| [oncogenic properties of human adenovirus type 12 for certain mammals]. | | 1966 | 4988196 |
| [transformation of newborn green monkey kidney cell cultures induced by adenovirus type 12]. | | 1970 | 4990486 |
| [immunological analysis of hamster sarcoma induced by adenovirus type 12]. | | 1970 | 4991659 |
| the polypeptides of adenovirus type 12. | | 1973 | 4753438 |
| susceptibility of xeroderma pigmentosum cells to chromosome breakage by adenovirus type 12. | | 1974 | 4845660 |
| genetic studies with tumorigenic adenoviruses. 3. recombination in adenovirus type 12. | | 1972 | 5058331 |
| temperature-sensitive mutants of adenovirus type 12 defective in viral dna synthesis. | ten temperature-sensitive (ts) mutants of adenovirus type 12 which produce plaques at 31 but not at 38.5 c have been isolated after mutagenesis with nitrosoguanidine or nitrous acid. the mutants have been classified into six separate complementation groups. dna-dna hybridizations have shown that at 38.5 c the ts 401 and 406 mutants of groups b and e, respectively, synthesized less than 10% of the normal level of viral dna. the two mutants were also defective in the production of late proteins at ... | 1974 | 4854616 |
| characterization of adenovirus dna in kb cells infected with adenovirus type 12. | | 1972 | 5039024 |
| [the effect of intraperitoneal or subcutaneous immunization with tumor cell membranes on the enhancement of adenovirus type 12 induced tumors in hamsters]. | | 1972 | 5055039 |
| the dna of adenovirus type 12 and its denaturation pattern. | | 1972 | 5058840 |
| [interfering activity of cold-adapted strains of adenovirus types 2 and 4 with adenovirus type 12]. | | 1972 | 5062526 |
| [complement fixing antibodies to the thermostable component of adenovirus type 12 t-antigen in the serum of cancer patients]. | | 1971 | 5137544 |
| intracellular forms of adenovirus deoxyribonucleic acid. i. evidence for a deoxyribonucleic acid-protein complex in baby hamster kidney cells infected with adenovirus type 12. | the total intracellular deoxyribonucleic acid (dna) from baby hamster kidney cells abortively infected with (3)h-adenovirus type 12 was analyzed in dye-buoyant density gradients. between 10 and 20% of the cell-associated radioactivity derived from viral dna bands in a density position which is 0.043 to 0.085 g/cm(3) higher than that of viral dna extracted from purified virions. the dna in the high-density region (hp-fraction) is almost completely absent when dna, ribonucleic acid (rna) or protei ... | 1972 | 5062681 |
| titration of adenovirus type 12 and type 18 in human embryonic kidney cell cultures. | | 1966 | 5296932 |
| the nature of tumor antigen of adenovirus type 12 and its formation in cultured cells after infection. | | 1966 | 5296935 |
| responses of calf kidney cells to human adenovirus type 12. | | 1971 | 5316575 |
| detection of adenovirus type 12 neoantigen (s) in a continuous human amnion cell line (fl) by immunofluorescence. | | 1965 | 5323559 |
| viruses and mammalian chromosomes. vi. comparative karyologic and immunofluorescent studies on syrian hamster and human amnion cells infected with human adenovirus type 12. | | 1966 | 5327955 |
| the detection of intracellular adenovirus type 12 antigens by indirect immunoferritin technique. | | 1967 | 5341938 |
| three size-classes of intracellular adenovirus deoxyribonucleic acid. | when human adenovirus type 2 or 12 infects cells, either productively or non-productively, three classes of viral deoxyribonucleic acid (dna) are found within the cells: (i) viral dna which cosediments with dna extracted from infectious adenovirions at 31.3s for adenovirus type 2 and at 29.0s for adenovirus type 12, (ii) viral dna which sediments at about 18s, and (iii) viral dna which sediments at >45s and is apparently integrated into the cellular dna. a precursor-product relationship is sugge ... | 1971 | 5105670 |
| development of tumors in hamsters by in utero injection of adenovirus type 12 into fetuses. | | 1969 | 5346347 |
| biological characterization of structural components of adenovirus type 12. | | 1969 | 5347404 |
| studies on adenovirus type 12 strain variation. i. cross-neutralization tests. | | 1969 | 5369725 |
| temperature-sensitive mutants of human adenovirus type 12. | | 1971 | 5125575 |
| the fate of the dna of adenovirus type 12 in baby hamster kidney cells. | | 1968 | 5248820 |
| [amino acid composition of ribonucleoprotein and purified t-antigen isolated from hamster tumors induced by adenovirus type 12]. | | 1970 | 5507924 |
| tumor induction by adenovirus type 12 and its target cells in the central nervous system. | | 1969 | 5345656 |
| enzymatic changes in cell cultures infected with adenovirus type 12. | | 1967 | 5598986 |
| absence of replication of the dna of adenovirus type 12 in bhk21 cells. | | 1970 | 5461848 |
| relationship between induction of thymidine kinase and potentiation of growth of h-1 virus by human adenovirus 12. | | 1970 | 5455700 |
| adenovirus type 12-rat embryo transformation system. | adenovirus type 12 (huie) inoculated into cultures of primary whole rat embryo produced foci of morphologically altered cells. the number and identification of these transformed areas was dependent upon the calcium concentration of the medium; more foci appeared in 0.1 mm than in 1.8 mm calcium. cell lines derived from these inoculated cultures did not yield infectious virus, and also were similar to cell lines derived from adenovirus type 12-induced tumors with respect to morphology, presence o ... | 1967 | 5630380 |
| recovery of infectious virus from adenovirus type-12 tumor cells. | | 1968 | 5638605 |
| reactivity of human sera with adenovirus type-12 hamster tumor antigens in the complement-fixation test. | | 1968 | 5638606 |
| attempts to find human adenovirus type-12 tumor antigens in human tumors. | | 1968 | 5638607 |
| autoradiographic studies on nuclei and chromosomes of cultured leukocytes after infection with tritium-labeled adenovirus type 12. | | 1968 | 5640821 |
| transformation of a hamster cell line by adenovirus type 12. | | 1968 | 5650922 |
| transformation of rat kidney cells by adenovirus type 12. | | 1968 | 5654764 |
| oncogenicity of mixtures of adeno-associated virus and adenovirus type 12. | | 1968 | 5659627 |
| ultrastructural study of the replication of human adenovirus type 12 in cultured cells. | | 1968 | 5660942 |
| virus and dna synthesis in x-irradiated human kidney cell cultures exposed to adenovirus type 12. | | 1968 | 5668103 |
| [symposium on the "cancer virus" 7. in vitro transformation by adenovirus type-12 and characteristics of the transformed cell]. | | 1967 | 5627339 |
| application of passive hemagglutination methods for detection of antibody to viral antigen in hamsters bearing adenovirus type 12 tumors. | | 1968 | 5692103 |
| separation of two components of adenovirus type 12 induced t-antigens with sephadex g-50. | | 1968 | 5703038 |
| genetic studies with tumorigenic adenoviruses. i. isolation of cytocidal (cyt) mutants of adenovirus type 12. | | 1968 | 5723670 |
| biochemical studies on adenovirus multiplication. 13. synthesis of virus-specific ribonucleic acid during infection with human adenovirus type 12. | the transcription of virus-specific ribonucleic acid (rna) was studied in kb cells infected with adenovirus type 12 (strain huie). viral deoxyribonucleic acid (dna) synthesis began at 12 to 15 hr after infection, and virus maturation occurred between 20 and 50 hr after infection. the rate of incorporation of (3)h-uridine into rna per infected cell was stimulated, reaching a maximum of 1.6 times that of uninfected cells at 20 hr after infection. "early" viral messenger ribonucleic acid (mrna) con ... | 1968 | 5723708 |
| human adenovirus type 12 as a "helper" for growth of h-1 virus. | | 1968 | 5742035 |
| failure to detect incorporation of host cell dna into virions of adenovirus type 12. | | 1969 | 5784049 |
| genetic studies with tumorigenic adenoviruses. ii. heterogeneity of cyt mutants of adenovirus type 12. | | 1969 | 5784054 |
| integration of the deoxyribonucleic acid of adenovirus type 12 into the deoxyribonucleic acid of baby hamster kidney cells. | in a previous report, evidence was presented that the deoxyribonucleic acid (dna) of adenovirus type 12 (ad12) is integrated by covalent linkage into the dna of baby hamster kidney cells (bhk-21 cells). these studies have been extended. the dna of ad12 and that of bhk-21 cells grown in medium containing 5-bromodeoxyuridine could be separated by equilibrium centrifugation in alkaline cscl density gradients. bhk-21 cells were infected with (3)h-labeled ad12, and the total intracellular dna was ana ... | 1970 | 5530012 |
| cytogenetical study of cells transformed in vitro by human adenovirus type 12. | | 1969 | 5789721 |
| nonproductive infection of baby hamster kidney cells (bhk21) with adenovirus type 12. | | 1969 | 5816953 |
| induction of specific chromosomal aberrations by adenovirus type 12 in human embryonic kidney cells. | nonrandom chromosomal breaks in chromosomes 1 and 17 were provoked in human embryonic kidney cells 24 hr after infection with adenovirus type 12. these chromosomal changes disappeared in persistently infected cultures. neutralization of the virus with type-specific antiviral serum prior to infection prevented the occurrence of chromosomal aberrations. no viral deoxyribonucleic acid (dna) synthesis, as determined by autoradiography, was seen in metaphases containing adenovirus type 12-induced chr ... | 1967 | 5621489 |
| disappearance of tumorigenic activity from human adenovirus type 12. | | 1965 | 5840895 |
| karyologic studies of normal cells and of adenovirus-type-12-induced tumor cells of the syrian hamster. | | 1965 | 5849591 |
| electronmicroscopic examination of kb cell cultures infected with adenovirus type 12. | | 1965 | 5857325 |
| transformation of hamster embryo cells and tumor induction in newborn hamsters by simian adenovirus sv11. | simian adenovirus, sv11, readily transformed hamster embryo cell cultures in vitro and produced tumors in vivo when inoculated into newborn hamsters. foci consisting of small, loosely attached, rounded cells could be seen as early as 7 days postinoculation. many of these cells contained several nuclei or the nucleus was multilobed. the cells grew without extensive cell to cell contact or formed small chains or clusters when passaged in vitro. this pattern of cell morphology and growth has not be ... | 1969 | 5786181 |
| [morphologic structure of adenovirus type 12]. | | 1965 | 5894907 |
| viruses and mammalian chromosomes. v. chromosome aberrations in tumors of syrian hamsters induced by adenovirus type 12. | | 1965 | 5840075 |
| isolation and analysis of adenovirus 12 sub-units from adenovirus type 12-infected cells. | | 1966 | 5968787 |
| an improved method of assay for human adenovirus type 12 (prototype strain) employing a clonal line of porcine kidney cells (brief report). | | 1966 | 5987105 |
| [effects of adenovirus type 12 on hereditary mechanism of human cells]. | | 1966 | 6010155 |
| neoplasms in hamsters induced by adenovirus type 12. | | 1967 | 6019572 |
| specific transplantation antigens of mouse sarcomas induced by adenovirus type 12. | a specific isograft resistance against three different mouse adeno 12 sarcomas was demonstrated in mice treated with four to eight doses of viable or x-ray-killed adeno 12 mouse tumors. whole-body x-ray irradiation with 350 r 24 hr previous to the test challenge did not abolish the resistance, indicating that it was due to a true anamnestic immune reaction. this was further proven by the finding that similar treatment with tumors of other origin did not induce any immunity, nor did the treatment ... | 1967 | 6020008 |
| viruses and mammalian chromosomes. vii. the persistence of a chromosomal instability in regenerating, transplanted, and cultured neoplasms induced by human adenovirus type 12 in syrian hamsters. | | 1967 | 6021516 |
| adenovirus type 12 oncogenicity in newborn hamsters. the effect of pokeweed mitogen. | | 1968 | 5681657 |
| in vitro transformation of cells of hamster brain by adenovirus type 12. | | 1967 | 6030579 |
| electron microscopic localization of adenovirus type 12 antigens. | | 1967 | 6055784 |
| effect of neonatal thymectomy on tumour induction by adenovirus type 12 in hamsters. | | 1967 | 6057758 |
| transformation in vitro of the embryonal hamster brain cells by human adenovirus type 12. | | 1967 | 6061217 |
| general characteristics of the cells transformed in vitro by human adenovirus type 12. | | 1967 | 6061218 |
| synthesis of virus deoxyribonucleic acid during abortive infection of simian cells by human adenoviruses. | rapp, fred (baylor university college of medicine, houston, tex.), lawrence a. feldman, and manley mandel. synthesis of virus deoxyribonucleic acid during abortive infection of simian cells by human adenoviruses. j. bacteriol. 92:931-936. 1966.-inoculation of green monkey kidney cells (gmk) with adenovirus types 2 or 12, under conditions where neither infectious virus was synthesized, resulted in an increase in the uptake of h(3)-thymidine into deoxyribonucleic acid (dna). extraction of the dna ... | 1966 | 5927211 |
| on the nature of transplantation immunity in the adenovirus tumor system. | the existence of a virus-induced, virus-specific transplantation, antigen in adenovirus 12-induced cba mouse tumors was demonstrated. the antigen is virus-specific, but not related to structural virion or t antigens. it is a weak antigen, and required immunization with whole, infectious adenovirus 12 to produce considerable immunity. comparable immunity could not be achieved with homologous cellular or subcellular materials, but some indication of enhancement was produced with low tumor dose. im ... | 1967 | 6025323 |
| incubation period for tumor induction by adenovirus type 12. | | 1967 | 6081228 |
| transformation of hamster cells in vitro by adenovirus. type 12. | | 1967 | 6081696 |
| low molecular weight rnas with homologies to cellular dna at sites of adenovirus dna insertion in hamster or mouse cells. | the adenovirus type 2 (ad2)-transformed hamster cell line he5 contains one or very few integrated copies of ad2 dna. at the site of insertion of ad2 dna, the cellular dna sequence has been completely preserved and has homologies to small unpolyadenylated, cytoplasmic rnas of 300 nucleotides in length and to minority populations of smaller rnas present in he5 cells and in normal hamster cells. the 300-nucleotide rna is present on average in approximately 20 copies per cell. this rna, and shorter ... | 1984 | 6084594 |
| the effect of calcium on cell lines derived from adenovirus type 12-induced hamster tumors. | | 1965 | 5890603 |
| routine establishment of serial lines of hamster embryo cells transformed by adenovirus type 12. | | 1967 | 6067291 |
| adenovirus type 12 transformation involves loss of beta-adrenergic receptors and isoproterenol responsiveness. | the responsiveness of a growth-regulated rat 3y1 cell line and five clones of 3y1 cells transformed by the highly oncogenic human adenovirus type 12 to the catecholamine hormone (-)-isoproterenol was studied. the untransformed cells contained beta-adrenergic receptors characterized by specific binding of the beta-adrenergic receptor antagonist (-)-[3h]dihydroalprenolol, a 9- to 12-fold increase in cyclic amp production in intact cells after incubation with 10 microm (-)-isoproterenol, and signif ... | 1982 | 6152838 |
| difference in growth factor requirements of rat 3y1 cells among growth in mass culture, clonal growth in low density culture, and stimulation to enter s phase in resting culture. | a semiserum-free medium was developed for monolayer culture of rat 3y1 fibroblastic cells. the main components of the developed medium added to dulbecco's modified eagle's medium (dmem) were insulin, transferrin, epidermal growth factor, poly-d-lysine, bovine albumin, oleic acid, and bovine alpha-globulin. in this medium, 3y1 cells grew in mass culture at much the same rate as in dmem supplemented with 10% fetal bovine serum (fbs), and colonies, albeit of smaller sizes, did form. virally transfo ... | 1983 | 6190731 |
| adenovirus type 12 specific cell surface antigen in transformed cells is a product of the e1b early region. | six syngeneic rat cell lines transformed with isolated or cloned left end fragments of adenovirus type 12 (ad12) dna were used in a study of the ad12-specific cell surface antigen. cells transformed with ecori-c, sali-c, and hindiii-g fragments of ad12 dna fragment-transformed express the e1a and a part of or a complete e1b early regions. two acci-h dna fragment-transformed cell lines contain and express only the e1a region. all these cells contain nuclear ad12 antigen. cytotoxic antibodies rais ... | 1983 | 6197809 |
| the left part of the viral genome is sufficient for interferon induction by adenovirus type 12. | adenovirus type 12 is a potent interferon inducer on chick embryo cells. incomplete particles induce similar levels of interferon as complete virions, even if they contain only the left 20 per cent of the genome. empty capsids lacking dna are not able to induce interferon, suggesting that part of the viral genome is required to trigger the cells to produce interferon. | 1984 | 6199003 |
| analysis of proteins, helper dependence, and seroepidemiology of a new human parvovirus. | a new type of defective parvovirus, tentatively designated as adeno-associated virus type 5 (aav-5), is characterized as far as its proteins, its helper dependence, and its seroepidemiology are concerned. the protein analysis of aav-5 in polyacrylamide gels demonstrated the presence of three structural polypeptides, corresponding to vp 1, vp 2, and vp 3 of other aav types. the preparation of monoclonal antibodies against aav-5 permitted the analysis of viral structural antigen expression by usin ... | 1984 | 6200995 |
| adenovirus type 12 early region 1 proteins: a study of their subcellular localization and protein-protein interactions. | subcellular fractionation of rat and human cells transformed by the adenovirus type 12 (ad-12) ecori-c dna fragment showed that the 41000 mol. wt. (41k) e1a and 52k e1b proteins were present in the nucleus and cytoplasm at approximately equal concentrations. the 18k e1b protein was associated with the nuclear, mitochondrial, lysosomal and membrane fractions. the 41k e1a protein was also associated with various cytoskeletal structures (probably microtubules and 10 nm filaments) in ad-12-transform ... | 1984 | 6239909 |
| methylation of integrated adenovirus type 12 dna sequences in transformed cells is inversely correlated with viral gene expression. | the adenovirus type 12 (ad12) dna sequences integrated into the dna of four lines of ad12-transformed hamster cells are extensively methylated. methylation in mammalian cell dna is believed to occur predominantly at 5'-c-g-3' sequences. the majority, although not all, of the 5'-c-c-g-g-3' sequences present in integrated ad12 dna are methylated. ad12 dna isolated from purified virions, on the other hand, is not methylated to any significant extent. the segments of the integrated viral dna compris ... | 1980 | 6244548 |
| alteration of growth rate and nutritional requirement of hamster brain cells transformed by adenovirus type 12 during their long-term cultivation. | | 1967 | 6064506 |
| viruses as tools for studies on the molecular biology of mammalian cells. | 1. multiple copies of intact adenovirus type 12 (ad12) dna are integrated into the dna of ad12-transformed hamster and ad12-induced rat brain tumor cells. free viral dna is not present in the ad12-transformed lines investigated. 2. only few sites of integration are found in al2-transformed hamster and ad12-induced rat brain tumor cells. integration may have occurred into repetitive cellular sequences at selective sites. these sites may be different in different cell lines, however, none of these ... | 1980 | 6248089 |
| neoplastic transformation of chimpanzee cells induced by adenovirus type 12--simian virus 40 hybrid virus. | the adenovirus 12--simian virus 40 hybrid virus produced neoplastic transformation of chimpanzee skin fibroblasts in vitro. the transformed fibroblasts showed morphological alteration and became permanent lines. the transformed cells contained both adenovirus 12 and simian virus 40 large tumor antigens and were virus producers. however at passage 9, one line (wes) was found to be a nonproducer, producing neither infectious virus nor virus-specific antigen detectable by the complement fixation te ... | 1981 | 6264437 |
| an unusual symmetric recombinant between adenovirus type 12 dna and human cell dna. | on purification of human adenovirus type 12 (ad12) by equilibrium sedimentation in cscl density gradients, two bands of particles, ad12-3 and ad12-3a, are observed. the particles from band ad12-3a contain a recombinant of human host cell dna and of ad12 dna. the human cell dna sequences contain repetitive dna recurring 200 to 500 times in cellular dna. ad12 dna and the recombinant genomes exhibit the same or similar lengths. this finding suggests that a constant amount of dna is packaged into co ... | 1981 | 6265937 |
| mapping of rnas transcribed from adenovirus type 12 early and va rna regions. | | 1981 | 6270880 |
| complement-dependent cytotoxicity in rats bearing human adenovirus type 12-induced primary retinoblastoma-like tumor in the eye. | using an animal model of retinoblastoma in inbred rats and cultured human adenovirus type 12-induced retinoblastoma-like tumor cells (rao 188), complement-dependent cytotoxicity was determined by measuring release of 3h-uridine labelled rna. sera from rats in which tumors did not grow after adenovirus type 12 inoculation had higher cytotoxicity against rao 188 cells than sera from rats bearing primary adenovirus type 12-induced retinoblastoma-like tumor. these results showed that the rat which c ... | 1981 | 6271500 |
| cloned fragments of human adenovirus type-12 dna. | the following restriction endonuclease fragments of human adenovirus type 12 (ad12) dna have been cloned in plasmid or bacteriophage lambda vectors using standard protocols: the ecori-a*, -b, -d, -e, and -f fragments, the bamhi-b, -c, -d, -f, -g, -h, and -i fragments, the hindiii-f and -i fragments, and the psti-a, -d, -f, -g, and -h fragments. the ecori-a* fragment comprises the right terminal 5 kb of ad12 dna including the terminal 143 bp. | 1981 | 6271646 |
| nucleotide sequence of the transforming early region e1b of adenovirus type 12 dna: structure and gene organization, and comparison with those of adenovirus type 5 dna. | the nucleotide sequence of the entire transforming early region of e1b of the highly oncogenic adenovirus type 12 (ad12) dna has been determined. the total sequence (3860 base pairs) encompasses the entire transforming early region e1 of ad12 dna. from the sequence for the e1b region of ad12, and the transcription map of the e1b region (1, 2, 3, and this paper) the structure and gene organization of the early region e1b of ad12 dna were analyzed and compared with those of the e1b region in the n ... | 1981 | 6275367 |
| two tumor antigens and their polypeptides in adenovirus type 12-infected and transformed cells. | a tumor (t) antigen, designated t antigen g, was visualized as fine fluorescent granules in nuclei of adenovirus type 12 (ad12)-infected cells by immunofluorescence with sera from rats bearing hy cell tumors (h sera). hy cells are rat cells incompletely transformed by the acc i-h endonuclease fragment (0-4.7 map units) of ad12 dna. the antigen is different from the usually described t antigen, designated t antigen f, which is visualized as fluorescent flecks or filaments in both nucleus and cyto ... | 1980 | 6246533 |
| undifferentiated neuroectodermal tumors induced by human adenovirus type 31 in syrian hamsters. | human adenovirus type 31 was inoculated intraperitoneally into 18 and intracranially into 98 syrian hamsters within 24 hours of birth. intraperitoneal tumors developed multicentrically in 94.4%, 93 days on an average after virus inoculation, while brain tumors developed in 6.1%, 154 days. intraperitoneal tumors primarily developed in the subserosal tissues and protruded into the peritoneal cavity. some of the five enlarged brain tumors seemed to have extended from the paraventricular regions to ... | 1984 | 6098127 |
| immunological and virological investigations in down's syndrome. | lymphocyte responsiveness to phytohaemagglutinin and viral antigens was studied in children with down's syndrome and in controls. mitogen-responsiveness in the patients was significantly reduced as compared to the control values. using the lymphocyte transformation test, trisomic patients showed more than a twofold increase in sensitivity to herpes simplex virus as compared to controls. the same test did not show any essential difference between the two groups when adeno- and influenza viruses w ... | 1982 | 6281021 |
| shifts in the extent and patterns of dna methylation upon explanation and subcultivation of adenovirus type 12-induced hamster tumor cells. | | 1982 | 6281967 |
| integrated adenovirus type 12 dna in the transformed hamster cell line t637: sequence arrangements at the termini of viral dna and mode of amplification. | approximately 20 to 22 copies of adenovirus type 12 (ad12) dna per cell were integrated into the genome of the cell line t637. only a few of these copies seemed to remain intact and colinear with virion dna. all other persisting viral genomes exhibited deletions or inversions or both in the right-hand part of ad12 dna. spontaneously arising morphological revertants of t637 cells has lost viral dna. in most of the revertant cell lines only the intact or a part of the intact viral genome was prese ... | 1982 | 6283150 |
| cloning of dna fragments from the left end of the adenovirus type 12 genome: transformation by cloned early region 1. | the human adenovirus serotype 12 (ad-12) ecori-c dna fragment (0 to 16.5 map units) was cloned in the plasmid vector pat153. this cloned ad-12 ecori-c dna fragment was subcloned generating recombinant plasmids which contained the ad-12 sali-c fragment (0 to 10.3 map units), the ad-12 hindiii-g fragment (0 to 6 x 8 map units) and the ad-12 acci-h fragment (0 to 4 x 7 map units). thus, we constructed recombinant plasmids which contain ad-12 dna sequences which represent all or part of the virus tr ... | 1982 | 6286853 |
| expression of adenovirus type 12 e1a gene in monkey cells, using a simian virus 40 vector. | simian virus 40 (sv40) recombinants carrying the adenovirus type 12 e1a gene were constructed. the sv40 expression vector was constructed by removing most of the vp1 gene and an internal part of the intervening sequence for late 16s rna and by joining the 5' and 3' splice sites into a small segment. the adenovirus type 12 e1a gene with or without its own promoter was inserted downstream from the sv40 late promoter and the splicing junctions. the recombinant dna was propagated and packaged in mon ... | 1983 | 6296446 |
| hyperproduction of adenovirus type 12 e1b gene product in monkey cells, using a simian virus 40 vector. | simian virus 40 recombinant dnas carrying the adenovirus type 12 e1b gene were constructed, propagated, and packaged in monkey cells. monkey cells infected with the resulting virus stocks hyperproduced the e1b gene products in more than 80% of the cells as revealed by immunofluorescence. the products were distributed in both the nuclei and the cytoplasm, and a condensed form of fleck structure was observed in the cytoplasm. polyacrylamide gel electrophoresis of the cell extracts and their immuno ... | 1983 | 6296447 |