| hepatic disease associated with intrauterine parvovirus b19 infection in a newborn premature infant. | a hydropic premature infant with intrauterine growth retardation died at 4 days of age and was found at necropsy to have advanced liver disease. clinical and serologic findings in mother and infant were consistent with recent parvovirus b19 infection. parvovirus can cause fetal liver disease in animals, and some instances of congenital hepatic dysfunction in man may be due to intrauterine parvovirus b19 infection. | 1989 | 2550601 |
| human parvovirus b19 and hydrops fetalis. | | 1988 | 2893952 |
| fetal cell tropism of human parvovirus b19. | | 1988 | 2895293 |
| human parvovirus b19 infection in pregnancy. | | 1988 | 2900943 |
| anti-dna and antilymphocyte antibodies during acute infection with human parvovirus b19. | sera from patients in the acute and recovery stages of parvovirus b19 infection, and from individuals with no detectable antiparvovirus antibody were examined for the presence of anti-dna and antilymphocyte antibodies. sixty-eight percent of individuals recently recovered from parvovirus infection had elevated levels of antidouble stranded (ds) and antisingle stranded (ss) dna antibodies. in addition, a cytotoxic igm antilymphocyte antibody was detected in more than 88% of these same sera. seria ... | 1989 | 2550630 |
| human parvovirus (b19) and erythema infectiosum. | an outbreak of erythema infectiosum ("fifth disease") was studied in fukuoka, japan, in 1980-1981. human parvovirus (hpv) antigen was not detected in any patients, but anti-hpv, measured by countercurrent immunoelectrophoresis, was found in 33 of 34 affected children and in 21 (15%) of 141 children of the same ages without the disease. immunoglobulin m class anti-hpv was present in all 25 children with erythema infectiosum tested. in a survey of hospital patients, the prevalence of anti-hpv dete ... | 1985 | 2989471 |
| [aplastic crisis due to human parvovirus b19 infection in glucose-6-phosphate dehydrogenase deficiency]. | human parvovirus b19 is known to cause aplastic crisis in patients with hemolytic anemias due to cytotoxic effect of the infection to erythroid progenitor cells. we report here the first case of aplastic crisis by b19 in a patient with glucose-6-phosphate dehydrogenase deficiency. a five-year-old boy was admitted to the hospital because of severe anemia, fever and jaundice. four weeks after admission, he developed erythema infectiosum. b19 infection was confirmed using countercurrent immunoelect ... | 1989 | 2559569 |
| embryonic malformations in a case of intrauterine parvovirus b19 infection. | an incomplete embryo of 9 weeks development from a woman infected by human parvovirus b19 during early pregnancy was histologically examined. b19-dna was detected in both embryonic and placental tissue by dot-blot hybridization. focal vascular endothelial damage was found throughout the entire embryo and placenta together with mononuclear infiltrations around the vessels. in the placenta these mononuclear cells belonged for the greater part to the cytotoxic and/or suppressor t-cell group. one ey ... | 1989 | 2543097 |
| [human parvovirus b19]. | | 1986 | 3020445 |
| [parvovirus infections in dermatology]. | human parvovirus b19 is the causative agent of erythema infectiosum and other atypical (rubelliform) exanthemas. on account of the relatively high prevalence of b19, these infections are rather frequent. they usually occur during childhood, but also up to the age of 30, and there are various clinical manifestations. during pregnancy, b19 infection may cause hydrops of the fetus with ensuing fetal death. anti-b19 igm and igg can be detected by means of elisa or ria; b19-dna is identified by hybri ... | 1989 | 2544061 |
| human parvovirus b19 infection associated with vascular purpura and vasculitis. | | 1989 | 2544532 |
| aplastic crisis caused by b19 virus in a child during induction therapy for acute lymphoblastic leukemia. | a child undergoing induction therapy for acute lymphoblastic leukemia suffered an aplastic crisis associated with b19 virus infection. good response to the antiblastic therapy led to a burst in erythropoiesis favoring high viral replication, which was responsible for strong erythroblastic inhibition and severe viremia. the patient's b19 antibody response became evident very late, probably because of the antiblastic effect of the therapy; nevertheless, recovery was complete in little more than a ... | 1989 | 2545576 |
| infection with human parvovirus (b19), aplasia of the bone marrow and a rash in hereditary spherocytosis. | infection with human parvovirus (hpv) b19 was diagnosed in persons with hereditary spherocytosis during an outbreak of erythema infectiosum. a 10-year-old boy had an aplastic crisis of the bone marrow and a maculopapular rash. his mother also had an aplastic crisis, fever, rash and a transiently acellular marrow. another boy had an aplastic crisis, leucopenia and fever, but did not have a rash. aplastic crisis and a rash have rarely before been observed together and attributed to infection with ... | 1987 | 3029235 |
| [acute parvovirus b19 arthritis]. | | 1987 | 3029747 |
| infection control measures for human parvovirus b19 in the hospital setting. | | 1989 | 2545772 |
| serological study of rubella-like illnesses. | we investigated 627 patients who within a period of 2 1/2 years had had a rubelliform rash and/or symptoms of arthritis and arthralgia. sera from these patients were investigated for evidence of rubella, human parvovirus b19 (hpv), and measles infection with methods to detect specific igm and igg antibodies. complement fixation tests were used to screen for a wide range of other infectious agents. we detected 229 cases of rubella, 43 cases of hpv infection, 7 cases of measles, and 9 cases of inf ... | 1987 | 3035074 |
| human parvovirus b19 infection during pregnancy. | human parvovirus b19 (b19) has been implicated as the cause of fifth disease and has been associated with fetal death. we identified pregnant women who were at risk of contracting b19 infection during an outbreak of fifth disease. the sera of 12 women classified at high risk of exposure and 19 classified at low risk were tested during prenatal care, at delivery, or at both times for igg and igm antibodies to b19. four women at high risk but none at low risk were considered infected because they ... | 1987 | 3036955 |
| [parvovirus b19 infections. outbreak in a medical technology educational institute]. | | 1987 | 3038495 |
| erythema infectiosum in a primary school: investigation of an outbreak in bury. | between january and april 1987, 97 of 302 (32%) children attending a primary school in bury, lancashire, were affected by an exanthematous illness. the duration and extent of the episode caused considerable local interest and anxiety. examination of 6 serum specimens obtained during the subsequent investigation demonstrated evidence of recent human parvovirus b19 infection in 3 children and 1 teacher. no evidence of recent rubella virus infection was found. the investigation of the episode is di ... | 1989 | 2552489 |
| a prospective study of acute idiopathic neuropathy. ii. antecedent events. | the incidence of antecedent events and serological evidence of preceding infection were studied in 100 patients with acute idiopathic neuropathy and age and sex matched control subjects in south-east england. symptoms of respiratory infections occurred within one month before onset of neuropathic symptoms in 38% of patients and 12% of controls (p less than 0.001) and symptoms of gastrointestinal infections in 17% of patients and 3% of controls (p less than 0.005). immunisations, insect bites and ... | 1988 | 3404161 |
| transient erythroblastopenia of childhood due to human parvovirus b19 infection. | | 1989 | 2553096 |
| characterization of capsid and noncapsid proteins of b19 parvovirus propagated in human erythroid bone marrow cell cultures. | the major capsid and noncapsid proteins of the pathogenic parvovirus b19, propagated in vitro, were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoprecipitation, and immunoblot of the erythroid fraction of infected human bone marrow cell cultures. there were two capsid proteins of 58 kilodaltons (kda; the major species) and 84 kda (the minor species). newly synthesized capsid viral proteins were present in the supernatants of infected cultures. the major noncapsid p ... | 1987 | 3599184 |
| detection of parvovirus b19 dna, antigen, and particles in the human fetus. | human parvovirus b19 commonly infects children, causing erythema infectiosum (fifth disease). however, there is a significant adult population which has not been exposed to the virus and, consequently, does not have protective antibody. recent reports have associated b19 infection during pregnancy with fetal death, although normal outcome of pregnancy is more common. to characterise further the role of b19 infection in fetal deaths, a series of laboratory investigations has been undertaken on ti ... | 1987 | 3694180 |
| nucleotide sequence and genome organization of human parvovirus b19 isolated from the serum of a child during aplastic crisis. | the nucleotide sequence of an almost-full-length clone of human parvovirus b19 was determined. whereas the extreme left and right ends of this genomic clone are incomplete, the sequence clearly indicates that the two ends of viral dna are related by inverted terminal repeats similar to those of the dependovirus genus. the coding regions are complete in the cloned dna, and the two large open reading frames which span almost the entire genome are restricted to one strand, as has been found for all ... | 1986 | 3701931 |
| complete nucleotide sequence and genome organization of bovine parvovirus. | we determined the complete nucleotide sequence of bovine parvovirus (bpv), an autonomous parvovirus. the sequence is 5,491 nucleotides long. the terminal regions contain nonidentical imperfect palindromic sequences of 150 and 121 nucleotides. in the plus strand, there are three large open reading frames (left orf, mid orf, and right orf) with coding capacities of 729, 255, and 685 amino acids, respectively. as with all parvoviruses studied to date, the left orf of bpv codes for the nonstructural ... | 1986 | 3783814 |
| a transcriptional promoter of the human parvovirus b19 active in vitro and in vivo. | the human parvovirus b19 causes aplastic crises in sickle cell anemia patients and the disease erythema infectiosum. so far, it has not been possible to grow b19 virus in cultured cells. here we report the use of in vitro transcription in hela cell extracts and transient expression of cloned dna transfected into hela cells to detect and map a strong transcriptional promoter on the b19 genome. the promoter is located near the left end of the b19 genome, at position 6 map units in the clone pyt103 ... | 1987 | 3824910 |
| detection of human parvovirus using a molecularly cloned probe. | half of the genomic dna of the human parvovirus (b19) was cloned in the plasmid pbr322. the cloned dna was used as a molecular probe for the detection of parvovirus in serum by means of a dot hybridization test. in an assay of 26 samples, the dot hybridization test was found to be of comparable sensitivity and to be as rapid as radioimmunoassay for viral antigen detection; it is potentially useful as a diagnostic test. | 1985 | 3973569 |
| parvovirus b19 finds a disease. | | 1981 | 6117755 |
| nakatani antigen and human parvovirus (b19) | | 1984 | 6140462 |
| screening of blood donors for human parvovirus b19. | | 1995 | 7475701 |
| concurrent outbreaks of influenza and parvovirus b19 in a boys' boarding school. | in the spring term of 1985 there was a protracted outbreak of upper respiratory tract febrile illness consistent with a clinical diagnosis of influenza in a boys' boarding school, which lasted from 23 january to 29 march. although influenza virus infection was confirmed in 89% of cases in the first half of the term, 53% of the cases which occurred in the second half of the term had no evidence of infection with influenza virus. between 5 february and 31 march 28 boys presented with skin rashes c ... | 1989 | 2553464 |
| human parvovirus b19-induced aplastic crisis in autoimmune haemolytic anaemia. | | 1988 | 2839223 |
| pancytopenia secondary to parvovirus b19 infection in a child with acute lymphoblastic leukemia, mimicking leukemic relapse. | | 1995 | 7485090 |
| persistent parvovirus b19 infection and pure red cell aplasia in waldenström's macroglobulinemia: successful treatment with high-dose intravenous immunoglobulin. | | 1995 | 7485092 |
| human parvovirus infection in haemophiliacs first infused with treated clotting factor concentrates. | a group of 27 first infused haemophiliacs was studied for association between heat-treated clotting factor concentrates and transmission of human parvovirus b19. the prevalence rate of b19 antibody, detected by the immunoelectroosmophoresis (ieop) reaction, was 55.5% in this group of first infused subjects, significantly higher than the 29.3% of the control group of 58 healthy blood donors but lower than the 93.3% of antibody positive subjects in a group of 30 haemophiliacs multitreated with unh ... | 1988 | 2839609 |
| [parvovirus b19 infection in different shapes. but more laboratories have refined the diagnostic methods]. | | 1995 | 7490957 |
| virucidal short wavelength ultraviolet light treatment of plasma and factor viii concentrate: protection of proteins by antioxidants. | the use of solvent/detergent mixtures and various forms of heat treatment to inactivate viruses has become widespread in the preparation of blood derivatives. because viruses that lack lipid envelopes and/or are heat resistant, eg, hepatitis a virus (hav) or parvovirus b19 may be present, the use of two methods of virus elimination that operate by different mechanisms has been advocated. we now report on short wavelength ultraviolet light (uvc) irradiation for virus inactivation and enhancement ... | 1995 | 7492794 |
| electron microscopic identification of parvovirus virions in erythroid-line cells in fatal hydrops fetalis. | intrauterine infection with parvovirus b19 may lead to fatal hydrops fetalis. intranuclear particles, consistent with parvovirus virions, within erythroid cells were readily identified on transmission electron microscopy of formalin-preserved material obtained at necropsy from a neonate and two fetuses in whom clinical and light microscopic criteria for parvovirus b19 infection were met. no such particles were seen in similar material from a neonate and two fetuses with erythroblastosis fetalis ... | 1988 | 2842740 |
| a simple and sensitive dna hybridization assay used for the routine diagnosis of human parvovirus b19 infection. | a dot blot hybridization assay for parvovirus b19 diagnosis was developed by using a pcr-generated probe, digoxigenin labelling, and chemiluminescence detection. different labelling techniques and hybridization solutions were evaluated. from this analysis a protocol was devised for routine diagnostic use. the protocol enabled 1 pg of b19 dna to be detected. the results of applying this method to 8,369 diagnostic samples collected during 1994 and 1995 are given. | 1995 | 7494051 |
| human parvovirus b19: elisa and immunoblot assays. | an elisa for the detection of specific igm and igg against human parvovirus b19 (anti-b19 igm and igg) and b19 antigen is described. with elisa anti-b19 igm could be detected for up to 20 weeks after viraemia. four to five months after b19 infection anti-b19 igg titres range between 10(-6) and 10(-7). nonspecific reactions with rheumatoid factor or igm against rubella were not found. the elisa for b19 antigen was shown to be as sensitive as dna hybridisation. with immunoblotting two viral protei ... | 1988 | 2843558 |
| [the osteoarticular manifestations associated with parvovirus b19 infection in the course of an infectious outbreak]. | | 1995 | 7494445 |
| b19 virus--a pathogenic human parvovirus. | b19 virus is the first human virus to be shown to be a member of the parvovirus genus. this review is concerned with the diseases associated with b19 virus, their nature, pathogenesis and diagnosis. the virus was discovered by chance in blood donors but has been shown to be a common infection of childhood. infection may be asymptomatic or associated with mild, non-specific symptoms. the most common specific clinical manifestation is an erythematous rash illness which often has the classical feat ... | 1987 | 2844330 |
| human parvovirus b19-induced transient pancytopenia in a child with hereditary spherocytosis. | we report a child with hereditary spherocytosis who developed human parvovirus b19 (hpv b19)-induced transient pancytopenia. symptoms of acute-phase illness subsided promptly after infusion of gammaglobulin containing hpv b19 antibody. the hpv b19-containing serum on admission inhibited not only erythroid colony growth but also myeloid and megakaryocyte colony growths. the inhibitory effect was abrogated by convalescent-phase serum or gammaglobulin solution containing hpv b19 antibody. these fin ... | 1988 | 2846031 |
| parvovirus infections. from benign to life-threatening. | erythema infectiosum, aplastic crisis, intrauterine fetal demise, and an acute polyarthralgia syndrome can all be caused by human parvovirus b19. the major pathophysiologic effect of infection seems to be cessation of erythropoiesis as a result of cytolytic infection of red cell precursors. erythema infectiosum is a benign disease that usually occurs in childhood. the acute polyarthralgia syndrome is likewise benign, and patients in whom parvovirus infection has been confirmed by serologic testi ... | 1988 | 2847129 |
| [serological diagnosis for human parvovirus b19 infection by an enzyme immunoassay kit with recombinant antigens synthesized in a baculovirus expression system]. | propagation of human parvovirus b19 (b19) in cell cultures are not applicable to the source of viral antigens for serological assays at present. enzyme immunoassay (eia) kits with recombinant b19 capsids by e. coli or baculovirus expression system have been developed. we tested serum samples from the patients with erythema infectiosum and aplastic crisis by eia kit with recombinant antigens synthesized in a baculovirus expression system (denka seiken co., tokyo, japan). the antigens used in the ... | 1995 | 7499916 |
| screening blood donations for parvovirus b19. | | 1995 | 7500773 |
| [transient erythroblastopenia in 4 children]. | in four patients, all girls, aged 2, 3.5, 4 and 5 years, transient erythroblastopenia was diagnosed. the children were presented because of acute pallor. the haemoglobin levels were 2.8 to 5.0 mmol/l. after 3 weeks all patients had recovered or were recovering with increasing haemoglobin values. three of the four patients needed one blood transfusion. in two patients there was evidence of a parvovirus b19 infection. transient erythroblastopenia is mostly seen in patients aged 1-4 years. most cas ... | 1995 | 7501078 |
| chimeric parvovirus b19 capsids for the presentation of foreign epitopes. | chimeric proteins consisting of the vp2 capsid protein of human parvovirus b19 and defined linear epitopes from human herpes simplex virus type 1 and mouse hepatitis virus a59 inserted at the n-terminus and at a predicted surface region were expressed by recombinant baculoviruses. the chimeric proteins expressed the inserted epitopes and assembled into empty capsids. immunoelectron microscopy indicated that the epitopes inserted in the loop were exposed on the surface of the chimeric particles. ... | 1994 | 7507280 |
| parvovirus b19 associated with pseudoappendicitis. | | 1987 | 2892027 |
| childhood transient erythroblastopenia complicated by thrombocytopenia and neutropenia. | we report on 4 children with transient erythroblastopenia complicated by thrombocytopenia and/or neutropenia. bone marrow examination revealed severe erythroid hypoplasia with normal granulopoiesis and thrombopoiesis. human parvovirus b19 infection was confirmed serologically in 2 children. an in vitro study using autologous bone marrow cells after recovery demonstrated igg-mediated inhibition of erythropoiesis in 4 children. additionally, antibodies directed against platelets and neutrophils we ... | 1989 | 2914597 |
| secondary structures of lipid-associating peptides: a fourier transform infrared study. | four peptides from 20 to 28 residues in length were studied by fourier transform infrared (ftir) spectroscopy in solution and in complexes with dimyristoylphosphatidylcholine (dmpc). the four peptides included the 20-residue lipid-associating peptide, lap-20, which was predicted to form an amphipathic helical structure in the presence of lipids, and three other peptides whose sequences had less amphipathic helix-forming properties. the complexes were shown by electron microscopy to be discoidal ... | 1994 | 7516748 |
| human parvovirus b19-induced epidemic acute red cell aplasia in patients with hereditary hemolytic anemia. | from march to august 1984, 26 patients with hereditary hemolytic anemia in northeastern ohio developed acute, profound red cell aplasia. the patients included 14 males and 12 females 2 to 23 years old, with sickle cell anemia (20 cases), hemoglobin sc-disease (4 cases), sickle-beta-thalassemia (1 case), or hereditary spherocytosis (1 case). all had an acute onset of severe reticulocytopenia and anemia and prodromal symptoms of illness including fever, abdominal symptoms, headache, and arthralgia ... | 1986 | 3008891 |
| the role of parvovirus b19 in aplastic crisis and erythema infectiosum (fifth disease). | in 1984, simultaneous outbreaks of aplastic crisis and erythema infectiosum occurred in northeastern ohio. sera were analyzed from 26 patients with aplastic crisis: 24 had igm specific for parvovirus b19, five had b19-like particles by electron microscopy, and 13 had dna from b19; no sera from 33 controls had evidence of recent infection with b19 (p less than .0001). dna from b19 was also detected in specimens of throat gargle and urine from two patients with aplastic crisis. sera from 36 of 51 ... | 1986 | 3016109 |
| transient expression of b19 parvovirus gene products in cos-7 cells transfected with b19-sv40 hybrid vectors. | hybrid b19 parvovirus-sv40 origin vectors were transfected into cos-7 cells and replication of these plasmids studied. plasmids that have a frameshift mutation within the nonstructural gene region replicated to high level (copy number approximately 10,000/transfected cell) although somewhat lower than psvod, the sv40 origin vector without b19 sequence (copy number approximately 100,000/transfected cell). however, hybrid b19 parvovirus-sv40 origin vectors that do not contain these frameshift muta ... | 1989 | 2800343 |
| [incidence of parvovirus b19 infection. seroepidemiologic studies]. | anti-b19-igg antibodies were tested for by the elisa method in 768 sera, 76 from children and juveniles, aged 1-15 years, attending the outpatients department of the children's clinic, university of munich, and 692 from persons, aged 18-68 years, attending the blood donor service of the bavarian red cross in munich. 38.4% of sera were positive, with a significant difference between men and women (32.5% vs. 47.5%, p less than or equal to 0.01). a fresh b19 infection was present in 42 subjects by ... | 1987 | 2820687 |
| antibodies to parvovirus b19 ns-1 protein in infected individuals. | human parvovirus b19 is the aetiological agent of the common childhood disease erythema infectiosum (fifth disease). the infection is usually benign and self-limiting, but in adults cases of severe arthritis which may persist for years have been reported. neutralizing antibodies directed against the structural proteins are usually produced shortly after the infection. the immune response against the third major protein, the non-structural protein ns-1, of parvovirus b19 has not been characterize ... | 1995 | 7534811 |
| [association of non-immunologically-induced hydrops fetalis with parvovirus b19 infection]. | five weeks after the onset of clinical symptoms of erythema infectiosum, a 24 year old pregnant woman, gravida i, para 0, suffered a hydrops fetalis which resulted in fetal loss. the maternal infection with the human parvovirus b19 could be confirmed serologically with elisa and immunoblot by detecting specific igm antibodies against b19. in an amniotic sample of the fetus parvovirus b19 dna could be detected by hybridisation. fetal loss was obviously due to a parvovirus b19 infection of the mot ... | 1987 | 2820831 |
| most of the vp1 unique region of b19 parvovirus is on the capsid surface. | b19 parvovirus is pathogenic in man and a vaccine is desirable. in convalescence after acute infection, the dominant humoral immune response is directed to the minor capsid protein called vp1, which differs from the major capsid protein by an additional nh2-terminal 227 amino acids. we have previously shown that this unique region contains multiple linear neutralizing epitopes. we produced seven recombinant b19 capsids that contained progressively truncated vp1 unique region sequences, each fuse ... | 1995 | 7544049 |
| viral arthritis including hiv. | viruses are attractive candidates for infectious etiologic agents or cofactors in the development of rheumatic diseases. the epidemic of hiv infection and the recognition of "emerging viruses" continues to fuel interest in the possible role of viruses in the pathogenesis of diseases without defined etiologies. during 1994, roles for parvovirus b19 in vasculitis and erosive rheumatoid arthritis were entertained. we were reminded that rubella infection may present with polyarthritis. our understan ... | 1995 | 7547112 |
| detection of antibodies and antigens of human parvovirus b19 by enzyme-linked immunosorbent assay. | acute-phase serum from a patient with aplastic crisis provided sufficient human parvovirus b19 to make a monoclonal antibody against b19 and to develop antigen and immunoglobulin m (igm) and igg antibody detection enzyme-linked immunosorbent assays (elisas). the indirect capture antibody method was used for all three assays. antigen was detected in 8 of 29 sera drawn within 2 days of onset of illness from patients with aplastic crisis. these sera had high titers of virus by electron microscopy a ... | 1986 | 3021807 |
| identification of the major structural and nonstructural proteins encoded by human parvovirus b19 and mapping of their genes by procaryotic expression of isolated genomic fragments. | plasma from a child with homozygous sickle-cell disease, sampled during the early phase of an aplastic crisis, contained human parvovirus b19 virions. plasma taken 10 days later (during the convalescent phase) contained both immunoglobulin m and immunoglobulin g antibodies directed against two viral polypeptides with apparent molecular weights of 83,000 and 58,000 which were present exclusively in the particulate fraction of the plasma taken during the acute phase. these two protein species comi ... | 1986 | 3021988 |
| human parvovirus b19 in pregnancy. | human parvovirus is an often unrecognized but potentially deadly infection when contracted by childbearing women who are seronegative. although maternal symptoms and sequelae are mild, the exposed fetus quickly can develop nonimmune hydrops fetalis and die. health care providers can prepare for appropriate maternal and fetal management by reviewing the history, disease process, and transmission mode of parvovirus. | 1995 | 7562131 |
| human parvovirus infection in early rheumatoid and inflammatory arthritis. | evidence of recent infection with human parvovirus b19 (hpv) was found in two patients with early rheumatoid arthritis (ra) and in four patients with acute inflammatory arthritis (ia). both of the patients with ra but only one of the four patients with ia carried ra associated haplotypes. no evidence of persistent infection with hpv was found, but evidence of past infection with hpv was significantly more common in patients with ra than in controls. the results confirm the arthritogenic potentia ... | 1986 | 3024592 |
| variation of erythroid and myeloid precursors in the marrow and peripheral blood of volunteer subjects infected with human parvovirus (b19). | infection of normal individuals with human parvovirus (b19) results in a mild disease (erythema infectiosum) but gives rise to aplastic crises in patients with chronic hemolytic anemias. the effects of this disease on hemopoiesis were investigated following intranasal inoculation of the virus into three volunteers. a typical disease ensued with a viremia peaking at 9 d. marrow morphology 6 d after inoculation appeared normal but at 10 d there was a severe loss of erythroid precursors followed by ... | 1987 | 3033026 |
| [blood transmission and infections]. | a blood transfusion can never become a completely risk free event. almost all kinds of infectious agents; viruses, bacteria and parasites, can be transmitted by blood. so far, hepatitis and hiv-infections have been focused. the state of readiness to meet these infections must be kept while we prepare for "new" agents, like parvovirus b19. extensive international travelling will increase the possibility of blood-borne parasitic infections, like malaria and chagas' disease, even with the very high ... | 1995 | 7570535 |
| the prevalence of antibody to human parvovirus b19 in england and wales. | the prevalence of antibody to human parvovirus b19 (anti-b19 igg) in england and wales was measured by an antibody-capture radioimmunoassay. over 2000 sera were examined; 1422 from the general population, 374 from unselected children admitted to hospital and 300 from women attending an antenatal clinic. waning levels of maternally-derived antibody were found in infants under 1 year old. in children 1-5 years old, 5-15% had anti-b19 igg and this rose to 50-60% in older children, young adults and ... | 1988 | 3339634 |
| human parvovirus b19-induced vesiculopustular skin eruption. | erythema infectiosum, fifth disease, is a usually benign macular or maculopapular exanthem of childhood caused by the human parvovirus b19. a 27-year-old woman with a serologically documented human parvovirus infection who presented with a hemorrhagic exanthem and enanthem with areas of pustules and pseudo-pustules is described. the histologic findings were unusual because they combined the histologic features of morbilliform and vesiculopustular viral lesions. this case serves to underscore the ... | 1988 | 2834947 |
| acquired pure red cell aplasia: a study of six cases. | persistent infection by parvovirus b19 associated with pure red cell aplasia (prca) has been documented in immunocompromised patients. bone marrow failure is associated with conditions in which immune surveillance is impaired, and in these instances occult parvovirus infection may be suspected. in this study we have assessed by serological and molecular methods whether parvovirus b19 infection may be a more frequent cause of prca than hitherto suspected and whether it may be present in the absen ... | 1995 | 7578524 |
| childhood exanthems. | childhood exanthems are a common cause of skin disease in children. several new aspects of exanthems are reviewed. two conditions, unilateral laterothoracic exanthem and asymmetric periflexural exanthem, may be variations of the same disease and could potentially represent a cutaneous reaction to several infectious agents. an eruption that has been called papular-purpuric gloves and socks syndrome is usually, but not invariably, caused by parvovirus b19 infection. another unusual exanthem, erupt ... | 1995 | 7581645 |
| fetal infection with human parvovirus b19. | the case of premature infant with nonimmune hydrops secondary to human parvovirus b19 infection is presented. the infection was not suspected clinically. the fetal autopsy showed intranuclear inclusions in erythroid cells that stained positively with a dna hybridization probe for b19 parvovirus. | 1988 | 2835306 |
| kawasaki disease associated with parvovirus b19 infection. | in a 9-week-old boy with kawasaki disease an active parvovirus b19 infection was diagnosed serologically. conclusion: this case supports the hypothesis of an aetiological relationship between parvovirus b19 infection and kawasaki disease. | 1995 | 7588963 |
| hydrops fetalis caused by intrauterine human parvovirus infection. | during a large outbreak of erythema infectiosum in 1987 in toyama prefecture, japan, a 32-year-old woman acquired a mild rash on her arms and legs at 18 weeks of gestation. at 26 weeks and 4 days of gestation, the fetus died by hydrops fetalis and pregnancy was terminated. histological studies of the fetus revealed degeneration of erythroblastic cells in the liver and bone marrow. extensive extramedullary hematopoiesis and hemosiderin deposits were observed in the liver. antibody response to hum ... | 1987 | 2838664 |
| novel transcription map for the b19 (human) pathogenic parvovirus. | the b19 parvovirus, a small single-stranded dna virus of 5.4 kilobases, is pathogenic in humans. b19 has remarkable specificity for erythroid progenitor cells and has been propagated in vitro only with human erythroid bone marrow. replication of viral dna and the viral protein products of b19 appear similar to those of other animal parvoviruses. however, b19 transcription had unusual features in comparison with that in other animal parvoviruses. at least nine overlapping poly(a)+ transcripts wer ... | 1987 | 3599180 |
| parvovirus b19 as a possible causative agent of fulminant liver failure and associated aplastic anemia. | we recently observed that more than one third of pediatric patients who presented with non-a, non-b fulminant liver failure (flf) also developed aplastic anemia (aa) either before or shortly after liver transplantation. factors involved in the suppression of bone marrow could be the same as those causing hepatic failure. we considered parvovirus b19 a candidate etiologic agent because of the known tropism of b19 for erythroid precursors. archived liver and serum from six patients undergoing live ... | 1995 | 7489971 |
| development and evaluation of capture immunoglobulin g and m hemadherence assays by using human type o erythrocytes and recombinant parvovirus b19 antigen. | the capacity of human parvovirus b19 to agglutinate human type o erythrocytes was used to develop immunoglobulin g and m antibody capture hemadherence assays. when results of these assays were compared with those of corresponding antibody capture enzyme immunoassays using a well-characterized panel of 125 serum specimens, a 96.8% overall agreement was obtained between the two methods. | 1995 | 7494048 |
| [studies on the relationship between anaphylactoid purpura and human parvovirus b19]. | human parvovirus b19 (b19) has been shown to be the cause of erthema infectiousum (ei). recently the various clinical manifestations with b19 infection are coming to light. the relationship between b19 and anaphylactoid purpura (schölein-henoch purpura: shp) was investigated in a retrospective study. there were sixteen patients (male 9, female 7, average age 6.0 +/- 1.7) who were diagnosed as shp. serum specimens were used in the present study. specific igm and igg were assayed by enzyme immunoa ... | 1995 | 7594788 |
| a new genome type of human parvovirus b19 present in sera of patients with encephalopathy. | the possible involvement of human parvovirus b19 infection in encephalopathy has been reported. to determine the characteristics of b19 viruses involved in such cases, we molecularly cloned a part of the b19 dnas derived from the sera of three patients with encephalopathy (b19 strains n80, n81 and n82), following amplification by pcr. the nucleotide (nt) sequences of the cloned dnas (nt 3147-3405) were then determined. the nucleotide sequence of n80 was similar to that of the known genome type o ... | 1995 | 7595371 |
| transcriptional transactivation of parvovirus b19 promoters in nonpermissive human cells by adenovirus type 2. | the pathogenic human parvovirus b19 contains a promoter at map unit 6 (b19p6) of the viral genome, expression from which is largely restricted to human cells in the erythroid lineage, whereas a putative promoter at map unit 44 (b19p44) is inactive during a natural viral infection. although nonerythroid human cells, such as hela and kb, allow expression from the b19p6 promoter but not from the b19p44 promoter following dna-mediated transfection, little expression from the b19p6 promoter occurs fo ... | 1995 | 7494329 |
| application of a low-light imaging device and chemiluminescent substrates for quantitative detection of viral dna in hybridization reactions. | in this quantitative dot-blot hybridization assay for detecting b19 parvovirus dna, we used three different chemiluminescent substrates [adamantyl-1,2-dioxetane phenyl phosphates (ppd and the new ppd-plus) and the chloro-5-substituted adamantyl-1,2-dioxetane phosphate (cspd) plus emerald enhancer] and a high-performance, low-intensity-light imaging luminograph apparatus. the hybridization test uses digoxigenin-labeled dna probes, which are immunoenzymatically revealed by anti-digoxigenin fab fra ... | 1995 | 7497607 |
| life-threatening human parvovirus b19 infection in immunocompetent haemophilia. | | 1995 | 7605434 |
| purification of functional recombinant yy1 expressed in sf9 insect cells. | we expressed a gene for yy1, the multifunctional mammalian transcription factor, with a recombinant baculovirus in insect cells and obtained high levels of protein in cell lysates. a simple and efficient purification method was developed. in the final step, we used dna affinity chromatography with concatermerized sequence derived from the upstream region of the b19 parvovirus p6 promoter, which has strong affinity for yy1. approximately 1.8 mg of highly purified yy1 was obtained from 10(9) sf9 c ... | 1995 | 7606165 |
| extent of sequence variability in a genomic region coding for capsid proteins of b19 parvovirus. | genomic variability in human parvovirus b19 was analysed by direct partial sequencing of different isolates collected in italy between 1989 and 1994. dna was purified from viremic serum samples and the region of viral genome coding for structural proteins was amplified by polymerase chain reaction and partially sequenced (nt. 2400-3400). data were compared to reference isolates wi (u.k., 1973) and au (u.s.a., 1982). the average relative distance between isolates was estimated at the low level of ... | 1995 | 7611882 |
| [parvovirus b19--a ubiquitous, little know virus. from erythema infectiosum and arthropathy to chronic anemia]. | | 1995 | 7498847 |
| evaluation of five commercial tests for detection of immunoglobulin m antibodies to human parvovirus b19. | the following commercial tests for detection of immunoglobulin m antibodies to human parvovirus b19 were evaluated: ideia parvovirus b19-igm, mrl diagnostics human parvovirus b19 igm elisa, parvoscan-b19, and biotrin parvo b19 igm eia and if. a total of 203 serum specimens from patients who probably have current b19 infections or have other viral infections and sera with rheumatoid factor were investigated. between 75 and 79 of 102 serum samples from patients thought to have current b19 infectio ... | 1995 | 7615755 |
| cloning and sequencing of the simian parvovirus genome. | we recently reported the identification of a novel simian parvovirus in cynomolgus monkeys with severe anemia. we now describe the cloning and sequencing of 4986 nucleotides of the viral dna. like the human parvovirus b19, simian parvovirus encapsidates both positive and negative single-stranded dna. the positive strand contains two large open reading frames, with the left open reading frame encoding the nonstructural protein(s) and the right reading frame encoding the two capsid proteins. simia ... | 1995 | 7618269 |
| anticomplementary activity in serum samples from patients with acute parvovirus b19 infection. | of 65 serum samples submitted for diagnostic purposes which proved to be anti-complementary by complement fixation test, 49 were parvovirus b19 igm positive. forty four of the 49 serum samples were from patients with arthropathy. acute parvovirus b19 infection should be suspected when a patient has symptoms of disease of the joints and the serum is anticomplementary. | 1995 | 7629301 |
| association between human parvovirus b19 infection and arthritis. | to gain information concerning the association between parvovirus b19 infection and arthritis. | 1995 | 7632094 |
| observational study of effect of intrauterine transfusions on outcome of fetal hydrops after parvovirus b19 infection. | the role of intrauterine transfusion for fetal hydrops arising from maternal paravirus b19 infection is unclear. 66 cases of fetal hydrops arising from b19 infection were reported in england and wales between june, 1992, and september, 1994. in 29 cases the fetus was dead at the time of the first abnormal ultrasound or a therapeutic abortion as performed; 12 of the 38 alive at the first abnormal scan received intrauterine transfusions and 3 of the 12 died. 26 did not receive intrauterine transfu ... | 1995 | 7475774 |
| [parvovirus b19 infection as the cause of hepatitis and neutrophil granulocytosis in a 20-year old woman]. | a case of parvovirus b19 infection (erythema infectiosum) in a 20 year old woman is presented. the patient presented with fever, arthritis in one knee, neutrophil granulocytosis and biochemical evidence of hepatitis. serological evidence of parvovirus b19 infection was found as the only explanation of the clinical picture. hepatitis was due to parvovirus b19 infection as there was no serological evidence of ebv or cmv reactivation. neutrophil granulocytosis and thrombocytosis were found and were ... | 1995 | 7483080 |
| [25-year-old patient with pancytopenia]. | | 1995 | 7499077 |
| adeno-associated virus 2-mediated high efficiency gene transfer into immature and mature subsets of hematopoietic progenitor cells in human umbilical cord blood. | recombinant adeno-associated virus 2 (aav) virions were constructed containing a gene for resistance to neomycin (neor), under the control of either the herpesvirus thymidine kinase (tk) gene promoter (vtk-neo), or the human parvovirus b19 p6 promoter (vb19-neo), as well as those containing an upstream erythroid cell-specific enhancer (hs-2) from the locus control region of the human beta-globin gene cluster (vhs2-tk-neo; vhs2-b19-neo). these recombinant virions were used to infect either low de ... | 1994 | 7515101 |
| subunit interaction in b19 parvovirus empty capsids. | b19 parvovirus is a small single-stranded dna virus with a genome that encodes only two structural proteins, designated vp1 and vp2. 60 copies of the structural proteins assemble into the viral capsid, with approximately 95% vp2 and 5% vp1. recombinant empty capsids composed of vp2 alone or of vp2 and vp1 self-assemble into particles that are morphologically indistinguishable from full virions. empty capsids containing both vp2 and vp1 elicit a strong neutralizing antibody response when used to ... | 1994 | 7516147 |
| age specific antibody prevalence to parvovirus b19: how many women are infected in pregnancy? | infection with parvovirus b19 is an important cause of late fetal mortality in the second trimester, and many infections in pregnancy remain undiagnosed. a serological survey stratified by age has been used to estimate the incidence of maternal infection with parvovirus b19 in pregnancy. serum remaining from specimens submitted for diagnosis from 6864 people of all ages to seven public health laboratories in england was tested for antibody to parvovirus b19. the antibody prevalence rose with age ... | 1994 | 7522807 |
| screening maternal serum alpha-fetoprotein levels and human parvovirus antibodies. | the association between gestational infection with human parvovirus (b19) and fetal loss has increased interest in this virus and demand for diagnostic testing. however, serological assays for b19 are not yet widely available. maternal serum alpha-fetoprotein (msafp) testing is commonly used during the second trimester to screen for various fetal defects. we attempted to determine whether an elevated level of msafp would be an appropriate indication for b19-specific tests. over a 26-month period ... | 1994 | 7524057 |
| peptides derived from the unique region of b19 parvovirus minor capsid protein elicit neutralizing antibodies in rabbits. | b19 parvovirus is pathogenic in humans. the virus propagates in the bone marrow, where it is cytotoxic to erythroid progenitor cells. antibodies appear in blood after infection and neutralize virus in vitro; infection appears to confer lasting immunity. the predominant immune response on immunoblot is to the minor capsid protein (vp1), which differs from the major capsid protein (vp2) by an additional 227 amino acids. we previously demonstrated that antisera directed to a fusion protein containi ... | 1995 | 7530397 |
| design and production of a target-specific monoclonal antibody to parvovirus b19 capsid proteins. | native parvovirus b19 was used as antigen to produce a mouse monoclonal antibody, r92f6, which reacted with b19 vp1 and vp2, neutralised the virus in bone marrow culture, and labelled infected cells in paraffin-embedded tissues from cases of b19-related fetal hydrops. the b19 epitope recognised by r92f6 (amino acids 328-344 from the amino terminal region of b19 vp2) appears to be highly conserved, since these tissue specimens were obtained over a 13 year period from widely spaced locations in th ... | 1995 | 7534801 |
| parvovirus-based vectors for human gene therapy. | it is becoming increasingly clear that the parvovirus-based vectors may prove to be a useful alternative to the more commonly used retroviral vectors in human gene therapy. specifically, the adeno-associated virus 2 (aav), a human parvovirus, has gained particular attention in view of its nonpathogenic nature as well as its remarkable site-specificity of integration into the human chromosome. using the recombinant aav vector system, it is feasible to obtain high-efficiency transduction of slow- ... | 1994 | 7538355 |
| human parvovirus b19 infection: immunohistochemical and electron microscopic studies of skin lesions. | erythema infectiosum is known to be caused by human parvovirus b19 and shows characteristic clinical skin manifestations in children, although adult cases of human parvovirus b19 infection do not always show such characteristic features. recently, we experienced an epidemic adult cases of human parvovirus b19 infection and examined the erythematous skin lesion by immunohistochemistry and electron microscopy to clarify the pathogenesis of the skin manifestations. light microscopic examination sho ... | 1995 | 7560352 |
| parvovirus b19 infection in hospital workers: community or hospital acquisition? | a suspected nosocomial outbreak of parvovirus b19 infection in a maternity ward was investigated in february 1994. questionnaires were administered and sera collected from maternity ward staff (n = 91), other ward staff in the same hospital (n = 101), and maternity ward staff at a nearby hospital (n = 81). blood donors (n = 265) were used as community controls. recent infection (parvovirus b19 igm positivity) in susceptible persons (parvovirus b19 igg-negative or igm-positive) was common among a ... | 1995 | 7561182 |
| [postpartum parvovirus b19-associated acute pure red cell aplasia and hemophagocytic syndrome]. | a 30-year-old postpartum woman was admitted to our hospital because of progressive anemia, malaise, night sweating, headache and low grade fever which began 9 days after delivery (day 0). she had normocytic hypochromic anemia accompanied with marked decrease in reticulocytes. in addition, a temporary decrease in platelets and white blood cells especially neutrophils were observed. bone marrow smears showed an apparent decrease in erythroid cells and the presence of giant proerythroblasts (1.2%) ... | 1995 | 7563595 |