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purification and some properties of rat liver cysteine oxidase (cysteine dioxygenase).cysteine oxidase (cysteine dioxygenase, ec 1.13.11.20) was purified approximately 1000-fold from rat liver. the purified enzyme (protein-b) was obtained as an inactive form, which was activated by anaerobic preincubation with l-cysteine. the active form of protein-b was inactivated during aerobic incubation to produce cysteine sulfinate. this inactivation of protein-b was protected by a distinct protein in rat liver cytoplasm, namely stabilizing protein (protein-a). the ka and km values for l-cy ...19762307
mechanism of active shrinkage in mitochondria. ii. coupling between strong electrolyte fluxes.1. addition of succinate to valinomycin-treated mitochondria incubated in kcl causes a large electrolyte penetration. the process depends on a steady supply of energy and involves a continuous net extrusion of protons. rates of respiration and of electrolyte penetration proceed in a parallel manner. 2. a passive penetration of k+ salt of permeant anions occurs in respiratory-inhibited mitochondria after addition of valinomycin. addition of succinate at the end of the passive swelling starts an a ...19762314
a possible mechanism of the generation of singlet molecular oxygen in nadph-dependent microsomal lipid peroxidation.a simplified system, consisting of nadph, fe3+-adp, edta, liposomes, nadph-cytochrome c reductase and tris - hcl buffer (ph 6.8), has been employed in studies of the generation of singlet oxygen in nadph-dependent microsomal lipid peroxidation. the light emitted by the system involves 1deltag type molecular oxygen identifiable by its characteristic emission spectrum and its behavior with beta-carotene. the generation of another excited species (a compound in the triplet state) could be demonstra ...19762317
immunological similarity between nadh-cytochrome b5 reductase of erythrocytes and liver microsomes.in a number of animal species soluble nadh-cytochrome b5 reductase of erythrocytes was compared with membrane-bound nadh-cytochrome b5 reductase of liver microsomes by using an antibody to purified nadh-cytochrome b5 reductase from rat liver microsomes. the results obtained indicated clearly that they are immunologically very similar to each other. the data with erythrocyte ghosts suggested that cytochrome b5 and nadh-cytochrome b5 reductase are also present in the ghost.19762319
various properties of the creatine transport system and the location of creatine kinase in skeletal muscle mitochondria.water and creatine contents were studied in rat skeletal muscle mitochondria after their 5 min. incubation in creatine solutions, ph 7.2 or 8.4. the content of water and creatine in mitochondria was found to be higher at ph 8.4, than at ph 72, the creatine content correlated with the water content. structural creatine analogues, containing aminogroups with pka greater than or equal to 9.5 or carboxyl groups, inhibited the infusion of creatine into mitochondria more strongly than substances havin ...19752328
purification and some properties of electrophoretic variants of 6-phosphogluconate dehydrogenase from rat erythrocytes.purification procedure of electrophoretic variants ff and ss of 6-phosphogluconate dehydrogenase (6-pgd) is described. the method includes (nh4)2so4 fractionation and chromatography on deae- and cm-celluloses. isoenzymes were purified about 5000 fold, and were found to be homogenous by disc electrophoresis in 7% polyacrylamide gel. it was found by comparative studies of activities of variants ff and ss, that ph optimum was 8.2 for variant ff and 8.8 for variant ss. km for 6-phosphogluconate were ...19752334
on the proteolytic acitivity of ribosomes.the presence of a proteinase on polysomes, isolated from rat liver has been demonstrated. the proteinase was not removed from polysomes upon treatment of the latter with 0,5 m ammonium chloride. the ph optimum of the enzymes is at ph 7,0.19752337
assessment in the guinea-pig ileum and mouse vas deferens of benzomorphans which have strong antinociceptive activity but do not substitute for morphine in the dependent monkey.1 four benzomorphans which have potent antinociceptive activity in the hot-plate and writhing tests in the mouse but do not suppress or precipitate withdrawal symptoms in the morphine-dependent monkey, have been examined for their pharmacological actions in the guinea-pig ileum and mouse vas deferens. 2 in the guinea-pig ileum their agonist potencies are 1.5 to 400 times greater than that of normorphine of morphine whereas in the mouse vas deferens their potencies relative to morphine are 0.3 to ...19752359
letter: massive intoxication with metoprolol. 19762365
ph-dependent solubilization of brain mitochondrial hexokinase: developmental considerations. 19752368
value of the "gastric chamber", new technic performed ex vivo, for the study of the gastric mucosa of the rat.the "gastric chamber" technique, performed in the anaesthetised rat, enables the study of gastric mucosal fragility induced by doses of phenylbutazone, which do not themselves cause ulceration or exulceration. the perfusion of buffered solution at ph 2-8 into the gastric chamber shows that prior oral administration of phenylbutazone 50 mg/kg increases the fragility of the mucosa. the optimal delay separating this administration from the time of experimentation is 6 hours. the effects seen are es ...19752370
ciguatera: neurophysiologic and histoenzymologic studies of several fractions of ciguatoxic extracts.two fractions of ciguatoxic extracts were isolated by chromatography. the first showed anticholinesterasic properties, while the other acted directly on the muscular fibre. these findings were histoenzymologically confirmed. controls with non-toxic extracts of fishes from the coral benthos reinforce the theory of the ecological origins of ciguatoxins.19752376
monoamine storage sites in the rat superior cervical ganglion following synthesis inhibition.monoamine storage sites in paraganglionic (pg-) cells of the rat superior cervical ganglion were investigated by electron and fluorescence microscopy following treatment with p-chlorophenylalanine (pcpa), disulfiram or guanethidine respectively. dense core vesicles in pg-cells are significantly decreased (p less than 0.001) in number following pcpa, and in the majority of these cells following disulfiram and guanethidine. however in a minor portion of pg-cells the latter agents cause an increase ...19762382
ultracytochemistry of the synaptic ribbons in the rat pineal organ.the synaptic complexes of the rat pinealocytes are neither cholinergic nor adrenergic. in the synaptic vesicles, a neurotransmitter carrier substance of lipid nature reacting with oso4-zn i2 mixture (similar to that present in both cholinergic and adrenergic vesicles) was not found. in addition, there were no indications of glucose-6-phosphatase or thiamine-pyrophosphatase activity in the synaptic vesicles. thus, it appears that the synaptic vesicles do not originate from the rough or smooth end ...19762383
a phylogenetic study of serum nonsuppressible insulin-like activity (nsila). 19762424
drug-induced phospholipidoses. ii. tissue distribution of the amphiphilic drug chlorphentermine. 19752448
failure to monoaminergic and cholinergic receptor blockers to prevent prostaglandin e2-induced luteinizing hormone release.receptor blocking drugs were used to determine whether adrenergic, dopaminergic, serotoninergic, or cholinergic synapses are involved in mediating the lh release induced by intraventricularly injected pge2. prostaglandin e2 (5mug) was injected into the 3rd ventricle (3rd v) of ovariectomized rats, and plasma lh concentrations before and after treatment were determined by radioimmunoassay. phentolamine, 20 or 30 mug, or pronethalol, 20 mug (alpha and beta adrenergic receptor blockers, respectivel ...19762457
adrenergic binding sites and enzyme activities in the heart of hyperthyroid rats.in present study interactions of some adrenergic drugs with the binding of 3h-norepinephrine (ne) and response of some enzymatic systems in the heart of rats with pharmacological hyperthyroidism have been investigated. binding of ne to cardiac particles was inhibited by isoproterenol, propranolol and in lower concentrations by another beta-blocking drug trimepranol both in control and hyperthyroid hearts in the same degree. however, after addition of nonradioactive norepinephrine (10(-3) m) the ...19752459
fenitrooxon and s-methyl fenitrothion: acute toxicity and hydrolysis in mammals. 19752462
glutamine synthetase, glutaminase and phosphodiesterase activities in brain under hypoxia: in vitro effect of cortisol, gaba and serotonin on glutamine synthetase.the effect of hypobaric hypoxia on the activities of glutamine synthetase, glutaminase and cyclic 3'5' amp phosphodiesterase in rat brain was studied after exposure to 25,000' for 6 h. glutamine synthetase activity was increased in all the regions of brain studied, and addition of gamma amino butyric acid, serotonin and cortisol in vitro produced a differential response. glutaminase activity decreased in the whole brain. cyclic 3'5' amp phosphodiesterase activity decreased in cerebellum, medulla ...19752463
effect of triiodothyronine on rat liver chromatin protein kinase.1. injection of triiodothyronine to rats stimulates protein kinase activity in liver chromatin nonhistone proteins. a significant increase was found after two daily injections. a 4-fold increase was observed with the purified enzyme after eight daily injections of the hormone. no variations were observed in cytosol protein kinase activity. electrophoretic pattern, effect of heat denaturation, effect of p-hydroxymercuribenzoate seem to indicate that the enzyme present in treated rats is not ident ...19762475
subcellular distribution of histone-degrading enzyme activities from rat liver.chromatin prepared from liver tissue contains a histone-degrading enzyme activity with a ph optimum of 7.5-8.0, whereas chromatin isolated from purified nuclei is devoid of it. the histone-degrading enzyme activity was assayed with radioactively labelled total histones from ehrlich ascites tumor cells. among the different subcellular fractions assayed, only lysosomes and mitochondria exhibited histone-degrading enzymes. a ph optimum around 4.0-5.0 was found for the lysosomal fraction, whereas 7. ...19762476
effects on rectal temperature in rats of gamma-aminobutyric acid; possible mediation through putative transmitters.the rectal temperature of male rats was measured in a thermoneutral environment (25 degrees c) and at ambient temperatures of 15 and 35 degrees c. unless otherwise specified all drugs were administered intracerebroventricularly (i.c.v.) and all results are reported for the thermoneutral environment. exposure to 15 degrees c did not affect the rectal temperature but exposure to 35 degrees c produced hyperthermia. at 15 and 25 degrees c, 20 mug gaba produced hyperthermia which was longer lasting a ...19762482
the amphetamine-induced inhibition of dopamine biosynthesis in rat striatum.the dopamine biosynthetic machinery of intact synaptosomes of rat striatum showed a 5-fold increase in development from 3-day-old neonates to adults, and it was fully developed between 2-3 weeks after birth. concurring with this development was the appearance 2 weeks after birth of a regulatory mechanism(s) through which amphetamine in vivo induced an inhibition of dopamine biosynthesis. the inhibition was not appreciably reversed when haloperidol, in addition to amphetamine, was administered.19762484
distinctive histochemical characteristics of brain capillaries in organotypic culture. 19762486
regulation of increased acid proteinase in denervated skeletal muscle. 19762487
mechanics of vascular smooth muscle contraction. 19752488
gastric irrigation and secretion in the ligatured or fistuled pylorus rat upon influence of intra-duodenal olive oil (author's transl).pylorus ligature hides the inhibitory effects of endogenous cholecystokinine-pancreozyme (cck-pz) on gastric mucosal secretion and irrigation, whereas the juice collected through transduodenal pyloric fistula makes this phenomenon obvious. it appears that the pyloric fistula encourages inhibition of gastrinic secretion, so that the cck-pz can achieve its effects.19752489
ability of adrenergic nerves to accumulate exogenous noradrenaline under some pharmacological influences.by employing spectrofluorometry and quantitative fluorescent histochemistry the norepinephrine (ne) content in the tissue and in the adrenergic nerves of vas deferens of the rat after depletion of the transmitter's reserves with tyramine and their subsequent replenishment through incubation of the tissue with exogenous ne was measured. the object of investigations was also the influence of antidepressants (phthoracizine and imipramine) and of the cholinolytic spasmolytin (diphacyl) on processes ...19752494
regulation of citrate transport in rat liver mitochondria metabolizing pyruvate. 19762496
inhibition of dna synthesis in differentiating cardiac muscle by nad. 19762499
phosphorylation without protonmotive force. 19762503
recent studies on cytochrome p-450-linked functions in isolated rat liver cells.in rat liver cells isolated by perfusion in the perfusion in the presence of collagenase, the major portion of cytochrome p-450 is present in the oxidized, nonsubstrate-bound, low spin state. drug addition to a suspension of liver cells results in the rapid formation of the cytochrome p-450 (fe3+)-substrate complex which in turn is followed by the appearance of other species with different spectral characteristics before steady state drug monooxygenation is achieved. cytochrome p-450-linked meta ...19752526
solubilization and purification of a 3alpha-hydroxysteroid dehydrogenase in rat liver microsomes (author's transl).the microsomal 3-hydroxysteroid dehydrogenases were solubilized with lubrol, a non-ionic detergent. a 3alpha-hydroxysteroid dehydrogenase is purified about 100-fold by double affinity chromatography on 5alpha-dihydrotestosterone-sepharose. this enzyme can use both nadh and nadph as coenzymes.19762535
on the nature of the presumed receptor for ige on mast cells. iii. kinetics of the blocking of the pca reaction by cell-free particulate preparations from rat peritoneal mast cells and effect of ph and calcium concentration on the reaction.the 'binding' of ige to particulate preparations derived from sonicated purified rat mast cells was measured by the blocking of pca titrations of the supernatant solutions from incubations with such preparations. it was found that the pca blocking reaction was inhibited by the addition of calcium ion to the incubations. the blocking reaction was strongly dependent on the ph of the incubations, being maximal at ph values lower than 5-0. the blocking reaction proceeded in a linear manner for at le ...19762550
interaction of antihistaminic agents with adrenoceptors. 19752553
ganglion blocking activity of a few quaternised quinoline derivatives. 19752555
central regulation of blood eosinophilia by the beta-adrenergic system in rats.the effect of isoprenaline and three different beta-adrenergic blocking agents on blood eosinophilia was evaluated in the rat. eosinopenic effect of isoprenaline was antagonized by intraperitoneal pretreatment with propranolol and by high but not low doses of practolol. sotalol, a beta-blocker which in contrast to propranolol does not penetrate the blood-brain barrier, did not affect the eosinophil count when injected intraperitoneally, but produced a marked increase in number of circulating eos ...19762558
the effect of aluminum and ph on altered body distribution of 99mtc-ehdp. 19762562
a histochemical study of the apparent deamination of proteins by sodium hypochlorite.the possible chemical mechanisms by which neutral solutions of sodium hypochlorite containing a high concentration of sodium chloride abolish the acidophilia of proteins in sections of fixed tissue are reviewed. the most probable one is the chlorination of the protein terminal amino groups, followed by the breakdown of the n-chloramine so formed into alpha-ketocarboxylic acid, nitrile or aldehyde groups. hypochlorite solutions certainly do not deaminate tissue sections as was previously thought. ...19752567
effects of dbcamp and theophylline on rat adrenal medulla grown in tissue culture.explants of rat adrenal medulla were grown in tissue culture. the effects of various doses of dbcamp ranging from 0.001 mm up to 1 mm and equimolar amounts of theophylline were recorded by phase contrast optics and catecholamine histochemistry (glyoxylic acid method) over six days. there was a dose-dependent inhibition of the normally occurring outgrowth of schwann cells, "chromaffin" cells and axons from the explants. maintenance of glyoxylic acid-induced fluorescence in "chromaffin" cells was ...19762568
improved histological localization of gaba-transaminase activity in rat cerebellar cortex after aldehyde fixation.a method for the chemical fixation of the enzyme gaba-transaminase in nervous tissue is described. it is shown that after perfusion with a formaldehyde/glutaraldehyde fixative, activity of the enzyme in cerebellar cortex is demonstrable whilst cellular morphology is preserved. results from the improved technique have shown new sites of gaba-transaminase activity in cerebellar cortex. in view of these results a special function for glial cells in this area of brain has been suggested.19762570
autooxidation and hydroxylation reactions of oxygenated cytochrome p-450cam.oxy-ferrous substrate-bound cytochrome p-450cam (mrso2) autooxidizes in the absence of its specific effector protein, putidaredoxin, without hydroxylating the substrate, camphor. the autooxidation is first order with an activation energy of 17 kcal mol-1 at 25 degrees, ph 7.0. substrate removal and low ph accelerate the reaction. the product, 5-exo-oh camphor, and a nonhydroxylated pseudosubstrate, norcamphor, stabilize the complex in a manner similar to camphor. increased oxidation rate of mrso ...19762601
stoichiometry of h+ ejection during respiration-dependent accumulation of ca2+ by rat liver mitochondria.we have investigated the energy-dependent uptake of ca2+ by rat liver mitochondria with succinate as respiratory substrate with rotenone added to block nad-linked electron transport. in the presence of 3-hydroxybutyric or other permeant monocarboxylic acids ca2+ was taken up to extents approaching those seen in the presence of phosphate. the quantitative relationship between cation and anion uptake was determined from the slope of a plot of 3-hydroxybutyrate uptake against ca2+ uptake, a method ...19762609
effects of adrenalectomy and glucocorticoid therapy on enzyme activities in mammary and adipose tissues from lactating rats.several mammary and adipose enzymes were measured in normal, adrenal-ectomized, adrenalectomized cortisol-treated, and intake-restricted lactating rats. acetyl-coa carboxylase, lipoprotein lipase, and triglyceride synthetase complex activities in mammary tissue were unchanged by intake restriction, decreased by adrenalectomy, and increased by glucocorticoid-replacement therapy. malic dehydrogenase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and lipoprotein lipase activi ...19762627
lymphoid cell fractionation by aggregated immunoglobulin-agarose columns.fractionation by columns of aggregated rat immunoglobulin (agg ig)-agarose was investigated as a method of separating different populations of lymphoid cells. with rat spleen cells, agg ig columns retained phagocytes, igm- and igg-antibody-forming-cells, cells mediating antibody- or pha-induced lysis of chicken erythrocytes, and specifically immune splenocytes lytic to chicken erythrocytes without exogenous antibody. agg ig columns did not selectively remove 'b lymphocytes' (surface-ig-bearing l ...19762637
inhibition of drug-induced anorexia in rats by methysergide.iproniazid was found to reduce food consumption in fasting rats. combined treatment of iproniazid with tryptophan resulted in a significantly greater anorexic action whilst tryptophan alone had no effect on food consumption. iproniazid treatment was associated with a significant increase in brain 5-hydroxytryptamine (5-ht) concentration but in association with tryptophan higher brain 5-ht concentrations were recorded. the anorexic action of the iproniazid-tryptophan combination was antagonized i ...19752658
the quantification of kanamycin ototoxicity in the rat using conditioned tone discrimination.twelve male lister hooded rats were conditioned to discriminate an 8 khz tone (56.5 +/- 0.5 db re 0.0002 dyne cm-2) and were subsequently injected subcutaneously with kanamycin (400 mg kg-1 day-1) for 28 days; during this time and for five weeks after dosage was stopped, the nature and extent of the resultant hearing deficits were studied. the animals' preyer reflex thresholds were determined at intervals during the study. only one rat was unaffected by the kanamycin dosage. the onset of hearing ...19752661
the 6-hydroxydopamine rotational model for the detection of dopamine agonist activity: reliability of effect from different locations of 6-hydroxydopamine. 19752668
effect of catechol-o-methyltransferase inhibitors on brain apomorphine concentrations and stereotyped behaviour in the rat. 19752669
failure of depletion of rat brain 5-hydroxytryptamine to alter fenfluramine-induced anorexia. 19752670
influence of the adrenals and gonads on the plasma kininogen concentrations in male and female rats. 19752671
placental transfer of carbamazepine in the rat. 19752672
effects of several catecholamine-derived tetrahydroisoquinolines on the hypogastric nerve-vas deferens preparation of the rat. 19752673
the absorption of benorylate from everted sacs of rat intestinal. 19752674
mass spectrometric identification of a new metabolite of pethidine. 19752680
proceedings: the effects of amantadine, tranylcypromine, amphetamine and tyramine on the response of the rat anococcygeus muscle after bretylium. 19752686
proceedings: the effects of buprenorphine, morphine and pentazocine on turning behaviour and stereotypy induced by apomorphine in the rat. 19752687
proceedings: methylamphetamine withdrawal as a model for the depressive state: antagonism of post-amphetamine depression by imipramine. 19752688
proceedings: the use of conditioned tone discrimination to study kanamycin ototoxicity in the rat. 19752689
proceedings: potential tolerance to beclamide and related drug interactions. 19752690
proceedings: anti-inflammatory properties of dapsone. 19752693
proceedings: studies of experimental chronic renal failure i, increased permeability of the rat's blood-brain barrier to phenglutarimide hydrochloride. 19752710
proceedings: variations in the concentrations of monamines and their metabolites in eight regions of rat brain during the oestrous cycle: a basis for interactions between hormones and psychotropic drugs. 19752713
proceedings: a possible interaction between the 24h rhythms in catecholamine and 5-hydroxytryptamine concentration in the rat brain. 19752715
proceedings: the effects of some bis-quaternary ammonium compounds on choline acetyltransferase, cholinesterase and the cholinergic receptor. 19752737
proceedings: the effects of some analogues of hemicholinium at the neuromuscular junction. 19752739
proceedings: the synthesis of some piperidinol compounds as potential inhibitors of cholinergic transmission. 19752740
proceedings: an investigation of the rat in situ absorption model using practolol and propranolol. 19752741
proceedings: influence of a mucin model system upon the bioavailability of phenylbutazone and warfarin sodium from the small intestine. 19752751
synthesis of angiotensin ii antagonists containing n- and o-methylated and other amino acid residues.1-n-methylisoasparagine,8-isoleucine- (i), 1-sarcosine,4-n-methyltyrosine,8-isoleucine- (ii), 1-sarcosine,5-n-methylisoleucine,8-isoleucine- (iii), 1-sarcosine,8-n-methylisoleucine- (iv), 1-sarcosine8k-n-methylisoleucine,8-n-methylisoleucine- (v), 1-sarcosine,8-o-methylthreonine- (vi), 1-sarcosine,8-methionine- (vii), and 1-sarcosine,8-serineangiotensin ii (viii), synthesized by merrifield's solid-phase procedure, possess respectively 0.8, 0.3, 0.5, 1.0, 0.0, 0.5, 3.7, and 0.7% pressor activity ...19762777
proceedings: glutamine synthetase and glutamine gamma-glutamyl transferase in human, canine, and rat kidneys. 19752787
effect of the chronic action of small doses of ionizing radiation of the excretion of gaseous metabolic products in albino rats.white rats were kept in sealed chambers for 3 months and were exposed to chronic gamma-irradiation (100 mur/day). the effect of the exposure on the excretion of some metabolites--ammonia, acetone, carbon monoxide, phenol and organic substances measured by their oxidability--was studied. under the influence of low doses of gamma-irradiation the excretion of acetone and organic substances decreased, whereas that of carbon monoxide, phenol and ammonia increased. possible mechanisms responsible for ...19762802
effect of hyperoxia and hypokinesia on the formation and excretion of gaseous metabolic products in rats.it has been shown experimentally that a 30-day exposure of white rats to hypokinesia and moderate hyperoxia decreases elimination of ammonia and increases the formation and release into an enclosed atmosphere of carbon monoxide, aldehydes and ketones. the level of metabolism of prophyrin and nitrogen containing compounds as well as of fats and carbohydrates is higher during a combined effect of hypokinesia and moderate hyperoxia than during their separate influences.19762803
dietary caffeine and alcohol consumption by rats.the alcohol consumption of malnourished rats increased slowly but substantially during a 4-week period in which caffeine was added to their marginally adequate diet. consumption fell to precaffeine levels as soon as caffeine was withdrawn.19762809
ornithine decarboxylase activity and dna synthesis in morris hepatomas 5123-c and 7800. 19762827
purification of tyrosine hydroxylase from pheochromocytoma tumors. 19762828
5-hydroxytryptamine binding to synaptic membranes from rat brain. 19762829
insulin receptors of skeletal muscle: specific insulin binding sites and demonstration of decreased numbers of sites in obese rats.a membrane preparation was obtained from rat striated muscle. the preparation used has been shown to contain plasma membranes by electron microscopy as well as by enrichment in specific activity of both a plasma membrane enzyme "marker" (5'-nucleotidase) and cell surface 125i-incorporated radioactivity. the characteristics of 125i-insulin binding to this striated muscle preparation were studied, and it was found that 125i-insulin readily and specifically binds to this membrane preparation. the b ...19762834
myo-inositol oxygenase from oat seedlings.enzyme preparations from oat seedlings showing the activity of myo-inositol oxygenase (e.c.1.13.99.1) have been described previously. in contrast to myo-inositol oxygenase preparations from other sources, e.g. rat kidney or yeast, the oat enzyme seemed to exhibit a somewhat less stringent activity, acting on other inositols and inositol methyl ethers as well as on myo-inositol. by purification of the enzyme present in the extract from oat seedlings with the help of an affinity gel specific for e ...19762857
isolation of rat liver chromatin non-histone protein.a chromatin with the protein/dna ratio of 3.0 was obtained from rat liver cells nuclei. the chromatin was dissolved in 2 m nacl, ph 7, and reprecipitated by decreasing the ionic strength to 0.4 and increasing ph to 9.0. a fraction of non-histone proteins (nh-1) remained in a supernatant solution, the nh-1/dna ratio being equal to 1.3 and the ratio of acidic to basic aminoacid residues equal to 1.31. after chromatography on bio-rex 70 cation-exchang resin, a fraction (nh-2) with the nh-2/dna rati ...19752862
possibilities and limitations of uterine contraction inhibiting agents. 19762884
the anxiolytic effect of small cumulative doses of oxypertin in rats during the anxiety test and their possible neurochemical correlates in the hypothalamus and striatum. 19762888
mechanism of action of anticonvulsants. role of the differential effects on the active uptake of putative neurotransmitters.the effect of pentobarbital and phenytoin on the high-affinity uptake of the putative neurotransmitters gamma-aminobutyric acid (gaba), glutamate, and norepinephrine was examined in synaptosomes prepared from rat brain. both pentobarbital and phenytoin inhibited the uptake of norepinephrine. pentobarbital increased the uptake of gaba twofold and only slightly increased the uptake of glutamate. phenytoin facilitated gaba uptake to a lesser extent than did pentobarbital, but also increased the upt ...19762891
anti-hypertensive action of tenormine in the genetically hypertensive rat. 19752904
formation and in-vivo-distribution of different 99mtc-sn-pyrophosphate complexes.the formation of two different 99mtc-sn-pyrophosphate complexes was determined by evaluation of their in vivo distribution in rats. it was found that formation of a 2 : 2 sn : pyp complex, which is bone seeking, is depending as well on the pyrophosphate as on the hydrogen ion concentration in the equilibrium. on the contrary, formation of a 2 : 1 sn : pyp complex, which shows no bone affinity but concentrate in the kidneys, is hydrogen ion independent and occurs even at very low pyrophosphate co ...19752905
animal phospholipases (author's transl). 19762906
renin and hemodynamic changes via central adrenergic, cholinergic, and sodium receptor mechanisms in conscious rats.the effects of centrally administered autonomic drugs and hypertonic saline on renin release were studied in the conscious rat. a 0.3 mug intraventricular dose of isoproterenol, which is one-thirtieth of the intraperitoneal dose required to stimulate renin release, induced the release of renin into the systemic circulation. norepinephrine had no effect on renin release in the same dose range. hypertonic saline and carbachol suppressed renin release. alterations in renin release were preceded by ...19762926
regulation of proinsulin synthesis in isolated rat islets.(1) a system is described for studying the short-term effects of agents on proinsulin synthesis in vitro, as measured by the incorporation of 3hleucine into isolated proinsulin. (2) of the agents tested, glucose has the most marked, and apparently earliest, effect on proinsulin synthesis. (3) the adenyl cyclase system participates in the regulation of proinsulin synthesis since exogenous cyclic amp, glucagon, and caffeine are stimulatory. when cyclic amp is added to the medium in the presence of ...19762929
plasma prolactin levels in maturing intact and cryptorchid male rats: development of stress response.in order to determine the possible role of the seminiferous tubules in the regulation of prolactin secretion during sexual development, male rats were rendered cryptorchid at 22 days of age, and thereafter different groups of animals were decapitated at 8-10 day intervals between day 32 and day 70. cryptorchid rats showed destruction of the germinal epithelium accompanied by increased plasma fsh and, to a much lesser extent, increased plasma lh titers. nevertheless, plasma prolactin levels were ...19762934
dehydrogenation of reduced pyridine nucleotides by leydig cell tumors of the rat testis.the activities of the cytochrome c reductases and of the d-t diaphorase in rat leydig cell tumors have been described. the increase in enzymatic activity of the nadh cytochrome c reductase activity in functional tumors derived from interstitial cells of the rat testis is interpreted as being possibly related to hydroxylation of steroids by the neoplastic cells. meanwhile, the increase in the activity of the d-t diaphorase in the other tumor is interpreted as being an anaplerotic reaction to subs ...19762936
comparative study of the nonspecific pharmacodynamics of some neuroleptics and minor tranquilizers during long-term treatment on the reactivity of the genital tract. 19752938
effects of antianxiety drugs on the food intake in trained and untrained rats and mice (author's transl).various minor tranquilizers (benzodiazepines, barbiturates and meprobamate) induced an increase in the food intake of rats or mice. drugs were injected i.p. 30 min before testing and the amount of food consumed during 30 min was recorded. the enhanced food consumption occurred when the animals were in a novel situation, in a situation which they had previously experienced, or in their home cage, in which they were used to eating in the daytime within 30 min. studies with two benzodiazepines show ...19752942
lsd as an agonist at mesolimbic dopamine receptors.the dopamine agonist apomorphine (1.0 mg/kg i.p.) produced an enhanced stimulation of locomotor activity compared to control animals in rats injected bilaterally 14 days previously with 6-hydroxydopamine (6ohda) into the nucleus accumbens. (+)-lysergic acid diethylamide (lsd) also produced a marked stimulation of locomotor activity in the 6ohda treated animals at a dose (1.0 mg/kg i.p.) which was ineffective in control rats. (+)-bromo-lysergic acid diethylamide (2.0 mg/kg i.p.) did not stimulate ...19752943
effects of various inhibitors of tyrosine hydroxylase and dopamine beta-hydroxylase on rat self-stimulation after reserpine treatment.the behavioral effects of low doses of the catecholamine (ca) synthesis inhibitor, alpha-methyl-p-tyrosine (alpha-mpt, 50 mg/kg i.p.), or the norepinephrine (ne) synthesis inhibitors (fla-63, 15 mg/kg i.p., u-14624, 50 mg/kg i.p., or disulfiram 150 mg/kg i.p.) were studied in rats pretreated with reserpine (1 mg/kg i.p.) 24 h before. rats were implanted either in the area ventralis tegmenti (avt) or in the lateral hypothalamus (lh). the modifications of ca synthesis and endogenous ca levels were ...19762944
distribution of prostaglandin e 9-ketoreductase and nad+-dependent and nadp+-dependent 15-hydroxyprostaglandin dehydrogenase in the renal cortex and medulla of various species.regional distributions of pge 9-ketoreductase and 15-hydroxy-prostaglandin dehydrogenase were examined in the cytoplasmic fractions from the kidneys of seven species. all species contained an nadph-dependent reductase, as well as nad+- and nadp+-dependent dehydrogenases in both cortex and medulla. a previously unrecognized cytoplasmic nadh-dependent pge 9-ketoreductase was also detected in the cortex and medulla of rat and bovine kidney. total nad+- and nadp+-dependent dehydrogenase activity was ...19752961
time-dependent alterations in testicular function after experimental cryptorchism. 19752991
effect of lysyl oxidase inhibition of healing wounds. 19752992
the host component of the graft-versus-host reaction. a study on the popliteal lymph node reaction in the rat.i have measured the proportion of host cells in a popliteal lymph node undergoing a graft-versus-host (gvh) reaction. by using cytotoxic alloantisera with specificity for donor and host ag-b determined antigens, it was found that about 90% of live cells suspended from the node were of host origin. the role of host t lymphocytes in this reaction was evaluated by investigating gvh-reactivity in hosts depleted of t cells (b rats). these rats differed very little from normal hosts with regard to lym ...19763001
arboviruses isolated from mosquitos and man in surinam.in the capital of surinam, and in parts of the coastal plain and the savannah belt 47 strains of arbovirus were isolated from mosquito pools and from sentinel mice. most of the strains (51%) were of the mucambo type. the other types were guama, bimiti, una, catu, restan, maru, and kwatta. culex portesi was found to be the principal vector of all but one (maru) types. other vectors found to be carriers of arboviruses were psorophora ferox, mansonia venezuelensis, and aëdes serratus. the highest p ...19753002
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