| phytase-producing capacity of yeasts isolated from traditional african fermented food products and phypk gene expression of pichia kudriavzevii strains. | phytate is known as a strong chelate of minerals causing their reduced uptake by the human intestine. ninety-three yeast isolates from traditional african fermented food products, belonging to nine species (pichia kudriavzevii, saccharomyces cerevisiae, clavispora lusitaniae, kluyveromyces marxianus, millerozyma farinosa, candida glabrata, wickerhamomyces anomalus, hanseniaspora guilliermondii and debaryomyces nepalensis) were screened for phytase production on solid and liquid media. 95% were a ... | 2015 | 25910031 |
| determination of the effect of nd:yag laser irradiation through dentinal tubules on several oral pathogens. | the effects of nd:yag laser irradiations at different power settings on several oral pathogens were evaluated. a total of 252 dentin samples were divided into seven groups consisting of 36 dentin specimens each. in each group, 9 of the 36 specimens were used as controls, thereby including a control in every group. the remaining 27 specimens were divided into three subgroups consisting of nine specimens according to different nd:yag laser settings (1.5, 1.8, and 2 w). each group was inoculated on ... | 2013 | 22767323 |
| nosocomial fungemia by candida auris: first four reported cases in continental europe. | candida auris is an emerging multidrug-resistant yeast that can cause invasive infections and is associated with high mortality. it is typically resistant to fluconazole and voriconazole and, some cases, also to echinocandins and amphotericin b. this species, phylogenetically related to candida haemulonii, is frequently misidentified by commercial identification techniques in clinical laboratories; therefore, the real prevalence of c. auris infections may be underestimated. | 2017 | 28131716 |
| an additional meyerozyma guilliermondii imh3 gene confers mycophenolic acid resistance in fungal ctg clade species. | the fungal ctg clade comprises a number of well-known yeasts that impact human health or with high biotechnological potential. to further extend the set of molecular tools dedicated to these microorganisms, the initial focus of this study was to develop a mycophenolic acid (mpa) resistance cassette. surprisingly, while we were carrying out preliminary susceptibility testing experiments in a set of yeast species, meyerozyma guilliermondii, although not being a mpa producer, was found to be primar ... | 2016 | 27620459 |
| structure-based designing of sordarin derivative as potential fungicide with pan-fungal activity. | fungal infections have become a significant problem for immunosuppressed patients. sordarin, a promising fungicidal agent, inhibits fungal protein synthesis by impairing elongation factor-2 (eef2) function. intriguingly, despite high sequence similarity among eef2s from different species, sordarin has been shown to inhibit translation specifically in certain fungi while unable to do so in some other fungal species (e.g. candida parapsilosis and candida lusitaniae). the sordarin binding site on e ... | 2016 | 27060894 |
| novel yeast isolated from broilers' feedstuff, gut and faeces as aflatoxin b1 adsorbents. | to isolate and characterize native yeast strains from broilers' environment as feedstuff, faeces and gut, and to evaluate their binding capacity for aflatoxin b1 (afb1 ). | 2016 | 27638385 |
| determination of yeast diversity in ogi, mawè, gowé and tchoukoutou by using culture-dependent and -independent methods. | the maize based ogi and mawè and the sorghum based gowé and tchoukoutou are traditional, spontaneously fermented products widely consumed by the population of benin (west africa). yeast occurrence in the products, as sold on local markets at different locations, was studied using a combination of culture-dependent and independent methods. number of yeasts is varied from 3.75 log10 colony forming units (cfu)/g for ogi to 5.60 log10 cfu/g for tchoukoutou. isolated yeasts (236) were identified base ... | 2013 | 23727651 |
| yeasts from colombian kumis as source of peptides with angiotensin i converting enzyme (ace) inhibitory activity in milk. | this study investigated the possibility of using yeast strains in fermented milks to obtain products with high angiotensin i-converting enzyme (ace) inhibitory activity and low bitter taste. ninety-three yeast strains isolated from colombian kumis in different geographic regions were molecularly identified, and their milk fermentation performances were determined. molecular identification evidenced that galactomyces geotrichum, pichia kudriavzevii, clavispora lusitaniae and candida tropicalis, w ... | 2012 | 22938834 |
| a stable phylogeny of the large-spored metschnikowia clade. | draft genomes of 55 strains representing all known large-spored metschnikowia species were used to construct a robust phylogeny of these yeasts found in association with flower-visiting insects. the genomes were annotated with reference to clavispora lusitaniae. from 3016 orthologues identified, 1061 were present in all strains with enough overlap to generate alignments of 500 bp or more. we constructed trees for all those alignments and evaluated their accuracy from their ability to resolve eac ... | 2016 | 27019191 |
| evaluation and characterization of new α-l-rhamnosidase-producing yeast strains. | a total of thirty yeast strains were isolated from a whey beverage and screened for α-l-rhamnosidase enzyme production. of these, only four isolates were capable of producing the α-l-rhamnosidase enzyme by hydrolyzing naringin. scanning electron microscopy images showed that the morphology of the yeast isolate (isolate no. 84) producing the greatest enzyme, changed from oval to filamentous in the presence of naringin. on the basis of morphological and molecular characterization (its sequencing), ... | 2015 | 26582283 |
| genetic diversity of medically important and emerging candida species causing invasive infection. | genetic variation in the ribosomal dna (rdna) internal transcribed spacer (its) region has been studied among fungi. however, the numbers of its sequence polymorphisms in the various candida species and their associations with sources of invasive fungal infections remain poorly investigated. here, we characterized the intraspecific and interspecific its diversity of candida spp. strains collected from patients with bloodstream or oroesophageal candidiasis. | 2015 | 25887032 |
| genetic diversity of clavispora lusitaniae isolated from agave fourcroydes lem, as revealed by dna fingerprinting. | this study characterized clavispora lusitaniae strains isolated from different stages of the processing and early fermentation of a henequen (agave fourcroydes) spirit produced in yucatan, mexico using a molecular technique. sixteen strains identified based on morphological features, obtained from different substrates, were typed molecularly. nine different versions of the divergent d1/d2 domain of the large-subunit ribosomal dna sequence were identified among the c. lusitaniae strains. the grea ... | 2015 | 25557477 |
| an ene reductase from clavispora lusitaniae for asymmetric reduction of activated alkenes. | a putative ene reductase gene from clavispora lusitaniae was heterologously overexpressed in escherichia coli, and the encoded protein (cler) was purified and characterized for its biocatalytic properties. this nadph-dependent flavoprotein was identified with reduction activities toward a diverse range of activated alkenes including conjugated enones, enals, maleimide derivative and α,β-unsaturated carboxylic esters. the purified cler exhibited a relatively high activity of 7.3 u mg(prot)⁻¹ for ... | 2014 | 24564901 |
| yeasts from autochthonal cheese starters: technological and functional properties. | the aim of this work was to identify 20 yeasts isolated from autochthonal cheese starters and evaluate their technological and functional properties. | 2013 | 23600736 |
| the dominant microbial community associated with fermentation of obushera (sorghum and millet beverages) determined by culture-dependent and culture-independent methods. | obushera includes four fermented cereal beverages from uganda namely: obutoko, enturire, ekitiribita and obuteire, whose microbial diversity has not hitherto been fully investigated. knowledge of the microbial diversity and dynamics in these products is crucial for understanding their safety and development of appropriate starter cultures for controlled industrial processing. culture-dependent and culture-independent techniques including denaturating gradient gel electrophoresis (dgge) and mixed ... | 2012 | 23141639 |
| the combined rapid detection and species-level identification of yeasts in simulated blood culture using a colorimetric sensor array. | a colorimetric sensor array (csa) has been demonstrated to rapidly detect and identify bacteria growing in blood cultures by obtaining a species-specific "fingerprint" of the volatile organic compounds (vocs) produced during growth. this capability has been demonstrated in prokaryotes, but has not been reported for eukaryotic cells growing in culture. the purpose of this study was to explore if a disposable csa could differentially identify 7 species of pathogenic yeasts growing in blood culture ... | 2017 | 28296967 |
| yeasts and hygienic-sanitary microbial indicators in water buffalo mozzarella produced and commercialized in minas gerais, brazil. | the aim of this work was to study the yeast populations and the main hygienic-sanitary microbial indicators in water buffalo mozzarella produced and commercialized in minas gerais, brazil. forty-two water buffalo mozzarella samples were purchased from retail outlets in belo horizonte. in addition, five samples of consecutive starter cultures, curd before acidification, acidified curd and mozzarella were collected at an industry in the city of oliveira. only three of the five water samples analyz ... | 2013 | 24516436 |
| survey of mycotic mastitis in dairy cows from heilongjiang province, china. | a survey of the prevalence rate, pathogenic subspecies, and risk factors of mycotic mastitis in dairy cows from heilongjiang province, china, was conducted. milk samples from 412 cows with chronic mastitis were collected and cultured on 8 % sheep blood agar, macconkey agar, and sabouraud agar with chloramphenicol. counting of the morphologically distinct colonies was performed, as well as the isolation and identification of organisms through phenotypical and physiological criteria. four hundred ... | 2013 | 23813119 |
| activity of four antimicrobial cocktails for tissue allograft decontamination against bacteria and candida spp. of known susceptibility at different temperatures. | several antimicrobial cocktail solutions of differing composition and concentrations are widely used to decontaminate viable banked tissue allografts at different temperatures and times of exposure. we compared the efficiency of four cocktails comprising nine antimicrobials to kill suspensions of a panel of 27 strains of 13 bacterial species, and 3 candida spp. at 4, 22 and 37 °c for 24 h. all but one bacterial strains were susceptible to one or more of the agents tested individually at concentr ... | 2014 | 23765096 |
| long-term follow up of complete bladder exstrophy repair. a case report. | bladder exstrophy is a congenital anomaly which is not always successfully managed by surgery. major goals of surgical intervention in such cases are preservation of normal renal function, development of adequate bladder function and urinary continence and avoidance of future urinary tract infections. we present 5-year data on a patient who underwent complete repair of the bladder exstrophy. | 2014 | 24812925 |
| evaluation of galactose adapted yeasts for bioethanol fermentation from kappaphycus alvarezii hydrolyzates. | bioethanol was produced from kappaphycus alvarezii seaweed biomass using separate hydrolysis and fermentation (shf). pretreatment was evaluated for 60 min at 121°c using 12% (w/v) biomass slurry with 364 mm h2so4. enzymatic saccharification was then carried out at 45°c for 48 h using celluclast 1.5 l. ethanol fermentation with 12% (w/v) k. alvarezii hydrolyzate was performed using the yeasts saccharomyces cerevisiae kctc1126, kluyveromyces marxianus kctc7150, and candida lusitaniae atcc42720 wit ... | 2016 | 27056472 |
| identification of some yeasts by fatty acid profiles. | 178 yeasts isolated from various foods were identified using id32 c strip; saccharomyces cerevisiae (47), rhodotorula mucilaginosa (19), candida kefyr (33), candida krusei (37), candida lusitaniae (28), cndida lipolytica (14). the yeasts were harvested, saponified, derivatized, and extracted, and fatty acid analysis was performed by gas chromatography mass spectrometry. the yeast lipids contained decanoic acid (c10:0), luaric acid (c12:0), myristoleic acid (c14:1), myristic acid (c14:0), pentade ... | 2014 | 25804068 |
| pulsed electromagnetic field assisted in vitro electroporation: a pilot study. | electroporation is a phenomenon occurring due to exposure of cells to pulsed electric fields (pef) which leads to increase of membrane permeability. electroporation is used in medicine, biotechnology, and food processing. recently, as an alternative to electroporation by pef, pulsed electromagnetic fields (pemf) application causing similar biological effects was suggested. since induced electric field in pemf however is 2-3 magnitudes lower than in pef electroporation, the membrane permeabilizat ... | 2016 | 27634482 |
| growth inhibition and membrane permeabilization of candida lusitaniae using varied pulse shape electroporation. | candida lusitaniae is an opportunistic yeast pathogen, which can readily develop resistance to antifungal compounds and result in a complex long-term treatment. the efficient treatment is difficult since structure and metabolic properties of the fungal cells are similar to those of eukaryotic host. one of the potential methods to improve the inhibition rate or the cell permeability to inhibitors is the application of electroporation. in this work we investigated the dynamics of the growth inhibi ... | 2015 | 26697485 |
| functional dissection of hamp domains in nik1 ortholog from pathogenic yeast candida lusitaniae. | nik1 orthologs or group iii hybrid histidine kinases (hhk) are ubiquitous signaling molecules in fungal pathogens. besides osmosensing, they are also involved in hyphal morphogenesis, virulence, and conidiation. they are important molecular targets for antifungal agents. nik1 orthologs contain a varying number of hamp domain repeats (poly-hamp) in the n-terminal region. poly-hamp plays a crucial role in their function. so far, the role of hamp domains in their function has been studied only in a ... | 2016 | 26657037 |
| regional centromeres in the yeast candida lusitaniae lack pericentromeric heterochromatin. | point centromeres are specified by a short consensus sequence that seeds kinetochore formation, whereas regional centromeres lack a conserved sequence and instead are epigenetically inherited. regional centromeres are generally flanked by heterochromatin that ensures high levels of cohesin and promotes faithful chromosome segregation. however, it is not known whether regional centromeres require pericentromeric heterochromatin. in the yeast candida lusitaniae, we identified a distinct type of re ... | 2015 | 26371315 |
| [molecular identification of candida lusitaniae in lower respiratory tract infection]. | candida lusitaniae is a yeast that has emerged as a low frequency nosocomial pathogen in deep infections. although it usually shows in vitro susceptibility to all antifungal agents, in vivo resistance to amphotericin b has been observed in several clinical cases. therefore, its early identification in the course of therapy is important. we report the isolation of c. lusitaniae as an etiologic agent of a lower respiratory tract infection in a male patient. urine and sputum cultures were negative ... | 2015 | 25576413 |
| a fox2-dependent fatty acid ß-oxidation pathway coexists both in peroxisomes and mitochondria of the ascomycete yeast candida lusitaniae. | it is generally admitted that the ascomycete yeasts of the subphylum saccharomycotina possess a single fatty acid ß-oxidation pathway located exclusively in peroxisomes, and that they lost mitochondrial ß-oxidation early during evolution. in this work, we showed that mutants of the opportunistic pathogenic yeast candida lusitaniae which lack the multifunctional enzyme fox2p, a key enzyme of the ß-oxidation pathway, were still able to grow on fatty acids as the sole carbon source, suggesting that ... | 2014 | 25486052 |
| phenotypic switching of candida guilliermondii is associated with pseudohyphae formation and antifungal resistance. | switching between two cell types in fungi is called phenotypic switching, and it is commonly observed in pathogenic yeast. candida lusitaniae undergoes antifungal resistance-associated phenotypic switching and results in three colony colors: light brown, brown and dark brown. in this study, we included c. lusitaniae as control. this study had two objectives. first, we wanted to evaluate whether also a prevalent human pathogen c. guilliermondii can undergo phenotypic switching. second, our aim wa ... | 2015 | 25481846 |
| deletion of the uracil permease gene confers cross-resistance to 5-fluorouracil and azoles in candida lusitaniae and highlights antagonistic interaction between fluorinated nucleotides and fluconazole. | we characterized two additional membrane transporters (fur4p and dal4p) of the nucleobase cation symporter 1 (ncs1) family involved in the uptake transport of pyrimidines and related molecules in the opportunistic pathogenic yeast candida lusitaniae. simple and multiple null mutants were constructed by gene deletion and genetic crosses. the function of each transporter was characterized by supplementation experiments, and the kinetic parameters of the uptake transport of uracil were measured usi ... | 2014 | 24867971 |
| convergent evolution of a fused sexual cycle promotes the haploid lifestyle. | sexual reproduction is restricted to eukaryotic species and involves the fusion of haploid gametes to form a diploid cell that subsequently undergoes meiosis to generate recombinant haploid forms. this process has been extensively studied in the unicellular yeast saccharomyces cerevisiae, which exhibits separate regulatory control over mating and meiosis. here we address the mechanism of sexual reproduction in the related hemiascomycete species candida lusitaniae. we demonstrate that, in contras ... | 2014 | 24390351 |
| antimicrobial activity of nisin, thymol, carvacrol and cymene against growth of candida lusitaniae. | yeasts are tolerant to acid ph values, are able to grow in anaerobic media and have minimum nutrition requirements. these capabilities enable them to survive and even grow in foods prepared from acid fruits or vegetables. among yeasts, candida is one of the genus most frequently isolated from fruit juices. bacteriocins and essential oils from spices and aromatic herbs are an alternative to preservatives and other technological treatments and have the advantage that their natural origins do not l ... | 2015 | 24293188 |
| host-pathogen interaction and signaling molecule secretion are modified in the dpp3 knockout mutant of candida lusitaniae. | candida lusitaniae is an emerging opportunistic yeast and an attractive model to discover new virulence factors in candida species by reverse genetics. our goal was to create a dpp3δ knockout mutant and to characterize the effects of this gene inactivation on yeast in vitro and in vivo interaction with the host. the secretion of two signaling molecules in candida species, phenethyl alcohol (pea) and tyrosol, but not of farnesol was surprisingly altered in the dpp3δ knockout mutant. no and reacti ... | 2014 | 24191303 |
| identification of a novel class b scavenger receptor homologue in portunus trituberculatus: molecular cloning and microbial ligand binding. | class b scavenger receptors (srbs), which are present in mammals and insects, have been implicated in a wide range of functions. herein, a novel srb homologue, ptsrb, was cloned from the swimming crab, portunus trituberculatus. ptsrb has 538 amino acid residues, and it consists of two transmembrane regions, a large extracellular loop, and two intracellular tails. a phylogenetic analysis showed that ptsrb distinctly clustered with marsupenaeus japonicas srb-1 and most drosophila srb homologues, i ... | 2016 | 27633673 |
| three novel toll genes (pttoll1-3) identified from a marine crab, portunus trituberculatus: different tissue expression and response to pathogens. | the toll signaling pathway is one of the most important regulators of the immune response in both vertebrates and invertebrates. herein, three novel toll (pttoll1-3) cdna sequences were cloned from the swimming crab, portunus trituberculatus. pttoll1 has 1003 amino acid residues and consists of an extracellular domain containing 15 leucine-rich repeats (lrrs) and a cytoplasmic toll/interleukin-1 receptor (tir) domain of 139 residues. pttoll2 encodes 1196 peptides, with an extracellular domain co ... | 2015 | 26238349 |
| molecular identification of uncommon clinical yeast species in iran. | by using advanced detection/identification methods, the list of emerging uncommon opportunistic yeast infections is rapidly expanding worldwide. our aim in the present study was sequence-based species delineation of previously unidentified yeasts obtained from a clinically yeast collection. | 2015 | 28680981 |
| comparative evaluation of six chromogenic media for presumptive yeast identification. | the present study was undertaken to evaluate the discrimination ability of six chromogenic media in presumptive yeast identification. | 2017 | 28663328 |
| prevalence and antifungal susceptibility of candida species in a tertiary care hospital in islamabad, pakistan. | to determine the prevalence and antifungal susceptibility pattern of candida species. | 2017 | 28770873 |
| evaluation of candidemia in epidemiology and risk factors among cancer patients in a cancer center of china: an 8-year case-control study. | candidemia is the worldwide life-threaten disease, especially in cancer patients. this study was aimed to identify and evaluate the risk factors of candidemia in cancer patients, which will prompt the improvement on current therapeutic strategies and prognosis. | 2017 | 28768479 |
| use of rna-protein complexes for genome editing in non-albicans candida species. | clustered regularly interspaced short palindromic repeat (crispr)-cas9 genome modification systems have greatly facilitated the genetic analysis of fungal pathogens. in crispr-cas9 genome editing methods designed for use in candida albicans, dnas that encode the necessary components are expressed in the target cells. unfortunately, expression constructs that work efficiently in c. albicans are not necessarily expressed well in other pathogenic species within the genus candida or the related genu ... | 2017 | 28657070 |
| molecular identification and antifungal susceptibility pattern of non-albicans candida species isolated from vulvovaginal candidiasis | vulvovaginal candidiasis (vvc) is an important problem due to candida spp. the aim of this study was molecular identification, phylogenetic analysis, and evaluation of antifungal susceptibility of non-albicans candida isolates from vvc. | 2017 | 28688376 |
| rapid and accurate molecular identification of the emerging multidrug-resistant pathogen candida auris. | candida auris is an emerging multidrug-resistant fungal pathogen causing nosocomial and invasive infections associated with high mortality. c. auris is commonly misidentified as several different yeast species by commercially available phenotypic identification platforms. thus, there is an urgent need for a reliable diagnostic method. in this paper, we present fast, robust, easy-to-perform and interpret pcr and real-time pcr assays to identify c. auris and related species: candida duobushaemulon ... | 2017 | 28539346 |
| fungal diversity of "tomme d'orchies" cheese during the ripening process as revealed by a metagenomic study. | tomme d'orchies is an artisanal pressed and uncooked cheese produced and marketed in the north of france. this study aimed at showing the fungal microbiota evolution of this cheese using a metagenetic based illumina technology targeting the its2 domain of 5.8s fungal rdnas. to this end, samples were taken from the rind and the core of different cheeses, after 0, 1, 3, 7, 14 and 21days of ripening. the data underpinned the prevalence of yarrowia lipolytica and galactomyces geotrichum for both mic ... | 2017 | 28806689 |
| genome sequence of the yeast clavispora lusitaniae type strain cbs 6936. | clavispora lusitaniae, an environmental saprophytic yeast belonging to the ctg clade of candida, can behave occasionally as an opportunistic pathogen in humans. we report here the genome sequence of the type strain cbs 6936. comparison with sequences of strain atcc 42720 indicates conservation of chromosomal structure but significant nucleotide divergence. | 2017 | 28774979 |
| development of a crispr-cas9 system for efficient genome editing of candida lusitaniae. | candida lusitaniae is a member of the candida clade that includes a diverse group of fungal species relevant to both human health and biotechnology. this species exhibits a full sexual cycle to undergo interconversion between haploid and diploid forms. c. lusitaniae is also an emerging opportunistic pathogen that can cause serious bloodstream infections in the clinic and yet has often proven to be refractory to facile genetic manipulations. in this work, we develop a clustered regularly interspa ... | 2017 | 28657072 |
| [primary immunodeficiencies in seriously ill children: report of 3 clinical cases]. | primary immunodeficiency diseases (pid) are congenital disorders secondary to an impaired immune response. infections, autoimmune disorders, atopy, and lymphoproliferative syndromes are commonly associated with this disorder. | 2017 | 28288231 |
| decreased killing activity of micafungin against candida guilliermondii, candida lusitaniae, and candida kefyr in the presence of human serum. | currently, echinocandins are first-line drugs for treatment of invasive candidiasis. however, data on how serum influences killing activity of echinocandins against uncommon candida species are limited. therefore, the killing activity of micafungin in rpmi-1640 and in 50% serum was compared against candida guilliermondii, candida lusitaniae, and candida kefyr. minimum inhibitory concentration (mic) ranges in rpmi-1640 were 0.5-1, 0.12-0.25, and 0.06-0.12 mg/l, respectively. in 50% serum, mics in ... | 2017 | 28072553 |
| [primary immunodeficiencies in seriously ill children: report of 3 clinical cases]. | primary immunodeficiency diseases (pid) are congenital disorders secondary to an impaired immune response. infections, autoimmune disorders, atopy, and lymphoproliferative syndromes are commonly associated with this disorder. | 2016 | 27692490 |
| voriconazole and posaconazole therapy for experimental candida lusitaniae infection. | the in vitro activity of posaconazole (psc) and voriconazole (vrc) was tested by using time-kill studies against 3 strains of candida lusitaniae. both drugs showed fungistatic activity against all strains. the efficacy of those compounds was evaluated by reducing kidney fungal burden and by determining (1→3)-β-d-glucan serum levels in a murine model of invasive infection of c. lusitaniae. the therapies tested were vrc at 10, 25, or 40 mg/kg/day and psc at 5, 12.5, or 20 mg/kg/twice a day. all th ... | 2016 | 26456387 |
| acquired multidrug antifungal resistance in candida lusitaniae during therapy. | candida lusitaniae is usually susceptible to echinocandins. beta-1,3-glucan synthase encoded by fks genes is the target of echinocandins. a few missense mutations in the c. lusitaniae fks1 hot spot 1 (hs1) have been reported. we report here the rapid emergence of antifungal resistance in c. lusitaniae isolated during therapy with amphotericin b (amb), caspofungin (cas), and azoles for treatment of persistent candidemia in an immunocompromised child with severe enterocolitis and visceral adenovir ... | 2015 | 26438490 |
| candida lusitaniae mics to the echinocandins are elevated but fks-mediated resistance is rare. | mic values were generated for caspofungin, micafungin, and anidulafungin against 106 isolates of c. lusitaniae, and these values were compared to established epidemiologic cutoff values. the majority of isolates were wild type both by mic value as well as by fks1 hotspot sequencing. although c. lusitaniae isolates have mic values to the echinocandins that are elevated compared to other common species, with regard to known mechanisms of resistance to the echinocandins, isolates with mic values at ... | 2016 | 26429293 |
| [investigation of the changes in candida epidemiology]. | the incidence of fungal infections has increased in recent years. antifungal resistance is a major problem with increasing frequency due to the widespread use of antifungal agents in infections. identification of the candida species and susceptibility patterns with the appropriate tests for resistance and selection of the empirical agents used for treatment are important. the aim of the study was to evaluate the changes of the epidemiology of candida species and minimum inhibitory concentrations ... | 2015 | 26313283 |
| acute limb ischemia due to candida lusitaniae aortic valve endocarditis. | we present a case of a 35-year-old male with history of intravenous heroin use, who presented with acute limb ischemia and was found to have candida lusitaniae endocarditis. after an urgent intervention to salvage the right lower extremity, successful outcome of his infection was achieved by a combined surgical and antifungal treatment. | 2014 | 24751340 |
| in vitro pharmacodynamics and in vivo efficacy of fluconazole, amphotericin b and caspofungin in a murine infection by candida lusitaniae. | the in vitro activities of fluconazole (flc), amphotericin b (amb) and caspofungin (csp) were evaluated against three isolates of candida lusitaniae using time-kill curves. amb showed in vitro fungicidal activity, whilst flc and csp exerted mainly strain-dependent fungistatic activity. the in vivo efficacies of the three drugs were evaluated in a murine model of disseminated infection. the doses administered were flc 50 mg/kg/day, amb 0.8 mg/kg/day and csp 5 mg/kg/day. all three drugs were able ... | 2014 | 24360922 |
| calcineurin is required for pseudohyphal growth, virulence, and drug resistance in candida lusitaniae. | candida lusitaniae is an emerging fungal pathogen that infects immunocompromised patients including hiv/aids, cancer, and neonatal pediatric patients. though less prevalent than other candida species, c. lusitaniae is unique in its ability to develop resistance to amphotericin b. we investigated the role of the calcium-activated protein phosphatase calcineurin in several virulence attributes of c. lusitaniae including pseudohyphal growth, serum survival, and growth at 37°c. we found that calcine ... | 2012 | 22952924 |