| bronchoscopy with bronchoalveolar lavage in the evaluation of pulmonary complications of bone marrow transplantation in children. | pulmonary complications are a frequent cause of morbidity and mortality following bone marrow transplantation. we examined the results of flexible bronchoscopy (fb) and bronchoalveolar lavage (bal) in 27 pediatric bone marrow transplant (bmt) recipients with 29 episodes of pulmonary complications. bone marrow transplant was performed for a variety of malignancies and hematologic disorders. median age of bmt was 10.3 years (range, 1.7-17.6 years). median time of fb following bmt was 60 days (rang ... | 1992 | 1315947 |
| [pressing problems of acute respiratory viral diseases in children]. | | 1979 | 225874 |
| activation of human eosinophils in vitro by respiratory syncytial virus. | to determine the nature of the interaction between viruses and eosinophils, normodense eosinophils were separated from the blood of healthy volunteers and incubated in vitro with respiratory syncytial virus (rsv). after incubation for 2 h with the virus, 29.5 +/- 15.8% of the eosinophils demonstrated specific binding of the virus to the cell membrane, as detected by fluorescent staining with an anti-rsv mab. superoxide production and leukotriene c4 release were measured as determinants of cell a ... | 1992 | 1324464 |
| an alternative method for evaluating potency of modified live canine parainfluenza virus vaccine. | | 1978 | 226552 |
| increased resistance to influenza as a possible source of heterozygote advantage in cystic fibrosis. | cystic fibrosis is the most common lethal or semi-lethal genetic disease in caucasians of central european origin, among whom it is inherited as an autosomal recessive trait at a frequency approximately 10 times that expected from recurrent mutation alone. a decreased sialic acid content has been observed in cell surface glycoproteins on cystic fibrosis fibroblasts and in numerous soluble glycoprotein preparations from cystic fibrosis homozygotes. sialic acid residues on cell surface glycoconjug ... | 1979 | 226848 |
| enhanced detection of respiratory viruses using the shell vial technique and monoclonal antibodies. | the shell vial technique using a549 and mdck cells, coupled with the use of bartels respiratory viral monoclonal antibodies, was evaluated initially for the detection of 28 previously isolated respiratory viruses. all viruses were recovered and correctly identified. the shell vial-monoclonal antibody technique was then evaluated for virus isolation from 338 respiratory specimens and compared with the conventional tube method. both methods gave rise to a total of 83 virus isolates. of these isola ... | 1992 | 1331151 |
| transmission of infectious diseases in children. | a rational approach to the control of pediatric infectious diseases requires an appreciation of their epidemiology and mechanisms of transmission. the setting in which the exposure occurs may have a major influence on the risk of infection. unfortunately, the epidemiology of many important pediatric pathogens is complex and incompletely understood. recent advances in diagnostic microbiology and molecular epidemiology may help to solve some of the remaining riddles. | 1992 | 1332011 |
| viral pneumonia in the immunocompromised adult with neoplastic disease: the role of common community respiratory viruses. | until recently, the viruses most often identified in lower respiratory tract infections among immunocompromised adults with neoplastic disease have been the herpesviruses (cytomegalovirus, herpes simplex virus, varicella-zoster virus, human herpesvirus-6) and adenovirus. these viral pneumonias occur with greater frequency and/or severity among these adults than among immunocompetent adults. during the last decade, mounting evidence has suggested that the rna viruses that commonly cause lower res ... | 1992 | 1332171 |
| rapid detection of respiratory viruses using mixtures of monoclonal antibodies on shell vial cultures. | eleven hundred and thirty-three clinical specimens submitted to the laboratory for diagnosis of respiratory virus infections were tested by direct immunofluorescence (dif) for respiratory syncytial virus (rsv), by shell vial culture, and by conventional cell culture. the shell vial cultures were stained with 8 different monoclonal antibodies both 1 day and 3-7 days after inoculation. in order to limit the cost and the workload, mixtures of monoclonal antibodies were used. coverslips with hep-2 c ... | 1992 | 1334129 |
| studies on the epidemiology of child infections. 3. parainfluenza viruses (types 1-4) and respiratory syncytial virus infections. | a seroepidemiological study on the rate of neutralizing antibodies for parainfluenza viruses (types 1-4) and for the respiratory syncytial virus in 2,514 infants and children between 0 and 15 years, residing in bari and its hinterland was carried out. positive results were very high for both the individual parainfluenza serotypes (81.7% for type 3, 78.0% for type 1, 76.6% for type 4, 71.9% for type 2) and for respiratory syncytial virus (88.5%). the pattern of infections due to respiratory syncy ... | 1992 | 1335108 |
| detection of multiple retroviral infections in cattle and cross-reactivity of bovine immunodeficiency-like virus and human immunodeficiency virus type 1 proteins using bovine and human sera in a western blot assay. | bovine antibovine immunodeficiency-like virus (biv) antibodies were detected by western blot analysis (wba) using a chemiluminescence protocol. bovine sera with anti-biv activity, obtained from cows in two dairy herds, had antibodies directed against a variety of biv-specific antigens indicating chronic infections. these sera were also tested for serological reactivity against bovine leukemia virus (blv) and bovine syncytial virus (bsv). cows most commonly had anti-bsv antibodies (12 of 39). evi ... | 1992 | 1335835 |
| etiologic differentiation in lower respiratory tract virus infections by indirect immunofluorescence. | | 1979 | 227647 |
| ribavirin aerosols and respiratory syncytial virus infection. | | 1992 | 1346054 |
| cd4+ t cells clear virus but augment disease in mice infected with respiratory syncytial virus. comparison with the effects of cd8+ t cells. | respiratory syncytial (rs) virus-specific t cell lines were derived from the spleens of balb/c mice primed by intranasal infection with rs virus. the lines were expanded by repeated antigenic stimulation in vitro, and separated into cd4+ and cd8+ t cell-enriched fractions by immunomagnetic adhesion. the effects of passive transfer of these fractions into rs virus infected mice were observed. the most severe immunopathological changes were seen in mice receiving cd4+ cells. transfer of cd4+, cd8+ ... | 1992 | 1351433 |
| nosocomial infection with respiratory syncytial virus. | | 1992 | 1357459 |
| prospective controlled study of four infection-control procedures to prevent nosocomial infection with respiratory syncytial virus. | to determine the most effective infection control procedure in preventing nosocomial infection with respiratory syncytial virus (rsv), we did a prospective controlled study of four infection-control strategies in four wards in a large paediatric hospital in the west of scotland. all children under two years old admitted to four general wards during three winter rsv epidemics (1989-92) were screened for rsv infection (by nasopharyngeal aspirate and direct immunofluorescence) within 18 hours of ad ... | 1992 | 1357462 |
| virus-induced apnoea and theophylline. | | 1992 | 1360064 |
| early interactions of viruses with cellular membranes. | | 1979 | 228540 |
| reshaping a human monoclonal antibody to inhibit human respiratory syncytial virus infection in vivo. | we transferred the complementarity determining regions from a murine monoclonal antibody that neutralizes infection by respiratory syncytial virus (rsv) to a human igg1 monoclonal antibody. the resulting reshaped human antibody lost affinity for rsv, but an additional alteration to one of the framework regions restored binding affinity and specificity. this second generation reshaped human monoclonal antibody cross-reacted with all clinical isolates of rsv tested and both prevented disease and c ... | 1991 | 1367535 |
| rapid detection of respiratory syncytial virus in nasopharyngeal aspirates by reverse transcription and polymerase chain reaction amplification. | a rapid method for detection of respiratory syncytial virus (rsv) in nasopharyngeal aspirates, involving a combination of reverse transcription and polymerase chain reaction amplification (rt-pcr), has been developed. the rt-pcr assay employs oligonucleotide primers specific for the region of the rsv genome which encodes the f1 subunit of the fusion (f) glycoprotein. other respiratory viruses do not give a positive reaction. the rt-pcr assay was tested on 202 nasopharyngeal aspirates collected f ... | 1992 | 1374080 |
| nosocomial respiratory syncytial virus infection in a newborn nursery. | the nosocomial spread of respiratory syncytial virus (rsv) was studied in a newborn nursery in benin city, nigeria at a time the virus was known to be highly prevalent in the community. nasopharyngeal washings were obtained from babies on admission and, thereafter, every 4 days until discharged. questionnaires were administered to medical personnel with upper respiratory tract infection (urti). rsv was detected by an elisa technique. a total of 56 babies were studied, made up of 33 preterm and 2 ... | 1992 | 1381895 |
| protective epitopes on the fusion protein of respiratory syncytial virus recognized by murine and bovine monoclonal antibodies. | the regions of the fusion protein of respiratory syncytial virus (rsv) that react with neutralizing, fusion-inhibiting and highly protective bovine and murine monoclonal antibodies (mabs) were mapped by two methods: (i) competitive binding assays and (ii) production and analysis of antibody-escape mutants. competitive binding assays with 16 murine and 10 bovine mabs identified 11 antigenic sites on the fusion (f) protein, many of which overlapped extensively, and indicated that cattle, a natural ... | 1992 | 1383403 |
| characterization of two antigenic sites recognized by neutralizing monoclonal antibodies directed against the fusion glycoprotein of human respiratory syncytial virus. | two antigenic sites recognized by neutralizing monoclonal antibodies (mabs) directed against the fusion (f) glycoprotein of human respiratory syncytial virus were mapped on the primary structure of the protein by (i) the identification of amino acid substitutions selected in antibody-escape mutants and (ii) the reactivity of synthetic peptides with mabs. the first site contained several overlapping epitopes which were located within the trypsin-resistant amino-terminal third of the large f1 subu ... | 1992 | 1383404 |
| [a serological study of respiratory infections in pilgrims to mecca]. | | 1978 | 229604 |
| an r.s.v.p. to r.s.v. (respiratory syncytial virus): declining mortality rates. | | 1992 | 1395656 |
| respiratory syncytial virus morbidity and mortality estimates in congenital heart disease patients: a recent experience. | to determine recent morbidity and mortality rates from respiratory syncytial virus infection in a pediatric congenital heart disease population. | 1992 | 1395661 |
| comparison of a new commercial enzyme immunoassay for rapid detection of respiratory syncytial virus. | two rapid methods for detection of respiratory syncytial virus in respiratory specimens were compared: direct immunofluorescence assay (dfa) with monoclonal antibody and an enzyme immunoassay (eia) (test-pack rsv). ninety-five nasopharyngeal washings and aspirates from 51 children were examined; the patients were hospitalized during a winter outbreak of rsv infection in the first trimester of 1990. a total of 41.0% and 56.8% of these samples were positive by eia and dfa respectively. considering ... | 1992 | 1396733 |
| the respiratory syncitial virus and its role in acute bronchiolitis. | | 1992 | 1396923 |
| five years of sentinel surveillance of acute respiratory infections (1985-1990): the benefits of an influenza early warning system. | for the last five years, the brussels institute of hygiene and epidemiology has been involved in the surveillance of acute respiratory infections (ari). the four indicators used (number of encounters of ari by gp's/100 encounters, virus isolations, absenteeism and mortality) are discussed. a regression procedure is applied to the data collected by a sentinel network of general practitioners (gp's). this procedure permits the baseline to be visualized and an epidemic threshold to be determined in ... | 1992 | 1397213 |
| respiratory syncytial virus puzzle: clinical features, pathophysiology, treatment, and prevention. | | 1992 | 1403380 |
| chronic cough in children. | | 1992 | 1404826 |
| respiratory syncytial virus and the use of ribavirin. | | 1992 | 1406102 |
| [the preventive effect of interferon on upper respiratory disease. 1. the influence of interferon on the serum hi antibody titers against influenza and parainfluenza viruses (author's transl)]. | | 1979 | 231626 |
| first-year infections after initial hospitalization in low birth weight infants with and without bronchopulmonary dysplasia. | symptoms of infection, specific infections, antibiotic course and hospitalizations were evaluated prospectively by using monthly questionnaires after initial hospitalization in 73 low birth weight (lbw) infants (less than 1751 g) and a subgroup of 19 infants with bronchopulmonary dysplasia (bpd) compared with 63 full-term controls matched for age, sex and home locality. the immunoglobulin g, a, m and e levels of the lbw infants were measured at corrected ages of 1, 2, 4, 6, 8, 10 and 12 months. ... | 1992 | 1411318 |
| sero-epidemiological studies of respiratory syncytial and adenoviruses in children in ibadan, nigeria, 1985-1988. | retrospective and prospective serological surveys to determine the prevalence of respiratory syncytial virus (rsv) and adenovirus (adv) infections in children with respiratory diseases were carried out from 1985 to 1988 at the university college hospital, ibadan, nigeria. 306 sera from subjects between 3 months and 12 years old were assayed by complement fixation test for antibodies to both viruses. second samples were available from 42 of the subjects for antibody conversion testing. the rate v ... | 1992 | 1412660 |
| immuno-gold labelling of turkey rhinotracheitis virus. | the morphology and morphogenesis of five viruses isolated in great britain, france and south africa from turkeys with rhinotracheitis were examined. the five isolates were antigenically related by immunofluorescence and were indistinguishable by negative contrast and thin section electron microscopy. negative contrast electron microscopy of infected vero cell cultures revealed ortho- or paramyxovirus-like particles and helical nucleocapsids 14 nm in diameter with a pitch of 6 nm. the viral natur ... | 1992 | 1414097 |
| effects of allergen challenge on plasma concentrations of prostaglandins, thromboxane b2, and histamine in calves infected with bovine respiratory syncytial virus. | to examine the influence of allergen-induced type-1 hypersensitivity on the pathogenesis of bovine respiratory syncytial virus (brsv) infection, we sensitized calves by aerosol to micropolyspora faeni (mf) and challenge exposed them during infection with brsv. the development of mf-specific ige serum concentrations was confirmed by elisa. the dynamics of arachidonic acid metabolism and histamine release during a type-1 hypersensitivity reaction in the bovine lung were studied by quantitating the ... | 1992 | 1416375 |
| field trials on a live bovine respiratory syncytial virus vaccine in calves. | field trials were carried out in calves using a live bovine respiratory syncytial (brs) virus vaccine prepared from the attenuated brs virus, strain rs-52. two hundred seventy-five and 353 calves were vaccinated intranasally and intramuscularly, respectively. no undesirable postvaccinal reactions were observed in the vaccinated calves. of the serum neutralizing (sn) antibody negative calves 89.7% (26/29) and 92.8% (90/97) developed sn antibody 1 month after intranasal and intramuscular vaccinati ... | 1992 | 1420579 |
| pathology of morbillivirus infection in striped dolphins (stenella coeruleoalba) from valencia and murcia, spain. | during the summer and fall of 1990 hundreds of striped dolphins (stenella coeruleoalba) died in the spanish mediterranean as a result of morbillivirus infection. a pathological investigation was carried out on dolphins from valencia and murcia which were among the first to die in the epizootic. the dolphins were in poor body condition and pneumonia was the main necropsy finding. microscopic lung lesions characterized by necrosis of bronchial and bronchiolar epithelium and infiltration of alveoli ... | 1992 | 1423061 |
| respiratory syncytial virus infections. | | 1992 | 1424399 |
| evaluation of three rapid enzyme immunoassays and cell culture for detection of respiratory syncytial virus. | three rapid enzyme immunoassay techniques for the detection of respiratory syncytial virus antigen (becton dickinson directigen rsv, abbott rsv testpack and abbott rsv eia) and cell culture were evaluated in a total of 250 nasal washings. the sensitivity and specificity were 62% and 76% respectively for directigen, 64% and 86% for rsv testpack, and 76% and 81% for rsv eia, taking cell culture as the reference method. agreement between cell culture and eia techniques was 79% (70 positive and 128 ... | 1992 | 1425717 |
| immunotherapy of respiratory syncytial virus infection in cotton rats (sigmodon fulviventer) using igg in a small-particle aerosol. | to determine whether aerosolized igg can be used effectively in the treatment of respiratory syncytial virus (rsv) infections, cotton rats were infected intranasally with rsv and treated 3 days later with human igg containing anti-rsv antibodies delivered in a small-particle aerosol. pulmonary histology and virus titers were determined 24 h after igg treatment. a single 15-min exposure to aerosolized igg did not exacerbate pulmonary pathology and effected a 50-fold reduction in pulmonary virus t ... | 1992 | 1431258 |
| intensive care course and outcome of patients infected with respiratory syncytial virus. | picu admissions of 97 children positive for respiratory syncytial virus on fluorescent antibody screening were reviewed; 68% of 44 patients without history of preceding disease (group i) and 79% of 53 patients with preceding pulmonary, cardiac, or other disease (group ii) required ventilation. in group i ventilated children weighted significantly less (p = 0.001) and were of lower chronological (p = 0.02) and post-conceptional ages (p = 0.02) than those not ventilated. eighteen infants ventilate ... | 1992 | 1437327 |
| the role of viruses and mycoplasma pneumoniae in lower respiratory tract infections in childhood. | acute lower respiratory tract infections are one of the major causes of childhood morbidity and mortality in developing countries. this study was undertaken at hacettepe university children's hospital to determine the role of viruses and mycoplasma pneumoniae in respiratory tract infections in children. eighty-three patients with lower respiratory tract infections were selected at random from among the children admitted to the hospital for evaluation of respiratory symptoms. acute and convalesce ... | 1992 | 1440953 |
| [clinical characteristics of respiratory-syncytial infection in adults]. | | 1977 | 604645 |
| bovine respiratory syncytial virus-specific immune responses in cattle following immunization with modified-live and inactivated vaccines. analysis of proliferation and secretion of lymphokines by leukocytes in vitro. | cattle were immunized with vaccines containing modified-live or inactivated bovine respiratory syncytial virus (brsv) and lymphocyte proliferative responses and cytokine secretion were monitored sequentially. compared to pre-inoculated values, significant increases in proliferative responses to modified-live brsv were detectable by day 7 after the primary immunization with the vaccine containing inactivated brsv, and by 7 days after the second immunization with modified-live virus. after a third ... | 1992 | 1441227 |
| bovine respiratory syncytial virus-specific immune responses in cattle following immunization with modified-live and inactivated vaccines. analysis of the specificity and activity of serum antibodies. | cattle were immunized with vaccines containing modified-live or inactivated bovine respiratory syncytial virus (brsv) and serum antibody responses were analyzed. compared with preinculation values, at day 14 after two biweekly immunizations with modified-live or inactivated vaccines there were significant increases in brsv-specific titers in the sera of cattle that received both types of vaccines, as determined by a whole cell elisa. using a blocking elisa and radioimmune precipitation it was de ... | 1992 | 1441228 |
| [yucaipa-like viruses isolated in kazakhstan in 1987-1989]. | from domestic birds 13 strains of avian paramyxoviruses, serotype 1, and 14 strains of serotype 2 were isolated. avian paramyxoviruses, serotype 2, differ antigenically and biologically from each other and from the prototype variant chicken/yucaipa/california/56. the virus was also detected experimentally in birds having contact with the infected specimens. examinations of avian blood sera revealed wide dissemination of viruses related to the chicken/yucaipa/56 strain in domestic bird breeding f ... | 1992 | 1441429 |
| [the use of a virazole aerosol in young children with a respiratory syncytial virus infection]. | the therapeutic effect of aerosol virasol was studied in young infants suffering from rs virus infection with concomitant broncho-obstructive syndrome. the study involved 60 children between 1 month and 4 years of age of whom 30 were treated with virasol and another 30 made a control group. virasol was shown to reduce the severity of the clinical picture to a considerable degree and speed up the recovery in infants with rs virus disease. virasol is recommended for inhalation treatment of infants ... | 1992 | 1441436 |
| [the characteristics of the humoral response to respiratory syncytial viral infection in adult patients with different forms of bronchitis]. | the levels of specific igg and igm were determined in 72 patients with verified respiratory syncytial (rs) virus infection: 11 patients with acute delayed bronchitis (adb), 26 with recurring bronchitis (rb) 35 with chronic obstructive bronchitis (chob), by indirect enzyme immunoassay; rs virus was detected by direct immunofluorescent technique. the activity of the inflammatory process in the bronchi was shown to depend upon rs infection activity in cases of chob and rb. rs infection was observed ... | 1992 | 1441440 |
| [biological and physico-chemical characterization of an aviary hemagglutinating agent]. | | 1978 | 645123 |
| shedding of infectious virus and virus antigen during acute infection with respiratory syncytial virus. | shedding of respiratory syncytial virus (rsv) in nasopharyngeal aspirates (npa) of hospitalized children with acute respiratory infection was studied using direct antigen detection by time-resolved fluoroimmunoassay, rapid identification of infectious virus in centrifugally inoculated cell cultures by immunoperoxidase staining and conventional virus culture. sequential npas, in which also local rsv-specific iga response was measured, were collected from children with proven rsv infection. the sh ... | 1992 | 1460457 |
| [laboratory problems of chemotherapy of viral infections in humans]. | the following problems are presented and discussed: scientific and epidemiologic basis of chemotherapy, relations of antiviral activity in vitro and in vivo especially important when new-drugs are developed and evaluated, inhibitors used in medical treatment and mechanisms of their antiviral functions, genesis and nature of virus resistance, possible role of interfering defective particles. the paper is addressed both to laboratories involved in new drugs construction as well to clinical ones pe ... | 1992 | 1470577 |
| radiographic and radionuclide lung perfusion imaging in healthy calves and calves naturally infected with bovine respiratory syncytial virus. | nine calves between three and 18 weeks old with serologically confirmed natural bovine respiratory syncytial virus infection were examined clinically, radiographically and by radionuclide lung perfusion imaging. the results were compared with those from seven healthy calves. the diseased calves were euthanased and examined pathologically, virologically and bacteriologically. the clinical signs indicated that the disease was in an acute stage. radiography of the diseased animals revealed cysts, c ... | 1992 | 1471325 |
| molecular epidemiology of respiratory syncytial virus: rapid identification of subgroup a lineages. | methods for the rapid analysis of samples of respiratory syncytial (rs) virus are described using the polymerase chain reaction (pcr) followed by restriction mapping. isolates (either clinical samples or tissue culture grown virus) can readily be divided into subgroups and then further classified into lineages. these methods enable examination of large numbers of isolates by molecular techniques, thereby facilitating research into the molecular epidemiology of the virus. | 1992 | 1474135 |
| detection of respiratory syncytial virus in acute bronchiolitis in infants. | direct detection of respiratory syncytial (rs) virus antigen in nasopharyngeal secretions (nps) provides the most rapid diagnostic test for rs infection, but more sensitive methods might be more beneficial in the study of virus shedding. rs virus rna was extracted from cells stored at -70 degrees c either in suspension with added rnase inhibitor or as a pellet without inhibitor. the rna was reverse transcribed, the resultant cdna amplified by the polymerase chain reaction and detected by ethidiu ... | 1992 | 1474378 |
| [acute infectious toxicosis as a cause of sudden infant death (clinico-anatomical parallels)]. | | 1978 | 714603 |
| [mass incidence of r.s. virus disease at the department for premature infants]. | | 1977 | 880685 |
| the molecular biology of the porcine paramyxovirus lpmv. | protein and genomic studies of a previously uncharacterized porcine paramyxovirus (designated lpmv) confirmed that it was a member of the paramyxovirus genus. the nucleotide sequences and deduced amino acids of the complete p-gene, m-gene, f-gene and hn-gene as well as the intergenic sequences have been determined. comparative sequence analysis of the m-gene of lpmv revealed the closest relationship of lpmv was to human mumps virus with a homology of 46% and 55% at the amino acid and nucleotide ... | 1992 | 1481359 |
| round table on morbilliviruses in marine mammals. | since 1988 morbilliviruses have been increasingly recognized and held responsible for mass mortality amongst harbour seals (phoca vitulina) and other seal species. virus isolations and characterization proved that morbilliviruses from seals in northwest europe were genetically distinct from other known members of this group including canine distemper virus (cdv), rinderpest virus, peste des petits ruminants virus and measles virus. an epidemic in baikal seals in 1987 was apparently caused by a m ... | 1992 | 1481361 |
| inclusion body myositis. a "slow-virus" infection of skeletal musculature? | we report the case of a 56-years old patient with clinical symptoms of an unresolved neuromuscular disease. the light microscopic studies of a muscle biopsy from the m. triceps shows the picture of a diffuse muscular atrophy. by electron microscopy, myelin-like degeneration zones with tubular-filamentous inclusions can be shown in the cytoplasma of the atrophic muscle cells. these filamentous structures correspond morphologically to the nucleocapside of paramyxoviruses. these results lead, even ... | 1977 | 926717 |
| comparison of three immunoassays for the rapid detection of bovine respiratory syncytial virus. | three enzyme-linked immunosorbent assays (eia) designed for the detection of human respiratory syncytial virus (rsv) were evaluated for the detection of bovine respiratory syncytial virus (brsv) in bovine lungs and the results were compared with those obtained by a direct fluorescent antibody assay (dfa). the eia tests used were directigen eia, kallestad pathfinder eia, and abbott rsv eia. homogenates of lung tissues obtained from 64 cattle that had died of respiratory disease were used; 32 were ... | 1992 | 1501586 |
| antibody response of calves to immunoaffinity-purified bovine respiratory syncytial virus vp70 after vaccination and challenge exposure. | immunoaffinity-purified bovine respiratory syncytial virus (brsv) fusion (f) protein elicited anti-brsv-specific antibody responses in brsv-seronegative calves. after primary vaccination, all calves seroconverted to brsv as determined by the virus neutralization (vn) test and developed anti-f protein antibodies detectable by protein immunoblot analyses. subsequent vaccinations induced greater than twofold increase in vn titer in 3 of 9 (33%) calves, and 1 calf became vn-negative, but still had n ... | 1992 | 1510306 |
| evidence of immunosuppression by bovine respiratory syncytial virus. | respiratory syncytial virus (rsv) is a major respiratory pathogen in human infants and calves. calves and lambs infected with bovine rsv show mild clinical signs but they are more susceptible to secondary infection with pasteurella haemolytica. lambs infected with p. haemolytica 6 days after experimental infection with bovine rsv had significantly greater magnitudes of fever, higher disease and lesion scores and higher mortality rates than those infected with p. haemolytica or bovine rsv alone ( ... | 1992 | 1514055 |
| improved outcome of respiratory syncytial virus infection in a high-risk hospitalized population of canadian children. pediatric investigators collaborative network on infections in canada. | to determine the outcomes in children at high risk for death or complications from respiratory disease who are hospitalized with respiratory syncytial virus (rsv) infection. | 1992 | 1517907 |
| lack of effect of respiratory syncytial virus infection on theophylline disposition in children. | conflicting reports raise a question about decreased plasma clearance (clp) of theophylline in man during viral infections. thus a dilemma exists concerning requisite dose adjustments. we examined this issue by retrospectively evaluating theophylline clp in children infected with respiratory syncytial virus (rsv). two pharmacokinetic approaches were applied to a one-compartment open model to fit theophylline concentrations during 83 hospitalizations of 76 children, 6 to 48 months of age, who rec ... | 1992 | 1517927 |
| the limited role of the human interferon system response to respiratory syncytial virus challenge: analysis and comparison to influenza virus challenge. | the respiratory syncytial virus (rsv)-induced production of interferon (ifn) by human macrophages and mononuclear leukocytes (mnl) and the sensitivities of rsv to subtypes of ifn-alpha were examined and compared to ifn production induced by influenza virus. influenza virus induced high titers of total ifn bioactivity, transcription of the ifn-alpha 1 and ifn-beta gene products and production of ifn-gamma. in contrast, rsv induced minimal or no detectable total ifn activity, and the absence of if ... | 1992 | 1522795 |
| respiratory syncytial virus: a comparison of diagnostic modalities. | this study compared prospectively viral culture for respiratory syncytial virus (rsv) with three rapid rsv antigen detection tests: rsv eia and testpack rsv (tp), and directigen rsv (dir). additionally two methods of specimen collection were compared: nasopharyngeal rub (rub) and nasopharyngeal wash (wash). true positives were defined as positive rsv viral culture or at least two positive antigen tests. one hundred ninety-eight wash specimens obtained from children 3 years of age or younger duri ... | 1992 | 1523070 |
| immunogenicity of recombinant adenovirus-respiratory syncytial virus vaccines with adenovirus types 4, 5, and 7 vectors in dogs and a chimpanzee. | recombinant adenovirus type 4, 5, and 7 expressing the fusion glycoprotein (f) gene, the attachment glycoprotein (g) gene, or both f and g genes of respiratory syncytial virus (rsv) was constructed. intratracheal immunization of dogs with ad7f induced moderate titers of rsv-neutralizing antibodies. after booster immunization with ad4f, the dogs developed high titers of rsv-specific antibody. subsequently, three two-dose vaccination regimens, ad4f/ad5f, ad7g/ad4g, and ad7fg/ad4fg, were compared w ... | 1992 | 1527411 |
| [severe respiratory syncytial virus pneumonia following bone marrow autograft. 3 cases]. | three patients developed severe respiratory syncytial virus pneumonia after bone marrow autograft for acute leukaemia. clinically, the disease presents as interstitial or bilateral alveolo-interstitial pneumonia with hypoxaemia. signs of ent infection (otitis media, sinusitis) are present in 30 percent of the cases. in all 3 patients, the syncytial virus was isolated by direct immunofluorescence in bronchoalveolar lavage fluid. in 2 patients the infection began soon after the autograft, in deepl ... | 1992 | 1532059 |
| protective activity of a human respiratory syncytial virus immune globulin prepared from donors screened by microneutralization assay. | to explore the feasibility of preparing a human immune globulin specific for respiratory syncytial virus (rsv) by screening plasma donors, the ability of seven rsv antibody assays to identify plasma-yielding igg with high virus-neutralizing and animal-protective activities was compared. igg prepared from plasma units selected by microneutralization assay had significantly higher activity in protecting mice from respiratory rsv challenge than did iggs prepared from plasmas selected by three direc ... | 1992 | 1538152 |
| putative role of platelet-activating factor in the mechanism of virus-induced asthma. | | 1992 | 1538167 |
| comparison of the ability of formalin-inactivated respiratory syncytial virus, immunopurified f, g and n proteins and cell lysate to enhance pulmonary changes in balb/c mice. | formalin-inactivated respiratory syncytial virus (fi-rsv), a lysate of hep-2 cells and proteins f, g and n, immunopurified from infected cell cultures, were compared for their ability to prevent infection and to enhance changes in lung cytology associated with rsv challenge. mice were immunized at three weekly intervals with serial dilutions of the preparations and challenged by the nasal route 1 week after the last injection; their lungs were analysed 4 days later. the concentration of the immu ... | 1992 | 1539464 |
| respiratory syncytial virus pneumonia in a lung transplant recipient: case report. | a 29-year-old man underwent bilateral lung transplantation and received maintenance immunosuppressive therapy. he was readmitted 11 months later with symptoms of cough, sneezing, and rhinorrhea. the physical examination was normal. laboratory results were significant for a reduction of fev1 and an interstitial infiltrate on chest films. the patient had recently undergone bronchoscopy for rejection surveillance, and 2 days before admission the bronchoalveolar lavage cultures returned positive for ... | 1992 | 1540615 |
| epidemic of bronchiolitis in infants. | | 1992 | 1547398 |
| class-specific antibodies to bovine respiratory syncytial virus in experimentally infected lambs. | enzyme-linked immunoabsorbent assay (elisa) was used to titrate virus-specific igg, igm and iga levels in nasal secretions, lung lavage fluids and serum samples sequentially obtained from lambs experimentally infected with bovine respiratory syncytial virus (rsv). virus-specific igg and igm responses were measured by the indirect double antibody sandwich elisa using anti-bovine rsv monoclonal antibody, as capture antibody, and peroxidase-conjugated anti-sheep igg and anti-sheep igm. virus-specif ... | 1992 | 1547834 |
| [hemolytic-uremic syndrome in children with acute viral respiratory infections]. | | 1976 | 980621 |
| reinfection of lambs with bovine respiratory syncytial virus. | eight lambs which were experimentally infected with bovine respiratory syncytial virus (rsv) when they were six to eight weeks old were challenged with the same virus seven months later. after reinfection, lambs developed mild clinical disease and the virus was isolated from nasal swabs from three lambs and peripheral blood from two lambs. reinfection resulted in changes in peripheral blood cell populations. there was an early increase in the number of cd8+ t lymphocytes and b (lca p220+) lympho ... | 1992 | 1553439 |
| respiratory syncytial virus infection of alveolar macrophages in adult transplant patients. | pulmonary epithelial cells are thought to be the primary cellular targets for infection by respiratory syncytial virus (rsv) in vivo. to determine whether other pulmonary cells are infected by rsv, bronchoalveolar lavage cells from six adult transplant patients, four of whom had acute rsv infection, were examined by in situ immunohistochemistry to identify infected lung cells. both alveolar macrophages and epithelial cells were infected with rsv in vivo. at the single-cell level, three-color imm ... | 1992 | 1554223 |
| [characteristics of clinical course of respiratory syncytial infection in young children]. | | 1976 | 995567 |
| [fatal endemic infection in a serpentarium. diagnosis, treatment and preventive measures]. | | 1976 | 1006665 |
| the efficacy of nebulized metaproterenol in wheezing infants and young children. | the benefit of beta-adrenergic agonists in the treatment of acutely wheezing infants and young children has not been well documented in the outpatient setting. to determine the efficacy of nebulized metaproterenol sulfate, 74 children aged 36 months or younger with acute wheezing participated in a double-masked, randomized, placebo-controlled clinical trial. children received nebulized metaproterenol, either as an initial treatment or after a control treatment with normal saline solution. at bas ... | 1992 | 1558072 |
| epidemic of bronchiolitis in infants. | | 1992 | 1559099 |
| mucosal t cell distribution during infection with respiratory syncytial virus. | groups of 12-week-old balb/c mice were inoculated intranasally with respiratory syncytial virus (rsv) and sacrificed at regular intervals after infection. t lymphocyte subset distribution was determined in lung tissue, bronchoalveolar lavage (bal), peripheral blood, and spleen by means of flow cytometry employing monoclonal antibodies against the t cell membrane antigens thy1.2 (pan-t), ly2 (cd8), and l3t4 (cd4). thy1.2+ cells increased in the lung from 35.4% of total lymphocytes before infectio ... | 1992 | 1564449 |
| respiratory syncytial virus: a pediatric nursing plan of care. | thirty-three pediatric cases of respiratory syncytial viral (rsv) infections were treated at providence hospital's pediatric intensive care unit (picu), anchorage, ak, between september 1987 and march 1988. our 8-bed picu nursing and respiratory therapy staff learned effective techniques in caring for patients with rsv. our aim in this article is to share our approach in providing comprehensive care for patients with rsv infections. | 1992 | 1569520 |
| lower respiratory tract infection in hospitalized children due to respiratory syncytial (rs) virus during a suspected epidemic period of rs virus in delhi. | a study of 131 children below 5 years of age seeking hospitalization due to lower respiratory tract infections (lrti), comprising 56 cases of bronchiolitis, 61 cases of bronchopneumonia, and 14 cases of other lower respiratory tract diseases showed significant occurrence of bronchiolitis in younger age groups (chi 2 = 79.21; p less than 0.001). employing two rapid techniques, viz. immunofluorescent antibody technique (ifat) and enzyme immunoassay (eia) along with tissue culture, respiratory sync ... | 1992 | 1569638 |
| respiratory syncytial virus infection reinforces reflex apnea in young lambs. | to evaluate the effect of respiratory syncytial virus (rsv) infection on reflex apnea elicited by application of water on the laryngeal mucosa, 11 healthy, term lambs were chronically instrumented at 2 wk of age. six lambs were inoculated with bovine rsv, and five lambs were mock-infected. the lambs were studied awake and unsedated before and 4, 8, 14, and 21 d after infection. rsv infection was associated with slight rhinorrhea and with moderately increased tracheal mucous discharge. there was ... | 1992 | 1570205 |
| severe respiratory syncytial virus pneumonia after autologous bone marrow transplantation: a report of three cases and review. | three patients with acute leukemia who underwent autologous bone marrow transplantation (bmt) in complete remission, developed a severe respiratory syncytial virus (rsv) pneumonia, which was fatal in two. identification of rsv was made on the products of bronchoalveolar lavage by direct immunofluorescence. as already described by others, the initial course of rsv infection varies, depending on whether it occurs sooner or later after bmt with a better prognosis in the latter situation. treatment ... | 1992 | 1571714 |
| distinguishing between respiratory syncytial virus subgroups by protein profile analysis. | we subgrouped 75 strains of respiratory syncytial virus by a protein profile method (ppm) which relies on different mobilities of the phosphoprotein in one-dimensional polyacrylamide gel electrophoresis and does not require monoclonal antibodies. when compared with enzyme immunoassay, ppm correctly subgrouped 54 of 56 subgroup a and all 19 subgroup b strains. | 1992 | 1572961 |
| humoral immunity to respiratory syncytial virus infection in the elderly. | the relationship between serum immunoglobulins and the severity and risk of respiratory syncytial virus (rsv) infection in the institutionalized elderly was prospectively assessed during the winter of 1989-1990 at a 591 bed nursing home. forty rsv infections were identified out of 149 respiratory illnesses by isolation of the virus or by a greater than or equal to 4-fold rise in rsv-specific igg by eia. acute serum rsv igg levels were similar in those with rsv infection and those with non-rsv il ... | 1992 | 1573388 |
| an outbreak of respiratory syncytial virus in a bone marrow transplant center. | an outbreak of respiratory syncytial virus (rsv) infection occurred among 31 patients in a marrow transplant center over a 13-week period beginning in january 1990. rsv infection was also documented in 35 family members and employees. of 18 patients with pneumonia, 14 (78%) died. none of 13 with upper respiratory infection died. preengraftment patients tended to develop pneumonia more frequently than did engrafted patients. early administration of ribavirin may have had a beneficial effect in pa ... | 1992 | 1583345 |
| vaccination against acute respiratory virus infections and measles in man. | several viruses may cause more or less severe acute respiratory infections in man, some of which are followed by systemic infection. only for influenza and measles are licensed vaccines available at present. the protection induced by influenza vaccines, which are based on inactivated whole virus or viral subunits, depends largely on the matching of vaccine strain and circulating virus. measles vaccines, which are based on attenuated live virus, have been quite effective in controlling the diseas ... | 1992 | 1587542 |
| epidemiology of acute lower respiratory tract infections, especially those due to haemophilus influenzae type b, in the gambia, west africa. | mortality surveys undertaken in rural areas of the gambia, a small country on the west coast of africa, indicate that acute lower respiratory tract infections (alri) are the most frequent cause of death among children and that approximately 1 in 25 rural gambian children dies from an alri before the age of 5 years. community surveys suggest that each child experiences an average of one episode of alri accompanied by radiographic changes before reaching this age. etiologic studies have shown that ... | 1992 | 1588169 |
| pulmonary eosinophilic response to respiratory syncytial virus infection in mice sensitized to the major surface glycoprotein g. | to investigate the contribution of immunity to individual respiratory syncytial (rs) virus proteins to the augmentation of pulmonary pathology, mice were scarified with recombinant vaccinia viruses (rvv) expressing individual rs virus proteins. the pulmonary response to infection with rs virus was monitored by bronchoalveolar lavage (bal). in mice vaccinated with the major surface glycoprotein (g), 14-25% of bal cells were eosinophils; these comprised less than 3% of bal cells from other groups ... | 1992 | 1591217 |
| effect of respiratory syncytial virus on adherence, colonization and immunity of non-typable haemophilus influenzae: implications for otitis media. | adherence of non-typable haemophilus influenzae to respiratory epithelium was evaluated in a cotton rat model of respiratory syncytial virus (rsv) infection. colonization with non-typable h. influenzae increased to a maximum within 4 days of rsv infection compared to rsv negative controls (4.58 +/- 0.17 vs 3.82 +/- 0.23 log colony forming units (cfu) per ml, p less than 0.05) and then declined over the subsequent 10 days (2.0 +/- 0 vs 3.78 +/- 0.39 cfu per ml, p less than 0.0001). in a second se ... | 1992 | 1592548 |
| effect of "in vitro" exposure of bovine alveolar macrophages to different strains of bovine respiratory syncytial virus. | a vaccine strain of respiratory syncytial (rs) virus and an isolate from pneumonic calves (ac2) were inoculated onto cultures of bovine alveolar macrophages recovered by lung lavage, and the functional properties of the cells observed over a period of 10 days. in most cultures no infectious virus was produced although immunofluorescence indicated the presence of virus antigens in some cells. no significant difference was noted between infected and control macrophage cultures in their capacity to ... | 1992 | 1595187 |
| pneumonia in childhood: etiology and response to antimicrobial therapy. | a prospective eight-month study was carried out in 50 children admitted to hospital for radiologically confirmed community-acquired pneumonia. a potential causative agent of infection was identified in 44 (88%) cases. using virus isolation, virus antigen detection and enzyme immunoassay serology, respiratory virus infection was diagnosed in 30 (60%) patients. antibody assays for seven bacteria and antigen detection from serum and urine for streptococcus pneumoniae produced evidence of bacterial ... | 1992 | 1597197 |
| approaches to immunization against respiratory syncytial virus. | | 1992 | 1600380 |
| successful therapy with ribavirin of late onset respiratory syncytial virus pneumonitis complicating allogeneic bone transplantation. | a 21-year-old patient developed interstitial pneumonitis nine months post bone marrow transplant for acute myeloblastic leukaemia. immunofluorescence of broncheoalveolar lavage fluid revealed the presence of respiratory syncytial virus (rsv). aerosolized ribavarin therapy resulted in rapid resolution of the pneumonitis with full recovery without any side effects. ribavarin therapy should be considered early in the management of bmt patients who develop rsv pneumonitis. | 1992 | 1600691 |
| respiratory syncytial virus infection in an adult with wegener's granulomatosis. | respiratory syncytial virus (rsv) has been documented as a pathogen in adults who are immunocompromised because of various underlying conditions. to our knowledge, this is the first report of a patient with wegener's granulomatosis (wg) and rsv infection resulting in a fatal outcome. | 1992 | 1600799 |