| exposure to selected pathogens in to selected pathogens in geoffroy's cats and domestic carnivores from central argentina. | wild carnivores share a high percentage of parasites and viruses with closely related domestic carnivores. because of increased overlap and potential contact with domestic species, we conducted a retrospective serosurvey for 11 common carnivore pathogens in 40 geoffroy's cats (leopardus geoffroyi) sampled between 2000 and 2008 within or near two protected areas in central argentina (lihué calel national park, la pampa, and campos del tuyú national park, buenos aires), as well as five domestic ca ... | 2012 | 23060491 |
| prevalence and risk factors for viral exposure in rural dogs around protected areas of the atlantic forest. | despite the crucial role of domestic dogs as reservoirs for zoonosis and some of the most threatening diseases for wild carnivores such as distemper and parvovirosis, little is known about the epidemiological features and the risk factors involved in pathogen exposure of dogs that live in human/wildlife interfaces and actually contacts wildlife. through a cross-sectional serological approach and questionnaire survey, we assessed the prevalence along with individual and environment-associated ris ... | 2016 | 26822375 |
| establishment of reverse transcription loop-mediated isothermal amplification for rapid detection and differentiation of canine distemper virus infected and vaccinated animals. | although widespread vaccination against canine distemper virus (cdv) has been conducted for many decades, several canine distemper outbreaks in vaccinated animals have been reported frequently. in order to detect and differentiate the wild-type and vaccine strains of the cdv from the vaccinated animals, a novel reverse transcription loop-mediated isothermal amplification (rt-lamp) method was developed. a set of four primers-two internal and two external-were designed to target the h gene for the ... | 2015 | 25769803 |
| canine adenovirus type 1 in a fennec fox (vulpes zerda). | a 10-mo-old female fennec fox (vulpes zerda) with drooling suddenly died and was examined postmortem. histologic examination of different tissue samples was performed. vacuolar degeneration and diffuse fatty change were observed in the liver. several diagnostic methods were used to screen for canine parvovirus, canine distemper virus, canine influenza virus, canine coronavirus, canine parainfluenza virus, and canine adenovirus (cadv). only cadv type 1 (cadv-1) was detected in several organs (liv ... | 2014 | 25632689 |
| antiviral activity of a bacillus sp. p34 peptide against pathogenic viruses of domestic animals. | p34 is an antimicrobial peptide produced by a bacillus sp. strain isolated from the intestinal contents of a fish in the brazilian amazon basin with reported antibacterial activity. the aim of this work was to evaluate the peptide p34 for its in vitro antiviral properties against canine adenovirus type 2 (cav-2), canine coronavirus (ccov), canine distemper virus (cdv), canine parvovirus type 2 (cpv-2), equine arteritis virus (eav), equine influenza virus (eiv), feline calicivirus (fcv) and felin ... | 2014 | 25477947 |
| pathogens of wild maned wolves (chrysocyon brachyurus) in brazil. | the maned wolf, chrysocyon brachyurus, is an endangered neotropical canid that survives at low population densities. diseases are a potential threat for its conservation but to date have been poorly studied. we performed clinical evaluations and investigated the presence of infectious diseases through serology and coprologic tests on maned wolves from galheiro natural private reserve, perdizes city, minas gerais state, southeastern brazil. fifteen wolves were captured between 2003 and 2008. we f ... | 2012 | 23060508 |
| polymerase spiral reaction (psr): a novel, visual isothermal amplification method for detection of canine parvovirus 2 genomic dna. | canine parvovirus-2 (cpv-2), which is ubiquitously distributed worldwide, causes severe and often fatal gastroenteritis in dogs. accurate, differential and rapid diagnosis of canine parvoviral enteritis remains a challenge for clinicians. a recently developed isothermal amplification technique, polymerase spiral reaction (psr), was optimized for the first time for a viral pathogen with reference recombinant plasmid standards from different cpv-2 antigenic variants (cpv-2, cpv-2a, cpv-2b and cpv- ... | 2017 | 28349355 |
| the occurrence of pathogens in an endangered population of american badgers (taxidea taxus jacksoni) in ontario, canada. | american badgers ( taxidea taxus jacksoni) at the periphery of the species' range in ontario, canada, are listed as endangered because of an estimated population size of <200 mature individuals. the main threats faced by this population include habitat loss and road mortality. however, on 18 november 2013, a radio-implanted badger was found nonresponsive in an agricultural field with signs consistent with canine distemper virus infection, which was subsequently confirmed. this prompted our inves ... | 2017 | 27749192 |
| age-related presence of selected viral and bacterial pathogens in paraffin-embedded lung samples of dogs with pneumonia. | the aim of this retrospective study was to detect selected pathogens in pneumonic lung tissue of dogs of different age groups by immunohistochemistry (ihc), in situ hybridisation (ish) or polymerase chain reaction (pcr) in order to get information about their involvement in pneumonia formation. in archived formalin-fixed and paraffin wax-embedded lung samples from 68 cases with the clinical and histologic diagnosis of pneumonia the histological pattern of pneumonia was re-evaluated and the sampl ... | 2016 | 26919147 |
| dog overpopulation and burden of exposure to canine distemper virus and other pathogens on santa cruz island, galapagos. | dog overpopulation and diseases are hazards to native island species and humans on the galapagos. vaccination and importation of dogs are prohibited on the galapagos. risk management of these hazards requires the use of science-based risk assessment and risk communication. the objectives of the study reported here were (i) to estimate the human:dog ratio and (ii) the prevalence of and identify exposure factors associated with positive antibody titers to canine distemper virus (cdv) and other pat ... | 2016 | 26700426 |
| serological detection of infection with canine distemper virus, canine parvovirus and canine adenovirus in communal dogs from zimbabwe. | domestic dogs are common amongst communities in sub-saharan africa and may serve as important reservoirs for infectious agents that may cause diseases in wildlife. two agents of concern are canine parvovirus (cpv) and canine distemper virus (cdv), which may infect and cause disease in large carnivore species such as african wild dogs and african lions, respectively. the impact of domestic dogs and their diseases on wildlife conservation is increasing in zimbabwe, necessitating thorough assessmen ... | 2014 | 25686382 |
| serologic evidence for selected infectious diseases in marsican brown bears (ursus arctos marsicanus) in italy (2004-09). | we tested 30 serum samples collected during 2004-09 from 22 free-ranging marsican brown bears (ursus arctos marsicanus) in the national park of abruzzo, lazio, and molise, italy, for antibodies against canine distemper virus (cdv), canine adenovirus type 2 (cav-2), canine parvovirus type 2 (cpv-2), brucella spp., and eight leptospira interrogans sensu lato serovars. antibody to cdv was detected in 11 samples (37%); only two bears (10%) had detectable cav-2 and brucella spp. antibodies; three bea ... | 2015 | 25375945 |
| duration of immunity in red wolves (canis rufus) following vaccination with a modified live parvovirus and canine distemper vaccine. | there is growing information available regarding duration of immunity for core vaccines in both domestic and nondomestic species. vaccination protocols in nondomestic canids have frequently followed guidelines developed for the domestic dog; however, these protocols can be inappropriate for nondomestic canids such as the african wild dog (lycaon pictus), leaving some animals susceptible to infectious disease and others at risk for contracting vaccine-induced disease. in this study, red wolves (c ... | 2014 | 25314821 |
| epidemiology of viral pathogens of free-ranging dogs and indian foxes in a human-dominated landscape in central india. | there is an increasing concern that free-ranging domestic dog (canis familiaris) populations may serve as reservoirs of pathogens which may be transmitted to wildlife. we documented the prevalence of antibodies to three viral pathogens, canine parvovirus (cpv), canine distemper virus (cdv) and canine adenovirus (cav), in free-ranging dog and sympatric indian fox (vulpes bengalensis) populations in and around the great indian bustard wildlife sanctuary, in maharashtra, central india. a total of 2 ... | 2014 | 25135467 |
| parasitology, virology, and serology of free-ranging coyotes (canis latrans) from central georgia, usa. | we examined 31 free-ranging coyotes (canis latrans) from central georgia, usa, for select parasites and viral agents. sixteen coyotes had adult heartworms (dirofilaria immitis). serum samples from 27 animals revealed antibodies against canine parvovirus (100%), canine distemper virus (48%), canine adenovirus (37%), and trypanosoma cruzi (7%); none were detected against leishmania spp. twenty-two of 24 (92%) coyotes were positive for toxoplasma gondii. real-time pcr of feces revealed 32% of coyot ... | 2014 | 25098300 |
| canine kobuvirus infections in korean dogs. | to investigate canine kobuvirus (cakov) infection, fecal samples (n = 59) were collected from dogs with or without diarrhea (n = 21 and 38, respectively) in the republic of korea (rok) in 2012. cakov infection was detected in four diarrheic samples (19.0 %) and five non-diarrheic samples (13.2 %). all cakov-positive dogs with diarrhea were found to be infected in mixed infections with canine distemper virus and canine parvovirus or canine adenovirus. there was no significant difference in the pr ... | 2014 | 24906525 |
| high prevalence of antibodies against canine adenovirus (cav) type 2 in domestic dog populations in south africa precludes the use of cav-based recombinant rabies vaccines. | rabies in dogs can be controlled through mass vaccination. oral vaccination of domestic dogs would be useful in the developing world, where greater vaccination coverage is needed especially in inaccessible areas or places with large numbers of free-roaming dogs. from this perspective, recent research has focused on development of new recombinant vaccines that can be administered orally in a bait to be used as adjunct for parenteral vaccination. one such candidate, a recombinant canine adenovirus ... | 2013 | 23867013 |
| assessing demographic and epidemiologic parameters of rural dog populations in india during mass vaccination campaigns. | mass vaccination of dogs is a mainstay for efforts to control rabies and other viral pathogens. the success of such programs is a function of the ability to vaccinate sufficient proportions of animals to develop herd immunity. however, fully assessing success in reaching target vaccination-levels and in understanding the outcome of mass vaccination efforts is hindered if insufficient information is available on the demographics of dog populations and the prevalence of the targeted pathogens. whi ... | 2013 | 23664490 |
| concomitant canine distemper, infectious canine hepatitis, canine parvoviral enteritis, canine infectious tracheobronchitis, and toxoplasmosis in a puppy. | the concomitant infections of canine distemper virus (cdv), canine adenovirus a types 1 (cadv-1) and 2 (cadv-2), canine parvovirus type 2 (cpv-2), and toxoplasma gondii are described in a 43-day-old mixed-breed puppy. clinically, there were convulsions and blindness with spontaneous death; 14 siblings of this puppy, born to a 10-month-old dam, which was seropositive (titer: 1,024) for t. gondii, also died. necropsy revealed unilateral corneal edema (blue eye), depletion of intestinal lymphoid ti ... | 2013 | 23293164 |
| duration of serological response to canine parvovirus-type 2, canine distemper virus, canine adenovirus type 1 and canine parainfluenza virus in client-owned dogs in australia. | to determine whether client-owned dogs in australia, last vaccinated with canvac(®) vaccines containing canine parvovirus-type 2 (cpv-2), canine distemper virus (cdv), canine adenovirus type 2 (cav-2) ± canine parainfluenza virus (cpiv) at least 18 months ago, were seropositive or responded serologically to revaccination. | 2012 | 23186088 |
| booster effect of canine distemper, canine parvovirus infection and infectious canine hepatitis combination vaccine in domesticated adult dogs. | domesticated adult dogs with antibody titer classified as below 'high' to one or more of canine distemper virus (cdv), canine parvovirus type-2 (cpv-2) and canine adenovirus type-1 (cadv-1) were then given an additional inoculation, and the effectiveness of this booster evaluated 2 months later. consequently, cdv and cadv-1 antibody titer experienced a significant increase, but the same effect was not observed in the antibody titer of cpv-2. these findings suggest that with additional inoculatio ... | 2012 | 22672137 |
| bidirectional control of anxiety-related behaviors in mice: role of inputs arising from the ventral hippocampus to the lateral septum and medial prefrontal cortex. | anxiety is an adaptive response to potentially threatening situations. exaggerated and uncontrolled anxiety responses become maladaptive and lead to anxiety disorders. anxiety is shaped by a network of forebrain structures, including the hippocampus, septum, and prefrontal cortex. in particular, neural inputs arising from the ventral hippocampus (vhpc) to the lateral septum (ls) and medial prefrontal cortex (mpfc) are thought to serve as principal components of the anxiety circuit. however, the ... | 2017 | 28294135 |
| excitatory hindbrain-forebrain communication is required for cisplatin-induced anorexia and weight loss. | cisplatin chemotherapy is commonly used to treat cancer despite severe energy balance side effects. in rats, cisplatin activates nucleus tractus solitarius (nts) projections to the lateral parabrachial nucleus (lpbn) and calcitonin-gene related peptide (cgrp) projections from the lpbn to the central nucleus of the amygdala (cea). we demonstrated previously that cea glutamate receptor signaling mediates cisplatin-induced anorexia and body weight loss. here, we used neuroanatomical tracing, immuno ... | 2017 | 28077715 |
| evolution and cryo-electron microscopy capsid structure of a north american bat adenovirus and its relationship to other mastadenoviruses. | since the first description of adenoviruses in bats in 2006, a number of micro- and megabat species in europe, africa, and asia have been shown to carry a wide diversity of adenoviruses. here, we report on the evolutionary, biological, and structural characterization of a novel bat adenovirus (btadv) recovered from a rafinesque's big-eared bat (corynorhinus rafinesquii) in kentucky, usa, which is the first adenovirus isolated from north american bats. this virus (btadv 250-a) exhibits a close ph ... | 2017 | 27807242 |
| serological and molecular epidemiology of canine adenovirus type 1 in red foxes (vulpes vulpes) in the united kingdom. | canine adenovirus type 1 (cav-1) causes infectious canine hepatitis (ich), a frequently fatal disease which primarily affects canids. in this study, serology (elisa) and molecular techniques (pcr/qpcr) were utilised to investigate the exposure of free-ranging red foxes (vulpes vulpes) to cav-1 in the united kingdom (uk) and to examine their role as a wildlife reservoir of infection for susceptible species. the role of canine adenovirus type 2 (cav-2), primarily a respiratory pathogen, was also e ... | 2016 | 27796367 |
| recombinant canine adenovirus type-2 expressing tgrop16 provides partial protection against acute toxoplasma gondii infection in mice. | we previously demonstrated that the survival time of balb/c mice challenged with toxoplasma gondii rh strain was prolonged by immunising the mice with a eukaryotic vector expressing the protein rop16 of t. gondii. building upon previous findings, we are exploring improved vaccination strategies to enhance protection. in this work, a novel recombinant canine adenovirus type 2 expressing rop16 (cav-2-rop16) of t. gondii was constructed and identified to express rop16 in madin-darby canine kidney c ... | 2016 | 27742446 |
| a duplex real-time pcr assay based on taqman technology for simultaneous detection and differentiation of canine adenovirus types 1 and 2. | canine adenoviruses are a major cause of disease in dogs, coyotes, red foxes and wolves, as well as in other carnivores and marine mammals. canine adenovirus type 1 (cadv-1) and canine adenovirus type 2 (cadv-2) cause infectious canine hepatitis (ich) and infectious tracheobronchitis (itb), respectively. in this study, a duplex real-time pcr assay for simultaneous detection and characterisation of cadv-1 and cadv-2 was developed by using a single primer pair and virus-specific probes. the assay ... | 2016 | 27040113 |
| canine adenovirus type 1 and pasteurella pneumotropica co‑infection in a puppy. | in 2008, a 2 months-old male german shepherd was presented with fever, depression, and evident organic wasting. the puppy died within 48 hours after the onset of clinical signs. a complete necropsy was performed. bacteriological examination of samples from the brain, lung, liver, spleen, and bone marrow tested positive for pasteurella pneumotropica. histopathology demonstrated inflammatory and vascular lesions in the central nervous system and internal organs. canine adenovirus type 1 nucleic ac ... | 2017 | 27033531 |
| metabolic flux profiling of mdck cells during growth and canine adenovirus vector production. | canine adenovirus vector type 2 (cav2) represents an alternative to human adenovirus vectors for certain gene therapy applications, particularly neurodegenerative diseases. however, more efficient production processes, assisted by a greater understanding of the effect of infection on producer cells, are required. combining [1,2-(13)c]glucose and [u-(13)c]glutamine, we apply for the first time (13)c-metabolic flux analysis ((13)c-mfa) to study e1-transformed madin-darby canine kidney (mdck) cells ... | 2016 | 27004747 |
| infectious canine hepatitis in red foxes (vulpes vulpes) in wildlife rescue centres in the uk. | outbreaks of infectious canine hepatitis are described in red foxes ( italic! vulpes vulpes) at two wildlife rescue centres in the uk. disease occurred in two-month-old to four-month-old juvenile foxes, which were held in small enclosures in groups of three to eight animals. the foxes died or were euthanased after a short clinical course, sometimes including neurological signs and jaundice, with a high case fatality rate. four red foxes submitted for postmortem examination had enlarged, congeste ... | 2016 | 27001767 |
| cav-2--why a canine virus is a neurobiologist's best friend. | canine adenovirus type 2 (cav-2) vectors are powerful tools for fundamental and applied neurobiology due to their negligible immunogenicity, preferential transduction of neurons, widespread distribution via axonal transport, and duration of expression in the mammalian brain. cav-2 vectors are internalized in neurons by the selective use of coxsackievirus and adenovirus receptor (car), which is located at the presynapse in neurons. neuronal internalization and axonal transport is mediated by car, ... | 2015 | 26298516 |
| transcriptional response of human neurospheres to helper-dependent cav-2 vectors involves the modulation of dna damage response, microtubule and centromere gene groups. | brain gene transfer using viral vectors will likely become a therapeutic option for several disorders. helper-dependent (hd) canine adenovirus type 2 vectors (cav-2) are well suited for this goal. these vectors are poorly immunogenic, efficiently transduce neurons, are retrogradely transported to afferent structures in the brain and lead to long-term transgene expression. cav-2 vectors are being exploited to unravel behavior, cognition, neural networks, axonal transport and therapy for orphan di ... | 2015 | 26207738 |
| evaluation of helper-dependent canine adenovirus vectors in a 3d human cns model. | gene therapy is a promising approach with enormous potential for treatment of neurodegenerative disorders. viral vectors derived from canine adenovirus type 2 (cav-2) present attractive features for gene delivery strategies in the human brain, by preferentially transducing neurons, are capable of efficient axonal transport to afferent brain structures, have a 30-kb cloning capacity and have low innate and induced immunogenicity in preclinical tests. for clinical translation, in-depth preclinical ... | 2016 | 26181626 |
| the intracellular domain of the coxsackievirus and adenovirus receptor differentially influences adenovirus entry. | the coxsackievirus and adenovirus receptor (car) is a cell adhesion molecule used as a docking molecule by some adenoviruses (advs) and group b coxsackieviruses. we previously proposed that the preferential transduction of neurons by canine adenovirus type 2 (cav-2) is due to car-mediated internalization. our proposed pathway of cav-2 entry is in contrast to that of human adv type 5 (hadv-c5) in nonneuronal cells, where internalization is mediated by auxiliary receptors such as integrins. we the ... | 2015 | 26136571 |
| development of a sybr green real-time pcr assay with melting curve analysis for simultaneous detection and differentiation of canine adenovirus type 1 and type 2. | canine adenovirus type 1 (cadv-1) and canine adenovirus type 2 (cadv-2) cause infectious canine hepatitis (ich) and infectious tracheobronchitis (itb) in dogs, respectively. cases of ich have been documented in recent years and recent surveys have demonstrated a wide percentage of asymptomatic cadv-1 infection in the canine population. since both cadv types are detectable in the same biological matrices, and viral coinfection with cadv-1 and cadv-2 are reported with high frequency, it is urgent ... | 2015 | 26028428 |
| experimental oral immunization of ferret badgers (melogale moschata) with a recombinant canine adenovirus vaccine cav-2-e3δ-rgp and an attenuated rabies virus srv9. | ferret badgers (melogale moschata) are a major reservoir of rabies virus in southeastern china. oral immunization has been shown to be a practical method for wildlife rabies management in europe and north america. two groups of 20 ferret badgers were given a single oral dose of a recombinant canine adenovirus-rabies vaccine, cav-2-e3δ-rgp, or an experimental attenuated rabies virus vaccine, srv9. at 21 days, all ferret badgers had seroconverted, with serum virus-neutralizing antibodies ranging f ... | 2014 | 24506428 |
| a prb-responsive, rgd-modified, and hyaluronidase-armed canine oncolytic adenovirus for application in veterinary oncology. | human and canine cancer share similarities such as genetic and molecular aspects, biological complexity, tumor epidemiology, and targeted therapeutic treatment. lack of good animal models for human adenovirotherapy has spurred the use of canine adenovirus 2-based oncolytic viruses. we have constructed a canine oncolytic virus that mimics the characteristics of our previously published human adenovirus icovir17: expression of e1a controlled by e2f sites, deletion of the prb-binding site of e1a, i ... | 2014 | 24448161 |
| hemorrhagic and necrotizing hepatitis associated with administration of a modified live canine adenovirus-2 vaccine in a maned wolf (chrysocyon brachyurus). | a 15-yr-old, female, maned wolf (chrysocyon brachyurus) was euthanized after presenting semicomatose with severe, uncontrolled frank hemorrhage from her rectum 6 days following a routine physical examination and vaccination. histopathology indicated severe hemorrhagic and necrotizing hepatitis with intranuclear basophilic inclusion bodies in the liver that were thought to be consistent with adenoviral infection. further classification by polymerase chain reaction, immunohistochemical staining, v ... | 2012 | 22779244 |
| chromatography purification of canine adenoviral vectors. | canine adenovirus vectors (cav2) are currently being evaluated for gene therapy, oncolytic virotherapy, and as vectors for recombinant vaccines. despite the need for increasing volumes of purified cav2 preparations for preclinical and clinical testing, their purification still relies on the use of conventional, scale-limited cscl ultracentrifugation techniques. a complete downstream processing strategy for cav2 vectors based on membrane filtration and chromatography is reported here. microfiltra ... | 2012 | 22799886 |
| molecular epidemiology of canine adenovirus type 1 and type 2 in free-ranging red foxes (vulpes vulpes) in italy. | to date, no studies exist regarding the presence of canine adenovirus (cadv) infection in foxes in italy. furthermore, the majority of worldwide investigations regarding the presence of cadv in foxes have been carried out using common serological assays which are unable to differentiate between cadv type 1 and cadv type 2. to assess the presence of viral infection in italian red foxes (vulpes vulpes), thirty-two subjects shot during the regular hunting season in the province of pisa (tuscany, it ... | 2013 | 23201241 |
| molecular analysis of a fragment of gene e1b 19k of canine adenovirus 2 (cav-2) isolated from dogs with symptoms of cough. | the aim of this study was to perform molecular analysis of canine adenovirus 2 (cav-2) e1b 19k gene fragment isolated from 20 dogs of various breeds (12 males and 8 females aged 1-9 years), with clinical symptoms of upper respiratory tract infections, from the lubelszczyzna region. nasal swabs were taken from dogs. dna of cav-2 was detected using the pcr method in 16 swabs. all pcr products were sequenced, and the obtained sequences were compared with each other and with the sequence of the e1b ... | 2012 | 23214360 |
| a siglec-like sialic-acid-binding motif revealed in an adenovirus capsid protein. | sialic-acid-binding immunoglobulin-like lectins (siglecs) are a family of transmembrane receptors that are well documented to play roles in regulation of innate and adaptive immune responses. to see whether the features that define the molecular recognition of sialic acid were found in other sialic-acid-binding proteins, we analyzed 127 structures with bound sialic acids found in the protein data bank database. of these, the canine adenovirus 2-fiber knob protein showed close local structural re ... | 2012 | 22522600 |
| 3d cultures of human neural progenitor cells: dopaminergic differentiation and genetic modification. [corrected]. | central nervous system (cns) disorders remain a formidable challenge for the development of efficient therapies. cell and gene therapy approaches are promising alternatives that can have a tremendous impact by treating the causes of the disease rather than the symptoms, providing specific targeting and prolonged duration of action. hampering translation of gene-based therapeutic treatments of neurodegenerative diseases from experimental to clinical gene therapy is the lack of valid and reliable ... | 2012 | 22433395 |
| a serosurvey of diseases of free-ranging gray wolves (canis lupus) in minnesota. | we tested serum samples from 387 free-ranging wolves ( canis lupus ) from 2007 to 2013 for exposure to eight canid pathogens to establish baseline data on disease prevalence and spatial distribution in minnesota's wolf population. we found high exposure to canine adenoviruses 1 and 2 (88% adults, 45% pups), canine parvovirus (82% adults, 24% pups), and lyme disease (76% adults, 39% pups). sixty-six percent of adults and 36% of pups exhibited exposure to the protozoan parasite neospora caninum . ... | 2017 | 28192048 |
| transducing properties of a pre-structured α-helical dpt-peptide containing a short canine adenovirus type 2 e4orf4 pp2a1-binding sequence. | induction of the death pathway resulting from the specific interaction of the pp2a1 phosphatase with adenoviral e4orf4 protein is a promising approach for cancer therapy. with the aim of deregulating tumor pathways, and mimicking e4orf4 anti-cancer signal, we have previously proposed the dpt technology concept, based on design of specific pp1/pp2a interacting penetrating peptides. | 2013 | 23500018 |
| pathological findings in the red fox (vulpes vulpes), stone marten (martes foina) and raccoon dog (nyctereutes procyonoides), with special emphasis on infectious and zoonotic agents in northern germany. | anthropogenic landscape changes contributed to the reduction of availability of habitats to wild animals. hence, the presence of wild terrestrial carnivores in urban and peri-urban sites has increased considerably over the years implying an increased risk of interspecies spillover of infectious diseases and the transmission of zoonoses. the present study provides a detailed characterisation of the health status of the red fox (vulpes vulpes), stone marten (martes foina) and raccoon dog (nyctereu ... | 2017 | 28399176 |
| serology and protein electrophoresis for evidence of exposure to 12 mink pathogens in free-ranging american mink (neovison vison) in argentina. | basic pathologic characteristics for farmed minks were previously reported worldwide. however, its status in the wild has not been studied in detail. | 2017 | 28635524 |
| impact of e1 and cre on adenovirus vector amplification: developing mdck cav-2-e1 and e1-cre transcomplementing cell lines. | adenovirus vectors have been extensively studied through the manipulation of viral genome. however, little attention is being paid to their producer cell-lines; cells are selected according to virus yields, neglecting the expression profile of transcomplementing gene products underlying cell performance. this work evaluates the impact of e1 (e1a and e1b) and cre recombinase levels in the production of e1-deleted and helper-dependent canine adenovirus type 2 (cav-2) vectors using mdck cells. e1a ... | 2013 | 23565229 |
| surveillance for antibodies against six canine viruses in wild raccoons (procyon lotor) in japan. | raccoons (procyon lotor) are found worldwide. they are frequently seen in crowded inner cities as well as in forests or wooded areas, often living in proximity to humans and their pets. we examined sera from 100 wild raccoons in japan for antibodies to six canine viruses with veterinary significance to assess their potential as reservoirs. we also aimed to understand the distribution of potentially infected wildlife. we found that 7% of samples were seropositive for canine distemper virus (cdv), ... | 2017 | 28715293 |
| combined antiapoptotic and antioxidant approach to acute neuroprotection for stroke in hypertensive rats. | we hypothesized that targeting key points in the ischemic cascade with combined neuroglobin (ngb) overexpression and c-jun n-terminal kinase (jnk) inhibition (sp600125) would offer greater neuroprotection than single treatment after in vitro hypoxia/reoxygenation and in a randomized, blinded in vivo experimental stroke study using a clinically relevant rat strain. male spontaneously hypertensive stroke-prone rats underwent transient middle cerebral artery occlusion (tmcao) and were divided into ... | 2013 | 23632970 |
| differentiated neuroprogenitor cells incubated with human or canine adenovirus, or lentiviral vectors have distinct transcriptome profiles. | several studies have demonstrated the potential for vector-mediated gene transfer to the brain. helper-dependent (hd) human (had) and canine (cav-2) adenovirus, and vsv-g-pseudotyped self-inactivating hiv-1 vectors (lv) effectively transduce human brain cells and their toxicity has been partly analysed. however, their effect on the brain homeostasis is far from fully defined, especially because of the complexity of the central nervous system (cns). with the goal of dissecting the toxicogenomic s ... | 2013 | 23922808 |
| canine adenovirus type 2 vector generation via i-sce1-mediated intracellular genome release. | when canine adenovirus type 2 (cadv-2, or also commonly referred to as cav-2) vectors are injected into the brain parenchyma they preferentially transduce neurons, are capable of efficient axonal transport to afferent regions, and allow transgene expression for at last >1 yr. yet, translating these data into a user-friendly vector platform has been limited because cav-2 vector generation is challenging. generation of e1-deleted adenovirus vectors often requires transfection of linear dna fragmen ... | 2013 | 23936483 |
| defining functional gene-circuit interfaces in the mouse nervous system. | complexity in the nervous system is established by developmental genetic programs, maintained by differential genetic profiles and sculpted by experiential and environmental influence over gene expression. determining how specific genes define neuronal phenotypes, shape circuit connectivity and regulate circuit function is essential for understanding how the brain processes information, directs behavior and adapts to changing environments. mouse genetics has contributed greatly to current percep ... | 2014 | 24007626 |
| novel mastadenovirus infection and clinical disease in a pygmy marmoset (callithrix [cebuella] pygmaea). | we describe the detection and successful isolation of a novel mastadenovirus from a pygmy marmoset (callithrix [cebuella] pygmaea) that died following an episode of severe respiratory signs. pathologic/histopathologic examination revealed hydrothorax and catarrhal bronchopneumonia with pronounced desquamation of the bronchiolar epithelial cells, while in other airways a marked hyperplasia of the epithelial lining and numerous giant cells could be observed. we obtained partial sequence data from ... | 2013 | 24012169 |
| study of adenovirus and car axonal transport in primary neurons. | vectors derived from the canine adenovirus serotype 2 (cav-2) possess a high neurotropism and efficient retrograde transport that lead to widespread neuronal transduction in the central nervous system (cns) of various animals. these abilities are due to the engagement of virions to the coxsackievirus and adenovirus receptor at the surface of neurons, which is linked to the endocytic and axonal transport machineries. the trafficking of cav-2 and the coxsackievirus and adenovirus receptor (car) ca ... | 2014 | 24132478 |
| canine adenovirus downstream processing protocol. | adenovirus vectors are efficient gene delivery tools. a major caveat with vectors derived from common human adenovirus serotypes is that most adults are likely to have been exposed to the wild-type virus and exhibit active immunity against the vectors. this preexisting immunity limits their clinical success. strategies to circumvent this problem include the use of nonhuman adenovirus vectors. vectors derived from canine adenovirus type 2 (cav-2) are among the best-studied representatives. cav-2 ... | 2014 | 24132487 |
| the serological and virological investigation of canine adenovirus infection on the dogs. | two types of canine adenovirus (cavs), canine adenovirus type 1 (cav-1), the virus which causes infectious canine hepatitis, and canine adenovirus type 2 (cav-2), which causes canine infectious laryngotracheitis, have been found in dogs. in this study, blood samples taken from 111 dogs, which were admitted to the internal medicine clinic of selcuk university, faculty of veterinary medicine, with clinical symptoms. seventy-seven dogs were sampled from isparta and burdur dog shelters by random sam ... | 2013 | 24223508 |
| production and purification of non replicative canine adenovirus type 2 derived vectors. | adenovirus (ad) derived vectors have been widely used for short or long-term gene transfer, both for gene therapy and vaccine applications. because of the frequent pre-existing immunity against the classically used human adenovirus type 5, canine adenovirus type 2 (cav2) has been proposed as an alternative vector for human gene transfer. the well-characterized biology of cav2, together with its ease of genetic manipulation, offer major advantages, notably for gene transfer into the central nervo ... | 2013 | 24326926 |
| corrective gusb transfer to the canine mucopolysaccharidosis vii brain. | severe deficiency in lysosomal β-glucuronidase (β-glu) enzymatic activity results in mucopolysaccharidosis (mps) vii, an orphan disease with symptoms often appearing in early childhood. symptoms are variable, but many patients have multiple organ disorders including neurological defects. at the cellular level, deficiency in β-glu activity leads to abnormal accumulation of glycosaminoglycans (gags), and secondary accumulation of gm2 and gm3 gangliosides, which have been linked to neuroinflammatio ... | 2014 | 24343103 |
| serologic survey of selected viral pathogens in free-ranging eurasian brown bears ( ursus arctos arctos) from slovakia. | we tested sera of 24 free-ranging european brown bears ( ursus arctos) from six regions of slovakia for antibodies to 10 viral agents. we tested sera by an indirect fluorescence antibody test for antibodies to canine distemper virus (cdv), canine coronavirus (ccv), canine parvovirus type 2 (cpv-2), canine adenovirus, canine parainfluenza virus type 2 (cpiv-2), and canine herpesvirus type 1 (chv-1). we used an enzyme-linked immunosorbent assay for detection of antibodies to hepatitis e virus, blu ... | 2019 | 30289326 |
| cav-2-mediated gfp and lrrk2g2019s expression in the macaca fascicularis brain. | parkinson's disease is characterized by motor and nonmotor symptoms that gradually appear as a consequence of the selective loss of dopaminergic neurons in the substantia nigra pars compacta. currently, no treatment can slow parkinson's disease progression. inasmuch, there is a need to develop animal models that can be used to understand the pathophysiological mechanisms underlying dopaminergic neuron death. the initial goal of this study was to determine if canine adenovirus type 2 (cav-2) vect ... | 2020 | 32269512 |
| corrective gusb transfer to the canine mucopolysaccharidosis vii cornea using a helper-dependent canine adenovirus vector. | corneal transparency is maintained, in part, by specialized fibroblasts called keratocytes, which reside in the fibrous lamellae of the stroma. corneal clouding, a condition that impairs visual acuity, is associated with numerous diseases, including mucopolysaccharidosis (mps) type vii. mps vii is due to deficiency in β-glucuronidase (β-glu) enzymatic activity, which leads to accumulation of glycosaminoglycans (gags), and secondary accumulation of gangliosides. here, we tested the efficacy of ca ... | 2014 | 24607662 |
| exposure of alaska brown bears (ursus arctos) to bacterial, viral, and parasitic agents varies spatiotemporally and may be influenced by age. | we collected blood and serum from 155 brown bears (ursus arctos) inhabiting five locations in alaska, us during 2013-16 and tested samples for evidence of prior exposure to a suite of bacterial, viral, and parasitic agents. antibody seroprevalence among alaska brown bears was estimated to be 15% for brucella spp., 10% for francisella tularensis, 7% for leptospira spp., 18% for canine adenovirus type 1 (cav-1), 5% for canine distemper virus (cdv), 5% for canine parvovirus, 5% for influenza a viru ... | 2019 | 30557123 |
| combined use of the canine adenovirus-2 and dreadd-technology to activate specific neural pathways in vivo. | we here describe a technique to transiently activate specific neural pathways in vivo. it comprises the combined use of a cre-recombinase expressing canine adenovirus-2 (cav-2) and an adeno-associated virus (aav-hsyn-dio-hm3d(gq)-mcherry) that contains the floxed inverted sequence of the designer receptor exclusively activated by designer drugs (dreadd) hm3d(gq)-mcherry. cav-2 retrogradely infects projection neurons, which allowed us to specifically express hm3d(gq)-mcherry in neurons that proje ... | 2014 | 24736748 |
| genetic characterization of canine adenovirus type 1 detected by real-time polymerase chain reaction in an oral sample of an italian wolf (canis lupus). | we report the detection of canine adenovirus type 1 dna by real-time pcr technique in an oral sample of an italian wolf (canis lupus italicus). genetic characterization of the virus revealed a strict relationship with viruses detected in dogs (canis lupus familiaris), wolves, and red foxes (vulpes vulpes), suggesting that transmission between wild animals and dogs had occurred. | 2019 | 30789782 |
| impact of adenovirus life cycle progression on the generation of canine helper-dependent vectors. | helper-dependent adenovirus vectors (hdvs) are safe and efficient tools for gene transfer with high cloning capacity. however, the multiple amplification steps needed to produce hdvs hamper a robust production process and in turn the availability of high-quality vectors. to understand the factors behind the low productivity, we analyzed the progression of hdv life cycle. canine adenovirus (ad) type 2 vectors, holding attractive features to overcome immunogenic concerns and treat neurobiological ... | 2015 | 25338917 |
| a simplified system for generating recombinant e3-deleted canine adenovirus-2. | canine adenovirus type 2 (cav-2) has been used extensively as a vector for studying gene therapy and vaccine applications. we describe a simple strategy for generating a replication-competent recombinant cav-2 using a backbone vector and a shuttle vector. the backbone plasmid containing the full-length cav-2 genome was constructed by homologous recombination in escherichia coli strain bj5183. the shuttle plasmid, which has a deletion of 1478 bp in the nonessential e3 viral genome region, was gen ... | 2015 | 25450764 |
| investigation of the presence of canine adenovirus (cadv) in owned dogs in northern italy. | the use of a modified live canine adenovirus (cadv) vaccine has greatly reduced the incidence of infectious canine hepatitis (ich) in dogs. nevertheless, cases of cadv type 1 and 2 (cadv-1 and cadv-2) infection have been recently reported posing questions about the epidemiological situation of cadv in dogs. in order to assess the presence of cadv, samples from 51 dogs presented at a veterinary teaching hospital in bologna, italy, for reasons unrelated with cadv infection, were tested with a poly ... | 2014 | 25468801 |
| canine helper-dependent vectors production: implications of cre activity and co-infection on adenovirus propagation. | the importance of cre recombinase to minimize helper vector (hv) contamination during helper-dependent adenovirus vectors (hdvs) production is well documented. however, cre recombinase, by inducing dna double-strand breaks (dsbs), can cause a reduced proliferation and genotoxic effects in cultured cells. in this work, cre-expressing cell stability, co-infection and their relation to adenovirus amplification/hv contamination were evaluated to develop a production protocol for hd canine adenovirus ... | 2015 | 25774853 |
| protective efficacy of recombinant canine adenovirus type-2 expressing tgrop18 (cav-2-rop18) against acute and chronic toxoplasma gondii infection in mice. | the use of recombinant viral vectors expressing t. gondii antigens is a safe and efficient approach to induce immune responses against the parasite, as well as a valuable tool for vaccine development. we have previously prolonged the survival time of mice challenged with the rh strain of t. gondii by immunizing the mice with a eukaryotic vector expressing the protein rop18 of t. gondii. we are now looking for ways to improve this vaccination strategy and enhance protection. | 2015 | 25886737 |
| unique genetic features of canine adenovirus type 1 (cadv-1) infecting red foxes (vulpes vulpes) in northern norway and arctic foxes (vulpes lagopus) in svalbard. | canine adenovirus type 1 (cadv-1) is the aetiological agent of infectious canine hepatitis (ich) in domestic dogs (canis familiaris). in spite of the widespread use of vaccination, cadv-1 continues to circulate in the dog population. although a high number of serological screenings have indicated that cadv-1 is widespread in fox species, little is known about the potential role of foxes as reservoirs of cadv-1. furthermore, very little data exist on the molecular features of this virus in foxes. ... | 2019 | 30826932 |
| a serosurvey of multiple pathogens in american black bears (ursus americanus) in pennsylvania, usa indicates a lack of association with sarcoptic mange. | infectious diseases, particularly of wildlife, are intrinsically linked to human and domestic animal health. reports of sarcoptic mange in black bears (ursus americanus) are increasing in multiple states in the usa and while the reason is unknown, mange in other species has been associated with immunosuppression from multiple causes. serum from bears across pennsylvania were collected to determine the seroprevalence of five pathogens important for animal and/or human health: canine distemper vir ... | 2019 | 31547006 |
| 5-ht1b autoreceptors differentially modulate the expression of conditioned fear in a circuit-specific manner. | located in the nerve terminals of serotonergic neurons, 5-ht1b autoreceptors are poised to modulate synaptic 5-ht levels with precise temporal and spatial control, and play an important role in various emotional behaviors. this study characterized two novel, complementary viral vector strategies to investigate the contribution of 5-ht1b autoreceptors to fear expression, displayed as freezing, during contextual fear conditioning. increased expression of 5-ht1b autoreceptors throughout the brain s ... | 2015 | 25907441 |
| immunohistochemical evidence of canine morbillivirus (canine distemper) infection in coatis (nasua nasua) from southern brazil. | the pathological and immunohistochemical (ihc) findings associated with infection due to canine morbilivírus (canine distemper virus, cdv) are described in coatis (nasua nasua). tissue fragments of coatis (n = 13) that died at the bela vista sanctuary, paraná, southern brazil, were routinely processed for histopathology to identify the main histopathologic patterns as compared to that of the domestic dog. selected formalin-fixed paraffin-embedded (ffpe) tissue fragments of the lungs, liver, urin ... | 2020 | 32080984 |
| production of canine adenovirus type 2 in serum-free suspension cultures of mdck cells. | the potential of adherent madin darby canine kidney (mdck) cells for the production of influenza viruses and canine adenovirus type 2 (cav-2) for vaccines or gene therapy approaches has been shown. recently, a new mdck cell line (mdck.sus2) that was able to grow in suspension in a fully defined system was established. in this work, we investigated whether the new mdck.sus2 suspension cell line is suitable for the amplification of cav-2 under serum-free culture conditions. cell growth performance ... | 2015 | 25994255 |
| bioprocess development for canine adenovirus type 2 vectors. | canine adenovirus type 2 (cav-2) vectors overcome many of the clinical immunogenic concerns related to vectors derived from human adenoviruses (advs). in addition, cav-2 vectors preferentially transduce neurons with an efficient traffic via axons to afferent regions when injected into the brain. to meet the need for preclinical and possibly clinical uses, scalable and robust production processes are required. cav-2 vectors are currently produced in e1-transcomplementing dog kidney (dk) cells, wh ... | 2013 | 22763405 |
| sequence-independent vidisca-454 technique to discover new viruses in canine livers. | in many mammals, viruses cause hepatitis. despite many efforts a specific virus responsible for canine idiopathic hepatitis has not been identified. the discovery of a viral etiology in canine hepatitis will promote the development of specific drugs and vaccines for the treatment of idiopathic hepatitis in dogs. the objective of this study was the application of the sequence-independent virus discovery cdna-amplified fragment length polymorphism (vidisca) technique combined with high through-put ... | 2012 | 22664180 |
| molecular evidence for vaccine-induced canine distemper virus and canine adenovirus 2 coinfection in a fennec fox. | a 61-d-old fennec fox (vulpes zerda), 11 d after receiving a multivalent, modified-live virus vaccine containing canine distemper virus (cdv), canine adenovirus 2 (cadv-2), parainfluenza virus, parvovirus, and canine coronavirus, developed oculonasal discharge, and subsequently convulsions, and hemoptysis, and died. microscopic changes in the cerebrum were evident, including neuronal degeneration and necrosis; intracytoplasmic eosinophilic inclusion bodies were observed in astrocytes. cdv was de ... | 2020 | 32560597 |
| serum biochemistry values and select serologic screening of brown hyenas ( parahyaena brunnea) from the namib desert, namibia. | blood from 30 free-ranging brown hyenas ( parahyaena brunnea) was collected for biochemical analysis and select serologic screening in namibia from 1997 to 2010. age was found to have an influence on several biochemical parameters that may be related to growth, a developing immune system, and differences in diet. seasonal differences in diet of coastal brown hyenas also had an overall significant effect on lipemia values, and differences in stress due to varying capture methods could be associat ... | 2018 | 30592906 |
| a novel recombinant peste des petits ruminants-canine adenovirus vaccine elicits long-lasting neutralizing antibody response against ppr in goats. | peste des petits ruminants (ppr) is a highly contagious infectious disease of goats, sheep and small wild ruminant species with high morbidity and mortality rates. the peste des petits ruminants virus (pprv) expresses a hemagglutinin (h) glycoprotein on its outer envelope that is crucial for viral attachment to host cells and represents a key antigen for inducing the host immune response. | 2012 | 22623990 |
| adenoviruses of canine and human origins in stool samples from free-living pampas foxes (lycalopex gymnocercus) and crab-eating foxes (cerdocyon thous) in são francisco de paula, rio dos sinos basin. | the spread of enteric viruses of domestic animals and human beings to wild species can be facilitated by the resistance of these viruses on the environment and their ability to be transmitted by water and contaminated food. the health status of the populations of pampas foxes lycalopex gymnocercus) and crab-eating foxes (cerdocyon thous) is largely unknown and the landscapes occupied by these animals in southern brazil have been threatened by human occupation and expansion of agriculture. in thi ... | 2015 | 26270208 |
| novel adenoviruses and herpesviruses detected in bats. | samples from native hungarian or captive bats were tested by pcr for the presence of adenoviruses and herpesviruses. two novel adenoviruses from a common noctule (nyctalus noctula) and a greater horseshoe (rhinolophus ferrum-equinum) bat were detected. in captive egyptian fruit bats (rousettus aegyptiacus), dna from two novel herpesviruses was demonstrated. phylogenetic analysis facilitated provisional taxonomic placement of the newly detected viruses. such analysis and the existence of unique, ... | 2011 | 20813566 |
| novel adenoviruses detected in british mustelids, including a unique aviadenovirus in the tissues of pine martens (martes martes). | several adenoviruses are known to cause severe disease in veterinary species. recent evidence suggests that canine adenovirus type 1 (cav-1) persists in the tissues of healthy red foxes (vulpes vulpes), which may be a source of infection for susceptible species. it was hypothesized that mustelids native to the uk, including pine martens (martes martes) and eurasian otters (lutra lutra), may also be persistently infected with adenoviruses. based on high-throughput sequencing and additional sanger ... | 2017 | 28749327 |
| novel sequence variants of viral hexon and fibre genes in two dogs with canine adenovirus type 1-associated disease. | there is little information on sequence variation of canine adenovirus type 1 (cadv-1), the aetiological agent of infectious canine hepatitis (ich). this study reports hexon and fibre gene sequence variants of cadv-1 in a dog with systemic ich and a dog with the ocular form of the disease ('blue eye') in northern italy in 2013. one of the sequence variants matched a cadv-1 fox sequence previously detected in italy. | 2017 | 28671077 |
| detection of canine adenovirus 1 in red foxes ( vulpes vulpes) and raccoons ( procyon lotor) in germany with a taqman real-time pcr assay. | we developed a real-time (rt)pcr assay based on taqman probe technology for the specific detection of canine adenovirus 1 (cadv-1). the assay is able to detect three 50% tissue culture infectious dose/ml in cadv-1-containing cell culture supernatant. viral genomes were not amplified of canine adenovirus 2 or of several bovine, porcine, and avian adenoviruses. in silico analysis provided no indication of amplification of other heterologous genomes. the sensitivity of the real-time assay exceeded ... | 2017 | 28581363 |
| genome sequence of canine adenovirus type 1 isolated from a wolf (canis lupus) in southern italy. | canine adenovirus type 1 (cadv-1), a dna virus of the family adenoviridae, causes infectious canine hepatitis, a highly contagious disease primarily affecting canids. in this report, we describe the isolation and whole-genome sequence of a cadv-1 isolate from the liver of a free-ranging wolf (canis lupus). | 2017 | 28428308 |
| a novel recombinant canine adenovirus type 1 detected from acute lethal cases of infectious canine hepatitis. | in this study, canine adenoviruses (cadvs) from two acute fatal cases of infectious canine hepatitis (ich) were analyzed using molecular detection and sequencing of the pviii, e3, and fiber protein genes. pathological findings in affected dogs were typical for cadv-1 associated disease, characterized by severe centrilobular to panlobular necrohemorrhagic hepatitis and the development of disseminated intravascular coagulation in the terminal stages of disease. comparison of partial genome sequenc ... | 2017 | 28426340 |
| characterization of a novel adenovirus isolated from a skunk. | adenoviruses are a ubiquitous group of viruses that have been found in a wide range of hosts. a novel adenovirus from a skunk suffering from acute hepatitis was isolated and its dna genome sequenced. the analysis revealed this virus to be a new member of the genus mastadenovirus, with a genome of 31,848 bp in length containing 30 genes predicted to encode proteins, and with a g+c content of 49.0%. global genomic organization indicated skadv-1 was similar in organization to bat and canine adenovi ... | 2015 | 26189043 |
| comparison of two canine adenoviruses. | | 1969 | 4312040 |
| recombinant canine adenovirus type 2 expressing rabbit hemorrhagic disease virus vp60 protein provided protection against rhd in rabbits. | rabbit hemorrhagic disease virus (rhdv) is responsible for rabbit hemorrhagic disease (rhd), which is an acute, lethal and highly contagious disease in both wild and domestic rabbits. although current vaccines are highly effective for controlling rhd, they are derived from infected rabbit livers and their use is thus associated with safety and animal-welfare concerns. in this study, we generated a recombinant lentogenic canine adenovirus type 2 (cav2) vector expressing the rhdv vp60 gene, named ... | 2018 | 29291998 |
| a canine adenovirus type 2 vaccine vector confers protection against foot-and-mouth disease in guinea pigs. | vaccination is a key element in the control of foot-and-mouth disease (fmd). the majority of the antigenic sites that induce protective immune responses are localized on the fmd virus (fmdv) capsid that is formed by four virus-encoded structural proteins, vp1 to vp4. in the present study, recombinant canine adenovirus type 2 (cav2)-based fmd vaccines, cav-p1/3c r° and cav-vp1 r°, respectively expressing the structural p1 precursor protein along with the non-structural 3c protein or expressing th ... | 2018 | 29544690 |
| infectious canine hepatitis in a brown bear ( ursus arctos horribilis) from alaska. | we diagnosed infectious canine hepatitis in a free-ranging brown bear ( ursus arctos horribilis) cub from alaska, us, found dead in october 2015. intranuclear inclusion bodies were present in hepatocytes, and immunohistochemistry showed reactivity to adenoviral antigens. sequencing of the hexon protein of adenovirus showed 100% identity to canine adenovirus 1. | 2018 | 29498898 |
| transduction of craniofacial motoneurons following intramuscular injections of canine adenovirus type-2 (cav-2) in rhesus macaques. | reliable viral vector-mediated transgene expression in primate motoneurons would improve our ability to anatomically and physiologically interrogate motor systems. we therefore investigated the efficacy of replication defective, early region 1-deleted canine adenovirus type-2 (cav-2) vectors for mediating transgene expression of fluorescent proteins into brainstem motoneurons following craniofacial intramuscular injections in four rhesus monkeys (macaca mulatta). vector injections were placed in ... | 2019 | 31619971 |
| multicentric molecular and pathologic study on canine adenovirus type 1 in red foxes (vulpes vulpes) in three european countries. | canine adenovirus type 1 (cadv-1) is the agent of infectious canine hepatitis, a severe frequently fatal disease affecting primarily dogs (canis lupus familiaris). the virus has been detected in many wild carnivore species. our aim was to evaluate the prevalence and genetic and histopathologic features of cadv-1 in wild red foxes (vulpes vulpes). kidney and liver samples were obtained from 86 subjects, coming from the uk (n=21), italy (n=36), and germany (n=29). we used pcr, targeting the viral ... | 2019 | 31166851 |
| detection of selected pathogens in apennine wolf (canis lupus italicus) by a non-invasive gps-based telemetry sampling of two packs from majella national park, italy. | in this study, a multi-pathogens survey was conducted to verify the sanitary status of two italian wolf packs of majella national park. twenty fecal samples (10/pack) were collected using a sampling protocol, based on the combining data from radio-collared wolves with geographic information system (gis) analysis, allowing to mark off the home range of packs and to recover group-specific and high-quality specimens. virological screening against the most prevalent canine viruses (protoparvovirus, ... | 2019 | 32214950 |
| identification of pantropic canine coronavirus in a wolf ( canis lupus italicus) in italy. | we report a case in an italian wolf ( canis lupus italicus) of pantropic canine coronavirus infection, which has previously been detected only in dogs. the wolf was coinfected by canine parvovirus type 2b and canine adenovirus type 2, which highlighted the crucial role of epidemiologic surveys in european wild carnivores. | 2019 | 30376395 |
| sequential circulation of canine adenoviruses 1 and 2 in captive wild carnivores, france. | scarce data are currently available about the ecology of canine adenoviruses (cadvs) in wild carnivores. in this paper, the consecutive circulation of cadv-1 and cadv-2 in wild carnivores maintained in a french zoological park is reported. a fatal cadv-1 infection was observed in a eurasian wolf (canis lupus lupus), which displayed gross lesions, histopathological changes and immunohistochemical findings suggestive of cadv-1 infection. the virus was isolated on cell cultures and its genome was d ... | 2018 | 29981710 |
| first detection of canine parvovirus 2b dna in a crab-eating fox pup (cerdocyon thous, linnaeus, 1766). | the crab-eating fox (cerdocyon thous) is a small wild mammal present in all brazilian biomes and in some countries of south america. this study aimed to verify the involvement of viral infectious agents in the death of a wild crab-eating fox pup (cerdocyon thous) in brazil. the center for medicine and research of wild animals of the universidade estadual paulista received a free-living crab-eating fox aged approximately 21 days and apparently healthy. after 13 days, the animal presented anorexia ... | 2020 | 33095410 |
| wild canids, domestic dogs and their pathogens in southeast brazil: disease threats for canid conservation. | wild canids are under many pressures, including habitat loss, fragmentation and disease. the current lack of information on the status of wildlife health may hamper conservation efforts in brazil. in this paper, we examined the prevalence of canine pathogens in 21 free-ranging wild canids, comprising 12 cerdocyon thous (crab-eating fox), 7 chrysocyon brachyurus (maned wolf), 2 lycalopex vetulus (hoary fox), and 70 non-vaccinated domestic dogs from the serra do cipó national park area, southeast ... | 2010 | 32214695 |
| canine adenovirus type 1 (cadv-1) in free-ranging european brown bear (ursus arctos arctos): a threat for cantabrian population? | canine adenovirus type 1 (cadv-1) is responsible for infectious canine hepatitis. the disease has been described in captive american black bear (ursus americanus) and european brown bear (ursus arctos arctos), with just one recently reported case in a cub of a free-ranging brown bear (ursus arctos horribilis) from alaska. the aim of this work is to summarize findings related to presence and associated mortality of cadv-1 in 21 free-ranging cantabrian brown bears (ursus arctos arctos) submitted t ... | 2018 | 30179311 |