| [isolation of the recombinant proteins ospc and the fragment flaa (f-flaa) from the western-siberian borrelia garinii nt29 isolates and the study of their immunochemical properties]. | the structural proteins ospc and flaa of the lyme disease (ld) agent are known to be the basic antigens, which induce the humoral immune response at the initial stage of the disease. the goal of this work was to obtain the recombinant ospc and a fragment of the flaa protein (f-flaa) from the western siberian borrelia garinii nt29 isolates and to assess the possibility of their use for the ld diagnosis. encoding regions of the ospc and f-flab genes were amplified using pcr inserted in the preb ex ... | 2008 | 18368777 |
| detection of borrelia burgdorferi sensu lato dna by pcr in serum of patients with clinical symptoms of lyme borreliosis. | lyme borreliosis is a disease caused by spirochaetes belonging to the genospecies complex borrelia burgdorferi sensu lato (s.l.) transmitted by ixodes ticks. at present, serology remains the main diagnostic tool for laboratory diagnosis of lyme borreliosis. recently, the pcr technique has been applied for diagnosis of b. burgdorferi s.l., but, until now, a reliable, easy-to-perform and sensitive method has not been described. here we present a new pcr-based method for the detection of both b. bu ... | 2008 | 18384365 |
| prevalence and diversity of lyme borreliosis bacteria in marine birds. | a potential role of seabirds in spreading lyme disease (lb) spirochetes over large spatial scales was suggested more than 10 years ago when borrelia garinii was observed in marine birds of both hemispheres. since then, there have been few studies examining the diversity of borrelia spp. circulating in seabirds, or the potential interaction between terrestrial and marine disease cycles. to explore these aspects, we tested 402 ixodes uriae ticks collected from five colonial seabird species by ampl ... | 2008 | 18394972 |
| borrelia-associated crystalline keratopathy with intracorneal detection of borrelia garinii by electron microscopy and polymerase chain reaction. | first report of a patient with borrelia-associated crystalline keratopathy with intracorneal evidence of borrelia garinii by polymerase chain reaction (pcr) and electron microscopy (em). | 2008 | 18434857 |
| borrelia bissettii isolates induce pathology in a murine model of disease. | the spirochete borrelia burgdorferi is a tick-borne pathogen that causes lyme disease. although b. burgdorferi sensu lato is a diverse group of bacteria, only three genospecies, b. burgdorferi sensu stricto, borrelia afzelii, and borrelia garinii, are known to be pathogenic and commonly recognized to cause human disease. to assess the potential of another common genospecies, borrelia bissettii, to induce disease, a mouse model was employed. two colorado isolates of b. bissettii (co-bb) induced l ... | 2008 | 18454594 |
| molecular and serological evidence of borrelia burgdorferi sensu lato in wild rodents in the czech republic. | the aim of the present study was to determine the frequency and spatial distribution of the borrelia species in wild rodents in the czech republic. in total, 293 muscle tissue samples and 106 sera from 293 wild rodents captured in north bohemia and north-east and south moravia were examined for the presence of borrelia spp. and antibodies. muscle samples were investigated with real-time polymerase chain reaction (pcr) with a reca primer set, with dna quantification and melting curve analysis, an ... | 2008 | 18454596 |
| improved laboratory diagnostics of lyme neuroborreliosis in children by detection of antibodies to new antigens in cerebrospinal fluid. | laboratory diagnostics in lyme neuroborreliosis need improvement. we hereby investigate 4 new recombinant or peptide borrelia antigens in cerebrospinal fluid in children with neuroborreliosis to evaluate their performance as diagnostic antigens. | 2008 | 18536620 |
| comparison of pcr methods and culture for the detection of borrelia spp. in patients with erythema migrans. | the sensitivities of two pcr assays and culture were compared for the detection of borrelia spp. in skin specimens of 150 patients with typical erythema migrans. in addition, the accuracy of the methods for the identification of borrelia spp. was compared by analysing culture isolates and material obtained directly from skin biopsy specimens. borrelia burgdorferi sensu lato was isolated from 73 (49%) of 150 skin biopsy specimens. using a nested pcr targeting the rrf-rrl region and a pcr targetin ... | 2008 | 18558937 |
| characterization of borrelia burgdorferi sensu lato isolates by pulsed-field gel electrophoresis after mlui restriction of genomic dna. | at least three borrelia species (borrelia afzelii, borrelia garinii and borrelia burgdorferi sensu stricto) cause disease in humans, but borrelia spielmanii, borrelia valaisiana, borrelia lusitaniae and borrelia bissettii have also been reported to be rare or potential causes of human disease in europe. pulsed-field gel electrophoresis after mlui restriction of the genomic dna (mlui large restriction fragment patterns, lrfps) represents one of several approaches that have been used to assess bor ... | 2008 | 18586084 |
| presence of pathogenic borrelia burgdorferi sensu lato in ticks and rodents in zhejiang, south-east china. | a molecular epidemiological survey was conducted to investigate the presence of pathogenic borrelia burgdorferi sensu lato (s.l.) species in the forest areas of zhejiang province, south-east china. a total of 182 ticks of 6 species and 200 rodents of 8 species were collected and individually examined for the presence of b. burgdorferi s.l. dna by nested pcr targeting the 5s-23s rrna intergenic spacer. forty-one ticks of four species, haemaphysalis concinna, haemaphysalis longicornis, rhipicephal ... | 2008 | 18628499 |
| a european pathogenic microorganism proteome database: construction and maintenance. | a relational database structure based on ms-access and mysql to store and manage proteomics data was established. this system may be used to publish two-dimensional electrophoretic proteomics data, and also may be accessed by external users who want to compare their own data with those in the databases. the maintenance of the database is managed centrally. the producers of proteomics data do not need to construct a database themselves. users can introduce mass spectra into the database, which al ... | 2002 | 18628887 |
| validation of cultivation and pcr methods for diagnosis of lyme neuroborreliosis. | borrelial infection may manifest with a wide range of clinical signs, and in many cases, microbiological findings are essential for a proper diagnosis. this study included 48 patients with a working clinical diagnosis of lyme neuroborreliosis, 45 patients with a working clinical diagnosis of suspected lyme neuroborreliosis, and a control group comprising 42 patients with tick-borne encephalitis and 21 neurosurgical patients. the aim of the study was to analyze and compare findings of two pcr met ... | 2008 | 18716226 |
| prevalence of avian influenza viruses, borrelia garinii, mycobacterium avium, and mycobacterium avium subsp. paratuberculosis in waterfowl and terrestrial birds in slovakia, 2006. | the prevalence of borrelia, mycobacteria and avian influenza virus (aiv) infections, together with the distribution of different aiv subtypes, was studied in migratory waterfowl and terrestrial birds trapped in three localities in slovakia during 2006. samples obtained from waterfowl captured in the senianske ponds area of eastern slovakia showed the highest diversity of aiv isolates. a total of 13 different subtypes were detected in 19 samples from this location (h1n2, h2n2, h3n2, h6n6, h7n6, h ... | 2008 | 18798030 |
| deciphering the ligand-binding sites in the borrelia burgdorferi complement regulator-acquiring surface protein 2 required for interactions with the human immune regulators factor h and factor h-like protein 1. | borrelia burgdorferi, the etiologic agent of lyme disease, employs sophisticated means to evade killing by its mammalian hosts. one important immune escape mechanism is the inhibition of complement activation mediated by interactions of the host-derived immune regulators factor h (cfh) and factor h-like protein 1 (cfhl1) with borrelial complement regulator-acquiring surface proteins (bbcrasps). bbcrasp-2 is a distinctive cfh- and cfhl1-binding protein that is produced by serum-resistant b. burgd ... | 2008 | 18824548 |
| differential role of passerine birds in distribution of borrelia spirochetes, based on data from ticks collected from birds during the postbreeding migration period in central europe. | borrelia spirochetes in bird-feeding ticks were studied in the czech republic. during the postbreeding period (july to september 2005), 1,080 passerine birds infested by 2,240 ixodes ricinus subadult ticks were examined. borrelia garinii was detected in 22.2% of the ticks, borrelia valaisiana was detected in 12.8% of the ticks, borrelia afzelii was detected in 1.6% of the ticks, and borrelia burgdorferi sensu stricto was detected in 0.3% of the ticks. after analysis of infections in which the bl ... | 2009 | 19060160 |
| molecular detection of borrelia bissettii dna in serum samples from patients in the czech republic with suspected borreliosis. | until recently, three spirochete genospecies were considered to be the causative agents of lyme borreliosis (lb) in europe: borrelia burgdorferi sensu stricto, borrelia afzelii and borrelia garinii. however, the dna of borrelia valaisiana, borrelia lusitaniae, borrelia spielmanii and borrelia bissettii has already been detected in samples of human origin, or the spirochetes were isolated from the patients with symptoms of lb. molecular analysis of 12 selected serum samples collected in the regio ... | 2009 | 19187198 |
| arthritogenicity of borrelia burgdorferi and borrelia garinii: comparison of infection in mice. | arthritogenicity, as determined by joint swelling and synovial histology, was compared between or within two borrelia genospecies that cause lyme arthritis in humans. the spirochete burden in bladder tissue (a site of spirochete persistence) was documented by quantitative polymerase chain reaction, and immune responses were analyzed. in c3h/hej mice, three b. burgdorferi isolates and two of the three b. garinii isolates induced severe arthritis and swelling. previous designation as invasive or n ... | 2009 | 19190223 |
| antimicrobial activity of three tick defensins and four mammalian cathelicidin-derived synthetic peptides against lyme disease spirochetes and bacteria isolated from the midgut. | in this study, chemically synthesized tick defensins and cathelicidin-derived mammalian peptides were used to investigate the activity spectrum against borrelia garinii and symbiotic stenotrophomonas maltophila. synthetic tick defensins showed antimicrobial activity against staphylococcus aureus but not b. garinii and s. maltophila. mammalian peptides which have cationic property similar to tick defensins, showed antimicrobial activity similar to tick defensins. the antimicrobial peptides in tic ... | 2009 | 19229642 |
| detection of anaplasma phagocytophilum and borrelia burgdorferi sensu lato in dogs in the czech republic. | the aim of this study is to present molecular, serologic, and clinical findings for dogs that were naturally infected with anaplasma phagocytophilum or borrelia burgdorferi sensu lato (s. l.) in the czech republic. this data can provide information relevant to human infection. in total, blood samples from 296 dogs and 118 engorged ticks were examined. samples were tested for a. phagocytophilum using polymerase chain reaction (pcr) amplification, nested pcr, and direct sequencing of the 16s rdna, ... | 2009 | 19271998 |
| lyme neuroborreliosis: manifestations of a rapidly emerging zoonosis. | lyme disease has a worldwide distribution and is the most common vector-borne disease in the united states. incidence, clinical manifestations, and presentations vary by geography, season, and recreational habits. lyme neuroborreliosis (lnb) is neurologic involvement secondary to systemic infection by the spirochete borrelia burgdorferi in the united states and by borrelia garinii or borrelia afzelii species in europe. enhanced awareness of the clinical presentation of lyme disease allows inclus ... | 2009 | 19346313 |
| [evidence of borrelia burgdorferi spirochetes in patients with early disseminated lyme borreliosis]. | fifteen patients with clinically documented early disseminated lyme borreliosis were screened for the presence of spirochetes. in vitro trials of isolation of the pathogen were positive in 8 blood and 1 cerebrospinal fluid (csf) samples. the positivity was further confirmed by electron microscopy, western blot analysis and pcr. however, succesfull dna extraction after using borrelia specific primers proved the positivity in 9 blood and 1 csf samples (67 %). restriction fragment length polymorphi ... | 2009 | 19488960 |
| integration of a tick-borne encephalitis virus and borrelia burgdorferi sensu lato into mountain ecosystems, following a shift in the altitudinal limit of distribution of their vector, ixodes ricinus (krkonose mountains, czech republic). | the altitudinal shift in the limit of ixodes ricinus occurrence above the previously established altitude of 750 m above sea level has been monitored over the long-term (2002-2008) in the krkonose mts. (giant mts.), the highest in the czech republic, along two vertical transects in their eastern and central parts (600-1020 and 600-1270 m). ticks were collected by flagging three times annually, and examined individually by pcr or rt-pcr for the presence of borrelia burgdorferi sensu lato or tick- ... | 2010 | 19492952 |
| complex population structure of lyme borreliosis group spirochete borrelia garinii in subarctic eurasia. | borrelia garinii, a causative agent of lyme borreliosis in europe and asia, is naturally maintained in marine and terrestrial enzootic cycles, which primarily involve birds, including seabirds and migratory passerines. these bird groups associate with, correspondingly, ixodes uriae and ixodes ricinus ticks, of which the latter species may bite and transmit the infection to humans. studies of the overlap between these two natural cycles of b. garinii have been limited, in part due to the absence ... | 2009 | 19513109 |
| a new borrelia species defined by multilocus sequence analysis of housekeeping genes. | analysis of lyme borreliosis (lb) spirochetes, using a novel multilocus sequence analysis scheme, revealed that ospa serotype 4 strains (a rodent-associated ecotype) of borrelia garinii were sufficiently genetically distinct from bird-associated b. garinii strains to deserve species status. we suggest that ospa serotype 4 strains be raised to species status and named borrelia bavariensis sp. nov. the rooted phylogenetic trees provide novel insights into the evolutionary history of lb spirochetes ... | 2009 | 19542332 |
| risk indicators for the tick ixodes ricinus and borrelia burgdorferi sensu lato in sweden. | the distributional area of the tick ixodes ricinus (l.), the primary european vector to humans of lyme borreliosis spirochaetes (borrelia burgdorferi sensu lato) and tick-borne encephalitis virus, appears to be increasing in sweden. it is therefore important to determine which environmental factors are most useful to assess risk of human exposure to this tick and its associated pathogens. the geographical distribution of i. ricinus in sweden was analysed with respect to vegetation zones and clim ... | 2009 | 19712153 |
| borrelia burgdorferi stimulates macrophages to secrete higher levels of cytokines and chemokines than borrelia afzelii or borrelia garinii. | to delineate the inflammatory potential of the 3 pathogenic species of borrelia burgdorferi sensu lato, we stimulated monocyte-derived macrophages from healthy human donors with 10 isolates each of b. burgdorferi, borrelia afzelii, or borrelia garinii recovered from erythema migrans skin lesions of patients with lyme borreliosis from the united states or slovenia. b. burgdorferi isolates from the united states induced macrophages to secrete significantly higher levels of interleukin (il)-8, ccl3 ... | 2009 | 19909078 |
| a comparative analysis of molecular markers for the detection and identification of borrelia spirochaetes in ixodes ricinus. | borrelia burgdorferi sensu lato, carried by ixodes ticks, is one of the most significant human pathogens, causing lyme disease. as there is no standardized pcr method for detection and identification of spirochaete dna, we carried out a comparative analysis using a set of complementary primers for three regions in the genomic dna of these bacteria (genes fla and rrs and the non-coding rrs-rrla region). dna extracted from 579 ixodes ricinus ticks was subjected to nested pcr. dna of the examined s ... | 2010 | 20007765 |
| prevalence of borrelia burgdorferi in ixodes ricinus ticks in belgrade area. | lyme borreliosis is vector-borne zoonosis. the causative agent of lyme borreliosis is a spirochete of borrelia burgdorferi (bb) sensu lato complex, which is transmitted by ticks of the ixodes ricinus complex. the aim of our paper is to estimate the prevalence of i. ricinus ticks, the level of their infectivity by bb, and the prevalence of certain genospecies of bb sensu lato in ixodide ticks inhabiting belgrade. | 2010 | 20017716 |
| prevalence of five pathogenic agents in questing ixodes ricinus ticks from western france. | in europe, ixodes ricinus ticks are vectors of many emerging pathogens, including borrelia burgdorferi sensu lato (sl), anaplasma phagocytophilum, spotted fever group rickettsia sp., babesia sp., and very likely bartonella sp. in this study, we looked for the presence of dna of these microorganisms in 572 ticks from two forests in the west of france. dna extraction and polymerase chain reaction (pcr) amplification were performed on individual nymphal, male, and female i. ricinus ticks. amplifica ... | 2010 | 20020814 |
| binding of the complement inhibitor c4b-binding protein to lyme disease borreliae. | the lyme disease spirochetes, borrelia burgdorferi sensu stricto, borrelia afzelii and borrelia garinii, are tick-borne pathogens that can cause chronic disseminated infections. to survive in the human host borreliae need to evade the immune system. it is already well known that b. burgdorferi ss. and b. afzelii bind the complement (c) alternative pathway inhibitor factor h from serum using ospe and crasp-1/bba68 proteins to escape c attack. in the presence of natural antibodies and in chronic i ... | 2010 | 20022381 |
| identification and functional characterisation of complement regulator acquiring surface protein-1 of serum resistant borrelia garinii ospa serotype 4. | b. burgdorferi sensu lato (sl) is the etiological agent of lyme borreliosis in humans. spirochetes have adapted themselves to the human immune system in many distinct ways. one important immune escape mechanism for evading complement activation is the binding of complement regulators factor h (cfh) or factor h-like protein1 (fhl-1) to complement regulator-acquiring surface proteins (crasps). | 2010 | 20146822 |
| [enzyme immunoassay-based analysis of ospc recombinant proteins from borrelia garinii and borrelia afzelii isolated in west siberia]. | to study the ability of ospc recombinant proteins from borrelia garinii and borrelia afzelii isolated in west siberia to interact with serum antibodies from patients with tick-borne borreliosis (tbb). | 2010 | 20218339 |
| disordered lymphoid purine metabolism contributes to the pathogenesis of persistent borrelia garinii infection in mice. | extracellular atp and adenosine are important regulators of immune responses; however, contribution of purinergic signaling to host defense during persistent microbial infections remains obscure. lyme borreliosis is a common arthropod-borne infection caused by borrelia burgdorferi sensu lato. in this study, we investigated whether lymphoid purinergic signaling contributes to the mechanisms by which borreliae species evade the immune system and trigger joint inflammation. intracutaneous inoculati ... | 2010 | 20357256 |
| direct detection of borrelia burgdorferi spirochetes in patients with early disseminated lyme borreliosis. | the detection of spirochetes in 15 patients with clinically documented early disseminated lb has been analysed when using cultivation method of the plasma or the cerebrospinal fluid, electron microscopy, commercial western blot and detecting the dna of the pathogen in vitro cultures by pcr-rflp. spirochetes were isolated in eight blood and one cerebrospinal fluid culture samples. in seven cases (47%), previous serodiagnostic laboratory tests were negative. borrelial dna was detected by pcr in 67 ... | 2009 | 20377044 |
| tlr2 utilization of borrelia does not induce p38- and ifn-beta autocrine loop-dependent expression of cd38, resulting in poor migration and weak il-12 secretion of dendritic cells. | lyme borreliosis is a tick-borne bacterial infection that in many cases is limited to the skin. however, in some patients the bacterium evades the immune response and disseminates into various organs. dendritic cells (dcs) are among the first cells to meet invading pathogens in the skin. we have previously shown that cd38, an ectoenzyme involved in the migration of dcs and generally upregulated by microbial stimuli, is not upregulated in borrelia garinii-stimulated dcs. in this paper, we charact ... | 2010 | 20393136 |
| coexistence of borrelia spp. and babesia spp. in ixodes ricinus ticks in middle germany. | altogether, 430 nymphs and 570 adult ixodes ricinus ticks were collected in 2006 (n = 506) and 2007 (n = 494) from a forest area in middle germany (thuringia). single ticks were investigated by polymerase chain reaction and restriction fragment length polymorphism or sequencing for borrelia spp. (ospa gene) and babesia spp. (18s rrna gene). overall, 27.0% (270/1000) were infected with borrelia species. out of these, borrelia garinii was detected most frequently (133/270)-especially, ospa serotyp ... | 2010 | 20420533 |
| serological survey of five zoonoses, scrub typhus, japanese spotted fever, tularemia, lyme disease, and q fever, in feral raccoons (procyon lotor) in japan. | we investigated the seroprevalence of five tick- or mite-borne zoonoses, scrub typhus (orientia tsutsugamushi), japanese spotted fever (rickettsia japonica), tularemia (francisella tularensis), lyme disease (borrelia afzelii and borrelia garinii), and q fever (coxiella burnetii), in feral raccoons (procyon lotor) captured in hokkaido and kanagawa prefectures in japan. of the 559 raccoons captured in hokkaido, 8 (1.4%), 3 (0.5%), 1 (0.2%), and 1 (0.2%) carried antibodies against o. tsutsugamushi ... | 2011 | 20553108 |
| growth, cysts and kinetics of borrelia garinii (spirochaetales: spirochaetacea) in different culture media. | the aim of the present paper was to evaluate cyst formation and growth parameters of borrelia garinii in a range of media differing in formulation and cost. a qualitative assessment of morphology and motility of b. garinii was conducted. all media were prepared aseptically and used in test tubes or petri dishes. for each medium, the initial spirochete concentration was standardized to 10(3) spirochets/ml. the following culture media were suitable to grow b. garinii: barbour-stoenner-kelly, brain ... | 2010 | 20835623 |
| [analysis of borrelia burgdorferi genostrains among patients with lyme disease]. | lyme borreliosis is a zoonose which can be transmitted to the humans during ixodes tick bite to the skin. the disease is caused by bacteria--spirochetes of the borrelia species, classified as borrelia burgdorferi strain. in poland lyme borreliosis is caused by borrelia garinii, borrelia afzelii and borrelia burgdorferi sensu stricto. the presence of borrelia spielmani was recently reported. | 2009 | 21033412 |
| distribution of borrelia burgdorferi sensu lato in china. | we genotyped 102 borrelia burgdorferi sensu lato strains isolated from ticks, animals, and patients in 11 provinces in china by pcr-restriction fragment length polymorphism (pcr-rflp) amplification of 5s (rrf)-23s (rrl) rrna gene spacer amplicons and multilocus sequence analysis (mlsa). the results showed that borrelia garinii was the main genotype in china (65/102) and that it was distributed mainly in northern china. borrelia afzelii was the second most frequently found species (22/102), and i ... | 2010 | 21106783 |
| interpretation criteria for standardized western blot for the predominant species of borrelia burgdorferi sensu lato in china. | western blotting (wb; immunoblotting) is a widely used tool for the serodiagnosis of lyme borreliosis (lb), but so far, no generally accepted criteria for its performance and interpretation have been established in china. the present study was designed to determine the criteria for standardized western blot for the predominant species of borrelia burgdorferi sensu lato in china, in which wb was produced with strain pd₉₁ as the representative strain attributed to predominant genospecies borrelia ... | 2010 | 21112481 |
| prevalence and genetic heterogeneity of borrelia burgdorferi sensu lato in ixodes ticks in belgium. | borrelia burgdorferi sensu lato is a genetically diverse group of spirochetes that includes the agent of lyme borreliosis in which genospecies tend to be associated with specific clinical features. the aim of the study was to determine the prevalence and genetic diversity of borrelia burgdorferi sensu lato in 524 ticks collected in woods of a western province of belgium. presence of spirochetes in ticks was determined by phase contrast microscopy. the mean infection rate of ticks was 12.0%. vari ... | 2010 | 21128558 |
| genetic diversity of borrelia burgdorferi sensu lato isolates from northeastern china. | thirty-two strains of borrelia burgdorferi sensu lato were isolated from ixodes persulcatus ticks collected from northeastern china from may to june in 2004 and 2005. restriction fragment length polymorphism (rflp) analysis and sequence analysis of 5s-23s rrna intergenic spacer revealed that 29 (90.6%) belonged to borrelia garinii, demonstrating b, c, and a unique pattern. the remaining three isolates (9.4%) were borrelia afzelii with pattern d. the phylogenetic analysis based on 5s-23s rrna int ... | 2010 | 21142958 |
| synanthropic birds influence the distribution of borrelia species: analysis of ixodes ricinus ticks feeding on passerine birds. | ixodes ricinus ticks collected from 835 birds and from vegetation in the czech republic were analyzed. host-seeking ticks (n = 427) were infected predominantly by borrelia afzelii (25%). ticks (n = 1,012) from songbirds (passeriformes) were infected commonly by borrelia garinii (12.1%) and borrelia valaisiana (13.4%). juveniles of synanthropic birds, eurasian blackbirds (turdus merula) and song thrushes (turdus philomelos), were major reservoir hosts of b. garinii. | 2010 | 21148704 |
| [isolation and identification of borrelia burgdorferi sensu lato from ticks in six provinces in china.] | objective: to understand the carrying status of borrelia burgdorferi in ticks from the mountain areas from six representative provinces, including jilin, shanxi, gansu, qinghai, guizhou and hunan in china. methods: flagging and trapping methods were used to collect ticks in forest area and culture medium was used to isolate the pathogen. nested-pcr was used to detect the germ-carrying rate of ticks. results: more than 2200 ticks from six representative provinces were collected and 1000 ticks wer ... | 2010 | 21223661 |
| evaluation of a real-time pcr assay for the detection, genotyping, and quantification of borrelia burgdorferi sensu lato in ixodes ticks in a routine laboratory setting. | testing of saved ixodes ticks for the presence of borrelia burgdorferi after biting a patient may be helpful to assess the risk of bacterial transmission and to decide upon preventive antibiotic treatment. a commercially available real-time pcr assay for the identification and quantification of borrelia burdorferi sensu lato species (lightmix kit for the detection of borrelia spp., tib molbiol) was evaluated. | 2011 | 21391467 |
| multilocus sequence typing implicates rodents as the main reservoir host of human-pathogenic borrelia garinii in japan. | multilocus sequence typing of borrelia garinii isolates from humans and comparison with rodent and tick isolates were performed. fifty-nine isolates were divided into two phylogenetic groups, and an association was detected between clinical and rodent isolates, suggesting that, in japan, human-pathogenic b. garinii comes from rodents via ticks. | 2011 | 21411595 |
| the expanding lyme borrelia complex--clinical significance of genomic species? | ten years after the discovery of spirochaetes as agents of lyme disease in 1982 in the usa, three genomic species had diverged from the phenotypically heterogeneous strains of borrelia burgdorferi isolated in north america and europe: borrelia afzelii, b. burgdorferi sensu stricto (further b. burgdorferi), and borrelia garinii. whereas b. burgdorferi remained the only human pathogen in north america, all three species are aetiological agents of lyme borreliosis in europe. another seven genospeci ... | 2011 | 21414082 |
| loss of plasmids of borrelia burgdorferi sensu lato during prolonged in vitro cultivation. | in the present study we analyzed stability of plasmid content in 34 borrelia strains of three different species (13 borrelia afzelii, 10 borrelia garinii and 11 borrelia burgodorferi sensu stricto) using pulse field gel electrophoresis (pfge). during long-term in vitro cultivation consisting of 50 passages, plasmid loss was established in 46% of b. afzelii, 40% of b. garinii and 36% of b. burgdorferi sensu stricto strains. loss of plasmids occurred as early as between the 5th and 10th passage, a ... | 2011 | 21419795 |
| correlation of borrelia burgdorferi sensu lato prevalence in questing ixodes ricinus ticks with specific abiotic traits in the western palearctic. | this meta-analysis of reports examining ticks throughout the western palearctic region indicates a distinct geographic pattern for borrelia burgdorferi sensu lato prevalence in questing nymphal ixodes ricinus ticks. the greatest prevalence was reported between the 5°e and 25°e longitudes based on an analysis of 123 collection points with 37,940 nymphal tick specimens (87.43% of total nymphs; 56.35% of total ticks in the set of reports over the target area). climatic traits, such as temperature a ... | 2011 | 21498767 |
| peromyscus maniculatus, a possible reservoir host of borrelia garinii, at the gannet islands, newfoundland and labrador. | abstract thirty-five deer mice, peromyscus maniculatus, were trapped on gannet cluster 2 (gc-2), one of a group of islands numbered by convention, of the gannet island archipelago and examined for ectoparasites. one species each of acari (ixodes uriae) and siphonaptera (orchopeas leucopus) were recovered. samples of mice favored males to females (3.4:1). twenty-nine percent (10) of the mice were free of ectoparasites. males were more heavily parasitized than females when both parasites were cons ... | 2011 | 21506809 |
| the importance of ixodes arboricola in transmission of rickettsia spp., anaplasma phagocytophilum, and borrelia burgdorferi sensu lato in the czech republic, central europe. | abstract wild birds are known to be a reservoir of infectious disease agents and disseminatory hosts of ticks. the purpose of this work was to obtain information about the occurrence of rickettsial, anaplasmal, and borrelial infections in some ticks that parasitize wild birds in the czech republic. a total of 549 subadult ticks of three species ixodes arboricola (75.0%), ixodes ricinus (23.1%), and haemaphysalis concinna (1.8%) were collected from 20 species of birds (passeriformes). rickettsiae ... | 2011 | 21612531 |
| comparison of erythema migrans caused by borrelia burgdorferi and borrelia garinii. | abstract background: a comparison of patients with erythema migrans due to borrelia garinii versus borrelia burgdorferi has not been reported. patients and methods: one hundred nineteen patients from new york state with erythema migrans caused by b. burgdorferi were compared with 116 patients from slovenia with erythema migrans due to b. garinii infection. results: patients with b. garinii infection were older, more often reported a tick bite, and developed larger lesions (median largest diamete ... | 2011 | 21612533 |
| decorin binding by dbpa and b of borrelia garinii, borrelia afzelii, and borrelia burgdorferi sensu stricto. | background. decorin adherence is crucial in the pathogenesis of lyme borreliosis. decorin-binding proteins (dbp) a and b are the adhesins that mediate this interaction. dbpa and b of borrelia garinii, borrelia afzelii, and borrelia burgdorferi sensu stricto (ss) differ in their amino acid sequence, but little attention has been paid to the potential difference in their decorin binding. methods. we expressed recombinant dbpa and dbpb of b. garinii, b. afzelii, and b. burgdorferi ss and studied th ... | 2011 | 21628660 |
| acylated cholesteryl galactosides are ubiquitous glycolipid antigens among borrelia burgdorferi sensu lato. | lyme disease (ld) is the most common tick-borne disease in the northern hemisphere. it is caused by borrelia burgdorferi sensu lato, in particular b. burgdorferi sensu stricto, b. garinii and b. afzelii. however, other genospecies have been implicated as causative factors of ld as well. b. burgdorferi exhibits numerous immunogenic lipoproteins, but due to strong heterogeneity, the use of these proteins for serodiagnosis and vaccination is hampered. we and others have identified acylated choleste ... | 2011 | 21635569 |
| detection of borrelia burgdorferi sensu lato and anaplasma phagocytophilum in small mammals and ectoparasites in hungary. | abstract the aim of our study was to investigate the presence of borrelia burgdorferi sensu lato (s.l.) and anaplasma phagocytophilum in small mammals and ticks using polymerase chain reaction and to gain information about the prevalence and possible coexistence of these pathogens at a selected site in hungary. two hundred seventy-seven small mammals were trapped in south-eastern hungary during 2009. tissue samples and a total of 831 ectoparasites (ixodes ricinus, ixodes acuminatus, haemaphysali ... | 2011 | 21736488 |
| [Southern taiga combined natural foci of spirochetoses]. | Study of possibility of existence of combined natural foci of spirochetoses (ixodes tick borrelioses and leptospiroses) in typical taiga forests, and their etiologic and reservoir-host structure. | 2011 | 22145345 |
| genetic diversity of borrelia burgdorferi sensu lato isolates from northeastern china. | thirty-two strains of borrelia burgdorferi sensu lato were isolated from ixodes persulcatus ticks collected from northeastern china from may to june in 2004 and 2005. restriction fragment length polymorphism (rflp) analysis and sequence analysis of 5s-23s rrna intergenic spacer revealed that 29 (90.6%) belonged to borrelia garinii, demonstrating b, c, and a unique pattern. the remaining three isolates (9.4%) were borrelia afzelii with pattern d. the phylogenetic analysis based on 5s-23s rrna int ... | 2011 | 21870355 |
| whole-genome sequences of two borrelia afzelii and two borrelia garinii lyme disease agent isolates. | human lyme disease is commonly caused by several species of spirochetes in the borrelia genus. in eurasia these species are largely borrelia afzelii, b. garinii, b. burgdorferi, and b. bavariensis sp. nov. whole-genome sequencing is an excellent tool for investigating and understanding the influence of bacterial diversity on the pathogenesis and etiology of lyme disease. we report here the whole-genome sequences of four isolates from two of the borrelia species that cause human lyme disease, b. ... | 2011 | 22123755 |
| Identification and characterization of Borrelia antigens as potential vaccine candidates against Lyme borreliosis. | The three Borrelia species, Borrelia afzelii, Borrelia burgdorferi and Borrelia garinii are the main species causing the most common tick-borne zoonosis, Lyme borreliosis. By applying a genomic approach relying on human antibodies we have identified 122 antigenic Borrelia proteins associated with Lyme borreliosis, including already known and published protective antigens. The heterogeneity of the Borrelia species causing Lyme borreliosis makes the search for conserved antigens providing broad pr ... | 2011 | 22100635 |
| Long-term intrathecal infusion of outer surface protein C from Borrelia burgdorferi causes axonal damage. | Lyme neuroborreliosis (LNB) is the most frequent tick-borne infectious disease of the central nervous system. In acute LNB and the rare chronic state of infection, patients can experience cognitive deficits such as attention and memory disturbances. During LNB, single compounds of Borrelia burgdorferi sensu lato are released into the subarachnoid space.To investigate the pathogenesis of neurologic dysfunction in LNB, we determined that the outer surface protein C (OspC), a major virulence factor ... | 2011 | 21865883 |
| whole-genome sequences of borrelia bissettii, borrelia valaisiana, and borrelia spielmanii. | it has been known for decades that human lyme disease is caused by the three spirochete species borrelia burgdorferi, borrelia afzelii, and borrelia garinii. recently, borrelia valaisiana, borrelia spielmanii, and borrelia bissettii have been associated with lyme disease. we report the complete genome sequences of b. valaisiana vs116, b. spielmanii a14s, and b. bissettii dn127. | 2012 | 22207749 |
| The genospecies B. burgdorferi s.l., isolated from ticks and from neurological patients with suspected Lyme borreliosis. | Lyme borreliosis (LB) is the most disseminated tick-borne disease in the Northern hemisphere, and infestation with ticks is one of the essential factors influencing transmission of the disease to humans. This work intends to compare the occurrence of borrelia circulating in indigenous ticks and in patients suffering from neurological diseases. | 2011 | 21876490 |
| global ecology and epidemiology of borrelia garinii spirochetes. | lyme borreliosis (lb) is a tick-transmitted infectious disease caused by borrelia burgdorferi sensu lato (s. l.). in europe, three different borrelia species are the main causative agents of lb: b. burgdorferi sensu stricto (s.s.), borrelia afzelii, and borrelia garinii. the latter depends heavily on birds as its main reservoir hosts. in fact, birds can act both as biological carriers of borrelia and transporters of infected ticks. the seasonal migration of many bird species not only aid in the ... | 2011 | 22957111 |
| transmission dynamics of borrelia burgdorferi s.l. in a bird tick community. | we examined the borrelia burgdorferi sensu lato circulation in a tick community consisting of three species (ixodes ricinus, i. frontalis, i. arboricola) with contrasting ecologies, but sharing two european songbird hosts (parus major and cyanistes caeruleus). parus major had the highest infestation rates, primarily due to larger numbers of i. ricinus, and probably because of their greater low-level foraging. the prevalence of borrelia in feeding ticks did not significantly differ between the tw ... | 2012 | 23279105 |
| serological detection of lyme borreliosis agents in patients from korea, 2005-2009. | laboratory tests are now being used to identify seropositive cases in patients suspected of having a lyme borreliosis (lb) infection. from 2005 to 2009, we analyzed the serological and epidemiological characteristics of 53 lb positive cases in korea using immunoblot assay. | 2011 | 24159447 |
| cxcl13 may improve diagnosis in early neuroborreliosis with atypical laboratory findings. | current guidelines regarding lyme neuroborreliosis [lnb] require the presence of intrathecal borrelia burgdorferi-specific antibody production for the definite diagnosis of lnb. however, about 20% of early stage infections present without an elevated antibody index. moreover, intrathecal b. burgdorferi specific antibody synthesis may persist long after successful therapy of lnb. recently published data indicate that cxcl13 seems to be a promising diagnostic tool for early stage lnb. in addition, ... | 2012 | 23228054 |
| genome sequence of borrelia garinii strain nmjw1, isolated from china. | we announce the draft genome sequence of borrelia garinii strain nmjw1, isolated from ixodes persulcatus in northeastern china. the 902,789-bp linear chromosome (28.4% gc content) contains 813 open reading frames, 33 trnas, and 4 complete rrnas. | 2012 | 23144406 |
| the lymphocyte transformation test for borrelia detects active lyme borreliosis and verifies effective antibiotic treatment. | borrelia-specific antibodies are not detectable until several weeks after infection and even if they are present, they are no proof of an active infection. since the sensitivity of culture and pcr for the diagnosis or exclusion of borreliosis is too low, a method is required that detects an active borrelia infection as early as possible. for this purpose, a lymphocyte transformation test (ltt) using lysate antigens of borrelia burgdorferi sensu stricto, borrelia afzelii and borrelia garinii and ... | 2012 | 23091571 |
| role of birds in dispersal of etiologic agents of tick-borne zoonoses, spain, 2009. | we amplified gene sequences from anaplasma phagocytophilum, borrelia garinii, b. valaisiana, b. turdi, rickettsia monacensis, r. helvetica, r. sibirica sibirica, and rickettsia spp. (including candidatus rickettsia vini) in ticks removed from birds in spain. the findings support the role of passerine birds as possible dispersers of these tick-borne pathogens. | 2012 | 22709801 |
| dipa, a pore-forming protein in the outer membrane of lyme disease spirochetes exhibits specificity for the permeation of dicarboxylates. | lyme disease borreliae are highly dependent on the uptake of nutrients provided by their hosts. our study describes the identification of a 36 kda protein that functions as putative dicarboxylate-specific porin in the outer membrane of lyme disease borrelia. the protein was purified by hydroxyapatite chromatography from borrelia burgdorferi b31 and designated as dipa, for dicarboxylate-specific porin a. dipa was partially sequenced, and corresponding genes were identified in the genomes of b. bu ... | 2012 | 22590556 |
| cspa from borrelia burgdorferi inhibits the terminal complement pathway. | in order to survive and persist in an immunocompetent human host, borrelia burgdorferi controls the human immune attack and blocks the damaging effects of the activated complement system. these gram-negative spirochetes use cspa (crasp-1) and four additional immune evasion proteins to bind combinations of human plasma regulators, including factor h, factor h-like protein 1 (fhl-1), complement factor h-related protein 1 (cfhr1), cfhr2, cfhr5, and plasminogen. as many microbial immune evasion prot ... | 2013 | 23943762 |
| whole-genome sequence of borrelia garinii strain 935t isolated from ixodes persulcatus in south korea. | we report here the genome sequence of borrelia garinii strain 935t isolated from ixodes persulcatus in south korea. the 1,176,739 bp (g+c content, 27.73%) genome consists of 1,194 coding regions, 4 rrna genes, and 33 aminoacyl-trna synthetase genes. this is the first whole-genome report of a korean borrelia species isolate. | 2014 | 25540341 |
| pathogens in ticks collected from dogs in berlin/brandenburg, germany. | tick-borne diseases are a major health risk for humans and dogs. in addition to collection and analysis of questing ticks, analysis of host-associated ticks for the presence of pathogens is a valuable method to gain insight into transmission patterns of tick-borne diseases. | 2014 | 25441762 |
| first detection and molecular identification of borrelia garinii spirochete from ixodes ovatus tick ectoparasitized on stray cat in taiwan. | borrelia garinii spirochete was detected for the first time in ixodes ovatus tick ectoparasitized on stray cat in taiwan. the genetic identity of this detected spirochete was determined by analyzing the gene sequence amplified by genospecies-specific polymerase chain reaction assays based on the 5s-23s intergenic spacer amplicon (rrf-rrl) and outer surface protein a (ospa) genes of b. burgdorferi sensu lato. phylogenetic relationships were analyzed by comparing the sequences of rrf-rrl and ospa ... | 2014 | 25343260 |
| borrelia garinii and rickettsia monacensis in ixodes ricinus ticks, algeria. | | 2014 | 25272139 |
| early cytokine release in response to live borrelia burgdorferi sensu lato spirochetes is largely complement independent. | here we investigated the role of complement activation in phagocytosis and the release of cytokines and chemokines in response to two clinical isolates: borrelia afzelii k78, which is resistant to complement-mediated lysis, and borrelia garinii lu59, which is complement-sensitive. | 2014 | 25265036 |
| the heterogeneity, distribution, and environmental associations of borrelia burgdorferi sensu lato, the agent of lyme borreliosis, in scotland. | lyme borreliosis is an emerging infectious human disease caused by the borrelia burgdorferi sensu lato complex of bacteria with reported cases increasing in many areas of europe and north america. to understand the drivers of disease risk and the distribution of symptoms, which may improve mitigation and diagnostics, here we characterize the genetics, distribution, and environmental associations of b. burgdorferi s.l. genospecies across scotland. in scotland, reported lyme borreliosis cases have ... | 2014 | 25221774 |
| genome sequence of borrelia garinii strain sz, isolated in china. | we announce the genome sequence of borrelia garinii strain sz, isolated from dermacentor ticks collected in northeastern china. b. garinii strain sz carries numerous plasmids, both 10 circular and 9 linear plasmids. the 902,487-bp linear chromosome (28.2% gc content) contains 820 open reading frames, 33 trnas, and 4 complete rrnas. the plasmid cp32-10 contains one clustered regularly interspaced short palindromic repeat (crispr) with four repeats. | 2014 | 25035317 |
| tick-borne zoonotic pathogens in birds in guangxi, southwest china. | wildlife is an important natural reservoir of many tick-borne pathogens. these agents have an impact on the health of humans and other animals throughout the world. this study was conducted to determine whether and what species of tick-borne agents had infected wild birds collected from guangxi, in southwest china. | 2015 | 26666827 |
| in vitro evaluation of antibacterial activity of phytochemicals and micronutrients against borrelia burgdorferi and borrelia garinii. | little is known about the effects of phytochemicals against borrelia sp. causing lyme disease. current therapeutic approach to this disease is limited to antibiotics. this study examined the anti-borreliae efficacy of several plant-derived compounds and micronutrients. | 2015 | 26457476 |
| infections and mixed infections with the selected species of borrelia burgdorferi sensu lato complex in ixodes ricinus ticks collected in eastern poland: a significant increase in the course of 5 years. | in the years 2008-2009 and 2013-2014, 1620 and 1500 questing ixodes ricinus ticks, respectively, were examined on the territory of the lublin province (eastern poland). the presence of three pathogenic species causing lyme disease was investigated: borrelia burgdorferi sensu stricto, b. afzelii and b. garinii. the proportion of i. ricinus ticks infected with b. burgdorferi sensu lato showed a highly significant increase between 2008-2009 and 2013-2014, from 6.0 to 15.3%. a significant increase w ... | 2015 | 26590929 |
| blood-borne candidatus borrelia algerica in a patient with prolonged fever in oran, algeria. | to improve the knowledge base of borrelia in north africa, we tested 257 blood samples collected from febrile patients in oran, algeria, between january and december 2012 for borrelia species using flagellin gene polymerase chain reaction sequencing. a sequence indicative of a new borrelia sp. named candidatus borrelia algerica was detected in one blood sample. further multispacer sequence typing indicated this borrelia sp. had 97% similarity with borrelia crocidurae, borrelia duttonii, and borr ... | 2015 | 26416117 |
| serological survey of borrelia infection of dogs in sapporo, japan, where borrelia garinii infection was previously detected. | a serological survey of borrelia infection of dogs was performed in sapporo, japan, where borrelia garinii infection in dogs was detected in 2011. a total of 314 serum samples were collected from dogs that visited three animal hospitals in sapporo from 2012 to 2014. the two-step evaluation method, involving screening elisa followed by western blot analysis, was used to detect antibodies against borrelia species. a total of 34 samples were positive by elisa. among those 34 samples, 32 were positi ... | 2015 | 26522809 |
| an invasive mammal (the gray squirrel, sciurus carolinensis) commonly hosts diverse and atypical genotypes of the zoonotic pathogen borrelia burgdorferi sensu lato. | invasive vertebrate species can act as hosts for endemic pathogens and may alter pathogen community composition and dynamics. for the zoonotic pathogen borrelia burgdorferi sensu lato, the agent of lyme borreliosis, recent work shows invasive rodent species can be of high epidemiological importance and may support host-specific strains. this study examined the role of gray squirrels (sciurus carolinensis) (n = 679), an invasive species in the united kingdom, as b. burgdorferi sensu lato hosts. w ... | 2015 | 25888168 |
| borrelia burgdorferi sensu lato in humans in a rural area of paraná state, brazil. | this study describes the detection of borrelia garinii and borrelia burgdorferi sensu stricto (s.s.) in brazilian individuals using pcr and dna sequencing. our results suggest that these species are emerging pathogens in this country, and additional studies are necessary to determine the epidemiological characteristics of this disease in brazil. | 2015 | 26273276 |
| prevalence of borrelia burgdorferi sensu lato in ticks from eastern china. | to explore the tick distribution and prevalence of borrelia in zhejiang province, we performed a survey in nine sites. a total of 447 adult ticks of 11 species were captured and the dominant tick species were haemaphysalis longicornis and ixodes sinensis and the abundance of tick species in different areas varied significantly. overall, 4.70% of the ticks were polymerase chain reaction (pcr) positive for borrelia. the average pcr positive rates were 5.19% for h. longicornis, 3.45% for amblyomma ... | 2015 | 25548382 |
| [long-term dynamics of the epizootic process in natural foci of ixodid tick borreliosis in mountain taiga forests of the middle ural]. | during eleven spring-summer seasons (1992-2002), the dynamics of the epizootic process in natural foci of ixodid tick-borne borrelioses (itbb) were studied in taiga forests of the middle ural (perm province, russia). in these foci, borrelia garinii and b. afzelii circulate, and their main vector is the ixodes persulcatus tick. main parameters of the epizootic process were calculated for each season. in four seasons (1993, 1996, 1999 and 2002), the parameters characterizing the abundance of unfed ... | 2004 | 15174388 |
| gencolors: accelerated comparative analysis and annotation of prokaryotic genomes at various stages of completeness. | summary: gencolors is a new web-based software/database system aimed at an improved and accelerated annotation of prokaryotic genomes, considering information on related genomes and making extensive use of genome comparison. it offers a seamless integration of data from ongoing sequencing projects and annotated genomic sequences obtained from genbank. the genome comparison tools determine, for example, best-bidirectional hits, gene conservation, syntenies and gene core sets. swiss-prot/trembl hi ... | 2005 | 16076887 |
| hosts and pathogen detection for immature stages of ixodes ricinus (acari: ixodidae) in north-central spain. | to determine hosts of the immature stages of a southern population of ixodes ricinus, we trapped rodents and birds in an area of north-central spain in may-june and august-september of 1998 and 1999. the most frequently trapped rodents were apodemus sylvaticus (230 specimens) and clethrionomys glareolus (99), with a larval infestation prevalence of 49% and 81%, respectively (in spring) and 21% and 41% (in summer). c. glareolus was always more heavily parasitized by larvae (mean numbers 19.8 in s ... | 2005 | 16323055 |
| adhesion of borrelia garinii to neuronal cells is mediated by the interaction of ospa with proteoglycans. | to study pathogenic mechanisms of lyme meningoradiculitis, dorsal root ganglia (drg) cells and two neuronal cell lines (b50, sh-sy5y) were incubated with borrelia garinii, the borrelia species most frequently isolated from csf of lyme neuroborreliosis patients in europe. we demonstrated that (i) ospa-positive b. garinii adhere to neuronal cells, (ii) borrelia adhesion can be blocked by a monoclonal antibody against ospa, (iii) preincubation with proteoglycans interferes with the adhesion process ... | 2006 | 16603253 |
| migratory passerine birds as reservoirs of lyme borreliosis in europe. | to define the role of birds as reservoirs and disseminators of borrelia spirochetes, we characterized tick infestation and reservoir competence of migratory passerine birds in sweden. a total of 1,120 immature ixodes ricinus ticks were removed from 13,260 birds and assayed by quantitative polymerase chain reaction (pcr) for borrelia, followed by dna sequencing for species and genotype identification. distributions of ticks on birds were aggregated, presumably because of varying encounters with t ... | 2006 | 16836825 |
| a novel lymphocyte transformation test (ltt-melisa) for lyme borreliosis. | diagnosis of active lyme borreliosis (lb) remains a challenge in clinically ambiguous, serologically indeterminant, and polymerase chain reaction-negative patients. lymphocyte transformation tests (ltts) have been applied to detect specific cellular immune reactivity, but their clinical application has been severely hampered by the poorly defined borrelia antigens and nonstandardized ltt formats used. in this study, we describe the development and clinical relevance of a novel ltt using a valida ... | 2007 | 16876371 |
| clinical appearance of erythema migrans caused by borrelia afzelii and borrelia garinii--effect of the patient's sex. | the aim in this survey was to study the clinical characteristics of infections caused by borrelia genospecies in patients with erythema migrans where borrelial origin was confirmed by polymerase chain reaction. the aim was also to study factors influencing the clinical appearance of erythema migrans. | 2006 | 17009065 |
| detection of borrelia garinii, borrelia tanukii and borrelia sp. closely related to borrelia valaisiana in ixodes ticks removed from dogs and cats in japan. | ticks removed from 1136 dogs and 134 cats all over japan were examined for borrelia infection by pcr and sequencing. the 5s-23s rdna intergenic spacer of borrelia was detected from two ixodes persulcatus ticks from two dogs and two unidentified ixodes spp. from another two dogs in hokkaido, and two ixodes granulatus ticks from two cats in okinawa. additional 2 i. granulatus from the same cats also showed positive. sequence analysis of the pcr products revealed that the one from hokkaido was simi ... | 2007 | 17049744 |
| seronegative lyme neuroborreliosis in a patient on treatment for chronic lymphatic leukemia. | we report on a patient who developed seronegative lyme neuroborreliosis complicating chemotherapy for chronic lymphatic leukemia. after the fifth cycle of chemotherapy (fcr: fludarabine, cyclophosphamide, rituximab and prednisone) the 63-year-old patient developed night sweat, arthralgia in elbows, wrists, proximal interphalangeal joints (pips) and strong neuropathic pain in both legs, followed by paresthesia and hypesthesia in the feet, arms and face. laboratory analysis revealed an elevated c- ... | 2007 | 17401717 |
| transcriptional response of human dendritic cells to borrelia garinii--defective cd38 and ccr7 expression detected. | lyme borreliosis is a disease, which can affect several organs and cause a variety of symptoms. in some patients, the infection may become chronic, even after antibiotic therapy, and cause persisting damage. dendritic cells (dc) are involved in the initiation of innate and adaptive immune responses. to study interactions between borrelia garinii (bg), one of the causative agents of lyme borreliosis, and human dc, we used a cdna microarray to compare the bg-induced dc transcriptional response wit ... | 2007 | 17440035 |
| [eye involvement of borrelia aetiology]. | we present a case of eye involvement -- intermediate uveitis -- during tick-borne borreliosis in a 10-year-old boy. ophthalmologic examination revealed impaired vision, apparent thick floating whitish opacity in the vitreous humour of the left eye and fine fibres in the vitreous humour of the right eye. sonographic examination confirmed hyperechogenic opacity in the vitreous humour. an autoimmune process was suspected but not confirmed. serological examination showed igg antibodies against three ... | 2007 | 17703406 |
| antigen biochips verify and extend the scope of antibody detection in lyme borreliosis. | the antibody response of serum igm and igg of patients with neuroborreliosis and erythema migrans of lyme borreliosis (lb) was examined against a 41-kda flagellar antigen and an 8-mer synthetic ospc8 peptide (vaespkkp) derived from the c-terminus of outer surface protein c (ospc) from borrelia garinii. we developed a streptavidin-modified biochip-based immunodiagnosis and compared it with conventional methods such as enzyme-linked immunosorbent assay (elisa) and western blot (wb). the diagnostic ... | 2007 | 17888607 |
| [isolation of tick-borne borreliosis agent from blood of patients]. | during spring-autumn period of 2006 borrelia were isolated for the first time in russia from blood of 79 patients treated in perm city clinical hospital for infectious diseases no. 1 with diagnosis "tickborne borreliosis, manifestive form with migrating erythema, localized stage". ten primary isolates (12.7% of total seeded samples) were obtained by seeding plasma samples on the bsk medium. their subsequent identification by polymerase chain reaction-restriction fragments length polymorphism rev ... | 2009 | 19340967 |