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rearing temperature enhances hepatic glucokinase but not glucose-6-phosphatase activities in european sea bass (dicentrarchus labrax) and gilthead sea bream (sparus aurata) juveniles fed with the same level of glucose.the aim of this work was to elucidate if the previous results observed in hepatic glucokinase (gk) and glucose-6-phosphatase (g6pase) activities in european sea bass and gilthead sea bream are due to temperature per se or to differences in feed intake at different water temperatures. for that purpose triplicate groups of fish (30 g initial body weight) were kept at 18 degrees c or 25 degrees c during two weeks and fed a fixed daily ration of a glucose-free or 20% glucose diet. at the end of the ...200818508397
nodavirus increases the expression of mx and inflammatory cytokines in fish brain.nodavirus has become a serious pathogen for a wide range of cultured marine fish species. in the present work, the expression of genes related to immune and inflammatory responses of sea bream (sparus aurata l.), considered as non susceptible species, was studied both in vitro and in vivo. no replication of the virus was observed in head kidney macrophages and blood leukocytes. moreover, the enhancement of expression of several immune genes (tumor necrosis factor alpha (tnfalpha), interleukin-1- ...200817543386
temporal profile of brain and pituitary gnrhs, gnrh-r and gonadotropin mrna expression and content during early development in european sea bass (dicentrarchus labrax l.).a likely endocrine control mechanism for sexual differentiation in size-graded populations of european sea bass (dicentrarchus labrax) is proposed by evaluating the brain expression and pituitary content of two forms of gonadotropin-releasing hormone (gnrh), namely sea bream (sbgnrh) and salmon (sgnrh), the pituitary expression of one subtype of gnrh receptor (dlgnrh-r-2a) and the three gonadotropin (gth) subunits, namely glycoprotein alpha (gpalpha), follicle-stimulating hormone beta (fshbeta) ...200716962597
effects of gutting and ungutting on microbiological, chemical, and sensory properties of aquacultured sea bream (sparus aurata) and sea bass (dicentrarchus labrax) stored in ice.the effect of gutting and ungutting on microbiological, chemical, and sensory properties of aqua-cultured sea bream (sparus aurata) and sea bass (dicentrarchus labrax) stored in ice were studied. the total viable mesophilic and psychrophilic bacterial counts increased throughout the storage period of gutted and ungutted sea bream and sea bass. the mesophilic counts reached 8.19 log cfu/g for ungutted sea bream and 7.93 log cfu/g for ungutted sea bass after 14 days of storage. the mesophilic coun ...200616954060
intra-adrenal interactions in fish: catecholamine stimulated cortisol release in sea bass (dicentrarchus labrax l.).the effect of the catecholamines, adrenaline and noradrenaline, on sea bass (dicentrarchus labrax) and sea bream (sparus auratus) interrenal cortisol production was studied in vitro using a dynamic superfusion system technique. increasing concentrations of catecholamines (10(-6), 10(-8) and 10(-10) m) stimulated cortisol production in a dose-dependent manner, in sea bass only. the increase in cortisol production stimulated by adrenaline (10(-6) m) and noradrenaline (10(-6) m) was inhibited by so ...200616443381
parasite communities of adriatic cage-reared fish.from june 2001 to march 2002, 7 semi-offshore facilities in the adriatic sea rearing sea bass dicentrarchus labrax, sea bream sparus aurata, sharpsnout bream diplodus puntazzo and red sea bream pagellus bogaraveo aged 1+, were monitored for the presence of protozoan and metazoan parasites. obtained data sets of abundance and prevalence were used to evaluate the structure and dynamic of infra- and component parasite communities. in all hosts, except the red sea bream, dominant parasites were mono ...200515900691
long-term exposure to continuous light inhibits precocity in european male sea bass (dicentrarchus labrax, l.): hormonal aspects.the effect of long-term exposure to continuous light on the hormonal aspects of the reproductive axis was investigated in juvenile male sea bass (dicentrarchus labrax, l.) during the first annual cycle. four-month-old fish were exposed to a simulated natural photoperiod (np) and a continuous light (24 h) regime (ll) under natural conditions of temperature (13.3-25.8 degrees c). a dot-blot technique was used to analyse gonadotropin (the common glycoprotein alpha, gpalpha; the follicle stimulating ...200515613274
life cycle of ceratothoa oestroides, a cymothoid isopod parasite from sea bass dicentrarchus labrax and sea bream sparus aurata.ceratothoa oestroides (risso, 1826) (isopoda: cymothoida) is a protandric hermaphrodite parasite on a wide range of wild fish species. in recent years it has become a threat to cage-reared fish facilities, where high fish density provides optimal conditions for transmission. its impact on fish health and economical gain is significant, varying from growth retardation and decreased immunocompetency to direct loss due to mass mortalities of juvenile fishes. because of the sheltered location of the ...200314735926
muscle development in gilthead sea bream (sparus aurata, l.) and sea bass (dicentrarchus labrax, l.): further histochemical and ultrastructural aspects.the histochemical profiles--matpase and nadh-tr reactions--of the red and white muscle fibres of gilthead sea bream and sea bass were determined from the first week after hatching. modifications of the matpase technique by combinations of ph/time/molarity were carried out in order to compare the sensitivity of the myosin atpase of each muscle fibre type of the lateral muscle. results showed that the staining of muscle fibres was independent of small modifications in the technique. the intermedia ...19989741144
the post-larval development of lateral musculature in gilthead sea bream sparus aurata (l.) and sea bass dicentrarchus labrax (l.).fibre-type differentiation of lateral musculature has been studied in gilthead sea bream sparus aurata (l.) and sea bass dicentrarchus labrax (l.) during post-larval development using ultrastructural, histochemical and morphometric techniques. the study showed three muscle layers: red, intermediate (or pink) and white. initially, most of the red muscle showed low myosin atpase (m-atpase) activity fibres, whereas near the transverse septum some small high m-atpase activity fibres appeared and lat ...19989505442
amino acid composition and endocrine control of vitelline envelope proteins in european sea bass (dicentrarchus labrax) and gilthead sea bream (sparus aurata).the vitelline envelopes of european sea bass and gilthead sea bream are both composed of mainly four proteins with the molecular masses of 90, 52, 48, 45 kda and 75, 50, 48, 44 kda, respectively. each protein has an amino acid composition that is characterized by a high content of proline and glutamic acid and a low content of cysteine, similar to the whole vitelline envelope of both species. the amino acid composition suggests that each protein is distinct but related to the other vitelline env ...19958588933
quantitative immunoenzymatic detection of viral encephalopathy and retinopathy virus (betanodavirus) in sea bass dicentrarchus labrax.viral encephalopathy and retinopathy disease caused by betanodavirus, genus of the family nodaviridae, affects marine, wild and farmed species including sea bass, one of the most important farmed species in europe. this work describes a reliable and sensitive indirect elisa assay to detect betanodavirus in biological samples using a polyclonal antiserum (pab 283) against the 283/i09 virus strain, the most common red-spotted grouper nervous necrosis virus (rgnnv) genotype in the mediterranean are ...201626610431
radionuclides ((210)po and (210)pb) and some heavy metals in fish and sediments in lake bafa, turkey, and the contribution of (210)po to the radiation dose.the pollution level of lake bafa was investigated by collecting fish samples {dicentrarchus labrax (sea bass), liza ramada (mullet) and anguilla anguilla (eel)}, surface sediment, and core samples. in all these samples, (210)po and (210)pb concentrations were estimated, and total annual dose rates were obtained for each species. some heavy metal (cr, ni, pb, cd, mn, fe, and zn) concentration levels were obtained for the fish and a core sample. the sediment mass accumulation rate was found to be ...201627834886
rapid recovery following short-term acoustic disturbance in two fish species.noise from human activities is known to impact organisms in a variety of taxa, but most experimental studies on the behavioural effects of noise have focused on examining responses associated with the period of actual exposure. unlike most pollutants, acoustic noise is generally short-lived, usually dissipating quickly after the source is turned off or leaves the area. in a series of experiments, we use established experimental paradigms to examine how fish behaviour and physiology are affected, ...201626909193
pcb contamination in fish community from the gironde estuary (france): blast from the past.the contamination of the gironde estuary, southwest of france, by polychlorinated biphenyls (pcbs) was assessed using six fish of high ecological and economic importance as bioindicator species. the concentrations of 21 pcb congeners and total fat contents were determined in the muscle and liver of eels (anguilla anguilla), seabass (dicentrarchus labrax), flounders (platichthys flesus), meagres (argyrosomus regius), mullets (liza ramada), and soles (solea vulgaris). in addition, information rega ...201424238912
fish welfare assurance system: initial steps to set up an effective tool to safeguard and monitor farmed fish welfare at a company level.the objective was to take a first step in the development of a process-oriented quality assurance (qa) system for monitoring and safeguarding of fish welfare at a company level. a process-oriented approach is focused on preventing hazards and involves establishment of critical steps in a process that requires careful control. the seven principles of the hazard analysis critical control points (haccp) concept were used as a framework to establish the qa system. haccp is an internationally agreed ...201222278705
anguilla japonica lectin 1 delivery through adenovirus vector induces apoptotic cancer cell death through interaction with prmt5.our previous studies have demonstrated that, through adenovirus mediated gene delivery, various exogenously expressed lectins elicited cytotoxicity to cancer cells, utilizing protein arginine methyltransferase 5 (prmt5) as a common binding target.201626990556
acth response to lps in the first stages of development of the fish dicentrarchus labrax l.acth and acth receptor-like molecules were found at the examined stages of development (2, 4, 8, 12, 18, and 24 days post-hatching) in yolk sac, pronephros tubules, interrenal tissue, thymus, liver, spleen, cardinal veins, and skin of the teleost fish dicentrarchus labrax. acth and the related receptor-like molecules show a similar distribution. lps treatment at two different stages (8 and 24 days post-hatching) provoked both a release and an induction of acth-like molecules, suggesting an impor ...200516061067
an in vitro screening with emerging contaminants reveals inhibition of carboxylesterase activity in aquatic organisms.pharmaceuticals and personal care products (ppcps) form part of the new generation of pollutants present in many freshwater and marine ecosystems. although environmental concentrations of these bioactive substances are low, they cause sublethal effects (e.g., enzyme inhibition) in non-target organisms. however, little is known on metabolism of ppcps by non-mammal species. herein, an in vitro enzyme trial was performed to explore sensitivity of carboxylesterase (ce) activity of aquatic organisms ...201526562051
assessment of trace metal concentrations in muscle tissue of certain commercially available fish species from kayseri, turkey.regular consumption of fish has been widely recommended by health authorities. however, it is known that some species accumulate high levels of contaminants including heavy metals (e.g., hg, cd, pb, and as). in this study, cu, pb, co, ni, cr, mn, cd, and fe were determined in the muscle tissue of 11 commercially available fish species (sparus auratus, dicentrarchus labrax, mullus barbatus, belone belone, psetta maxima, epinephelus aeneus, salmothymus, soleidae, pomatomus saltatrix, engraulis enc ...201424633787
amyloodinum ocellatum in dicentrarchus labrax: study of infection in salt water and freshwater aquaponics.this investigation is aimed to improve the knowledge on the physiological alterations occurring at morphological and molecular level in european sea bass naturally infected by a. ocellatum and reared at different salinities. european sea bass juveniles (dicentrarchus labrax) weighing 20 ± 0.5 g were divided in three aquaponics systems: ctrl, reared at 20 ppt salinity; afi, reared in freshwater (0 ppt) and infected with the dinoflagellate amyloodinium ocellatum; asi, reared at 20 ppt salinity and ...201627542617
comparative pathogenicity study of ten different betanodavirus strains in experimentally infected european sea bass, dicentrarchus labrax (l.).viral encephalopathy and retinopathy (ver), otherwise known as viral nervous necrosis (vnn), is a severe pathological condition caused by rna viruses belonging to the nodaviridae family, genus betanodavirus. the disease, described in more than 50 fish species worldwide, is considered as the most serious viral threat affecting marine farmed species in the mediterranean region, thus representing one of the bottlenecks for further development of the aquaculture industry. to date, four different gen ...201423662921
influence of pesticides on the ph regulatory enzyme, carbonic anhydrase, from european seabass liver and bovine erythrocytes.the objective of this study was to assess the inhibitory effects of six commonly used pesticides, cyhalothrin, cypermethrin, dichlorvos, methamidophos, chlorpyrifos and methylparathion, on the ph regulatory enzyme carbonic anhydrase (ca) of dicentrarchus labrax (european seabass) liver (dca) and bovine erythrocytes (bca). results of the study showed that the pesticides displayed quite variable inhibition profiles with k(i) values ranging from 0.376 to 26.164μm against dca, and from 1.174 to 53.2 ...201222561108
orexins and receptor ox2r in the gastroenteric apparatus of two teleostean species: dicentrarchus labrax and carassius auratus.orexin a and b peptides and the receptor ox2r were studied in sea bass and goldfish gastroenteric tract by immunoblotting combined with densitometric analysis using nih image j software and immunohistochemical techniques. these teleost species present a different gut organization and diverse feeding habits. immunoblotting experiments showed one band of 16 kda corresponding to prepro-orexin, and one band of 38 kda corresponding to the ox2r receptor. immunohistochemical localization of oxa and oxb ...201627223125
immunohistochemical and immunochemical characterization of the distribution of leptin-like proteins in the gastroenteric tract of two teleosts (dicentrarchus labrax and carassius auratus l.) with different feeding habits.leptin is a modulator of food intake and energy homeostasis both in mammals and in some species of nonmammals vertebrates. in this study, we reported for the first time, using an immunohistochemical and immunochemical approach, the presence and distribution of immunoreactivity to leptin-like protein in the gastroenteric tract of dicentrarchus labrax (bass) and carassius auratus (goldfish), two teleostean species with different feeding and different adaptative morphological organization of the ga ...201120945462
molecular epidemiology of betanodaviruses isolated from sea bass and sea bream cultured along the tunisian coasts.viral nervous necrosis (vnn) is a serious viral disease affecting farmed sea bass (dicentrarchus labrax). only scarce molecular data are available on the disease-causing betanodavirus populations in tunisia. therefore, we carried out the first molecular survey of betanodaviruses in farmed sea bass and sea bream (sparus aurata) along the tunisian coasts. among 81 samples from five farms, 20 tested positive with rt-pcr, not only in clinical cases but also in asymptomatic fish before and during out ...201323288625
innervation and control of the adenohypophysis by hypothalamic peptidergic neurons in teleost fishes: em immunohistochemical evidence.previous light microscopic studies have revealed neuropeptide-immunoreactive neurosecretory fibers in the teleostean neurohypophysis, and ultrastructural work has reported direct innervation of endocrine cells by the terminals of fibers penetrating the adenohypophysis. this paper reviews our recent data from ultrastructural, immunohistochemical, receptor localization, and superfusion studies, which suggest a role for neuropeptides in the control of teleost pituitary secretion. we have used a com ...19999915561
high hydrostatic pressure influences the in vitro response to xenobiotics in dicentrarchus labrax liver.hydrostatic pressure (hp) increases by about 1 atmosphere (0.1mpa) for each ten-meter depth increase in the water column. this thermodynamical parameter could well influence the response to and effects of xenobiotics in the deep-sea biota, but this possibility remains largely overlooked. to grasp the extent of hp adaptation in deep-sea fish, comparative studies with living cells of surface species exposed to chemicals at high hp are required. we initially conducted experiments with precision-cut ...201626836508
presence of trace metals in aquaculture marine ecosystems of the northwestern mediterranean sea (italy).information regarding chemical pollutant levels in farmed fish and shellfish, along with the risks associated with their consumption is still scarce. this study was designed to assess levels of exposure to 21 trace elements in fish (dicentrarchus labrax), mussels (mytilus galloprovincialis) and oysters (crassostrea gigas) collected from aquaculture marine ecosystems of the northwestern mediterranean sea. metal concentrations showed great variability in the three species; the highest values of th ...201627179326
sequence comparison and phylogenetic analysis of fish nodaviruses based on the coat protein gene.we have amplified by reverse transcription-polymerase chain reaction (rt-pcr) and sequenced a 605-bp fragment covering the variable region of the coat protein gene of fish nodaviruses infecting european sea bass, dicentrarchus labrax (n = 24), and shi drum, umbrina cirrosa (n = 2), in the mediterranean basin. nine new isolates were identified and their sequences were combined with sequences in the literature to produce three different data sets. the first set, based on amino acid sequences, was ...200111504420
immune effects of hfo on european sea bass, dicentrarchus labrax, and pacific oyster, crassostrea gigas.the european sea bass, dicentrarchus labrax, and the pacific oyster, crassostrea gigas, were exposed to a soluble fraction of heavy fuel oil for 5 and 9 days, respectively. the organisms were then transferred to non-contaminated seawater for 1 month. the bioaccumulation and elimination of pahs in contaminated tissues were dissimilar between species. in fish, acenaphthene and naphthalene were detected and naphthalene was still detectable 30 days after the beginning of the recovery period. in oyst ...200919406476
receptor specificity and functional comparison of recombinant sea bass (dicentrarchus labrax) gonadotropins (fsh and lh) produced in different host systems.different yields, biopotency, and in vivo pharmacokinetics are obtained for recombinant sea bass gonadoltropins depending on the production system and dna construct, but they show specific activation of their corresponding receptors. gonadotropins (gths) are glycoprotein hormones that play a major role in the regulation of gonadal functions. recently, we succeeded in isolating the native sea bass fsh from sea bass pituitaries, but to ensure the availability of bioactive gths and no cross-contami ...201121293031
cytokine expression in head-kidney leucocytes of european sea bass (dicentrarchus labrax l.) after incubation with the probiotic vagococcus fluvialis l-21.the european sea bass (dicentrarchus labrax l.) is one of the most extensively farmed marine fish in the mediterranean sea. under the high-density condition, common in aquaculture, the infectious diseases can cause significant economic losses. probiotics are presented as an alternative to antibiotics for the control of aquaculture diseases. this study used real-time pcr to investigate in vitro the dynamic of expression of immune-related genes in sea bass after incubation with live and inactivate ...201323927874
the in vitro effect of probiotic vagococcus fluvialis on the innate immune parameters of sparus aurata and dicentrarchus labrax.in this study we evaluated the effect of the probiotic vagococcus fluvialis on the cellular immune unspecific system of two different fish species of great interest in aquaculture such as gilthead sea bream (sparus aurata) and european sea bass (dicentrarchus labrax). leucocytes from head kidney of the two fish species were extracted and concentration adjusted to 10(7) cells ml(-1). phagocytic and respiratory burst activity and the peroxidase content of leucocytes were observed 30 min after incu ...201222864109
synthetic hepcidin from fish: uptake and protection against vibrio anguillarum in sea bass (dicentrarchus labrax).the generation of a variety of new therapeutic agents to control and reduce the effects of pathogen in aquaculture is urgently needed. the antimicrobial peptides (amps) are one of the major components of the innate defenses and typically have broad-spectrum antimicrobial activity. however, absorption and distributions of exogenous amps for therapeutics application on farmed fish species need to be studied. previous studies in our laboratory have shown the properties of hepcidin as an effective a ...201627368538
antibacterial and anti-paf activity of lipid extracts from sea bass (dicentrarchus labrax) and gilthead sea bream (sparus aurata).the anti-paf and the antibacterial activities of lipid extracts obtained from cultured sea bass (dicentrarchus labrax) and cultured gilthead sea bream (sparus aurata) were evaluated. total lipids of sea bass and gilthead sea bream exerted paf-like activity while, in higher amounts they inhibited this paf activity. neutral lipids of both sea bass and gilthead sea bream contained only paf antagonists while the polar lipid fractions contained both paf antagonists and agonists. total lipids of sea b ...200826047447
interactions between candidate probiotics and the immune and antioxidative responses of european sea bass (dicentrarchus labrax) larvae.the use of lactic acid bacteria (lab) as probiotics in aquaculture may improve the quality of seed production and limit the use of antibiotics in fish hatcheries. this study attempted to further characterize the candidate probiotic lactobacillus casei x2, and the immune and physiological responses of the sea bass larvae. l. casei x2 was confirmed as a good candidate, due to its wide antibacterial spectrum against both gram-positive and gram-negative bacteria, and its free radical scavenging acti ...201627133829
characterization of european sea bass transcripts by rna seq after oral vaccine against v. anguillarum.the european sea bass (dicentrarchus labrax) is, along with the gilthead sea bream (sparus aurata), one of the most extensively cultured species in european aquaculture productions. massive mortalities may be caused by bacterial or viral infections in intensive aquaculture production. evaluation of the efficacy of an oral vaccine against vibrio anguillarum (aquavac vibrio oral) in sea bass revealed specific immune gene expression profiles in the gut as well as protection of fish. in the present ...201222790793
effects on mortality and stress response in european sea bass, dicentrarchus labrax (l.), fed mannan oligosaccharides (mos) after vibrio anguillarum exposure.the effects of dietary mannan oligosaccharides (mos; 4 g kg(-1) ; bio-mos, alltech inc, usa) in diets for european sea bass, dicentrarchus labrax (l.), juveniles in relation to disease and stress resistance, combining intestinal infection with vibrio anguillarum and stress challenge by confinement, were assessed in this study. after 8 weeks of mos supplementation, fish were exposed to a pathogen challenge test against v. anguillarum by direct gut inoculation combined with a confinement stressor ...201222690841
treatment of vibriosis in european sea bass larvae, dicentrarchus labrax l., with oxolinic acid administered by bath or through medicated nauplii of artemia franciscana (kellogg): efficacy and residual kinetics.european sea bass larvae were challenged by bath with listonella anguillarum strain 332a, 2.5×10(7) cfuml(-1) for 1h. fish either received no treatment or oral treatment with artemia franciscana (kellog) nauplii enriched with oxolinic acid, or bath treatments with oxolinic acid. medication commenced 1day following challenge and was performed on days 1, 3 and 5 post-challenge at a dosage of 20mgl(-1) for 2h for bath treatments, while two doses each of 750 nauplii per fish were administered daily ...201222670590
transcription of histones h1 and h2b is regulated by several immune stimuli in gilthead seabream and european sea bass.histones (h1 to h4) are the primary proteins which mediate the folding of dna into chromatin; however, and in addition to this function, histones have been also related to antimicrobial peptides (amps) activity in vertebrates, in fact, mammalian h1 is mobilized as part as the anti-viral immune response. in fish, histones with amp activity have been isolated and characterized mainly from skin and gonads. one of most threatening pathogens for wild and cultured fish species nowadays is nodavirus (n ...201627523279
isolation of lacinutrix venerupis strains associated with disease outbreaks in sea bream sparus aurata and european sea bass dicentrarchus labrax.four gram-negative bacterial isolates were recovered from 2 disease outbreaks that occurred in 2013 affecting european sea bass dicentrarchus labrax fry and sea bream sparus aurata adults. main symptoms were erratic swimming, eroded fins and, in the sea bream outbreak, haemorrhages on the body surface; bacteria were always recovered from internal organs, almost in pure culture. on the basis of phenotypic characterization and 16s rrna gene sequence analysis, the isolates were identified as lacinu ...201728357988
description and comparative study of physico-chemical parameters of the teleost fish skin mucus.the study of mucosal surfaces, and in particular the fish skin and its secreted mucus, has been of great interest recently among immunologists. measurement of the viscosity and other physico-chemical parameters (protein concentration, ph, conductivity, redox potential, osmolality and density) of the skin mucus can help to understand its biological functions. we have used five marine species of teleost: gilthead seabream (sparus aurata l.), european sea bass (dicentrarchus labrax l.), shi drum (u ...201526484392
shift in trophic level of mediterranean mariculture species.the mean trophic level of the farmed fish species in the mediterranean has been increasing. we examined the farming-up hypothesis (i.e., the increase in the production of high-trophic-level species) in the mediterranean by determining the trophic level of the aquafeeds (i.e., what the fish are fed) of 5 species of farmed marine fishes: common dentex (dentex dentex), common pandora (pagellus erythrinus), european seabass (dicentrarchus labrax), gilthead seabream (sparus aurata), and red porgy (pa ...201424628519
ozone disinfection of eggs from gilthead seabream sparus aurata, sea bass dicentrarchus labrax, red porgy, and common dentex dentex dentex.the risk of fish pathogen transmission via eggs can be reduced by disinfection in ozonated seawater. the aim of this study was to determine the suitable conditions for ozone disinfection of the eggs of gilthead seabream sparus aurata, sea bass dicentrarchus labrax, red porgy pagrus pagrus, and common dentex dentex dentex. the eggs were disinfected with a concentration (c) of 0.5 mg of ozone/l of water at four different exposure times (t = 2, 4, 8, and 16 min). the hatching rate was determined in ...201222838084
α-msh and melanocortin receptors at early ontogeny in european sea bass (dicentrarchus labrax, l.).temporal patterns of whole-body α-msh concentrations and of transcripts of melanocortin receptors during early development as well as the endocrine response (α-msh, cortisol, mcr mrnas) to stress at the end of the larval period were characterized in dicentrarchus labrax. immunohistochemistry showed α-msh positive cells in the pituitary pars intermedia in all stages examined. as development proceeds, α-msh content gradually increases; mrna levels of mc2r and mc4r remain low until first feeding wh ...201728378841
localised residency and inter-annual fidelity to coastal foraging areas may place sea bass at risk to local depletion.for many marine migratory fish, comparatively little is known about the movement of individuals rather than the population. yet, such individual-based movement data is vitally important to understand variability in migratory strategies and fidelity to foraging locations. a case in point is the economically important european sea bass (dicentrarchus labrax l.) that inhabits coastal waters during the summer months before migrating offshore to spawn and overwinter. beyond this broad generalisation ...201728374772
vitamin d3 affects innate immune status of european sea bass (dicentrarchus labrax l.).the effects of vitamin d3 dietary administration on certain innate immune parameters on the expression of immune-related genes in head-kidney (hk) and gut were investigated in european sea bass dicentrarchus labrax. vitamin d3 (vd3) was orally administered to fish in a commercial pellet food supplemented with 0 (control); 3750; 18,750; or 37,500 u kg(-1). furthermore, gut histology was considered. this study showed a modulation in the activities examined in fish fed with the addition of vd3. aft ...201728374186
supplementation of arachidonic acid rich oil in european sea bass juveniles (dicentrarchus labrax) diets: effects on leucocytes and plasma fatty acid profiles, selected immune parameters and circulating prostaglandins levels.the main objective of this study was to assess the effects of graded levels of dietary arachidonic acid (ara), supplemented from alternative sources, on fatty acid composition of plasma and head kidney leucocytes of european sea bass (dicentrarchus labrax). for that purpose, sea bass juveniles were fed four diets containing graded levels of ara as follows: 0.5% (ara0.5), 1% (ara1), 2% (ara2) and 4% (ara4) during 60 days. at the end of the feeding trial fatty acid profiles of plasma and head kidn ...201728359945
impact of temperature on sea bass, dicentrarchus labrax, retina: fatty acid composition, expression of rhodopsin and enzymes of lipid and melatonin metabolism.teleost fish are ectothermic vertebrates. their metabolism, physiology and behavior rely on the external temperature. this study, in the retina of the sea bass dicentrarchus labrax, reports on the impact of temperature on the fatty acids composition and mrna abundance of key enzymes of the lipid metabolism: fatty acid desaturase-2 (fads2), fatty acid elongase-5 (elovl5), sterol regulatory element-binding protein-1 (srebp-1), lipase and phospholipase a2 (pla2). we also report on the effects on th ...201728347705
marine lectins dlfbl and hddsbl fused with soluble coxsackie-adenovirus receptor facilitate adenovirus infection in cancer cells but have different effects on cell survival.cancer development and progression are usually associated with glycosylation change, providing prognostic and diagnostic biomarkers, as well as therapeutic targets, for various cancers. in this work, dicentrarchus labrax fucose binding lectin (dlfbl) and haliotis discus discus sialic acid binding lectin (hddsbl) were genetically fused with soluble coxsackie-adenovirus receptor (scar), and produced through a bacterial expression system. results showed that recombinant scar-dlfbl not only facilita ...201728335432
immunoglobulin t from sea bass (dicentrarchus labrax l.): molecular characterization, tissue localization and expression after nodavirus infection.immunoglobulins (igs) are fundamental components of the adaptive immune system of vertebrates, with the igt/igz isotype specific of teleosts. in this paper we describe the identification of an igt heavy chain from the european sea bass (dicentrarchus labrax l.), its molecular characterization and tissue mrna localization by in situ hybridization.201728298204
ecophysiological responses of juvenile seabass (dicentrarchus labrax) exposed to increased temperature and dietary methylmercury.the ecotoxicological effects of methylmercury (mehg) exposure have been intensively described in literature. yet, it is still unclear how marine biota will respond to the presence of mehg under climate change, namely ocean warming. the present study aimed to investigate, for the first time, fish condition [fulton's k index (k), hepatosomatic index (his) and brain-to-body mass ratio (bb-ratio)] and several stress-related responses in an ecologically and commercially important fish species (dicent ...201728216029
effects of pinealectomy on the neuroendocrine reproductive system and locomotor activity in male european sea bass, dicentrarchus labrax.the seasonally changing photoperiod controls the timing of reproduction in most fish species, however, the transduction of this photoperiodic information to the reproductive axis is still unclear. this study explored the potential role of two candidate neuropeptide systems, gonadotropin-inhibitory hormone (gnih) and kisspeptin, as mediators between the pineal organ (a principle transducer of photoperiodic information) and reproductive axis in male european sea bass, dicentrarchus labrax. two sev ...201728188883
transcriptomic profiling of egg quality in sea bass (dicentrarchus labrax) sheds light on genes involved in ubiquitination and translation.variable and low egg quality is a major limiting factor for the development of efficient aquaculture production. this stems from limited knowledge on the mechanisms underlying egg quality in cultured fish. molecular analyses, such as transcriptomic studies, are valuable tools to identify the most important processes modulating egg quality. however, very few studies have been devoted to this aspect so far. within this study, the microarray-based transcriptomic analysis of eggs (of different quali ...201728181038
evolution of lymphocytes. immunoglobulin t of the teleost sea bass (dicentrarchus labrax): quantitation of gene expressing and immunoreactive cells.immunoglobulin t (igt) is one of the key effector molecules of jawed vertebrate's adaptive immune system, and in this work we describe the quantitative distribution of igt-expressing and igt-producing cells in tissues of the european seabass dicentrarchus labrax by using mrna riboprobes and a specific anti-igt antibody. a polyclonal antiserum (pab) was prepared by immunizing rabbits with three synthetic peptides deduced from the full length igt cdna sequence and located in a surface-exposed ch3 ...201728167252
influence of green tides in coastal nursery grounds on the habitat selection and individual performance of juvenile fish.coastal ecosystems, which provide numerous essential ecological functions for fish, are threatened by the proliferation of green macroalgae that significantly modify habitat conditions in intertidal areas. understanding the influence of green tides on the nursery function of these ecosystems is essential to determine their potential effects on fish recruitment success. in this study, the influence of green tides on juvenile fish was examined in an intertidal sandy beach area, the bay of saint-br ...201728125605
developmental changes and day-night expression of the gonadotropin-inhibitory hormone system in the european sea bass: effects of rearing temperature.the role of rearing temperature on fish development, sex differentiation and puberty has been largely addressed, but the impact of water temperature on the ontogeny of the main neuroendocrine systems controlling reproduction has received little attention. gonadotropin-inhibitory hormone (gnih) has been shown to act on gonadotropin-releasing hormone (gnrh) neurons and on the pituitary to inhibit gonadotropin release and synthesis in vertebrates, including sea bass, dicentrarchus labrax. in the pr ...201728109838
molecular characterization and quantification of estrogen receptors in turbot (scophthalmus maximus).estrogens regulate various reproductive processes via estrogen receptor (er)-mediated signaling pathway in vertebrates. in this study, full-length sequences coding for erα, erβ1 and erβ2 were isolated from female turbot (scophthalmus maximus) by homology cloning and a strategy based on rapid amplification of cdna end-polymerase chain reaction (race-pcr). the nucleotide and amino acid sequences of turbot ers showed high homologies with the corresponding sequences of other fish species and signifi ...201728087301
european sea bass show behavioural resilience to near-future ocean acidification.ocean acidification (oa)-caused by rising concentrations of carbon dioxide (co2)-is thought to be a major threat to marine ecosystems and has been shown to induce behavioural alterations in fish. here we show behavioural resilience to near-future oa in a commercially important and migratory marine finfish, the sea bass (dicentrarchus labrax). sea bass were raised from eggs at 19°c in ambient or near-future oa (1000 µatm pco2) conditions and n = 270 fish were observed 59-68 days post-hatch using ...201628018656
heritability of boldness and hypoxia avoidance in european seabass, dicentrarchus labrax.to understand the genetic basis of coping style in european seabass, fish from a full factorial mating (10 females x 50 males) were reared in common garden and individually tagged. individuals coping style was characterized through behavior tests at four different ages, categorizing fish into proactive or reactive: a hypoxia avoidance test (at 255 days post hatching, dph) and 3 risk-taking tests (at 276, 286 and 304 dph). we observed significant heritability of the coping style, higher for the a ...201627992517
the tongue dorsal surface in fish: a comparison among three farmed species.the oral cavity of vertebrates has a very important role in many functions strictly related to the food processing. two species, sea bass dicentrarchus labrax and seabream sparus aurata, are mainly involved in the aquaculture industry in the mediterranean area; moreover, the white seabream diplodus sargus sargus was recently chosen as the best candidate for the diversification of species of commercial interest. this investigation was carried out, using the standard procedures for light and scann ...201727990675
an integrated genomic approach for the study of mandibular prognathism in the european seabass (dicentrarchus labrax).skeletal anomalies in farmed fish are a relevant issue affecting animal welfare and health and causing significant economic losses. here, a high-density genetic map of european seabass for qtl mapping of jaw deformity was constructed and a genome-wide association study (gwas) was carried out on a total of 298 juveniles, 148 of which belonged to four full-sib families. out of 298 fish, 107 were affected by mandibular prognathism (mp). three significant qtls and two candidate snps associated with ...201627929136
dispersed oil decreases the ability of a model fish (dicentrarchus labrax) to cope with hydrostatic pressure.data on the biological impact of oil dispersion in deep-sea environment are scarce. hence, the aim of this study was to evaluate the potential interest of a pressure challenge as a new experimental approach for the assessment of consequences of chemically dispersed oil, followed by a high hydrostatic pressure challenge. this work was conducted on a model fish: juvenile dicentrarchus labrax. seabass were exposed for 48 h to dispersant alone (nominal concentration (nc) = 4 mg l(-1)), mechanically ...201727854059
the european seabass (dicentrarchus labrax) innate immunity and gut health are modulated by dietary plant-protein inclusion and prebiotic supplementation.inclusion of prebiotics in aqua feeds, though a costly strategy, has increased as a means to improve growth. still, its effects on health improvement are not fully disclosed. regarding their immunestimulatory properties, research has focused on carbohydrates such as fructooligosaccharides and xylooligosaccharides demonstrating their modulatory effects on immune defences in higher vertebrates but few studies have been done on their impact on fish immunity. replacing fish meal (fm) by plant protei ...201727836721
changes in the fish assemblages of a coastal lagoon subjected to gradual salinity increases.this study analyses fish data to understand how the gradual increase of salinity registered in a coastal lagoon and consequently, anthropogenic disturbance, affected the fish communities. for that, fish assemblages of the ria de aveiro were sampled monthly in 3 years from different decades (1988, 1997 and 2012). dominant species were atherina boyeri, a. presbyter, sardina pilchardus, dicentrarchus labrax, liza aurata and l. ramada. significant differences in fish communities were detected among ...201627836187
the highly variable microbiota associated to intestinal mucosa correlates with growth and hypoxia resistance of sea bass, dicentrarchus labrax, submitted to different nutritional histories.the better understanding of how intestinal microbiota interacts with fish health is one of the key to sustainable aquaculture development. the present experiment aimed at correlating active microbiota associated to intestinal mucosa with specific growth rate (sgr) and hypoxia resistance time (hrt) in european sea bass individuals submitted to different nutritional histories: the fish were fed either standard or unbalanced diets at first feeding, and then mixed before repeating the dietary challe ...201627821062
foxl3, a target of mir-9, stimulates spermatogenesis in spermatogonia during natural sex change in monopterus albus.two foxl2 paralogs, foxl2 (also named foxl2a) and foxl3 (also named foxl2b), were considered as fish-specific duplicates. both belong to the forkhead box family of transcription factors, which play important roles in regulating reproduction involved in sexual differentiation and regulation of primordial germ cell and gonadal somatic cell development. we isolated the complete foxl3 cdna from the rice field eel (monopterus albus), which undergoes a natural female-to-male sex change via an intersex ...201627583788
testicular steroidogenesis and locomotor activity are regulated by gonadotropin-inhibitory hormone in male european sea bass.gonadotropin-inhibitory hormone (gnih) is a neurohormone that suppresses reproduction by acting at both the brain and pituitary levels. in addition to the brain, gnih may also be produced in gonads and can regulate steroidogenesis and gametogenesis. however, the function of gnih in gonadal physiology has received little attention in fish. the main objective of this study was to evaluate the effects of peripheral sbgnih-1 and sbgnih-2 implants on gonadal development and steroidogenesis during the ...201627788270
early life low intensity stress experience modifies acute stress effects on juvenile brain cell proliferation of european sea bass (d. labrax).early life adversity may be critical for the brain structural plasticity that in turn would influence juvenile behaviour. to address this, we questioned whether early life environment has an impact on stress responses latter in life, using european sea bass, dicentrarchus labrax, as a model organism. unpredictable chronic low intensity stress (uclis), using a variety of moderate intensity stressors, was applied during two early ontogenetic stages, flexion or formation all fins. at juvenile stage ...201727638037
photoperiod modulates the reproductive axis of european sea bass through regulation of kiss1 and gnrh2 neuronal expression.the onset of puberty is characterized by activation of the brain-pituitary-gonad axis. however, the molecular and endocrine mechanism involved in the process of puberty and the influence of environmental conditions, such as photoperiod signalling, are not well understood in fish. in this study, 1-year-old male european sea bass (dicentrarchus labrax) were exposed to photoperiod manipulation in combination with size-sorting. two treatment groups, a puberty accelerating photoperiod (ap) group and ...201727641683
establishment of a new teleost brain cell line (dlb-1) from the european sea bass and its use to study metal toxicology.in teleost fish, there are no commercial cell lines for the european sea bass (dicentrarchus labrax). thus, we have established the sea bass brain (dlb-1) cell line, using a fish retrovirus for immortalization, which resemble epithelial cells and express glial cells markers. exposure to metals [cd, methylmercury (mehg), pb or as] produces cytotoxicity and induction of reactive oxygen species (ros) production. interestingly, cell cycle analysis of dlb-1 cells shows that exposure to metals alters ...201727746373
water temperature influences growth and gonad differentiation in european sea bass (dicentrarchus labrax, l. 1758).the effect of rearing temperature on gonad differentiation and growth was evaluated in sea bass (dicentrarchus labrax). one control group (cg, n = 60) and two experimental groups (eg1, n = 30; eg2, n = 30) were selected. cg was reared at 15.0 °c during 10 days post-hatch (dph) and at 19.0 °c throughout the remaining larval and post-larval development. eg1 was exposed to 14.5 °c from 1 to 50 dph, followed by an increase to 20 °c until sampling (176 dph). eg2 was exposed to 14.5 °c from 1 to 37 dp ...201727751603
comparing the genotoxicity of a potentially carcinogenic and a noncarcinogenic pah, singly, and in binary combination, on peripheral blood cells of the european sea bass.research on the toxicological mechanisms of polycyclic aromatic hydrocarbons (pahs) deemed carcinogenic and noncarcinogenic has mostly been developed for individual compounds even though, in the environment, pahs invariably occur in mixtures. the present work aimed at understanding the interaction effects of two model pahs, the potentially carcinogenic benzo[b]fluoranthene (b[b]f) and the noncarcinogenic phenanthrene (phe) to a marine fish (the sea bass dicentrarchus labrax). the study endeavour ...201625728603
having a blast: searchable transcriptome resources for the gilthead sea bream and the european sea bass.the gilthead sea bream (sparus aurata) and the european sea bass (dicentrarchus labrax) are the most important aquaculture species in the mediterranean sea and since the last decade it has been seen an exponential increase in their available molecular resources. in order to improve accessibility to transcriptome resources, expressed sequence tags (ests), mrna sequences and raw read sequences were assembled and deposited in blast queryable databases. the publicly available sea bream and sea bass ...201627742405
gill morphology in two mediterranean sea fishes of similar feeding preferences: sea bream (sparus aurata l) and sea bass (dicentrarchus labrax).the aim of the present study was to describe the gill morphology of the gilthead sea bream and sea bass fishes that showed the same feeding habits. our study focused on the gross morphological, scanning and light microscopical features of the gills, where a many differences in the gill arches and gill rakers surface in both species were explained. our results marked that; there was an interbranchial median elevation appeared in sea bream. the gill arches length and the gaps between decreased med ...201323475751
elemental composition of four farmed fish produced in portugal.farmed gilthead sea bream (sparus aurata), european sea bass (dicentrarchus labrax), rainbow trout (oncorhynchus mykiss) and turbot (psetta maxima) produced in portugal were analysed in order to characterize their elemental composition. atomic absorption (flame and cold vapour) and molecular absorption spectrometry techniques were used to determine all the studied elements. similar patterns of macro, trace and ultra trace elements were observed for all fish species. the main elements were potass ...201222574998
discriminating farmed gilthead sea bream sparus aurata and european sea bass dicentrarchus labrax from wild stocks through scales and otoliths.the study of mass and standard length (l(s) ) relationships showed that farmed individuals had higher values than wild fishes for both gilthead sea bream sparus aurata and european sea bass dicentrarchus labrax. such differences were more pronounced in larger individuals than smaller ones and were more noticeable in s. aurata than in d. labrax. additionally, differences in external characteristics of scales were detected between origins. a high proportion of farmed s. aurata had a regenerated nu ...201222551175
effects of olive pomace and olive pomace oil on growth performance, fatty acid composition and cardio protective properties of gilthead sea bream (sparus aurata) and sea bass (dicentrarchus labrax).gilthead sea bream (sparus aurata) and sea bass (dicentrarchus labrax) were fed with two experimental diets: olive pomace diet and olive pomace oil diet, in order to examine whether fish oil substitution in fish feeds by olive oil production wastes, such as olive pomace and olive pomace oil, has an influence on growth performance, fatty acid composition and cardio protective properties of fish. gilthead sea bream fed with both experimental diets exhibited satisfactory growth performance factors ...201125212344
a microsatellite linkage map of striped bass (morone saxatilis) reveals conserved synteny with the three-spined stickleback (gasterosteus aculeatus).the striped bass (morone saxatilis) and its relatives (genus morone) are of great importance to fisheries and aquaculture in north america. as part of a collaborative effort to employ molecular genetics technologies in striped bass breeding programs, we previously developed nearly 500 microsatellite markers. the objectives of this study were to construct a microsatellite linkage map of striped bass and to examine conserved synteny between striped bass and three-spined stickleback (gasterosteus a ...201221968826
response to short term starvation of growth, haematological, biochemical and non-specific immune parameters in european sea bass (dicentrarchus labrax) and blackspot sea bream (pagellus bogaraveo).growth, haematological (haematocrit), biochemical (serum cortisol and glucose), and non-specific immune (lysozyme, serum haemolytic and haemagglutinating activities, extracellular respiratory burst activity) parameters, were monitored in european sea bass dicentrarchus labrax and blackspot sea bream pagellus bogaraveo subjected to a 31 days starvation compared to fed fish, to assess the responses to feed deprivation of these health status indicators. while haematocrit, serum cortisol, glucose an ...201121664688
functional divergences of gapdh isoforms during early development in two perciform fish species.glyceraldehyde-3-phospate dehydrogenase (gapdh) is involved in basic cell catabolic processes and, as it is thought to be continuously expressed, belongs to the group of housekeeping genes. thus, it is frequently used as an internal control in quantitative gene expression studies. however, the evidence of different expression patterns in a broad range of organisms and tissues, as well as the occurrence of different isoforms, shows that gapdh has to be reevaluated as an internal control in qpcr s ...201121559871
cellular responses in the skin of the gilthead sea bream sparus aurata l. and the sea bass dicentrarchus labrax (l.) exposed to high ammonia.adult gilthead sea bream sparus aurata and sea bass dicentrarchus labrax were exposed for 24 and 48 h, respectively, to two concentrations of ammonia each (mean values of 3·34 and 13·10 mg l(-1) ta-n in s. aurata; 2·99 and 11·90 mg l(-1) ta-n in d. labrax). light microscopy and computerized morphometry were used to evaluate ammonia-induced alterations in skin structure during exposure and following recovery in normal water. in s. aurata, ammonia exposure induced a concentration-dependent increas ...201121463312
quantitative trait loci involved in sex determination and body growth in the gilthead sea bream (sparus aurata l.) through targeted genome scan.among vertebrates, teleost fish exhibit a considerably wide range of sex determination patterns that may be influenced by extrinsic parameters. however even for model fish species like the zebrafish danio rerio the precise mechanisms involved in primary sex determination have not been studied extensively. the zebrafish, a gonochoristic species, is lacking discernible sex chromosomes and the sex of juvenile fish is difficult to determine. sequential protandrous hermaphrodite species provide disti ...201121304996
effects of garlic and ginger oils on hematological and biochemical variables of sea bass dicentrarchus labrax.this study was conducted to investigate the effects of garlic and ginger oils on hematological and biochemical health characteristics of sea bass dicentrarchus labrax. fish were exposed to garlic oil (0.01 or 0.02 ml/l), ginger oil (0.01 or 0.02 ml/l), or a combination of the two oils (each oil at a concentration of 0.005 or 0.01 ml/l) for 96 h via bath immersion. results showed that the red blood cell count, hematocrit (%), hemoglobin (hb) concentration (g/dl), mean corpuscular volume (μm(3)), ...201223043295
uncommon functional properties of the first piscine 26s proteasome from the antarctic notothenioid trematomus bernacchii.protein homoeostasis is a fundamental process allowing the preservation of functional proteins and it has a great impact on the life of the antarctic organisms. however, the effect of low temperatures on protein turnover is poorly understood and the cold-adaptation of the degradation machinery remains an unresolved issue. as the 26s proteasome represents the main proteolytic system devoted to the controlled degradation of intracellular proteins, the purpose of the present study was to investigat ...201626933238
distribution of ghrelin peptide in the gastrointestinal tract of stomachless and stomach-containing teleosts.the occurrence and localization of ghrelin peptide in the gastrointestinal tract of carassius auratus and dicentrarchus labrax, two fish species that exhibit different feeding behavior, different habitats, and different anatomical organizations of the gastroenteric tract, were examined by immunohistochemical methods and western blotting analysis. all of the gastrointestinal segments studied displayed immunohistochemical localizations of ghrelin peptide. numerous single or clustered immunoreactiv ...200919322896
toxicity evaluation of diethylene glycol and its combined effects with produced waters of off-shore gas platforms in the adriatic sea (italy): bioassays with marine/estuarine species.diethylene glycol (deg) is commonly used to dehydrate natural gas in off-shore extraction plants and to prevent formation of gas hydrates. it may be released into the sea accidentally or in discharged produced waters (pws). pws samples from off-shore gas platforms in the adriatic sea (italy) have been used in this study. the objectives of the study were: a) to evaluate the toxicity of deg for marine organisms; b) to evaluate if a high deg content in pws may alter their toxicity; c) to verify whe ...201222245535
retrograde positive contrast urethrocystography of the fish urogenital system.the radiological differences between the urinary tract of  dicentrarchus labrax, sparus aurata, tinca tinca, and cyprinus carpio are shown. in fresh water teleosts the urinary bladder is sigmoid and a short urethra leads to the urinary pore. genital and anal pores are present. in sparus aurata the urinary bladder has a globoid shape. in dicentrarchus labrax the urinary bladder is smaller and elongate. in both marine teleosts a single urogenital pore is visible. positive contrast was used to surv ...201425180198
apoptosis in thymus of teleost fish.the presence and distribution of apoptotic cells during thymus development and in adult were studied by in situ end-labelling of fragmented dna in three temperate species carp (cyprinus carpio), sea bass (dicentrarchus labrax) and dusky grouper (epinephelus marginatus) and in the adult thymus of three antarctic species belonging to the genus trematomus spp. during thymus development some few isolated apoptotic cell (ac) firstly appeared in the central-external part of the organ (carp: 5 days ph; ...201323639932
phylogeny of cytokines: molecular cloning and expression analysis of sea bass dicentrarchus labrax interleukin-1beta.in this paper the cloning of interleukin-1beta (il-1beta) from the fish dicentrarchus labrax (sea bass) is described. using degenerate primers designed from known il-1beta sequences, a cdna fragment was amplified by pcr and elongated by 3' and 5' race to give the full-length coding sequence for sea bass il-1beta. the cdna is 1292 bp, lacks a putative ice cut site, and codes for a deduced peptide of 29.4 kda with a pi of 5.1. sequence analysis showed highest amino acid similarity with rainbow tro ...200111759041
invertebrate and fish cytokines.cytokine-like molecules are well described in invertebrates, although most recent studies have revealed that there is analogy, rather than homology, between invertebrate and vertebrate cytokine-like activities. cytokines certainly appeared early in the evolution of vertebrates, dating back some 400 millions years. here, evidence will be reviewed and updated of the presence of these molecules in jawed fish and in particular, in bony fish, which represent the oldest group displaying true functiona ...200011022118
presence of tonofilaments and thymic serum factor (fts) in thymic epithelial cells of a fresh water fish (carp: cyprinus carpio) and a sea water fish (bass: dicentrarchus labrax).using indirect immunofluorescence we demonstrated the presence of fts in two fishes; the size of epithelial cells of the carp is smaller than of the bass.19863798525
anti-genotoxic ability of α-tocopherol and anthocyanin to counteract fish dna damage induced by musk xylene.many compounds released into the environment are able to interact with genetic material. the main purpose of genetic toxicology is to investigate the adverse effects of genotoxic molecules such as reduced fitness, changes in gene frequencies and their impact on genetic diversity in populations following genotoxic exposure. however, the ecological effects of many genotoxic compounds remain poorly understood. the aim of this research was to evaluate the genotoxic activity of an artificial musk (mu ...201526407710
somatotropic axis genes are expressed before pituitary onset during zebrafish and sea bass development.the somatotropic axis, or growth hormone-insulin-like growth factor-1 (gh-igf-1) axis, of fish is involved in numerous physiological process including regulation of ionic and osmotic balance, lipid, carbohydrate and protein metabolism, growth, reproduction, immune function and behavior. it is thought that gh plays a role in fish development but conflicting results have been obtained concerning the ontogeny of the somatotropic axis. here we investigated the developmental expression of gh, gh-rece ...201324055560
comparative analysis of intronless genes in teleost fish genomes: insights into their evolution and molecular function.this study assessed the relationship between the occurrence and function of intronless or single exon genes (seg) in the genome of five teleost species and their phylogenetic distance. the results revealed that takifugu rubripes, tetraodon nigroviridis, oryzias latipes, gasterosteus aculeatus and danio rerio genomes are respectively comprised of 2.83%, 3.42%, 4.49%, 4.35% and 4.02% segs. these segs encode for a variety of family proteins including claudins, olfactory receptors and histones that ...201121620332
different phylogenomic approaches to resolve the evolutionary relationships among model fish species.comparative genomics holds the promise to magnify the information obtained from individual genome sequencing projects, revealing common features conserved across genomes and identifying lineage-specific characteristics. to implement such a comparative approach, a robust phylogenetic framework is required to accurately reconstruct evolution at the genome level. among vertebrate taxa, teleosts represent the second best characterized group, with high-quality draft genome sequences for five model sp ...201020591844
functional expression of the oligopeptide transporter pept1 from the sea bass (dicentrarchus labrax).complementary rna, derived from the intestine of the sea bass dicentrarchus labrax and putatively coding for a ph-dependent oligopeptide transporter pept1 (slc15 family), was injected in xenopus oocytes that were subsequently tested with electrophysiological techniques. transport-associated currents were observed when various di- or tripeptides were applied at concentrations ranging between 0.1 and 10 mm. no currents were generated by histidine nor by other single amino acids. sea bass pept1 als ...200919618206
climate influence on juvenile european sea bass (dicentrarchus labrax, l.) populations in an estuarine nursery: a decadal overview.estuarine systems support the life cycle stages of commercially important marine fish and are influenced by large and local-scale climatic patterns. in this study, performed in the mondego estuary, portugal, we used an 11-year database (2003-2013) for analyzing the variability in the population of a marine juvenile migrant fish, the european sea bass dicentrarchus labrax, regarding changes in abundance, population structure, growth rates, secondary production and annual day of peak abundance. hi ...201627720527
repeatability of cortisol stress response in the european sea bass (dicentrarchus labrax) and transcription differences between individuals with divergent responses.understanding the stress responses of organisms is of importance in the performance and welfare of farmed animals, including fish. especially fish in aquaculture commonly face stressors, and better knowledge of their responses may assist in proper husbandry and selection of breeding stocks. european sea bass (dicentrarchus labrax), a species with high cortisol concentrations, is of major importance in this respect. the main objectives of the present study were to assess the repeatability and con ...201627703277
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