| stability of sars coronavirus in human specimens and environment and its sensitivity to heating and uv irradiation. | the causal agent for sars is considered as a novel coronavirus that has never been described both in human and animals previously. the stability of sars coronavirus in human specimens and in environments was studied. | 2003 | 14631830 |
| implications of sars: medical geography and surveillance in disease detection. | to expose pharmacy educators and practitioners to concepts of medical geography and medical surveillance. severe acute respiratory syndrome (sars) is used as a case example because it is an emerging infection and a prime example of the type of disease that pharmacists may encounter in daily practice (e.g., easily transmitted, resembles the common cold). | 2003 | 14632586 |
| potential of zoonotic transmission of non-primate foamy viruses to humans. | the zoonotic introduction of an animal pathogen into the human population and the subsequent extension or alteration of its host range leading to the successful maintenance of the corresponding pathogen by human-to-human transmission pose a serious risk for world-wide health care. such a scenario occurred for instance by the introduction of simian immunodeficiency viruses into the human population resulting in the human immunodeficiency viruses (hiv) and the subsequent aids pandemic or the propo ... | 2003 | 14633194 |
| [the quantitative detection of anti-coronavirus antibody titer in medical personnel closely contacted with severe acute respiratory syndrome patients]. | to study the serum anti-coronavirus antibody titer in medical personnel who had closely contacted with severe acute respiratory syndrome (sars) patients. | 2003 | 14633437 |
| [dynamic changes and the meanings of blood cytokines in severe acute respiratory syndrome]. | to investigate the dynamic changes observed in serum levels of interleukins (ils), tumor necrosis factor-alpha (tnf-alpha) and transforming growth factor-beta1 (tgf-beta1) in severe acute respiratory syndrome (sars). | 2003 | 14633438 |
| [the first case of severe acute respiratory syndrome in foshan]. | to evaluate the epidemiologic feature, diagnosis and treatment of severe acute respiratory syndrome (sars). | 2003 | 14633441 |
| sars--unprecedented global response to a newly emerging disease. | | 2003 | 14503787 |
| [israeli guidelines for investigation and management of sars cases and their contacts]. | as the first emerging epidemic of the 21st century, sars has raised much interest and fear among statesmen, economists, and the general public, as well as within the medical community. this new disease is characterized by several daunting challenges, including person-to-person transmission, an environmentally resistant agent, a non-specific clinical presentation, and a high fatality rate among high risk individuals. in israel, as elsewhere, public health authorities were forced to quickly design ... | 2003 | 14518156 |
| early diagnosis of sars coronavirus infection by real time rt-pcr. | a novel coronavirus was recently identified as the aetiological agent of severe acute respiratory syndrome (sars). molecular assays currently available for detection of sars-coronavirus (sars-cov) have low sensitivity during the early stage of the illness. | 2003 | 14522060 |
| serial analysis of the plasma concentration of sars coronavirus rna in pediatric patients with severe acute respiratory syndrome. | | 2003 | 14633884 |
| detection of sars coronavirus rna in the cerebrospinal fluid of a patient with severe acute respiratory syndrome. | | 2003 | 14633896 |
| sars infection among health care workers in beijing, china. | | 2003 | 14645305 |
| inhibition of sars-associated coronavirus infection and replication by rna interference. | | 2003 | 14645307 |
| phylogeny of the sars coronavirus. | | 2003 | 14645828 |
| reverse genetics with a full-length infectious cdna of severe acute respiratory syndrome coronavirus. | a previously undescribed coronavirus (cov) is the etiologic agent responsible for severe acute respiratory syndrome (sars). using a panel of contiguous cdnas that span the entire genome, we have assembled a full-length cdna of the sars-cov urbani strain, and have rescued molecularly cloned sars viruses (infectious clone sars-cov) that contained the expected marker mutations inserted into the component clones. recombinant viruses replicated as efficiently as wt virus and both were inhibited by tr ... | 2003 | 14569023 |
| angiotensin-converting enzyme 2 is a functional receptor for the sars coronavirus. | spike (s) proteins of coronaviruses, including the coronavirus that causes severe acute respiratory syndrome (sars), associate with cellular receptors to mediate infection of their target cells. here we identify a metallopeptidase, angiotensin-converting enzyme 2 (ace2), isolated from sars coronavirus (sars-cov)-permissive vero e6 cells, that efficiently binds the s1 domain of the sars-cov s protein. we found that a soluble form of ace2, but not of the related enzyme ace1, blocked association of ... | 2003 | 14647384 |
| [sars-cov: 2. modeling sars epidemic]. | | 2003 | 14648488 |
| report details lessons from sars outbreak. | | 2003 | 14570038 |
| studies explore impact of new pathogens: investigators report on metapneumovirus, sars. | | 2003 | 14570932 |
| prediction of proteinase cleavage sites in polyproteins of coronaviruses and its applications in analyzing sars-cov genomes. | recently, we have developed a coronavirus-specific gene-finding system, zcurve_cov 1.0. in this paper, the system is further improved by taking the prediction of cleavage sites of viral proteinases in polyproteins into account. the cleavage sites of the 3c-like proteinase and papain-like proteinase are highly conserved. based on the method of traditional positional weight matrix trained by the peptides around cleavage sites, the present method also sufficiently considers the length conservation ... | 2003 | 14572668 |
| [analysis of false-positive associated with antibody tests for sars-cov in sle patients]. | to discuss the false-positive of serological diagnostic testing for coronavirus antibody in patients with systemic lupus erythematosus(sle), 66 normal individual and 31 sle with non-sars patients were detected for sars-associated coronavirus (sars-cov) antibody and rna by enzymelinked immunosorbent assays(elisa) and reverse transcriptase-polymerase chain reaction(rt-pcr). the result showed 2/66 cases(3.0%) were positive of sars-cov-igg antibody and 66 cases were negative of sars-cov-igm antibody ... | 2003 | 14574997 |
| [genomic variations in the locus for aminopeptidase n:a putative cellular receptor for sars-cov spike glycoprotein]. | aminopeptidase n has been identified as the cellular receptor for human coronavirus hcov-229e and was a putative receptor for the spike glycoprotein encoded by the sars-associated coronavirus (sars-cov). we report here identification of 9 single nucleotide polymorphisms (snps) in anpep, encoding human aminopeptidase n, in chinese. all anpep exons and their flanking intronic sequences were amplified from unrelated normal individuals by polymerase chain reaction (pcr) and screened using denaturing ... | 2003 | 14579541 |
| the crystal structures of severe acute respiratory syndrome virus main protease and its complex with an inhibitor. | a newly identified severe acute respiratory syndrome coronavirus (sars-cov), is the etiological agent responsible for the outbreak of sars. the sars-cov main protease, which is a 33.8-kda protease (also called the 3c-like protease), plays a pivotal role in mediating viral replication and transcription functions through extensive proteolytic processing of two replicase polyproteins, pp1a (486 kda) and pp1ab (790 kda). here, we report the crystal structures of the sars-cov main protease at differe ... | 2003 | 14585926 |
| identifying inhibitors of the sars coronavirus proteinase. | the severe acute respiratory syndrome (sars) is a serious respiratory illness that has recently been reported in parts of asia and canada. in this study, we use molecular dynamics (md) simulations and docking techniques to screen 29 approved and experimental drugs against the theoretical model of the sars cov proteinase as well as the experimental structure of the transmissible gastroenteritis virus (tgev) proteinase. our predictions indicate that existing hiv-1 protease inhibitors, l-700,417 fo ... | 2003 | 14592491 |
| molecular diagnostics of atypical pneumonia. | the emergence of nucleic acid-based molecular techniques has significantly enhanced laboratory diagnosis and monitoring of atypical pneumonia. these techniques have not only provided rapid and sensitive detection of fastidious microbial organisms but have also played critical roles in identifying and characterizing emerging pathogens that cause atypical pneumonia. other benefits that molecular techniques can bring to the field include organism differentiation, quantitation, typing, and antibioti ... | 2003 | 14653964 |
| infectious diseases. search for sars origins stalls. | | 2003 | 14593142 |
| molecular model of sars coronavirus polymerase: implications for biochemical functions and drug design. | the causative agent of severe acute respiratory syndrome (sars) is a previously unidentified coronavirus, sars-cov. the rna-dependent rna polymerase (rdrp) of sars-cov plays a pivotal role in viral replication and is a potential target for anti-sars therapy. there is a lack of structural or biochemical data on any coronavirus polymerase. to provide insights into the structure and function of sars-cov rdrp, we have located its conserved motifs that are shared by all rdrps, and built a three-dimen ... | 2003 | 14654687 |
| infectious diseases. unexplained false alarm may hold lessons. | | 2003 | 14593143 |
| implications of unrecognized severe acute respiratory syndrome. | | 2003 | 14657739 |
| biosynthesis, purification, and substrate specificity of severe acute respiratory syndrome coronavirus 3c-like proteinase. | the 3c-like proteinase of severe acute respiratory syndrome (sars) coronavirus has been proposed to be a key target for structural-based drug design against sars. in order to understand the active form and the substrate specificity of the enzyme, we have cloned, expressed, and purified sars 3c-like proteinase. analytic gel filtration shows a mixture of monomer and dimer at a protein concentration of 4 mg/ml and mostly monomer at 0.2 mg/ml, which correspond to the concentration used in the enzyme ... | 2004 | 14561748 |
| emerging viral infections in a rapidly changing world. | emerging viral infections in both humans and animals have been reported with increased frequency in recent years. recent advances have been made in our knowledge of some of these, including severe acute respiratory syndrome-associated coronavirus, influenza a virus, human metapneumovirus, west nile virus and ebola virus. research efforts to mitigate their effects have concentrated on improved surveillance and diagnostic capabilities, as well as on the development of vaccines and antiviral agents ... | 2003 | 14662395 |
| [sars-cov: 1. the virus]. | | 2003 | 14593622 |
| taiwanese scientists find genetic link to sars. | | 2003 | 14595411 |
| diagnosis of severe acute respiratory syndrome (sars) by detection of sars coronavirus nucleocapsid antibodies in an antigen-capturing enzyme-linked immunosorbent assay. | recombinant severe acute respiratory syndrome (sars) coronavirus nucleocapsid protein was employed to establish an antigen-capturing enzyme-linked immunosorbent assay (elisa). antinucleocapsid protein antibodies could be detected in 68.4% of probable sars patients 6 to 10 days after illness and in 89.6% of the patients 11 to 61 days after illness. no false-positive results were observed in 20 non-sars fever patients, 24 non-sars respiratory illness patients, and 20 health care workers. among 940 ... | 2003 | 14662982 |
| effects of a sars-associated coronavirus vaccine in monkeys. | the causative agent of severe acute respiratory syndrome (sars) has been identified as a new type of coronavirus. here, we have investigated the ability of adenoviral delivery of codon-optimised sars-cov strain urbani structural antigens spike protein s1 fragment, membrane protein, and nucleocapsid protein to induce virus-specific broad immunity in rhesus macaques. we immunised rhesus macaques intramuscularly with a combination of the three ad5-sars-cov vectors or a control vector and gave a boo ... | 2003 | 14667748 |
| severe acute respiratory syndrome and influenza: virus incursions from southern china. | | 2003 | 14668255 |
| revised u.s. surveillance case definition for severe acute respiratory syndrome (sars) and update on sars cases--united states and worldwide, december 2003. | during the 2003 epidemic of severe acute respiratory syndrome (sars), cdc and the council of state and territorial epidemiologists (cste) developed surveillance criteria to identify persons with sars. the surveillance case definition changed throughout the epidemic as understanding of the clinical, laboratory, and transmission characteristics of sars-associated coronavirus (sars-cov) increased. on june 26, cste adopted a position statement to add sars-cov disease to the national notifiable disea ... | 2003 | 14668711 |
| a 193-amino acid fragment of the sars coronavirus s protein efficiently binds angiotensin-converting enzyme 2. | the coronavirus spike (s) protein mediates infection of receptor-expressing host cells and is a critical target for antiviral neutralizing antibodies. angiotensin-converting enzyme 2 (ace2) is a functional receptor for the coronavirus (severe acute respiratory syndrome (sars)-cov) that causes sars. here we demonstrate that a 193-amino acid fragment of the s protein (residues 318-510) bound ace2 more efficiently than did the full s1 domain (residues 12-672). smaller s protein fragments, expressin ... | 2004 | 14670965 |
| mosaic evolution of the severe acute respiratory syndrome coronavirus. | severe acute respiratory syndrome (sars) is a deadly form of pneumonia caused by a novel coronavirus, a viral family responsible for mild respiratory tract infections in a wide variety of animals including humans, pigs, cows, mice, cats, and birds. analyses to date have been unable to identify the precise origin of the sars coronavirus. we used bayesian, neighbor-joining, and split decomposition phylogenetic techniques on the sars virus replicase, surface spike, matrix, and nucleocapsid proteins ... | 2004 | 14671089 |
| systematic, genome-wide identification of host genes affecting replication of a positive-strand rna virus. | positive-strand rna viruses are the largest virus class and include many pathogens such as hepatitis c virus and the severe acute respiratory syndrome coronavirus (sars). brome mosaic virus (bmv) is a representative positive-strand rna virus whose rna replication, gene expression, and encapsidation have been reproduced in the yeast saccharomyces cerevisiae. by using traditional yeast genetics, host genes have been identified that function in controlling bmv translation, selecting bmv rnas as rep ... | 2003 | 14671320 |
| [expression, purification and identification of recombinant sars coronavirus membrane protein]. | a novel coronavirus (sars-coronavirus, sars-cov) was discovered as the pathogen of the severe acute respiratory syndrome (sars). according to studies with other coronaviruses, the membrane protein (m protein) is the main structural protein and the recombinant m protein may be useful as an antigen for detecting antibodies against coronavirus and for preparing vaccine. in this work, the m protein of sars-cov was expressed in e. coli as fusion protein with maltose binding protein at n-terminus and ... | 2003 | 14673508 |
| severe acute respiratory distress syndrome (sars): a critical care perspective. | to review the epidemiology, clinical features, etiology, diagnosis, and management of severe acute respiratory syndrome (sars) from a critical care perspective. | 2003 | 14605542 |
| characterizing degradation products of peptides containing n-terminal cys residues by (off-line high-performance liquid chromatography)/matrix-assisted laser desorption/ionization quadrupole time-of-flight measurements. | a transformation analogous to the well-known conversion of an n-terminal glutamine residue to pyroglutamic acid is the cyclization of an n-terminal carboxyamidomethylated cysteine residue (the normal product of alkylation with iodoacetamide). this yields 5-oxothiomorpholine-3-carboxylic acid, with the same 17 da mass loss observed in the gln reaction. nineteen tryptic peptides with cys at the n-terminal were identified for this study, and compared with eight with n-terminal gln. when examined by ... | 2003 | 14608624 |
| severe acute respiratory syndrome vaccine development: experiences of vaccination against avian infectious bronchitis coronavirus. | vaccines against infectious bronchitis of chickens (gallus gallus domesticus) have arguably been the most successful, and certainly the most widely used, of vaccines for diseases caused by coronaviruses, the others being against bovine, canine, feline and porcine coronaviruses. infectious bronchitis virus (ibv), together with the genetically related coronaviruses of turkey (meleagris gallopovo) and ring-necked pheasant (phasianus colchicus), is a group 3 coronavirus, severe acute respiratory syn ... | 2003 | 14676007 |
| severe acute respiratory syndrome vaccine development: experiences of vaccination against avian infectious bronchitis coronavirus. | vaccines against infectious bronchitis of chickens (gallus gallus domesticus) have arguably been the most successful, and certainly the most widely used, of vaccines for diseases caused by coronaviruses, the others being against bovine, canine, feline and porcine coronaviruses. infectious bronchitis virus (ibv), together with the genetically related coronaviruses of turkey (meleagris gallopovo) and ring-necked pheasant (phasianus colchicus), is a group 3 coronavirus, severe acute respiratory syn ... | 2003 | 14676007 |
| sars. | | 2003 | 14677346 |
| a wake-up call for our health-care system. | | 2003 | 14677347 |
| emerging viruses set to soar. | the emergence rate of novel viruses, such as the coronavirus that sparked sars, could well be on the rise. researchers now think that the sars virus split from group 2 coronaviruses, and that this happened relatively recently on the scale of coronavirus evolution. | 2003 | 14678728 |
| [epidemiological study of the features of the index cases in sars case clusters]. | to understand the epidemiological characteristics of the index cases in severe acute respiratory syndrome (sars) cases clusters and to evaluate their importance in the whole epidemic process of sars. | 2003 | 14678890 |
| assessment of putative protein targets derived from the sars genome. | the ability to rapidly and reliably develop hypotheses on the function of newly discovered protein sequences requires systematic and comprehensive analysis. such an analysis, embodied within the ds geneatlas pipeline, has been used to critically evaluate the severe acute respiratory syndrome (sars) genome with the goal of identifying new potential targets for viral therapeutic intervention. this paper discusses several new functional hypotheses on the roles played by the constituent gene product ... | 2003 | 14623076 |
| activation of ap-1 signal transduction pathway by sars coronavirus nucleocapsid protein. | in march 2003, a novel coronavirus was isolated from patients exhibiting atypical pneumonia and subsequently proven to be the causative agent of the disease now referred to as severe acute respiratory syndrome (sars). the complete genome of the sars coronavirus (sars-cov) has since been sequenced. the sars-cov nucleocapsid (sars-cov n) shares little homology with other members of the coronavirus family. to determine if the n protein is involved in the regulation of cellular signal transduction, ... | 2003 | 14623261 |
| potential for antiviral treatment of severe acute respiratory syndrome. | severe acute respiratory syndrome is a new, sometimes lethal disease of humans that is caused by a novel coronavirus. to date there have been over 750 related deaths and there is clearly an urgent need to develop specific antiviral drugs to combat this disease. in this review, the authors shall focus on the molecular biology of the coronavirus and suggest how this information can be used to identify possible targets for antiviral drugs. | 2003 | 14624107 |
| viral discovery and sequence recovery using dna microarrays. | because of the constant threat posed by emerging infectious diseases and the limitations of existing approaches used to identify new pathogens, there is a great demand for new technological methods for viral discovery. we describe herein a dna microarray-based platform for novel virus identification and characterization. central to this approach was a dna microarray designed to detect a wide range of known viruses as well as novel members of existing viral families; this microarray contained the ... | 2003 | 14624234 |
| air pollution and case fatality of sars in the people's republic of china: an ecologic study. | severe acute respiratory syndrome (sars) has claimed 349 lives with 5,327 probable cases reported in mainland china since november 2002. sars case fatality has varied across geographical areas, which might be partially explained by air pollution level. | 2003 | 14629774 |
| sars virus: the beginning of the unraveling of a new coronavirus. | severe acute respiratory syndrome (sars) virus caused a severe outbreak in several regions of the world in 2003. the virus is a novel coronavirus, which may have an origin in wild animals such as civet cats in southern china. its genome structure, gene expression pattern and protein profiles are similar to those of other coronaviruses. however, distinct patterns of several open reading frames in the sars virus genome may contribute to its severe virulence. the potential mutability of the coronav ... | 2003 | 14631105 |
| breakthrough of the year. sars: a pandemic prevented. | | 2003 | 14684791 |
| [analysis on the sars-cov genome of pumc01 isolate]. | to perform variation and phylogenetics analysis on the sars-cov genome sequence (pumc01) isolated in the peking union medical college hospital. | 2003 | 14650143 |
| [cdnas cloning of sars-cov pumc2 viral genome]. | to get the cdna clones which cover the whole genome of sars-cov pumc2 strain. | 2003 | 14650144 |
| [cloning, expression and purification of sars coronavirus pumc2 strain nucleocapsid protein]. | to clone, express and purify nucleocapsid protein from sars coronavirus pumc2 strain. | 2003 | 14650145 |
| canadian severe acute respiratory syndrome (sars) experience. | | 2003 | 14694900 |
| [isolation and identification of sars-coronavirus in nasal and throat swabs collected from clinically diagnosed sars patients]. | to isolate and identify sars-coronavirus in nasal and throat swabs collected from clinically diagnosed severe acute respiratory syndrome (sars) patients. | 2003 | 14650149 |
| [reliability of detecting sars-cov antibody for diagnosis of sars]. | to discuss the reliability of sars-cov antibody detection for sars diagnosis. | 2003 | 14650153 |
| the sars-cov s glycoprotein: expression and functional characterization. | we have cloned, expressed, and characterized the full-length and various soluble fragments of the sars-cov (tor2 isolate) s glycoprotein. cells expressing s fused with receptor-expressing cells at neutral ph suggesting that the recombinant glycoprotein is functional, its membrane fusogenic activity does not require other viral proteins, and that low ph is not required for triggering membrane fusion; fusion was not observed at low receptor concentrations. s and its soluble ectodomain, s(e), were ... | 2003 | 14651994 |
| sars--lessons from two singapore general hospital cases. | two cases of severe acute respiratory syndrome (sars) that occurred in singapore general hospital are described. the lessons learnt are outlined. | 2003 | 14700422 |
| [clinical analysis of patients with severe acute respiratory syndrome in beijing area]. | to study the epidemiological, clinical and laboratory characteristics, treatment and prognosis of patients with severe acute respiratory syndrome (sars). | 2003 | 14703443 |
| a real-time pcr for sars-coronavirus incorporating target gene pre-amplification. | an enhanced polymerase chain reaction (pcr) assay to detect the coronavirus associated with severe acute respiratory syndrome (sars-cov) was developed in which a target gene pre-amplification step preceded taqman real-time fluorescent pcr. clinical samples were collected from 120 patients diagnosed as suspected or probable sars cases and analyzed by conventional pcr followed by agarose gel electrophoresis, conventional taqman real-time pcr, and our enhanced taqman real-time pcr assays. an amplic ... | 2003 | 14652014 |
| genomic characterisation of the severe acute respiratory syndrome coronavirus of amoy gardens outbreak in hong kong. | severe acute respiratory syndrome (sars) is a global health concern. in hong kong, two major outbreaks, one hospital based and the other in the amoy gardens apartments, were identified. the frequency of diarrhoea, admission to intensive care, and mortality differed significantly between the two outbreaks. we did genomic sequencing for viral isolates from five amoy gardens patients. the virus sequence was identical in four of these five patients. the sequence data from one hospital case and the f ... | 2003 | 14654320 |
| [origin of sars from accelerated evolution of a virus by combined pollution]. | the sars virus origin which obviously differs from the theory of out-space origin and the theory of wildlife origin was theoretically discussed based on principles of pollution-evolution ecology, pathogenesis and infectant characteristics of sars, and some relevant experimental evidence. it emphasizes on the fact that increasingly combined pollution in china provides a substantial basis for virus evolution. gene recombination and fusion of more than one virus in ecosystems should take charge wit ... | 2003 | 14655379 |
| a clinicopathological study of three cases of severe acute respiratory syndrome (sars). | the severe acute respiratory syndrome (sars) caused a large outbreak of atypical pneumonia in beijing, china from early march 2003. we report the pathological features from three patients who died of sars. | 2003 | 14660106 |
| genomic sequencing of a sars coronavirus isolate that predated the metropole hotel case cluster in hong kong. | | 2004 | 14709660 |
| development of a sars vaccine: an industrial perspective on the global race against a global disease. | | 2003 | 14711331 |
| the new normal: a sars diary. | | 2003 | 14662664 |
| my experience with sars. | | 2003 | 14662665 |
| profile of antibodies to the nucleocapsid protein of the severe acute respiratory syndrome (sars)-associated coronavirus in probable sars patients. | profiles of antibodies to the nucleocapsid protein of the severe acute respiratory syndrome (sars)-associated coronavirus in 445 probable sars patients and 3,749 healthy people or non-sars patients were analyzed by antigen-capturing enzyme-linked immunosorbent assay. antinucleocapsid antibodies were elucidated in 17.5% of the probable sars patients 1 to 7 days after the onset of symptoms and in 80% of the patients 8 to 14 days after the onset. about 90% of the probable sars patients were positiv ... | 2004 | 14715574 |
| the secret life of ace2 as a receptor for the sars virus. | the membrane-associated carboxypeptidase angiotensin-converting enzyme 2 (ace2) is an essential regulator of heart function. now, li at al. identify and characterize an unexpected second function of ace2 as a partner of the sars-cov spike glycoprotein in mediating virus entry and cell fusion. | 2003 | 14675530 |
| severe acute respiratory syndrome (sars)--paradigm of an emerging viral infection. | an acute and often severe respiratory illness emerged in southern china in late 2002 and rapidly spread to different areas of the far east as well as several countries around the globe. when the outbreak of this apparently novel infectious disease termed severe acute respiratory syndrome (sars) came to an end in july 2003, it had caused over 8000 probable cases worldwide and more than 700 deaths. starting in march 2003, the world health organization (who) organised an unprecedented international ... | 2004 | 14675864 |
| [consensus of the management of severe acute respiratory syndrome]. | | 2003 | 14723215 |
| [cardiac arrest in severe acute respiratory syndrome: analysis of 15 cases]. | to investigate the causes for cardiac arrest in severe acute respiratory syndrome (sars) patients. | 2003 | 14633442 |
| [amplification and cloning of the n gene of sars-associated coronavirus]. | to amplify and clone the n gene of severe acute respiratory syndrome-associated coronavirus. | 2004 | 14724092 |
| quantitative analysis and prognostic implication of sars coronavirus rna in the plasma and serum of patients with severe acute respiratory syndrome. | the availability of an early diagnostic tool for severe acute respiratory syndrome (sars) would have major public health implications. we investigated whether the sars coronavirus (sars-cov) can be detected in serum and plasma samples during the early stages of sars and studied the potential prognostic implications of such an approach. | 2003 | 14633867 |
| assessment of immunoreactive synthetic peptides from the structural proteins of severe acute respiratory syndrome coronavirus. | the widespread threat of severe acute respiratory syndrome (sars) to human life has spawned challenges to develop fast and accurate analytical methods for its early diagnosis and to create a safe antiviral vaccine for preventive use. consequently, we thoroughly investigated the immunoreactivities with patient sera of a series of synthesized peptides from sars-coronavirus structural proteins. | 2003 | 14633869 |
| proliferative growth of sars coronavirus in vero e6 cells. | an isolate of sars coronavirus (strain 2003va2774) was obtained from a patient and used to infect vero e6 cells. the replication cycle of the virus was followed from 1 to 30 h post-infection (p.i.). it was surprising to observe the swift growth of this human virus in vero cells. within the first hour of infection, the most obvious ultrastructural change was the proliferation of the golgi complexes and related vesicles accompanied by swelling of some of the trans-golgi sacs. extracellular virus p ... | 2003 | 14645910 |
| asymptomatic severe acute respiratory syndrome-associated coronavirus infection. | | 2003 | 14725258 |
| sars 3: are we ready? | | 2003 | 14690300 |
| the virus that changed my world. | | 2003 | 14691538 |
| an indolent case of severe acute respiratory syndrome. | severe acute respiratory syndrome (sars) is a highly contagious and typically rapidly progressive form of atypical pneumonia, which spread from asia to many parts of the world in early 2003. clinical diagnosis of sars requires the presence of unremitting fever and progressive pneumonia despite antibiotic therapy, particularly in the presence of lymphopenia and raised transaminase levels. we report the case of a woman who had undergone a successful allogeneic bone marrow transplant for acute myel ... | 2004 | 14695107 |
| sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction. | a quantitative, real-time, nested polymerase chain reaction (pcr) method, combining the high sensitivity of nested pcr with time-saving real-time instrumentation, was developed for large-scale screening for severe acute coronavirus (sars) coronavirus. forty-six clinical specimens were analyzed by this method, and results were compared with those obtained by conventional, single-round, real-time reverse-transcriptase pcr (rt-pcr) performed in parallel. of the 17 positive results, 2 identified by ... | 2004 | 14699465 |
| crouching tiger, hidden dragon: the laboratory diagnosis of severe acute respiratory syndrome. | | 2004 | 14699466 |
| sars: lessons learned from other coronaviruses. | the identification of a new coronavirus as the etiological agent of severe acute respiratory syndrome (sars) has evoked much new interest in the molecular biology and pathogenesis of coronaviruses. this review summarizes present knowledge on coronavirus molecular biology and pathogenesis with particular emphasis on mouse hepatitis virus (mhv). mhv, a member of coronavirus group 2, is a natural pathogen of the mouse; mhv infection of the mouse is considered one of the best models for the study of ... | 2003 | 14733734 |
| molecular biological analysis of genotyping and phylogeny of severe acute respiratory syndrome associated coronavirus. | sars-cov is the causative agent of severe acute respiratory syndrome (sars) which has been associated with outbreaks of sars in guangdong, hong kong and beijing of china, and other regions worldwide. sars-cov from human has shown some variations but its origin is still unknown. the genotyping and phylogeny of sars-cov were analyzed and reported in this paper. | 2004 | 14733771 |
| [sars-associated coronavirus gene fragments were detected from a suspected pediatric sars patient]. | a special "fever and cough" clinic was set up at the children's hospital affiliated to capital institute of pediatrics for children with symptoms of fever and cough in late april when the severe acute respiratory syndrome (sars) epidemic was at its peak in beijing to separate the children with fever from others during their visit to the outpatient department. | 2003 | 14733796 |
| [clinical and chest x-ray characteristics of 5 cases with severe acute respiratory syndrome in children in shenzhen area]. | to explore clinical and chest x-ray features of sars in children to facilitate correct diagnosis. | 2003 | 14733797 |
| canadian researchers testing sars vaccine in china. | | 2004 | 14734423 |
| analysis of deaths during the severe acute respiratory syndrome (sars) epidemic in singapore: challenges in determining a sars diagnosis. | an outbreak of severe acute respiratory syndrome (sars), an infectious disease attributed to a novel coronavirus, occurred in singapore during the first quarter of 2003 and led to 204 patients with diagnosed illnesses and 26 deaths by may 2, 2003. twenty-one percent of these patients required admission to the medical intensive care unit. during this period, the center for forensic medicine, health sciences authority, singapore, performed a total of 14 postmortem examinations for probable and sus ... | 2004 | 14736283 |
| wet markets--a continuing source of severe acute respiratory syndrome and influenza? | context: live-animal markets (wet markets) provide a source of vertebrate and invertebrate animals for customers in tropical and subtropical regions of the world. wet markets sell live poultry, fish, reptiles, and mammals of every kind. live-poultry markets (mostly chicken, pigeon, quail, ducks, geese, and a wide range of exotic wild-caught and farm-raised fowl) are usually separated from markets selling fish or red-meat animals, but the stalls can be near each other with no physical separation. ... | 2004 | 14738798 |
| wet markets--a continuing source of severe acute respiratory syndrome and influenza? | context: live-animal markets (wet markets) provide a source of vertebrate and invertebrate animals for customers in tropical and subtropical regions of the world. wet markets sell live poultry, fish, reptiles, and mammals of every kind. live-poultry markets (mostly chicken, pigeon, quail, ducks, geese, and a wide range of exotic wild-caught and farm-raised fowl) are usually separated from markets selling fish or red-meat animals, but the stalls can be near each other with no physical separation. ... | 2004 | 14738798 |
| infectious diseases. second lab accident fuels fears about sars. | | 2004 | 14704402 |
| antigenicity and receptor-binding ability of recombinant sars coronavirus spike protein. | severe acute respiratory syndrome (sars) is an emerging infectious disease associated with a novel coronavirus and causing worldwide outbreaks. sars coronavirus (sars-cov) is an enveloped rna virus, which contains several structural proteins. among these proteins, spike (s) protein is responsible for binding to specific cellular receptors and is a major antigenic determinant, which induces neutralizing antibody. in order to analyze the antigenicity and receptor-binding ability of sars-cov s prot ... | 2004 | 14706633 |
| tissue and cellular tropism of the coronavirus associated with severe acute respiratory syndrome: an in-situ hybridization study of fatal cases. | severe acute respiratory syndrome (sars) is a new human infectious disease with significant morbidity and mortality. the disease has been shown to be associated with a new coronavirus (sars-cov). the clinical and epidemiological aspects of sars have been described. moreover, the viral genome of sars-cov has been fully sequenced. however, much of the biological behaviour of the virus is not known and data on the tissue and cellular tropism of sars-cov are limited. in this study, six fatal cases o ... | 2004 | 14743497 |
| [clinical analysis of pediatric sars cases in beijing]. | to study clinical characteristics of pediatric sars cases in beijing. | 2003 | 14744375 |