| cyclospora infection linked to travel to mexico, june to september 2015. | cyclospora cayetanensis was identified in 176 returned travellers from the riviera maya region of mexico between 1 june and 22 september 2015; 79 in the united kingdom (uk) and 97 in canada. uk cases completed a food exposure questionnaire. this increase in reported cyclospora cases highlights risks of gastrointestinal infections through travelling, limitations in cyclospora surveillance and the need for improved hygiene in the production of food consumed in holiday resorts. | 2015 | 26536814 |
| molecular detection of cyclospora in water, soil, vegetables and humans in southern italy signals a need for improved monitoring by health authorities. | to date, in europe, there is scant information on the occurrence of cyclospora in water from treatment plants and in humans, and no data are available on soil or fresh plant products. here, we undertook the first molecular survey of cyclospora in multiple biological matrices collected from the apulia region of southern italy. samples of irrigation water from four municipal treatment plants, eight different types of vegetables or fruit (cucumber, lettuce, fennel, celery, tomato, melon, endive and ... | 2015 | 26188495 |
| genetic similarities between cyclospora cayetanensis and cecum-infecting avian eimeria spp. in apicoplast and mitochondrial genomes. | cyclospora cayetanensis is an important cause for diarrhea in children in developing countries and foodborne outbreaks of cyclosporiasis in industrialized nations. to improve understanding of the basic biology of cyclospora spp. and development of molecular diagnostic tools and therapeutics, we sequenced the complete apicoplast and mitochondrial genomes of c. cayetanensis. | 2015 | 26152563 |
| molecular characterization of clinical isolates of cyclospora cayetanensis from patients with diarrhea in india. | cyclospora cayetanensis is an intestinal coccidian protozoan that has emerged as an important cause of both epidemic and endemic protracted diarrhea worldwide. though humans appear to be the only natural hosts; the role of animals as natural reservoir is uncertain but of increasing concern. the present study aimed to study the prevalence of coccidian in different groups such as immunocompromised, clinically apparent immunocompetent and healthy individuals. also, the study isolates were assessed ... | 2015 | 26068334 |
| the complete mitochondrial genome of the foodborne parasitic pathogen cyclospora cayetanensis. | cyclospora cayetanensis is a human-specific coccidian parasite responsible for several food and water-related outbreaks around the world, including the most recent ones involving over 900 persons in 2013 and 2014 outbreaks in the usa. multicopy organellar dna such as mitochondrion genomes have been particularly informative for detection and genetic traceback analysis in other parasites. we sequenced the c. cayetanensis genomic dna obtained from stool samples from patients infected with cyclospor ... | 2015 | 26042787 |
| infections by intestinal coccidia and giardia duodenalis. | the coccidians cryptosporidium spp, cyclospora cayetanensis, and cystoisospora belli and the flagellate giardia duodenalis are pathogenic protozoa associated with gastrointestinal manifestations. diagnosis relies heavily on microscopy, and although ova-and-parasite examinations can detect giardia and cystoisospora, cryptosporidium and cyclospora often require specific diagnostic requests. approved non-microscopy methods are available for giardia and cryptosporidium, although negative results are ... | 2015 | 26004650 |
| detection of cyclospora in captive chimpanzees and macaques by a quantitative pcr-based mutation scanning approach. | cyclospora is a protistan parasite that causes enteritis in several species of animals including humans. the aim of this study was to investigate the presence of cyclospora in captive non-human primates. | 2015 | 25972100 |
| 2013 multistate outbreaks of cyclospora cayetanensis infections associated with fresh produce: focus on the texas investigations. | the 2013 multistate outbreaks contributed to the largest annual number of reported us cases of cyclosporiasis since 1997. in this paper we focus on investigations in texas. we defined an outbreak-associated case as laboratory-confirmed cyclosporiasis in a person with illness onset between 1 june and 31 august 2013, with no history of international travel in the previous 14 days. epidemiological, environmental, and traceback investigations were conducted. of the 631 cases reported in the multista ... | 2015 | 25865140 |
| a linear mitochondrial genome of cyclospora cayetanensis (eimeriidae, eucoccidiorida, coccidiasina, apicomplexa) suggests the ancestral start position within mitochondrial genomes of eimeriid coccidia. | the near complete mitochondrial genome for cyclospora cayetanensis is 6184 bp in length with three protein-coding genes (cox1, cox3, cytb) and numerous lsrdna and ssrdna fragments. gene arrangements were conserved with other coccidia in the eimeriidae, but the c. cayetanensis mitochondrial genome is not circular-mapping. terminal transferase tailing and nested pcr completed the 5'-terminus of the genome starting with a 21 bp a/t-only region that forms a potential stem-loop. regions homologous to ... | 2015 | 25812835 |
| cyclospora cayetanensis travels in tap water on italian trains. | tap water samples from the toilets of an italian national railway train were collected over a period of 10 months and tested for the presence of cyclospora cayetanensis (c. cayetanensis) using evagreen® real-time polymerase chain reaction (rt-pcr) assay coupled with high resolution melting (hrm) analysis for protozoan detection and oocyst quantification. c. cayetanensis positive samples were detected in march, april, and may 2013, with the number of oocysts of 4, 5, and 11 per liter, respectivel ... | 2015 | 25719480 |
| identification and morphologic and molecular characterization of cyclospora macacae n. sp. from rhesus monkeys in china. | cyclospora spp. in nonhuman primates are most closely related to cyclospora cayetanensis, an emerging human pathogen causing outbreaks of cyclosporiasis in north america. studies thus far indicate the possible existence of host specificity in cyclospora spp. in this study, 411 fecal specimens from free-range rhesus monkeys (macaca mulatta) were collected and examined for cyclospora by sequence analysis of the small subunit rrna gene. a novel cyclospora species was identified in 28 (6.8%) specime ... | 2015 | 25673080 |
| detection of toxoplasma gondii and cyclospora cayetanensis in mytilus galloprovincialis from izmir province coast (turkey) by real time pcr/high-resolution melting analysis (hrm). | to evaluate the presence of toxoplasma gondii and cyclospora cayetanensis in edible shellfish, a total of 795 specimens of mytilus galloprovincialis from 8 different sites on the west coast of turkey (bays of izmir and mersin) were examined. fifty-three pooled samples were created and subjected to evagreen(®) realtime pcr assay and hrm analysis for protozoans detection and oocyst quantification. seven out of 8 sites were contaminated with at least one protozoan species. twenty-one out of 53 (39. ... | 2014 | 25084654 |
| [microsporidia in pediatric patients with leukemia or limphoma]. | microsporidia are intracellular micro-organisms, characterized by mature spores with chitin walls and by one extrusive polar tube through which they pour their sporoplasm to the host cells. in immunocompromised patients, enterocytozoon bieneusi and encephalitozoon intestinalis produce diarrhea and systemic dissemination. in mexico there is not information about microsporidia in children with cancer. | 2012 | 22690526 |
| population-based active surveillance for cyclospora infection--united states, foodborne diseases active surveillance network (foodnet), 1997-2009. | cyclosporiasis is an enteric disease caused by the parasite cyclospora cayetanensis. since the mid-1990 s, the centers for disease control and prevention has been notified of cases through various reporting and surveillance mechanisms. | 2012 | 22572662 |
| an outbreak of cyclosporiasis in 1996 associated with consumption of fresh berries- ontario. | a large foodborne outbreak of cyclosporiasis occurred in north america in 1996. an index cluster of cases associated with a catered event on may 11, 1996, in ontario sparked the recognition of this outbreak in canada. | 2000 | 18159270 |
| first report of tunisian coastal water contamination by protozoan parasites using mollusk bivalves as biological indicators. | in order to establish seawater contamination by emerging protozoan parasites, we used qpcr to molecularly characterize and evaluate the parasitic burden of giardia duodenalis, cryptosporidium spp., toxoplasma gondii, and cyclospora cayetanensis in 1255 wild bivalve mollusks collected along the tunisian coasts. t. gondii, g. duodenalis and c. cayetanensis were detected in 6.9% (99% ci=1.6-12.2%) pools of ruditapes decussatus. none of the samples were found positive to cryptosporidium spp.; 6.6% p ... | 2017 | 28168979 |
| cyclospora cayetanensis in a pediatric hospital in morelia, méxico. | cyclospora cayetanensis, a coccidian parasite, can cause gastrointestinal illness in humans and is characterized by watery and persistent diarrhea and abdominal pain. cyclosporiasis has been associated with traveler's diarrhea. the infection is acquired through food and waterborne transmission, particularly by consumption of contaminated fresh fruits and vegetables. in the present study, stool samples from 8,877 children were examined for ova and parasites at the pediatric hospital of morelia in ... | 2014 | 24957545 |
| cyclospora spp. in drills, bioko island, equatorial guinea. | | 2014 | 24565509 |
| recovery of cyclospora cayetanensis among asymptomatic rural thai schoolchildren. | to obtain the prevalence with clinical symptoms of cyclospora cayetanensis (c. cayetanensis), a coccidian protozoan parasite, in thailand which is the cause of an intestinal infection characterized by sporadic-to-frequent explosive diarrhea. | 2014 | 24461524 |
| cyclospora cayetanensis: this emerging protozoan pathogen in mexico. | a mexican airline pilot had clinical manifestations of illness after a five-day stay in lima, peru. six months later in mexico, he was given a diagnosis of infection with cyclospora cayetanensis by using coproparasitoscopic serial tests. he was treated twice with nitazoxadine successfully. | 2014 | 24379243 |
| evaluation of the easyscreen™ enteric parasite detection kit for the detection of blastocystis spp., cryptosporidium spp., dientamoeba fragilis, entamoeba complex, and giardia intestinalis from clinical stool samples. | the aim of this study was to evaluate the easyscreen™ enteric parasite detection kit (genetic signatures, sydney, australia) for the detection and identification of 5 common enteric parasites: blastocystis spp., cryptosporidium spp., dientamoeba fragilis, entamoeba complex, and giardia intestinalis in human clinical samples. a total of 358 faecal samples were included in the study. when compared to real-time pcr and microscopy, the easyscreen™ enteric parasite detection kit exhibited 92-100% sen ... | 2014 | 24286625 |
| intestinal parasitosis in school children of lalitpur district of nepal. | enteric parasites are the most common cause of parasitic diseases and cause significant morbidity and mortality, particularly in developing countries like nepal. the objective of this study was to estimate the prevalence and risk factors of intestinal parasitic infections among school going children of lalitpur district of nepal. | 2013 | 24207086 |
| detection of intestinal protozoa in the clinical laboratory. | despite recent advances in diagnostic technology, microscopic examination of stool specimens remains central to the diagnosis of most pathogenic intestinal protozoa. microscopy is, however, labor-intensive and requires a skilled technologist. new, highly sensitive diagnostic methods have been developed for protozoa endemic to developed countries, including giardia lamblia (syn. g. intestinalis/g. duodenalis) and cryptosporidium spp., using technologies that, if expanded, could effectively comple ... | 2014 | 24197877 |
| outbreaks of cyclosporiasis--united states, june-august 2013. | during june-august 2013, cdc, state and local public health officials, and the food and drug administration (fda) investigated an unusually large number of reports of cyclosporiasis (compared with annual reports to the national notifiable disease surveillance system [e.g., 123 cases in 2012]), an intestinal infection caused by the parasite cyclospora cayetanensis. by september 20, cdc had been notified of 643 cases from 25 states, primarily texas (278 cases), iowa (153), and nebraska (86). inves ... | 2013 | 24172881 |
| implications of culture-independent panel-based detection of cyclospora cayetanensis. | | 2013 | 23985921 |
| cystoisospora and cyclospora. | the present review focuses on recent epidemiological and diagnostic advances in cystoisosporiasis and cyclosporiasis. | 2013 | 23982239 |
| [drug therapy of infectious diarrhea. part 2: chronic diarrhea]. | diarrheal diseases are among the most common diseases worldwide. in this review the current treatment recommendations for acute (part 1) and chronic (part 2) infectious diarrhea are summarized and typical enteropathogens are discussed. the second part of the article describes chronic diarrhea, its related pathogens and treatment. in contrast to acute diarrhea which is mainly caused by viral and typical bacterial pathogens, chronic diarrhea has mainly non-infectious origins. protozoal pathogens, ... | 2013 | 23917963 |
| genetic characterization of human-pathogenic cyclospora cayetanensis parasites from three endemic regions at the 18s ribosomal rna locus. | cyclospora cayetanensis is an apicocomplexan parasite that infects the gastrointestinal tract and causes acute diarrheal disease in humans. in recent years, this human-pathogenic parasite has led to several foodborne outbreaks in the united states and canada, mostly associated with imported produce. understanding the biology and epidemiology of c. cayetanensis is difficult because little is known about its origin, possible zoonotic reservoirs, and genetic relationships with other coccidian paras ... | 2014 | 23891667 |
| co-infection with cyclospora cayetanensis and salmonella typhi in a patient with hiv infection and chronic diarrhoea. | a 45-year-old-haitian male patient with fever, abdominal cramping, chronic diarrhoea and weight loss of about 3 kg was investigated. stool examination revealed salmonella typhi and cyclospora cayetanensis. the hiv test was positive with a cd4 count of 130 cells/mm(3). we provided the first report of co-infection cyclospora cayetanensis and salmonella typhi in a hiv patient with chronic diarrhoea. the patient was treated with oral ciprofloxacin, 500 mg, twice daily for two weeks, with a good clin ... | 2013 | 23432863 |
| foodborne outbreaks in canada linked to produce: 2001 through 2009. | foodborne disease outbreaks associated with fresh fruits and vegetables have been increasing in occurrence worldwide. canada has one of the highest per capita consumption rates of fresh fruits and vegetables in the world. in this article, we review the foodborne disease outbreaks linked to produce consumption in canada from 2001 through 2009. the 27 produce-related outbreaks included an estimated 1,549 cases of illness. bacterial infection outbreaks represented 66% of the total. among these, sal ... | 2013 | 23317873 |
| [clinical and epidemiological study of intestinal coccidioses in a rural population of a semiarid region from falcon state, venezuela]. | although intestinal coccidioses caused by cryptosporidium sp., cystoisospora belli and cyclospora cayetanensis are parasitic diseases of major clinical importance in public health, several clinical and epidemiological aspects of these diseases still remain unknown in falcon state, venezuela. a cross-sectional survey was conducted between june and october 2011, to investigate clinical and epidemiological data in 157 people (x +/- s.d. = 21.28 +/- 17 years-old) from sabaneta, falcón state, venezue ... | 2012 | 23248971 |
| indigestion after digestive disease week (ddw): be aware of the "golden raspberry award" at ddw and cyclospora cayetanensis. | | 2012 | 23211863 |
| [research progress on the biology and epidemiology of cyclospora cayetanensis]. | cyclosporiasis is one of the emerging parasitic diseases. cyclospora cayetanensis is so far the only species infecting humans in the cyclospora genus. this paper reviews mainly the biological characteristics of c. cayetanensis and the current epidemiology status of human infection. | 2012 | 23072169 |
| survey and genetic characterization of wastewater in tunisia for cryptosporidium spp., giardia duodenalis, enterocytozoon bieneusi, cyclospora cayetanensis and eimeria spp. | the microbial diversity of wastewater used for irrigation and fertilization was assessed using specific polymerase chain reaction (pcr) assays to detect and genotype several pathogenic protists including cryptosporidium spp., giardia duodenalis, cyclospora spp., eimeria spp. and enterocytozoon bieneusi. a total of 220 wastewater samples (110 raw, 110 treated) and 12 sludge samples were collected from 2005 to 2008 from 18 treatment plants located throughout tunisia. except for cyclospora, which w ... | 2012 | 22960487 |
| sequence characterization of heat shock protein gene of cyclospora cayetanensis isolates from nepal, mexico, and peru. | we have described the development of a 2-step nested pcr protocol based on the characterization of the 70-kda heat shock protein (hsp70) gene for rapid detection of the human-pathogenic cyclospora cayetanensis parasite. we tested and validated these newly designed primer sets by pcr amplification followed by nucleotide sequencing of pcr-amplified hsp70 fragments belonging to 16 human c. cayetanensis isolates from 3 different endemic regions that include nepal, mexico, and peru. no genetic polymo ... | 2013 | 22924935 |
| hiv/aids-associated opportunistic protozoal diarrhea. | human immunodeficiency virus (hiv) infection has altered both the epidemiology and outcome of enteric opportunistic parasitic infections. this study was done to determine the prevalence and species/genotypes of intestinal coccidian and microsporidial infections among hiv/aids patients with diarrhea and/or a history of diarrhea alternately with an asymptomatic interval, and their association with cd4 t cell count. this cross-sectional study was done from may 2010 to may 2011 in shiraz university ... | 2013 | 22873400 |
| what a professor learned about cyclospora cayetanensis by attending digestive diseases week conference in chicago. | | 2012 | 22764034 |
| first detection of acalculous cholecystitis associated with sarcocystis infection in a patient with aids. | acalculous cholecystitis and cholangitis are increasingly being recognized as complications of aids. the opportunistic parasites that have been most commonly associated with these disorders are cryptosporidium species, isospora belli, cyclospora cayetanensis and enterocytozoon bieneusi. the authors performed a parasitological survey on the gallbladder tissue sections of patients underwent cholecystectomy due to chronic acalculous cholecystitis at the shiraz university of medical sciences, iran. ... | 2014 | 24827104 |
| occurrence of cryptosporidium, giardia, and cyclospora in influent and effluent water at wastewater treatment plants in arizona. | we investigated the occurrence of cryptosporidium, giardia, and cyclospora at two wastewater treatment plants (wwtps) in arizona over a 12-month period, from august 2011 to july 2012. influent and effluent wastewater samples were collected monthly, and protozoan (oo)cysts were concentrated using an electronegative filter, followed by the detection of protozoa using fluorescent microscopy (cryptosporidium oocysts and giardia cysts) and pcr-based methods (cryptosporidium spp., giardia intestinalis ... | 2014 | 24695096 |
| the prevalence of cyclospora cayetanensis and cryptosporidium spp. in turkish patients infected with hiv-1. | opportunistic infections such as cryptosporidiosis and cyclosporiasis are commonly encountered in patients with acquired immunodeficiency syndrome (aids). we investigated the existence of opportunistic protozoans that significantly affect the quality of life in hiv-1 infected patients using conventional and molecular methods. the study group comprised 115 hiv-1 positive patients. in the identification of cyclospora cayetanensis and cryptosporidium, the formol-ether precipitation method was used ... | 2017 | 28682770 |
| evaluation of an improved u.s. food and drug administration method for the detection of cyclospora cayetanensis in produce using real-time pcr. | cyclospora cayetanensis is a protozoan parasite that causes human diarrheal disease associated with the consumption of fresh produce or water contaminated with c. cayetanensis oocysts. in the united states, foodborne outbreaks of cyclosporiasis have been linked to various types of imported fresh produce, including cilantro and raspberries. an improved method was developed for identification of c. cayetanensis in produce at the u.s. food and drug administration. the method relies on a 0.1% alcono ... | 2017 | 28590822 |
| [cyclospora cayetanensis]. | | 2017 | 28394980 |
| detection and prevalence of protozoan parasites in ready-to-eat packaged salads on sale in italy. | to investigate the prevalence of protozoan contamination by giardia duodenalis, cryptosporidium spp., toxoplasma gondii and cyclospora cayetanensis, in 'ready to eat' (rte) salads on sale in italy, 648 packages were purchased from industrial and local brands. nine individual packages from each brand were collected per month, pooled and subjected to microscopy and molecular analyses. microscopic examination of 864 slides detected cryptosporidium spp. but also blastocystis hominis and dientamoeba ... | 2017 | 28648295 |
| simultaneous molecular detection of cryptosporidium and cyclospora from raw vegetables in korea. | cryptosporidium and cyclospora are well-known coccidian protozoa that can cause waterborne and foodborne diarrheal illnesses. there have been a few reports regarding contamination in different vegetables with cryptosporidium, but no data are available regarding the sources of cyclospora infections in korea. in the present study, we collected 6 kinds of vegetables (perilla leaves, winter-grown cabbages, chives, sprouts, blueberries, and cherry tomatoes) from july 2014 to june 2015, and investigat ... | 2017 | 28506035 |
| multilocus sequence typing and clonal population genetic structure of cyclospora cayetanensis in humans. | to investigate the prevalence of cyclospora cayetanensis in a longitudinal study and to conduct a population genetic analysis, fecal specimens from 6579 patients were collected during the cyclosporiasis - prevalent seasons in two urban areas of central china in 2011-2015. the overall incidence of c. cayetanensis infection was 1·2% (76/6579): 1·6% (50/3173) in zhengzhou and 0·8% (26/3406) in kaifeng (p 0·05). all the isolates clustered in the c. cayetanensis clade based on the small subunit ribos ... | 2017 | 28697813 |