application of flow cytometry for the assessment of preservation and recovery efficiency of bioaerosol samplers spiked with pantoea agglomerans. | exposure assessment of biological aerosols requires trade-offs between efficient sampling of airborne microorganisms as either particles or viable units. the main objective of this work was to characterize aspects of bioaerosol measurement efficiency. a known concentration of the vegetative bacteria pantoea agglomerans was spiked onto different samplers (agi-30, biosampler, and membrane filters) and then run for increasing time periods using hepa filtered air. measurement efficiency was evaluate ... | 2007 | 17438801 |
pantoea agglomerans, a plant pathogen causing human disease. | we present 53 pediatric cases of pantoea agglomerans infections cultured from normally sterile sites in patients seen at a children's hospital over 6 years. isolates included 23 from the bloodstream, 14 from abscesses, 10 from joints/bones, 4 from the urinary tract, and 1 each from the peritoneum and the thorax. p. agglomerans was most associated with penetrating trauma by vegetative material and catheter-related bacteremia. | 2007 | 17442803 |
periodontal disease as reservoir for multi-resistant and hydrolytic enterobacterial species. | this investigation aimed to isolate enteric rods from subgingival sites of patients presenting chronic periodontitis lesions, and to assess antimicrobial resistance and expression of hydrolytic enzymes. | 2007 | 17451514 |
relationships of plant pathogenic enterobacteria based on partial atpd, cara, and reca as individual and concatenated nucleotide and peptide sequences. | relationships of the genera in the enterobacteriaceae containing plant pathogenic species: brenneria, dickeya, enterobacter, erwinia, pantoea, pectobacterium, and samsonia, were investigated by comparison of their nucleotide and peptide sequences of atpd, cara, reca, and the concatenated sequences. erwinia spp. and pantoea spp., with pectobacterium cypripedii, formed a group distinct from other pathogenic taxa. pectobacterium, brenneria, dickeya, and samsonia formed a contiguous clade. samsonia ... | 2007 | 17451899 |
pantoea agglomerans septicemia in three newborn infants. | pantoea infections are rare in humans, especially in neonates. infections are usually associated with plant thorn injury or outbreaks traced to contaminated parenteral nutrition, intravenous anesthetics or packed erythrocytes. between 1st of january 1994 and 1st of june 2005, 125 of 6383 patients (2%) in a 24-bed level iii nicu became colonized with pantoea agglomerans. three newborns exhibited late-onset pantoea agglomerans septicemia and died. sporadic cases of pantoea agglomerans septicemia h ... | 2007 | 17468662 |
acid tolerance response induced in the biocontrol agent pantoea agglomerans cpa-2 and effect on its survival ability in acidic environments. | the aim of this work was to optimize acid stress conditions for induction of acid tolerance response (atr) in the biocontrol agent pantoea agglomerans and study the effect of atr induced on the ability to survive under acidic conditions. initially, pantoea agglomerans was grown in mild acidic conditions (ph 6.0, 5.5, 5.0 and 4.0) in order to induce atr. the highest atr was induced at initial ph of 5 using malic or citric acid. a first in vitro experiment was carried out. thus, basal liquid mediu ... | 2009 | 17475457 |
the bryophyte genus sphagnum is a reservoir for powerful and extraordinary antagonists and potentially facultative human pathogens. | sphagnum plants grow in natural, species-poor carpets at low ph but without any known substantial fungal disease. to investigate this phenomenon, we analysed bacterial populations associated with two sphagnum species with different ecological behaviour, namely s. magellanicum and s. fallax, from three sites in germany and three in norway, with a special focus on the functional group of antagonists. the screening of 493 bacterial isolates for antagonistic activity against fungal pathogens resulte ... | 2007 | 17484734 |
increased beta-carotene production in recombinant escherichia coli harboring an engineered isoprenoid precursor pathway with mevalonate addition. | when pt-lycm4 containing lycopene synthetic genes was co-transformed with psucrty or pshcrty containing crty gene of pantoea ananatis (p. ananatis) or pantoea agglomerans (p. agglomerans), beta-carotene productions of 36 and 35 mg/l were obtained, respectively. no lycopene was detected in the beta-carotene production culture. pt-hb, constructed by addition of p. ananatis crty gene into pt-lycm4, was used for co-transformation with psdxs and pssn12didi, which increased isopentenyl diphosphate and ... | 2007 | 17500531 |
characterization of the cultivable microbiota of sprouts and their potential for application as protective cultures. | the microbiota of ten seeds and ready-to-eat sprouts produced thereof was characterized by bacteriological culture and denaturing gradient gel electrophoresis (dgge) of amplified dna fragments of the 16s rrna gene. the predominant bacterial biota of hydroponically grown sprouts mainly consisted of enterobacteria, pseudomonades and lactic acid bacteria (lab). for adzuki, alfalfa, mung bean, radish, sesame and wheat, the ratio of these bacterial groups changed strongly in the course of germination ... | 2007 | 17512686 |
bacterial reduction of selenate to elemental selenium utilizing molasses as a carbon source. | selecting an inexpensive and effective organic carbon source is the key to reducing the cost in selenium (se) remediation. five bacteria were screened based on their ability in using molasses as an organic carbon source to reduce selenate [se(vi)] in drainage water. efficiency of se removal differed in the molasses-added drainage water containing different bacteria, with an order of enterobacter taylorae>pantoea sp. sss2>klebsiella sp. wrs2>citerobacter freundii>shigella sp. dw2. by using e. tay ... | 2008 | 17512724 |
papaya shoot tip associated endophytic bacteria isolated from in vitro cultures and host-endophyte interaction in vitro and in vivo. | fourteen distinct bacterial clones were isolated from surface-sterilized shoot tips (approximately 1 cm) of papaya (carica papaya l. 'surya') planted on murashige and skoog (ms)-based papaya culture medium (23/50 nos.) during the 2-4 week period following in vitro culturing. these isolates were ascribed to six gram-negative genera, namely pantoea (p. ananatis), enterobacter (e. cloacae), brevundimonas (b. aurantiaca), sphingomonas, methylobacterium (m. rhodesianum), and agrobacterium (a. tumefac ... | 2007 | 17538647 |
fulminant infection of the lower limb in a diabetic patient. | | 2007 | 17552407 |
[mechanism of the effect of lpsp on the production of anti-hbs in mice]. | to investigate the mechanism of the effect of avirulent pantoea agglomerans lipopolysaccharide (lpsp) as the adjuvant of hbsag on the production of anti-hbs in mice. | 2007 | 17553355 |
the potential perils of a visit to botanical gardens in a patient with a central intravascular device. | | 2007 | 17573153 |
relative importance of amino acids, glycine-betaine and ectoine synthesis in the biocontrol agent pantoea agglomerans cpa-2 in response to osmotic, acidic and heat stress. | the objective of this work was to determine the role of different compatible solutes in adaptation of pantoea agglomerans cpa-2 at different stages of growth to solute (0.98, 0.97, 0.96 aw), heat (35 and 40 degrees c) and acidic (ph 4.0, 5.0, 6.0) stress. | 2007 | 17594453 |
bacteremia caused by pantoea agglomerans and enterococcus faecalis in a patient with colon cancer. | a case of bacteremia caused by pantoea agglomerans (p. agglomerans) and enterococcus faecalis in a male patient with colon cancer is reported. he was successfully treated with vancomycin and meropenem. the clinical isolate was identified as p. agglomerans by 16s rrna gene sequencing. | 2007 | 17600887 |
stability of microbial communities in goat milk during a lactation year: molecular approaches. | the microbial communities in milks from one herd were evaluated during 1-year of lactation, using molecular methods to evaluate their stability and the effect of breeding conditions on their composition. the diversity of microbial communities was measured using two approaches: molecular identification by 16s and 18s rdna sequencing of isolates from counting media (two milks), and direct identification using 16s rdna from clone libraries (six milks). the stability of these communities was evaluat ... | 2007 | 17604934 |
nosocomial outbreak of pantoea agglomerans in a pediatric urgent care center. | pantoea agglomerans is a gram-negative bacterium whose isolates can be found in blood cultures and other secretions. this article described one outbreak of sepsis due to this bacterium in the pediatric urgent care center of a tertiary hospital, in brasilia, federal district. this was a case-control study and it evaluated the risk factors for acquisition of nosocomial sepsis caused by pantoea agglomerans. six case-patients and 15 control-patients have been found. the risk factors were: vomiting i ... | 2007 | 17625778 |
transmission of cotton seed and boll rotting bacteria by the southern green stink bug (nezara viridula l.). | to determine the ability of the southern green stink bug (sgsb) (nezara viridula l.) to transmit pantoea agglomerans into cotton (gossypium hirsutum) bolls. | 2007 | 17650204 |
bilateral tibial chronic osteomyelitis due to pantoea agglomerans in a patient with sickle cell disease. | | 2007 | 17650525 |
recovery of pseudomonas savastanoi pv. savastanoi from symptomless shoots of naturally infected olive trees. | seasonal dynamics of pseudomonas savastanoi pv. savastanoi (psv) on stems and leaves from symptomless shoots of naturally infected olive trees was monitored in spanish olive orchards. data inferred from the comparison between washing of leaves and dilution-plating versus leaf printing of individual leaves suggested that psv population sizes varied by over several orders of magnitude, among leaves sampled concurrently from the same shoot. we did not find significant differences between leaves and ... | 2007 | 17661284 |
isolation of bacterial endophytes from germinated maize kernels. | the germination of surface-sterilized maize kernels under aseptic conditions proved to be a suitable method for isolation of kernel-associated bacterial endophytes. bacterial strains identified by partial 16s rrna gene sequencing as pantoea sp., microbacterium sp., frigoribacterium sp., bacillus sp., paenibacillus sp., and sphingomonas sp. were isolated from kernels of 4 different maize cultivars. genus pantoea was associated with a specific maize cultivar. the kernels of this cultivar were ofte ... | 2007 | 17668041 |
[effects of a lactic acid bacteria community sfc-2 treated on rice straw]. | aimed to utilize rice straw and lessen the pressure of environment, the rice straw was used as the fermentation material, and a lactic acid bacteria community sfc-2 from my laboratory was inoculated into the rice straw to investigate the inoculation effects. after 30 days fermentation, the inoculated fermented straw smelt acid-fragrant, and the ph value was 3.8, which was lower than the control of 4.1. furthermore, lactic acid concentration was more than that in the control. especially l-lactic ... | 2007 | 17674756 |
the plant pathogen pantoea ananatis produces n-acylhomoserine lactone and causes center rot disease of onion by quorum sensing. | a number of gram-negative bacteria have a quorum-sensing system and produce n-acyl-l-homoserine lactone (ahl) that they use them as a quorum-sensing signal molecule. pantoea ananatis is reported as a common colonist of wheat heads at ripening and causes center rot of onion. in this study, we demonstrated that p. ananatis sk-1 produced two ahls, n-hexanoyl-l-homoserine lactone (c6-hsl) and n-(3-oxohexanoyl)-l-homoserine lactone (3-oxo-c6-hsl). we cloned the ahl-synthase gene (eani) and ahl-recept ... | 2007 | 17827290 |
detection and visualization of an exopolysaccharide produced by xylella fastidiosa in vitro and in planta. | many phytopathogenic bacteria, such as ralstonia solanacearum, pantoea stewartii, and xanthomonas campestris, produce exopolysaccharides (epss) that aid in virulence, colonization, and survival. eps can also contribute to host xylem vessel blockage. the genome of xylella fastidiosa, the causal agent of pierce's disease (pd) of grapevine, contains an operon that is strikingly similar to the x. campestris gum operon, which is responsible for the production of xanthan gum. based on this information ... | 2007 | 17827325 |
engineering the halophilic bacterium halomonas elongata to produce beta-carotene. | engineering halophilic bacteria to produce carotenoids is a subject of great scientific and commercial interest, as carotenoids are desirable products used as additives and colorants in the food industry, with beta-carotene the most prominent. with this target, we expressed the beta-carotene biosynthetic genes crte, crty, crti, and crtb from pantoea agglomerans and the cdna encoding isopentenyl pyrophosphate isomerase from haematococcus pluvialis in the halophilic bacterium halomonas elongata ob ... | 2007 | 17899066 |
dry stress and survival time of enterobacter sakazakii and other enterobacteriaceae in dehydrated powdered infant formula. | powdered infant formula is not a sterile product, and opportunistic pathogens could multiply in the reconstituted product, resulting in neonatal infections. in this study, the generation of sublethally injured enterobacteriaceae during desiccation and their persistence in dehydrated powdered infant formula was assessed during a 2.5-year period. the study included 27 strains of enterobacter sakazakii, enterobacter cloacae, salmonella enteritidis, citrobacter koseri, citrobacter freundii, escheric ... | 2007 | 17900090 |
diversity of cultivated endophytic bacteria from sugarcane: genetic and biochemical characterization of burkholderia cepacia complex isolates. | bacteria were isolated from the rhizosphere and from inside the roots and stems of sugarcane plants grown in the field in brazil. endophytic bacteria were found in both the roots and the stems of sugarcane plants, with a significantly higher density in the roots. many of the cultivated endophytic bacteria were shown to produce the plant growth hormone indoleacetic acid, and this trait was more frequently found among bacteria from the stem. 16s rrna gene sequence analysis revealed that the select ... | 2007 | 17905875 |
the effect of micro-architectural structure of cabbage substratum and or background bacterial flora on the growth of listeria monocytogenes. | the effect of micro-architectural structure of cabbage (brassica oleracea var. capitata l.) substratum and or background bacterial flora on the growth of listeria monocytogenes as a function of incubation temperature was investigated. a cocktail mixture of pseudomonas fluorescens, pantoea agglomerans and lactobacillus plantarum was constituted to a population density of approximately 5 log cfu/ml in order to pseudo-simulate background bacterial flora of fresh-cut cabbage. this mixture was co-ino ... | 2007 | 17910986 |
distribution and replication of the pathogenicity plasmid ppath in diverse populations of the gall-forming bacterium pantoea agglomerans. | pantoea agglomerans has been transformed from a commensal bacterium into two related gall-forming pathovars by acquisition of ppath plasmids containing a pathogenicity island (pai). this pai harbors an hrp/hrc gene cluster, type iii effectors, and phytohormone biosynthetic genes. dna typing by pulsed-field gel electrophoresis revealed two major groups of p. agglomerans pv. gypsophilae and one group of p. agglomerans pv. betae. the ppath plasmids of the different groups had nearly identical repli ... | 2007 | 17921271 |
lidar detection algorithm for time and range anomalies. | a new detection algorithm for lidar applications has been developed. the detection is based on hyperspectral anomaly detection that is implemented for time anomaly where the question "is a target (aerosol cloud) present at range r within time t(1) to t(2)" is addressed, and for range anomaly where the question "is a target present at time t within ranges r(1) and r(2)" is addressed. a detection score significantly different in magnitude from the detection scores for background measurements sugge ... | 2007 | 17932542 |
[bacterial community structure in intestine of the white shrimp, litopenaeus vannamei]. | the composition of bacterial community in the intestine of the white shrimp, litopenaeus vannamei under laboratory culture condition was determined using the 16s rdna clone library. 16s rrna gene was amplified and a library was constructed by using the genomic dna extracted from the bacteria in the shrimp intestine as template. 12 different rflp patterns of the clones were obtained by restriction fragment length polymorphism analysis using afa i and msp i. compared with the published sequences i ... | 2007 | 17944366 |
development and potential use of a monoclonal antibody to the lipopolysaccharide of pantoea agglomerans (ip-pa1). | the lipopolysaccharide of pantoea agglomerans (ip-pa ) has been shown to be effective and safe in the prevention of various diseases, such as bacterial or viral infection, lifestyle-related diseases, when administered transdermally or orally. to clarify the mechanisms of the preventive or therapeutic effect induced by ip-pa1, we tried to establish a monoclonal antibody to detect ip-pa1. the enzyme-linked immunosorbent assay (elisa) was used to measure the amount of ip-pa1. | 2007 | 17970031 |
identification, antimicrobial resistance profiles, and virulence of members from the family enterobacteriaceae from the feces of yellow-headed blackbirds (xanthocephalus xanthocephalus) in north dakota. | public pressure to reduce or eliminate antimicrobials as ingredients of feed for poultry and other agricultural animals is mounting, primarily due to the fear of multidrug-resistant bacteria in clinical infections in both animals and humans. exploration of the occurrence of antibiotic resistance in the gut flora of wildlife avian flocks that presumptively do not receive antimicrobials will determine the rate of resistance in a naïve population. fecal samples collected from a healthy population o ... | 2007 | 17992921 |
virulence mechanisms and host specificity of gall-forming pantoea agglomerans. | pantoea agglomerans has been transformed from a commensal bacterium associated with many plants into a host-specific gall-forming pathogen by acquiring a plasmid-borne pathogenicity island. this pathogenicity island harbors the hrp/hrc gene cluster, in addition to genes encoding type iii effector proteins, biosynthesis of the phytohormones indole-3-acetic acid and cytokinin, multiple diverse insertion sequences and pseudogenes. this review describes a unique model for understanding the emergence ... | 2007 | 18024130 |
construction of stably maintained non-mobilizable derivatives of rsf1010 lacking all known elements essential for mobilization. | rsf1010 is a well-studied broad-host-range plasmid able to be mobilized to different bacteria and plants. rsf1010-derived plasmid vectors are widely used in both basic research and industrial applications. in the latter case, exploiting of mobilizable plasmids or even the plasmids possessing negligible mobilization frequency, but containing dna fragments that could promote conjugal transfer, is undesirable because of biosafety considerations. previously, several mutations significantly decreasin ... | 2007 | 18028554 |
the structure of the o-specific polysaccharide of the lipopolysaccharide from pantoea agglomerans strain fl1. | a neutral o-specific polysaccharide consisting of d-rhamnose was obtained by mild acid hydrolysis of the lipopolysaccharide of the plant pathogenic bacterium pantoea agglomerans strain fl1, a common epiphyte of many plant species, and associated with pseudomonas savastanoi pv. savastanoi in young and apparently intact olive knots. by means of compositional and methylation analyses, and nmr spectroscopy, the chemical repeating unit of the polymer was identified as a linear tetrasaccharide of the ... | 2008 | 18039532 |
two bacterial entophytes eliciting both plant growth promotion and plant defense on pepper (capsicum annuum l.). | plant growth-promoting rhizobacteria (pgpr) have the potential to be used as microbial inoculants to reduce disease incidence and severity and to increase crop yield. some of the pgpr have been reported to be able to enter plant tissues and establish endophytic populations. here, we demonstrated an approach to screen bacterial endophytes that have the capacity to promote the growth of pepper seedlings and protect pepper plants against a bacterial pathogen. initially, out of 150 bacterial isolate ... | 2007 | 18051359 |
diverse endophytic nitrogen-fixing bacteria isolated from wild rice oryza rufipogon and description of phytobacter diazotrophicus gen. nov. sp. nov. | twenty-three nitrogen-fixing bacteria were isolated from surface-sterilized stems and roots of wild rice oryza rufipogon. four clusters were defined among these bacteria by sds-page protein patterns and further confirmed by is-pcr finger-printing analysis. phylogenetic analysis of 16s rrna gene sequences showed that the representative strains ls 8 and ls 18 of cluster ii formed a monophyletic group sharing 94.0-97.3% similarities with defined enterobacterial species within the genera salmonella, ... | 2008 | 18060384 |
identification of an alternative translation initiation site for the pantoea ananatis lycopene cyclase (crty) gene in e. coli and its evolutionary conservation. | previous sequence analyses of the lycopene cyclase gene (crt y) from pantoea ananatis revealed that translation of its protein product in escherichia coli began at the atg start codon. we found, however, that this enzyme could also be produced in e. coli without the atg start codon present. results of experiments using crt y mutants revealed that a gtg (val) sequence, located in-frame and 24 bp downstream of the atg, could act as a potential start codon. additionally, a point-mutated gta (val), ... | 2008 | 18096401 |
pantoea agglomerans bacteremia in a 65-year-old man with acute myeloid leukemia: case report and review. | a 65-year-old man with a recent history of acute leukemia was admitted with complaints of chills and rigors. he had a long-standing hickman catheter which was removed following the isolation of a gram negative rod later identified as pantoea agglomerans. the patient recovered with antimicrobial therapy. pantoea species are rare causes of clinically relevant infections. | 2008 | 18176303 |
rapid detection and identification of the bacterium pantoea stewartii in maize by taqman real-time pcr assay targeting the cpsd gene. | the development and evaluation of a sensitive and specific taqman real-time polymerase chain reaction (pcr) for the detection and identification of pantoea stewartii on maize. | 2008 | 18179542 |
transgenic tobacco revealing altered bacterial diversity in the rhizosphere during early plant development. | the rhizosphere constitutes a complex niche that may be exploited by a wide variety of bacteria. bacterium-plant interactions in this niche can be influenced by factors such as the expression of heterologous genes in the plant. the objective of this work was to describe the bacterial communities associated with the rhizosphere and rhizoplane regions of tobacco plants, and to compare communities from transgenic tobacco lines (cab1, cab2 and trp) with those found in wild-type (wt) plants. samples ... | 2008 | 18181027 |
effect of biocontrol agents candida sake and pantoea agglomerans on penicillium expansum growth and patulin accumulation in apples. | penicillium expansum is the major responsible of fruit pome decaying in cold storage. apples spoiled by p. expansum are expected to contain patulin, a mycotoxin which is proven to affect human health. the use of chemicals is the most common procedure to prevent rots in postharvest but legislation is becoming more and more restrictive. the use of biocontrol agents (bca) as an alternative tool is currently being proposed. the aim of this study was to evaluate the effect of two bca (candida sake cp ... | 2008 | 18191492 |
extracellular polysaccharides produced by cooling water tower biofilm bacteria and their possible degradation. | the extracellular polymers (eps) of biofilm bacteria that can cause heat and mass transfer problems in cooling water towers in the petrochemical industry were investigated. in addition, these microorganisms were screened for their ability to grow and degrade their own eps and the eps of other species. twelve bacteria producing the most eps were isolated from cooling water towers and characterized biochemically by classic and commercial systems. these were species of pseudomonas, burkholderia, ae ... | 2008 | 18256966 |
16s rrna gene sequences from bacteria associated with adult anopheles darlingi (diptera: culicidae) mosquitoes. | the microbial flora associated with anopheles darlingi root (diptera: culicidae), a major neotropical malaria vector, was investigated for the development of a paratransgenesis-based approach to control malaria transmission in brazil. female mosquitoes were collected using human land catches and captured insects provided a bloodmeal. the controlled blood feeding resulted in increased detection of mosquito bacterial population because it was possible to retrieve bacterial dna from all blood-fed m ... | 2008 | 18283961 |
taxonomic structure and stability of the bacterial community in belgian sourdough ecosystems as assessed by culture and population fingerprinting. | a total of 39 traditional sourdoughs were sampled at 11 bakeries located throughout belgium which were visited twice with a 1-year interval. the taxonomic structure and stability of the bacterial communities occurring in these traditional sourdoughs were assessed using both culture-dependent and culture-independent methods. a total of 1,194 potential lactic acid bacterium (lab) isolates were tentatively grouped and identified by repetitive element sequence-based pcr, followed by sequence-based i ... | 2008 | 18310426 |
cold atmospheric plasma decontamination of the pericarps of fruit. | this investigation describes the inactivation by cold atmospheric plasmas of one pathogenic and three spoilage organisms on the pericarps of mangoes and melons. the operating voltage necessary for efficient microbial decontamination of fruit pericarps was first established using escherichia coli at a concentration of 10(7) cfu/cm2 on the surface of mango. it was found that, when the plasma was sustained slightly above its breakdown voltage of 12 kv (peak to peak), no inactivation was detected wh ... | 2008 | 18326179 |
severe peritonitis due to pantoea agglomerans in a ccpd patient. | | 2008 | 18332463 |
bacillus spore classification via surface-enhanced raman spectroscopy and principal component analysis. | surface-enhanced raman spectroscopy (sers) can provide rapid fingerprinting of biomaterial in a nondestructive manner. the adsorption of colloidal silver to biological material suppresses native biofluorescence while providing electromagnetic surface enhancement of the normal raman signal. this work validates the applicability of qualitative ser spectroscopy for analysis of bacterial species by utilizing principal component analysis (pca) to show discrimination of biological threat simulants, ba ... | 2008 | 18339232 |
stabilization of psw100 from pantoea stewartii by the f conjugation system. | plasmid psw100 is 1 of the 13 plasmids from pantoea stewartii subsp. stewartii sw2 which has a replicon that resembles that of cole1. this work uses a psw100 derivative, psw140k, to study how the psw100 replicon is stably maintained in its hosts. our results indicate that although psw140k is stable in escherichia coli hb101, the plasmid is rapidly lost in another e. coli strain, dh5alpha, indicating that the genetic background of an e. coli strain affects the stability of psw140k. mutagenesis of ... | 2008 | 18344358 |
bacteria associated with ectomycorrhizas of slash pine (pinus elliottii) in south-eastern queensland, australia. | bacterial communities associated with ectomycorrhizal and uncolonized roots of pinus elliottii (slash pine) collected from a plantation in south-east queensland, australia, were investigated, using cultivation-dependent and -independent methods. denaturing gradient gel electrophoresis (dgge) analysis of 16s rrna gene pcr products obtained using a cultivation-independent approach revealed that bacterial communities associated with ectomycorrhizal root tips differed significantly from those associ ... | 2008 | 18355286 |
[study of ectoparasitism of ultramicrobacteria of the genus kaistia, strains nf1 and nf3 by electron and fluorescence microscopy]. | transmission electron and fluorescence microscopy was used to study the character of the interaction of free-living ultramicrobacterial (umb) strains nf1 and nf3, affiliated with the genus kaistia, and seven species of gram-positive and gram-negative heterotrophic bacteria. strains nf1 and nf3 were found to exhibit parasitic activity against gram-positive bacillus subtilis and gram-negative acidovorax delafildii. umb cells are tightly attached to the envelopes of the victim cells and induce thei ... | 2008 | 18365722 |
genetic diversity of endophytic bacteria which could be find in the apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants. | the genetic diversity of 29 endophytic bacterial strains isolated from apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants grown in cuba was analysed by two primers-ramdom amplified polymorphic dna fingerprinting (tp-rapd) and 16s rrna gene sequencing. the strains were distributed into 17 groups on the basis of their tp-rapd patterns, and a representative strain from each group was subjected to 16s rrna gene sequencing. analysis of these sequences showed that the is ... | 2008 | 18383223 |
potential sources of microbial contamination of satsuma mandarin fruit in japan, from production through packing shed. | potential sources of microbial contamination of satsuma mandarin fruit were investigated from production through the packing shed in the 2005 season. microbial counts in the peel and flesh during the fruit development stage were below 2.4 log cfu/g for bacteria and 3 log cfu/g for fungi, except for the peel in august and september. in the field environment, the highest microbial counts were found in fallen leaves on the ground, followed by soil, organic fertilizer, and agricultural water. only t ... | 2008 | 18389696 |
growth-inhibitory effects of sulfonamides at different ph: dissimilar susceptibility patterns of a soil bacterium and a test bacterium used for antibiotic assays. | the ionic speciation of sulfonamides is ph-driven and this may be crucial for their bioavailability and sorption to soil constituents, as well as for their uptake into bacterial cells. the inhibition behaviour of a bacterial test strain (pseudomonas aeruginosa; dsm 1117), which was grown in the presence of different concentrations of 8 sulfonamides at ph values from 5 to 8, could be predicted by models that take the speciation of sulfonamides in- and outside of bacterial cells into account. assu ... | 2008 | 18396316 |
evaluation of antifungal activity of carbonate and bicarbonate salts alone or in combination with biocontrol agents in control of citrus green mold. | the aim of this research was to determine if the attacks of green mold on orange could be reduced by edible salts alone or in combination with biocontrol agent. for this purpose toxicity to pantoea digitatum and practical use of sodium carbonate (sc), sodium bicarbonate (sbc) and potassium carbonate, and potassium bicarbonate alone or in combination with antagonistic bacteria (pseudomonas fluorescens isolate pn, bacillus subtilis isolate vhn, pantoea agglomerans isolate ca) to control green mold ... | 2007 | 18396809 |
dgge and real-time pcr analysis of lactic acid bacteria in bacterial communities of the phyllosphere of lettuce. | food associated indigenous microbial communities exert antagonistic effects on pathogens and may routinely deliver health relevant microorganisms to the gi tract. by using molecular, culture independent methods including pcr-dgge of 16s rdna-coding regions and real-time pcr (rt-pcr) as well as biolog metabolic fingerprinting, microbial communities on lettuce were analyzed in samples from fields, from supermarkets and soil. amplified 16s rrna gene sequences (57.7%) could be assigned to species pr ... | 2008 | 18398868 |
absolute configuration and antimicrobial activity of acylhomoserine lactones. | (s)-n-heptanoylhomoserine lactone is an uncommon acyl odd-chain natural product employed by many gram-negative bacteria as a signaling substance in chemical communication mechanisms known as quorum sensing. the absolute configuration determination of the metabolite produced by the phytopathogen pantoea ananatis serrano is reported herein. as with all other substances of this class, the lactone moiety possesses s configuration, corroborating the hypothesis that it shares the same biosynthetic pat ... | 2008 | 18465897 |
response of endophytic bacterial communities in banana tissue culture plantlets to fusarium wilt pathogen infection. | endophytic bacteria reside within plant hosts without having pathogenic effects, and various endophytes have been found to functionally benefit plant disease suppressive ability. in this study, the influence of banana plant stress on the endophytic bacterial communities, which was achieved by infection with the wilt pathogen fusarium oxysporum f. sp. cubense, was examined by cultivation-independent denaturing gradient gel electrophoresis analysis of 16s ribosomal dna directly amplified from plan ... | 2008 | 18497482 |
is 16s rdna a reliable phylogenetic marker to characterize relationships below the family level in the enterobacteriaceae? | the phylogenetic relationships of multiple enterobacterial species were reconstructed based on 16s rdna gene sequences to evaluate the robustness of this housekeeping gene in the taxonomic placement of the enteric plant pathogens erwinia, brenneria, pectobacterium, and pantoea. four data sets were compiled, two of which consisted of previously published data. the data sets were designed in order to evaluate how 16s rdna gene phylogenies are affected by the use of different plant pathogen accessi ... | 2008 | 18504519 |
ribosomal rna-based analysis of the bacterial flora from the conjunctivae of cattle with bovine keratoconjunctivitis (bkc). | bovine keratoconjunctivitis (bkc), colloquially referred to as 'pinkeye', is a disease affecting cattle worldwide; it costs cattle producers millions of dollars in economic loss annually. while moraxella spp. are the primary etiologic agent of pinkeye, surveys of flora from the conjunctivae of livestock from around the world have indicated that a variety of bacterial commensals occupy this niche. we used molecular biology-based methods to determine the composition of bacterial flora in the conju ... | 2008 | 18513895 |
short peptide induces an "uncultivable" microorganism to grow in vitro. | microorganisms comprise the bulk of biodiversity, but only a small fraction of this diversity grows on artificial media. this phenomenon was noticed almost a century ago, repeatedly confirmed, and termed the "great plate count anomaly." advances in microbial cultivation improved microbial recovery but failed to explain why most microbial species do not grow in vitro. here we show that at least some of such species can form domesticated variants capable of growth on artificial media. we also pres ... | 2008 | 18515474 |
pantoea agglomerans: an unusual inciting agent in peritonitis. | | 2008 | 18556394 |
pantoea peritonitis: not just a "thorny" problem. | | 2008 | 18556396 |
in vitro study of pro-inflammatory and anti-tumour properties of microvesicles from bacterial cell wall of pantoea agglomerans. | in the environment, gram-negative bacteria are capable of producing large amounts of endotoxin-containing microvesicles - spherical structures measuring 30-50 nm in diameter, emerging by fragmentation of the outer membrane of the bacterial cell wall. microvesicles are suspected of inducing inflammatory lung diseases, but possibly also of stimulating anti-tumour defence mechanisms. the present study was aimed at assessing the pro-inflammatory and anti-tumour properties of microvesicles in vitro. ... | 2008 | 18581995 |
new erwinia-like organism causing cervical lymphadenitis. | the first case of cervical lymphadenitis due to infection by a new erwinia-like organism is reported. the organism was identified initially as pantoea sp. by a vitek 2-based assessment but was finally identified as a member of the genus erwinia by 16s rrna gene sequence analysis. the isolate displayed 98.9% 16s rrna gene sequence similarity to that of e. tasmaniensis and showed phenotypic characteristics that were different from other erwinia species. | 2008 | 18614665 |
quorum-sensing system affects gall development incited by pantoea agglomerans pv. gypsophilae. | the quorum-sensing (qs) regulatory system of the gall-forming pantoea agglomerans pv. gypsophilae was identified. mass spectral analysis, together with signal-specific biosensors, demonstrated that p. agglomerans pv. gypsophilae produced n-butanoyl-l-homoserine lactone (c4-hsl) as a major and n-hexanoyl-l-homoserine lactone (c6-hsl) as a minor qs signal. homologs of luxi and luxr regulatory genes, pagi and pagr, were characterized in strain p. agglomerans pv. gypsophilae pag824-1 and shown to be ... | 2008 | 18616406 |
the product chain length determination mechanism of type ii geranylgeranyl diphosphate synthase requires subunit interaction. | the product chain length determination mechanism of type ii geranylgeranyl diphosphate synthase from the bacterium, pantoea ananatis, was studied. in most types of short-chain (all-e) prenyl diphosphate synthases, bulky amino acids at the fourth and/or fifth positions upstream from the first aspartate-rich motif play a primary role in the product determination mechanism. however, type ii geranylgeranyl diphosphate synthase lacks such bulky amino acids at these positions. the second position upst ... | 2008 | 18616462 |
persistence of salmonella senftenberg in poultry production environments and investigation of its resistance to desiccation. | most salmonella serovars, including salmonella enterica subsp. enterica serovar senftenberg (s. senftenberg), are tolerant to desiccation and able to colonize and persist in feed mills. in addition, they may survive cleaning and disinfection procedures used on poultry farms. the present study was conducted to investigate the survival of s. senftenberg in broiler parent stock farms and broiler farms. the isolates from one of the parent stock farms investigated only differed by a single band in fl ... | 2008 | 18622860 |
bacteremia in equine neonatal diarrhea: a retrospective study (1990-2007). | bacteremia in sick foals is associated with survival, but the association of bacteremia and diarrhea is not reported. | 2008 | 18638014 |
enrichment of carotenoids in flaxseed (linum usitatissimum) by metabolic engineering with introduction of bacterial phytoene synthase gene crtb. | linseed flax (linum usitatissimum l.) is an industrially important oil crop, which includes large amounts of alpha-linolenic acid (18:3) and lignan in its seed oil. we report here the metabolic engineering of flax plants to increase carotenoid amount in seeds. agrobacterium-mediated transformation of flax was performed to express the phytoene synthase gene (crtb) derived from the soil bacterium pantoea ananatis (formerly called erwinia uredovora 20d3) under the control of the cauliflower mosaic ... | 2008 | 18640603 |
arsenite oxidation by alcaligenes sp. strain rs-19 isolated from arsenic-contaminated mines in the republic of korea. | arsenite [as(iii)]-oxidizing bacteria play important roles in reducing arsenic [as] toxicity and mobility in as-contaminated areas. as-resistant bacteria were isolated from the soils of two abandoned mines in the republic of korea. the isolated bacteria showed relatively high resistances to as(iii) up to 26 mm. the pcr-based 16s rrna analysis revealed that the isolated as-resistant bacteria were close relatives to serratia marcescensa, pseudomonas putida, pantoea agglomerans, and alcaligenes sp. ... | 2009 | 18642094 |
quorum-sensing signals in the microbial community of the cabbage white butterfly larval midgut. | the overall goal of this study was to examine the role of quorum-sensing (qs) signals in a multispecies microbial community. toward this aim, we studied qs signals produced by an indigenous member and an invading pathogen of the microbial community of the cabbage white butterfly (cwb) larval midgut (pieris rapae). as an initial step, we characterized the qs system in pantoea cwb304, which was isolated from the larval midgut. a luxi homolog, designated pani, is necessary for the production of n-a ... | 2008 | 18650927 |
comparative analysis of antibiotic resistance characteristics of gram-negative bacteria isolated from laying hens and eggs in conventional and organic keeping systems in bavaria, germany. | by investigating the prevalence and resistance characteristics of gram-negative bacteria from organic and conventional kept laying hens against 31 (campylobacter: 29) different antibiotics using the microdilution method, we determined to what extent different keeping systems influence bacterial resistance patterns. for this purpose, samples from 10 organic and 10 conventional flocks in bavaria (germany) were investigated four times between january 2004 and april 2005. altogether, 799 cloacal swa ... | 2008 | 18667026 |
transstadial and horizontal transfer of bacteria within a colony of anopheles gambiae (diptera: culicidae) and oviposition response to bacteria-containing water. | in a paratransgenic approach, genetically modified bacteria are utilized to kill the parasite in the vector gut. a critical component for paratransgenics against malaria is how transgenic bacteria can be introduced and then kept in a mosquito population. here, we investigated transstadial and horizontal transfer of bacteria within an anopheles gambiae mosquito colony with the focus on spiked breeding sites as a possible means of introducing bacteria to mosquitoes. a pantoea stewartii strain, pre ... | 2008 | 18671931 |
[intestinal enterobacteria of the hibernating apis mellifera mellifera l. bees]. | dynamics of enterobacteria of normal intestinal microflora was studied in apis mellifera mellifera l. bees hibernating under snow in the western urals. the cell numbers (n) of the predominant species klebsiella oxytoca increased from 10-10(6) cfu/bee in november 2004 to 10(4)-10(7) cfu/bee in march 2005; its frequency of occurrence (p) increased from 92 to 100%. increase of providencia rettgeri (11.2004: n up to 10(6), p 25%; 03.2005: n 10(2)-10(6), p 80%) was accompanied by the substitution of ... | 2008 | 18683661 |
diversity of endophytic bacteria from eucalyptus species seeds and colonization of seedlings by pantoea agglomerans. | the diversity and beneficial characteristics of endophytic microorganisms have been studied in several host plants. however, information regarding naturally occurring seed-associated endophytes and vertical transmission among different life-history stages of hosts is limited. endophytic bacteria were isolated from seeds and seedlings of 10 eucalyptus species and two hybrids. the results showed that endophytic bacteria, such as bacillus, enterococcus, paenibacillus and methylobacterium, are verti ... | 2008 | 18710397 |
diversity of endophytic enterobacteria associated with different host plants. | fifty-three endophytic enterobacteria isolates from citrus, cocoa, eucalyptus, soybean, and sugar cane were evaluated for susceptibility to the antibiotics ampicillin and kanamycin, and cellulase production. susceptibility was found on both tested antibiotics. however, in the case of ampicillin susceptibility changed according to the host plant, while all isolates were susceptible to kanamycin. cellulase production also changed according to host plants. the diversity of these isolates was estima ... | 2008 | 18758726 |
diversity of auxin-producing bacteria associated to pseudomonas savastanoi -induced olive knots. | forty three strains were isolated from knots induced by pseudomonas savastanoi in different olive cultivars. all the selected bacteria were shown to produce variable amounts of the plant growth hormone indole-3-acetic acid (iaa). amplification of the intergenic transcribed spacers (its) between 16s and 23s rdna genes, allowed the clustering of the isolates into seven distinct groups. all isolates from its group 1 were positive to the pseudomonas savastanoi pv. savastanoi specific iaa l gene as s ... | 2008 | 18759227 |
endophytic population of pantoea agglomerans in citrus plants and development of a cloning vector for endophytes. | harmless bacteria inhabiting inner plant tissues are termed endophytes. population fluctuations in the endophytic bacterium pantoea agglomerans associated with two species of field cultured citrus plants were monitored over a two-year period. the results demonstrated that populations of p. agglomerans fluctuated in citrus reticulata but not c. sinensis. a cryptic plasmid ppa3.0 (2.9 kb) was identified in 35 out of 44 endophytic isolates of p. agglomerans and was subsequently sequenced. the origi ... | 2008 | 18759238 |
andrimid producers encode an acetyl-coa carboxyltransferase subunit resistant to the action of the antibiotic. | andrimid is a hybrid nonribosomal peptide-polyketide antibiotic that blocks the carboxyl-transfer reaction of bacterial acetyl-coa carboxylase (acc) and thereby inhibits fatty acid biosynthesis with submicromolar potency. the andrimid biosynthetic gene cluster from pantoea agglomerans encodes an admt gene with homology to the acetyl-coa carboxyltransferase (ct) beta-subunit gene accd. escherichia coli cells overexpressing admt showed resistance to andrimid. co-overproduction of admt with e. coli ... | 2008 | 18768797 |
case report: subacute synovitis of the knee after a rose thorn injury: unusual clinical picture. | synovitis secondary to penetrating plant thorn injuries is not frequently reported. historically, it is considered aseptic and treated with removal of the intraarticular foreign body and affected synovial lining. we report a 57-year-old healthy man who was admitted 2 weeks after being injured by a rose (rosacea) thorn with subacute and mild synovitis with effusion of his right knee. no intraarticular foreign body was retained. pantoea agglomerans was identified in the synovial fluid. contrary to ... | 2008 | 18773251 |
in vitro adsorption revealing an apparent strong interaction between endophyte pantoea agglomerans ys19 and host rice. | pantoea (formerly enterobacter) agglomerans ys19 is a dominant diazotrophic endophyte isolated from rice (oryza sativa cv. yuefu) grown in a temperate-climate region in west beijing, china. in vitro adsorption and invasion of ys19 on host plant root were studied in this research. adsorption of ys19 on rice seedling roots closely resembled the langmuir adsorption and showed a higher adsorption quantity than the control strains paenibacillus polymyxa wy110 (a rhizospheric bacterium from the same r ... | 2008 | 18781359 |
pantoea stewartii subsp. stewartii exhibits surface motility, which is a critical aspect of stewart's wilt disease development on maize. | pantoea stewartii subsp. stewartii is a plant-pathogenic bacterium that causes stewart's vascular wilt in maize. the organism is taxonomically described as aflagellated and nonmotile. we recently showed that p. stewartii colonizes the xylem of maize as sessile, cell-wall-adherent biofilms. biofilm formation is a developmental process that generally involves some form of surface motility. for that reason, we reexamined the motility properties of p. stewartii dc283 based on the assumption that the ... | 2008 | 18785831 |
phylogenetic placement of pentatomid stink bug gut symbionts. | insect bacterial symbionts are ubiquitous, however, only a few groups of host families have been well studied in relation to their associations with microbes. the determination of the phylogenetic relationships among bacteria associated with different species within an insect family can provide insights into the biology and evolution of these interactions. we studied the phylogenetic placement of vertically transmitted bacterial symbionts associated with the posterior midgut (crypt-bearing) regi ... | 2009 | 18810535 |
biosynthesis of the vitamin e compound delta-tocotrienol in recombinant escherichia coli cells. | the biosynthesis of natural products in a fast growing and easy to manipulate heterologous host system, such as escherichia coli, is of increasing interest in biotechnology. this procedure allows the investigation of complex natural product biosynthesis and facilitates the engineering of pathways. here we describe the cloning and the heterologous expression of tocochromanol (vitamin e) biosynthesis genes in e. coli. tocochromanols are synthesized solely in photosynthetic organisms (cyanobacteria ... | 2008 | 18810749 |
pantoea agglomerans bloodstream infection in preterm neonates. | objective: to report an uncommon incidence of sporadic bloodstream infection (bsi) caused by pantoea agglomerans in preterm neonates. case presentation and intervention: fives cases of nosocomial bsi with p. agglomerans in preterm neonates (weight <or=1,500 g; age 8-17 days; gestational age 26-30 weeks) are presented. all cases were late onset neonatal sepsis (>7 days of age). lethargy, skin mottling and bradycardia were often present. although there was no evidence of pneumonia, desaturation wa ... | 2008 | 18836282 |
role of blossoms in watermelon seed infestation by acidovorax avenae subsp. citrulli. | abstract the role of watermelon blossom inoculation in seed infestation by acidovorax avenae subsp. citrulli was investigated. approximately 98% (84/87) of fruit developed from blossoms inoculated with 1 x 10(7) or 1 x 10(9) cfu of a. avenae subsp. citrulli per blossom were asymptomatic. using immunomagnetic separation and the polymerase chain reaction, a. avenae subsp. citrulli was detected in 44% of the seed lots assayed, despite the lack of fruit symptoms. furthermore, viable colonies were re ... | 2003 | 18942974 |
temperature and pomaceous flower age related to colonization by erwinia amylovora and antagonists. | abstract fire blight of apple and pear is initiated by epiphytic populations of erwinia amylovora on flower stigmas. predicting this disease and managing it with microbial antagonists depends on an understanding of bacterial colonization on stigmas. detached 'manchurian' crab apple flowers were inoculated with e. amylovora and subjected to a range of constant temperatures or various fluctuating temperature regimes. results may have application to disease risk assessment systems such as the couga ... | 2004 | 18943112 |
temporal dynamics of corn flea beetle populations infested with pantoea stewartii, causal agent of stewart's disease of corn. | abstract in order to better understand the epidemiology of the stewart's disease of corn pathosystem, quantitative information concerning the temporal dynamics of the amount of pathogen inoculum present in the form of pantoea stewartii-infested corn flea beetles (chaetocnema pulicaria) is needed. temporal changes in the proportion of p. stewartii-infested corn flea beetle populations were monitored by testing individual corn flea beetles for the presence of p. stewartii using a peroxidase-labele ... | 2003 | 18943136 |
bacterial populations associated with rice seed in the tropical environment. | abstract during the 1995 wet season, harvested rice seed was collected from farmers' fields at different locations in iloilo, philippines. bacterial isolations from crushed seed yielded 428 isolates. the isolates were characterized by box-polymerase chain reaction fingerprinting of total genomic dna and represented 151 fingerprint types (fpt). most fpts were found on a single occasion, although matching fingerprints for isolates from different samples also were found. identifications were made b ... | 2001 | 18943348 |
quantitative trait loci in sweet corn associated with partial resistance to stewart's wilt, northern corn leaf blight, and common rust. | abstract partial resistance to stewart's wilt (erwina stewartii, syn. pantoea stewartii), northern corn leaf blight (nclb) (exserohilum turcicum), and common rust (puccinia sorghi) was observed in an f(2:3) population developed from a cross between the inbred sweet corn lines il731a and w6786. the objective of this study was to identify quantitative trait loci (qtl) associated with partial resistance using restriction fragment length polymorphic markers. phenotypic data were collected for 2 year ... | 2001 | 18943349 |
antibiosis and acidification by pantoea agglomerans strain e325 may contribute to suppression of erwinia amylovora. | pantoea agglomerans strain e325, a commercially available antagonist for fire blight of apple and pear, was originally selected through screening based on suppression of erwinia amylovora on flower stigmas, but specific mechanisms of antagonism were unknown. bacterial modification of ph was evaluated as a possible mechanism by analyzing stigma exudates extracted from 'gala' apple stigmas. the ph values for field samples were only slightly lower than controls, but indicated a range (ph 5 to 6) co ... | 2008 | 18943460 |
use of fatty acid methyl ester profiles to compare copper-tolerant and copper-sensitive strains of pantoea ananatis. | abstract a survey was conducted to evaluate differences in fatty acid methyl ester (fame) profiles among strains of pantoea ananatis, causal agent of center rot of onion (allium cepa), isolated from 15 different onion cultivars in three different sites in georgia. differences in fame composition were determined by plotting principal components (pcs) in two-dimensional plots. euclidean distance squared (ed(2)) values indicated a high degree of similarity among strains. plotting of pcs calculated ... | 2007 | 18943688 |
comparative dynamics of adherent and nonadherent bacterial populations on maize leaves. | abstract the dynamics of the adherent and nonadherent populations of three bacterial species on maize leaves were examined to identify the extent to which bacteria adhere to leaves and the importance of this adhesion to leaf colonization. pantoea agglomerans strain brt98, clavibacter michiganensis subsp. nebraskensis strain gh2390, and pseudomonas syringae pv. syringae strain hs191r all rapidly adhered to maize leaves following inoculation, but differed in the percentage of cells that adhered to ... | 2002 | 18944027 |
selection for quantitative trait loci associated with resistance to stewart's wilt in sweet corn. | the objectives of this research were to identify quantitative trait loci (qtl) for stewart's wilt resistance from a mapping population derived from a sweet corn hybrid that is highly resistant to pantoea stewartii and to determine if marker-based selection for those qtl could substantially improve stewart's wilt resistance in a population derived from a cross of resistant lines and a highly susceptible sweet corn inbred. three significant qtl for stewart's wilt resistance on chromosomes 2 (bin 2 ... | 2008 | 18944197 |
antibiosis contributes to biological control of fire blight by pantoea agglomerans strain eh252 in orchards. | abstract fire blight, caused by erwinia amylovora, is the most serious bacterial disease of pear and apple trees. biological control with strains of pantoea agglomerans (syn. erwinia herbicola) may provide an effective disease management strategy for fire blight. most strains of p. agglomerans evaluated for suppression of fire blight produce compounds that inhibit the growth of e. amylovora in culture. the role of these inhibitory compounds in fire blight suppression in orchard environments has ... | 2002 | 18944246 |
assessment of environmental factors influencing growth and spread of pantoea agglomerans on and among blossoms of pear and apple. | abstract we evaluated effects of both physical and biological components of the environment on growth of pantoea agglomerans on inoculated pear and apple blossoms and on spread of the bacterium to blossoms on non-inoculated trees. the center three rows of 0.35- to 0.5-ha blocks of four pear cultivars and four apple cultivars were sprayed with a suspension of streptomycin-resistant p. agglomerans strain c9-1s (c9-1s) at 20 to 60% and 60 to 90% bloom. cultivars were chosen to create a sequence of ... | 2000 | 18944433 |
anatomy of cranberry stem gall and localization of bacteria in galls. | abstract cranberry stem gall is characterized by tumors that girdle stems, thereby killing all distal leaves, flowers, and fruit. bacteria that produce high levels of the plant growth hormone indole-3-acetic acid (iaa) are associated with and believed to cause cranberry stem gall. the anatomy of naturally occurring galls on woody cranberry plants and galls caused by inoculation of micropropagated cranberry plants with pantoea agglomerans strain 4/99 was consistent with elevated levels of iaa in ... | 2004 | 18944452 |