| eosinophilic cholecystitis in association with clonorchis sinensis infestation in the common bile duct. | | 1999 | 10484225 |
| worm recovery and precipitin antibody response in guinea pigs and rats infected with clonorchis sinensis. | guinea pigs (hartley strain) and rats (wistar strain) were each fed 200 and 100 clonorchis sinensis metacercariae, respectively. five animals from each species were sacrificed weekly between 1-8 weeks postinfection (wpi) and then at 12, 16, 20 and 30 wpi for collection of worms, bile and sera. the overall worm recovery rates for guinea pigs and rats were 18.7% and 12.4%, respectively. only one of the five rats examined at 20 wpi still harbored one worm, while all were worm-free at 30 wpi. by a d ... | 1998 | 10496161 |
| helminthic food-borne infections. | the importance of food-borne helminthic infections is not well recognized and has not received the same attention as food-borne bacterial infections. poverty, overpopulation, and cultural practices contribute to conditions that maintain food-borne helminthic infections. the development of better means of transportation and the ease of reaching otherwise inaccessible markets have increased significantly our risk of coming in contact with food containing infectious organisms. the education of indu ... | 1999 | 10549430 |
| little effect of praziquantel or artemisinin on clonorchiasis in northern vietnam. a pilot study. | the first choice for treatment of clonorchis sinensis infections is praziquantel. experimental data suggest that artemisinin derivatives are active against c. sinensis. the efficacy of both drugs against clonorchiasis was evaluated in a pilot study in clonorchiasis patients in an endemic area in the north of vietnam. twenty-one patients received praziquantel 25 mg/kg o.d. for three days, the regular regimen in that area, and 21 patients were treated with artemisinin 500 mg b.i.d. for 5 days. fae ... | 1999 | 10632989 |
| usefulness of igg4 subclass antibodies for diagnosis of human clonorchiasis. | the present study analyzed serum igg subclass antibody reaction to major antigenic bands of clonorchis sinensis to investigate improvement of its serodiagnosis. of the four subclass antibodies, igg1 and igg2 antibodies were produced but not specific, igg3 antibody was least produced, and igg4 antibody was prominent and specific. the serum igg antibody reaction to any of 43-50, 34-37, 26-28, and 8 kda bands was found in 65.5% of 168 egg positive cases while igg4 antibody reaction was found in 22. ... | 1999 | 10634040 |
| infection status with trematode metacercariae in pond smelts, hypomesus olidus. | many koreans usually eat raw pond smelts, hypomesus olidus, in the winter. this study was performed to evaluate the infection status with trematode metacercariae in pond smelts from january 1998 through february 1999. among 1,305 fish collected, 459 were purchased from wholesale dealers in chinchon-gun, chungchongbuk-do, and the rest of them were caught with a casting net in soyangho (lake), taehoman (bay) and paekkokchosuchi (pond). seven species of trematode metacercariae including two unident ... | 2000 | 10743358 |
| isozyme electrophoresis patterns of the liver fluke, clonorchis sinensis from kimhae, korea and from shenyang, china. | an enzyme analysis of the liver fluke, clonorchis sinensis from kimhae, korea and from shenyang, china was conducted using a horizontal starch gel electrophoresis in order to elucidate their genetic relationships. a total of eight enzymes was employed from two different kinds of buffer systems. two loci from each enzyme of aconitase and esterase (alpha-na and beta-na); and only one locus each from six enzymes, glucose-6-phosphate dehydrogenase (g6pd), alpha-glycerophosphate dehydrogenase (gpd), ... | 2000 | 10743360 |
| isolation of liver oval cells from hamsters treated with diethylnitrosamine and 2-acetyl aminofluorene. | recently, cholangiocellular carcinoma (ccc) was successfully induced in the hamster by infecting with clonorchis sinensis following hepatocarcinogen treatment and has been proposed as a suitable model to study the pathogenesis of human ccc. in this hamster model, oval cells are suggested to be cells of origin of ccc. more direct analysis of histogenesis of ccc would become possible if large numbers of highly purified oval cells of hamster origin are obtained. in this study, we describe successfu ... | 2000 | 10770596 |
| molecular cloning and immunological characterization of phosphoglycerate kinase from clonorchis sinensis. | the parasite clonorchis sinensis was determined to utilize a large amount of external glucose to carry its energy metabolism. phosphoglycerate kinase (pgk), a glycolytic enzyme, found in many parasites, has been identified as one of the candidate molecules distinguished from human counterparts for vaccine and drug developments. a cdna clone purified by screening a c. sinensis cdna library using a heterologous cdna probe encoded a putative peptide of 415 amino acids with over 60% identities with ... | 2000 | 10838223 |
| partial characterization of a 17 kda protein of clonorchis sinensis. | a 17 kda protein from clonorchis sinensis adults was purified by a procedure including sephacryl s-200 hr gel filtration and q-sepharose anion exchange chromatography. the protein was proved to be a cysteine protease as it showed hydrolytic activity toward cbz-phe-arg-amc in the presence of dithiothreitol and was inhibited by specific inhibitors such as iodoacetic acid or trans epoxy-succinly-l-leucyl-amido(4-guanidino) butane. the polyclonal antibody raised against the protein reacted to 17 kda ... | 2000 | 10905071 |
| chromosomes of the liver fluke, clonorchis sinensis. | a karyological study was carried out in order to compared the chromosome numbers, chromosome morphologies and karyotypes of the oriental liver fluke, clonorchis sinensis (trematoda: opisthorchiidae), collected from korea and china. chromosome preparations were made by means of air-drying method. the chromosome number was 2n = 56 in both korean and chinese flukes, and chromosomes were divided into two groups based on this size; consisting of 8 pairs of large and 20 pairs of small chromosomes. how ... | 2000 | 11002660 |
| separation of calcareous corpuscles from plerocercoids of spirometra mansoni and their binding proteins. | calcareous corpuscles, known as mineral concretions in platyhelminthes, may have special biological functions. in the present study we succeeded in the quantitative separation of calcareous corpuscles in plerocercoids of spirometra mansoni (sparganum) using ficoll. purified calcareous corpuscles were reacted with crude extracts of platyhelminthes, including sparganum. proteins commonly binding to calcareous corpuscles were molecules of 10, 17, 22, and 35 kda. a 95-kda molecule was found to bind ... | 2000 | 11002990 |
| composite neuroendocrine and adenocarcinoma of the common bile duct associated with clonorchis sinensis: a case report. | the biliary tract has neuroendocrine cells of endoderm origin similar to the gastrointestinal tract, however neuroendocrine tumors of the biliary tract are rare. we report a composite glandular-endocrine cell carcinoma of the common bile duct in a 64-year-old korean man which was associated with clonorchis sinensis. the patient complained of right upper quadrant abdominal pain. several parasites of clonorchis sinensis were removed during the percutaneous transbiliary drainage. endoscopic retrogr ... | 2000 | 11020854 |
| identification of a clonorchis sinensis gene encoding a vitellaria antigenic protein containing repetitive sequences. | | 2000 | 11087931 |
| update on hepatobiliary and pulmonary flukes. | hepatobiliary and pulmonary trematodes continue to cause significant morbidity and mortality worldwide. this article provides a selected update on the clinical features, diagnosis, immunology, treatment, and control of infections caused by clonorchis sinensis, opisthorchis species, fasciola hepatica, and paragonimus species. although recent molecular studies have elucidated novel mechanisms of immune evasion in paragonimus westermani and f. hepatica, there are no immediate prospects for a vaccin ... | 1999 | 11095819 |
| use of liver graft infested with clonorchis sinensis for living related liver transplantation: a case report. | | 2000 | 11120123 |
| shared epitope for monoclonal ir162 between anisakis simplex larvae and clonorchis sinensis and cross-reactivity between antigens. | ir162 is a rat monoclonal ige antibody (mab). in the investigation of rat ige production, the antigens recognized by ir162 (ir-ags) were found to be expressed by a variety of helminthic parasites. by western blot analysis, ir162 detected bands in crude extracts of anisakis simplex larvae, clonorchis sinensis, paragonimus westermani, plerocercoids of spirometra mansoni, and toxocara canis. excretory-secretory material from a. simplex larvae also contained a protein recognized by ir162. ir162 mab ... | 2000 | 11128497 |
| differentiation of hamster liver oval cell following clonorchis sinensis infection. | oval cells which appear in the liver after hepatic injuries are suspected to be progenitor cells for both hepatocytes and bile duct cells. oval cell isolated from the livers of the hamsters treated with diethylnitrosamine and 2-acetylaminofluorene and infected with clonorchis sinensis (cs). cultured for 2 weeks and evaluated for differentiation and plasticity by electron microscopy and immunohistochemistry. in the cs-uninfected group, glycogen granules and peroxisomes were noted in the cells tha ... | 2000 | 11193347 |
| crystallization of clonorchis sinensis 26 kda glutathione s-transferase and its fusion proteins with peptides of different lengths. | a clonorchis sinensis 26 kda glutathione s-transferase (csgst) and its fusion proteins containing 14 and 48 amino-acid peptides at the n-terminus have been crystallized using polyethylene glycol monomethylether 550 as a precipitant. crystals of the three proteins show very similar crystal properties: they diffract to at least 2.3 a resolution and belong to the orthorhombic space group p2(1)2(1)2(1). the unit-cell parameters of csgst crystals were a = 66.64 (1), b = 68.91 (1), c = 123.41 (2) a, w ... | 2001 | 11264588 |
| molecular cloning and characterization of an antigenic protein with a repeating region from clonorchis sinensis. | in the course of immunoscreening of clonorchis sinensis cdna library, a cdna csrp12 containing a tandem repeat was isolated. the cdna csrp12 encodes two putative peptides of open reading frames (orfs) 1 and 2 (csrp12-1 and -2). the repetitive region is composed of 15 repeats of 10 amino acids. of the two putative peptides, csrp12-1 was proline-rich and found to have homologues in several organisms. recombinant proteins of the putative peptides were bacterially produced and purified by an affinit ... | 2001 | 11301591 |
| infectivity and pathological changes in murine clonorchiasis: comparison in immunocompetent and immunodeficient mice. | the main complications of clonorchiasis are periportal inflammation, biliary hyperplasia, periductal fibrosis, and subsequently the development of biliary tumors in the liver. this study was undertaken to compare the infectivity and histopathologic changes between in immunocompetent fvb/nj and balb/ca strains, and immunodeficient severe combined immunodeficient (scid) and athymic nude mice after the metacercariae of clonorchis (c.) sinensis were infected. the experiment showed that c. sinensis w ... | 2001 | 11346177 |
| molecular cloning and characterization of a mu-class glutathione s-transferase from clonorchis sinensis. | in biliary passages, clonorchis sinensis causes epithelial hyperplasia and is assumed to promote carcinogenesis. glutathione s-transferase (gst) is an antioxidant enzyme involved in phase ii defense in trematodes. a clone (pcsgstm1) encoding a gst was identified by screening a c. sinensis cdna library with a pcr-synthesized cdna probe. the predicted amino acid sequence encoded by pcsgstm1 cdna had a high degree of sequence identity and folding topology similar to the mu-class gsts. the estimated ... | 2001 | 11377741 |
| clonorchis sinensis: molecular cloning and characterization of 28-kda glutathione s-transferase. | a 28-kda glutathione s-transferase (cs28gst) was purified from a clonorchis sinensis cytosolic fraction through anion-exchange and glutathione-affinity column chromatographies. a monoclonal antibody raised against cs28gst reacted specifically to the c. sinensis antigen among trematode proteins. a putative peptide of 212 amino residues deduced from a cdna clone appeared homologous with 28-kda gst of trematodes, and its secondary structural elements predicted a gsh-binding site. recombinant cs28gs ... | 2001 | 11384162 |
| csrn1, a novel active retrotransposon in a parasitic trematode, clonorchis sinensis, discloses a new phylogenetic clade of ty3/gypsy-like ltr retrotransposons. | we screened the genome of a trematode, clonorchis sinensis, in order to identify novel retrotransposons and thereby provide additional information on retrotransposons for comprehensive phylogenetic study. considering the vast potential of retrotransposons to generate genetically variable regions among individual genomes, randomly amplified polymorphic dnas (rapds) detected by arbitrarily primed polymerase chain reactions were selected as candidates for retrotransposon-related sequences. from rap ... | 2001 | 11470838 |
| epidemiological significance of sero-positive inhabitants against sparganum in kangwon-do, korea. | sparganum is a plerocercoid of pseudophyllidean tapeworms, diphyllobothrium or spirometra spp. human sparganosis is endemic mainly in east and southeast asian countries where the custom of eating raw snake or frog meat, or poulticing with snake's skin exists. from january 1995 to november 1999, an epidemiological survey was undertaken to evaluate the serum levels of anti-sparganum specific igg antibodies in whachon-gun residents, korea. an enzyme- linked immunosorbent assay (elisa) and immunoblo ... | 2001 | 11519077 |
| trematodes of the family opisthorchiidae: a minireview. | examination of the original descriptions of genera placed within the family opisthorchiidae has revealed that only 33 of the original 43 genera are valid members of this family. further study of these descriptions should also reveal that many of the subfamilies are also invalid. with reference to the original descriptions of these genera, and subsequent literature, a brief survey of the family has been compiled. information on the spectrum of definitive hosts that these helminths parasitize is p ... | 2001 | 11590910 |
| current status of human parasitic infections in taiwan. | the eradication of the 2 mosquito-borne parasitic diseases, malaria and lymphatic filariasis, is one of the greatest achievements of the parasite control campaigns in taiwan. most of the soil-transmitted nematode infections, with the exception of pinworm infection, are currently well controlled and limited to some aboriginal areas. food-borne parasitic zoonosis such as infections with angiostrongylus cantonensis, clonorchis sinensis, and taenia saginata asiatica are not rare, but the former is s ... | 2001 | 11605804 |
| cystatin capture enzyme-linked immunosorbent assay for serodiagnosis of human clonorchiasis and profile of captured antigenic protein of clonorchis sinensis. | enzyme-linked immunosorbent assay (elisa) with crude extracts of adult clonorchis sinensis has been reported to have a high degree of sensitivity with a moderate degree of specificity for the serodiagnosis of clonorchiasis. the cystatin capture elisa was investigated for its usefulness for the serodiagnosis of human clonorchiasis. cystatin bound specifically to cysteine proteinases in crude extracts of adult c. sinensis worms, and its binding capacity was not hindered competitively by the other ... | 2001 | 11687443 |
| antigenic profile and localization of clonorchis sinensis proteins in the course of infection. | in the course of clonorchis sinensis infection, antigens presented to the hosts may be in a close relation to growth of the fluke. the antigenic proteins stimulating igg antibody production were chronologically identified by immunoblot and localized by immunohistochemical staining. in the early stage of infection until 12 weeks post-infection (pi), antigens were proteins with molecular mass larger than 34 kda which were derived from the tegument, testes and intrauterine eggs. after 20 weeks pi, ... | 2001 | 11775331 |
| infection rates of enterobius vermicularis and clonorchis sinensis of primary school children in hamyang-gun, gyeongsangnam-do (province), korea. | the egg positive rate of enterobius vermicularis and clonorchis sinensis of school children in the rural area was studied in hamyang-gun, gyeongsangnam-do in korea. cellotape anal swab and formalin ether concentration methods were performed one time to 720 primary school children. the total egg positive rate of e. vermicularis was 12.6% in two schools (baekjeon and wiseong). in the baekjeon and wiseong primary school, the egg positive rate of e. vermicularis was 4.6% and 13.4%, respectively. pin ... | 2001 | 11775334 |
| characterization and large-scale expression of the recombinant cysteine proteinase from adult clonorchis sinensis. | cysteine proteinases play important roles in the pathogenesis of several parasitic infections and have been proposed as targets for the structure-based approach of drug design. as the first step toward applying this strategy to design inhibitors as antiparasitic agents for clonorchis sinensis, we overexpressed and characterized the 24-kda cysteine proteinase from adult worms. first, the partial cysteine proteinase gene from c. sinensis was cloned by performing reverse transcription polymerase ch ... | 2001 | 11780837 |
| parasitic infections and hepatic neoplasia. | parasitic infections are highly prevalent in the general population. detecting a relationship between a parasitic infection and cancer is not an easy task; it requires epidemiological, microbiological and molecular biology techniques. in this article the important mechanisms by which parasitic infections may induce carcinogenesis are reviewed. the association between schistosoma japonicum, schistosoma mansoni and hepatocellular carcinoma is covered. the cause-and-effect relationship between the ... | 2001 | 11935088 |
| epidemiological study of clonorchiasis and metagonimiasis along the geum-gang (river) in okcheon-gun (county), korea. | the endemic status of clonorchiasis and metagonimiasis along the geum-gang (river) in okcheon-gun (county) in korea was examined. from february to december 2000, stools of total 1,081 inhabitants living in 5 villages were examined. each stool specimen was examined by both the cellophane thick smear method and the formalin-ether sedimentation technique. egg-positive cases were further analyzed by stoll's egg-counting technique, and praziquantel was administered to positive cases. the egg-positive ... | 2002 | 11949215 |
| motility of minute intestinal fluke, haplorchinae spp, metacercariae in fish dishes prepared by different uncooked methods. | fish-borne trematode is a worldwide problem, with the number of people infected by liver flukes alone estimated at 21 million. in addition to the major liver flukes, opisthorchis viverrini and clonorchis sinensis, several types of intestinal flukes also use cyprinoid fish as the intermediate host. traditional ingestion of undercooked and uncooked fish preparations is a major factor in pathogen acquisition. | 2002 | 11965210 |
| haplorchis infections in intermediate hosts from a clonorchiasis endemic area in meinung, taiwan, republic of china. | snails and freshwater fish were examined from four ponds in the meinung township in which clonorchis sinensis was known to be endemic 18 years ago. no metacercariae were found in 478 tilapia nilotica, whereas of 451 ctenopharyngodon idellus examined, 16.2%, 3.3% and 0.9% were found to be infected with haplorchis pumilio, h. taichui and clonorchis sinensis, respectively. in addition, there were some unidentified metacercariae in 12.0% of ctenopharyngodon idellus examined. overall, no positive cor ... | 2002 | 12015833 |
| geographical variation of the liver fluke, clonorchis sinensis, from korea and china based on the karyotypes, zymodeme and dna sequences. | genetic characterization was carried out in order to reveal the geographical variations of the oriental liver fluke, clonorchis sinensis (trematoda: opisthorchiidae), collected in korea and china. the chromosome number was 2n=56 in both korean (kimhae) and chinese (liaoning) flukes, and chromosomes were divided into two groups based on their sizes; consisting of 8 pairs of large and 20 pairs of small chromosomes. however, the karyotypes showed some differences between korean and chinese flukes. ... | 2001 | 12041573 |
| nucleotide sequence of mitochondrial co i and ribosomal its ii genes of opisthorchis viverrini in northeast thailand. | the mitochondrial cytochrome c oxidase subunit i (co i) gene and the second internal transcribed spacer region (its ii) gene of opisthorchis viverrini were compared among o. viverrini from various areas in northeast thailand. the nucleotide sequences of partial co i gene (417 bp) of o. viverrini differed among o. viverrini originated from ubon ratana, leongpleuy, ban phai, maha sarakham, and chatturat. these intraspecific variations were classified into 5 patterns but no area-specific pattem was ... | 2001 | 12041584 |
| epidemiology and control of clonorchiasis sinensis in china. | | 2001 | 12041609 |
| [multiple cancer of the common bile duct associated with clonorchiasis]. | | 2002 | 12048897 |
| characterization of partially purified 8 kda antigenic protein of clonorchis sinensis. | the 8 kda antigenic protein of clonorchis sinensis was partially purified by ammonium sulfate precipitation and subsequently by a column chromatographic steps. the purified protein was separated into 7 and 8 kda protein bands through sds-tricine gel electrophoresis, while the protein was found to migrate to a 8 kda band in 7.5-15% sds-page. the molecular weight of the antigen was estimated to be 110 kda by superose 6 hr 10/30 gel filtration. the purified antigen strongly reacted with the human s ... | 2002 | 12073733 |
| [preparation and characterization of monoclonal antibodies against clonorchis sinensis]. | to develop and identify monoclonal antibody (mcab) against adult clonorchis sinensis. | 1998 | 12078207 |
| [advances in the study of diagnostic antigens in clonorchiasis]. | | 1998 | 12078264 |
| sclerosing cholangitis caused by clonorchis sinensis. | | 2002 | 12085048 |
| purification and characterization of a 7-kda protein from clonorchis sinensis adult worms. | a 7-kda protein was purified from extracts of adult clonorchis sinensis by a combination of ammonium sulfate precipitation, anion exchange chromatography, cation exchange chromatography, gel-filtration chromatography, and reversed-phase fplc. the 7-kda protein exists in the excretory-secretory products of adult c. sinensis, but not in extracts of adult paragonimus westermani. also, the 7-kda protein reacted with the sera of patients with clonorchiasis but not with paragonimiasis or normal human ... | 2002 | 12099418 |
| liver fluke-associated cholangiocarcinoma. | infection with liver flukes has been reported to be associated with bile duct malignancy. | 2002 | 12153620 |
| hemolytic activity and developmental expression of pore-forming peptide, clonorin. | peptides pore-forming in cell membrane have been identified from a wide range of animals. a putative pore-forming peptide deduced from a cdna clone of clonorchis sinensis (clonorin) was predicted to consist of four amphipathic alpha-helices. clonorin contained six invariably conserved cysteine residues, identified to form three disulfide bonds. these predicted structural features are highly homologous with pore-forming peptides, the amoebapores. recombinant clonorin showed hemolytic activity tow ... | 2002 | 12207906 |
| expression of cysteine proteinase of clonorchis sinensis and its use in serodiagnosis of clonorchiasis. | a gene encoding cysteine proteinase from clonorchis sinensis has been cloned and expressed in escherichia coli. the cysteine proteinase cdna fragment was amplified by reverse transcription-polymerase chain reaction using degenerate oligonucleotide primers derived from conserved active site of cysteine proteinases. the 5' and 3' regions of the gene were amplified using rapid amplification of cdna ends. the cloned gene has an open reading frame of 696 bp and deduced amino acid sequence of 232. seq ... | 2002 | 12435144 |
| extrahepatic cholangiocarcinoma associated with clonorchiasis: ct evaluation. | i evaluated the computed tomographic (ct) features of nine pathologically proven cases of cholangiocarcinoma in the extrahepatic bile duct associated with clonorchiasis. | 2003 | 12483388 |
| ultrastructural localization of 28 kda glutathione s-transferase in adult clonorchis sinensis. | glutathione s-transferase (28gst) with molecular mass of 28 kda is an antioxidant enzyme abundant in clonorchis sinensis. in adult c. sinensis, 28gst was localized in tegumental syncytium, cytons, parenchyma, and sperm tails examined by immunoelectron microscopy. c. sinensis 28gst was earlier found to neutralize bioreactive compounds and to be rich in eggs. accordingly, it is suggested that 28gst plays important roles in phase ii defense system and physiological roles in worm fecundity of c. sin ... | 2002 | 12509100 |
| infection status of clonorchis sinensis in residents of hamyang-gun, gyeongsangnam-do, korea. | oriental liver fluke (clonorchis sinensis) infection was surveyed among residents of hamyang-gun, gyeongsangnam-do, korea during the period of january 2001 to march 2002. total 1,041 stool samples were collected from residents who visited public health center and its branches in hamyang-gun and examined using formalin-ether sedimentation method. the overall egg positive rate was 16%, male showing higher positive rate (21%) than female (10%). the age group of 30 to 50 years had the highest egg po ... | 2002 | 12509104 |
| [clonorchiasis in the upper amur region: biology, epidemiology, clinical presentation]. | results of studies of clonorchiasis in the region of the upper amur and its tributaries are presented. biological features of the pathogen and its epidemiology are outlined. clinical symptoms were studied in 112 patients. in case of death, a pathoanatomic picture is given. | 2002 | 12557581 |
| [survey and treatment of clonorchiasis in fushan city]. | | 1999 | 12563871 |
| chemotherapeutic efficacy of praziquantel in rats with protective immunity to clonorchis sinensis infection. | to study the synergic effect of praziquantel (pzq) and host acquired immunity on clonorchis sinensis. | 2000 | 12567726 |
| [diagnosis of clonorchis sinensis infection by ultrasonography]. | | 2000 | 12567736 |
| proliferation and cytokine production of lymphocytes from clonorchis sinensis-infected rats in response to stimulators in vitro. | to study the rat lymphocyte proliferation, differentiation and cytokine production in response to clonorchis sinensis infection. | 2002 | 12567984 |
| [application of dot immunogold filtration assay for detecting serum antibodies in clonorchiasis patients]. | to establish a new, fast, sensitive and specific assay for detecting antibodies in clonorchiasis. | 2001 | 12571996 |
| [a case of clonorchis sinensis infection complicated with gallstone]. | | 2001 | 12572069 |
| living donor liver transplantation using graft infested with clonorchis sinensis: two cases. | | 2003 | 12591310 |
| clonorchis sinensis: glutathione s-transferase as a serodiagnostic antigen for detecting igg and ige antibodies. | human clonorchis sinensis infection is endemic in east asian countries. glutathione s-transferases (gsts) are anti-oxidant enzymes found in all living creatures as well as in trematodes. in this study, we examined the recombinant 26kda gst protein of c. sinensis (cs26gst) for its serodiagnostic antigenicity toward igg and ige antibodies by elisa and immuno-enhanced chemiluminescence, respectively. in igg elisa, recombinant cs26gst showed 33.3% sensitivity and 100% specificity for trematode-infec ... | 2002 | 12594964 |
| excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by elisa. | although stool examination is the standard diagnostic method of clonorchiasis, serodiagnosis by elisa using crude antigen is now widely used because of its convenience. however, elisa diagnosis still suffers from cross-reactions, and therefore there is a need to improve the present conventional elisa. the present study was undertaken to evaluate the diagnostic value of elisa using excretory-secretory antigen (esa) instead of crude antigen (ca) of clonorchis sinensis. the diagnostic sensitivity o ... | 2003 | 12666728 |
| effects of gamma-irradiation on the infectivity and chromosome aberration of clonorchis sinensis. | effects of gamma irradiation on the worm survival and chromosomal aberration of clonorchis sinensis were studied. the metacercariae irradiated with various amounts of gamma radiation (ranging from 5 gy to 50 gy) were fed to rats, and the effects were compared with those of non-irradiated controls. recovery rates of adult worms in irradiated groups were reduced gradually as increasing of the irradiation doses. no worm was recovered from rats which were fed with 50 gy irradiated metacercariae. the ... | 2003 | 12666729 |
| detection of parasite eggs from archaeological excavations in the republic of korea. | excavations at two sites dating from 2000 bc-1900 ad in southeastern areas of the republic of korea, revealed the remains of several structures. examination of the contents suspected privies revealed the presence of eggs from 5 kinds of parasite: ascaris, trichuris, clonorchis, and two species of unknown trematodes. clonorchis sinensis eggs were found in a soil dating from around ad 668-935. this is the first record of c. sinensis eggs in archaeological materials in the republic of korea. | 2003 | 12687771 |
| molecular cloning and immunolocalization of the 17 kda myoglobin of clonorchis sinensis. | we purified the 17 kda protein abundant in clonorchis sinensis crude extracts. the n-terminal amino acid sequence of this protein was determined and an oligonucleotide probe synthesized. using this probe, the cdna encoding the protein was cloned and sequenced from the c. sinensis cdna library. it was found to consist of a total of 150 amino acids and to have 41% conserved homology with the myoglobin of the trematodes paramphistomum epiclitum and isoparorchis hypselobagri. the gene product over-e ... | 2003 | 12720088 |
| ultrastructural localization of phosphoglycerate kinase in adult clonorchis sinensis. | phosphoglycerate kinase (pgk) is an enzyme that produces one atp molecule in the glycolytic pathway. clonorchis sinensis is largely dependent on glycolysis for energy production. we performed immunoelectron microscopy on adult c. sinensis by using mouse immune serum raised against recombinant c. sinensis pgk. a high density of gold particles was found in the microvilli of the intestinal epithelium and in lamellae of the sperm duct. pgk was common in the somatic cells of intra-uterine eggs and in ... | 2003 | 12720089 |
| boudicca, a retrovirus-like long terminal repeat retrotransposon from the genome of the human blood fluke schistosoma mansoni. | the genome of schistosoma mansoni contains a proviral form of a retrovirus-like long terminal repeat (ltr) retrotransposon, designated boudicca: sequence and structural characterization of the new mobile genetic element, which was found in bacterial artificial chromosomes prepared from s. mansoni genomic dna, revealed the presence of three putative open reading frames (orfs) bounded by direct ltrs of 328 bp in length. orf1 encoded a retrovirus-like major homology region and a cys/his box motif, ... | 2003 | 12743272 |
| lethal effect of ammonia on metacercariae of clonorchis sinensis. | the effect of endogenous ammonia produced in dead fish or ammonia in ammonium bicarbonate solutions was evaluated on the viability of clonorchis sinensis metacercariae. viability was determined by worm recovery in rats infected with metacercariae exposed to ammonia in decaying fish or in vitro solutions. the recovery rates were 58%, 48%, 44% and 2% in fish that had been allowed to decay for 5, 10, 20 and 30 days at 4 degrees c, respectively, and this rate ( y) was inversely correlated with the a ... | 2003 | 12750995 |
| use of a recombinant clonorchis sinensis pore-forming peptide, clonorin, for serological diagnosis of clonorchiasis. | a recombinant pore-forming peptide of clonorchis sinensis, clonorin, was evaluated for serodiagnostic reagent of clonorchiasis by enzyme-linked immunosorbent assay detecting igg antibody. recombinant clonorin showed 100% specificity and low sensitivity for sera of human clonorchiasis. in contrast, c. sinensis crude antigen revealed lower specificity and higher sensitivity than recombinant clonorin did. in sera of experimental rabbits, clonorin-specific igg antibody was increased remarkably 8 wee ... | 2003 | 12798930 |
| [mixed infection of strongyloides stercoralis and clonorchis sinensis: report of one case]. | objective: we report a case of mixed infection of strongyloides stercoralis and clonorchis sinensis, the diagnosis of which was difficult due to lack of specific clinical and imaging features. definitive diagnosis was finally reached on the basis of detection of the larvae and eggs in the stool of the patient. timely and effective therapy is of much importance for the prognosis of the disease. | 2003 | 12810401 |
| changes of anti-clonorchis sinensis igg antibody in serum after praziquantel treatment in human clonorchiasis. | anti-clonorchis igg antibody levels in serum were observed by elisa in 129 egg positive cases and in 25 controls. the antibody levels were 0.063 to 1.216 (0.325+/-0.202)in clonorchiasis cases and 0.078 to 0.670 (0.255+/-0.133) in controls. the difference was statistically significant. however, serological diagnosis of clonorchiasis was not satisfactory in lightly infected cases because of low levels of specific igg antibody. the antibody levels were well correlated with epg. changes of the igg a ... | 1988 | 12811027 |
| intensity of infection and development of adult clonorchis sinensis in hamsters. | in an attempt to determine the susceptibility of inbred golden hamsters(mesocricetus auratus) to the experimental infection with clonorchis sinensis, twenty-five hamsters were divided into 5 groups and administered orally 5, 10, 20, 30 or 50 metacercariae each. the hamsters were killed on the 45th day after infection. the adult flukes were recovered from all hamsters. the overall recovery rate for the fluke was 57.9 percent with the range of 48.4 percent to 92.0 percent. as the number of the met ... | 1988 | 12811028 |
| a human case of centrocestus armatus infection in korea. | a human case of centrocestus armatus (heterophyidae) infection was proved by identifying an adult worm collected after treatment with praziquantel in korea. the case is 42-year old man who resides in a rural area in sanchung-gun, kyeongsangnam-do. the case was concomitantly infected with clonorchis sinensis and had the history of eating raw freshwater fishes including zacco platypus, which are known to be the second intermediate host of c. armatus in korea. this is the first report of natural hu ... | 1988 | 12811033 |
| changing patterns of infection with digenetic larval trematodes from fresh-water fish in river taewha, kyongnam province. | recent patterns of infection with digenetic larval trematodes in fresh-water and brackish-water fish were studied in three locations of the river taewha during the period from april to october 1988, and compared with the data reported previously in the same river. of 16 species of fish examined, the encysted larvae of cyathocotyle orientalis were found most frequently from 9 species of fresh-water fish. the metacercariae of echinochasmus sp., metacercaria hasegawai and metagonimus yokogawai were ... | 1988 | 12811040 |
| the wormicidal substances of fresh water fishes on clonorchis sinensis vii. the effect of linoleic acid and ethyl linoleate on parasite viability. | in an attempt to analyze the clonorchicidal activity of linoleic acid and ethyl linoleate in vitro, the wormicidal effects on clonorchis sinensis were chronologically monitored in dose titration experiments. excysted metacercariae were killed within a period of 31.0+/-4.0 min, 149.3+/-4.l min and 207.0+/-13.5 min with 100.0 mg, 0.1 mg and 0.001 mg linoleic acid, respectively. the time required for the linoleic acid to kill adult worms was 167.0+/-0.8 min with 100.0 mg, 253.0+/-0.8 min with 0.1 m ... | 1988 | 12811045 |
| [studies on the immunodiagnosis of rabbit clonorchiasis ii. immunoaffinity purification of whole worm antigen and characterization of egg, metacercaria and adult antigens of clonorchis sinensis] | the sensitivity and specificity of crude and affinity-purified antigens of clonorchis sinensis obtained from the infected rabbits were studied. stage-specific antigenic proteins from the eggs, metacercariae and adult worms were characterized by sds-polyacrylamide gel electrophoresis (sds-page) and enzyme-linked immunosorbent assay (elisa) the results were as follows: the antibody-binding antigen (aba) purified from whole worm crude antigen (wwa) by cnbr-activated sepharose 4b affinity chromatogr ... | 1988 | 12811052 |
| [cercarial chaetotaxy of clonorchis sinensis] | the pattern of sensory papillae, chaetotaxy, of the cercaria of clonorchis sinensis was observed. the chaetotaxy was as follows; 5-6 ci l, 4-5 ci 2, 5-6 ci 3 at lst row, 4 cii 1, 2 cii 2, 4 cii 3, 5-6 cii 4 at 2nd row, 3-4 ciii 1, 2-3 ciii 2 at 3rd row, and 2 civ 1, 2-3 civ 2, 2-3 civ 3, at 4th row, in cephalic region; 2 aiv, l aid, 2 aiiv, 1 aiid, 2 aiiiv, 2 aiiid, 1 aivv, 1 aivd, 1 piid, l piiid, in ventral(v) and dorsal(d) portions of body. caudal region revealed 2-2-2-2 formula. | 1988 | 12811056 |
| cloning of a pore-forming subunit of atp-sensitive potassium channel from clonorchis sinensis. | a complete cdna sequence encoding a pore-forming subunit (kir6.2) of atp-sensitive potassium channel in the adult worm, clonorchis sinensis, termed cskir6.2, was isolated from an adult cdna library. the cdna contained a single open-reading frame of 333 amino acids, which has a structural motif (a gfg-motif) of the putative pore-forming loop of the kir6.2. peculiarly, the cskir6.2 shows a lack-sequence structure, which deleted 57 amino acids were deleted from its n-terminus. the predicted amino a ... | 2003 | 12815327 |
| experimental clonorchiasis in dogs: ct findings before and after treatment. | to determine whether disease activity in clonorchiasis can be assessed with dynamic computed tomography (ct). | 2003 | 12832577 |
| clonorchis sinensis: immunolocalization of 26 kda glutathione s-transferase in adult worms. | a mu-class glutathione s-transferase (cs26gst) of molecular mass 26 kda was characterized from clonorchis sinensis. in adult c. sinensis, the distribution of the cs26gst was investigated by immuno-histochemistry and electron microscopy. cs26gst was localized to the tegument and parenchyma. immunogold labeling was strong in the tegumental cell bodies and moderate in the tegument and ova in the oviduct. it is suggested that cs26gst plays a role in the metabolism and fecundity of c. sinensis. | 2002 | 12856316 |
| [research progress in molecular biology of important proteins of clonorchis sinensis]. | | 2003 | 12884596 |
| [effect of aflatoxin b1 on ultrastructural changes of biliary epithelial cells in mice experimentally infected with clonorchis sinensis] | the present study was carried out to examine the effect of a carcinogen, aflatoxin b1 on the ultrastructural changes of biliary epithelial cells in mice infected with clonorchis sinensis. a total of 93 male albino mice(balb/c strain) was divided into 3 groups; group i, treated with 1.0 ppm aflatoxin b1 for 12 weeks; group ii, given 50 c. sinensis metacercariae, and group iii, given 50 metacercariae and treated with 1.0 ppm aflatoxin b1 for 12 weeks. three mice served for untreated-uninfected con ... | 1987 | 12886060 |
| histopathological changes of the liver after praziquantel treatment in clonorchis sinensis infected rabbits. | the rabbits were infected with clnorchis sinensis and were treated with praziquantel at the dose of 50 mg/kg x 2 x 2 days afer 1, 2, 4, 8 weeks and 7 months from the infection. their livers were observed histopathologically 1, 4 and 12 weeks after treatment. the findings are summarized as below: the changes of the liver in control rabbits were relatively mild until 2 weeks after infection. however, widening and thickening of bile ducts, proliferation of biliary epithelium and periductal fibrosis ... | 1987 | 12886061 |
| [larvicidal action of liquid nitrogen against metacercariae of clonorchis sinensis] | the author investigated the larvicidal action of liquid nitrogen against the metacercariae of clonorchis sinensis, with an observation on the freshness and change of taste in the flesh of fishes. the results obtained were as follows: the metacercariae in the flesh of pseudorasbora parva, which were treated with liquid nitrogen (-192c), were not killed within 10 seconds, but completely killed over 30 seconds. in comparison, the metacercariae in the flesh of p. parva kept in a refrigerator (-12c) ... | 1987 | 12886062 |
| [passive transfer of immunity against clonorchis sinensis by peritoneal exudate cells in mice] | this study was undertaken to evaluate the role of peritoneal exudate cells in the transfer of immunity against the liver fluke, clonorchis sinensis in the inbred balb/c mice. ten donor mice were divided into 2 groups. one group consisted of 5 mice was infected orally with 20 metacercariae of c. sinensis, and the other group was injected intraperitoneally with 20 excysted larvae. thirty days after immunization, the peritoneal exudate cells were obtained from the donor mice. twenty recipient mice ... | 1987 | 12886081 |
| [studies on beta-glucuronidase activities in liver, stomach and small intestinal tissues of rabbits infected with clonorchis sinensis] | the author has studied the beta-glucuronidase activity in several tissues such as liver, stomach and small intestine of the male and female rabbits infected with different doses of metacercariae of clonorchis sinensis. the metacercariae of clonorchis sinensis were isolated from pseudorasbora parva caught in kim hae by digestion technic. the experimental animals were sacrificed in the period of 1, 7, 14, 21, 28 and 35th days following the infection. the results obtained were summarized as follows ... | 1986 | 12886090 |
| purification of cystic fluid antigen of taenia solium metacestodes by affinity chromatography using monoclonal antibody and its antigenic characterization. | this study was undertaken to purify cystic fluid (cf) antigen of taenia solium metacestodes by affinity chromatography using specific monoclonal antibody (mcab) and to characterize the antigenicity of the purified antigen. the hybridoma cell lines, prepared by fusion between mouse plasmacytoma and spleen cells from balb/c mice immunized with cf, secrected antibodies reacting to various helminthic antigens. majority of cell lines reacted to cf only but some also reacted to parenchymal antigen of ... | 1986 | 12886091 |
| [intestinal parasite survey in seoul by stool examination at hanyang university hospital] | the present study was undertaken to evaluate the present status of intestinal parasitic infection in seoul area, korea. during the period from june 1985 to july 1986, a total of 5,251 stool samples were collected in department of clinical pathology, hanyang university hospital and examined by formalin-ether sedimentation technique once for helminth ova and protozoan cysts. the results were summerized as follows: the overall egg positive rate of intestinal helminthes was 2.53 per cent; and 1.43 p ... | 1986 | 12886099 |
| [a survey on intestinal parasites of soldiers in korea] | total of 2,643 korean soldiers were examined of their stool for parasitic infections by both cellophane thick smear and formalin-ether concentration techniques from august 1983 to december 1985. out of them, 73.6% were free from any parasite, 22.6% were ova positive and 4.0% cyst positive. the ova positive rates by species were ascaris lumbricoides 2.0%, trichuris trichiura 13.0%, hookworm and trichostrongylus orientalis 0.08% respectively, clonorchis sinensis 7.6%, metagonimus yokogawai 1.1%, p ... | 1986 | 12886100 |
| ten human cases of fibricola seoulensis infection and mixed one with stellantchasmus and metagonimus. | ten soldiers of the korean army were found to have f. seoulensis ova in their faeces. these men were treated with praziquantel and the worms passed after purging were successfully collected in one case: 59 f. seoulensis, 68 metagonimus and 2 stellantchasmus falcatus were collected. 2 men were also infected with clonorchis sinensis. all the cases of fibricoliasis had eaten raw or undercooked snake meat or frogs during survival training. all were egg-negative 2-6 months after treatment. | 1986 | 12886114 |
| [experimental studies on the second intermediate hosts of clonorchis sinensis iv. observations on the fate of metacercariae of clonorchis sinensis in the fish host, cultriculus eigenmanni] | in order to judge appropriately the suitability as the second intermediate host of clonorchis sinensis, the present experiments were proposed to survey the metacercaria of c. sinensis in the fish host, cultriculus eigenmanni, in the field, and to observe, by experimental infection of the fish with clonorchis cercaria, the penetrating ability of the cercaria, maturity, the process of degeneration and extinction, and infectivity of the metacercaria. the following is a brief summary of the leading ... | 1985 | 12888689 |
| seasonal variations of metacercarial density of clonorchis sinensis in fish intermediate host, pseudorasbora parva. | the seasonal variations of the rate and intensity of metacercarial infection of c. sinensis in p. parva were observed. the fish were collected at sun-am river which located in kim-hae city, kyong-sang-nam do(=province), korea, from march 1983 to february 1984 every month. a total of 788 fish was examined. the number of metacercariae in each fish was individually counted after the individual digestion by artificial gastric juice. the result was as follows: during one year, 513(65.1%) out of 788 f ... | 1985 | 12888690 |
| comparative morphology of eggs of heterophyids and clonorchis sinensis causing human infections in korea. | in order to provide some clues for differential diagnosis of trematode infections in fecal examination, the comparative morphology of eggs of 5 kinds of heterophyid flukes (metagonimus yokogawai, heterophyes heterophyes nocens, heterophyopsis continua, stellantchasmus falcatus and pygidiopsis summa) and clonorchis sinensis was studied. the eggs were obtained from distal portion of uteri of worms which were recovered from men after treatment. the characteristic shape and apperance of each kind of ... | 1984 | 12891010 |
| [survey on encysted cercaria of trematodes from fresh-water fishes in tongjin riverside areas in korea] | in an attempt to clarify the epidemiological feature of distomiasis in tongjin riverside area, the prevalence of distomiasis in the residents and infection rates of the metacercariae in fresh-water fishes were investigated at the upper, middle and lower reaches of the river from january to april 1984. the results obtained were summarized as follows: out of a total of 931 fresh-water fishes which composed of 33 different species, 611 fishes (65.6 %) of 31 species were found positive with digeneti ... | 1984 | 12891012 |
| [prevalence of intestinal helminthic infections and skin tests for paragonimus and clonorchis in tuberculosis patients] | this study was conducted to evaluate prevalence of intestinal helminthic infections and skin tests and paragonimus and clonorchis in tuberculosis(tb) patients in korea. stool examinations by formalin-ether technique and intradermal tests were carried out in 2 areas; masan tb hospital and kong-ju tb hospital. 1. the positive rate of intestinal helminths of any kind was 51.7 % among 329 patients in masan hospital, but 18.1 % among 215 patients in kong-ju hospital. 2. the infection rates of trichoc ... | 1984 | 12891014 |
| [a study on the fine structure of clonorchus sinensis, a liver fluke v. the mature spermatozoa] | an ultrastructural study on the mature spermatozoa of clonorchis sinensis was carried out. for this study, the liver flukes were collected from the livers of rabbits and rats artificially infected with the metacercariae obtained from the fresh water fish, pseudorasbora parva. six-month old worms were used. the collected liver flukes were washed with 0.85 percent saline solution and then immediately moved to cold 2 percent glutaraldehyde buffered with 0.l m millonig's phosphate buffer (ph 7.4). t ... | 1984 | 12891028 |
| studies on intestinal trematodes in korea xi. two cases of human infection by heterophyes heterophyes nocens. | two human cases of heterophyes heterophyes nocens infection were proved by identifying adult worms after treatment with bithionol or praziquantel in 1983 in korea. they are 37 (case 1) and 24-year old (case 2) males whose residence or native village is a southern coastal area in kohung-gun, chollanam-do. the case 1 had the gastrointestinal symptoms such as epigastric pain and indigestion, and the case 2 heart problems such as arrhythmia and ventricular premature beat in ekg and digestive symptom ... | 1984 | 12891029 |
| [infestation of larval trematodes from fresh-water fish and brackish-water fish in river hyungsan, kyungpook province, korea] | in order to determine the infestation patterns for metacercaria of digenetic trematodes, several kinds of fresh-water and brackish-water fish were caught in river hyungsan, kyungpook province, korea and examined from march to october in 1983. they were collected by netting, fishig with rod and line, or using the bait of crushed oil cake in transparent plastic bowls, and were dissected into the flesh, scales and fins with tail, and then examined for the presence and infestation density of larval ... | 1984 | 12891035 |
| recent patterns of intestinal helminth infections among the residents in taegu city, korea. | in order to estimate the recent pattens of intestinal helminth infections among the residents in taege city, korea, a survey based on discovery of helminth eggs by formalin-ether sedimentation and modified graham's scotch-tape anal swab techniques, were performed during the period from march to september in 1983. a total of 1,697 fecal specimens were collected from 854 male and 843 female residents from 5 villages(dong), each of whom were selected randomly to represent 1,000 persons referred to ... | 1984 | 12891040 |
| [the wormicidal substance of fresh water fishes on clonorchis sinensis v. purification and chemical characterization of clonorchicidal substance from epidermal mucus of cyprinus carpio] | as a series of studies to clarify clonorchicidal substances in body surface mucus of some fresh-water fishes, the substance in the epidermal mucus of cyprinus carpio was isolated by silica gel column and thin layer chromatography and analyzed for its chemical nature. wormicidal trial was done in vitro, and the results obtained are summarized as follows: the mucus was extracted by ethyl ether and separated into 4 fractions by column chromatography using benzene as solvent. the second fraction wit ... | 1984 | 12891042 |
| cytokine responses in mice infected with clonorchis sinensis. | fvb and balb/c mice show different morbidity, development of clonorchis sinensis, and pathological changes following c. sinensis infection. fvb mice are susceptible and balb/c mice are relatively more resistant to c. sinensis infection. to investigate the relationship between cytokine reaction and susceptibility to c. sinensis infection in fvb and balb/c mice, we described both the patterns and kinetics of th1 cytokines and th2 cytokines in spleen cell culture. interleukin (il)-4 and il-10 cytok ... | 2003 | 12898229 |
| the current pathobiology and chemotherapy of clonorchiasis. | this special issue is devoted to a review of the current state of knowledge of clonorchiasis. sections deal with: the biology of clonorchis sinensis; geographical distribution, with particular regard to korea; epidemiology; pathogenesis; clinical features; diagnosis; prevention and control; chemotherapy. | 1986 | 12902642 |