| influence of temperature, salinity and e. coli tissue content on immune gene expression in mussel: results from a 2005-2008 survey. | several bivalves, including mussels, suffered from mortalities particularly in summer. to look for the possible effect of environmental parameters on immune capacities, mytilus galloprovincialis were collected monthly from august 2005 to july 2008 from the palavas laguna, french mediterranean coast. q-pcr was used to quantify the expression of three antimicrobial peptide genes (defensin, mytilin b and myticin b), in addition to lysozyme and hsp70. house keeping gene was 28s rrna. defensin, mytic ... | 2009 | 19409926 |
| distribution of synthetic organotins and total tin levels in mytilus galloprovincialis along the portuguese coast. | despite the huge amount of literature available on butyltins (buts), few studies addressed the environmental levels of phenyltins (phts), octyltins (octs) and total tin (sn(t)) in environmental samples. in 2006 a mussel watch survey was developed for the portuguese coast (total of 29 sampling sites) in order to describe the concentrations of buts , phts, octs and sn(t) in the whole tissues of mytilus galloprovincialis (lamarck, 1819). buts were detected in all analyzed samples accounting, in ave ... | 2009 | 19426996 |
| detecting micronuclei frequency in some aquatic organisms for monitoring pollution of izmir bay (western turkey). | micronuclei tests is a system of mutagenicity testing used for determining the pollution and chemicals causing changes in dna fragments such as micronuclei in the cytoplasm of interphase cells. damage caused on the dna by genotoxic pollutants is the first consequence occurring in the aquatic organisms. thus, it was attempted to determine whether pollution affected the erythrocytes and gills of fish gobius niger and haemolymph and gills of mussels mytilus galloprovincialis living in izmir bay at ... | 2010 | 19444638 |
| first evidence of fiberglass ingestion by a marine invertebrate (mytilus galloprovincialis l.) in a n.w. mediterranean estuary. | alfacs bay is a n.w. mediterranean estuary important for mussel (mytilus galloprovincialis) aquaculture. during studies at the site, fiberglass particles were detected. the presence of fiberglass occurred naturally in the water throughout the study period (november 2006 to july 2007). an investigation was undertaken into its role in the feeding behavior of the local mussels. fiberglass was present in all types of mussel samples. rejection, which we would have expected for the whole study period, ... | 2009 | 19476956 |
| biomarkers of damage and protection in mytilus galloprovincialis cross transplanted in ria formosa lagoon (portugal). | the study was carried out to check the water contamination in two sites of ria formosa lagoon by assessing the damage responses versus protection in the gills and digestive gland (dg) of mussels--mytilus galloprovincialis lmk. damage was assessed as dna integrity and lipid peroxidation (lpo) while protection was evaluated by measuring catalase (cat), glutathione peroxidase (gpx), glutathione reductase (gr), glutathione s-transferase (gst), total glutathione (gsht) and metallothioneins (mt). muss ... | 2009 | 19609672 |
| secretion of byssal threads in mytilus galloprovincialis: quantitative and qualitative values after spawning stress. | the effect of spawning events of the mussel mytilus galloprovincialis on both quantitative and qualitative values of byssus secretion and its associated attachment force was investigated. byssogenesis rates and absorption efficiency values were significantly reduced after spawning of individuals. however, the maintenance of individuals under sub-optimal conditions (lack of microalgae in the diet) for a week caused no effect on thread's number. surprisingly, the attachment force varied within a n ... | 2010 | 19618191 |
| effects of environmental concentrations of the antiepilectic drug carbamazepine on biomarkers and camp-mediated cell signaling in the mussel mytilus galloprovincialis. | pharmaceuticals represent a diverse collection of thousands of bioactive chemicals used in human and veterinary medicine. the increased consumption, together with the recent development of more sensitive analytical techniques, has identified these as emerging contaminants in the aquatic environment. according to many investigations pharmaceuticals do not cause acute toxic effects in organisms when released in the environment. however, many independent studies agree that chronic exposure and more ... | 2009 | 19632730 |
| complex toxin profile of mytilus galloprovincialis from the adriatic sea revealed by lc-ms. | this paper reports on the determination of toxin profile of mussels (mytilus galloprovincialis) collected in november-december 2003 along the emilia romagna coasts (italy) when a high concentration of alexandrium ostenfeldii cells was detected in seawater. detailed liquid chromatography-mass spectrometry (lc-ms) analyses were performed on the crude extracts in both selected ion monitoring (sim) and multiple reaction monitoring (mrm) modes. they revealed that m. galloprovincialis had accumulated ... | 2010 | 19660488 |
| coccomyxa sp. (chlorophyta: chlorococcales), a new pathogen in mussels (mytilus galloprovincialis) of vigo estuary (galicia, nw spain). | in this work, we describe the occurrence of irregular shaped green aggregations in the mantle, gill filaments, adductor muscle, visceral mass and haemolymph of wild mussels (mytilus galloprovincialis) collected from the vigo estuary (galicia, nw spain). microscopic examination of these masses revealed that they consist of intracellular green algae which are spherical to oval in shape, 5 microm in length and 3 microm in width, without flagella and with a smooth surface. the algal cells present a ... | 2009 | 19686756 |
| a multibiomarker approach in mytilus galloprovincialis to assess environmental quality. | a multibiomarker approach was carried out for the first time in the south portuguese coast using mytilus galloprovincialis, to assess environmental quality, establish if there are adverse biological responses associated to different sources of anthropogenic contamination and to determine spatial and seasonal trends. for this purpose the battery of biomarkers selected was: superoxide dismutase (sod), catalase (cat), glutathione peroxidases (gpx total and se dependent), cytochrome p450 component s ... | 2009 | 19724838 |
| parasites and symbionts from mytilus galloprovincialis (lamark, 1819) (bivalves: mytilidae) of the aveiro estuary portugal. | the primary aim of the present study was to conduct a 1-yr parasitological survey of a mytilus galloprovincialis mussel population from the aveiro estuary, portugal. in addition, we attempted to assess the host response to parasites by examining host histopathology, including an evaluation of hemocyte infiltration and parasite encapsulation. the parasites present (prevalence) included the following: 1 protozoan, nematopsis sp. (70%); 1 turbellarian, urastoma cyprinae, (39%); 2 trematodes, diphth ... | 2010 | 19785477 |
| effects on feeding rate and biomarker responses of marine mussels experimentally exposed to propranolol and acetaminophen. | environmental risk assessments of human pharmaceuticals and other 'emerging contaminants' should integrate both population-relevant endpoints and biomarkers of potential modes of action in a range of species. adult mytilus galloprovincialis were exposed to the beta-adrenergic receptor blocker propranolol or to the anti-inflammatory drug acetaminophen (paracetamol), both commonly used therapeutic drugs present in aquatic ecosystems. mussels were exposed under semi-static conditions for 10 days to ... | 2010 | 19838684 |
| cholinesterase and glutathione s-transferase activities of three mollusc species from the nw portuguese coast in relation to the 'prestige' oil spill. | in november 2002, the tanker 'prestige' released about 19,000 tonnes of a heavy fuel oil (no. 6) before sinking with about 58,000 tonnes of its cargo, 135 miles from cabo finisterra (spain). a considerable part of the released fuel oil reached the galician coast, causing a heavy black tide and an ecological disaster. although the black tide did not reach the nw coast of portugal, it is possible that some of the fuel oil or its components also arrived to this area directly through the sea water a ... | 2009 | 19889444 |
| integrated use of antioxidant enzymes in mussels, mytilus galloprovincialis, for monitoring pollution in highly productive coastal areas of galicia (nw spain). | in the present work, we investigated the potential use of several antioxidant enzymes in wild mussels (mytilus galloprovincialis) as biomarkers of marine pollution. the enzymatic activity levels of glutathione s-transferase (gst), glutathione peroxidase (gpx) and catalase (cat) were measured in gills and digestive gland. those enzymes participate in the cellular defense system that is involved in the adaptive response of organisms to chemical pollution. adult mussels were collected at five sampl ... | 2010 | 19954813 |
| integrated assessment of water quality of the costa da morte (galicia, nw spain) by means of mussel chemical, biochemical and physiological parameters. | the aim of this study was to assess environmental quality at some of the sites most severely affected by the prestige oil spill off 2 years after the spillage (april and november 2004). for this purpose analyses of polycyclic aromatic hydrocarbons (pah) and several biochemical (antioxidant enzymes catalase, glutathione reductase, glutathione peroxidase, superoxide dismutase and dt-diaphorase and lipid peroxidation) and physiological [scope for growth (sfg)] biomarkers were determined on wild mus ... | 2010 | 20033486 |
| expression of mytilus immune genes in response to experimental challenges varied according to the site of collection. | mussels live in diverse coastal environments experience various physical, chemical and biological conditions, which they counteract with functional adjustments and heritable adaptive changes. in order to investigate possible differences in immune system capabilities, we analyzed by qpcr the expression levels of 4 immune genes (defensin, mytilin b, myticin b, lysozyme) and hsp70 in the mediterranean mussel, mytilus galloprovincialis collected in 3 european farming areas {atlantic ocean-ría de vig ... | 2010 | 20045066 |
| effects of sodium dodecylbenzene sulfonate and sodium dodecyl sulfate on the mytilus galloprovincialis biomarker system. | the effects of in vivo exposure of mytilus galloprovincialis to two anionic surfactants (sdbs and sds) on the molecular biomarker system were studied. after continuous exposure for 72 days, activities/levels of gst, gpx and gsh were significantly higher than in corresponding control groups following exposure to 3.000 mg/l sds and sdbs. activities of sod and cat were significantly inhibited by experimental sdbs (except cat in 0.100mg/l group), but not by sds. statistical analysis of enzyme activi ... | 2010 | 20045192 |
| genotyping of hepatitis a virus detected in bivalve shellfish in galicia (nw spain). | hepatitis a virus (hav) represents a significant public health problem due to its high persistence in the environment and its transmission through contaminated water and food. bivalve shellfish are filter feeders that can bioaccumulate human pathogens found in contaminated waters, their consumption being a potential cause of hepatitis a outbreaks. in this work, cultured and wild bivalve shellfish from the ría de vigo (galicia, nw spain) were analysed for the presence and genotyping of hav. a tot ... | 2010 | 20057087 |
| acute effects of benzo[a]pyrene on digestive gland enzymatic biomarkers and dna damage on mussel mytilus galloprovincialis. | in the present study, mussel (mytilus galloprovincialis) digestive gland biotransformation and detoxification responses to acute exposure to the polycyclic aromatic hydrocarbon benzo[a]pyrene (b[a]p) were investigated. mussels were exposed to a sublethal dose of b[a]p (75 nm; 19 microg/l per animal) for 24, 48 and 72h. the following biological responses were measured in the digestive gland tissues: (1) b[a]p hydroxylase (bph) activity, as phase i biotransformation parameter; (2) glutathione s-tr ... | 2010 | 20071027 |
| amino acids from mytilus galloprovincialis (l.) and rapana venosa molluscs accelerate skin wounds healing via enhancement of dermal and epidermal neoformation. | wound healing consists of re-epithelialization, contraction and formation of granulation and scar tissue. amino acids from proteins are involved in these events, but their exact roles are not well understood. the present study was undertaken to investigate the anti-inflammatory effects of some amino acids from two molluscs, mytilus galloprovincialis (l.) (mediterranean mussel) and rapana venosa (hard shell-clam) employed in induced skin burn injuries in wistar rats. the treatment was evaluated i ... | 2010 | 20087635 |
| occurrence and distribution of metals in mussels from the cantabrian coast. | from february 2004 to april 2006, the concentrations of more than 10 metals in mussels (mytilus galloprovincialis) were measured in 10 stations from galicia to the basque country in 8 different times of sampling. the mean highest concentrations are found for zn [226.8 microg/g, dry weight (d.w.)] followed by mn, as, cu, and co (between 10 and 25 microg/g d.w.). lower mean concentrations are found for se, cr, pb, v, and ni (between 2 and 8 microg/g d.w.) and the lowest are for cd, sn, hg, and sb ... | 2010 | 20143053 |
| cytotoxic effect of palytoxin on mussel. | palytoxin is a large and complex polyhydroxylated molecule with potent neurotoxic activity. dinoflagellates from the ostreopsis genera were demonstrated to be producers of this compound and analogues. even though initially palytoxin appearance was restricted to tropical areas, the recent occurrence of ostreopsis outbreaks in mediterranean sea point to a worldwide dissemination probably related to climatic change. those dinoflagellates can bioaccumulate in shellfish, especially in filter-feeding ... | 2010 | 20206198 |
| acute toxicity of a shoreline cleaner, cytosol, mixed with oil and ecological risk assessment of its use on the galician coast. | the application of embryo-larval bioassay with the purple sea urchin paracentrotus lividus and the mussel mytilus galloprovincialis at 48 hours, and with neonates of the mysid siriella armata at 96 hours, was used to evaluate the acute toxicities of the following preparations: (1) the shoreline cleaning agent cytosol; (2) the water-accommodated fraction of cytosol plus a light crude oil; and (3) the runoff from a pilot-scale treatment with cytosol of a rocky coastal substrate impregnated with re ... | 2010 | 20217060 |
| norovirus in bivalve molluscs: a study of the efficacy of the depuration system. | noroviruses are the most common viral agents of acute gastroenteritis in humans and are often associated with the consumption of either fresh or undercooked live bivalve molluscs. the aim of the study was to evaluate the efficacy of the water depuration systems in the presence of norovirus contamination a total of 96 shellfish samples was examined by reverse transcriptase-polymerase chain reaction, as follows: 58 mussel samples (mytilus galloprovincialis), 35 manila clam samples (tapes decussatu ... | 2009 | 20391417 |
| an ecotoxicological approach with transplanted mussels (mytilus galloprovincialis) for assessing the impact of tyre reefs immersed along the nw mediterranean sea. | tyre artificial reefs were deposited in a marine protected area (vallauris-golfe juan bay, france) located along the nw mediterranean coast, during the early 80's. the potential toxic effects of the tyre artificial reefs were investigated using transplantation of marine mussels, mytilus galloprovincialis, to stations located above tyre blocks (st1, st2) or reference site (str). mussels transplanted to different stations presented the following sequence of mortality: st1 > st2 > str. principal co ... | 2010 | 20400174 |
| targets and effects of yessotoxin, okadaic acid and palytoxin: a differential review. | in this review, we focus on processes, organs and systems targeted by the marine toxins yessotoxin (ytx), okadaic acid (oa) and palytoxin (ptx). the effects of ytx and their basis are analyzed from data collected in the mollusc mytilus galloprovincialis, the annelid enchytraeus crypticus, swiss cd1 mice and invertebrate and vertebrate cell cultures. oa and ptx, two toxins with a better established mode of action, are analyzed with regard to their effects on development. the amphibian xenopus lae ... | 2010 | 20411120 |
| heavy metals in mussels (mytilus galloprovincialis) from marmara sea, turkey. | marmara sea is one of the main catching areas of mussels (mytilus galloprovincialis) in turkey, and a significant portion of the harvest has been exported mainly to european countries. in this study, zn, cu, cd, hg, and pb in mussels from ten catching areas in marmara sea were analyzed to investigate health risks associated with consuming mussels. mercury was not detected (<0.15 ppb) in any of the samples. the highest concentrations of cu and cd were 3.473 and 0.740 mg kg(-1) (wet weight, ww), r ... | 2011 | 20490711 |
| specificity of anti-vibrio immune response through p38 mapk and pkc activation in the hemocytes of the mussel mytilus galloprovincialis. | in mussel (mytilus sp.) hemocytes, differential functional responses to injection with different types of live and heat-killed vibrio species have been recently demonstrated. in this work, responses of mytilus hemocytes to heat-killed vibrio splendidus lgp32 and the mechanisms involved were investigated in vitro and the results were compared with those obtained with vibrio anguillarum (atcc 19264). adhesion of hemocytes after incubation with bacteria was evaluated by flow cytometry: both total h ... | 2010 | 20493193 |
| effects of vibrio challenge on digestive gland biomarkers and antioxidant gene expression in mytilus galloprovincialis. | in bivalve molluscs, responses to bacterial infection have been largely characterized in terms of both functional responses and gene expression in the immune cells, the hemocytes. the effects of bacterial challenge at the tissue level, where bacterial infection may cause stressful conditions, have not been so far specifically investigated. biomarkers are widely utilised to evaluate the health status of bivalves, from the molecular to the organism level, in response to both natural and anthropoge ... | 2010 | 20601119 |
| biomonitoring of organochlorine compounds and trace metals along the eastern adriatic coast (croatia) using mytilus galloprovincialis. | the paper presents the results of monitoring trace organic contaminants (17 pcbs and 7 ocps) and 6 trace metals (cd, cr, cu, hg, pb and zn) in the coastal waters of the eastern adriatic in 2006 using mytilus galloprovincialis as indicator species. ocps were found in all samples (0.07-14.3 ng g(-1)d.wt.), while in positive samples concentrations of pcbs ranged between 0.16 and 20.5 ng g(-1)d.wt. the α-hch/γ-hch and dde/ddt ratios indicated recent input of γ-hch and ddt. spatial distributions of h ... | 2010 | 20696441 |
| morphological and molecular analysis of metacercariae of diphtherostomum brusinae (stossich, 1888) stossich, 1903 from a new bivalve host mytilus galloprovincialis. | the digenean trematode diphtherostomum brusinae (stossich, 1888) stossich, 1903 presents a complex life cycle that may involve more than one intermediate host. the present study represents the first description of the metacercariae from d. brusinae infecting the labial palps of a new intermediate host, mytilus galloprovincialis, in the aveiro estuary, portugal. the morphology of this parasitic stage was studied by light (lm) and scanning electron microscopy (sem) and some differences were report ... | 2010 | 20701832 |
| food contaminant analysis at ultra-high mass resolution: application of hybrid linear ion trap - orbitrap mass spectrometry for the determination of the polyether toxins, azaspiracids, in shellfish. | the biotoxins, azaspiracids (azas), from marine phytoplankton accumulate in shellfish and affect human health by causing severe gastrointestinal disturbance, diarrhea, nausea and vomiting. specific and sensitive methods have been developed and validated for the determination of the most commonly occurring azaspiracid analogs. an ltq orbitrap mass spectrometer is a hybrid instrument that combines linear ion trap (lit) mass spectrometry (ms) with high-resolution fourier transform (ft) ms and this ... | 2010 | 20872629 |
| investigation of toxoplasma gondii presence in farmed shellfish by nested-pcr and real-time pcr fluorescent amplicon generation assay (flag). | to evaluate the presence of toxoplasma gondii in edible farmed shellfish, 1734 shellfish specimens i.e., 109 crassostrea gigas (6 pools), 660 mytilus galloprovincialis (22 pools), 804 tapes decussatus (28 pools) and 161 tapes philippinarum (6 pools), were collected from the varano lagoon (apulia, italy). shellfish from 62 pools were subjected to two molecular techniques: a nested-pcr assay, and a fluorescent amplicon generation (flag) real-time pcr assay, both based on the multi-copy b1 target, ... | 2010 | 20920501 |
| isolation of a ck2α subunit and the holoenzyme from the mussel mytilus galloprovincialis and construction of the ck2α and ck2β cdnas. | protein kinase ck2 is a ubiquitous, highly pleiotropic, and constitutively active phosphotransferase that phosphorylates mainly serine and threonine residues. ck2 has been studied and characterized in many organisms, from yeast to mammals. the holoenzyme is generally composed of two catalytic (α and/or α') and two regulatory (β) subunits, forming a differently assembled tetramer. the free and catalytically active α/α' subunits can be present in cells under some circumstances. we present here the ... | 2010 | 20922551 |
| domoic acid--a new toxin in the croatian adriatic shellfish toxin profile. | this is the first study that presents concentrations of domoic acid detected in the whole shellfish tissue from breeding and harvesting areas along the croatian coast of the adriatic sea during the period 2006 to 2008. shellfish sample analyses after sax cleaning procedures, using a uv-dad-hplc system, showed the presence of domoic acid in four species. the most prevalent of those species were the blue mussel (mytilus galloprovincialis), followed by european flat oyster (ostrea edulis), mediterr ... | 2010 | 20938398 |
| a new gene family of single fibrinogen domain lectins in mytilus. | in molluscs haemolymph lectins bearing fibrinogen-like domain (frep) act as immune pattern-recognition receptors. a full-length cdnas of mytfrep1 and mytfrep2 cloned from haemocytes of blue mussel mytilus edulis encoded putative polypeptides of 230 and 241 amino acids. both polypeptides consist of signal peptide and c-terminal fibrinogen-like domain. immune functions of these molecules may be extrapolated from the close-related and functionally characterized lectin aifrep from bay scallop, argope ... | 2010 | 20951811 |
| source and impact of lead contamination on δ-aminolevulinic acid dehydratase activity in several marine bivalve species along the gulf of cadiz. | coastal areas and estuaries are particularly sensitive to metal contamination from anthropogenic sources and in the last few decades the study of space-time distribution and variation of metals has been extensively researched. the gulf of cadiz is no exception, with several rivers draining one of the largest concentrations of sulphide deposits in the world, the iberian pyrite belt (ipb). of these rivers, the guadiana, one of the most important in the iberian peninsula, together with smaller rive ... | 2010 | 20965582 |
| differential protein expression in two bivalve species; mytilus galloprovincialis and corbicula fluminea; exposed to cylindrospermopsis raciborskii cells. | the cyanobacteria cylindrospermopsis raciborskii is considered a threat to aquatic organisms due to the production of the toxin cylindrospermopsin (cyn). despite the numerous reports evidencing the toxic effects of c. raciborskii cells and cyn in different species, not much is known regarding the toxicity mechanisms associated with this toxin and the cyanobacteria. in this work, a proteomics approach based in the two-dimensional gel electrophoresis and mass spectrometry was used to study the eff ... | 2010 | 20970860 |
| [thin filaments of molluscan catch muscles contain a calponin-like protein]. | a novel 40 kda protein was detected in native thin filaments from catch muscles of the mussel crenomytilus grayanus. the maldy-tof analysis of the protein showed a 40% homology with the calponin-like protein from the muscle of mytilus galloprovincialis (45 kda), which has a 36% homology with smooth muscle calponin from chicken gizzard (34 kda). the amount of the calponin-like protein in thin filaments depends on isolation conditions and varies from the complete absence to the presence in amounts ... | 2010 | 21033343 |
| comparison of the 2000 and 2005 spatial distributions of heavy metals in wild mussels from the north-atlantic spanish coast. | spatial distributions of cd, cu, hg, pb and zn in mytilus galloprovincialis in 41 sampling sites in the north-atlantic spanish coast were monitored in 2000 and 2005 to assess the coastal environmental pollution. a pool of mussel soft tissue was prepared using 50 or more individuals representing the available size range. samples were grounded, homogenised and quantified by atomic absorption spectrometry. quality of the chemical analyses was assessed by participation in periodic interlaboratory ex ... | 2010 | 21095005 |
| occurrence of giardia cysts in mussels (mytilus galloprovincialis) destined for human consumption. | between january and june 2004, a total of 200 nondepurated mussel samples of the galician coast (northwest spain) were examined for giardia cysts with a direct immunofluorescence antibody test. giardia cysts were found in mussels from all of the estuaries studied, with an overall rate of contamination of 41.5%. there was relation between the presence of giardia cysts, the microbiological contamination (expressed as most probable number of escherichia coli) detected in the samples, and the harves ... | 2005 | 21132982 |
| comparison of antibacterial activity in the hemolymph of marine bivalves from galicia (nw spain). | a screening study of in vitro antibacterial activity was conducted in marine bivalves with economical importance and widespread along the coast of galicia (nw spain). hemocyte lysate supernatant (hls) and plasma of mytilus galloprovincialis, ostrea edulis, crassostrea gigas, ruditapes decussatus, ruditapes philippinarum, and cerastoderma edule were incubated with vibrio splendidus and micrococcus sp. hls and plasma for all the species demonstrated antibacterial activity, and c. edule had the hig ... | 2010 | 21134379 |
| the β-blocker propranolol affects camp-dependent signaling and induces the stress response in mediterranean mussels, mytilus galloprovincialis. | widespread occurrence of pharmaceuticals is reported in aquatic systems, posing concerns for the health of aquatic wildlife and a theoretical risk to humans. a recent concept was developed for the identification of highly active compounds amongst the environmental pharmaceuticals, based on their mode of action, the homology between human targets and possible targets in the environment, and the importance of the affected pathway for the target species. in line with this approach, this study inves ... | 2010 | 21216339 |
| lipophilic toxin profile in mytilus galloprovincialis during episodes of diarrhetic shellfish poisoning (dsp) in the n.e. adriatic sea in 2006. | dinophysis spp. blooms and related shellfish toxicity events of diarrhetic shellfish poisoning (dsp) have been the most reported toxicity event through the croatian national monitoring program. with the aim to characterize the dsp toxin profile in shellfish farmed in croatia, for the first time a complete analysis of the toxin profile of croatian mussels has been carried out using the lc-ms/ms technique. the obtained results showed okadaic acid (oa) as the main toxin contaminating croatian musse ... | 2011 | 21258296 |
| insights into the innate immunity of the mediterranean mussel mytilus galloprovincialis. | sessile bivalves of the genus mytilus are suspension feeders relatively tolerant to a wide range of environmental changes, used as sentinels in ecotoxicological investigations and marketed worldwide as seafood. mortality events caused by infective agents and parasites apparently occur less in mussels than in other bivalves but the molecular basis of such evidence is unknown. the arrangement of mytibase, interactive catalogue of 7,112 transcripts of m. galloprovincialis, offered us the opportunit ... | 2011 | 21269501 |
| application of a battery of biomarkers in mussel digestive gland to assess long-term effects of the prestige oil spill in galicia and bay of biscay: tissue-level biomarkers and histopathology. | in order to assess the biological effects of the prestige oil spill (pos), mussels, mytilus galloprovincialis, were collected in 22 localities along the north coast of the iberian peninsula over 3 years (april 2003-april 2006). different tissue-level biomarkers including cell type composition (volume density of basophilic cells, vv(bas)) in digestive gland epithelium, structural changes of digestive alveoli (mean luminal radius to mean epithelial thickness, mlr/met) and histopathological alterat ... | 2011 | 21290065 |
| spatial and temporal variability of ²¹°po and ²¹°pb in mussels (mytilus galloprovincialis) at the turkish coast of the aegean sea. | in this study, the activity concentrations of ²¹°po and ²¹°pb were determined in mussel samples (mytilus galloprovincialis) collected from the turkish coast of the aegean sea. the samples were collected seasonally for a period of two years (2004-2006) at six coastal stations (çanakkale, dikili, foça, çesme, didim, bodrum). mussels were separated into several groups according to their size (1-4,4-6,>6 cm). the results showed that ²¹°po concentrations in mussels varied between 53±4 and 1960±60 bq ... | 2011 | 21310460 |
| antioxidant and lipid peroxidation responses in mytilus galloprovincialis exposed to mixtures of benzo(a)pyrene and copper. | this study aimed to assess the antioxidant system potential and lipid peroxidative effects, in the gill and digestive gland of mytilus galloprovincialis exposed to individual and binary mixtures of benzo(a)pyrene (bap) and cu for 7 days. data demonstrated that in mussels exposed to bap antioxidant enzymes (catalase--cat, total glutathione peroxidase--tgpx, glutathione s-transferase--gst and glutathione reductase--gr) and lipid peroxidation (lpo) increased in the gill. on the contrary, in the dig ... | 2011 | 21354328 |
| a large scale survey of trace metal levels in coastal waters of the western mediterranean basin using caged mussels (mytilus galloprovincialis). | a large scale study of trace metal contamination (hg, cd, pb and ni) by means of caged mussels mytilus galloprovincialis was undertaken along the coastal waters of the western mediterranean sea within the context of the mytilos project. individual mussels from an homogeneous population (shell size 50 -¦ 5 mm) obtained from an aquaculture farm were consecutively caged and deployed at 123 sites located in the albor+ín, north-western, south-western and tyrrhenian sub-basins for 12 weeks (april-july ... | 2011 | 21384032 |
| permanent genetic resources added to molecular ecology resources database 1 december 2010-31 january 2011. | this article documents the addition of 238 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: alytes dickhilleni, arapaima gigas, austropotamobius italicus, blumeria graminis f. sp. tritici, cobitis lutheri, dendroctonus ponderosae, glossina morsitans morsitans, haplophilus subterraneus, kirengeshoma palmata, lysimachia japonica, macrolophus pygmaeus, microtus cabrerae, mytilus galloprovincialis, pallisentis (neosentis) celatus, ... | 2011 | 21457476 |
| some microbial characteristics of mussels (mytilus galloprovincialis) in coastal city area. | purpose: the aim of this work was to determine some microbial characteristics of mytilus galloprovincialis harvested from four stations, located in izmir coastal area (eastern aegean sea, turkey). the area along the izmir coast is densely populated, and it includes extensive agricultural lands, industrial and domestic discharge. shellfish contamination from sewage-polluted waters is very important for public health. materials and methods: mussel samples were collected by scuba equipment. while, ... | 2011 | 21475981 |
| an rflp assay to determine if mytilus galloprovincialis lmk. (mytilidae; bivalvia) is of northern or southern hemisphere origin. | mytilus galloprovincialis is one of three smooth shelled blue mussel species belonging to the mytilus edulis species complex. naturally occurring and introduced populations of m. galloprovincialis are widely distributed throughout many regions of the globe. mytilus galloprovincialis includes morphologically indistinguishable northern and southern hemisphere mtdna lineages that have been separated for ∼1 my. to distinguish recently introduced northern m. galloprovincialis from resident southern m ... | 2010 | 21565061 |
| detection of the oyster herpesvirus in commercial bivalve in northern california, usa: conventional and quantitative pcr. | the ostreid herpesvirus (oshv-1) and related oyster herpesviruses (oshv) are associated with world-wide mortalities of larval and juvenile bivalves. to quantify oshv viral loads in mollusc tissues, we developed a sybr green quantitative pcr (qpcr) based on the a-region of the oshv-1 genome. reaction efficiency and precision were demonstrated using a plasmid standard curve. the analytical sensitivity is 1 copy per reaction. we collected crassostrea gigas, c. sikamea, c. virginica, ostrea edulis, ... | 2011 | 21648239 |
| detection of vibrio parahaemolyticus in mediterranean mussels (mytilus galloprovincialis) in slovenia. | the aim of this study was to determine the prevalence of vibrio parahaemolyticus in shellfish samples harvested along the slovenian coast. shellfish samples of mediterranean mussels (mytilus galloprovincialis) were collected along the slovenian coast at four locations (se─ìa, piran, strunjan and debeli rti─ì) between 2006 and 2008. samples were examined and analysed for the presence of v. parahaemolyticus by conventional and molecular methods. the presence of vibrio in the samples was examined b ... | 2011 | 21665569 |
| identification of octopine dehydrogenase from mytilus galloprovincialis. | a cdna encoding the putative octopine dehydrogenase (ocdh) from the mussel mytilus galloprovincialis was cloned and sequenced. the complete coding region was expressed in the bacteria escherichia coli and the recombinant protein was purified. the m. galloprovincialis ocdh appears to have the highest affinity for the amino acid substrate l-arginine (88.22%), compared to l-alanine (9.04%) and glycine (2.74%). this enzyme showed no activity when taurine or ß-alanine was used as substrate. these dat ... | 2011 | 21791249 |
| a multi-biomarker approach in cross-transplanted mussels mytilus galloprovincialis. | the present work integrates the active biomonitoring (abm) concept in mussels mytilus galloprovincialis from the south coast of portugal transplanted during 28 days between two sites with different sources of contamination, and vice versa, in order to assess biological effects in these mussels. for that purpose a multibiomarker approach was used. the suit of biomarkers indicative of metal contamination were metallothioneins (mt) and the enzyme d-aminolevulinic acid dehydratase (alad), for organi ... | 2011 | 21769590 |
| non-steroidal anti-inflammatory drug (nsaid) ibuprofen distresses antioxidant defense system in mussel mytilus galloprovincialis gills. | active pharmaceutical ingredients (apis) are presently considered an emergent class of environmental contaminants. ibuprofen (ibu) is one of the most applied non-steroidal anti-inflammatory drugs (nsaids) in the world. several authors report the occurrence of ibu in influents and effluents of waste water treatment plants (wwtps), surface, river and public tap water in numerous countries. however, very little is known about the risks and chronic effects of ibu exposure in non-target organisms. th ... | 2011 | 21767472 |
| efficiency of butyl rubber sorbent to remove the pah toxicity. | large amounts of polycyclic aromatic hydrocarbons (pahs) have been released to the marine environment as a result of oil spills and from other sources including wastewaters, surface runoff, industrial processes, atmospheric deposition, biosynthesis, and natural events such as forest fires. pahs have been known to affect a variety of biological processes and can be potent cell mutagens/carcinogens and toxic. in this study, pah toxicity removal was investigated by using a novel macroporous butyl r ... | 2011 | 21714631 |
| a case study of trace metals in suspended particulate matter and biota before wastewater treatment plant from the izmir bay, turkey. | the concentrations of trace metals (cu, zn, mn, ni, and fe) from suspended particulate matter (spm) and biota in izmir bay (eastern aegean sea) were studied in order to evaluate the environmental impact of the anthropogenic metals before building of wastewater treatment plant. spm samples were collected in wet and dry periods from izmir bay. metal concentrations in spm (cu, 0.36-2.19; mn, 0.07-11.3; ni, 0.43-7.81; zn, 7.33-269; fe, 1.00-266-á++gdm(ôçë-ôçë3)) were comparable to those reported for ... | 2011 | 21701893 |
| bioaccumulation of algal toxins and changes in physiological parameters in mediterranean mussels from the north adriatic sea (italy). | the northwestern adriatic sea is a commercially important area in aquaculture, accounting for about 90% of the italian mussel production, and it was subjected to recurring cases of mussel farm closures due to toxic algae poisoning. a spatial and temporal survey of four sites along the north adriatic sea coasts of emilia romagna (italy) was undertaken to study the possible impairments of physiological parameters in mytilus galloprovincialis naturally exposed to algal toxins. the sites were select ... | 2011 | 21695759 |
| MIF from mussel: Coding sequence, phylogeny, polymorphism, 3D model and regulation of expression. | Three macrophage migration inhibitory factor (MIF)-related sequences were identified from a Mytilus galloprovincialis EST library. The consensus sequence included a 5'-UTR of 32 nucleotides, the complete ORF of 345 nucleotides, and a 3'-UTR of 349 nucleotides. As for other MIFs, M. galloprovincialis ORF does not include any signal or C-terminus extensions. The translated sequence of 115 amino acids possesses a molecular mass of 12,681.4, a pI of 6.27 and a stability index of 21.48. Its 3D struct ... | 2011 | 22085783 |
| gene expression specificity of the mussel antifungal mytimycin (mytm). | we previously reported the nucleotide sequences and diversity of mytimycin (mytm) from the mediterranean mussel, mytilus galloprovincialis. using real-time pcr (q-pcr), we observed that the mytm gene was mainly expressed in circulating hemocytes and to a less extent in the mantle. in vivo challenge with bacteria or with the yeast, candida albicans, did not increase the expression as measured by q-pcr in hemocytes. by contrast, injection of the filamentous fungus, fusarium oxysporum, induced a su ... | 2012 | 22037382 |
| cloning and localization of mcdef, a defensin from manila clams (ruditapes philippinarum). | a defensin-like peptide was previously detected in hemocytes of manila clams (ruditapes philippinarum). in the current study, we cloned and characterized this defensin, designated mcdef. cloning produced a full-length gene sequence of 201 bp predicted to encode a 66-amino-acid precursor protein maturing to a 44-amino-acid residue. amino acid sequence analysis showed that mcdef is similar to defensins from marine mollusks and ticks. phylogenetic analysis suggested that mcdef is closely related to ... | 2012 | 21945146 |
| Massively parallel amplicon sequencing reveals isotype-specific variability of antimicrobial peptide transcripts in Mytilus galloprovincialis. | Effective innate responses against potential pathogens are essential in the living world and possibly contributed to the evolutionary success of invertebrates. Taken together, antimicrobial peptide (AMP) precursors of defensin, mytilin, myticin and mytimycin can represent about 40% of the hemocyte transcriptome in mussels injected with viral-like and bacterial preparations, and unique profiles of myticin C variants are expressed in single mussels. Based on amplicon pyrosequencing, we have ascert ... | 2011 | 22087233 |
| In vitro evaluation of antibacterial activity of Asparagopsis taxiformis from the Straits of Messina against pathogens relevant in aquaculture. | Ethanol extracts of Asparagopsis taxiformis collected from the Straits of Messina (Italy) were screened for antibacterial activity against pathogenic shellfish and fish bacteria previously isolated from local marine and brackish environments. Genetic labelling by DNA barcoding allowed us to identify the algal population as a biogeographical strain conspecific to A. taxiformis. The extract obtained in May showed the broadest antibacterial activity against all tested pathogenic bacteria, especiall ... | 2012 | 22078751 |
| carbamazepine-mediated pro-oxidant effects on the unicellular marine algal species dunaliella tertiolecta and the hemocytes of mussel mytilus galloprovincialis. | this study investigates the pro-oxidant behavior of the antiepileptic drug carbamazepine (cbz) on the marine algal species dunaliella tertiolecta and the immune defense-related hemocytes of mussel mytilus galloprovincialis. a phytotoxicity test, performed in a first step, showed a significant inhibition of the growth rate and the chlorophyll alpha (chl-α) content in algae after exposure for 24 h to different concentrations of cbz (1-200 mg l(-1)). on the other hand, the increased levels of lipid ... | 2013 | 23912321 |
| from broad-spectrum biocides to quorum sensing disruptors and mussel repellents: antifouling profile of alkyl triphenylphosphonium salts. | 'onium' compounds, including ammonium and phosphonium salts, have been employed as antiseptics and disinfectants. these cationic biocides have been incorporated into multiple materials, principally to avoid bacterial attachment. in this work, we selected 20 alkyl-triphenylphosphonium salts, differing mainly in the length and functionalization of their alkyl chains, in fulfilment of two main objectives: 1) to provide a comprehensive evaluation of the antifouling profile of these molecules with re ... | 2015 | 25897858 |
| two goose-type lysozymes in mytilus galloprovincialis: possible function diversification and adaptive evolution. | two goose-type lysozymes (designated as mgglyz1 and mgglyz2) were identified from the mussel mytilus galloprovincialis. mgglyz1 mrna was widely expressed in the examined tissues and responded sensitively to bacterial challenge in hemocytes, while mgglyz2 mrna was predominately expressed and performed its functions in hepatopancreas. however, immunolocalization analysis showed that both these lysozymes were expressed in all examined tissues with the exception of adductor muscle. recombinant mggly ... | 2012 | 23028813 |
| a novel c-type lysozyme from mytilus galloprovincialis: insight into innate immunity and molecular evolution of invertebrate c-type lysozymes. | a c-type lysozyme (named as mgclyz) gene was cloned from the mussel mytilus galloprovincialis. blast analysis indicated that mgclyz was a salivary c-type lysozyme which was mainly found in insects. the nucleotide sequence of mgclyz was predicted to encode a polypeptide of 154 amino acid residues with the signal peptide comprising the first 24 residues. the deduced mature peptide of mgclyz was of a calculated molecular weight of 14.4 kd and a theoretical isoelectric point (pi) of 8.08. evolution ... | 2013 | 23818979 |
| pyrosequencing of mytilus galloprovincialis cdnas: tissue-specific expression patterns. | mytilus species are important in marine ecology and in environmental quality assessment, yet their molecular biology is poorly understood. molecular aspects of their reproduction, hybridisation between species, mitochondrial inheritance, skewed sex ratios of offspring and adaptation to climatic and pollution factors are priority areas. | 2010 | 20111607 |
| histone h2a (h2a.x and h2a.z) variants in molluscs: molecular characterization and potential implications for chromatin dynamics. | histone variants are used by the cell to build specialized nucleosomes, replacing canonical histones and generating functionally specialized chromatin domains. among many other processes, the specialization imparted by histone h2a (h2a.x and h2a.z) variants to the nucleosome core particle constitutes the earliest response to dna damage in the cell. consequently, chromatin-based genotoxicity tests have been developed in those cases where enough information pertaining chromatin structure and dynam ... | 2012 | 22253857 |
| the mirna biogenesis in marine bivalves. | small non-coding rnas include powerful regulators of gene expression, transposon mobility and virus activity. among the various categories, mature micrornas (mirnas) guide the translational repression and decay of several targeted mrnas. the biogenesis of mirnas depends on few gene products, essentially conserved from basal to higher metazoans, whose protein domains allow specific interactions with dsrna. here, we report the identification of key genes responsible of the mirna biogenesis in 32 b ... | 2016 | 26989613 |
| new insights into the apoptotic process in mollusks: characterization of caspase genes in mytilus galloprovincialis. | apoptosis is an essential biological process in the development and maintenance of immune system homeostasis. caspase proteins constitute the core of the apoptotic machinery and can be categorized as either initiators or effectors of apoptosis. although the genes encoding caspase proteins have been described in vertebrates and in almost all invertebrate phyla, there are few reports describing the initiator and executioner caspases or the modulation of their expression by different stimuli in dif ... | 2011 | 21347300 |
| aeromonas spp. simultaneously harbouring bla(ctx-m-15), bla(shv-12), bla(per-1) and bla(fox-2), in wild-growing mediterranean mussel (mytilus galloprovincialis) from adriatic sea, croatia. | aeromonas species are becoming renowned as emerging pathogens by increasingly giving rise to a wide spectrum of food and waterborne infections in humans. another worrisome feature of aeromonads is the growing frequency of antibiotic resistance as a consequence of their prominent diversity in terms of resistance determinants. this study aimed at determining the antimicrobial resistance pattern, prevalence and characterization of acquired β-lactamases, including extended-spectrum-β-lactamases (esb ... | 2013 | 23973842 |
| genetic diversity of arcobacter isolated from bivalves of adriatic and their interactions with mytilus galloprovincialis hemocytes. | the human food-borne pathogens arcobacter butzleri and a. cryaerophilus have been frequently isolated from the intestinal tracts and fecal samples of different farm animals and, after excretion, these microorganisms can contaminate the environment, including the aquatic one. in this regard, a. butzleri and a. cryaerophilus have been detected in seawater and bivalves of coastal areas which are affected by fecal contamination. the capability of bivalve hemocytes to interact with bacteria has been ... | 2017 | 27650799 |
| detection of arcobacter spp. in mytilus galloprovincialis samples collected from apulia region. | the aim of the study was to evaluate the occurrence of arcobacter spp. in 20 samples of mytilus galloprovincialis purchased at fish markets in apulia region. the detection of arcobacter spp. was performed, after selective enrichment, on modified charcoal cefoperazone deoxycholate (mccd) agar supplemented with cefoperazone, amphotericin b and teicoplanin (cat). in 6 out of the 20 tested samples the presence of arcobacter spp. was found and confirmed by genus-based polymerase chain reaction. all t ... | 2015 | 27800382 |
| bioaccumulation experiments in mussels contaminated with the food-borne pathogen arcobacter butzleri: preliminary data for risk assessment. | the aim of this study was to evaluate, at a laboratory scale, the ability of this microorganism to grow in seawater and bioaccumulate in mussels (mytilus galloprovincialis) maintained in constantly aerated tanks, containing twenty litres of artificial seawater. three concentrations of a. butzleri lmg 10828(t) were tested (about 5 × 10⁶ cfu/ml, 5 × 10⁴ cfu/ml, and 5 × 10² cfu/ml). following contamination, enumeration of a. butzleri was performed from water and mussels each day, for up to 96 h. th ... | 2013 | 24102050 |
| ecotoxicological potential of antibiotic pollution-industrial wastewater: bioavailability, biomarkers, and occurrence in mytilus galloprovincialis. | environmental pollution by pharmaceutical residues has become a major problem in many countries worldwide. however, little is known about the concentrations of pharmaceuticals in water sources in tunisia. residues in the natural environment have been of increasing concern due to their impact on bacteria resistance development and toxicity to natural communities and ultimately to public health. in this work, we collected the wastewater sample from a pharmaceutical industry, which specializes in t ... | 2016 | 27109112 |
| occurrence and antibiotic susceptibility profiles of burkholderia cepacia complex in coastal marine environment. | during an environmental study of bacterial resistance to antibiotics in coastal waters of the kaštela bay, adriatic sea, croatia, 47 burkholderia cepacia complex (bcc) isolates were recovered from seawater and mussel (mytilus galloprovincialis) samples. all isolates showed multiple antibiotic resistance. among the isolates, two burkholderia cenocepacia isolates produced chromosomally encoded tem-116 extended-spectrum β-lactamase (esbl). analysis of outer membrane proteins revealed that decreased ... | 2012 | 22428949 |
| profile and multidrug resistance determinants of chryseobacterium indologenes from seawater and marine fauna. | the aim of this study was to investigate the prevalence and genetic basis of multidrug resistance in chryseobacterium indologenes from seawater and marine invertebrates used for human consumption, in kaštela bay, adriatic sea, croatia. out of 16 samples of seawater, mediterranean mussel (mytilus galloprovincialis lam.), rayed mediterranean limpets (patella caerulea l.) and purple sea urchins (paracentrotus lividus lam.) collected, 15 were positive for c. indologenes. in total, 41 isolates were r ... | 2013 | 23132253 |
| occurrence of toxigenic clostridium difficile in edible bivalve molluscs. | clostridium difficile is an anaerobic bacterium commonly considered to be responsible for antibiotic-associated gastrointestinal diseases, ranging from diarrhea of varying severity to pseudomembranous colitis. the aim of this study was to assess the occurrence of c. difficile in marine edible bivalve molluscs, which, as filter feeding organisms, are able to accumulate particles suspended in water, including microorganisms. samples of mytilus galloprovincialis, tapes philippinarum, and venus verr ... | 2012 | 22608238 |
| multiplex pcr for the detection and quantification of zoonotic taxa of giardia, cryptosporidium and toxoplasma in wastewater and mussels. | giardia duodenalis, cryptosporidium parvum and toxoplasma gondii are important parasitic protists linked to water- and food-borne diseases. the accurate detection of these pathogens is central to the diagnosis, tracking, monitoring and surveillance of these protists in humans, animals and the environment. in this study, we established a multiplex real-time pcr (qpcr), coupled to high resolution melting (hrm) analysis, for the specific detection and quantification of each g. duodenalis (assemblag ... | 2015 | 25591902 |
| cryptosporidium parvum genotype iia and giardia duodenalis assemblage a in mytilus galloprovincialis on sale at local food markets. | to date, there has been no study to establish the genotypic or subgenotypic identities of cryptosporidium and giardia in edible shellfish. here, we explored the genetic composition of these protists in mytilus galloprovincialis (mediterranean mussel) purchased from three markets in the city of foggia, italy, from may to december 2012. samples from the digestive glands, gills and haemolymph were tested by nested pcr, targeting dna regions within the 60 kda glycoprotein (gp60) gene of cryptosporid ... | 2014 | 24334090 |
| detection of toxoplasma gondii and cyclospora cayetanensis in mytilus galloprovincialis from izmir province coast (turkey) by real time pcr/high-resolution melting analysis (hrm). | to evaluate the presence of toxoplasma gondii and cyclospora cayetanensis in edible shellfish, a total of 795 specimens of mytilus galloprovincialis from 8 different sites on the west coast of turkey (bays of izmir and mersin) were examined. fifty-three pooled samples were created and subjected to evagreen(®) realtime pcr assay and hrm analysis for protozoans detection and oocyst quantification. seven out of 8 sites were contaminated with at least one protozoan species. twenty-one out of 53 (39. ... | 2014 | 25084654 |
| killing of vibrio cholerae and escherichia coli strains carrying d-mannose-sensitive ligands by mytilus hemocytes is promoted by a multifunctional hemolymph serum protein. | in aquatic environments, bivalve mollusks represent an important ecological niche for microorganisms. persistence of bacteria in bivalve tissues partly depends on their capacity to survive the bactericidal activity of the hemolymph due to both cellular (hemocyes) and soluble serum factors (e.g., enzymes, lectins, opsonins). the extrapallial protein (ep) present in serum of mytilus galloprovincialis (mgep) has been recently shown to work as an opsonin promoting d-mannose sensitive (ms) interactio ... | 2016 | 27041371 |
| the co-occurrence of the demosponge hymeniacidon perlevis and the edible mussel mytilus galloprovincialis as a new tool for bacterial load mitigation in aquaculture. | pollutants in marine coastal areas are mainly a consequence of anthropogenic inputs, and microorganisms often play a major role in determining the extent of this pollution. thus, practical and eco-friendly techniques are urgently required in order to control or minimise the pathogenic bacterial problem. the bacterial accumulation of mytilus galloprovincialis (lamarck 1919) in the presence or absence of another filter feeder, the demosponge hymeniacidon perlevis (montagu 1818) on sewage flowing i ... | 2016 | 26498810 |
| structural and antimicrobial features of peptides related to myticin c, a special defense molecule from the mediterranean mussel mytilus galloprovincialis. | mussels (mytilus spp.) have a large repertoire of cysteine-stabilized α,β peptides, and myticin c (mytc) was identified in some hundreds of transcript variants after in vivo immunostimulation. using a sequence expressed in italian mussels, we computed the mytc structure and synthesized the mature mytc and related peptide fragments (some of them also prepared in oxidized form) to accurately assess their antibacterial and antifungal activity. only when tested at ph 5 was the reduced mytc as well a ... | 2015 | 26444944 |
| strong underwater adhesives made by self-assembling multi-protein nanofibres. | many natural underwater adhesives harness hierarchically assembled amyloid nanostructures to achieve strong and robust interfacial adhesion under dynamic and turbulent environments. despite recent advances, our understanding of the molecular design, self-assembly and structure-function relationships of these natural amyloid fibres remains limited. thus, designing biomimetic amyloid-based adhesives remains challenging. here, we report strong and multi-functional underwater adhesives obtained from ... | 2014 | 25240674 |
| occurrence of vibrio and salmonella species in mussels (mytilus galloprovincialis) collected along the moroccan atlantic coast. | this study reports the occurrence of different vibrio and salmonella species in 52 samples of mytilus galloprovincialis collected from four sites along the atlantic coast between agadir and essaouira (anza, cap ghir, imssouane and essaouira). the level of escherichia coli (e. coli) was also determined to evaluate the degree of microbial pollution in the investigated areas. in this study three methods were used : afnor nf en iso 6579 v08-013 for salmonella spp., the provisional method routinely u ... | 2014 | 24936389 |
| rotavirus occurrence in shellfish with low levels of e. coli. | the purpose of this study was to evaluate "in field" the accumulation of virus in shellfish and compare it with the concentration of bacterial indicators. individuals of mytilus galloprovincialis were placed in two sampling station located in a contaminated coastal bay and in one control station located one kilometer offshore. the presence of rotavirus and e. coli was assessed weekly both in seawater and in shellfish samples. the rotavirus genome was detected in water, preliminarily concentrated ... | 2013 | 23897404 |
| ph-dependent solution structure and activity of a reduced form of the host-defense peptide myticin c (myt c) from the mussel mytilus galloprovincialis. | myticin c (myt c) is a highly variable host-defense peptide (hdp) associated to the immune response in the mediterranean mussel (mytilus galloprovincialis), which has shown to be active across species due to its strong antiviral activity against a fish rhabdovirus found in fish cells overexpressing this hdp. however, the potential antimicrobial properties of any synthetic analogue of myt c has not yet been analysed. thus, in this work we have synthesised the sequence of the mature peptide of myt ... | 2013 | 23880927 |
| immune responses during the larval stages of mytilus galloprovincialis: metamorphosis alters immunocompetence, body shape and behavior. | we investigated the development of the immune system during the larval stages of the mussel mytilus galloprovincialis. the ability of trochophore and veliger larvae to phagocytose foreign particles (escherichia coli and zymosan) was measured. phagocytosis was detected as early as 24 h post-fertilization (hpf) using flow cytometry and fluorescence microscopy. however, although there was a high basal production of reactive oxygen and nitrogen species (ros and nrs), the phagocytosis of zymosan did ... | 2013 | 23684811 |
| escherichia coli, salmonella spp., hepatitis a virus and norovirus in bivalve molluscs in southern italy. | european legislation has fixed microbiological, chemical and biotoxicological limits for shellfish but no limits for viruses. in the present study we report the results of an investigation on salmonella spp., escherichia coli, hepatitis a virus (hav) and norovirus (nov) contamination in 59 bivalve shellfish collected during the years 2011-2012 in southern italy. all the samples of mytilus galloprovincialis and of solen marginatus were negative for hav whereas 6.8% of them were positive for norov ... | 2013 | 23564588 |
| detection of viruses in coastal seawater using mytilus galloprovincialis as an accumulation matrix. | the purpose of this study was to demonstrate that shellfish can be used to detect enteric viruses in marine waters where they are present at very low concentrations. aqua-cultured mussels were placed in the sea just off the mouth of a drainage channel affected by human and animal faecal contamination. samples were taken from the channel, the sea and the mussels at intervals over two 4-week periods. the samples were tested to verify the presence of both rotaviruses and e. coli. rotaviruses were d ... | 2012 | 23412814 |
| sewage-exposed marine invertebrates: survival rates and microbiological accumulation. | a large number of bacteria, including agents responsible for diseases, characterise sewage-polluted seawaters. apart from standards for bathing waters and bivalve aquaculture waters, there are no general microbiological standards applicable to seawaters to help decide if bacterial pollution is within acceptable ranges. this study represents an attempt towards the issue of comparing the susceptibility of different marine invertebrates subjected to polluted seawater with a high microbial contamina ... | 2013 | 22976047 |
| toll-like receptors and myd88 adaptors in mytilus: complete cds and gene expression levels. | tlr- and myd88-related sequences have been previously investigated in mytibase and then in new transcript reads obtained by illumina technology from the mussel, mytilus galloprovincialis. based on full cds and domain organizations of virtual translations, we identified 23 toll-like receptors (tlrs) and 3 myd88 adaptors. mgtlrs can be arranged in 4 clusters according to extra-cellular lrr domain content. mgtlr-b, -i and -k were the only ones containing a multiple cysteine cluster (mcctlr), a doma ... | 2013 | 23485525 |
| individual variability of mytimycin gene expression in mussel. | the antifungal peptide mytimycin (mytm) is synthesized by hemocytes of the mediterranean mussel, mytilus galloprovincialis. in addition to sequence and gene structure diversities previously reported from pooled hemocytes, the present report focused on the expression of mytm gene in individual m. galloprovincialis, before and after challenge. within untreated mussel, mytm mrna was observed by ish in about 42% of circulating hemocytes, characterized by large, diffuse nucleus. injection with fusari ... | 2012 | 22750680 |
| prevalence of foodborne viruses in mussels in southern italy. | in this study, the prevalence of various enteric viruses in mytilus galloprovincialis (mediterranean mussel) belonging to class a and class b mollusc-harvesting areas in the campania region in southern italy was evaluated. one hundred and eight mussels were analysed using real-time reverse transcription pcr during a 2-year collection period (2014-2015) to detect the following viruses: human norovirus (genogroups i and ii), rotavirus, astrovirus, sapovirus, aichivirus, hepatitis a virus and hepat ... | 2017 | 28054332 |
| presence of hepatitis e rna in mussels used as bio-monitors of viral marine pollution. | mussels (mytilus galloprovincialis), collected from a harvesting area approved by european community regulation, were transplanted to four polluted sites located in the northwestern mediterranean area (tuscany). they were used as bio-monitors to test the quality of the marine water pollution. at different times after the transplantation, mussels were withdrawn and tested for presence of phages and enteric viruses by molecular tests. 52.4% of the transplanted mussel samples were positive for at l ... | 2012 | 22728271 |
| investigation of parasitic and viral pathogens in mussels (mytilus galloprovincialis) in the gulf of izmir, turkey. | to investigate microsporidia spp. parasite, hepatitis a virus (hav), and norovirus (nov) contamination in mussels collected from 8 stations in the inner, middle, and outer regions of the gulf of izmir. | 2016 | 27279520 |