthomonas campestris. | four classes of xanthomonas campestris mutants were identified with respect to pectate lyase. pectate lyase production in the wild-type and classes i and iib mutants was partially dependent on the growth-phase whereas in classes iia and iii it was totally dependent. enzyme activity in some of the mutants was constitutive and resistant to catabolite repression. | 1993 | 24419956 |
| high-pressure sterilization of ice nucleation-active xanthomonas campestris and its application to egg processing. | xanthomonas campestris inxc-1 is able to freeze water at a subzero temperature higher than -5°c. high-pressure treatment at 300 mpa and 5°c for 5 min killed the cells without affecting their ice-nucleation activity. egg white containing the pressurized cells began to freeze with a slight degree of supercooling. ice crystals with a dendritic structure were formed when the egg white froze in the presence of the killed bacterial cells. the frozen egg white thawed more quickly than egg white frozen ... | 1993 | 27280991 |
| quantitative analysis of resistance in cotton to three new isolates of the bacterial blight pathogen. | genetic variability for virulence of the bacterial blight pathogen [xanthomonas campestris pv malvacearum (smith) dye] on cotton (gossypium hirsutum l.) has been shown by the identification of 19 races of the pathogen based on disease reactions of a set of ten host differentials. this study was conducted to determine the inheritance of host resistance to three recently identified isolates of x. campestris pv malvacearum, which are virulent on the entire set of differentials. true leaves of tamco ... | 1990 | 24226445 |
| selection of peach cells for insensitivity to culture filtrates of xanthomonas campestris pv. pruni and regeneration of resistant plants. | individual callus cultures were initiated from 400 immature embryos of bacterial leaf spot-susceptible 'sunhigh' peach. each was subjected to several selection cycles of a toxic culture filtrate produced by xanthomonas campestris pv. pruni, the causal agent of leaf spot of peach. progressively higher concentrations of the filtrate were used in each cycle. two calli survived, and two plants were regenerated from each of the surviving calli. each of the four clones was propagated in vitro and test ... | 1988 | 24232397 |
| gene expression in brassica campestris showing a hypersensitive response to the incompatible pathogen xanthomonas campestris pv. vitians. | xanthomonas campestris pv. vitians, a pathogen of lettuce, elicits a hypersensitive response within 12 hours of inoculation into brassica leaves, characterized by tissue collapse, loss of membrane integrity, vein blockage and melanin production. in contrast, the compatible pathogen, x. c. pv. campestris, has no visible effects on leaves for 48 hours, after which inoculated areas show chlorosis which eventually spreads, followed by rotting.mrna was prepared from leaves inoculated with suspensions ... | 1987 | 24301262 |
| internalisation of escherichia coli o157:h7 gfp+ in rocket and swiss chard baby leaves as affected by abiotic and biotic damage. | internalisation of human pathogens in edible parts of vegetables eaten raw is a major concern, since once internalised they are protected from sanitising treatments. in this study, we examined invasion of gfp-labelled e. coli o157:h7 into intact and biotically (infection with xanthomonas campestris/pseudomonas syringae) and abiotically (grating with silicon carbide) damaged leaves of wild rocket (diplotaxis tenuifolia) and swiss chard (beta vulgaris subsp. cicla) using laser scanning confocal mi ... | 2017 | 28397273 |
| screening of leaves extracts from calamus aromaticus for their antimicrobial activity by disc diffusion assay. | the current research investigates the anti-microbial activities of different solvent extracted samples from the leaves of calamus aromaticus against gram positive, gram negative bacteria and fungi using 500, 1000 and 2000 μg disc(-1) concentrations. escherichia coli, citorbacter freundii and candida albicans showed resistivity to crude methanolic extract and the same microbes were more susceptible to water extracted fractions. maximum activity was measured by hexane extracted fractions against p ... | 2017 | 28653924 |
| dissecting virulence function from recognition: cell death suppression in nicotiana benthamiana by xopq/hopq1-family effectors relies on eds1-dependent immunity. | many gram-negative plant pathogenic bacteria express effector proteins of the xopq/hopq1 family which are translocated into plant cells via the type iii secretion system during infection. in nicotiana benthamiana, recognition of xopq/hopq1 proteins induces an effector-triggered immunity (eti) reaction which is not associated with strong cell death but renders plants immune against pseudomonas syringae and xanthomonas campestris pv. vesicatoria strains. additionally, xopq suppresses cell death in ... | 2017 | 28423458 |
| bacterial diseases of bananas and enset: current state of knowledge and integrated approaches toward sustainable management. | bacterial diseases of bananas and enset have not received, until recently, an equal amount of attention compared to other major threats to banana production such as the fungal diseases black leaf streak (mycosphaerella fijiensis) and fusarium wilt (fusarium oxysporum f. sp. cubense). however, bacteria cause significant impacts on bananas globally and management practices are not always well known or adopted by farmers. bacterial diseases in bananas and enset can be divided into three groups: (1) ... | 2017 | 28785275 |
| 1-methoxypyrrole-2-carboxamide-a new pyrrole compound isolated from streptomyces griseocarneus sww368. | a new pyrrole compound, 1-methoxypyrrole-2-carboxamide, was obtained from a culture broth of streptomyces griseocarneus sww368, which was isolated from the rhizospheric soil under a para rubber tree (hevea brasiliensis). the chemical structure was elucidated by 1d nmr, 2d nmr, and ms, as a pyrrole ring with a n-methoxy group and a primary amide group. it exhibited antibacterial properties against kocuria rhizophila, staphylococcus aureus and xanthomonas campestris pv. oryzae; however, cytotoxici ... | 2017 | 28502957 |
| an essential regulatory system originating from polygenic transcriptional rewiring of phop-phoq of xanthomonas campestris. | how essential, regulatory genes originate and evolve is intriguing because mutations of these genes not only lead to lethality in organisms, but also have pleiotropic effects since they control the expression of multiple downstream genes. therefore, the evolution of essential, regulatory genes is not only determined by genetic variations of their own sequences, but also by the biological function of downstream genes and molecular mechanisms of regulation. to understand the origin of essential, r ... | 2017 | 28550013 |
| analysis of the zar1 immune complex reveals determinants for immunity and molecular interactions. | plants depend on innate immunity to prevent disease. plant pathogenic bacteria, like pseudomonas syringae and xanthomonas campestris, use the type iii secretion system as a molecular syringe to inject type iii secreted effector (t3se) proteins in plants. the primary function of most t3ses is to suppress immunity; however, the plant can evolve nucleotide-binding domain-leucine-rich repeat domain-containing proteins to recognize specific t3ses. the atzar1 nlr induces strong defense responses again ... | 2017 | 28652264 |
| hpab-dependent secretion of type iii effectors in the plant pathogens ralstonia solanacearum and xanthomonas campestris pv. vesicatoria. | plant pathogenic bacteria exerts their pathogenicity through the injection of large repertoires of type iii effectors (t3es) into plant cells, a mechanism controlled in part by type iii chaperones (t3cs). in ralstonia solanacearum, the causal agent of bacterial wilt, little is known about the control of type iii secretion at the post-translational level. here, we provide evidence that the hpab and hpad proteins do act as bona fide r. solanacearum class ib chaperones that associate with several t ... | 2017 | 28687734 |
| an efficient approach to explore and discriminate anomalous regions in bacterial genomes based on maximum entropy. | recently, there has been an increase in the number of whole bacterial genomes sequenced, mainly due to the advancing of next-generation sequencing technologies. in face of this, there is a need to provide new analytical alternatives that can follow this advance. given our current knowledge about the genomic plasticity of bacteria and that those genomic regions can uncover important features about this microorganism, our goal was to develop a fast methodology based on maximum entropy (me) to guid ... | 2017 | 28570142 |
| xcc2366, a gene encoding a putative tetr family transcriptional regulator, is required for acriflavin resistance and virulence of xanthomonas campestris pv. campestris. | xanthomonas campestris pv. campestris (xcc) is the phytopathogen that causes black rot disease in cruciferous plants. the xcc2366 gene product is annotated as a protein belonging to the tetr family of transcriptional regulators. in this study, we evaluated the function and expression of the xcc2366 gene. mutational analysis demonstrated that xcc2366 is involved in the resistance to acriflavin and is necessary for virulence in xcc. in addition, the xcc2366 transcription initiation site was mapped ... | 2017 | 28821943 |
| loss of chloroplast localized protein phosphatase 2cs in arabidopsis thaliana leads to enhancement of the plant immunity and resistance to xanthomonas campestris pv. campestris infection. | protein phosphatases (pps) counteract kinases in reversible phosphorylation events during numerous signal transduction pathways in eukaryotes. pp2cs, one of the four major classes of serine/threonine specific pps family is greatly expanded in plants. thus pp2c is thought to have a specific role in a signal transduction pathway. some rice pp2cs classified to subgroup k were responsive to the infection by the compatible xanthomonas oryzae pv. oryzae, the causal agent of bacterial blight. in a. tha ... | 2017 | 28815858 |
| systems and synthetic biology perspective of the versatile plant-pathogenic and polysaccharide-producing bacterium xanthomonas campestris. | bacteria of the genus xanthomonas are a major group of plant pathogens. they are hazardous to important crops and closely related to human pathogens. being collectively a major focus of molecular phytopathology, an increasing number of diverse and intricate mechanisms are emerging by which they communicate, interfere with host signalling and keep competition at bay. interestingly, they are also biotechnologically relevant polysaccharide producers. systems biotechnology techniques have revealed t ... | 2017 | 28795660 |
| retraction for ryan et al., cell-cell signaling in xanthomonas campestris involves an hd-gyp domain protein that functions in cyclic di-gmp turnover. | | 2017 | 28784774 |
| the type iii secretion chaperone hpab controls the translocation of effector and non-effector proteins from xanthomonas campestris pv. vesicatoria. | pathogenicity of the gram-negative bacterium xanthomonas campestris pv. vesicatoria depends on a type iii secretion (t3s) system, which translocates effector proteins into plant cells. effector proteins contain n-terminal t3s and translocation signals and interact with the t3s chaperone hpab, which presumably escorts effectors to the secretion apparatus. the molecular mechanisms underlying the recognition of effectors by the t3s system are not yet understood. in the present study, we analysed t3 ... | 2017 | 28771395 |
| introgression of black rot resistance from brassica carinata to cauliflower (brassica oleracea botrytis group) through embryo rescue. | black rot caused by xanthomonas campestris pv. campestris (xcc) is a very important disease of cauliflower (brassica oleracea botrytis group) resulting into 10-50% yield losses every year. since there is a dearth of availability of resistance to black rot disease in b. oleracea (c genome), therefore exploration of a and b genomes was inevitable as they have been reported to be potential reservoirs of gene(s) for resistance to black rot. to utilize these sources, interspecific hybrid and backcros ... | 2017 | 28769959 |
| direct quantitative evaluation of disease symptoms on living plant leaves growing under natural light. | leaf color is an important indicator when evaluating plant growth and responses to biotic/abiotic stress. acquisition of images by digital cameras allows analysis and long-term storage of the acquired images. however, under field conditions, where light intensity can fluctuate and other factors (shade, reflection, and background, etc.) vary, stable and reproducible measurement and quantification of leaf color are hard to achieve. digital scanners provide fixed conditions for obtaining image data ... | 2017 | 28744185 |
| chloroplast redox status modulates genome-wide plant responses during the non-host interaction of tobacco with the hemibiotrophic bacterium xanthomonas campestris pv. vesicatoria. | non-host resistance is the most ample and durable form of plant resistance against pathogen infection. it includes induction of defense-associated genes, massive metabolic reprogramming, and in many instances, a form of localized cell death (lcd) at the site of infection, purportedly designed to limit the spread of biotrophic and hemibiotrophic microorganisms. reactive oxygen species (ros) have been proposed to act as signals for lcd orchestration. they are produced in various cellular compartme ... | 2017 | 28725231 |
| combined biotic stresses trigger similar transcriptomic responses but contrasting resistance against a chewing herbivore in brassica nigra. | in nature, plants are frequently exposed to simultaneous biotic stresses that activate distinct and often antagonistic defense signaling pathways. how plants integrate this information and whether they prioritize one stress over the other is not well understood. | 2017 | 28716054 |
| genetically engineered bananas resistant to xanthomonas wilt disease and nematodes. | banana is an important staple food crop feeding more than 100 million africans, but is subject to severe productivity constraints due to a range of pests and diseases. banana xanthomonas wilt caused by xanthomonas campestris pv. musacearum is capable of entirely destroying a plantation while nematodes can cause losses up to 50% and increase susceptibility to other pests and diseases. development of improved varieties of banana is fundamental in order to tackle these challenges. however, the ster ... | 2017 | 28713567 |
| xanthan gum production using jackfruit-seed-powder-based medium: optimization and characterization. | xanthan gum (xg) production by xanthomonas campestris ncim 2961 using jackfruit seed powder (jsp) as a novel substrate was reported. central composite design (ccd) of response surface method (rsm) was used to evaluate the linear and interaction effects of five medium variables (jsp, peptone, citric acid, k2hpo4 and kh2po4) for xg production. maximum xg production (51.62 g/l) was observed at the optimum level of jsp (4 g/l), peptone (0.93 g/l), citric acid (0.26 g/l), k2hpo4 (1.29 g/l) and kh2po4 ... | 2017 | 28711983 |
| xanthomonas campestris pv. musacearum: a major constraint to banana, plantain and enset production in central and east africa over the past decade. | taxonomy: bacteria; phylum proteobacteria; class gammaproteobacteria; order xanthomonadales; family xanthomonadaceae; genus xanthomonas; currently classified as x. campestris pv. musacearum (xcm). however, fatty acid methyl esters analysis, genetic and genomic evidence suggest that this pathogen is x. vasicola and resides in a separate pathovar. isolation and detection: xcm can be isolated on yeast peptone glucose agar (ypga), cellobiose cephalexin agar and yeast tryptone sucrose agar (ytsa) com ... | 2017 | 28677256 |
| xc_0531 encodes a c-type cytochrome biogenesis protein and is required for pathogenesis in xanthomonas campestris pv. campestris. | the phytopathogenic xanthomonas campestris pv.campestris is a gram-negative bacterium and the causal agent of black-rot disease of cruciferous crops. many gram-negative bacteria possess a family of proteins, called dsbs, which are involved in disulfide bond formation in certain periplasmic proteins. in our preliminary screening of the virulence to the plants we identified that gene xc_0531 which annotated gene dsbd of xanthomonas campestris pv. campestris (xcc) is related to the virulence to the ... | 2017 | 28655353 |
| a robust protocol for the isolation of cellular proteins from xanthomonas campestris to analyze the effect of methionine on xanthan production in 2d-gel experiments. | two-dimensional page (2d-page) is a key technique for the separation of complex protein samples to survey the protein inventory of prokaryotic microorganisms. although the preparation of proteins is a critical step in 2d gel experiments, its effect on the outcome of proteome data is often underestimated. in this work, we show that the choice of protein isolation method can have a profound impact on the quality of 2d gels of protein extracts from prokaryotes. based on xanthomonas campestris, a co ... | 2017 | 28605028 |
| functional roles of the pepper leucine-rich repeat protein and its interactions with pathogenesis-related and hypersensitive-induced proteins in plant cell death and immunity. | pepper leucine-rich repeat protein (calrr1) interacts with defense response proteins to regulate plant cell death and immunity. this review highlights the current understanding of the molecular functions of calrr1 and its interactor proteins. plant cell death and immune responses to microbial pathogens are controlled by complex and tightly regulated molecular signaling networks. xanthomonas campestris pv. vesicatoria (xcv)-inducible pepper (capsicum annuum) leucine-rich repeat protein 1 (calrr1) ... | 2017 | 28508261 |
| refined annotation of the complete genome of the phytopathogenic and xanthan producing xanthomonas campestris pv. campestris strain b100 based on rna sequence data. | bioinformatics tools and gene expression data were applied to identify new genes and to enhance the accuracy in genomic feature predictions for xanthomonas campestris pv. campestris (xcc) b100, a pathogen of cruciferous plants and model strain for the biosynthesis of xanthan, a polysaccharide with a multitude of commercial applications as a thickening agent. results from 5'-enriched end rna sequencing (rna-seq) and total transcriptome rna-seq experiments were used for this purpose. functional ge ... | 2017 | 28506932 |
| time-resolved energetics of photoprocesses in prokaryotic phytochrome-related photoreceptors. | time-resolved photoacoustics (pa) is uniquely able to explore the energy landscape of photoactive proteins and concomitantly detects light-induced volumetric changes (δv) accompanying the formation and decay of transient species in a time window between ca. 20 ns and 5 μs. here, we report pa measurements on diverse photochromic bilin-binding photoreceptors of prokaryotic origin: (1) the chromophore-binding gaf3 domain of the red (r)/green (g) switching cyanobacteriochrome 1393 (slr1393g3) from s ... | 2017 | 28500712 |
| cyclic di-gmp signalling in the virulence and environmental adaptation of xanthomonas campestris. | | 2017 | 28493340 |
| differential accumulation of xanthomonas campestris pv. campestris proteins during the interaction with the host plant: contributions of an in vivo system. | xanthomonas campestris pv. campestris (xcc) is the causal agent of black rot, a highly destructive disease that affects all brassicas. this work aimed to study the interaction xcc-brassica oleracea using an in vivo system in an attempt to identify proteins involved in pathogenicity. we used label-free shotgun 2d-nanouplc/ms(e) to analyze xcc proteins in three conditions: in the interaction with susceptible (rek) and resistant (reu) plants and in culture medium (control condition). a model of xcc ... | 2017 | 28471538 |
| retraction statement: dynamic complex formation between hd-gyp, ggdef and pilz domain proteins regulates motility in xanthomonas campestris. | the following article from molecular microbiology (2012) 86(3), 557-567, 'dynamic complex formation between hd-gyp, ggdef and pilz domain proteins regulates motility in xanthomonas campestris' by robert p. ryan, yvonne mccarthy, patrick a. kiely, rosemary o'connor, chuck s. farah, judith p. armitage and j. maxwell dow published online in wiley online library (wileyonlinelibrary.com), has been retracted by agreement between the authors, the journal editor-in-chief, john d helmann, and john wiley ... | 2017 | 28421674 |