| characterization of arcanobacterium abortisuis by phenotypic properties and by sequencing the 16s-23s rdna intergenic spacer region. | the present study was designed to characterize phenotypically and genotypically nine arcanobacterium abortisuis strains collected from specimen of pigs in a period of nine years. all nine a. abortisuis strains and a. abortisuis reference strain dsm 19515 displayed a synergistic hemolytic reaction with staphylococcus aureus β-hemolysin, rhodococcus equi, and arcanobacterium haemolyticum indicator strains and showed the typical biochemical properties of this species. the species identity could be ... | 2010 | 20926209 |
| necrotising fasciitis in a bull due to infection with arcanobacterium haemolyticum. | a 2-year-old hereford bull was lame for one week before becoming recumbent. clinical and pathological findings: the scrotum and ventral perineal region were cold and blackened caudally. the semimembranosus and semitendinosus muscles were firm on palpation. the bull was subject to euthanasia, and necropsy revealed that the skin and s/c tissues of the caudal half of the scrotum were grey and necrotic. the caudal and distal aspects of the semimembranosus and semitendinosus muscles were grey and nec ... | 2010 | 20927177 |
| phospholipase d promotes arcanobacterium haemolyticum adhesion via lipid raft remodeling and host cell death following bacterial invasion. | arcanobacterium haemolyticum is an emerging bacterial pathogen, causing pharyngitis and more invasive infections. this organism expresses an unusual phospholipase d (pld), which we propose promotes bacterial pathogenesis through its action on host cell membranes. the pld gene is found on a genomic region of reduced %g + c, suggesting recent horizontal acquisition. | 2010 | 20973961 |
| antibiotic susceptibility of bacteria isolated from respiratory tract of pigs in poland between 2004 and 2008. | antibiotic susceptibility of bacteria isolated from nasal swabs and lungs of pigs, to 16 commonly used antibiotics, was determined by disc diffusion test. beta-lactams showed the best activity against streptococcus suis (s. suis) (> 99% of susceptible strains). the lowest sensitivity of s. suis was evidenced to: tylosin, tetracycline and neomycin (50%, 40% and 25%, respectively). isolates of escherichia coli (e. coli) demonstrated the highest susceptibility to cephalosporin (85% strains), gentam ... | 2010 | 21077428 |
| normal bacterial flora from vaginas of criollo limonero cows. | in order to describe the normal bacterial flora in vaginas of criollo limonero cows, 51 healthy multiparous cows, at least 90-day postpartum, were selected. duplicated swabs (n = 102) were taken from the vaginal fornix of cows to perform aerobic and anaerobic cultures as well as conventional biochemical tests. out of 102 swabs, bacterial growth was obtained in 55 (53.9%) while the remaining 47 (46.1%) did not exhibited any bacterial growth. of the 55 bacterial growths, 23 (41.8%) were aerobic wh ... | 2010 | 21082249 |
| diagnosis of intramammary infection in samples yielding negative results or minor pathogens in conventional bacterial culturing. | up to half of quarter milk samples submitted for mastitis diagnosis are culture-negative results or lead to identification of coagulase-negative staphylococci or corynebacterium bovis in conventional culturing, the so-called minor pathogens. the interpretation and usefulness of these results in terms of udder and animal health management is limited, even though the amount of resources spent is relatively high. this work aimed to test two methods of analysis of milk samples with the goal of incre ... | 2010 | 21134309 |
| arcanobacterium bernardiae bacteremia in a patient with deep soft tissue infection. | | 2010 | 21166597 |
| determination of ceftiofur derivatives in serum, endometrial tissue, and lochia in puerperal dairy cows after subcutaneous administration of ceftiofur crystalline free acid. | puerperal uterine infections are often associated with decreased reproductive performance in dairy cows. routine treatment protocols include the systemic administration of antibiotics. antibiotic drugs, however, should be administered daily over at least 5 d. the objective of this study was to determine concentrations of ceftiofur derivatives in serum, endometrial tissue, and lochia after subcutaneous administration of ceftiofur crystalline free acid in 6 clinically healthy puerperal dairy cows ... | 2011 | 21183038 |
| aborted lesions of a pig associated with arcanobacterium abortisuis and the immunohistochemical features. | a microorganism isolated from an aborted sow's placenta has been proposed as a novel species, arcanobacterium abortisuis. the lesions in the placenta were suppurative and necrotic placentitis, whereas lesions of three fetuses were suppurative bronchopneumonia. gram and grocott-positive organisms in these lesions were immunolabeled with anti- arcanobacterium abortisuis antibody. this is the first case report, which showed the association of arcanobacterium abortisuis with the aborted lesions of a ... | 2011 | 21224529 |
| identification of arcanobacterium haemolyticum isolated from postcastrational complications of a horse. | an arcanobacterium haemolyticum strain isolated from a postcastrational lesion of a horse was identified phenotypically and genotypically. the latter was performed by sequencing the 16s-23s rdna intergenic spacer region (isr), by amplification of the gene encoding a. haemolyticum phospholipase d, by amplification of a. haemolyticum specific parts of isr-23s rdna and by amplification of the newly described camp factor family protein encoding gene of a. haemolyticum. this indicates (as described p ... | 2010 | 21253917 |
| complete genome sequence of arcanobacterium haemolyticum type strain (11018). | arcanobacterium haemolyticum (ex maclean et al. 1946) collins et al. 1983 is the type species of the genus arcanobacterium, which belongs to the family actinomycetaceae. the strain is of interest because it is an obligate parasite of the pharynx of humans and farm animal; occasionally, it causes pharyngeal or skin lesions. it is a gram-positive, nonmotile and non-sporulating bacterium. the strain described in this study was isolated from infections amongst american soldiers of certain islands of ... | 2010 | 21304742 |
| identification of corynebacterium pseudotuberculosis from sheep by pcr-restriction analysis using the rna polymerase ß-subunit gene (rpob). | caseous lymphadenitis, caused by corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including argentina and brazil. a polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) method for the identification of this microorganism was designed based on the hypervariable region of the polymorphic rna polymerase ß-subunit gene (rpob). all available corynebacteriumrpob sequences were analyzed by computer-assisted restriction analysis. the rpob p ... | 2011 | 21419467 |
| molecular identification and further characterization of arcanobacterium pyogenes isolated from bovine mastitis and from various other origins. | the present study was designed to identify phenotypically and genotypically 61 arcanobacterium pyogenes isolated from bovine mastitis and from various other origins. the a. pyogenes isolates showed the typical cultural and biochemical properties of this species and displayed camp-like synergistic hemolytic activities with various indicator strains. the species identity could be confirmed genotypically by amplification and sequencing of the superoxide dismutase a encoding gene soda of reference s ... | 2011 | 21426970 |
| [arcanobacterium haemolyticum brain abscess mimicking a brain tumor]. | | 2011 | 21440388 |
| cutaneous diphtheria in the urban poor population of vancouver, british columbia: a 10-year review. | between 1998 - 2007, records from 33 patients with cutaneous diphtheria from vancouver's inner-city were reviewed. cases were associated with injection drug use and poverty. co-infections with staphylococcus aureus, streptococcus pyogenes and arcanobacterium haemolyticum occurred. corynebacterium diphtheriae is endemic in vancouver's urban core, with mlst sequence type 76 strains predominating. | 2011 | 21525220 |
| acute phase proteins in milk in naturally acquired bovine mastitis caused by different pathogens. | the concentrations of haptoglobin (hp) and serum amyloid a (saa) and the activity of n-acetyl-β-d-glucosaminidase (nagase) in milk from 234 cows with spontaneous mastitis caused by different pathogens were measured to assess whether they corresponded with the clinical signs of mastitis and whether there were any differences between pathogens. ninety-eight of the cows had clinical mastitis and 136 had subclinical mastitis. there were statistically significant positive correlations between the con ... | 2011 | 21558129 |
| infective endocarditis caused by arcanobacterium haemolyticum: a case report. | abstract: arcanobacterium haemolyticum is an organism that commonly causes pharyngitis and wound infections. it does not usually cause systemic invasive disease. the organism presents a difficult diagnostic problem because the clinical microbiology laboratory has a propensity to view them as diphtheroid organisms of the corynebacterium species, thus contaminants or normal flora. we describe a case of a 21-year-old female who had endocarditis with cerebral emboli due to arcanobacterium haemolytic ... | 2011 | 21569379 |
| chorioamnionitis due to arcanobacterium haemolyticum. | clinical chorioamnionitis can result either from the ascending organisms from vagina after rupture of membranes or via the blood stream. this report describes a case of chorioamnionitis caused by arcanobacterium haemolyticum, an unusual organism associated with this infection. a 19-year-old primigravida at 32 weeks of gestation presented with the complaints of fever, pain in the abdomen, and discharge per vaginum for the past 2 days. watery, odorless, colorless, and discharge passing intermitten ... | 2011 | 21572617 |
| microbiological identification and analysis of swine tonsils collected from carcasses at slaughter. | the primary objective of this 7-month study was to determine the prevalence of porcine pathogens of the tonsil of the soft palate of swine at slaughter. additional objectives were to determine if sampling the carcasses of normal or abnormal hogs provided different microbiological profiles and if the slaughter plant provides a feasible sampling frame and environment for detecting and monitoring important pathogens in tonsils that have health implications for both swine and humans. a total of 395 ... | 2011 | 21731180 |
| sequential arcanobacterium haemolyticum and escherichia coli spinal infections after local excision of a rectal polyp. | | 2011 | 21679653 |
| development of a pulsed-field gel electrophoresis (pfge) method for molecular typing of clinical isolates of arcanobacterium haemolyticum. | we developed a two-block pfge method to study molecular variation among clinical isolates of arcanobacterium haemolyticum, an often overlooked human pathogen. three main macrorestriction profiles were defined among 15 isolates. pfge was an objective method for characterizing a. haemolyticum and may be useful in molecular epidemiological studies of this organism. | 2011 | 21689692 |
| Arcanobacterium canis sp. nov., isolated from an otitis externa of a dog and emended description of the genus Arcanobacterium Collins et al. 1982 emend. Yassin et al. 2011. | A polyphasic taxonomic study was performed on previously unidentified Arcanobacterium-like Gram-positive bacteria isolated from otitis externa of a dog.Comparative 16S rRNA gene sequencing showed that the bacteria belonged to genus Arcanobacterium and were most closely related to Arcanobacterium haemolyticum (97.2%), Arcanobacterium hippocoleae (96.5%) and Arcanobacterium phocae (96.4%). The presence of the major menaquinone MK-9 H4 supported the affiliation of this strain to genus Arcanobacteri ... | 2011 | 22081713 |
| rapid identification of bovine mastitis pathogens by high-resolution melt analysis of 16s rdna sequences. | accurate identification of mastitis pathogens is often compromised when using conventional culture-based methods. here, we report a novel, rapid assay tested for speciation of bacterial mastitis pathogens using high-resolution melt analysis (hrma) of 16s rdna sequences. real-time pcr amplification of 16s rrna gene fragment, spanning the variable region v5 and v6 was performed with a resulting amplicon of 290bp. first, a library was generated of melt curves of 9 common pathogens that are implicat ... | 2011 | 21944716 |
| Arcanolysin is a cholesterol-dependent cytolysin of the human pathogen Arcanobacterium haemolyticum. | Arcanobacterium haemolyticum is an emerging human pathogen that causes pharyngitis, wound infections, and a variety of occasional invasive diseases. Since its initial discovery in 1946, this Gram positive organism has been known to have hemolytic activity, yet no hemolysin has been previously reported. A. haemolyticum also displays variable hemolytic activity on laboratory blood agar that is dependent upon which species the blood is derived. | 2011 | 22029628 |
| [arcanobacterium bernardiae]. | | 2011 | 21879152 |
| Reproductive tract defense and disease in postpartum dairy cows. | This paper briefly reviews recent data and concepts on the development and mitigation of infection and inflammation in the reproductive tract of dairy cows during the first 2 mo after calving. The incidence of metritis is typically between 10 and 20%, of clinical endometritis or purulent vaginal discharge (PVD) approximately 15%, and of subclinical or cytological endometritis a further 15%. Worse postpartum negative energy balance is associated with more severe or prolonged uterine inflammation. ... | 2011 | 21890187 |
| hessian fly-associated bacteria: transmission, essentiality, and composition. | plant-feeding insects have been recently found to use microbes to manipulate host plant physiology and morphology. gall midges are one of the largest groups of insects that manipulate host plants extensively. hessian fly (hf, mayetiola destructor) is an important pest of wheat and a model system for studying gall midges. to examine the role of bacteria in parasitism, a systematic analysis of bacteria associated with hf was performed for the first time. diverse bacteria were found in different de ... | 2011 | 21858016 |
| distribution of arcanobacterium pluranimalium in animals examined in veterinary laboratories in the united kingdom. | arcanobacterium pluranimalium was first reported in 2001 for 2 isolates, from a harbor porpoise (phocoena phocoena) and a fallow deer (dama dama). the same organism was subsequently reported from ovine material. a review of material submitted to veterinary laboratories in the united kingdom from a range of domestic and wild animals during the following years revealed that a. pluranimalium had been recovered from ovine specimens on 33 occasions. twenty ovine isolates came from abortion material, ... | 2011 | 21908355 |
| the effect of recurrent episodes of clinical mastitis caused by gram-positive and gram-negative bacteria and other organisms on mortality and culling in holstein dairy cows. | the objective of this study was to estimate the effects of recurrent episodes of different types of clinical mastitis (cm) caused by gram-positive (streptococcus spp., staphylococcus aureus, staphylococcus spp.) and gram-negative (escherichia coli, klebsiella, citrobacter, enterobacter, pseudomonas) bacteria, and other organisms (arcanobacterium pyogenes, mycoplasma, corynebacterium bovis, yeast, miscellaneous) on the probability of mortality and culling in holstein dairy cows. data from 30,233 ... | 2011 | 21943738 |
| detection and characterization of antibiotic-resistance genes in arcanobacterium pyogenes strains from abscesses of forest musk deer. | arcanobacterium pyogenes is commonly isolated from ruminant animals as an opportunistic pathogen that co-infects with other bacteria, normally causing surface or internal abscesses. twenty-eight strains of a. pyogenes isolated from forest musk deer suppurative samples were identified by their 16s rrna gene sequences, and confirmed by amplification of the pyolysin-encoding gene (plo) in all isolates. the mics of 14 commonly used antibiotics were determined by an agar dilution method. class 1 and ... | 2011 | 21852523 |
| Phenotypic and molecular characterization of Arcanobacterium haemolyticum isolated from clinical samples. | The aim of this study was to assess the phenotypic and genotypic diversity of 56 Arcanobacterium haemolyticum isolates isolated from 51 patients attending primary health care centres and emergency units in the health area of Santander (Cantabria, northern Spain). Phenotypic characterization was based on morphological, biochemical, and antigenic tests. Species identification was confirmed by amplification and sequencing of the 16S rDNA gene. Antimicrobial susceptibility testing was determined by ... | 2012 | 22001620 |
| Association between virulence factors of Escherichia coli, Fusobacterium necrophorum, and Arcanobacterium pyogenes and uterine diseases of dairy cows. | The objective of this study was to evaluate the relationship between bacterial species-specific virulence factors (VFs) present in the uterus at 3 different stages of lactation (1-3, 8-10, and 34-36 days in milk (DIM)) and the incidence of metritis and clinical endometritis in dairy cows. The following VF genes were investigated: plo (pyolysin), cbpA (collagen-binding protein), and fimA (fimbriae expression) which are Arcanobacterium pyogenes specific; fimH (a type 1 pilus component), Escherichi ... | 2011 | 22186615 |
| Identification of Trueperella (Arcanobacterium) bernardiae by MALDI-TOF mass spectrometry analysis and by species specific PCR. | In the present study an Arcanobacterium (Trueperella) bernardiae strain previously isolated from a bacteremia of a patient with a deep soft tissue infection could successfully be identified by MALDI-TOF MS and genotypically by sequencing the RNA polymerase encoding gene rpoB and the 16S-23S rDNA intergenic spacer region. The latter also allowed the design of species specific oligonucleotide primers which could be used for PCR-mediated identification of A. (T.) bernardiae. | 2011 | 22096130 |
| Rapid identification of the animal pathogens Streptococcus uberis and Arcanobacterium pyogenes by fluorescence in situ hybridization (FISH). | Fluorescence in situ hybridization (FISH) has been reported to be an easy and rapid identification method for many human pathogens, but applications for common veterinary pathogens are lacking. Gene probes for FISH of the animal pathogens Streptococcus uberis and Arcanobacterium pyogenes were designed to provide probes for a specific identification of these bacteria from cultures. Specific FISH probes for these species have so far not been published. Both probes recognized all isolates of the ta ... | 2011 | 22033042 |
| [A frequently overlooked bacteria in clinical microbiology laboratories: arcanobacterium haemolyticum]. | Arcanobacterium haemolyticum, previously known as Corynebacterium haemolyticum, is a facultative anaerobic, gram-positive bacillus with negative catalase and positive CAMP inhibition test results. It may be the causative agent of about 0.5-3% of acute bacterial pharyngitis especially in children and young adults. Since growth of A.haemolyticum is usually inhibited by flora members and since it slowly develops hemolysis in sheep blood agar and its colony morphology resembles beta-hemolytic strept ... | 2011 | 21935787 |
| Antimicrobial susceptibility of Arcanobacterium pyogenes isolated from the lungs of white-tailed deer (Odocoileus virginianus) with pneumonia. | In vitro susceptibilities of 29 strains of Arcanobacterium pyogenes isolated from lung lesions of white-tailed deer (Odocoileus virginianus) with pneumonia were determined using the broth microdilution method to ascertain efficacious treatment options for pneumonic white-tailed deer. All 29 A. pyogenes strains tested were susceptible to ceftiofur, spectinomycin, tiamulin, and trimethoprim-sulfamethoxazole but were resistant to both danofloxacin and sulfadimethoxine. Likewise, all 29 isolates wer ... | 2011 | 21908365 |
| evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) for species identification of bacteria of genera arcanobacterium and trueperella. | in the present study matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) was evaluated for species identification of 98 bacteria previously classified phenotypically and genotypically to genera arcanobacterium and trueperella. species identification was carried out by comparing the main spectra of each strain with the main spectra of reference strains of both genera and 3740 database entries included in the maldi biotyper 2.0 software package (bruker dalto ... | 2011 | 22270885 |