| the thin-layer isoelectric focusing of lactate dehydrogenase isoenzymes in rabbit lens parts and in intraocular tissues. | lactate dehydrogenase (ldh) isoenzymes of rabbit lens and other intraocular tissues are separated by thin-layer isoelectric focusing and localized as discrete groups of multiple bands with defined isoelectric points after staining by the tetrazolium method. in the rabbit lens parts, the predominant isoenzymes are ldh-4 and ldh-5. the bands show microheterogeneity, are composed of 2-4 subcomponents, and the pattern shows a distribution of the liver type. the activity of the ldh-4 decreases and th ... | 1977 | 19982 |
| the isolation and characterization of a colony stimulating factor from human lung. | serum-free conditioned medium from human lung obtained at autopsy provides a rich source of colony stimulating factor which stimulates granulocytic and macrophagic colony growth in both mouse and human bone marrow. the appearance of the factor is enhanced by endotoxin and inhibited by either puromycin or actinomycin d. human lung colony stimulating factor is stable at the ph range of 6.5-10 and temperature of 56 degrees c for 30 min. it is resistant to trypsin and neuraminidase but is sensitive ... | 1977 | 20157 |
| some features of cyclic adenosine monophosphate metabolism in mouse liver and hepatoma 22. | the levels of cyclic adenosine monophosphate (camp) and two forms of camp phosphodiesterase with low (pde1) and high (pde2) affinity for the substrate were determined in homogenates from mouse liver and transplanted hepatoma 22. the level of camp in the tumour is 3 times lower than that in liver. by te kinetic parameters (vmax, km, ph optimum) adenylate cyclase from tumour does not show any significant differences as compared to the liver enzyme; the enzyme from hepatoma is, however, more sensit ... | 1977 | 20168 |
| xenogeneic neoplastic transplants in adult rats pretreated with thymic foetal extracts. | the paper deals with the effect of thymic extracts from young foetal pigs and calves on the development of tumour xenografts, i.e. mouse ascites ehrlich carcinoma and hamster malignant melanoma, in rats. the results of experiments showed that ehrlich carcinoma developed in 4 out of 72 animals, i.e., in those animals which had received thymic extracts from young foetal pigs, ehrlich carcinoma led to their death. ehrlich carcinoma assumed a solid form and the cells changed in their shape. it devel ... | 1977 | 20339 |
| pathogenesis of cryptorchidism. | cryptorchidism was induced experimentally by treating pregnant mice on the 14th day of pregnancy with 5 mg estrogen. testes from cryptorchid and control newborn and adult mice were investigated with radioimmunoassay and electron microscopy. it was concluded that a normal leydig cell function plays a decisive role in testicular descent. in cryptorchidism, leydig cells at birth are atrophic. testicular testosterone content is diminished as compared to controls. ultrastructural alterations of leydi ... | 1977 | 20399 |
| differences in mouse interferons according to cell source and mode of induction. | mouse interferon induced by ultraviolet-irradiated newcastle disease virus or polyriboinosinic-polyribocytidylic acid in t lymphocytes, b lymphocytes, macrophages, and primary mouse embryonic cell culture was studied. irrespective of the inducer, interferons produced by t or b lymphocytes were relatively heat stable and of low antigenicity when reacted with antiserum against l-cell interferon (ali), whereas interferons produced by macrophages and mouse embryo cells were heat labile and of high a ... | 1977 | 20403 |
| the stability of tyrosine aminotransferase and other proteins in enucleated rat hepatoma tissue culture cells. | the role of the nucleus in bringing about the induction of tyrosine aminotransferase (tat) by glucocorticosteroid hormone and its deinduction upon steroid removal has been studied in enucleated rat hepatoma tissue culture cells (fu5-5). both processes require the presence of the nucleus. however, cytoplasts from preinduced cells show an initial rapid decline in enzyme activity immediately after enucleation followed by maintenance of a constant level of activity. this initial decline in enzyme ac ... | 1977 | 20448 |
| chromatographic separations of alphavirus strains by hydroxylapatite. | hydroxylapatite column chromatography methods were developed to characterize selected alphavirus populations. different conditions of ph and phosphate molarity were required to obtain satisfactory elution profiles and separations for western equine encephalomyelitis virus strains, compared with eastern equine encephalomyelitis virus and semliki forest virus strains. raising the ph of the buffers effected earlier elutions of all viruses. selection of phosphate gradients with more gentle slopes an ... | 1977 | 20452 |
| mouse spinal cord in cell culture. iii. neuronal chemosensitivity and its relationship to synaptic activity. | 1. mouse spinal cord (sc) cells in dissociated cell cultures showed strong electrophysiologic responses to glutamate, gamma-aminobutyric acid (gaba), and glycine when these were iontophoretically applied to the neurons. 2. the extrapolated reversal potential for the glutamate response was 20-30 mv negative in contrast to the positive extrapolated reversal potential for the sc-sc excitatory postsynaptic potential. the data are interpreted as indicating different ionic mechanisms for the glutamate ... | 1977 | 20485 |
| specific binding sites for a parvovirus, minute virus of mice, on cultured mouse cells. | the early interactions between parvoviruses and host cells have not been extensively described previously. in this study we have characterized some aspects of viral binding to the cell surface and demonstrated the existence of specific cellular receptor sites for minute virus of mice (mvm) on two murine cell lines that are permissive for viral growth. the interaction had a ph optimum of 7.0 to 7.2, and both the rate and extent of the reactions were slightly affected by temperature. mouse a-9 cel ... | 1977 | 20517 |
| effect of age of mouse recipients on the functional activity of transplanted hematopoietic and lymphoid cells. | when transplanting the bone marrow cells from adult c57bl mice to the lethally irradiated (cba x c57bl) f1 hybrids of different age, the decrease of the colony forming activity of the stem haemopoietic cells was observed in the spleen of the older recipients, as compared with the 3 months old ones. the joint transplantation of the bone marrow and thymus cells resulted in both the cases in the stimulation of the growth of colonies. the number of endogenous colonies of haemopoietic cells arising i ... | 1977 | 20594 |
| embryologic development of a mouse sympathetic ganglion in vivo and in vitro. | the morphologic and biochemical development of the embryonic mouse superior cervical ganglion was characterized in vivo and in tissue culture. from 13 days of gestation, when the superior cervical ganglion was first visible, to birth at 19 days, tyrosine hydroxylase tyrosine 3-monooxygenase; l-tyrosine, tetrahydropteridine:oxygen oxidoreductase (3-hydroxylating); ec 1.14.16.2 activity increased 100-fold in vivo. explants of ganglia from 14-day embryos exhibited abundant neurite outgrowth in basa ... | 1977 | 20628 |
| antimuscarinic properties of antidepressants: dibenzepin (noveril). | the antimuscarinic potency of dibenzepin (noveril) was estimated by measuring (a) central in vivo effects in mice (antihypothermia and antitremor, both induced by oxotremorine), (b) peripheral in vivo activity (mydriasis caused by systemic administration of the drug), (c) the effects of dibenzepin on isolated smooth muscle from guinea pig ileum, and (d) in vitro determination of the affinity constant of dibezepine toward the muscarinic binding sites in whole mouse-brain homogenate. the data allo ... | 1977 | 20647 |
| evidence of decarboxylation of lysine by mammalian ornithine decarboxylase. | in enzymic preparations from mouse kidney stimulated with the anabolic steroid durabolin (nandrolone phenpropionate) lysine and ornithine were shown to inhibit the decarboxylation of each other competitively. the michaelis constants for the decarboxylations were approximately equal to the inhibition constants of the two amino acids. the ph optima of the decarboxylation of lysine and ornithine were found to be identical. chromatographic studies of the enzyme preparation on a sephadex g-150 superf ... | 1977 | 20736 |
| non-infectious antigen for laboratory diagnosis of japanese encephalitis. | sucrose-acetone suckling mouse brain antigens of na-kayama nih, jagar-01 and peking-1 strains of japanese encephalitis (je) virus treated with beta-propiolactone (bpl) retained their ability to specifically react in haemagglutination inhibition (hi) tests with antibody to je virus, as demonstrated with convalescent sera. bpl treatment abolished the infectivity but did not affect the haemagglutinating activity of the virus of the ph optimum of the haemagglutination (ha) test. | 1977 | 20767 |
| halothane-induced alterations of cyclic nucleotide concentrations in three regions of the mouse nervous system. | | 1977 | 20825 |
| changes in the electrical activity of myocardial fibers following heart transplantation to under the skin of the ear in mice. | intracellular rest and action potentials (rp and ap, respectively) of mouse heart graft were reduced on the 3rd--4th days after the transplantation as compared with the intracellular potentials of newborn mice. beginning from the 5th--6th days there occurred a gradual increase of the intracellular potentials of amplitude. the firt 7--8 days, both in the case of allograft and heterograft, the changes in the intracellular activity were the same. then in heterograft there occurred a repeated reduct ... | 1977 | 21006 |
| changes in the h-alloantigen-recognizing function of mouse lymphoid cells following hydrocortisone administration. | the capacity of spleen, thymus, and bone marrow cells of intact (control) and of hydrocortisone-treated mice cba to induce the lymph node type of graft-v-host reaction (gvhr) in hybrids f1 (cba x c57bl) was studied. after hydrocortisone injection (2.5 mg per mouse) the donor spleen cells became more active in gvhr, considering the value of lymph node indices and immunoblast content in the regional lymph node as compared with a control group. following transplantation of thymus cells taken from t ... | 1977 | 21008 |
| the permissive role of glucocorticoids in the development of ethanol dependence and tolerance. | in mice chronically treated with ethanol (in a liquid diet containing 6% ethanol ad libitum for 2 weeks), brain tryptophan hydroxylase (tph) activity was increased (by 30-45% in whole brain), while brain tyrosine hydroxylase activity remained unchanged. such chronic ethanol treatment also induced susceptibility to audiogenic seizures during withdrawal (60% incidence). when ethanol treatment was given to adrenalectomized (adx) mice, the increase of brain tph activity and the development of withdr ... | 1977 | 21065 |
| inhibition of fatty acid synthesis and stimulation of glucose release by angiotensin ii and adrenaline in the perfused mouse liver proceedings. | | 1977 | 21114 |
| enumeration of t rosette-forming cell population in rabbit peripheral blood using human, sheep, guinea-pig, rat, mouse and chicken red blood cells under different experimental conditions. | attempts were made to estimate peripheral t-lymphocytes in rabbits by their ability to form rosettes with sheep, human, guinea-pig, rat, mouse and chicken rbc. the reaction was studied under different conditions of ph and temperature. the test was also carried out in the presence or absence of glutaraldehyde. with sheep rbc, lymphocytes from 2 of the 5 rabbits studied formed rosettes to the extent of 1.5% in the system containing glutaraldehyde. these results however were not reproducible when s ... | 1977 | 21220 |
| a sex difference in the interaction between promethazine and morphine in the mouse. | the effects of promethazine on the antinociceptive and respiratory actions of morphine have been examined in the mouse. moderate doses of promethazine (5 and 10 mg kg-1) potentiated morphine's action in male mice but inhibited it in female mice. gonadectomy abolished the interaction between promethazine and morphine in both sexes, although the intensity and duration of morphine's activity was greatly enhanced in these mice. replacement of oestradiol in ovariectomized mice restored morphine's act ... | 1977 | 21241 |
| monoamine oxidase and catechol-o-methyl transferase activity in tetrahymena. | tetrahymena pyriformis strain hsm was found to have monomine oxidase (mao) and a catechol-3-methyl transferase-like (comt) activity. as in mammalian tissues, the mao activity is predominantly localized in the mitochondrial pellet and comt in the cytosol. the comt-like activity was present in amounts comparable to several mouse tissues and was inhibited by tropolone. mao activity was much lower than in any of the mouse tissues tested, and its activity varied greatly from preparation to preparatio ... | 1977 | 21289 |
| bacterial stimulation and granulocyte inhibition of granulopoietic factor production. | we attempted to determine the effect of live bacteria (staphylococcus epidermidis) on granulocyte colony-stimulating-factor production by human peripheral blood mononuclear cells (monocytes and lymphocytes) in vitro. addition of bacteria to mononuclear-cell cultures enhanced colony-stimulating-factor production by these cells, as assayed on both human and mouse bone marrow. addition of peripheral blood granulocytes to parallel cultures eliminated this enhancement effect, presumably by bacterial ... | 1977 | 21349 |
| oxidative metabolism and de-ethynylation of 17alpha-ethynylestradiol by baboon liver microsomes. | incubations of tritiated 17alpha-ethynylestradiol (ee2) with liver explants of baboon and mouse showed the primate species to be more efficient in the removal of the ethynyl group. liver microsomes from sexually immature male and female baboons were then incubated with tritiated ee2 and estradiol (e2). each hormone bound irreversibly to the microsomal pellet. addition of glutathione reduced the irreversible or covalent association. incubations with e2 demonstrated significant conversion to estro ... | 1977 | 21474 |
| acid elution of blood group antibodies from intact erythrocytes. | human igm and igg antibodies against blood group antigens (a, b, d, c, c, e, e, fya, k), autoantibodies and mouse igm and igg antibodies against sheep erythrocytes have been eluted from intact human and sheep red cells by glycine-hcl buffer, ph 3.0. the yield of human antibodies was higher with acid than with heat and ether elution, and the contamination of hemoglobin in the eluate was negligible. the acid elution method is very simple and rapid and, therefore, highly suitable for experimental a ... | 1977 | 21486 |
| kinetics of catecholamine sensitive na+-k+ atpase activity in mouse brain synaptosomes. | | 1977 | 21665 |
| changes in the activity and amounts of enzymes synthesizing catecholamines and acetylcholine in brain, adrenal medulla, and sympathetic ganglia of aged rat and mouse. | | 1977 | 21723 |
| heterogeneity of alkaline ribonuclease in the mouse and ehrlich ascites cells. | the specific activity of alkaline rnase ii was l00 to 1800 times higher in mouse pancreas than in mouse liver, serum, ascites fluid, and ehrlich ascites cell grown intraperitoneally. ehrlich ascites cells grown in cell culture medium had a much lower alkaline rnase ii activity than cells grown intraperitoneally. chromatography on cm-52 cellulose of acid- and heat-treated preparations showned a considerable heterogeneity of the mouse enzymes. depending on the source of the extract, two to six for ... | 1977 | 21728 |
| carfecillin: antibacterial activity in vitro and in vivo. | carfecillin, the alpha-phenyl ester of carbenicillin, hydrolyses rapidly in the presence of serum or body tissues to liberate carbenicillin but hydrolysis is less rapid in aqueous solution. the activity of carfecillin in antibacterial tests in vitro depends upon the extent of hydrolysis to carbenicillin, and in conventional serial dilution tests carfecillin shows an antibacterial spectrum generally similar to that of carbenicillin due to extensive hydrolysis. however, in tests in which the exten ... | 1977 | 21771 |
| synthesis, physicochemical properties, and antitumor activities of analogs of 1-phenyl-3-benzyl-3-methyltriazenes. | to study the effect of substituents on the antitumor activities of analogs of 1-phenyl-3-benzyl-3-methyltriazenes, a series of compounds was designed and synthesized in which the substituent on the 1-phenyl was an electron-withdrawing group and the substituent on the 3-benzyl had a broad range of physicochemical properties. of the 13 analogs prepared and tested against sarcoma-180 in the mouse, five showed significant activity. the results were submitted to discriminant analysis to determine str ... | 1977 | 21950 |
| regulation of the growth and development of sympathetic neurons in vivo. | the superior cervical ganglion (scg) in the neonatal mouse and rat has been employed as a model system to study the regulation of ontogeny of presynaptic cholinergic nerves and postsynaptic adrenergic neurons. during postnatal development presynaptic choline acetyltransferase (chac) activity increases 30- to 40-fold, whereas postsynaptic tyrosine hydroxylase (t-oh) activity rises 6- to 8-fold. transection of the presynaptic cholinergic nerves innervating the scg prevents the normal development o ... | 1977 | 22085 |
| fungal proteases and the mammalian kinin system: i. brinolase-catalyzed kinin formation and s2160 hydrolysis. | brinolase, a fungal protease advocated for thrombolytic therapy, released kinin peptides from semi-purified kininogens of the human, rabbit, guinea pig, and mouse, and moreover cleaved an arginyl bond of the chromogenic peptide s2160. its kinetics demonstrated marked differences from the mammalian protease trypsin. whereas trypsin liberated 100% of the available kinin in 30 min at ph 8, brinolase generated a maximum of only 22% under optimal conditions, viz. incubation of 5 microgram/ml enzyme a ... | 1977 | 22111 |
| translation by bacterial cells of messenger rna for interferon of animal origin. | escherichia coli, strain ab 1157, cells are capable of translating human, mouse, and chicken messenter rna for interferon with production of interferon of the corresponding specifity. this translation occurs in the presence of serum. the activity of the resulting interferon decreased in parallel to dilution of the original mrna preparation, upon multiple ulitization of the mrna solution, as well as upon reduction of the interferon- producing activity of cells-donors of mrna due to prolonged stor ... | 1977 | 22228 |
| massive occurrence of rickettsiae of the spotted fever group in fowl tampan, argas persicus, in the armenian s.s.r. | fowl tampans (argas persicus) collected in oktemberyan in the armenian s.s.r. in 1974 were found to be massively infected with rickettsiae of the spotted fever (sf) group. one isolated rickettsial strain, designated armenia 9, is antigenically related to, and probably identical with, strain armenia 11 and strain b of rickettsia slovaca, isolated from dermacentor marginatus ticks. strain armenia 9 grows readily in chick embryo yolk sacs and in chick embryo cell (cec) and l-cell cultures. it cause ... | 1977 | 22239 |
| effects of dihydroergotoxine mesylate on aging neurons in vitro. | c1300 mouse neuroblastoma cells gradually accumulate lipofuscin-like pigment when they are maintained in culture. pigment was demonstrated by positive straining for acid phosphatase and with periodic acid-schiff stain. pigment was formation in cells was reduced by exposure of the cells to lower doses of dihydroergotoxine mesylate which also induced neurite formation and increased protein synthesis. since lipofuscin appears to originate as a result of wear and tear within the cells, the drug prob ... | 1978 | 22479 |
| effects of culture milieus on the development of mouse blastocysts in vitro. | various culture milieus were examined for their support of mouse blastocyst development. two important variables were the time at which human cord serum was added to the medium and the concentration of amino acids. in the best medium. eagle's minimum essential medium (fortified with six times the usual amino-acid concentration plus 20 percent fetal bovine serum, replaced after 48 hr with human cord serum), 83 percent of the blastocysts shed the zona pellucida, 58 percent developed to the early e ... | 1977 | 22483 |
| demonstration and characterization of a serum factor produced by activated t cells. | spleen rosette forming cells (rfc) from adult thymectomized mice have a low sensitivity to inhibition by anitheta serum (aos) and azathioprine (az) in comparison with normal spleen or thymus rfc. thymus extracts and normal mouse serum (but not spleen extracts or thymectomized mouse serum) correct this abnormality after a 30 min in vitro incubation with spleen cells. we report here the existence of a serum factor produced in allogeneic reactions with the same activity on rosettes as thymic factor ... | 1977 | 22486 |
| arginases of mouse brain and liver. | | 1977 | 22583 |
| a lithium carbonate induced increase in the mouse brain 5-hydroxytryptamine metabolism proceedings. | | 1977 | 22657 |
| anatomical, physiological and biochemical studies of the cerebellum from reeler mutant mouse. | | 1977 | 22882 |
| morphology, histochemistry and isozymes of monkey kidney cells during long-term exposure to cigarette smoke. | monkey kidney cells (vero) in monolayer culture were exposed to 260 puffs of whole smoke from a mid tar range cigarette over a period of 240 days. cell morphology was investigated at the light and electron microscopic level. the histochemical characteristics of several hydrolytic enzymes and dehydrogenases were examined, and the isozymes of ldh, mdh and g6pd studied. for all these parameters smoke exposed cells displayed the same features as sham exposed and negative controls. the cells were ass ... | 1977 | 22975 |
| blockade by psychotropic drugs of the muscarinic acetylcholine receptor in cultured nerve cells. | the ability of antimuscarinics, tricyclic antidepressants, and antipsychotics to block the muscarinic acetylcholine receptor was determined using an assay for this receptor in cultured nerve cells. the technique involved the assay of receptor-mediated formation of guanosine 3',5'-cyclic phosphate (cyclic gmp) from radioactively labeled guanosine 5'-triphosphate in living mouse neuroblastoma cells (clone n1e-115). this cyclic gmp formation occurred rapidly (peak at 30 sec) and was dependent on th ... | 1977 | 23185 |
| alpha-adrenoceptors in the mouse vas deferens and their effects on its response to electrical stimulation. | 1 noradrenaline (id50, 0.75 micrometer) and clonidine (id50, 2.8 nm) produced a dose-related inhibition of the twitch response of the isolated vas deferens of the mouse to electrical stimulation, their effectiveness decreasing as frequency of stimulation increased from 0.2 to 16 hertz. 2 phenylephrine (1.0--2.0 micrometer) produced a dose-related contraction of the mouse isolated vas deferens and potentiated the responses to field stimulation. 3 yohimbine (10 nm) antagonized the inhibitory effec ... | 1978 | 23194 |
| purification of the glutamate decarboxylase from human brain. | a method of purifying the glutamate decarboxylase from human brain is described. the enzyme was purified 8 000 fold in regard to the initial homogenate and appears homogenous by electrophoresis, both in denaturing and non-denaturing conditions. the molecular weight of the native enzyme and its subunits indicate that gad from human brain is formed by two similar if non identical polypeptide chains. the km for glutamate and pyridoxal phosphate found for the human enzyme, respectively 1,2.10(-3) m ... | 1977 | 23221 |
| an in vitro study of functional maturation of murine thymus cells. | critical time of onset of thymus cell functions in ontogeny was studied in vitro. collaborative function in an antibody response and ability to induce a graft-versus-host (gvh) response by murine thymocytes from different stages of ontogeny were investigated. thymocytes from as early as 16-day mouse embryos were capable of collaborating in the antibody response to sheep-erythrocyte-antigen in vitro following 24 h of pretreatment with concanavalin a (con a). by contrast, maturation of thymus cell ... | 1977 | 23333 |
| effects of ph and type of sugar in the medium on tyrosinase activity in cultured melanoma cells. | the tyrosinase (ec 1.14.18.1) activity of cultured mouse melanoma cells b16 in the stationary phase of growth, depends greatly on the ph of the medium and the kind of sugar present. the enzyme activity of a homogenate of cells grown at ph 7.2 in eagles's mem supplemented with 10% new born calf serum and con taining galactose in place of glucose, was about ten times that of a homogenate of cells cultured at ph 6.3 in the same medium. the tyrosinase activity changed reversibly on changing the ph o ... | 1978 | 23384 |
| uptake and metabolism of glutamate in astrocytes cultured from dissociated mouse brain hemispheres. | | 1977 | 23414 |
| an investigation of the ionic mechanism of intracellular ph regulation in mouse soleus muscle fibres. | 1. intracellular ph (ph(i)) of surface fibres of the mouse soleus muscle was measured in vitro by recessed-tip ph-sensitive micro-electrodes. ph(i) was displaced in an acid direction by removal of external (nh(4))(2)so(4) after a short exposure, and the mechanism of recovery from this acidification was investigated.2. removal of external k caused a very slow acidification (probably due to the decreasing na gradient) but had no effect on the rate of ph(i) recovery following acidification. this in ... | 1977 | 23428 |
| identification of transmembrane bridging proteins in the plasma membrane of cultured mouse l cells. | studies were carried out to identify transmembrane bridging proteins in the plasma membrane of mouse l-929 cells. cells grown in suspension culture were 125i-labeled by lactoperoxidase and allowed to ingest latex particles to produce inside-out membrane phagosome preparations. phagosomes were isolated and the inner membrane surface was labeled with n-(5'-aminopentyl)-5-dimethylamino-1-naphthalenesulfonamide (dansylcadavarine) by a transglutaminase-catalyzed reaction. the phagosome membrane prote ... | 1977 | 23534 |
| trypsin-like inhibitor activity in mouse uteri during early gestation and delayed implantation. | | 1978 | 23552 |
| electron microscopic studies of the replication of mouse cytomegalovirus in mouse embryo cells. | during the replication a pathogenic and a non-pathogenic strain of mouse cytomegalovirus in mouse embryo cells, three types of progeny virus particles were observed in both cases; the virus yields of the two strains differed considerably. | 1977 | 23668 |
| an adenosine 3':5'-monophosphate-adenosine binding protein from mouse liver. factors affecting the activation of the binding protein by adenosine 5'-triphosphate. | | 1978 | 23726 |
| investigations of carbromal metabolism (author's transl). | erythro-2-brom-2-ethyl-3-hydroxybutyramide has been isolated from the urines of patients with carbromal intoxications as well as from the urine of rat, mouse and dog treated with either carbromal or with 2-brom-2-ethyl-acetamide. the identification of the excreted and synthetized metabolite was carried out by means of ir, mass and nmr spectroscopy. along with the erythro-2-brom-2-ethyl-3-hydroxybutyramide one other bromine containing compound was isolated after carbromal application only, its ma ... | 1977 | 23742 |
| cytidine 3',5'-monophosphate (cyclic cmp) formation by homogenates of mouse liver. | | 1978 | 23778 |
| synthetic melanocyte stimulating hormone release-inhibiting factor (mif). part iii: effect of l-prolyl-n-methyl-d-leucyl-glycinamide and mif on biogenic amine turnover. | the effect of the factor that inhibits the release of melanocyte stimulating hormone (msh), i.e., l-prolyl-l-leucyl-glycinamide (mif), and l-prolyl-n-methyl-d-leucyl-glycinamide, an analog, on brain norepinephrine (ne), dopamine (da) and serotonin (5-ht) turnover was examined in rats. the analog (40 mg/kg i.p.), in a fashion similar to mif (40 and 5 mg/kg i.p.), increased brain da turnover; only mif (40 mg/kg i.p.) increased endogenous da levels. the analog (40 and 5 mg/kg i.p.) decreased brain ... | 1977 | 23796 |
| purification and characterization of two isozymes of 3-phosphoglycerate kinase from the mouse. | two isozymes of 3-phosphoglycerate kinase (atp:3-phospho-d-glycerate 1-phosphotransferase, ec 2.7.2.3), designated pgk-a and pgk-b, were purified from separate extracts of muscle and testicular tissue of dba/2j mice, respectively. a similar procedure was used to purify the corresponding isozymes from c57bl/6j mice in order to make inter-strain comparisons. the purification involved the use of affinity chromatography with an 8-(6-aminohexyl)amino-atp-sepharose column and deae-sephadex chromatogra ... | 1978 | 23844 |
| studies of cyclic amp action using mutant tissue culture cells. | s49 mouse lymphoma cell mutants, each with a specific defect in its ability to generate or respond to cyclic amp, have been isolated. analysis of the properties of these cells has begun to provide information on complex and significant biologic problems related to the cyclic amp system. | 1978 | 23989 |
| depression of cell-mediated immunity following inoculation of trichinella spiralis extract in the mouse. | mice pretreated with trichinella spiralis extract (tse), or infected with the parasite, rejected primary skin allografts in 18-23 days and secondary allografts in 12-16 days. mice pretreated with saline or with bovine serum albumin (bsa) rejected the primary allografts in 12-18 days and did not accept the secondary grafts. inoculation of increasing doses of parental spleen cells from mice pretreated with saline or with bsa in f1 hybrids produced proportionately stronger graft-versus-host reactio ... | 1978 | 23994 |
| further studies of antisera on the fertilization of mouse, rat, and hamster eggs in vivo and in vitro. | | 1977 | 24006 |
| guinea pig antiserum to mouse cytotoxic t lymphocytes and their precursors. | | 1978 | 24072 |
| the major histocompatibility complex--comparison in the mouse, man, and the rat. a review. | | 1978 | 24344 |
| modification of the immunologic properties of the cell surface. | living cf1 mouse-transplantable spontaneous mammary adenocarcinoma cells were modified with glutaraldehyde, formalin, 2,4,6-trinitrophenylate, vibrio cholerae neurominidase, iodoacetate, heparin, histamine and adenosine 3'5'-monophosphate (camp), then used to immunize syngeneic cf1 mice. animals immunized with the fixed (formalinized or glutaraldehyde fixed) neuraminidase-treated cells or the membrane of these cells, rejected two challenging doses of 10(8) viable unmodified adenocarcinoma cells. ... | 1978 | 24583 |
| the persistent pha-responsive population in the mouse thymus. ii. recirculatory characteristics and immunological properties. | experiments were performed to ascertain whether the persistent pha-responsive cell population found in mouse thymus grafts was capable both of localizing to peripheral lymphoid organs when deliberately injected into suitable recipient mice, and of responding to antigenic stimulation. it was shown that the persistent population could not be found in large numbers in recipient spleens or lymph nodes at late time after transfer. these cells could, however, be found in large numbers in the periphera ... | 1978 | 24584 |
| ther persistent pha-responsive population in the mouse thymus. ii. recirculatory characteristics and immunological properties. | experiments were performed to ascertain whether the persistent pha-responsive cell population found in mouse thymus grafts was capable both of localizing to peripheral lymphoid organs when deliverately injected into suitable recipient mice, and of resonding to antigenic stimulation. it was shown that the persistent population could not be found in large numbers in recipient spleens or lymph nodes at late time after transfer. these cells could, however, be found in large numbers in the peripheral ... | 1977 | 24585 |
| effects of restoring lethally irradiated mice with anti-thy 1.2-treated bone marrow: graft-vs-host, host-vs-graft, and mitogen reactivity. | in vitro treatment of a/j mouse bone marrow with anti-thy 1.2 serum and guinea pig complement (gpc) eliminated its ability to induce graft-vs-host (gvh) mortality in lethally irradiated c57bl/6j x a/f1 (baf1) mice. the anti-thy 1.2 and gpc treatment of a/j marrow significantly reduced spleen cell activation by phytohemagglutinin (pha) but not lipopolysaccharide (lps) stimulation in a/j mice assayed 6 weeks after lethal irradiation and reconstitution with the treated marrow. however, the anti-thy ... | 1978 | 24664 |
| inhibition of mouse brain monoamine oxidase by (+)-amphetamine in vivo. | | 1978 | 24723 |
| oxygen affinity and 2,3-diphosphoglycerate in blood of australian marsupials of differing body size. | oxygen carriage and 2,3-diphosphoglycerate (2,3-dpg) levels have been measured in the blood of seven species of australian marsupials ranging in size from 35 to 0.03 kg. they were red and grey kangaroos, wallaroo, tammar wallaby, brush-tailed possum, potoroo, and brown marsupial mouse. oxygen affinity decreased with decrease in adult body size, standard p50 (at 36 c) varying from 24.6 torr in the largest (red kangaroo) to 41.9 torr in the smallest (brown marsupial mouse). the relationship betwee ... | 1977 | 24866 |
| metabolic, respiratory and haematological adjustments of the little pocket mouse to circadian torpor cycles. | metabolic, respiratory and haematological parameters were investigated for the little pocket mouse during circadian torpor cycles. the rate of o2 consumption decreased from 7.04 to 0.05 ml o2.g-1.hr-1, with a corresponding decrease in respiratory minute volume from 49.4 to 0.9 ml.min-1 during torpor at an ambient temperature of 10 c. no changes in haemoglobin concentration (19.7 g/100 ml), haematocrit (54%), red blood corpuscle count (12.4 10(6)/microliter), mean corpuscular volume (43.6 microme ... | 1977 | 24867 |
| modulation of gvhr and cell-mediated cytotoxicity in the mouse. | | 1978 | 24903 |
| studies on antituberculotic action of some phenothiazine derivatives in vitro. | five phenothiazine derivatives (chlorpromazine, levomepromazine, diethazine, promethazine and chlorpromazine) sulphoxyde were tested for antimycobacterial activity. the growth of mycobacterium tuberculosis, m. bovis and m. butyricum was inhibited by chlorpromazine practically at identical concentrations. the minimum inhibitory concentrations for m. tuberculosis were: chlorpromazine and levomepromazine, 10 microgram/ml; diethazine and promethazine 20 microgram/ml, whilst chlorpromazine sulphoxyde ... | 1977 | 24964 |
| effect of 5-bromo-2'-deoxyuridine on the in vitro dna synthesizing activity of polyoma virus-infected cells. | the in vitro synthesizing activity of uninfected and polyoma virus-infected mouse embryo (me) cells was investigated by measuring the incorporation of 3h-thymidine (dtr) into acidinsoluble material in cell-free extracts. the character of the enzyme reaction was described in uninfected cells and an activating effect of 0.01 m dithiothreitol (dtt) was observed. a variation of the enzyme activity in the course of cultivation of me cells in primary culture was demonstrated. the induction of the dna ... | 1978 | 25003 |
| dengue viral antigens in host cell membranes. | membranes from type 2 dengue virus (den-2) infected bhk cells, when used as immunogen, elicit antibodies detectable by complement fixation, hemagglutination inhibition and plaque reduction neutralization tests with suckling-mouse-brain-derived den-antigens. membrane fractions from infected cells separated by sucrose density gradient centrifugation were employed as antigen in serological tests with anti-den-2 infected suckling mouse brain hyperimmune mouse serum and hyperimmune mouse ascitic flui ... | 1978 | 25005 |
| isolation of tettnang virus from ixodes ricinus ticks in czechoslovakia. | three virus strains closely related to tettnang virus were isolated from nymphs and males of ixodes ricinus tick in moravia and slovakia. the virus isolates were lethal for suckling mice on intracerebral (i.c.) inoculation but the virus titres in the brain were low. the incubation period of 5--12 days in the first mouse passage was shortened to 3 days in further passages. infection of mice was accompanied by ultrastructural changes in the cytoplasm of neurons. groups of spherical virus particles ... | 1978 | 25012 |
| comparative assay of histamine h2-receptor antagonists using the isolated mouse stomach proceedings. | | 1978 | 25107 |
| relation between the drug-metabolizing activities of liver microsomes and multiplicity of cytochrome p-450 (author's transl). | wada et al. found a hyperbolic curve in the lineweaver-burk plots of the drug-metabolizing activity and competitive inhibition of steroid hormones to drugs, and suggested that several enzymes acting on different sites of various steroid hormone molecules might metabolize drugs without having specificities for hydroxylation sites. recently, several cytochrome p-450s were separated and purified from liver microsomes. we studied the relation between the drug-metabolizing activities and multiplicity ... | 1978 | 25234 |
| tyrosine aminotransferase in akr/j albino and c57bl/6j black mouse skin. | l-tyrosine aminotransferase is present in a high speed supernatant fraction of skin homogenate of akr/j albino and c57bl/6j black mice. the conversion of tyrosine to p-hydroxyphenylpyruvate was shown to be catalyzed by an aminotransferase by the following observations: the reaction was partially dependent on the presence of low concentrations of alpha-ketoglutarate; catalase was ineffective in increasing the yield of p-hydroxyphenylpyruvate; there was potent inhibition by typical inhibitors of p ... | 1978 | 25309 |
| tentative identification of benzoapyrene metabolite-nucleoside complexes produced in vitro by mouse liver microsomes. | | 1978 | 25376 |
| inhibition of experimental immediate hypersensitivity reactions by a novel xanthone, ru 31156. | ru 31156, the tris-(hydroxymethyl)-aminomethane salt of 7-(s-methylsulphonimidoyl)-5-(n-hexyl)-xanthen-9-one-2-carboxylic acid has been found to be a potent inhibitor of experimental immediate hypersensitivity reactions in vivo. in the ige-mediated rat pca test, ru 31156 had an ed50 of 0.0046 (00037--0.0057) mg/kg which compared to a figure of 1.21 (1.04--1.42) mg/kg for disocium cromoglycate (dscg), both compounds being administered intravenously. ru 31156 was also active when administered oral ... | 1978 | 25634 |
| pharmacologic responses of cells of a neuroblastoma x glioma hybrid clone and modulation of synapses between hybrid cells and mouse myotubes. | cells of the hybrid clone ng108-15 responded to 5-hydroxytryptamine (5-ht), dopamine or acetylcholine with graded depolarizations involving membrane conductance increases. responses desensitized during continuous application of the neurotransmitters, and responses to 5-ht and dopamine cross-desensitized: a desensitizing application of one neurotransmitter also desensitized the hybrd cell to the other neurotransmitter. 5-ht and acetylcholine did not cross-desensitize. the hybrid cell 5-ht respons ... | 1978 | 25697 |
| identification and properties of the nicotinamide adenine dinucleotide (phosphate)+-dependent malic enzyme in mouse ascites tumor mitochondria. | | 1978 | 25711 |
| toxoplasma antibodies and spontaneous abortion. | one hundred and fifty-two women with spontaneous abortion were investigated by hemagglutination (ha) and immunofluorescence antibody (ifa) tests for toxoplasmosis. in 48 cases, quantitive immunoglobulin (ig) studies and mouse inoculation with gestational material were performed. positive toxoplasma antibody titers were observed in 62 cases (40.8%) using ha and in 52 cases (38.2%) using ifa. this prevalence was significantly higher than that observed in 80 normal women who served as controls. tox ... | 1978 | 25801 |
| study of h-2 mutations in mice. vii. the defectiveness of the h-2ba mutant in the hemagglutination and graft vs host reactions. | three mutants of the h-2k gene within the mouse major histocompatibility complex were studied. antisera anti-h-2.5 + 39 were unable to agglutinate h-2ba and h-2bf mutant red blood cells. the same sera moderately agglutinated h-2b red blood cells. the h-2ba red blood cells did not absorb in vitro the activity from an anti-h-2.5 serum, while the h-2b cells did. thus, these results indicate the existence of qualitative sd antigenic differences among h-2kb derived mutants. in the proliferative graft ... | 1977 | 25825 |
| the development of lysosomal apparatus. i. lysosomal enzyme activities in the liver of mice at perinatal stages and those of their mothers. | the enzymatic activity of five acid hydrolases: acid phosphatase, arylsulfatase a, deoxyribonuclease, beta-glucuronidase, and cathepsin d, was assayed in fetal (fifteenth and eighteenth days of pregnancy) and neonatal (days 0, 5, 10, and 15 post-partum) mouse liver. with the exception of cathepsin d, the activity increased around birth to levels varying according to the enzyme. histochemical observations of other authors appear to justify, at least in part, the present results, which indicate th ... | 1978 | 25903 |
| adrenergic alpha- and beta-receptors expressed by the same cell type in primary culture of perinatal mouse brain. | | 1978 | 25947 |
| regulation of glutamine synthetase in cultured 3t3-l1 cells by insulin, hydrocortisone, and dibutyryl cyclic amp. | the 3t3-l1 mouse fibroblast cell line develops morphological and biochemical characteristics of adipocytes when maintained at confluence. this conversion to adipocytes is accelerated by addition of insulin to the culture medium green, h. & kehinde, o. (1975) cell 5, 19-27. during the course of the insulin-mediated adipocyte conversion, the specific activity (units/mg of protein) of glutamine synthetase l-glutamate:ammonia ligase (adp-forming), ec 6.3.1.2 increases more than 100-fold. the specifi ... | 1978 | 26055 |
| long-term persistence of cell-mediated and humoral immune response in specifically resistant mice given live attenuated langat (e5 "14") virus. | in a long-term experiment, the features of humoral and cell-mediated immunity (cmi) were investigated in mice given intravenously (i.v.) a single dose of live attenuated langat (e5 "14") virus of the tick-borne encephalitis (tbe) complex. the inapparent infection induced in mice specific resistance against challenge with virulent tbe virus, cmi was assayed by the capillary method of murine splenic lymphocyte migrarion inhibition (lmi) in the presence of partially purified tbe vurus. significant ... | 1978 | 26198 |
| 2-br-2-ethyl-4-hydroxybutyramide and 2-br-2-ethyl-4-butyrolactam as carbromal metabolites (author's transl). | 2-brom-2-ethyl-4-hydroxybutyramide and 2-brom-2-ethyl-4-butyrolactam have been isolated from the urine of patients with carbromal intoxications as well as from the urine of rat, mouse and dog after the application of either carbromal or bromdiethylacetamide and identified by means of mass-, nmr- and ir spectroscopy. | 1978 | 26319 |
| molecular studies of subspecificity differences among phosphorylcholine-binding mouse myeloma antibodies using 31p nuclear magnetic resonance. | | 1978 | 26385 |
| mechanism of in vitro mutagenic activation and covalent binding of n-hydroxy-2-acetylaminofluorene in isolated liver cell nuclei from rat and mouse. | | 1978 | 26463 |
| effect of lithium on the membrane-bound magnesium-dependent atpase of mouse neuroblastoma cells. | | 1978 | 26497 |
| lipid-soluble inhibitors of dihydrofolate reductase. i. kinetics, tissue distribution, and extent of metabolism of pyrimethamine, metoprine, and etoprine in the rat, dog, and man. | with the aim of developing anticancer compounds which overcome some of the clinical limitations of the polar dihydrofolate reductase inhibitor, methotrexate, the physicochemical properties, kinetics, and metabolism of a series of lipid-soluble 2,4-diamino-5-phenylpyrimidine folate antagonists have been studied. metoprine and etoprine, potent inhibitors of mammalian dihydrofolate reductase, were compared with pyrimethamine, a widely used antimalarial drug. the development of assay procedures in o ... | 1978 | 26555 |
| subpopulations of mouse t lymphocytes. ii. suppression of graft-vs.-host reactions by naturally proliferating splenic t cells. | the immunological role of a naturally proliferating subpopulation of splenic t cells was investigated using the graft-vs.-host (gvh) reaction on the mouse. normal parental spleen cells, purified splenic t cells or lymph node cells were pulse-treated for one hour in vitro with tritiated thymidine of high specific activity (3hdthd, "thymidine suicide"). the treatment specifically and selectively kills proliferating cells which are actively synthesizing dna, i.e. cells in s phase. following treatme ... | 1978 | 26575 |
| serum polyphenol oxidase activity (ceruloplasmin) in conventional laboratory animals and man. | the optimum ph for ceruloplasmin as polyphenol oxidase (ec 1.10.3.2) activity was determined in human serum (ph 5.4) and the serum of conventional laboratory animals--the rat (ph 5.2), mouse (ph 5.2), hamster (ph 5.3), guinea pig (ph 5.4), multimammate mouse (ph 5.2) and rabbit (ph 5.4). determined at the optimum ph in 0.1m acetate buffer polyphenol oxidase activity fell in the sequence: rat--man--rabbit--mouse--multimammate mouse--hamster--guinea pig. ceruloplasmin polyphenol oxidase activity w ... | 1978 | 26613 |
| interaction of glucocorticoid receptors from lymphoid cell lines with their nuclear acceptor sites. | procedures have been developed which provide simple means of determining binding constants of steroid receptors for glucocorticoids in mouse lymphoid cell lines and of characterizing the interaction of the steroid--receptor complex with the nucleus. an average of 70% of the steroid--receptor complexes is found associated with the nuclear fraction in three investigated cell lines, whereas 30% of the steroid--receptor complexes is found in the cytosol fraction. this distribution of the steroid-rec ... | 1978 | 26620 |
| mice bearing the ob/ob mutation have impaired immunity. | the obese mutant mouse c57bl/6j ob/ob showed impaired ability to reject skin grafts or react to a contact-sensitising agent in comparison with littermate controls (either +/ob or +/+). ability of spleen cells from mice bearing the ob/ob mutation to produce a graft-versus-host reaction in c57bl/6j x dba/2j f1 hybrid mice was not impaired. | 1978 | 26636 |
| quisqualamine, a novel gamma-aminobutyric acid (gaba) related depressant amino acid. | a new substnace, quisqualamine, the decarboxylated analogue of quisqualic acid, predictably depressed electrical activity of neurons of the frog and rat spinal cord in vitro and of the mouse spinal cord in vivo. in the in vitro preparations, the action of quisqualamine was associated with a prolonged depolarization of primary afferent terminals which was sensitive to blockade by picrotoxin and bicuculline and which was also depressed by strychnine. this suggests an interaction of quisqualamine w ... | 1978 | 26767 |
| effects of patulin on the kinetics of substrate and cationic ligand activation of adenosine triphosphatase in mouse brain. | patulin (4-hydroxy-4h-furo3,2-cpyran-2(6h)-one), a carcinogenic lactone produced as a major metabolite by several fungi, inhibited the mg++-dependent na+-k+ activated adenosine triphosphatase (atpase) activity of mouse brain microsomal fractions with an estimated ic50 of 3.0 x 10(-4) m. inhibition was concentration dependent. hydrolysis of atp was linear with both time and enzyme concentration either with or without patulin in reaction mixtures. altered ph and activity curves for na+-k+ atpase d ... | 1978 | 26794 |
| helper-independent transformation by unintegrated harvey sarcoma virus dna. | we have studied the unintegrated infectious dna of harvey sarcoma virus (ha-sv) and moloney leukemia virus (mo-mulv). the source of infectious viral dna was the hirt supernatant fraction from cells acutely infected with ha-sv and mo-mulv. to obtain a direct quantitative assay for infectious viral dna, recipient mouse cells were first exposed to calcium phosphate-precipitated viral dna and then treated with dimethyl sulfoxide. infectivity was monitored by focus formation for ha-sv and xc plaque f ... | 1978 | 26810 |
| tricyclic antidepressants block histamine h1 receptors of mouse neuroblastoma cells. | | 1978 | 26884 |