| variations in the virulence of strains of chlamydia psittaci for pregnant ewes. | invasive and non-invasive strains of chlamydia psittaci isolated from faeces of clinically healthy ewes and from vaginal swabs of ewes which had aborted were injected intravenously or intradermally into pregnant ewes. the results were studied by recording the ewes' thermal and serological responses, lambing performance and the excretion of chlamydia from the vagina. the differences between the effects of different invasive strains were greater after intradermal inoculation than after intravenous ... | 1989 | 2773238 |
| [incidence of coxiella burnetti and chlamydia psittaci in cattle in southern bohemia]. | in 1986 and 1987 in six districts and 22 localities of the southern bohemian region, and in one district and one locality of the western bohemian region the total of 747 head of cattle was examined by a microagglutination method (mar) and complement fixation method (kfr) for antibodies to coxiella burnetii and chlamydia psittaci. the positivity of c. burnetii detection was 44.4% in mar and 16.5% in kfr. the positivity of chl. psittaci detection was 7.1%. the widespread incidence and highly posit ... | 1989 | 2800248 |
| seroprevalence of chlamydia psittaci-specific antibodies in small stock in namibia--epidemiological study with an enzyme-linked immunosorbent assay (elisa). | an igg (h+l)-elisa was applied as a screening test for antibodies against chlamydia (c.) psittaci in sera of goats and sheep in namibia. in 576 (27.3%) of a total of 2,107 sera (299 = 25.2% of 1,185 caprine and 277 = 30.0% of 922 ovine sera) chlamydial antibodies could be detected. 86% of all farms tested revealed seropositive animals. chlamydial infections were prevalent in all the geographical regions tested. the infection rates per state veterinary district varied from 12.0% (otjiwarongo) to ... | 1989 | 2800787 |
| characterization of the new chlamydia agent, twar, as a unique organism by restriction endonuclease analysis and dna-dna hybridization. | several molecular techniques were used for comparison of the novel chlamydia agent, twar, with chlamydia trachomatis and chlamydia psittaci. unlike all serotypes of c. trachomatis and most strains of c. psittaci, the eight twar isolates examined did not contain extrachromosomal dna. twar was readily distinguished from c. trachomatis or c. psittaci by restriction endonuclease analysis, whereas identical or nearly identical restriction patterns were observed among the twar isolates. southern blot ... | 1987 | 2822763 |
| restriction endonuclease analysis of dna from two isolates of chlamydia psittaci obtained from human abortions. | | 1987 | 2825905 |
| comparison of chlamydia psittaci isolates by restriction endonuclease and dna probe analyses. | dnas from eight chlamydia psittaci isolates (koala conjunctivitis, avian psittacosis, avian ornithosis, ovine abortion, ovine polyarthritis, sporadic bovine encephalomyelitis, and feline conjunctivitis) and one chlamydia trachomatis isolate (lymphogranuloma venereum) were compared by restriction endonuclease and dna probe analyses. digestion with hindiii yielded a series of discrete fragments which allowed the differentiation of most isolates. a gene probe, pfen207, which encodes the chlamydia-s ... | 1988 | 2826336 |
| acute myocarditis. serologic diagnosis, clinical findings and follow-up. | in a prospective study, 57 patients with a preliminary diagnosis of myocarditis were investigated. twenty-four patients were considered to have an acute myocarditis, 14 had a suspected myocarditis, while in 19 patients myocarditis was excluded. episodes of frequent supraventricular and/or ventricular extrasystoles during hospital stay were seen in 8/24 cases (33%) with myocarditis and in 1/19 cases (5%) without myocarditis. on follow-up 1 month later, no supraventricular extrasystoles were obser ... | 1988 | 2831699 |
| serological investigations for some bacterial and viral pathogens in fallow deer (cervus dama) and wild boar (sus scrofa) of the san rossore preserve, tuscany, italy. | sera of 43 fallow deer (cervus dama) of the san rossore preserve (tuscany, italy) were examined for antibodies against eight pathogens; one proved positive for brucella sp., 21 for listeria monocytogenes, 34 for chlamydia psittaci, three for coxiella burnetii, one for infectious bovine rhinotracheitis virus, 11 for parainfluenza-3 virus, 25 for bovine viral diarrhea virus and six for bovine respiratory syncytial virus. no age and sex difference in the positivity rates and titers was evidenced, w ... | 1988 | 2832622 |
| restriction endonuclease analysis of dna from chlamydia trachomatis biovars. | dna from a total of 60 chlamydia trachomatis isolates was examined by restriction endonuclease analysis. strains from all established biovars and serovars were tested. there was great diversity between the mouse biovar and the lymphogranuloma venereum (lgv) and trachoma biovars. the lgv and trachoma biovar isolates generated similar fragment patterns; however, distinct fragments appeared to be unique to both biovars, thus allowing differentiation of these two major groups. in most cases, strains ... | 1988 | 2835386 |
| [serological survey of feline leukemia virus infection and the outcome of antibody-positive cats]. | a serological survey was carried out to examine the presence of antibodies against feline leukemia virus (felv) and feline oncornavirus-associated cell membrane antigen (focma) in 208 cat sera collected at teikyo university school of medicine. seven cats (3.4%) were positive for felv antibodies by enzyme-linked immunosorbent assay whereas no cat was positive for focma antibody by indirect membrane immunofluorescent test. anemia, leukemia and/or lymphoma formation were not observed in these felv ... | 1988 | 2840305 |
| [chlamydia twar respiratory infection in cytomegalovirus infection]. | a middle-aged male smoker with cytomegalovirus infection developed a respiratory tract infection, mostly bronchitis, associated with a low chlamydia psittaci titer and a high c. trachomatis titer in indirect immunofluorescence tests. this situation, previously encountered in children and elderly people having no contact with birds and no genital infection, was reminiscent of the twar strains interhuman infections described by grayston and wang. a serum sample was therefore sent to proff. graysto ... | 1988 | 2847139 |
| comparison of dna-spot hybridization, cell culture and direct immunofluorescence staining for the diagnosis of avian chlamydiae. | dna-spot hybridization, cell culture and direct immunofluorescence staining were compared for the detection of avian chlamydia psittaci strains in cell culture dilutions and in routine samples submitted for diagnosis. with dilutions of infected cell culture material, growth in bgm cells was by far the most sensitive technique, detecting 0.01 infected cells (20 elementary bodies) ml-1. dna-spot hybridization and direct immunofluorescence staining were of approximately equal sensitivity, both dete ... | 1988 | 2847401 |
| disulfide-mediated interactions of the chlamydial major outer membrane protein: role in the differentiation of chlamydiae? | the effects of exogenous reducing agents on a number of biological properties of purified chlamydia trachomatis lgv-434 and chlamydia psittaci meningopneumonitis elementary bodies (ebs) have been examined in an attempt to identify in vitro correlates of early events in the differentiation of the infectious eb to the replicative cell type, the reticulate body (rb). treatment of ebs with dithiothreitol elicited a number of changes normally associated with differentiation to the rb. ebs in the pres ... | 1985 | 2857160 |
| prevalence of various enteropathogens in the feces of diarrheic and healthy calves. | the presence of various enteropathogens was examined in the feces of homebred dairy calves reared in a restricted geographical area of france (north west of county of indre-et-loire) during winter 1983-1984. two distinct surveys were carried on: a case-control study including 32 diarrheic calves and 21 healthy calves bred in 53 different farms; and a separate study on nine diarrheic calves in another farm. the following infectious agents were looked for, by specific methods of detection: escheri ... | 1986 | 2877609 |
| exposure to chlamydia psittaci in pregnancy. | | 1987 | 2883467 |
| symptomatic chlamydia psittaci reinfection. | | 1988 | 2896822 |
| family outbreaks of psittacosis in israel. | eight family outbreaks of clinical or subclinical psittacosis in israel after exposure to infected birds were studied. throat cultures for chlamydia psittaci and serological tests for chlamydia species, including strain twar, were obtained from 37 people. cloacal smears and cultures of internal organs for c psittaci were taken from 9 dead birds. 62% of the people studied had symptoms, and 67% of the birds that died had previously been sick. evidence for acute c psittaci infection was found in 81 ... | 1988 | 2900989 |
| isolation of a gene encoding a chlamydia sp. strain twar protein that is recognized during infection of humans. | chlamydia sp. strain twar is a unique chlamydia sp. that causes acute respiratory disease. a gene bank consisting of twar isolate ar-39 dna in puc19 was screened with anti-ar-39 rabbit immune sera. two positive clones were isolated that contained 7.3-kilobase (plc1) and 14.9-kilobase (plc2) plasmids. restriction mapping and hybridization studies showed that both plc1 and plc2 contained a common 4.2-kilobase psti fragment. plasmids were used as templates of in vitro transcription-translation. all ... | 1989 | 2909493 |
| superimposed high frequency ventilation with conventional mechanical ventilation. | a 73-year-old man with ards-multiple organ failure due to chlamydia psittaci, was successively supported with conventional respiratory techniques. after 48 hours of no clinical improvement, hfv was superimposed to cmv in order to combine the advantages of each one. since improvement has been seen in all ventilatory parameters, this method is suggested as another mode of ventilation for patients with refractory hypoxia and hypercarbia who do not respond to conventional respiratory care. | 1989 | 2920598 |
| mouse models for evaluation of virulence of chlamydia psittaci isolated from ruminants. | intestinal strains of chlamydia psittaci isolated from faeces of clinically healthy sheep or goats were differentiated from pathogenic strains isolated from animals affected with chlamydial diseases by their virulence in mice. the invasiveness of strains after footpad inoculation was estimated in non-pregnant mice by recording the colonisation and the enlargement of the spleen on days 6 and 9 after inoculation. the abortifacient effect was judged by measuring the colonisation of placenta and fet ... | 1989 | 2922503 |
| comparison of protein patterns between invasive and non-invasive ovine strains of chlamydia psittaci. | protein patterns displayed in sodium dodecyl sulphate (sds) electrophoresis by invasive and noninvasive strains of chlamydia psittaci showed three constant differences, the most distinctive being a band at 90 kd from invasive strains. in comparison with other methods so far described, this method provides a more efficient means of differentiating between invasive and non-invasive strains. furthermore, it may lead to the development of improved methods of diagnosis. | 1989 | 2922504 |
| relationship between virulence of chlamydia psittaci strains and establishment of persistent infection of mccoy cells. | the pathogenicity of chlamydial strains for their natural hosts and their ability to induce persistent infections in mccoy cells were compared. both virulent and avirulent strains persistently infected mccoy cells, but the appearance of the cell culture varied between strains. avirulent strains induced completely inapparent persistent infection (infection type 1), while with invasive strains the culture alternated between periods of cell multiplication and periods of extensive cytopathic change ... | 1989 | 2922922 |
| chlamydial pelvic infection in cats: a model for the study of human pelvic inflammatory disease. | the inoculation of feline keratoconjunctivitis agent (chlamydia psittaci) directly into the oviducts of eight cats produced an acute disease that was characterised by hyperaemia of the tissue and pronounced polymorphonuclear leucocyte infiltration of the epithelium and subepithelial stroma. the lumens of the tubes contained exudates of desquamated epithelial cells and polymorphonuclear leucocytes. after about 30 days the disease subsided leaving chronic inflammation with the tissue infiltrated w ... | 1985 | 2931347 |
| [etiological structure of pneumonias in children and adults]. | the bacteriological study of sputa, nasopharyngeal smears and bronchial washings taken from pneumonia patients has shown that the leading etiological agent was streptococcus pneumoniae isolated in the diagnostic titre (10(7) bacteria per ml) in 78.1% of the cases. staphylococcus aureus, haemophilus influenzae, enterobacteria and yeast-like fungi have been found to play an insignificant role in the etiology of acute pneumonia (2.5 +/- +/- 0.9%). the results of the serological diagnosis by means o ... | 1987 | 2960106 |
| antigens of chlamydia trachomatis. | chlamydia trachomatis is an obligate intracellular parasite that elaborates antigens on its surface. these antigens are divided into genus-, species-, subspecies-, and serovar-specific determinants. the genus, or group antigen(s), are lipopolysaccharides similar to those found in gram-negative bacteria and a glycolipid that is secreted by infected cell cultures. species-specific antigens differentiate chlamydia trachomatis from chlamydia psittaci and are expressed on the outer membrane. these pr ... | 1985 | 2999944 |
| monoclonal antibodies against chlamydia psittaci. | five monoclonal antibodies were prepared against chlamydia (c.) psittaci strain pigeon-1041 isolated from a feral pigeon in sapporo. reactions of these antibodies to chlamydiae were examined using five strains of c. psittaci and two strains of c. trachomatis in an enzyme-linked immunosorbent assay, microimmunofluorescent test and complement fixation test. the antibodies were divided into two groups: three genus-specific (a2, d2, and i21) and two strain-specific (f2 and h9) antibodies. the antige ... | 1986 | 3025566 |
| mixed infection with chlamydia psittaci, fowlpox virus and haemophilus gallinarum in broiler breeder chicks. | | 1987 | 3037759 |
| pneumonias in adults due to mycoplasma, chlamydiae, and viruses. | pneumonias in adults due to mycoplasma, chlamydiae, and viruses are a common clinical problem. these microorganisms contribute to the etiologies in 6-35% of all cases of pneumonia and are the sole pathogens in 1-17% of hospitalized cases. important trends and developments in the field include the emergence of a chlamydia psittaci strain (twar) that is passaged from human to human, causes a mycoplasma-like illness, and that is relatively resistant to erythromycin, the recognition of respiratory s ... | 1987 | 3037901 |
| analysis and preparation of bartonella bacilliformis antigens. | twenty-four antigens of bartonella bacilliformis, a bacterium which causes bartonellosis in residents of high altitude valleys of the andes, were identified by immunoblot and immunoprecipitation using rabbit anti-bartonella sera as well as sera of patients. the antigens were designated according to their relative molecular mass which ranged from 16 to 160 kda. twelve antigens were detected by antibodies in sera of bartonellosis patients using immunoblot, of which six antigens were detected by im ... | 1988 | 3044156 |
| [method of titration of chlamydia psittaci by immunofluorescence]. | | 1988 | 3049616 |
| chlamydial antibodies in farmers in north-west england. | because of recent reports of abortion in farmers' wives following infection with ovine strains of chlamydia psittaci during pregnancy, the distribution of chlamydial antibodies was studied in rural populations in north-west england, where endemic chlamydial infection with abortion is common in sheep. immunoperoxidase assays with c. trachomatis and ovine c. psittaci showed no significant differences in either the frequency or titres of antibodies between sheep farmers and other types of farmer or ... | 1988 | 3053222 |
| shedding and transmission of chlamydia psittaci in experimentally infected chickens. | three avian strains of chlamydia psittaci were inoculated into 8-day-old chickens by the intra-air-sac or peroral route. uninoculated chickens were kept as cagemates with the air-sac-inoculated birds. the air-sac-inoculated birds had systemic infection, and chlamydiae were detected frequently in the livers, lungs, jejunums, and colo-rectums at high titers (greater than or equal to 10(5.0) eld50). all three groups of birds had intermittent and persistent shedding of chlamydiae into feces during t ... | 1988 | 3060085 |
| molecular cloning and sequence analysis of a developmentally regulated cysteine-rich outer membrane protein from chlamydia trachomatis. | two overlapping genomic fragments have been cloned from chlamydia trachomatis serovar l1 dna. sequence determination of 2530 bp has revealed two open reading frames coding for 'cysteine-rich' (cr) proteins. one of these proteins was confirmed, by analysis of the inferred amino acid sequence, as the 60-kda cr outer membrane protein associated with differentiation of reticulate bodies (rbs) into elementary bodies (ebs). the other smaller 15-kda protein contained a high percentage of methionine and ... | 1988 | 3066701 |
| oxygen-independent inhibition of intracellular chlamydia psittaci growth by human monocytes and interferon-gamma-activated macrophages. | we have demonstrated previously that chlamydia psittaci grows well in human monocyte-derived macrophages, but to a limited extent in lymphokine-or interferon-gamma (ifn-gamma)-activated macrophages. in this investigation, freshly explanted human monocytes inhibited chlamydial inclusion formation by 85% as compared to macrophages, and the level of inhibition was similar to that exhibited by lymphokine-activated macrophages (79%). to determine whether the oxygen-dependent antimicrobial mechanisms ... | 1986 | 3088106 |
| induction of tryptophan catabolism is the mechanism for gamma-interferon-mediated inhibition of intracellular chlamydia psittaci replication in t24 cells. | human uroepithelial (t24) cells were incubated for 24 h in the presence of various concentrations of human recombinant gamma interferon (hu-rifn-gamma) and then infected with the 6bc strain of chlamydia psittaci. this resulted in a reduction of intracellular chlamydial inclusion development in proportion to the concentration of hu-rifn-gamma present when giemsa-stained cells were examined by light microscopy 24 h after infection. when tryptophan was added to hu-rifn-gamma-treated cells just afte ... | 1986 | 3089936 |
| [evaluation of sero-diagnostic technics in the diagnosis of chlamydia psittaci infection, a study on experimental infection]. | | 1986 | 3093599 |
| identification of a new group of chlamydia psittaci strains called twar. | a new group of chlamydia psittaci strains has been identified. they are called twar after the laboratory designation of the first two isolates. twelve strains were isolated from pharyngeal swabs of different persons with acute respiratory disease in seattle, wash., during 1983 to 1986. one strain was obtained from the eye of a child during the trachoma vaccine study in taiwan in 1965. nine strains were characterized in this study. twar organisms formed intracytoplasmic inclusions in hela cells w ... | 1986 | 3097063 |
| [a case of pneumonia associated with increased antibody titers to simultaneous mycoplasma pneumoniae and chlamydia psittaci]. | | 1986 | 3100694 |
| [study of the reactivity and immunogenicity of a combined vaccine against abortion due to coxiellosis and chlamydiosis in sheep]. | reactogenicity was studied in a combined vaccine against q-fever and chlamydia-induced abortion in ewes; the vaccine consisted of a mixture of purified corpuscles of chlamydia psittaci (100 micrograms) and coxiella burnetii (50 micrograms). the immunogenicity of the combined vaccine, evaluated by the number of positive serums after vaccination and by the average geometric titre of antibodies, was the same as that of the vaccine separate constituents as to the antibodies to c. burnetii, but somew ... | 1987 | 3111068 |
| [demonstration of antibodies against chlamydia psittaci and coxiella burnetii in dogs and cats: comparison of the enzyme immunoassay, immunoperoxidase technic, complement fixation test and agar gel precipitation test]. | | 1987 | 3113133 |
| in vivo-activated mononuclear phagocytes and protective immunity to chlamydiae in mice. | peritoneal macrophages (m phi s) collected from chlamydia psittaci 6bc-immune mice after intraperitoneal challenge with 10(6) 6bc (immune-boosted [ib] m phi s) were compared by various functional criteria with other in vivo- and in vitro-activated m phi populations. while casein-, protease peptone-, and thioglycolate (thio)-elicited m phi s were equally susceptible to in vitro infection with 6bc, ib m phi s did not support chlamydial growth and m phi s from mycobacterium tuberculosis bcg- or lis ... | 1988 | 3131246 |
| demonstration of chlamydia psittaci antigen in smears and paraffin tissue sections using a fluorescein isothiocyanate labelled monoclonal antibody. | | 1988 | 3135791 |
| [confusional form of chlamydia psittaci encephalitis. diagnostic value of microimmunofluorescence. a case]. | a rare case of chlamydia psittaci encephalitis is reported. the disease started with anxiety, agitation and fever (38.5 degrees c) accompanied with hallucinations and regressed within 48 hours, but a confusional syndrome persisted for 9 days. alterations in the blood-brain barrier with low csf protein levels and signs of lateralization could be demonstrated. the inflammatory syndrome remained discrete. the cause of the disease was disclosed by serology and epidemiological investigations. the spo ... | 1985 | 3156331 |
| [a study of antichlamydial effect of rokitamycin]. | mics of a new macrolide antibiotic, rokitamycin (rkm), for chlamydia psittaci and chlamydia trachomatis were determined. meanwhile, the organisms were observed under the electron microscope for morphologic changes with the addition of rkm. 1. mics of rkm for c. psittaci mp and 3 strains of c. psittaci isolated from budgerigars kept by patients ranged from 0.05 to 0.10 microgram/ml, and those for 3 strains of c. trachomatis b, e and l2 ranged from 0.20 to 0.39 microgram/ml. these mics were higher ... | 1988 | 3172456 |
| isolation and serological survey of chlamydia psittaci in sheep in hokkaido. | | 1988 | 3172598 |
| anti-chlamydial immunity in ewes conferred by vaccination with a combination of three live chlamydia, brucella and salmonella vaccines. | live attenuated vaccines against chlamydia psittaci var ovis, brucella melitensis and salmonella abortus ovis have previously been shown to be compatible in mice by subcutaneous administration. immunity against challenge with virulent chlamydia was, however, slightly decreased in associations including the b melitensis rev 1 vaccine. the chlamydia strain 1b vaccine was administered to four- to five-month-old female lambs, either alone or in combination with the b melitensis rev1 and the s abortu ... | 1988 | 3176252 |
| protein synthesis early in the developmental cycle of chlamydia psittaci. | the incorporation of [35s]methionine into protein by intracellular and host-free chlamydia psittaci 6bc was analyzed at intervals between 15 min and 28 h postinfection by autoradiography of sodium dodecyl sulfate-polyacrylamide gels. the profiles of proteins synthesized in the two systems were similar at all times, indicating that the host-free system can be used to monitor the temporal expression of genes in chlamydiae. the host-free system permitted detection of synthesis of chlamydial protein ... | 1988 | 3182069 |
| distribution of plasmid sequences in avian and mammalian strains of chlamydia psittaci. | plasmid dna from an avian strain of chlamydia psittaci was purified and estimated to be 7.9 kb in size using restriction endonuclease analysis. a 5.9 kb fragment of this plasmid was cloned, mapped and used to screen a range of chlamydial strains. hybridizing dna was absent from ovine abortion and arthritis isolates and also from the cal 10 strain but related sequences were detected in c. psittaci strains of feline pneumonitis, guinea-pig inclusion conjunctivitis, ovine conjunctivitis and c. trac ... | 1988 | 3183616 |
| isolation and typing of a strain of chlamydia psittaci from angora goats. | | 1988 | 3190599 |
| isolation of chlamydia psittaci from the genital tract of lambs: a possible link with enzootic abortion of ewes. | | 1988 | 3195039 |
| relationship between infectivity and cytopathology for l-929 cells, membrane proteins, and antigenicity of avian isolates of chlamydia psittaci. | seventeen isolates of chlamydia psittaci from various avian species were examined. based on their infectivity and cytopathology for l-929 cells, these isolates were separable into a high-infectivity group (hig) and a low-infectivity group (lig). differences in the molecular weight of the major outer membrane proteins (momps) of the isolates correlated with differences in infectivity. the hig momps had a molecular weight of 43,500, and the lig momps had a molecular weight of 45,500. the momp of o ... | 1988 | 3196260 |
| a chicken model of systemic infection with chlamydia psittaci: comparison of the virulence among avian and mammalian strains. | | 1988 | 3210475 |
| monoclonal antibodies to chlamydia psittaci: characteristics and antigenic analysis. | | 1988 | 3290562 |
| immunotyping of chlamydia psittaci by indirect immunofluorescence antibody test with monoclonal antibodies. | monoclonal antibodies against pigeon and budgerigar strains of chlamydia psittaci were used to classify the immunotypes of c. psittaci strains by an indirect immunofluorescence antibody (ifa) test. thirty-three c. psittaci strains from pigeons and 24 from budgerigars were divided into three immunotypes (p-i, p-ii, and p-iii) and (b-i, b-ii, and b-iii), respectively. two strains from human psittacosis patients were identified as p-iii and b-i, coinciding with the epidemiological evidence of each ... | 1988 | 3292879 |
| production and partial characterization of monoclonal antibodies to four chlamydia psittaci isolates. | monoclonal antibodies (mabs) were produced to four chlamydia psittaci isolates: nj1 and tt3 from turkeys, vs1 from a parakeet, and b577 from an ovine abortion. mabs were tested for reactivity with each isolate by the indirect immunofluorescent antibody technique and for neutralization by an inclusion reduction neutralization technique in tissue culture. two genus-specific and 14 serovar-specific mabs were produced. genus-specific mabs reacted with all avian and mammalian isolates; however, each ... | 1988 | 3294184 |
| [comparative studies on the demonstration of antigens of chlamydia psittaci from deep organ samples using mouse assays and elisa (preliminary report)]. | | 1987 | 3297602 |
| [ofloxacin (ru 43280). clinical study]. | thirty-two patients were treated by ofloxacin on bacteriological documented infections. they were enterobacterias: n = 15 (mic less than or equal to 0.06 to 0.5 microgram/ml); pseudomonas aeruginosa and acinetobacter: n = 1 (mic 0.5 and 4 micrograms/ml); staphylococcus: n = 6 (mic less than or equal to 0.06 to 4 micrograms/ml); pneumococcus: n = 1; mycoplasma: n = 1; chlamydia psittaci: n = 2; legionella pneumophila: n = 1; rickettsias: n = 4 (three mediterranean fevers one query fever). ofloxac ... | 1987 | 3302873 |
| [comparative studies on the occurrence of chlamydia psittaci in organ samples of birds using the mouse infection test and elisa]. | | 1987 | 3304947 |
| detection of chlamydial inclusions in cell culture or biopsy tissue by alkaline phosphatase-anti-alkaline phosphatase staining. | an immunological technique for detecting chlamydia trachomatis and chlamydia psittaci inclusions in infected mccoy cell cultures was developed by using a genus-specific monoclonal antibody to chlamydia spp., rabbit anti-mouse immunoglobulin g bridging antibody, alkaline phosphatase-anti-alkaline phosphatase (apaap) monoclonal antibody conjugate, and naphthol as-phosphate/fast red substrate. chlamydial inclusions stained red and were easily detected against a background of blue hematoxylin-staine ... | 1987 | 3312286 |
| q fever vaccines for animals. | the results of different workgroups and those obtained in own investigations with q fever vaccines for cattle and sheep are reviewed and discussed. in field trials coxiella (c.) burnetii vaccines of inactivated whole cells (wc) in phase (ph) i as well as wc ph ii vaccines prevented infections of cows exposed to naturally infected environments, provided they were vaccinated as noninfected calves. to monitor calves for this purpose the sensitive elisa has superseded the complement fixation test (c ... | 1987 | 3324574 |
| [epidemiologic significance of the immunofluorescence typing of chlamydia psittaci]. | a group of 39 strains isolated from pigeons, parakeets, parrots, sheep, goats, cats, guinea-pigs, mice and humans were immunotyped by a one-way or two-way cross-reaction micro-immunofluorescence test. eight immunotypes were found. among them, two new immunotypes were characterized: a cat conjunctivitis strain and a mouse inapparent respiratory infection strain. the immunotypes of feline pneumonitis and guinea-pig conjunctivitis, recently described by perez-martinez and storz, were confirmed. thr ... | 1986 | 3324944 |
| chlamydia parasitism: ultrastructural characterization of the interaction between the chlamydial cell envelope and the host cell. | ultrastructural analysis of the growth cycles of chlamydia trachomatis and chlamydia psittaci showed that the chlamydial cell envelope became rigid and septated at the time of the reorganization from reticulate to elementary body. this process occurred in the immediacy of the inclusion membrane and in close proximity with the mitochondria or the endoplasmic reticulum of the host cell. | 1988 | 3343223 |
| immunochemical diversity of the major outer membrane protein of avian and mammalian chlamydia psittaci. | immunochemical properties of the major outer membrane protein (momp) of 16 strains of chlamydia psittaci isolated from psittacine birds, budgerigars, a pigeon, turkeys, humans, cats, a muskrat, sheep, and cattle and a strain of c. trachomatis, l2/434/bu, were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by immunoblotting analysis with hyperimmunized rabbit antisera to strains of parrot, turkey, feline, and bovine origin. the momps of the strains showed variation in m ... | 1988 | 3366861 |
| evaluation of an enzyme immunoassay test for the diagnosis of chlamydia psittaci infection in free-ranging koalas (phascolarctos cinereus) in southeastern queensland, australia. | the ideia chlamydia test, a commercially available antigen-capture enzyme-linked immunosorbent assay (elisa) test, based on a monoclonal antibody for the detection of chlamydia in clinical specimens, was evaluated in a population of 65 free-ranging koalas in southeastern queensland determined to be infected with chlamydia psittaci. compared to isolation of the organism in tissue culture, the sensitivity of the ideia test ranged from 3 to 11%, and the specificity from 90 to 97%. the results indic ... | 1988 | 3373632 |
| aspects of the epidemiology of chlamydia psittaci infection in a population of koalas (phascolarctos cinereus) in southeastern queensland, australia. | a population of free-ranging koalas in southeastern queensland was examined to determine the prevalence of chlamydia psittaci infections. although c. psittaci was isolated from 46 of 65 (71%) koalas studied, only six (9%) of these had clinical signs of disease. most adult females (82%) had back or pouch young present even though 67% of them were infected. there were no significant correlations between age, sex or site of sampling (urogenital versus conjunctival tissues) and the isolation of c. p ... | 1988 | 3373633 |
| chlamydia psittaci: is tonsillar tissue the portal of entry in ovine enzootic abortion? | ewes at 70 days of gestation were exposed by various routes to a culture of chlamydia psittaci. chlamydial infection of fetal tissues, generally accompanied by abortion, was observed only in four of six ewes inoculated via the tonsillar crypts and in five of seven ewes injected subcutaneously; most of these also developed antibodies to c psittaci. seroconversion after normal lambing was also observed in most ewes inoculated orally or by stomach tube, despite failure to detect chlamydia in them. | 1988 | 3387681 |
| [detection of chlamydia psittaci in the feces of swine]. | | 1988 | 3390133 |
| two distinct forms of chlamydia psittaci associated with disease and infertility in phascolarctos cinereus (koala). | while several diseases associated with chlamydia psittaci infection have been reported in phascolarctos cinereus (koala), it is still unclear whether one or more chlamydial strains are responsible. in this study, we provide evidence, obtained by restriction enzyme and gene probe analysis, that two quite distinct strains of c. psittaci infect koalas; one strain was isolated from the conjunctivae, and the other was isolated from the urogenital tract and the rectum. a gene probe, pfen207, containin ... | 1988 | 3397180 |
| [the occurrence of chlamydia psittaci in the genital tract and feces of slaughtered female cattle]. | | 1988 | 3420992 |
| specific interaction of bovine igg1 and igg2 subclasses with different chlamydial antigens. | antigens of the immunotype 1 strain b-577 of chlamydia psittaci, which were separated by sds-page and electrophoretically transferred to nitrocellulose membranes were used to probe sequential serum samples of cattle with experimentally induced or naturally occurring chlamydial infections. applying igg1- and igg2-specific markers in an enzyme immunoassay procedure, a predominance of igg2 reactions with different proteins was determined. the interaction of igg1 with antigens such as the genus-spec ... | 1987 | 3425033 |
| [the occurrence of latent intestinal chlamydia psittaci infection in cattle]. | | 1987 | 3426536 |
| detection of chlamydia psittaci from various animal species by reverse passive haemagglutination. | a reverse passive haemagglutination test (rph) has previously been developed to detect the genus-specific antigen of chlamydia. clinical samples were obtained from various sites of different animal species. the rph test detected chlamydial antigen from clinical cases of conjunctivitis in cats, abortion in sheep and psittacosis in birds. although not as sensitive as cell culture isolation, this test has the advantages of rapidity and of detecting antigen from dead chlamydiae. | 1987 | 3439015 |
| [the epizootic process in ewe lambs experimentally infected with salmonella abortus ovis and chlamydia psittaci var. ovis]. | studied was the dynamics of antibody production and salmonella shedding in a flock of year-old female sheep affected with a mixed form of infection of salmonella abortus ovis and chlamydia psittaci var. ovis. it was established that in the presence of a mixed infection dominating was the part played by chlamydia organisms. the apparent epizootic process with these agents usually ran its course in the later period of pregnancy, while salmonella organisms acted by the end of the first half of preg ... | 1987 | 3439023 |
| the effect of intracellular chlamydia psittaci on lysosomal enzyme activities in mouse peritoneal macrophages. | live or heat killed (30 min at 56 degrees c) chlamydia psittaci elementary bodies (eb) were phagocytosed by mouse peritoneal macrophages. inoculation with killed chlamydia caused rises in three lysosomal enzyme activities tested, especially in acid phosphatase activity. in contrast, after infection with live chlamydia, only a negligible increase was seen in acid phosphatase activity, and no change in the activities of beta-glucuronidase and cathepsin d was observed. it was concluded that regulat ... | 1987 | 3442238 |
| [vaccination against bovine chlamydial abortion with a temperature-sensitive mutant of chlamydia psittaci]. | the safety of the vaccinal strain 1b was studied on cows during the pregnancy following the vaccination by recording complement fixing (cf) antibody titer, chlamydial excretion and calving performance. the immunity of vaccinated cows was challenged 19 months after vaccination by intradermal inoculation of 10(7) pfu of the virulent strain av1 during the 7th month of pregnancy. | 1987 | 3451688 |
| antichlamydial activity of ofloxacin. | the in vitro activity of ofloxacin, a new pyridone-carboxylic acid, against 11 strains of chlamydia trachomatis and six strains of chlamydia psittaci was determined. all test strains of both species were inhibited by 0.39 micrograms of ofloxacin per ml. the antichlamydial activity of ofloxacin was comparable to that of doxycycline and four- to eightfold less than that of minocycline. the results of this susceptibility test, coupled with those of previous pharmacokinetic analyses of ofloxacin, wa ... | 1986 | 3462450 |
| ocular delayed hypersensitivity: a pathogenetic mechanism of chlamydial-conjunctivitis in guinea pigs. | we used a naturally occurring, chlamydia psittaci-caused eye disease in guinea pigs, guinea pig inclusion conjunctivitis, as an animal model to understand both the immune response and the pathogenesis of chlamydial eye infections. when instilled into the conjunctival sac of guinea pigs that had been previously infected and were immune, viable chlamydiae or a triton x-100-soluble extract of them produced a short-lived (12-48 hr) eye disease indistinguishable clinically and histologically from tha ... | 1986 | 3463978 |
| t cell mediated and humoral immunity in a mouse chlamydia psittaci systemic infection. | immunity against chlamydia psittaci, an obligate intracellular parasite, was studied in a mouse model of systemic infection. sera (0.1 ml) and splenic cells (2 x 10(8)) from immunised mice were given intravenously to susceptible mice 16 hours before intravenous challenge with 1 x 10(5) plaque forming units (pfu) of virulent strain ab7. transfer of immune cells primed with virulent strain ab7 or vaccinal strain 1b, lowered splenic and hepatic colonisation by approximately 5.5 log pfu. treatment o ... | 1987 | 3498194 |
| in-vitro activity of roxithromycin against respiratory and skin pathogens. | the activity of roxithromycin was determined by a microdilution method, in comparison with erythromycin, spiramycin and josamycin. roxithromycin and erythromycin showed very similar mics against staphylococci, streptococcus pneumoniae, str. pyogenes and haemophilus influenzae. in most cases, spiramycin and josamycin appeared similarly or more active. the activity of roxithromycin against mycoplasma pneumoniae, legionella spp., chlamydia psittaci and, to some extent, against pasteurella spp. was ... | 1987 | 3501424 |
| an enzyme-linked immunosorbent assay (elisa) for anti-chlamydial secretory immunoglobulin a in guinea pig tears. | a method is described which permits the assay of specific secretory immunoglobulin a (siga) antibodies produced by guinea pigs in response to ocular infection with the guinea pig inclusion conjunctivitis strain of chlamydia psittaci (gpic agent). the enzyme-linked immunosorbent assay (elisa) developed was shown to be more sensitive and less subjective than the micro-immunofluorescence assay as a means of assaying specific antibody. | 1986 | 3511151 |
| [keratoconjunctivitis caused by chlamydia psittaci]. | a 25-year-old female patient suffering from acute follicular keratoconjunctivitis with epithelial and subepithelial corneal infiltrates was examined. antibodies of the igm class directed against c. psittaci were found. infection due to c. trachomatis was ruled out by serology (elisa) and negative isolation attempts. the patient's cat had rhinitis and conjunctivitis and a positive c. psittaci specific igm antibody titer. systemic application of tetracycline resulted in fast resolution of symptoms ... | 1986 | 3515033 |
| potential danger to pregnant women of chlamydia psittaci from sheep. | | 1986 | 3521058 |
| etiologic significance of bacterial isolates from rams with palpable epididymitis. | identified and partly identified bacterial isolates were obtained from 48 rams of various breeds that had unilateral or bilateral epididymitis. most of the animals were approximately 1 year of age; a few were older. brucella ovis, actinobacillus spp, corynebacterium spp, haemophilus spp, acinetobacter spp, escherichia coli, moraxella spp, staphylococcus spp, pasteurella spp, streptococcus spp, and chlamydia psittaci were isolated. a vaccine strain of b ovis, isolated species of bacteria, and mix ... | 1986 | 3521407 |
| bovine chlamydial abortion: serodiagnosis by modified complement-fixation and indirect inclusion fluorescence tests and enzyme-linked immunosorbent assay. | sequential serum samples from 11 cows experimentally inoculated with different abortigenic strains of chlamydia psittaci were tested by a modified complement-fixation (mocf) test, an indirect inclusion fluorescence antibody (iifa) test, and an enzyme-linked immunosorbent assay (elisa). one of these cows was not pregnant, another gave birth at term to a healthy calf, and all the others prematurely delivered infected dead calves or weak live calves. the results achieved with these tests on sera of ... | 1986 | 3527000 |
| prevalence of legionnaires' disease in pneumonia patients admitted to a danish department of infectious diseases. | during a 14-month study period, 92 patients admitted to the university clinic for infectious diseases with pneumonia were investigated to determine the prevalence and severity of legionnaires' disease (ld). the diagnosis of ld was based on positive serology. antibodies to 10 different legionella antigens--legionella pneumophila serogroups 1-6, fluoribacter (legionella) bozemanae, f. dumoffii, f. gormanii, and tatlockia (legionella) micdadei--were measured by the microagglutination (ma) and indir ... | 1986 | 3532303 |
| propagation of ovine and bovine abortion strains of chlamydia psittaci in suspension cultures of l-cells. | | 1986 | 3535313 |
| elisa for detection of human antibodies to chlamydiae. | an enzyme-linked immunosorbent assay for the detection of human antibodies to chlamydiae is described which exploits the cross-react properties between the genus-specific antigen of chlamydiae and the relps constituent of the outer membrane of a salmonella minnesota mutant. of 100 random sera tested by elisa-relps and immunofluorescence 78% showed an absolute correlation, 15% were positive in immunofluorescence and negative in elisa and 7% were positive in elisa and negative in immunofluorescenc ... | 1986 | 3537133 |
| effect of infection of the genital tract on the concentration of igg and albumin in bull serum and semen. | bovine igg and albumin concentrations were determined from serum and semen of 59 bulls that were divided into 4 groups: 35 non-infected bulls (group 1); 10 with vesiculitis due to corynebacterium pyogenes (group 2); 10 bulls with orchitis due to chlamydia psittaci (group 3); and 4 bulls with infectious vesiculitis (group 4) sampled both before and after antibiotic treatment. serum igg concentrations (25 mg/ml approximately) were similar in non-infected (gp 1) and infected bulls (gp 2,3,4) wherea ... | 1986 | 3541363 |
| comparison of five ovine isolates of chlamydia psittaci: an evaluation of three cell culture treatments. | | 1986 | 3543589 |
| serological detection of phage infection in chlamydia psittaci recovered from ducks. | antiserum prepared against a phage which infects a chlamydia psittaci isolate recovered from domestic ducks was used to screen other recent avian c psittaci isolates by indirect immunofluorescence. two more phage infected strains from ducks were discovered. however, phage was not detected in every isolate examined from common source ducks, although such birds are likely to be infected with the same c psittaci strain. moreover, phage could not always be demonstrated by indirect immunofluorescence ... | 1986 | 3544465 |
| comparison of some ovine chlamydia psittaci isolates by indirect immunofluorescence. | four ovine abortion isolates, including the a22 vaccine isolate, and an isolate from a case of lamb arthritis, were compared by indirect immunofluorescence using antisera raised in chickens and mice. cross titrations with homologous and heterologous antisera showed a one-way reaction between the single chlamydial isolate from a lamb with arthritis and the four isolates from cases of ovine enzootic abortion. the abortion isolates could not be distinguished. | 1987 | 3544468 |
| simultaneous vaccination by three living attenuated strains of brucella, salmonella and chlamydia in mice. | associations of vaccines for simultaneous administration were successfully developed long ago, in particular in human medicine, although there have been very few attempts to associate living bacterial vaccines. unfortunately, living vaccines can interact with one another resulting in either a virulent infection or an exclusion of one by the other. having developed two new low virulence vaccinal strains of chlamydia psittaci var. ovis and salmonella abortus ovis for use in veterinary medicine, we ... | 1987 | 3577354 |
| experimental infection of sheep with chlamydia psittaci. | | 1987 | 3594193 |
| atypical pneumonia: recognition and treatment. | while the term "atypical pneumonia" has been in use for many years, it cannot in fact be defined. however, there is a persuasive reason to retain the clinical use of the term, and that is to provide a guide for the clinician in the choice of empirical antibiotic therapy for patients with acute pneumonia. atypical pneumonia, then, is a descriptive term for a common clinical syndrome. provided certain clinicoepidemiological groups are excluded, the most common infectious causes of this syndrome ar ... | 1987 | 3600471 |
| [the cat as a possible infection source for chlamydia psittaci keratoconjunctivitis in humans]. | acute follicular keratoconjunctivitis in a 25-year-old female patient was diagnosed as an infection with chlamydia psittaci supposedly originating from a household cat with similar symptoms. the diagnosis was based on clinical symptoms, the appearance of chlamydia-psittaci-specific igm in the patient and the cat, and excluded other pathogenic agents. chlamydiae organisms could not be isolated, probably due to prior antibiotic treatment. two weeks of continuous treatment led to complete recovery ... | 1987 | 3617045 |
| prophylactic effects of silver nitrate and erythromycin on chlamydia psittaci conjunctivitis. | an animal model has been developed to study the effects of various prophylaxis agents on acquisition of chlamydial conjunctivitis. when hartley strain newborn guinea pigs received ocular inoculations of chlamydia psittaci followed by the instillation of various agents, 1% agno3 significantly lowered the risk of developing chlamydial conjunctivitis if it was administered within 15 min after the inoculation with c. psittaci. however, if the agno3 was administered at either 1 hr or 2 hr following i ... | 1987 | 3623840 |
| monoclonal antibody typing of chlamydia psittaci strains derived from avian and mammalian species. | a total of 77 chlamydia psittaci strains of avian, human, and mammalian origin were grouped into four serovars with 11 monoclonal antibodies recognizing the lipopolysaccharide and the major outer membrane protein antigens. the avian and human strains, which were closely related to each other, were distinct from the mammalian strains. immunological typing of c. psittaci with monoclonal antibodies seems practical. | 1987 | 3667918 |
| prevalence of chlamydia, toxoplasma, toxocara and ringworm in farm cats in south-west england. | the prevalence of infection with chlamydia psittaci, toxoplasma gondii, toxocara cati and microsporum canis was examined in 51 cats on 22 sheep farms in the bristol area. serum antibody to c psittaci and t gondii was present in 45 per cent and 47 per cent of cats, respectively. at the time of sampling c psittaci was isolated from 6 per cent of the cats, t cati was identified in 63 per cent of faecal samples but neither t gondii nor m canis was isolated. when examined according to the farm of ori ... | 1987 | 3672827 |
| survey for antibodies to chlamydia psittaci in cattle in hokkaido. | | 1987 | 3682527 |
| psittacosis in the rsa. | patients usually provisionally diagnosed as having typhoid fever or pneumonia are regularly admitted to the rietfontein fever hospital suffering from psittacosis. the main symptoms are intense headache, chills and fever and an irritating non-productive cough. later most patients develop signs of pneumonitis most clearly seen on radiographic examination. an important clue to the diagnosis is a history of contact with birds, most often budgerigars and more recently cockatiels. the diagnosis may be ... | 1986 | 3704861 |