| mathematical modeling of the development of antioxidant activity in soybeans fermented with aspergillus oryzae and aspergillus awamori in the solid state. | the kinetics of the development fungal growth, beta-glucosidase activity, total phenolics, and dpph scavenging in soybeans fermented with aspergillus oryzae and aspergillus awamori were studied over an 8-day incubation period. modified logistic equations were then used to describe the experimental fermentation profiles. in all cases the models were consistent and the parametric estimations were statistically significant. the predicted values have high coefficients of linear correlation with the ... | 2009 | 19099459 |
| characteristics of glucose uptake by glucose- and nh4-limited grown penicillium ochrochloron at low, medium and high glucose concentration. | glucose uptake by penicillium ochrochloron (formerly penicillium simplicissimum) was studied from 0.01 to 400 mm glucose using chemostat culture and bioreactor batch culture. the characteristics of glucose uptake varied considerably with the conditions of growth, harvest and uptake assay. glucose-limited grown mycelium showed one saturable transport system [k(s) below 0.01 mm; v(max) 1.1-1.2 mmol (g dry weight)(-1)h(-1)] plus a first order process (permeability p=1.2x10(-7)cm s(-1)). ammonium-li ... | 2008 | 18722543 |
| biochemical studies on native and cross-linked aggregates of aspergillus awamori feruloyl esterase. | thermal stabilities of a native freeze dried aspergillus awamori feruloyl esterase (fae-ii) enzyme and a cross-linked feruloyl esterase aggregate (cleas) at 25-85 degrees c were evaluated and discussed. effects of some metal ions and some chemicals on the activity of both native freeze dried fae-ii enzyme and cleas were examined and explained. differential scanning calorimetry, thermogravimetry, and derived thermogravimetry, were used to observe and explain the thermal denaturation processes. st ... | 2009 | 19150457 |
| enhanced in vitro and in vivo antioxidant activity and mobilization of free phenolic compounds of soybean flour fermented with different beta-glucosidase-producing fungi. | to evaluate the soybean polyphenol glucosides bioconversion to aglycone forms by different beta-glucosidases-producing filamentous fungi to enhance their antioxidant activity. | 2009 | 19200313 |
| enrichment of phenolics and free radical scavenging property of wheat koji prepared with two filamentous fungi. | in the present study, total phenolic content and antioxidant property of wheat (54% ethanolic extract) was drastically enhanced when fermented with two gras filamentous fungi, aspergillus oryzae and aspergillus awamori nakazawa. a. oryzae yielded significant (p<0.05) amounts of phenolic compounds, dpph* (2,2-diphenyl-1-picrylhydrazyl) and abts*(+) [2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)] scavenging properties on 4th day of incubation compared to a. awamori on 5th day of incubatio ... | 2009 | 19232489 |
| purification and characteristics of feruloyl esterase from aspergillus awamori g-2 strain. | for food industry production processes and other uses, a mold that produces high levels of feruloyl esterase was obtained from laboratory mold collections and other sources. it was aspergillus awanmori g-2 that produces high levels of feruloyl esterase. the feruloyl esterase was purified using ion-exchange chromatography, size-exclusion chromatography, and hplc chromatography. the enzyme was identified as a monomer protein using size-exclusion chromatography. its optimum temperature and ph were, ... | 2008 | 19241534 |
| biodegradation of phenol by immobilized aspergillus awamori nrrl 3112 on modified polyacrylonitrile membrane. | covalent immobilization of aspergillus awamori nrrl 3112 was conducted onto modified polyacrylonitrile membrane with glutaraldehyde as a coupling agent. the polymer carrier was preliminarily modified in an aqueous solution of naoh and 1,2-diaminoethane. the content of amino groups was determined to be 0.58 mgeq g(-1). two ways of immobilization were used-in the presence of 0.2 g l(-1) phenol and without phenol. the capability of two immobilized system to degrade phenol (concentration-0.5 g l(-1) ... | 2009 | 19340590 |
| cereal-based biorefinery development: utilisation of wheat milling by-products for the production of succinic acid. | a novel wheat-based bioprocess for the production of a nutrient-complete feedstock for the fermentative succinic acid production by actinobacillus succinogenes has been developed. wheat was fractionated into bran, middlings and flour. the bran fraction, which would normally be a waste product of the wheat milling industry, was used as the sole medium in two solid-state fermentations (ssf) of aspergillus awamori and aspergillus oryzae that produce enzyme complexes rich in amylolytic and proteolyt ... | 2009 | 19539669 |
| xylan decomposition by aspergillus clavatus endo-xylanase. | agricultural and forest waste products are abundant and low-cost biomass sources useful in renewable fuel energy and feedstock preparation. hydrolysis of a major biomass component, hemicellulose, is accomplished by the action of endo-xylanases. reaction products vary in composition and degree of polymerization as a function of both feedstock and the enzyme activities utilized, ranging from monomeric sugars to complex branched polysaccharides. the study herein describes heterologous expression in ... | 2009 | 19560539 |
| expression of a fungal glucoamylase in transgenic rice seeds. | glucoamylase, which catalyses the hydrolysis of the alpha-1,4 glycosidic bonds of starch, is an important industrial enzyme used in starch enzymatic saccharification. in this study, a glucoamylase gene from aspergillus awamori, under the control of the promoter of seed storage protein gt1, was introduced into rice by agrobacterium-mediated transformation. significant glucoamylase activity was detected specifically in the seeds but not other tissues of the transgenic rice lines. the highest enzym ... | 2008 | 18588984 |
| ethanol production from starch by a coimmobilized mixed culture system of aspergillus awamori and zymomonas mobilis. | the production of ethanol from starch by a coimmobilized mixed culture system of aerobic and anaerobic microorganisms in ca-alginate gel beads was investigated. the mold aspergillus awamori was used as an aerobic amylolytic microorganism and an anaerobic bacterium, zymomonas mobilis, as an ethanol producer. by controlling the mixing ratio of the microorganisms in the inoculum size, a desirable coimmobilized mixed culture system, in which the aerobic mycelia grew on and near the oxygen-rich surfa ... | 1986 | 18555292 |
| effect of heat treatment on total phenolic and anthocyanin contents as well as antioxidant activity of the extract from aspergillus awamori-fermented black soybeans, a healthy food ingredient. | aspergillus awamori-fermented black soybeans, a healthy food ingredient, were subjected to heating at 40-100 degrees c for 30 min. after heat treatment, the methanol extract of the fermented black soybeans was examined for antioxidant activity including the 2,2-diphenyl-1-picryl-hydrozyl radical-scavenging effect and the fe(2+)-chelating ability. it was found that antioxidant activity exerted by the methanol extract was stable with heating at a temperature up to 80 degrees c for 30 min. the extr ... | 2009 | 19817642 |
| effect of additives on the activity of tannase from aspergillus awamori mtcc9299. | tannase from aspergillus awamori mtcc 9299 was purified using ammonium sulfate precipitation followed by ion-exchange chromatography. a purification fold of 19.5 with 13.5% yield was obtained. temperature of 30 degrees c and ph of 5.5 were found optimum for tannase activity. the effects of metals and organic solvents on the activity of tannase were also studied. metal ions mg(+2), mn(+2), ca(+2), na(+), and k(+) stimulated the tannase activity, while cu(+2), fe(+3), and co(+2) acted as inhibitor ... | 2010 | 19844665 |
| new oxidized sterols from aspergillus awamori and the endo-boat conformation adopted by the cyclohexene oxide system. | two new oxidized sterols 1 and 2 were obtained from the active fraction of a mangrove fungus aspergillus awamori isolated from the soils around the mangrove plant acrostichum speciosum. their structures were elucidated using spectroscopic methods as 22e-7alpha-methoxy-5alpha,6alpha-epoxyergosta-8(14),22-dien-3beta-ol (1) and 22e-3beta-hydroxy-5alpha,6alpha,8alpha,14alpha-diepoxyergosta-22-en-7-one (2). the nmr data and complete assignments for both dmso-d(6) and cdcl(3) were given. their cytotox ... | 2010 | 19877128 |
| endo- and exo-inulinases: enzyme-substrate interaction and rational immobilization. | three-dimensional models of exoinulinase from bacillus stearothermophilus and endoinulinase from aspergillus niger were built up by means of homology modeling. the crystal structure of exoinulinase from aspergillus awamori was used as a template, which is the sole structure of inulinase resolved so far. docking and molecular dynamics simulations were performed to investigate the differences between the two inulinases in terms of substrate selectivity. the analysis of the structural differences b ... | 2010 | 19941325 |
| evaluation of the number of ionogenic groups of inulinase by acid-base titration. | acid base titration showed that aspergillus awamori inulinase includes 178 asparaginic and glutamic acid residues, 20 histidine, 10 serine, and 34 lysine and tyrosine residues. denaturation temperature for this enzyme was calculated using analysis of the proportion of stabilizing and destabilizing amino acids in the molecule. | 2008 | 19110573 |
| purification and characterization of a low molecular weight endo-xylanase from mushroom termitomyces clypeatus. | a low molecular weight endo-xylanase (ec 3.2.1.8) was purified from an edible mushroom termitomyces clypeatus grown in submerged medium with oat spelt xylan. xylanase was purified to apparent homogeneity by ammonium sulfate fractionation and gel filtration chromatography. its molecular weight was determined by gel filtration chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be 12 kda. the enzyme was found to be most active at 50 degrees c and ph 5.0, being most stab ... | 2010 | 19728166 |
| characterization of a chimeric enzyme comprising feruloyl esterase and family 42 carbohydrate-binding module. | we engineered a chimeric enzyme (awfaea-cbm42) comprising of type-a feruloyl esterase from aspergillus awamori (awfaea) and family 42 carbohydrate-binding module (akcbm42) from glycoside hydrolase family 54 alpha-l-arabinofuranosidase of aspergillus kawachii. the chimeric enzyme was successfully produced in pichia pastoris and accumulated in the culture broth. the purified chimeric enzyme had an apparent relative molecular mass (m(r)) of 53,000. the chimeric enzyme binds to arabinoxylan; this in ... | 2010 | 19756576 |
| expression of feruloyl esterase from aspergillus awamori in escherichia coli: characterization and crystal studies of the recombinant enzyme. | this paper describes cloning, expression, characterization and crystallization of feruloyl esterase from aspergillus awamori. the gene coding for feruloyl esterase from a. awamori was amplified, cloned, and expressed in escherichia coli. the recombinant enzyme was purified, characterized and used for crystallization. its x-ray crystallographic data were collected and explained using molecular replacement theory. also, the tertiary structure of the recombinant enzyme-ferulic acid complex was also ... | 2010 | 20138908 |
| study of conformational changes in glucoamylase of aspergillus awamori nakazawa in presence of denaturants through cd-spectroscopy. | in present study, changes in the pattern or motifs of secondary structures of glucoamylase from aspergillus awamori nakazawa in the presence of denaturants such as urea, guanidine-hcl (gdn-hcl), and at different ph, were studied through cd-spectroscopy. it was observed that in native state glucoamylase entirely comprised of beta-sheets. cd-spectra of glucoamylase in the presence of 6 m urea lost its spectral regular characteristic; and ellipticity became zero between 207 and 218 nm. with 0.5 m u ... | 2010 | 20452763 |
| purification and characterization studies of a thermostable β-xylanase from aspergillus awamori. | this study presents data on the production, purification, and properties of a thermostable β-xylanase produced by an aspergillus awamori 2b.361 u2/1 submerged culture using wheat bran as carbon source. fractionation of the culture filtrate by membrane ultrafiltration followed by sephacryl s-200 and q-sepharose chromatography allowed for the isolation of a homogeneous xylanase (pxii-1), which was 32.87 kda according to ms analysis. the enzyme-specific activity towards soluble oat spelt xylan, whi ... | 2010 | 20549295 |
| recombinant human lactoferrin: a valuable protein for pharmaceutical products and functional foods. | lactoferrin, the main iron-binding protein of milk, has biological activities that are essential for the newborn and are beneficial for adults. given this beneficial effect, there is broad interest in exogenous sources of lactoferrin in human nutrition. consequently, several transgenic approaches to produce lactoferrin have been achieved. however, the activity of heterologous lactoferrin cannot be assumed to identically mimic that of the homologous protein. human lactoferrin obtained from yeast, ... | 2010 | 20624450 |
| sugarcane bagasse enzymatic hydrolysis: rheological data as criteria for impeller selection. | the aim of this work was to select an efficient impeller to be used in a stirred reactor for the enzymatic hydrolysis of sugar cane bagasse. all experiments utilized 100 g (dry weight)/l of steam-pretreated bagasse, which is utilized in brazil for cattle feed. the process was studied with respect to the rheological behavior of the biomass hydrolysate and the enzymatic conversion of the bagasse polysaccharides. these parameters were applied to model the power required for an impeller to operate a ... | 2010 | 20844924 |
| construction of a direct starch-fermenting industrial strain of saccharomyces cerevisiae producing glucoamylase, alpha-amylase and debranching enzyme. | to develop a strain of saccharomyces cerevisiae that produces ethanol directly from starch, two integrative vectors were constructed to allow the simultaneous multiple integration of the aspergillus awamori glucoamylase gene (ga1) and the debaryomyces occidentalis alpha-amylase gene (amy) and glucoamylase with debranching activity gene (gam1) into the chromosomes of an industrial strain of s. cerevisiae. the ga1 and amy genes were constitutively expressed under the adc1 promoter in s. cerevisiae ... | 2010 | 20131079 |
| economic analysis of the production of amylases and other hydrolases by aspergillus awamori in solid-state fermentation of babassu cake. | amylases are one of the most important industrial enzymes produced worldwide, with their major application being in ethanol manufacturing. this work investigated the production of amylases by solid-state fermentation of babassu cake, using the filamentous fungus aspergillus awamori ioc-3914. lab-scale experiments were carried out to generate input data for simulations of an industrial plant for amylase production. additionally to the target enzymes, other hydrolases (cellulases, xylanases, and p ... | 2010 | 21048867 |
| [antibacterial activity of secondary metabolites from aspergillus awamori f12 isolated from rhizospheric soil of rhizophora stylosa griff]. | to identify aspergillus awamori strain f12 isolated from rhizospheric soil of rhizophora stylosa griff and to characterize antibacterial compounds from the ethyl acetate extracts of its fermentation broth. | 2010 | 21141475 |
| synthesis of sugar fatty acid esters using a ß-xylosidase from aspergillus awamori for transxylosylation. | aspergillus awamori k4 ß-xylosidase has broad acceptor specificity. it has been used to synthesize a sugar fatty acid ester via its transxylosylation activity. one xylosyl residue was initially transferred to hexamethylene glycol as a linker with a yield of 0.36 g/g xylobiose. linoleic acid was subsequently linked to one terminal hydroxyl side of the transfer product hydroxyhexyl xyloside through an esterification reaction catalyzed by a lipase. the synthesis of hexyl linoleoyl xyloside was conf ... | 2011 | 21805184 |
| acidophilic tannase from marine aspergillus awamori btmfw032. | aspergillus awamori btmfw032, isolated from sea water, produced tannase as extracellular enzyme under submerged culture conditions. enzyme with a specific activity of 2761.89 iu/mg protein, a final yield of 0.51 %, and a purification fold of 6.32 was obtained after purification to homogeneity by ultrafiltration and gel filtration. sds-page analyses under non- reducing and reducing conditions yielded a single band of 230 kda and 37.8 kda, respectively, indicating presence of six identical monomer ... | 2010 | 21030825 |
| [the effect of point amino acid substitutions in an internal alpha-helix on thermostability of aspergillus awamori x100 glucoamylase]. | conformational flexibility of alpha-helices in glucoamylase of the fungus aspergillus awamori was studied by molecular dynamics methods. several amino acid substitutions (g127a, p128a, i136l, g137a, and g139a) optimizing intrinsic interactions in one of the alpha-helices (d) within the hydrophobic core of this protein were constructed and studied. it was found that these point mutations had different effects on the glucoamylase thermal inactivation constant. unlike amino acid substitution p128a ... | 2010 | 20391767 |
| influence of different substrates on the production of a mutant thermostable glucoamylase in submerged fermentation. | three mutations, ser54→pro, thr314→ala, and his415→tyr, were identified in aspergillus awamori glucoamylase gene expressed by saccharomyces cerevisiae. the mutant glucoamylase (ga) was substantially more thermostable than a wild-type ga at 70 °c, with a 3.0 kj mol(-1) increase in the free energy of thermo-inactivation. the effect of starch from different botanical sources on the production of this ga was measured in liquid fermentation using commercial soluble starch, cassava, potato, and corn a ... | 2011 | 20414741 |
| species distribution and in vitro azole susceptibility of aspergillus section nigri isolates from clinical and environmental settings. | aspergillus section nigri includes species of interest for animal and human health, although studies on species distribution are limited to human cases. data on the antifungal susceptibilities and the molecular mechanism of triazole resistance in strains belonging to this section are scant. forty-two black aspergillus strains from human patients (16 isolates), animals (14 isolates), and the environment (12 isolates) were molecularly characterized and their in vitro triazole susceptibilities inve ... | 2016 | 27413191 |
| valorization of bakery waste for biocolorant and enzyme production by monascus purpureus. | a concept of utilizing bakery waste as a nutrient source for the fermentative production of bio-colorant by monasucus purpureus has been developed. the proposed ideas provide an innovative approach to establish a system or method to reduce the bakery waste problem encountered by hong kong or other countries. bakery waste collected from bakery store was used in submerged and solid-state fermentation of monascus purpureus to produce bio-colorant, which could potentially be applied in food and text ... | 2016 | 27208753 |
| continuous biohydrogen production from waste bread by anaerobic sludge. | in this study, continuous biohydrogen production from waste bread by anaerobic sludge was performed. the waste bread was first hydrolyzed by the crude enzymes which were generated by aspergillus awamori and aspergillus oryzae via solid-state fermentation. it was observed that 49.78g/l glucose and 284.12mg/l free amino nitrogen could be produced with waste bread mass ratio of 15% (w/v). the waste bread hydrolysate was then used for biohydrogen production by anaerobic sludge in a continuous stirre ... | 2016 | 27065225 |
| aspergillus tubingensis and aspergillus niger as the dominant black aspergillus, use of simple pcr-rflp for preliminary differentiation. | this work aimed to identify the species distribution of common clinical and environmental isolates of black aspergilli based on simple restriction fragment length polymorphism (rflp) analysis of the β-tubulin gene. a total of 149 clinical and environmental strains of black aspergilli were collected and subjected to preliminary morphological examination. total genomic dnas were extracted, and pcr was performed to amplify part of the β-tubulin gene. at first, 52 randomly selected samples were spec ... | 2016 | 26852194 |
| biodegradation kinetics of thin-stillage treatment by aspergillus awamori and characterization of recovered chitosan. | an attempt has been made to provide solution for distillery wastewater using fungal pretreatment followed by an anaerobic process to achieve higher organic matter removal, which is a challenge at present with currently adopted technologies. submerged growth kinetics of distillery wastewater supernatant by aspergillus awamori was also evaluated. the proposed kinetic models using a logistic equation for fungal growth and the leudeking-piret equation for product formation were validated experimenta ... | 2016 | 26512009 |
| valorisation of mixed bakery waste in non-sterilized fermentation for l-lactic acid production by an evolved thermoanaerobacterium sp. strain. | in this study, an advanced biorefinery technology that uses mixed bakery waste has been developed to produce l-lactic acid using an adaptively evolved thermoanaerobacterium aotearoense la1002-g40 in a non-sterilized system. under these conditions, mixed bakery waste was directly hydrolysed by aspergillus awamori and aspergillus oryzae, resulting in a nutrient-rich hydrolysate containing 83.6g/l glucose, 9.5 g/l fructose and 612 mg/l free amino nitrogen. t. aotearoense la1002-g40 was evaluated an ... | 2015 | 26363501 |
| the method of integrated kinetics and its applicability to the exo-glycosidase-catalyzed hydrolysis of p-nitrophenyl glycosides. | in the present work we suggest an efficient method, using the whole time course of the reaction, whereby parameters kcat, km and product ki for the hydrolysis of a p-nitrophenyl glycoside by an exo-acting glycoside hydrolase can be estimated in a single experiment. its applicability was demonstrated for three retaining exo-glycoside hydrolases, β-xylosidase from aspergillus awamori, β-galactosidase from penicillium sp. and α-galactosidase from thermotoga maritima (tmgala). during the analysis of ... | 2015 | 26005928 |
| batch dark fermentation from enzymatic hydrolyzed food waste for hydrogen production. | a combination bioprocess of solid-state fermentation (ssf) and dark fermentative hydrogen production from food waste was developed. aspergillus awamori and aspergillus oryzae were utilized in ssf from food waste to generate glucoamylase and protease which were used to hydrolyze the food waste suspension to get the nutrients-rich (glucose and free amino nitrogen (fan)) hydrolysate. both glucose and fan increased with increasing of food waste mass ratio from 4% to 10% (w/v) and the highest glucose ... | 2015 | 25978853 |
| [changes in growth patterns and intracellular calcium concentrations in aspergillus awamori treated with amphotericin b]. | growth patterns and intracellular ca2+ concentrations in the mutant strain aspergillus awamori 66a, containing a recombinant aequorin gene were studied in the presence of a permeabilizing fungicidal agent amphotericin b. the cell response, i.e., changes in the growth and development of the fungus (initiation of spore germination, mycelial growth, and intensity ofsporulation) was dose-dependent. low concentrations of amphotericin b (2.5 microm) stimulated spore germination: the number of germinat ... | 2015 | 25844454 |
| effect of feeding aspergillus awamori and canola seed on the growth performance and muscle fatty acid profile in broiler chicken. | the aim of this study was to examine effects of dietary supplementation with aspergillus awamori and feeding canola seed on the growth and fatty acid profile in broilers. twenty-eight chicks (15 days old) were assigned to the following groups: (1) control, fed a basal diet; (2) awamori, fed the basal diet supplemented with 0.05% a. awamori; (3) canola, fed a diet containing 5% canola seed; and (4) canola + awamori, fed the canola diet supplemented with a. awamori (seven birds/group). body weight ... | 2015 | 25773115 |
| multivariate optimization and supplementation strategies for the simultaneous production of amylases, cellulases, xylanases, and proteases by aspergillus awamori under solid-state fermentation conditions. | the production of extracts containing a pool of enzymes for extensive biomass deconstruction can lead to significant advantages in biorefinery applications. in this work, a strain of aspergillus awamori ioc-3914 was used for the simultaneous production of five groups of hydrolases by solid-state fermentation of babassu cake. sequential experimental design strategies and multivariate optimization using the desirability function were first used to study temperature, moisture content, and granulome ... | 2015 | 25413792 |
| xylan degradation improved by a combination of monolithic columns bearing immobilized recombinant β-xylosidase from aspergillus awamori x-100 and grindamyl h121 β-xylanase. | synergistic action of exo- and endohydrolazes is preferred for effective destruction of biopolymers. the main purpose of the present work was to develop an efficient tool for degradation of xylan. macroporous lab-made monolithic columns and commercial cim-epoxy disk were used to immobilize the recombinant β-xylosidase from aspergillus awamori and grindamyl β-xylanase. the efficiency of xylan degradation using the low-loaded β-xylosidase column appeared to be four times higher than for the in-sol ... | 2015 | 25367775 |
| [introduction of additional thiol groups into glucoamylase in aspergillus awamori and their effect on the thermal stability and catalytic activity of the enzyme]. | five mutant forms of glucoamylase (ga) from the filamentous fungus aspergillus awamori with artificial disulfide bonds (4d-g137a\a14c, 6d-a14c\y419c\g137a, 10d-v13c\g396c, 11d-v13c\g396c\a14c\y419c\g137a, and 20d-g137a\a246c\a14c) were constructed using computer simulation and experimentally tested for thermostability. the introduction of two additional disulfide bonds between its first and thirteenth alpha-helices and that of the loop located close to a catalytic residue--e400--made it possible ... | 2014 | 25272730 |
| formulation of fermentation media from flour-rich waste streams for microbial lipid production by lipomyces starkeyi. | flour-rich waste (frw) and by-product streams generated by bakery, confectionery and wheat milling plants could be employed as the sole raw materials for generic fermentation media production, suitable for microbial oil synthesis. wheat milling by-products were used in solid state fermentations (ssf) of aspergillus awamori for the production of crude enzymes, mainly glucoamylase and protease. enzyme-rich ssf solids were subsequently employed for hydrolysis of frw streams into nutrient-rich ferme ... | 2014 | 25150217 |
| mixed food waste as renewable feedstock in succinic acid fermentation. | mixed food waste, which was directly collected from restaurants without pretreatments, was used as a valuable feedstock in succinic acid (sa) fermentation in the present study. commercial enzymes and crude enzymes produced from aspergillus awamori and aspergillus oryzae were separately used in hydrolysis of food waste, and their resultant hydrolysates were evaluated. for hydrolysis using the fungal mixture comprising a. awamori and a. oryzae, a nutrient-complete food waste hydrolysate was genera ... | 2014 | 25149459 |
| structural analysis of cerebrosides from aspergillus fungi: the existence of galactosylceramide in a. oryzae. | glucosylceramide and galactosylceramide were detected in three aspergillus species: aspergillus oryzae, aspergillus sojae and aspergillus. awamori, using borate-coated tlc. the cerebrosides from a. oryzae were further purified by ion exchange and iatrobeads column chromatographies with or without borate, and determined the composition of sugar, fatty acid and sphingoid base by gc/ms, maldi-tof/ms and (1)h-nmr. we identified them as β-glucosylceramide and β-galactosylceramide. the ceramide moiety ... | 2014 | 25129050 |
| biotransformed soybean extract (bse) inhibits melanoma cell growth and viability in vitro: involvement of nuclear factor-kappa b signaling. | melanoma is recognized as one of the most aggressive cancers with a relatively high propensity for metastasis. the prognosis of melanoma remains poor in spite of treatment advances, emphasizing the importance of additional preventive measures. isoflavonoids have become not only potential chemopreventive, but also important therapeutic natural agents. we evaluated the antiproliferative and proapoptotic properties of biotransformed soybean extract (bse) in a375 melanoma cells. previous analyses de ... | 2014 | 25072850 |
| upgrading the antioxidant potential of cereals by their fungal fermentation under solid-state cultivation conditions. | solid-state fermentation (ssf) at 30°c for 72 h with four generally recognized as safe (gras) filamentous fungi (aspergillus oryzae ncim 1212, aspergillus awamori mtcc no. 548, rhizopus oligosporus ncim 1215 and rhizopus oryzae rck2012) showed high efficiency for the improvement of water-soluble total phenolic content (tpc) and antioxidant properties including abts(●+) [2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid)] and dpph(●) (2,2'-diphenyl-1-picrylhydrazyl) scavenging capacities of ... | 2014 | 24964826 |
| enhancing organic matter removal, biopolymer recovery and electricity generation from distillery wastewater by combining fungal fermentation and microbial fuel cell. | for enhancing organic matter removal from cereal-based distillery stillage two-stage treatment consisting of fermentation by aspergillus awamori followed by microbial fuel cell (mfc) is proposed. considerable reduction in total and soluble chemical oxygen demand (cod) up to 70% and 40%, respectively, along with 98% reduction of suspended solids (ss) has been achieved during fungal pretreatment. the process generated chitosan, a useful fermentation byproduct from fungal mycelia, as 0.6-0.7g/l of ... | 2015 | 25460978 |
| effects of an aglycone-rich biotransformed soybean extract in human endothelial cells. | estrogen replacement therapy is not thought to be a safe treatment for prevention of cardiovascular disease in menopausal women; isoflavones are a possible alternative. estrogen produces beneficial effects on the cardiovascular system by enhancing production of nitric oxide, a vasoprotective and antiatherosclerotic agent. estrogen-like compounds such as isoflavones are also suggested for increasing nitric oxide production. isoflavones are present mainly in soy foods as glucosides, but soy isofla ... | 2015 | 25530207 |
| fungal hydrolysis in submerged fermentation for food waste treatment and fermentation feedstock preparation. | potential of fungal hydrolysis in submerged fermentation by aspergillus awamori and aspergillus oryzae as a food waste treatment process and for preparation of fermentation feedstock has been investigated. by fungal hydrolysis, 80-90% of the initial amount of waste was reduced and degraded within 36-48 h into glucose, free amino nitrogen (fan) and phosphate. experiments revealed that 80-90% of starch can be converted into glucose and highest concentration of fan obtained, when solid mashes of a. ... | 2014 | 24583214 |
| intensification of fructosyltransferases and fructo-oligosaccharides production in solid state fermentation by aspergillus awamori ghrts. | the present work was aimed to investigate the impact of the solid substrates mixture on fructosyltransferases (ftase) and fructo-oligosaccharides (fos) production. an augmented simplex lattice design was used to optimize a three component mixture for ftase production. among selected substrates corn cobs has highest impact on ftase production followed by wheat bran and rice bran. all two substrates and three substrate combinations showed the highest enzyme production than their individual levels. ... | 2013 | 24426134 |
| untreated chlorella homosphaera biomass allows for high rates of cell wall glucan enzymatic hydrolysis when using exoglucanase-free cellulases. | chlorophyte microalgae have a cell wall containing a large quantity of cellulose iα with a triclinic unit cell hydrogen-bonding pattern that is more susceptible to hydrolysis than that of the cellulose iβ polymorphic form that is predominant in higher plants. this study addressed the enzymatic hydrolysis of untreated chlorella homosphaera biomass using selected enzyme preparations, aiming to identify the relevant activity profile for the microalgae cellulose hydrolysis. enzymes from acremonium c ... | 2015 | 25763103 |
| transformation of litchi pericarp-derived condensed tannin with aspergillus awamori. | condensed tannin is a ubiquitous polyphenol in plants that possesses substantial antioxidant capacity. in this study, we have investigated the polyphenol extraction recovery and 2,2-diphenyl-1-picrylhydrazyl (dpph) scavenging activity of the extracted polyphenol after litchi pericarp is treated with aspergillus awamori, aspergillus sojae or aspergillus oryzae. we have further explored the activity of a. awamori in the formation of condensed tannin. the treatment of a. awamori appeared to produce ... | 2016 | 27420043 |
| biohydrogen production in the suspended and attached microbial growth systems from waste pastry hydrolysate. | waste pastry was hydrolyzed by glucoamylase and protease which were obtained from solid state fermentation of aspergillus awamori and aspergillus oryzae to produce waste pastry hydrolysate. then, the effects of hydraulic retention times (hrts) (4-12h) on hydrogen production rate (hpr) in the suspended microbial growth system (continuous stirred tank reactor, cstr) and attached microbial growth system (continuous mixed immobilized sludge reactor, cmisr) from waste pastry hydrolysate were investig ... | 2016 | 27416509 |
| antidiabetic potential of a peptide isolated from an endophytic aspergillus awamori. | to exploit the potential of endophytic fungi for pharmaceutically important antidiabetic alpha glycosidase inhibitors. | 2016 | 26544796 |
| a 3-vinyl cephem derivative, a useful intermediate in the synthesis of cepham antibiotics, from aspergillus awamori associated with banana fruit. | aspergillus awamori was isolated from a diseased banana fruit, musa acuminata cv. ambul. the fungus was fermented in potato dextrose broth and on potato dextrose agar media and the fungal media were extracted with etoac. chromatographic separation of the etoac extracts furnished 4-methoxybenzyl 7-phenylacetamido-3-vinyl-3-cephem-4-carboxylate (1), along with three naphtho-γ-pyrones, flavasperone (2), foncesinone a (3) and aurasperone a (4), and three alkaloids, aspernigrin a (5), pestalamide c ( ... | 2015 | 26669099 |
| immobilization of halophilic aspergillus awamori em66 exochitinase on grafted k-carrageenan-alginate beads. | a novel extreme halophilic exochitinase enzyme was produced by honey isolate aspergillus awamori em66. the enzyme was immobilized successfully on k-carrageenan-alginate gel carrier (ca) with 93 % immobilization yield. the immobilization process significantly improved the enzyme specific activity 2.6-fold compared to the free form. the significant factors influencing the immobilization process such as enzyme protein concentration and loading time were studied. distinguishable characteristics of o ... | 2016 | 28330097 |
| optimization of d-lactic acid production using unutilized biomass as substrates by multiple parallel fermentation. | this study investigated the optimization of d-lactic acid production from unutilized biomass, specifically banana peel and corncob by multiple parallel fermentation (mpf) with leuconostoc mesenteroides and aspergillus awamori. the factors involved in mpf that were assessed in this study comprised banana peel and corncob, kh2po4, tween 80, mgso4·7h2o, nacl, yeast extract, and diammonium hydrogen citrate to identify the optimal concentration for d-lactic acid production. optimization of these comp ... | 2016 | 28330258 |
| prospecting for the incidence of genes involved in ochratoxin and fumonisin biosynthesis in brazilian strains of aspergillus niger and aspergillus welwitschiae. | aspergillus niger "aggregate" is an informal taxonomic rank that represents a group of species from the section nigri. among a. niger "aggregate" species aspergillus niger sensu stricto and its cryptic species aspergillus welwitschiae (=aspergillus awamori sensu perrone) are proven as ochratoxin a and fumonisin b2 producing species. a. niger has been frequently found in tropical and subtropical foods. a. welwitschiae is a new species, which was recently dismembered from the a. niger taxon. these ... | 2016 | 26803270 |
| determination and optimization of vitamin b complex in xylanase enzyme treated polished rice by response surface methodology. | the present study provides information about the concentrations of vitamin b (thiamine, riboflavin, pyridoxine and niacin) in polished brown rice treated with xylanase. xylanase enzyme was produced from aspergillus awamori mtcc 9166. brown rice was treated with 60-100% enzyme (40 ml of buffer -undiluted) for 30 to 150 min (with variation of 30 min) at 30 degrees c to 50 degrees c (with variation of 5 degrees c) to attain a saturated moisture level of 35.5 g100(-1)g .the enzyme acted upon selecti ... | 2016 | 27498499 |
| phenol biodegradation by fungal cells immobilized in sol-gel hybrids. | the capability of cells of the fungus aspergillus awamori, either free or immobilized in hybrid sol-gel material cells, for phenol biodegradation was demonstrated. phenol was present in the reaction mixture as the sole carbon and energy source, and its decomposition was followed in repeated batch degradation experiments. atomic force microscopy provided information on the development of self-organizing structures in the materials synthesized by the sol-gel method. phenol biodegradation was media ... | 2013 | 23659173 |
| food waste as nutrient source in heterotrophic microalgae cultivation. | glucose, free amino nitrogen (fan), and phosphate were recovered from food waste by fungal hydrolysis using aspergillus awamori and aspergillus oryzae. using 100g food waste (dry weight), 31.9 g glucose, 0.28 g fan, and 0.38 g phosphate were recovered after 24h of hydrolysis. the pure hydrolysate has then been used as culture medium and nutrient source for the two heterotrophic microalgae schizochytrium mangrovei and chlorella pyrenoidosa, s. mangrovei and c. pyrenoidosa grew well on the complex ... | 2013 | 23587816 |
| distribution and mycotoxigenic potential of aspergillus section nigri species in naturally contaminated almonds. | in a previous study, inedible almond pick-out samples were assayed for aflatoxin and aflatoxigenic aspergillus species. these samples contained high populations of black-spored aspergillus section nigri species. to investigate whether these species may contribute to the total potential mycotoxin content of almonds, aspergillus section nigri strains were isolated from these samples and assayed for ochratoxin a (ota) and fumonisin b2 (fb2). the majority of isolates (117 strains, 68%) were identifi ... | 2013 | 23575138 |
| aspergillus awamori-fermented mung bean seed coats enhance the antioxidant and immune responses of weaned pigs. | the potential benefits of aspergillus-fermented mung bean seed coats (fmsc) for weaned pigs remain unexplored. both in vitro and in vivo experiments were employed to evaluate the potential of fmsc supplement on the growth, antioxidant and immune responses of weaned pigs. the total polyphenols and dpph scavenging capability of ethanol extract of fmsc exhibited a greater (p < 0.01) increase than those of pre-fermentation. with the addition of the polyphenol of fmsc extract, an increase in phagocyt ... | 2017 | 28063238 |
| fermentative polyhydroxybutyrate production from a novel feedstock derived from bakery waste. | in this study, halomonas boliviensis was cultivated on bakery waste hydrolysate and seawater in batch and fed-batch cultures for polyhydroxybutyrate (phb) production. results demonstrated that bakery waste hydrolysate and seawater could be efficiently utilized by halomonas boliviensis while phb contents between 10 and 30% (w/w) were obtained. furthermore, three methods for bakery waste hydrolysis were investigated for feedstock preparation. these include: (1) use of crude enzyme extracts from as ... | 2014 | 25136626 |
| enhancing the antitumor cell proliferation and cu(2+)-chelating effects of black soybeans through fermentation with aspergillus awamori. | in the present study, black soybeans were fermented with aspergillus awamori at 30°c for 3 days. the effect of fermentation on the antiproliferative effect against human colon cancer cells, caco-2 and ht-29 as well as cu(2+)-chelating effect of black soybeans was investigated. it was found that the water, 80% methanol or 80% ethanol extract of fermented black soybeans showed a significantly higher (p < 0.05) antiproliferative and cu(2+)-chelating effect than did the respective extract of non-fer ... | 2013 | 23177213 |
| enhanced dpph radical scavenging activity and dna protection effect of litchi pericarp extract by aspergillus awamori bioconversion. | | 2012 | 23016522 |
| multiresponse optimization of inoculum conditions for the production of amylases and proteases by aspergillus awamori in solid-state fermentation of babassu cake. | this work aimed at investigating the simultaneous production of amylases and proteases by solid-state fermentation (ssf) of babassu cake using aspergillus awamori ioc-3914. by means of experimental design techniques and the desirability function, optimum inoculum conditions (c/n ratio of propagation medium, inoculum age, and concentration of inoculum added to ssf medium) for the production of both groups of enzymes were found to be 25.8, 28.4 h, and 9.1 mg g(-1), respectively. significant influe ... | 2011 | 21915371 |
| feeding aspergillus awamori reduces skeletal muscle protein breakdown and stimulates growth in broilers. | this study was conducted to show that dietary supplementation of a fungus, aspergillus awamori called koji in japan, reduces skeletal muscle protein breakdown and stimulates growth in broiler chickens. a total of 30 chicks at 15 days of age was divided into control and two treatment groups (10 birds per treatment). control group was fed basal diet and treatment groups were fed the basal diets supplemented with a. awamori at levels of 0.05% and 0.2%. the birds were raised for 12 days from 15 to 2 ... | 2012 | 22862929 |
| Aspergillus niger contains the cryptic phylogenetic species A. awamori. | Aspergillus section Nigri is an important group of species for food and medical mycology, and biotechnology. The Aspergillus niger 'aggregate' represents its most complicated taxonomic subgroup containing eight morphologically indistinguishable taxa: A. niger, Aspergillus tubingensis, Aspergillus acidus, Aspergillus brasiliensis, Aspergillus costaricaensis, Aspergillus lacticoffeatus, Aspergillus piperis, and Aspergillus vadensis. Aspergillus awamori, first described by Nakazawa, has been compar ... | 2011 | 22036292 |
| Development of an efficient gene-targeting system in Aspergillus luchuensis by deletion of the non-homologous end joining system. | The industrial fungus Aspergillus luchuensis is used to produce a distilled spirit in Okinawa Island, Japan. Recently, the genome sequence of A. luchuensis RIB2604 (Aspergillus awamori NBRC 4314) was revealed and many functional genes are now expected to be analyzed. Gene targeting is necessary for analyzing the function of a gene; however, gene targeting frequencies in A. luchuensis are very low. To develop a highly efficient gene-targeting system for A. luchuensis, we disrupted A. luchuensis l ... | 2011 | 21903470 |
| Species assignment and antifungal susceptibilities of black aspergilli recovered from otomycosis cases in Iran. | Black aspergilli are among the main causative agents of otomycosis worldwide. In this study, the species assignment of black aspergilli isolated from otomycosis cases in Iran was carried out using sequence analysis of part of the calmodulin gene. The results indicate that Aspergillus niger is not the only black Aspergillus species involved in otomycosis cases in Iran: Aspergillus awamori and Aspergillus tubingensis are also able to cause ear infections. Antifungal susceptibility tests were carri ... | 2011 | 21895787 |
| studies on organic and in-organic biostimulants in bioremediation of diesel-contaminated arable soil. | in this study, use of inorganic fertilizer (n.p.k) was compared with organic manure (compost) in the bioremediation of diesel-polluted agricultural soil over a two-month period. renewal by enhanced natural attenuation was used as control. the results revealed that total petroleum hydrocarbon removal from polluted soil was 71.40 ± 5.60% and 93.31 ± 3.60% for n.p.k and compost amended options, respectively. the control (natural attenuation) had 57.90 ± 3.98% of total petroleum hydrocarbon removed. ... | 2016 | 27494315 |
| garcina cambogia leaf and seawater for tannase production by marine aspergillus awamori btmfw032 under slurry state fermentation. | garcinia gummi-gutta (syn. g. cambogia, g. quaesita), known to have medicinal properties, was evaluated as a substrate and inducer for tannase production by a marine aspergillus awamori btmfw032, under slurry state fermentation using czapekdox-minimal medium and sea water as the cultivation medium. among the various natural tannin substrates evaluated, garcinia leaf supported maximal tannase production. the cultivation conditions and components of the cultivation medium were optimized employing ... | 2011 | 22312743 |
| lipase from marine aspergillus awamori btmfw032: production, partial purification and application in oil effluent treatment. | marine fungus btmfw032, isolated from seawater and identified as aspergillus awamori, was observed to produce an extracellular lipase, which could reduce 92% fat and oil content in the effluent laden with oil. in this study, medium for lipase production under submerged fermentation was optimized statistically employing response surface method toward maximal enzyme production. medium with soyabean meal-0.77% (w/v); (nh(4))(2)so(4)-0.1m; kh(2)po(4)-0.05m; rice bran oil-2% (v/v); cacl(2)-0.05m; peg ... | 2011 | 21549226 |
| raw starch fermentation to ethanol by an industrial distiller's yeast strain of saccharomyces cerevisiae expressing glucoamylase and α-amylase genes. | industrial strains of a polyploid, distiller's saccharomyces cerevisiae that produces glucoamylase and α-amylase was used for the direct fermentation of raw starch to ethanol. strains contained either aspergillus awamori glucoamylase gene (ga1), debaryomyces occidentalis glucoamylase gene (gam1) or d. occidentalis α-amylase gene (amy), singly or in combination, integrated into their chromosomes. the strain expressing both ga1 and amy generated 10.3% (v/v) ethanol (80.9 g l(-1)) from 20% (w/v) ra ... | 2011 | 21479627 |
| fermentation with aspergillus awamori enhanced contents of amino nitrogen and total phenolics as well as the low-density lipoprotein oxidation inhibitory activity of black soybeans. | a solid fermentation was performed on black soybeans with aspergillus awamori. the effects of fermentation on the contents of total phenolics and amino nitrogen and on the inhibitory effect on low-density lipoprotein (ldl) oxidation of black soybeans were examined. results revealed that fermentation significantly enhanced the ldl oxidation inhibitory activity and total phenolics and amino nitrogen contents of black soybeans. the increased content of amino nitrogen was closely related to the enha ... | 2011 | 21370926 |
| propyl gallate synthesis using acidophilic tannase and simultaneous production of tannase and gallic acid by marine aspergillus awamori btmfw032. | marine aspergillus awamori btmfw032, recently reported by us, produce acidophilic tannase as extracellular enzyme. here, we report the application of this enzyme for synthesis of propyl gallate by direct transesterification of tannic acid and in tea cream solubilisation besides the simultaneous production of gallic acid along with tannase under submerged fermentation by this fungus. this acidophilic tannase enabled synthesis of propyl gallate by direct transesterification of tannic acid using pr ... | 2011 | 21279470 |
| production and characterization of glucoamylase from fungus aspergillus awamori expressed in yeast saccharomyces cerevisiae using different carbon sources. | glucoamylase is widely used in the food industry to produce high glucose syrup, and also in fermentation processes for production beer and ethanol. in this work the productivity of the glucoamylase of aspergillus awamori expressed by the yeast saccharomyces cerevisiae, produced in submerged fermentation using different starches, was evaluated and characterized physico-chemically. the enzyme presented high specific activity, 13.8 u/mgprotein or 2.9 u/mgbiomass, after 48 h of fermentation using so ... | 2008 | 24031189 |
| three different types of α-amylases from aspergillus awamori kt-11: their purifications, properties, and specificities. | three forms of α-amylases, designated amyl i, amyl ii, and amyl iii were purified to a homogenous state by several column chromatographies from a koji culture in wheat bran of a strain of black mold, which was isolated in indonesia and identified as aspergillus awamori. they have molecular weights of 49,000, 63,000, and 97,000 by sds-page, respectively, and the optimum phs were 4.0 for amyl i and 5.5 for both amyl ii and amyl iii on soluble starch. amyl i hydrolyzed malto-tetraose, -pentaose, -h ... | 1998 | 27396998 |
| [increase in glucoamylase productivity of aspergillus awamori strain by combination of radiating mutagenesis and plasmid transformation methods]. | increase in the level of amylolytic genes activator protein encoded by amyr gene was shown to result in enhancement of glucoamylase productivity of a. awamori strain by 30%. however, the same effect equal to 30% increase can be achieved by introduction of extra copies of gla gene encoding glucoamylase. these two effects were not additive, which gave the possibility to suggest an additional limitation in the egulation mechanism of glucoamylase gene expression in aspergillus family strains while i ... | 2010 | 21261079 |
| antifungal activity and identification of lactobacilli, isolated from traditional dairy product "katak". | filamentous moulds are the main spoilage microorganisms, responsible for significant economic losses and several healthy risks in human food chain. the lactic acid bacteria (lab), especially lactobacilli could be a natural antagonist of these dangerous organisms. in bulgaria, a very limited data exists on the antifungal activity of lab microbiota of fermented dairy products. in the present study, four active strains were isolated from traditional fermented curd/yogurt-like product "katak", produ ... | 2014 | 24887637 |
| xylan-hydrolysing enzymes from thermophilic and mesophilic fungi. | screening of 40 mesophilic and 13 thermophilic fungi indicated that enzyme activities capable of degrading oat spelt xylan extensively were produced by only a few of the mesophilic species investigated. the relatively low degree of hydrolysis effected by the enzymes from thermophilic organisms could be explained, in part, by their lack of β-xylosidase. several strains of aspergillus awamori and aspergillus phoenicis were notable in producing high xylanase and β-xylosidase and low protease activi ... | 1991 | 24425133 |
| comparison of species composition and fumonisin production in aspergillus section nigri populations in maize kernels from usa and italy. | fumonisin contamination of maize is considered a serious problem in most maize-growing regions of the world, due to the widespread occurrence of these mycotoxins and their association with toxicosis in livestock and humans. fumonisins are produced primarily by species of fusarium that are common in maize grain, but also by some species of aspergillus sect. nigri, which can also occur on maize kernels as opportunistic pathogens. understanding the origin of fumonisin contamination in maize is a ke ... | 2014 | 25087207 |
| application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification. | polygalacturonase and α-amylase play vital role in fruit juice industry. in the present study, polygalacturonase was produced by aspergillus awamori nakazawa mtcc 6652 utilizing apple pomace and mosambi orange (citrus sinensis var mosambi) peels as solid substrate whereas, α-amylase was produced from a. oryzae (ifo-30103) using wheat bran by solid state fermentation (ssf) process. these carbohydrases were decolourized and purified 8.6-fold, 34.8-fold and 3.5-fold, respectively by activated charc ... | 2014 | 24948919 |
| integrative effects of feeding aspergillus awamori and fructooligosaccharide on growth performance and digestibility in broilers: promotion muscle protein metabolism. | this study was conducted to show the effect of aspergillus awamori (aa), fructooligosaccharide (fos), and combined aspergillus awamori and fructooligosaccharide (aa + fos) on growth, digestibility, blood parameters, and expression of some growth-related genes. a total of 60 broiler chicks at the age of 15 d were divided into a control group (n = 15) and 3 treatment groups. the control group was fed a basal diet, and the treatment groups were fed basal diets supplemented with 0.05% aa, 0.05% fos, ... | 2014 | 24895630 |
| operating conditions for the continuous bioremediation of free cyanide contaminated wastewater using aspergillus awamori. | generation of cyanide-containing wastewater is a growing problem worldwide as numerous cyanide complexes are highly unstable and degrade to form free cyanide (f-cn), the most toxic form of cyanide. agro-waste materials, such as sweet orange (citrus sinensis) waste from the citrus industry, are rich in readily metabolisable carbohydrates that can supplement microbial activity and thus support biodegradation of toxic compounds in wastewater. this study reports on optimal operating conditions for t ... | 2014 | 24622547 |
| modern taxonomy of biotechnologically important aspergillus and penicillium species. | taxonomy is a dynamic discipline and name changes of fungi with biotechnological, industrial, or medical importance are often difficult to understand for researchers in the applied field. species belonging to the genera aspergillus and penicillium are commonly used or isolated, and inadequate taxonomy or uncertain nomenclature of these genera can therefore lead to tremendous confusion. misidentification of strains used in biotechnology can be traced back to (1) recent changes in nomenclature, (2 ... | 2014 | 24377856 |
| comparative study of biochemical properties of glucoamylases from the filamentous fungi penicillium and aspergillus. | here we report the first isolation to homogeneous forms of two glucoamylases from the fungus penicillium verruculosum and their study in comparison with known glucoamylases from aspergillus awamori and aspergillus niger. genes that encode glucoamylases from p. verruculosum were cloned and expressed in the fungus penicillium canescens, and the recombinant glucoamylases were obtained with subsequent study of their molecular weights, isoelectric points, optimal temperature and ph values, and stabil ... | 2013 | 24237153 |
| production of quercetin, kaempferol and their glycosidic derivatives from the aqueous-organic extracted residue of litchi pericarp with aspergillus awamori. | our previous work exhibited aspergillus awamori fermentation of the litchi pericarp increased significantly antioxidant activity and dna protection effect. in this present study, the litchi pericarp and its aqueous-organic extracted residues were fermented by a. awamori in order to elucidate the enhanced beneficial effects. the study identified that rutin which present in litchi pericarp could be deglycosylated to form quercetin and quercetin-3-glucoside after the fermentation. application the s ... | 2014 | 24128471 |
| characterization of antifungal activity of the gh-46 subclass iii chitosanase from bacillus circulans mh-k1. | we characterized the antifungal activity of the bacillus circulans subclass iii mh-k1 chitosanase (mh-k1 chitosanase), which is one of the most intensively studied glycoside hydrolases (ghs) that belong to gh family 46. mh-k1 chitosanase inhibited the growth of zygomycetes fungi, rhizopus and mucor, even at 10 pmol (0.3 μg)/ml culture probably via its fungistatic effect. the amino acid substitution e37q abolished the antifungal activity of mh-k1 chitosanase, but retained binding to chitotriose. ... | 2013 | 23892828 |
| aspergillus awamori feeding modifies lipid metabolism in rats. | in the present study, an experiment was conducted to show that a. awamori modifies lipid metabolism in mammals. a total number of 24 rats at 6 weeks of age were divided into 2 groups (10% and 30% fat dietary groups), and each group was further divided into control and experimental groups (6 rats per group). rats in the experimental groups were given diets containing 0.05% a. awamori. the diets were administered for 3 weeks to evaluate the effects of a. awamori on growth, plasma lipid profile, an ... | 2013 | 23841078 |
| [influence of uv-radiation on physical-chemical and functional properties of free immobilized glucoamylase from aspergillus awamori]. | the effect of uv light (240-390 nm) at the doses of 151-1510 j/m2 on the catalytic activity of free and im- mobilized glucoamylase from aspergillus awamori was investigated. it was established that the loss of catalytic activity of the enzyme was associated with photochemical transformations of trp-120, which is part of the active site of glucoamylase. it was shown that the use of collagen as a carrier for immobilization of glucoamylase reduces the constant photoinactivation of the enzyme. it wa ... | 2016 | 25507622 |
| [influence of uv-radiation on physical-chemical and functional properties of free immobilized glucoamylase from aspergillus awamori]. | the effect of uv light (240-390 nm) at the doses of 151-1510 j/m2 on the catalytic activity of free and im- mobilized glucoamylase from aspergillus awamori was investigated. it was established that the loss of catalytic activity of the enzyme was associated with photochemical transformations of trp-120, which is part of the active site of glucoamylase. it was shown that the use of collagen as a carrier for immobilization of glucoamylase reduces the constant photoinactivation of the enzyme. it wa ... | 2016 | 25427372 |
| improved growth of lactobacillus bulgaricus and streptococcus thermophilus as well as increased antioxidant activity by biotransforming litchi pericarp polysaccharide with aspergillus awamori. | this study was conducted to increase the bioactivity of litchi pericarp polysaccharides (lpps) biotransformed by aspergillus awamori. compared to the non-a. awamori-fermented lpp, the growth effects of a. awamori-fermented lpp on lactobacillus bulgaricus and streptococcus thermophilus were four and two times higher after 3 days of fermentation, respectively. increased 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity and dna protection activity of litchi pericarp polysaccharides were als ... | 2013 | 23484117 |
| burdock fermented by aspergillus awamori elevates cecal bifidobacterium, and reduces fecal deoxycholic acid and adipose tissue weight in rats fed a high-fat diet. | this study investigated the effects of dietary supplementation with burdock powder and aspergillus awamori-fermented burdock powder at 5% on the intestinal luminal environment and body fat in rats fed a high-fat (hf) diet. food intake and growth were unaffected by dietary manipulation. consumption of the burdock and fermented burdock diets significantly elevated fecal iga and mucins (indices of intestinal immune and barrier functions) and reduced fecal lithocholic acid (a risk factor for colon c ... | 2013 | 23291748 |
| efficacy of root-associated fungi and pgpr on the growth of pisum sativum (cv. arkil) and reproduction of the root-knot nematode meloidogyne incognita. | the effects of root-associated fungi (aspergillus awamori and glomus mosseae) and plant growth promoting rhizobacteria (pgpr) (pseudomonas putida, pseudomonas alcaligenes and paenibacillus polymyxa) were studied alone and in combination in glasshouse experiments on the growth of pea, enzyme activity (peroxidase and catalase) and reproduction of root-knot nematode meloidogyne incognita. application of a. awamori, g. mosseae and pgpr caused a significant increase in pea growth and enzyme activitie ... | 2013 | 22915081 |
| functional assembly and characterization of a modular xylanosome for hemicellulose hydrolysis in yeast. | five trimeric xylanosomes were successfully assembled on the cell surface of saccharomyces cerevisiae. three dockerin-tagged fungal enzymes, an endoxylanase (xynac) from thermomyces lanuginosus, a β-xylosidase (xlndt) from aspergillus niger and an acetylxylan esterase (awaxef) from aspergillus awamori, were displayed for the synergistic saccharification of birchwood xylan. the surface-expression scaffoldins were modular constructs with or without carbohydrate binding modules from thermotoga mari ... | 2013 | 22806709 |
| bioaugmented composting of jatropha de-oiled cake and vegetable waste under aerobic and partial anaerobic conditions. | this study was conducted to assess the effect of microbial inoculation in jatropha cake composting with different vegetable waste. the microbial inoculums composed of fungal strains (aspergillus awamori, aspergillus nidulans, trichoderma viride, phanerochaete chrysosporium) and bacterial inoculums (pseudomonas striata as phosphorus solublizer and azotobacter chroococcum as nitrogen fixer) were added to the compost mixture after the thermophilic phase was over for bioaugmenting of jatropha cake u ... | 2013 | 22736484 |