| resistance to dieldrin in lucilia cuprina wied., the australian sheep blowfly. | | 1958 | 13526706 |
| survival of type 1 and type 3 polio vaccine virus in blowflies. (phaenicia sericata) at 40 degree c. | | 1965 | 14301631 |
| composition of the surface hydrocarbons from the vitelline membranes of dipteran embryos. | hydrocarbons were the major lipid class extracted by hexane from the vitelline membrane surface of dechorionated eggs of the house fly, musca domestica, the new world screwworm, cochliomyia hominivorax, the secondary screwworm, cochliomyia macellaria, the green bottle fly, phaenicia sericata, the sheep blow fly, lucilia cuprina and the mexican fruit fly, anastrepha ludens. the length of time the embryos must be exposed to hexane with or without a small amount of alcohol in order to attain permea ... | 2003 | 14529755 |
| methods used for the killing and preservation of blowfly larvae, and their effect on post-mortem larval length. | a record of the length of the largest larvae collected from a corpse can be used to estimate the age of the oldest larvae present and, therefore, give an estimate of minimum time since death. consequently, factors that affect post-mortem larval length will impact on any estimate of pmi based on it. methods used to kill and preserve larvae are known to affect post-mortem length. this study looks at the effects of different preservatives, and variations in the protocol used for killing larvae by i ... | 2003 | 14642719 |
| diptera of sanitary importance associated with composting of biosolids in argentina. | odorous compounds produced at the biosolids composting plant in bariloche (nw patagonia) attract a variety of insects, mainly belonging to the order diptera. in order to characterize these flies, collected specimens were taxonomically identified, their community characteristics were described and their sanitary and synanthropic importance and autochthonous or introduced character were determined. | 2003 | 14666301 |
| progress towards the development of a transgenic strain of the australian sheep blowfly (lucilia cuprina) suitable for a male-only sterile release program. | the australian sheep blowfly lucilia cuprina is the most important pest species involved in cutaneous myiasis (flystrike) of sheep in australia and new zealand. in new zealand l. cuprina is primarily controlled through the application of insecticides. however, there is an increased interest in biological methods of control of this species. we have proposed to develop a genetically modified strain of l. cuprina that would be ideal for a male-only sterile release program. to that end we have devel ... | 2004 | 14871615 |
| report of a case of human nasal myiasis by the green-bottle fly, phaenicia sericata, in san bernardino county, california. | | 1952 | 14943920 |
| the survival of poliomyelitis and coxsackie viruses following their ingestion by flies. | poliomyelitis virus and coxsackie (or c) virus were quantitatively fed to blowflies, phormia regina and phaenicia sericata, and to houseflies, musca domestica. naturally infectious human stools were the source of virus. poliomyelitis virus can be almost quantitatively recovered from flies and from their excreta collected over a period of several days following the feeding. c virus can also be recovered but in lesser yields. no conclusive evidence for virus multiplication in these laboratory-bred ... | 1952 | 14955579 |
| effects of temperature and photoperiod on the termination of larval diapause in lucilia sericata (diptera: calliphoridae). | larvae of the blow fly, lucilia sericata (meigen), enter diapause in the third instar after cessation of feeding. the effects of temperature and photoperiod on the termination of diapause were examined. the diapause terminated spontaneously under the diapause-inducing condition of 20 degrees c and ld 12:12, although pupariation was not synchronous. diapause development proceeded under a low temperature of 7.5 degrees c. transfer to long-day conditions of ld 16:8 or to a high temperature of 25 de ... | 2004 | 14993832 |
| effects of population density and cadmium toxicity on growth and survival of blowflies. | the blowfly lucilia sericata has been reared on artificial diets containing from 0 to 200 mg cd kg(-1) at population densities ranging from 8 larvae per 4-g diet to 8 larvae per 32-g diet. peak larval weight, development period, pupal weight, adult-emergence weight, and fecundity were measured. larvae of blowflies respond to food deprivation by a reduction in larval weight and subsequent pupal and adult size. contaminating the food supply with cadmium produced similar additive effects, reduced t ... | 1993 | 15091835 |
| myiasis in kuwait: nosocomial infections caused by lucilia sericata and megaselia scalaris. | myiasis, the invasion of live human tissue by larvae of diptera, is reported in the nasopharynx and a leg wound in two patients who were hospitalized for more than 72 hours in mubarak al-kabeer teaching hospital in kuwait city, kuwait. on the fourth and fifth days after a 10-year-old kuwaiti boy was admitted to the hospital intensive care unit in a bloodied and comatose state following a traffic accident, 'worms' that came out of his nostrils were fixed, cleared, and identified as second and thi ... | 2004 | 15100451 |
| determination of poultry feed-through insecticide activity in an in vivo anticoccidial assay. | the ability of an in vivo anticoccidial assay to identify potential feed-through insecticides was demonstrated using first-instar lucilia sericata on droppings from chicks fed medicated diets. cyromazine, a commercially available feed-through insect growth regulator, ivermectin, diflubenzuron, fipronil, permethrin, and 2 experimental compounds were effective in varying degrees in killing l. sericata larvae. eleven coccidiostats were effective against the protozoan parasites (eimeria spp.) at com ... | 2004 | 15165074 |
| initial study of arthropod succession on pig carrion in a central european urban habitat. | we conducted a carrion succession study within a restricted urban backyard in the city of vienna, austria (16 degrees 22'e, 48 degrees 12'n) from may to november 2001 to analyze sequence and composition of the local carrion visiting fauna. two medium sized clothed domestic pig carcasses (sus scrofa linnaeus), were used as surrogate human models. in total, 42 arthropod species from the families calliphoridae, sarcophagidae, sepsidae, piophilidae, muscidae, fanniidae, sphaeroceridae, phoridae, dro ... | 2004 | 15185958 |
| blowflies calliphora vicina and lucilia sericata as passive vectors of mycobacterium avium subsp. avium, m. a. paratuberculosis and m. a. hominissuis. | mycobacterium avium subsp. paratuberculosis (actinomycetales: mycobacteriaceae) isolates of identical restriction fragment length polymorphism (rflp) type b-c1 were isolated from: intestinal mucosa of two cows showing clinical signs of paratuberculosis, a specimen of the blowfly calliphora vicina robineau-desvoidy (diptera: calliphoridae) captured while perched on these cattle intestines in a waste container at the site of the slaughter, and the blowflies c. vicina and lucilia caesar linnaeus ca ... | 2004 | 15189236 |
| factors related to the attraction of flies at a biosolids composting facility (bariloche, argentina). | the composting process is used to treat biosolids from the wastewater treatment plant of bariloche (nw patagonia, argentina). since 1998, an odourless, innocuous and stable organic amendment has been produced at the biosolids composting plant of bariloche. however, volatile compounds produced during this process, attract different vectors, mainly insects belonging to the order diptera, particularly in summer. to evaluate factors associated with the attraction of diptera to composting windrows, v ... | 2004 | 15207571 |
| composition of chemical attractants affects trap catches of the australian sheep blowfly, lucilia cuprina, and other blowflies. | numbers of lucilia cuprina (australian sheep blowfly), chrysomya spp., and calliphora spp. blowflies caught on sticky traps baited with various synthetic attractants or a standard liver/sodium sulfide attractant in western queensland were recorded. numbers of each genus collected were influenced by the composition of the chemical attractants. attractant mixtures based on 2-mercaptoethanol, indole, butanoic/pentanoic acid, and a sodium sulfide solution gave 5- to 20-fold higher l. cuprina catches ... | 2004 | 15260228 |
| toxicity of the entomopathogenic fungus, metarhizium anisopliae (deuteromycotina: hyphomycetes) to adult females of the blowfly lucilia sericata (diptera: calliphoridae). | the fungus metarhizium anisopliae (metchnikoff) (deuteromycotina: hyphomycetes) is a natural component of soil flora world-wide and is a causal agent of the green muscardine diseases of insects. the use of this pathogen as a potential biocontrol agent against adult females of the blowfly, lucilia sericata (meigen) (diptera: calliphoridae) was considered. the blowfly l sericata is an economically important agent of cutaneous myiasis of sheep, particularly in northern europe. exposure of flies to ... | 2004 | 15260293 |
| the reactions of lucilia sericata (mg.) to various substances placed on sheep. | | 1950 | 15401183 |
| [the study of the major sarcosaphagous flies in hohhot]. | in order to determine the major species of sarcosaphagous flies and their regular activity on carcass in hohhot district. | 2004 | 15495802 |
| detection and partial characterisation of two antibacterial factors from the excretions/secretions of the medicinal maggot lucilia sericata and their activity against methicillin-resistant staphylococcus aureus (mrsa). | maggot therapy is a simple and highly successful method for cleansing infected and necrotic wounds. the use of maggots has become increasingly important in the treatment of non-healing wounds, particularly those infected with the multidrug-resistant pathogen, methicillin-resistant staphylococcus aureus (mrsa). the increasing challenge concerning the treatment of mrsa infections and the recent finding of vancomycin-resistant strains of mrsa have elicited the search for novel antibacterial compoun ... | 2004 | 15555536 |
| estimation of postmortem interval in real cases based on experimentally obtained entomological evidence. | using the entomological evidence obtained in several forensic cases analyzed in our laboratory for comparison, we evaluated the results of an experimental study carried out in a semiurban setting to determine the structure of the sarcosaprophagous fauna from a mediterranean region of se spain. in all, 18 orders of arthropods were collected. the summarized experimental results refer to the most important taxa for estimating the postmortem interval. thus, the seasonal character of certain diptera ... | 2005 | 15734111 |
| evaluation of efficacy of entomopathogenic nematodes against larvae of lucilia sericata (meigen, 1826) (diptera: calliphoridae). | the blowfly lucilia sericata (meigen, 1826) (diptera: calliphoridae) is the primary agent of cutaneous myiasis of sheep in northern europe, southern africa, australia and new zealand. as the application of chemicals has several disadvantages, alternative control measures of traumatic myiasis of livestock must be developed. in this study, the use of entomopathogenic nematodes (epns) as potential biocontrol agents against second instar larvae of lucilia sericata was considered. the following nemat ... | 2005 | 15782660 |
| multiple mutations and gene duplications conferring organophosphorus insecticide resistance have been selected at the rop-1 locus of the sheep blowfly, lucilia cuprina. | sequences of the esterase gene alpha e7 were compared across 41 isogenic (iv) strains of the sheep blowfly, lucilia cuprina, and one strain of the sibling species, l. sericata. the 1.2-kb region sequenced includes sites of two insecticide resistance mutations. gly137asp confers resistance to organophosphorus insecticides (ops), particularly preferring diethyl ops such as diazinon, while trp251leu prefers dimethyl ops, and particularly malathion, with the additional presence of carboxylester moie ... | 2005 | 15785849 |
| rearing larvae of lucilia sericata for chronic ulcer treatment--an improved method. | larval therapy is a biological 'dressing' for chronic ulcers which is used for debridement, to initiate healing and to reduce bacteria and unpleasant odour. one condition for being able to employ larval therapy is access to disinfected larvae of good quality. we therefore started rearing lucilia sericata larvae in 1998. rearing necrophagous larvae in a hospital setting makes it essential to control odour. the improvement of the method includes the use of a controlled, humid, warm environment wit ... | 2005 | 15823905 |
| insect fauna visiting carrion in southwest virginia. | successional patterns of insect fauna on pig carcasses were studied in southwest virginia. the objective was to identify and qualitatively assess the major taxa of forensic importance in this region. studies were conducted in spring and summer 2001 and 2002, and fall 2002. over 50 taxa were collected and identified. phormia regina was the dominant fly species in the spring (>90%) and co-dominant with phaenicia coeruleiviridis in the summer. phaenicia sericata, lucilia illustris, and calliphora s ... | 2005 | 15837010 |
| winter survival of larvae and pupae of the blowfly, lucilia sericata (diptera: calliphoridae). | the mortality of larvae and pupae of the blowfly lucilia sericata (meigen) were examined during winter and spring, for two or three years. in soil cores in the field, 70-95% of the larvae died overwinter. larvae congregated in the top 10 cm of the soil core and did not move extensively throughout the column during the winter. larvae and pupae at greater depth were less likely to pupariate and emerge successfully than larvae closer to the surface. under semi-natural conditions, where pupae were p ... | 2005 | 15960872 |
| gene expression of heat-shock proteins (hsp23, hsp70 and hsp90) during and after larval diapause in the blow fly lucilia sericata. | genes encoding heat-shock protein 23 (hsp23), hsp70 and hsp90 were cloned from lucilia sericata to examine whether their expression is related to the regulation of its larval diapause. the level of all three hsps mrnas was consistently low irrespective of diapause status. these results indicate that expression of hsp23, hsp70 and hsp90 is not regulated in response to diapause, in contrast to the flesh fly sarcophaga crassipalpis that shows upregulation of hsp23 and hsp70 and downregulation of hs ... | 2005 | 15993128 |
| structural conservation of the salivary gland-specific slalom gene in the blowfly lucilia sericata. | glycosylation and sulfation are two of the essential post-translational modifications of proteins. the slalom gene encodes a 3'-phosphoadenosine 5'-phosphosulfate transporter, a conserved protein found in organisms as diverse as plants and humans and required for sulfation of proteins. in drosophila, slalom is exclusively expressed in salivary glands, which is unexpected, taken into account the general function for sulfation of proteins. in this paper, we present a detailed description of the sl ... | 2005 | 16003524 |
| maggots and wound healing: an investigation of the effects of secretions from lucilia sericata larvae upon the migration of human dermal fibroblasts over a fibronectin-coated surface. | lucilia sericata larvae, or greenbottle fly maggots, placed within chronic wounds have been observed to remove necrotic tissue and infection. they are also believed to actively promote granulation tissue formation. interactions between fibroblasts and the surrounding extracellular matrix play a crucial role in tissue formation, influencing fibroblast proliferation, migration, and tissue remodeling. for example, the strength of cell adhesion to surfaces coated with extracellular matrix influences ... | 2005 | 16008732 |
| the effects of artificially-induced fly-strike on food intake and liveweight gain in sheep. | a single, artificially-induced fly-strike with lucilia sericata larvae was associated with a rapid (decline in food intake in sheep, with a consequent reduction in liveweight. loss of weight ranged from 0.5 to 5.5 kg over four to six days and recovery to pre-infestation liveweight took three to 36 days. pair-fed, uninfested partners of these sheep also showed a reduction in liveweight, whereas uninfested sheep in some experiments fed ad libitum showed either little change or a gain in liveweight ... | 1987 | 16031371 |
| identification and characterization of proteins from bacillus thuringiensis with high toxic activity against the sheep blowfly, lucilia cuprina. | current control of the sheep blowfly (lucilia cuprina) relies on chemical insecticides, however, with the development of resistance and increasing concerns about human health and environmental residues, alternative strategies to control this economically important pest are required. in this study, we have identified several isolates of bacillus thuringiensis (bt), collected from various australian soil samples, that produce crystals containing 130 and 28 kda proteins. these isolates were highly ... | 2005 | 16098986 |
| sound radiation around a flying fly. | many insects produce sounds during flight. these acoustic emissions result from the oscillation of the wings in air. to date, most studies have measured the frequency characteristics of flight sounds, leaving other acoustic characteristics--and their possible biological functions--unexplored. here, using close-range acoustic recording, we describe both the directional radiation pattern and the detailed frequency composition of the sound produced by a tethered flying (lucilia sericata). the flapp ... | 2005 | 16119372 |
| antibacterial properties of larval secretions of the blowfly, lucilia sericata. | the antibacterial properties of secretions aseptically collected from larvae of the greenbottle fly lucilia sericata (meigen) (diptera: calliphoridae) were examined. these investigations revealed the presence of small (<1 kda) antibacterial factor(s) within the larval secretions, active against a range of bacteria. these include the gram-positive staphylococcus aureus, both methicillin-resistant staphylococcus aureus (mrsa) and methicillin-sensitive staphylococcus aureus (mssa), streptococcus py ... | 2005 | 16134984 |
| characterization of a new bacillus thuringiensis endotoxin, cry47aa, from strains that are toxic to the australian sheep blowfly, lucilia cuprina. | sixteen isolates of bacillus thuringiensis, derived from various soil samples collected in australia, are highly toxic to larvae of the sheep blowfly (lucilia cuprina). the toxin gene from one of the strains (caa890) was cloned by genome walking, and sequencing of the cloned fragments revealed a new cry gene, encoding a protein of 1134 amino acid residues, with a theoretical molecular mass of 139,209da. based on the amino acid sequence comparison with known cry delta-endotoxins, the gene was des ... | 2005 | 16168574 |
| control of sheep blowfly strike using fly-traps. | the use of fly-traps for the control of sheep blowfly strike was examined on 12 commercial sheep farms in south west england in 2003. two flocks acted as controls, with no prophylactic blowfly strike control. four flocks of lambs and three flocks of ewes were protected only by blowfly traps, seven of the flocks of ewes and six flocks of lambs were protected by blowfly traps but also had proprietary insecticides applied to them at some stage. there was no difference in the abundance of l. sericat ... | 2006 | 16188387 |
| from patterns to processes and back: analysing density-dependent responses to an abiotic stressor by statistical and mechanistic modelling. | our knowledge about population-level effects of abiotic stressors is limited, largely due to lack of appropriate time-series data. to analyse interactions between an abiotic stressor and density-dependent processes, we used experimental time-series data for stage-structured populations (the blowfly lucilia sericata) exposed to the toxicant cadmium through 20 generations. resource limitation results in competition both in the larval and the adult stages. the toxicant has only negative effects at ... | 2005 | 16191626 |
| wingless signaling in a large insect, the blowfly lucilia sericata: a beautiful example of evolutionary developmental biology. | blowflies are the primary facultative agent in causing myiasis of domestic sheep in the whole world and, at the same time, it is an important tool for forensic medicine. surprisingly, and in contrast to its importance, almost no data regarding the embryology and molecular markers are known for this insect. in this report, we present a detailed description of the blowfly lucilia sericata embryogenesis and of imaginal disc development. the embryogenesis of lucilia strongly resembles that of drosop ... | 2006 | 16258966 |
| [effect of morphine on the development of lucilia sericata larvae]. | | 2005 | 16300020 |
| comparison of octopamine-like immunoreactivity in the brains of the fruit fly and blow fly. | a serum raised against conjugated octopamine reveals structurally comparable systems of perikarya and arborizations in protocerebral neuropils of two species of diptera, drosophila melanogaster and phaenicia sericata; the latter is used extensively for electrophysiological studies of the optic lobes and their central projections. clusters of cell bodies in the brain as well as midline perikarya provide octopamine-like immunoreactive processes to the optic lobes, circumscribed regions of the prot ... | 2006 | 16320256 |
| growth rates of the blowfly, lucilia sericata, on different body tissues. | estimates of post-mortem interval based on forensic analysis of the age of blowfly larvae on a corpse, are generated from standardised larval development curves. these are derived from studies of the growth of larvae reared, usually, on liver and at a range of temperatures. however, it is possible that the species or organ on which the larvae feed, might significantly alter the rate of growth. the present study therefore compared the development of the blowfly lucilia sericata (meigen) (diptera: ... | 2006 | 16410165 |
| flystrike in new zealand: an overview based on a 16-year study, following the introduction and dispersal of the australian sheep blowfly, lucilia cuprina wiedemann (diptera: calliphoridae). | a summary and analysis have been carried out on data from over 6000 instances of flystrike in sheep in nz, over a 16-year period, using a self-referral system where farmers submitted larvae and related information. the study covered a period of establishment and subsequent countrywide spread of the exotic blowfly, lucilia cuprina. comparisons are drawn between flystrike as it was perceived by surveys carried out before the 1940s, and the current situation with l. cuprina as an added major impedi ... | 2006 | 16464534 |
| [identification of sarcosaphagous flies by analyses the sequence of 16s rdna]. | to solve the difficulties of identification of sarcosaphagous flies such as lucilia sericata (meigen) and lucilia cuprina (wiedemann) which could not be identified by analyzing the 278bp and 635 bp regions of the gene encoding for cytochrome oxidase subunit i and ii (co i and co ii) in mtdna. | 2006 | 16524183 |
| factors affecting the transmission of salmonella by flies: natural resistance to colonization and bacterial interference. | groups of immobilized gnotobiotic houseflies, musca domestica l., were mono-contaminated with graded doses of salmonella typhimurium, bicontaminated with s. typhimurium and proteus mirabilis, and multicontaminated with s. typhimurium and a mouse fecal flora. of 45 flies fed an average of 22 salmonella cells alone, 26 showed bacterial multiplication, up to 1.4 x 10(7) in one case. there was a fairly consistent increase in percentage of salmonella excreters with increasing input, so that all flies ... | 1970 | 16557919 |
| cytogenetic studies of calliphora vicina and lucilia sericata (diptera: calliphoridae) from northwestern egypt. | the blow fly, calliphora vicina (robineau-desvoidy, 1830) and the green bottle fly, lucilia sericata (meigen, 1826) were collected from the north coast of egypt. the karyotype of both flies was characterized by cytotaxonomical methods, including chromosome number, shape, relative length, arm ratio, centromeric index and designation. to quantify relative chromosome length, the percent of total haploid autosomal complement length (%tacl) for each autosome was calculated. the deploid number of chro ... | 2006 | 16605097 |
| from the bible to biosurgery: lucilia sericata--plastic surgeon's assistant in the 21st century. | | 2006 | 16641761 |
| promotion of human dermal fibroblast migration, matrix remodelling and modification of fibroblast morphology within a novel 3d model by lucilia sericata larval secretions. | lucilia sericata larvae, or green bottle fly maggots are applied to chronic wounds to aid healing. previously, our laboratory has characterized the enzymatic activities present within maggot excretions/secretions (es). since then, we have related these to the degradation of extracellular matrix components, alteration of human, dermal fibroblast adhesion to surfaces and the stimulation of fibroblast migration within a two-dimensional in vitro assay. in this study, we developed a novel three-dimen ... | 2006 | 16675968 |
| diversity of calliphoridae (diptera) in brazil's tinguá biological reserve. | the calliphoridae are flies of great ecological, medical and sanitary importance because they are decomposers of organic matter, mechanical vectors of pathogenic agents, and causers of myiasis. this paper attempts to ascertain the diversity of calliphoridae in the tinguá biological reserve and correlate meteorological data (temperature, relative humidity of the air, and precipitation) with the occurrence of these flies. the study was conducted at a site in the tinguá biological reserve, located ... | 2006 | 16680310 |
| amplification of dna from preserved specimens shows blowflies were preadapted for the rapid evolution of insecticide resistance. | mutations of esterase 3 confer two forms of organophosphate resistance on contemporary australasian lucilia cuprina. one form, called diazinon resistance, is slightly more effective against commonly used insecticides and is now more prevalent than the other form, called malathion resistance. we report here that the single amino acid replacement associated with diazinon resistance and two replacements associated with malathion resistance also occur in esterase 3 in the sibling species lucilia ser ... | 2006 | 16723400 |
| on an enzyme from blow-fly larvae [lucilia sericata] which digests collagen in alkaline solution. | | 1931 | 16744710 |
| the nitrogen metabolism of an insect (lucilia sericata mg.): uric acid, allantoin and uricase. | | 1938 | 16746701 |
| the nitrogen metabolism of an insect (lucilia sericata mg.): ammonia and other metabolites. | | 1938 | 16746702 |
| maggot therapy: the science and implication for cam part i-history and bacterial resistance. | it is now a universally acknowledged fact that maggot therapy can be used successfully to treat chronic, long-standing, infected wounds, which have previously failed to respond to conventional treatment. such wounds are typically characterized by the presence of necrotic tissue, underlying infection and poor healing. maggot therapy employs the use of freshly emerged, sterile larvae of the common green-bottle fly, phaenicia (lucilia) sericata, and is a form of artificially induced myiasis in a co ... | 2006 | 16786052 |
| isolation and insecticidal/anthelmintic activity of xanthonol, a novel bis-xanthone, from a non-sporulating fungal species. | xanthonol, a novel dimeric xanthone, was isolated from a fermentation broth of a non-sporulating fungal species using sephadex lh20 followed by hplc and the structure elucidated by spectral analysis. xanthonol exhibited insecticidal and anthelmintic activities against larvae of lucilia sericata, aedes aegypti, and haemonchus contortus with ld90 of 33, 8, and 50 microg/ml, respectively. | 2006 | 16883778 |
| karyotypes, constitutive heterochromatin, and genomic dna values in the blowfly genera chrysomya, lucilia, and protophormia (diptera: calliphoridae). | the karyotypes and c-banding patterns of chrysomya species c. marginalis, c. phaonis, c. pinguis, c. saffranea, c. megacephala (new guinean strain), lucilia sericata, and protophormia terraenovae are described. all species are amphogenic and have similar chromosome complements (2n = 12), including an xy-xx sex-chromosome pair varying in size and morphology between species. additionally, the c-banding pattern of the monogenic species chrysomya albiceps is presented. the dna contents of these and ... | 2006 | 16936838 |
| identification of necrophagous fly species using issr and scar markers. | necrophagous fly is the most common insect evidence collected during a death investigation. we analyzed the dna polymorphism among the five forensically important fly species, namely, lucilia sericata, aldrichina grahami, chrysomya megacephala, parasarcophaga crassipalpis and musca domestica using inter simple sequence repeat (issr) method. nine issr primers selected from 18 primers could amplify 105 clear and stable bands, of which 95 bands were polymorphic. some primers produced completely dif ... | 2007 | 16949234 |
| maggot therapy: the science and implication for cam part ii-maggots combat infection. | maggot therapy employs the use of freshly emerged, sterile larvae of the common green-bottle fly, phaenicia (lucilia) sericata, and is a form of artificially induced myiasis in a controlled clinical situation. maggot therapy has the following three core beneficial effects on a wound: debridement, disinfection and enhanced healing. in part ii of this review article, we discuss clinical infections and the evidence supporting the potent antibacterial action of maggot secretions. enhancement of woun ... | 2006 | 16951714 |
| components of developmental plasticity in a michigan population of lucilia sericata (diptera: calliphoridae). | forensic entomologists rely on laboratory growth data to estimate the time of blow fly colonization on human remains. several data sets exist for the development of the common blow fly lucilia sericata (meigen) (diptera: calliphoridae), and although they generally describe similar rates of preadult development, all vary. such differences could be explained by genetic variation, environmental (rearing) variation, or both. in the study presented here, flies from a single population were reared und ... | 2006 | 17017243 |
| visual system of calliphorid flies: motion- and orientation-sensitive visual interneurons supplying dorsal optic glomeruli. | intracellular recordings accompanied by dye fills were made from neurons associated with optic glomeruli in the lateral protocerebrum of the brain of the blowfly phaenicia sericata. the present account describes the morphology of these cells and their electrophysiological responses to oriented bar motion. the most dorsal glomeruli are each supplied by retinotopic efferent neurons that have restricted dendritic fields in the lobula and lobula plate of the optic lobes. each of these lobula complex ... | 2007 | 17099892 |
| diverse speed response properties of motion sensitive neurons in the fly's optic lobe. | speed and acceleration are fundamental components of visual motion that animals can use to interpret the world. behavioral studies have established that insects discriminate speed largely independently of contrast and spatial frequency, and physiological recordings suggest that a subset of premotor descending neurons is in this sense speed-selective. neural substrates and mechanisms of speed selectivity in insects, however, are unknown. using blow flies phaenicia sericata, intracellular recordin ... | 2007 | 17106704 |
| greenbottle (lucilia sericata) larval secretions delivered from a prototype hydrogel wound dressing accelerate the closure of model wounds. | the resurgence of larval biotherapy as a debridement tool in wound management has been accompanied by several clinical reports highlighting concomitant tissue regeneration. studies employing in vitro cell motility assays have found that purified excretory/secretory (es) products from greenbottle larvae (blowfly, lucilia sericata) are motogenic for human dermal fibroblasts when used as a supplement in culture media. the objective of the present study was to determine whether es delivered using a ... | 2006 | 17137319 |
| in vitro antibacterial activity of lucilia sericata maggot secretions. | maggots of the green blowfly, lucilia sericata, are used as an alternative to surgical intervention and long-term antiseptic therapy for the treatment of chronic wounds. the secretions of maggots are known to have antibacterial properties. to quantify the bactericidal effect of secretions from larvae of l. sericata, an in vitro test model based on the modified european quantitative suspension test (en 1040) was developed, in which a co-culture of maggots and bacteria (micrococcus luteus,escheric ... | 2007 | 17167275 |
| blowfly succession from possum (trichosurus vulpecula) carrion in a sheep-farming zone. | the significance of brushtail possum, trichosurus vulpecula kerr (diprotodontia: phalangeridae) carcasses to the succession and production of diptera species and its relevance to fly strike management in tasmania, australia was examined. calliphora stygia (fabricius), lucilia sericata (meigen) and calliphora vicina robineau-desvoidy (diptera: calliphoridae) were found to be the most abundant and lucilia cuprina (wiedemann) (diptera: calliphoridae) always the least abundant (< 1%) of the putative ... | 2006 | 17199756 |
| lifetime reproductive output of calliphora vicina and lucilia sericata in outdoor caged and field populations; flight vs. egg production? | females of the blowflies calliphora vicina (robineau-desvoidy) and lucilia sericata (meigen) (diptera: calliphoridae) maintained in sheltered outdoor cages and supplied with excess food, oviposited later than would have been expected from the temperature-sum. the survival rates of the caged flies was high and the isolation of flies from predation, extreme temperatures and food shortages is likely to have contributed to this. despite good survival rates, subsequent egg production over the greater ... | 2006 | 17199757 |
| diazinon resistance, fluctuating asymmetry and fitness in the australian sheep blowfly, lucilia cuprina. | genetic evidence suggests that the evolution of resistance to the insecticide diazinon in lucilia cuprina initially produced an increase in asymmetry. at that time resistant flies were presumed to be at a selective disadvantage in the absence of diazinon. subsequent evolution in natural populations selected modifiers to ameliorate these effects. the fitness and fluctuating asymmetry levels of resistant flies are currently similar to those of susceptibles. previous genetic analyses have shown the ... | 1988 | 17246476 |
| flight activity of the blowflies, calliphora vomitoria and lucilia sericata, in the dark. | many species of insects are able to fly at night or in very low light intensities. the question of whether calliphorid blowflies are also able to do this to locate a corpse and oviposit nocturnally is of considerable forensic importance. however, to date studies of this behaviour have been contradictory. here, the activity and number of calliphora vomitoria l. and lucilia sericata (meigen) (diptera: calliphoridae) caught on sticky-traps were examined in a slow-speed wind tunnel, at different int ... | 2007 | 17267152 |
| [relationship between co ii gene of mtdna of lucilia sericata and latitude interval]. | to deduce the region that the geographical species of lucilia sericata come from and determine the scene of crime (soc) based on the gene analysis of mtdna co ii. | 2006 | 17285856 |
| maggot excretions/secretions inhibit multiple neutrophil pro-inflammatory responses. | there is renewed interest in the use of maggots (lucilia sericata) to aid in healing of chronic wounds. in such wounds neutrophils precipitate tissue damage rather than contribute to healing. as the molecules responsible for the beneficial actions of maggots are contained in their excretions/secretions (es), we assessed the effects of es on functional activities of human neutrophils. es dose-dependently inhibited elastase release and h(2)o(2) production by fmlp-activated neutrophils; maximal inh ... | 2007 | 17350304 |
| antibacterial properties of whole body extracts and haemolymph of lucilia sericata maggots. | to partially characterise maggot-secreted antibacterial substances and determine their range of activity against different bacteria. | 2007 | 17385589 |
| formation of boldenone and boldenone-analogues by maggots of lucilia sericata. | current evidence suggests that neo formation of the anabolic steroid boldenone (androsta-1,4-diene-17-ol-3-one) occurs in calves' faecal material, making it difficult to distinguish between illegally administered boldenone and its potential endogenous presence. this strengthens the urgent need to elucidate the pathway leading to boldenone formation. in our laboratory, the invertebrate neomysis integer (crustacea, mysidacea) was used since 2004 as an alternative model for the partial replacement ... | 2007 | 17386708 |
| use of sterile maggots in an unusual trauma injury. | sterile larvae of the common green bottle, lucilia sericata, have been shown to be a highly cost-effective alternative to conventional treatments for the debridement and cleansing of all types of chronic wounds. (thomas, 2006). one or two treatment cycles each lasting approximately 3 days are often sufficient to achieve complete debridement of such wounds, provided sufficient numbers of maggots are applied on each application. maggots are less commonly used in traumatic injuries, although in the ... | 2007 | 17505399 |
| cuticular hydrocarbon composition in pupal exuviae for taxonomic differentiation of six necrophagous flies. | gas chromatography-mass spectrometry was used to analyze the cuticular hydrocarbons extracted from the pupal exuviae of six necrophagous flies: aldrichina grahami (aldrich), chrysomya megacephala (f.), lucilia sericata (meigen), achoetandrus rufifacies (macquart), boettcherisca peregrina (robineau-desvoidy), and parasarcophaga crassipalpis (macquart). a discriminant model including the variables of peak 1 (tricosane), peak 7 (9-,11-,13-methyl-pentacosane), peak 21 (11,12-;9,13-dimethyl-hexacosan ... | 2007 | 17547230 |
| antibacterial substances of low molecular weight isolated from the blowfly, lucilia sericata. | low molecular weight compounds were isolated by high-performance liquid chromatography from the maggot or haemolymph extracts of lucilia sericata (meigen) (diptera: calliphoridae). using gas chromatography-mass spectrometry analysis, three compounds were obtained: p-hydroxybenzoic acid (molecular weight 138 da), p-hydroxyphenylacetic acid (molecular weight 152 da) and octahydro-dipyrrolo[1,2-a;1',2'-d] pyrazine-5,10-dione (molecular weight 194 da), also known as the cyclic dimer of proline (or p ... | 2007 | 17550431 |
| larval therapy from antiquity to the present day: mechanisms of action, clinical applications and future potential. | when modern medicine fails, it is often useful to draw ideas from ancient treatments. the therapeutic use of fly larvae to debride necrotic tissue, also known as larval therapy, maggot debridement therapy or biosurgery, dates back to the beginnings of civilisation. despite repeatedly falling out of favour largely because of patient intolerance to the treatment, the practice of larval therapy is increasing around the world because of its efficacy, safety and simplicity. clinical indications for l ... | 2007 | 17551073 |
| the nocturnal oviposition behaviour of blowflies (diptera: calliphoridae) in central europe and its forensic implications. | numerous factors may cause delayed colonisation of a corpse by blowflies, leading to a discrepancy between the entomologically determined post-mortem interval (pmi) and the time of death. blowflies, for example, are considered to be inactive at night, however, published observations are contradictory. in the present study, several field experiments and one type of indoor experiment were conducted in summer of 2004 and 2005 in order to investigate the nocturnal ovipositional behaviour of blowflie ... | 2008 | 17587519 |
| cerebral myiasis associated with angiosarcoma of the scalp: case report. | primary human cerebral myiasis is an exceedingly rare condition and is almost never encountered by physicians in developed countries. the case report summarizes a case of extensive cerebral myiasis in a periurban community in the united states. | 2007 | 17621006 |
| the therapeutic behavior of lucilia sericata meig. larvae in osteomyelitis wounds. | | 1934 | 17821749 |
| fly abundance and climate as determinants of sheep blowfly strike incidence in southwest england. | the relationships between abundance of the blowfly lucilia sericata (meigen) (diptera: calliphoridae), climate, animal management procedures and the incidence of cutaneous myiasis (blowfly strike) in sheep were examined in three sheep pasture systems in southwest england during the summers of 2002 and 2003. in each year, flies were collected using liver-baited sticky targets, daily weather and routine husbandry practices were noted and the age-class of each animal infested and body position of e ... | 2007 | 17897363 |
| [human myiasis in bahía blanca, argentina. period 2000 / 2005]. | myiasis is the infestation of live human and vertebrate animals with dipterous larvae which, at least for a short period, feed on the host's dead or living tissue, liquid body-substance, or ingested food. the objective of this study was to identify the flies producing myiasis in bahía blanca city, argentina, from 01/03/2000 to 31/05/2005. seventeen clinical cases were studied. the larvae obtained from lesions were forwarded from laboratories and from public and private hospitals. part of the lar ... | 2007 | 17934629 |
| maggot excretions/secretions are differentially effective against biofilms of staphylococcus aureus and pseudomonas aeruginosa. | lucilia sericata maggots are successfully used for treating chronic wounds. as the healing process in these wounds is complicated by bacteria, particularly when residing in biofilms that protect them from antibiotics and the immune system, we assessed the effects of maggot excretions/secretions (es) on staphylococcus aureus and pseudomonas aeruginosa biofilms, the clinically most relevant species. | 2008 | 17965032 |
| clinical and microbiological efficacy of mdt in the treatment of diabetic foot ulcers. | to assess the clinical and microbiological efficacy of maggot debridement therapy (mdt) in the management of diabetic foot ulcers unresponsive to conventional treatment and surgical intervention. | 2007 | 17987750 |
| the application of mitochondrial dna cytochrome oxidase ii gene for the identification of forensically important blowflies in western china. | blowflies found on human corpses are important for the estimation of the postmortem interval and other questions of forensic relevance. some of these species are difficult to differentiate morphologically, and therefore a molecular method was elaborated for species identification. here, we describe a molecular method for rapid identification of these insects. specific insect dna fragments were amplified using the polymerase chain reaction, followed by direct dna sequencing of the amplification p ... | 2007 | 18043017 |
| species composition and seasonal succession of saprophagous calliphorids in a rural area of córdoba: argentina. | during 2004, four experiments were carried out, one each season, in order to determine the species composition and seasonal dynamics of calliphoridae in a rural area of córdoba (argentina). two pigs (sus scrofa l.), weighing approximately 8 kg each, were used in each experiment. they were killed with a blow to the head and immediately placed in a variant of the schoenly et al. (1991) trap. one pig was exposed in the shade and the other one under direct sunlight. the insect fauna was collected da ... | 2007 | 18064353 |
| codeine accumulation and elimination in larvae, pupae, and imago of the blowfly lucilia sericata and effects on its development. | the aim of this study was to evaluate the reliability of insect larvae as samples for toxicological investigations. for this purpose, larvae of lucilia sericata were reared on samples of minced pig liver treated with different concentrations of codeine: therapeutic, toxic, and potentially lethal doses. codeine was detected in all tested larvae, confirming the reliability of these specimens for qualitative toxicology analysis. furthermore, concentrations measured in larvae were correlated with le ... | 2008 | 18064481 |
| use of lucilia species for forensic investigations in southern europe. | the aim of this study was to highlight the importance of evaluating entomological evidence in forensic investigations on a regional scale. to evaluate climatic, geographical and environmental influences on the selection of carrion-breeding fauna in northern italy and consequently on inferred forensic data (post-mortem intervals and post-mortem transfer), we present details of six indoor-outdoor cases. results show that the most abundant species was lucilia sericata, together with other fly speci ... | 2008 | 18079080 |
| in vitro induction of 60-kda and 70-kda heat shock proteins by endosulphan and monocrotophos in sheep blowfly lucilia cuprina. | the effect of two most commonly used and highly toxic organic pesticides, endosulphan (organochlorine) and monocrotophos (organophosphate), was studied in a blowfly, lucilia cuprina, to test whether these pesticides induce the stress response and, if so, whether the intensity of the response, in terms of induction of heat shock proteins (hsps), hsp60 and hsp70 in particular, is pesticide concentration dependent. the in vitro exposure of larval and adult tissues to varying concentrations of these ... | 2008 | 18080081 |
| [maggots of lucilia sericata in treatment of intractable wounds]. | although beneficial effects of wound infestation with maggots had been known for many centuries, it was not until dr zacharias recognized medical importance of maggots during the american civil war. he intentionally introduced maggots into the wound for its debridement. baer successfully used maggots in treatment of osteomyelitis in 4 children in the 1930'. after many successes in the 1930' maggots therapy had become limited to intractable wounds after introducing sulphonamides and mass-producti ... | 2007 | 18175560 |
| a review of the use of maggots in wound therapy. | chronic wounds have been defined as those that do not follow the orderly manner of healing, and thus do not achieve closure. such wounds are notoriously difficult to treat and the number of treatments around attest to the fact that management of chronic wounds is not possible with one agent and definitely never achieves the most satisfactory results in a period short enough to satisfy the patient. maggots had been used sparingly initially and are gradually finding their way into a more acceptabl ... | 2008 | 18216520 |
| shape and function of the bicoid morphogen gradient in dipteran species with different sized embryos. | the bicoid morphogen evolved approximately 150 mya from a hox3 duplication and is only found in higher dipterans. a major difference between dipteran species, however, is the size of the embryo, which varies up to 5-fold. although the expression of developmental factors scale with egg length, it remains unknown how this scaling is achieved. to test whether scaling is accounted for by the properties of bicoid, we expressed egfp fused to the coding region of bicoid from three dipteran species in t ... | 2008 | 18328473 |
| mitochondrial genomes of the sheep blowfly, lucilia sericata, and the secondary blowfly, chrysomya megacephala. | this paper presents complete mitochondrial genomes for the sheep blowfly, lucilia sericata (meigen), and the secondary blowfly, chrysomya megacephala (fabricius). both l. sericata and c. megacephala had standard dipteran-type mitochondrial genome architectures and lengths of 15 945 bp and 15 831 bp, respectively. additionally, c. megacephala possessed a trna duplication either side of the d-loop, as previously reported in another chrysomya species, c. putoria; this duplication appears to be syna ... | 2008 | 18380659 |
| the antibacterial activity against mrsa strains and other bacteria of a <500da fraction from maggot excretions/secretions of lucilia sericata (diptera: calliphoridae). | the application of lucilia sericata larvae to chronic, infected wounds results in the rapid elimination of infecting microorganisms, including mrsa. previously, we demonstrated in vitro antibacterial activity of native excretions/secretions (nes) from l. sericata and partially purified two low mass antibacterial compounds with masses of 0.5-10kda and <500da. the present study reports the antibacterial effects of the <500da fraction (es<500) on the growth and morphology of a range of bacteria, in ... | 2008 | 18400544 |
| selective antimicrobial activity of maggots against pathogenic bacteria. | maggot therapy, also known as biosurgery, is an ancient method for the healing of chronic infected wounds. although clinicians have reported on the beneficial activities of the lucilia sericata larvae that have been used for healing chronic wounds, the selectivity of this therapy against the different pathogenic micro-organisms that are found in chronic wounds has never been analysed. in the present study, we have investigated the in vitro activities of larval excreta/secreta both against select ... | 2008 | 18436596 |
| [identification of necrophagous fly species from 12 different cities and regions in china using inter-simple sequence repeat melocular markers]. | to identify necrophagous fly species from different regions in china using inter-simple sequence repeat (issr) melocular markers and analyze their genetic difference and relationship. | 2008 | 18495581 |
| generalized additive models and lucilia sericata growth: assessing confidence intervals and error rates in forensic entomology. | forensic entomologists use blow fly development to estimate a postmortem interval. although accurate, fly age estimates can be imprecise for older developmental stages and no standard means of assigning confidence intervals exists. presented here is a method for modeling growth of the forensically important blow fly lucilia sericata, using generalized additive models (gams). eighteen gams were created to predict the extent of juvenile fly development, encompassing developmental stage, length, we ... | 2008 | 18503527 |
| influxed insects as vectors for campylobacter jejuni and campylobacter coli in danish broiler houses. | the vector potential of flies (diptera: brachycera) for spread of campylobacter jejuni and campylobacter coli on 5 danish broiler farms was evaluated in a longitudinal field study from april to november 2004. first, the prevalence of c. jejuni- and c. coli-positive flies was determined in 2,816 flies captured from farm surroundings. each individual fly was macerated, preenriched in bolton broth for 24 h at 42 degrees c, streaked onto modified campylobater blood-free selective agar and incubated ... | 2008 | 18577626 |
| the fine structure and deposition of the larval cuticle of the sheep blowfly (lucilia cuprina). | the cuticle of lucilia is composed of an untanned endocuticle and a complex epicuticle of four layers, superficial layer, outer epicuticle, cuticulin and dense layer. the outer epicuticle and attached epicuticular filaments are resistant to acid hydrolysis. during deposition of the cuticle of each larval instar, the cuticulin and dense layers are formed first, followed by the outer epicuticle, which appears to be laid down by secretions from the epidermis passing through the cuticulin via epicut ... | 1970 | 18631527 |
| maggot debridement therapy: a case study. | a 59-year-old woman arrived at vancouver general hospital after a motor vehicle accident in which she sustained massive degloving injuries to both lower extremities. due to her faith (she is a jehovah's witness), traditional interventions such as surgical debridement and revision were not possible and consent to receive blood products was not provided. woc nursing was consulted at the request of the family to utilize maggot debridement therapy (mdt) to treat her necrotic lower extremities. initi ... | 2008 | 18635997 |
| maggots do not survive in pyoderma gangrenosum. | application of maggots is well established in wound therapy. we report upon 2 patients with leg ulcers due to pyoderma gangrenosum who received immunosuppressive therapy and in whom a reduced survival of maggots (lucilia sericata sp.) led to ineffectiveness of this therapy. | 2008 | 18663307 |
| [preliminary evaluation of maggot (diptera: calliphoridae) therapy as a potential treatment for leishmaniasis ulcers]. | maggot debridement therapy has been widely used for treating a variety of scarred-over soft-tissue wounds. published accounts record several illnesses in which treatment with larval therapy has promoted injury healing in conjunction with infection by bacterial pathogens resistant to conventional antibiotics. | 2008 | 18719732 |
| [biology of the phaenicia sericata fly in different substrata]. | dipterans of the species phaenicia sericata were captured and placed in cages in order to evaluate their biological cycle and to define the best substratum for posture and development of the larvae in controlled temperature and humidity (27 degrees c e 80% ur). four decomposing substrata were evaluated: bovine, chicken and fish meat as well as bovine liver. no statistical differences were observed in the three first substrata and no posture occurred in the liver. the same substrata were used for ... | 2008 | 18823572 |
| the influence of maggot excretions on pao1 biofilm formation on different biomaterials. | biofilm formation in wounds and on biomaterials is increasingly recognized as a problem. it therefore is important to focus on new strategies for eradicating severe biofilm-associated infections. the beneficial effects of maggots (lucilia sericata) in wounds have been known for centuries. we hypothesized sterile maggot excretions and secretions (es) could prevent, inhibit, and break down biofilms of pseudomonas aeruginosa (pao1) on different biomaterials. therefore, we investigated biofilm forma ... | 2009 | 18923881 |