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[non-indigenous and non-specific parasitic nematodes--the background and the consequences].introduction of the host is the main reason behind introduction of the parasite, e. g., nematodes. the introduction of the japanese eel (anguilla japonica) in europe in the 1980s resulted in expansion of the swimbladder nematode anguillicola crassus which soon invaded not only populations of the european eel (a. anguilla), but also other local fish. the raccoon (procyon lotor) is native to north america. it is the specific host of strongyloides procyonis and baylisascaris procyonis. more than 90 ...200920209803
vaccination strategies for managing brucellosis in yellowstone bison.concerns over migratory bison (bison bison) at yellowstone national park transmitting brucellosis (brucella abortus) to cattle herds on adjacent lands led to proposals for bison vaccination. we developed an individual-based model to evaluate how brucellosis infection might respond under alternate vaccination strategies, including: (1) vaccination of female calves and yearlings captured at the park boundary when bison move outside the primary conservation area; (2) combining boundary vaccination ...201020362620
brucellosis in the united states: role and significance of wildlife reservoirs.regulatory programs for brucellosis in domestic livestock have been active in the u.s. for almost 80 years. wildlife reservoirs of brucellosis include bison (bison bison) and elk (cervus elaphus nelsonii) for brucella abortus whereas brucella suis is the predominant species infecting feral swine. the persistence of brucellosis in wildlife reservoirs poses a risk for reintroduction of brucella into domestic livestock. reducing the prevalence of brucellosis in wildlife reservoirs is anticipated to ...201020362627
characterization of bison (bison bison) myoglobin.bison is an alternate meat species gaining increased popularity in north america. although previous investigations reported that bison meat discolors faster than beef, the molecular basis of this observation has not been investigated. therefore, the objective of the present study was to determine the redox stability, thermostability, and primary structure of bison myoglobin (mb), in comparison with beef mb. purified bison and beef myoglobins were analyzed for autoxidation, lipid oxidation-induce ...201020374756
cd8(+)/perforin(+)/wc1(-) gammadelta t cells, not cd8(+) alphabeta t cells, infiltrate vasculitis lesions of american bison (bison bison) with experimental sheep-associated malignant catarrhal fever.sheep-associated malignant catarrhal fever (sa-mcf) caused by ovine herpesvirus-2 (ovhv-2), a gamma-herpesvirus in the macavirus genus, is a fatal disease associated with lymphoproliferation, lymphocytic vasculitis, and mucosal ulceration in clinically susceptible species. sa-mcf is an important threat to american bison (bison bison) due to their high susceptibility to this disease. currently, the pathogenesis of disease in sa-mcf is poorly understood, and the immunophenotype of lymphocytes that ...201020413164
foraging decisions of bison for rapid energy gains can explain the relative risk to neighboring plants in complex swards.herbivores commonly base their foraging decisions not only on the intrinsic characteristics of plants, but also on the attributes of neighboring species. although herbivores commonly orient their food choices toward the maximization of energy intake, the impact of such choices on neighboring plants remains largely unexplored. we evaluated whether foraging decisions by herbivores aiming at a rapid intake of digestible energy could explain multiple neighboring effects in complex swards. specifical ...201020583724
cattle ancestry in bison: explanations for higher mtdna than autosomal ancestry.understanding and documenting the process of hybridization and introgression between related species is a major focus of recent evolutionary research using molecular techniques. many north american bison herds have cattle ancestry introduced by crossbreeding over a century ago. molecular estimates of this ancestry have shown much higher levels for cattle mtdna than for autosomal cattle genes. a large part of this difference appears to be the result of partial reproductive isolation between the t ...201020637048
status of brucellosis in free-ranging elk and bison in wyoming.brucella abortus is the causative agent of brucellosis, a disease enzootic in populations of free-ranging elk (cervus elaphus) and bison (bison bison) in the greater yellowstone ecosystem, usa. we define the distribution of the disease in elk throughout wyoming, usa, using three epidemiologic/geographic classifications based on winter-foraging opportunity. antibody prevalence for >3,300 yearling and adult, female elk, sampled from supplemental feedgrounds in western wyoming, usa, since 1985, was ...201020688637
in vitro isolation and identification of the first neospora caninum isolate from european bison (bison bonasus bonasus l.).peripheral blood from european bison (bison bonasus bonasus l.) living in białowieża forest, north-east poland, were investigated for the presence of antibodies to neospora caninum and isolation of parasite. out of 23 animals three of them showed a strong positive response to n. caninum (13%). the white blood cells from two positive and two negative bison were loaded on monolayer vero cells culture. the first viable tachyzoites were detected only in positive samples at days 60 and 70 after incub ...201020702042
mycoplasma bovis outbreak in a herd of north american bison (bison bison).a disease outbreak of high morbidity and high mortality in bison (bison bison) was investigated. clinical signs included lameness, swollen joints, respiratory distress, and lethargy. fifty-three of 194 animals died. cows between 5 and 10 years of age were the most affected group, in which 40 of 88 animals died. necropsies were performed on several animals. there were abscesses in the lung and liver, as well as fibrinosuppurative pleuritis, polyarthritis, and disseminated microabscesses in variou ...201020807947
molecular characterization of coprophilous fungal communities reveals sequences related to root-associated fungal endophytes.this paper reports the use of molecular methods to characterize the coprophilous fungal communities (cfc) that inhabit the dung of four species of mammalian herbivores at two sites, sevilleta national wildlife refuge (snwr) in new mexico and wind cave national park (wcnp) in south dakota. results reveal that cfc from domesticated cattle (bos taurus) at snwr, and bison (bison bison) and black-tailed prairie dogs (cynomys ludovicianus) at wcnp were diverse but dominated primarily by members within ...201120842497
complete mitochondrial dna sequence analysis of bison bison and bison-cattle hybrids: function and phylogeny.complete mitochondrial dna (mtdna) genomes from 43 bison and bison-cattle hybrids were sequenced and compared with other bovids. selected animals reflect the historical range and current taxonomic structure of bison. this study identified regions of potential nuclear-mitochondrial incompatibilities in hybrids, provided a complete mtdna phylogenetic tree for this species, and uncovered evidence of bison population substructure. seventeen bison haplotypes defined by 66 polymorphic sites were disco ...201020870040
epizootic hemorrhagic disease outbreak in a captive facility housing white-tailed deer (odocoileus virginianus), bison (bison bison), elk (cervus elaphus), cattle (bos taurus), and goats (capra hircus) in colorado, u.s.a.an ungulate research facility in fort collins, colorado, u.s.a., experienced mortality in white-tailed deer (odocoileus virginianus) because of epizootic hemorrhagic disease virus (ehdv) infection from 20 august 2007 through 26 september 2007. epizootic hemorrhagic disease virus (ehdv) was detected by reverse transcriptase polymerase chain reaction and virus isolation from the spleen and lung tissues of two white-tailed deer. virus neutralization tests were performed on pre- and postoutbreak ser ...201020945651
surgical repair of a cleft palate in an american bison (bison bison).a 4-mo-old bison (bison bison) was evaluated and treated at a university veterinary hospital for a cleft defect in the hard and soft palate. using a mandibular symphysiotomy approach, the palatal defect was repaired with a z-plasty pattern in the soft palate and mucoperiosteal flaps in the hard palate. a small area of dehiscence in the rostral aspect of the hard palate, and aspiration pneumonia, were complications, but the bison calf recovered with medical management. even though this surgical p ...201020945663
some blood biochemical parameter values in a herd of american bison (bison bison l.) from an experimental animal farm in poland.the blood samples of 17 bison (7 cows, a heifer, a young bull and 8 calves) formed the basis for conducting analyses of blood plasma. the measurements were carried out by using ionoselective electrodes aided by a hitachi 917 biochemical analyser. an interpretation of the results was possible due to comparison with the results of similar analyses completed in the united states. many differences in blood chemistry values in bison bred at kurozweki were found in comparison to bison from the us.201020968177
pathological lesions in european bison (bison bonasus) with infestation by ashworthius sidemi (nematoda, trichostrongylidae).asworthius sidemi schulz, 1933 is a blood sucking gastrointestinal nematode, primarily typical for asiatic deer. it was found for the first time in poland in european bison in 1997. to estimate the level of invasion of a. sidemi and histopathological changes connected with the presence of the parasite in the years 2004-2007 parasitological and histopathological examinations of 54 european bison from białowieza forest were carried out. parasitological examination was carried out by the sedimentat ...201021077432
rapid response of a grassland ecosystem to an experimental manipulation of a keystone rodent and domestic livestock.megaherbivores and small burrowing mammals commonly coexist and play important functional roles in grassland ecosystems worldwide. the interactive effects of these two functional groups of herbivores in shaping the structure and function of grassland ecosystems are poorly understood. in north america's central grasslands, domestic cattle (bos taurus) have supplanted bison (bison bison), and now coexist with prairie dogs (cynomys spp.), a keystone burrowing rodent. understanding the ecological re ...201021141180
sperm characteristics in plains (bison bison bison) versus wood (bison bison athabascae) bison.the objective was to compare sperm characteristics between the two subspecies of north american bison, plains bison (bison bison bison) and wood bison (bison bison athabascae). frozen-thawed ejaculated sperm from age-matched plains (n = 3) and wood (n = 2) bison were evaluated for morphometry, motility, viability, protein profile, and in vitro fertilization characteristics. sperm morphometry and motility were assessed with computer-based systems, viability was assessed with sybr-14 and propidium ...201121295823
predicting bison migration out of yellowstone national park using bayesian models.long distance migrations by ungulate species often surpass the boundaries of preservation areas where conflicts with various publics lead to management actions that can threaten populations. we chose the partially migratory bison (bison bison) population in yellowstone national park as an example of integrating science into management policies to better conserve migratory ungulates. approximately 60% of these bison have been exposed to bovine brucellosis and thousands of migrants exiting the par ...201121340035
possibilities of using the european bison (bison bonasus) epididymal spermatozoa collected post-mortem for cryopreservation and artificial insemination: a pilot study.european bison is the largest mammal in europe with the population of approximately 4000 individuals. however, there is no report of post-mortem spermatozoa collection and cryopreservation from this species and the aim of this study was to test if the epididymal spermatozoa collected post-mortem from european bison are suitable for cryopreservation and artificial insemination (ai).201121385402
strontium isotope evidence for migration in late pleistocene rangifer: implications for neanderthal hunting strategies at the middle palaeolithic site of jonzac, france.in order to understand the behaviours and subsistence choices of palaeolithic hunter-gatherers, it is essential to understand the behavioural ecology of their prey. here, we present strontium isotope data from sequentially-sampled enamel from three reindeer (rangifer tarandus ssp.) and a single bison (bison cf. priscus) from the late middle palaeolithic site of jonzac (chez-pinaud), france. the results are used to investigate the ranging and migratory behaviours of these important prey species. ...201121497882
the annual cycle of shedding eimeria oocysts by european bison (bison bonasus) in the bialowieza primeval forest, poland.abstract between april 2008, and march 2009, we analyzed the pattern of coccidian oocysts present in the feces of the european bison (bison bonasus l., 1758) and found 4 species (eimeria bovis, e. canadensis, e. ellipsoidalis, e. zuernii) previously reported from this host and 3 species (eimeria alabamensis, e. cylindrica, e. pellita), which are new host and locality records. all the species occurred in bison females and only 4 in males; e. bovis was the most prevalent in both sexes. the overall ...201121506851
xenografting of testis tissue from bison calf donors into recipient mice as a strategy for salvaging genetic material.the objective was to evaluate the long-term outcome of testis tissue xenografting from neonatal bison calves as a model for closely related rare or endangered ungulates. testis tissue was collected postmortem from two newborn bison calves (bison bison bison) and small fragments of the tissue were grafted under the back skin of immunodeficient recipient mice (n = 15 mice; eight fragments/mouse). single xenograft samples were removed from representative recipient mice every 2 mo after grafting (fo ...201121529912
evaluation of an animal protein-free semen extender for cryopreservation of epididymal sperm from north american bison (bison bison).the objective was to evaluate the suitability of an animal protein-free semen extender for cryopreservation of epididymal sperm from the two subspecies of north american bison: plains (bison bison bison) and wood (bison bison athabascae) bison. both cauda epididymides (from six plains and five wood bison) were minced and incubated in sp-talph buffer for approximately 2 h at 37 °c to release actively motile sperm. sperm suspensions were filtered, centrifuged and the sperm pellet from each bull wa ...201121550101
energy gains predict the distribution of plains bison across populations and ecosystems.developing tools that help predict animal distribution in the face of environmental change is central to understanding ecosystem function, but it remains a significant ecological challenge. we tested whether a single foraging currency could explain bison (bison bison) distribution in dissimilar environments: a largely forested environment in prince albert national park (saskatchewan, canada) and a prairie environment in grasslands national park (saskatchewan, canada). we blended extensive behavi ...201121560694
predicting potential european bison habitat across its former range.habitat loss threatens large mammals worldwide, and their survival will depend on habitat in human-dominated landscapes. conservation planners thus face the challenge to identify areas of least conflict with land use, yet broadscale species distribution models rarely incorporate real landscape patterns nor do they identify potential conservation conflicts. an excellent example of such conservation challenges is provided by european bison (bison bonasus). almost extinct by the early 20th century, ...201121639048
a survey of the fecal bacteria of bison (bison bison) for potential pathogens and antimicrobial susceptibility of bison-origin e. coli.an observational study determined the normal fecal bacterial flora of clinically healthy bison, detected the presence of common potential zoonotic pathogens, and determined the antimicrobial susceptibility of isolated e. coli strains. ninety-six fecal samples from 10 captive herds were cultured for aerobic, anaerobic, facultative, and microaerophillic bacteria. nineteen major genera of gram-positive and 8 genera of gram-negative bacteria were identified. salmonella spp. were not detected but som ...201121731097
exome-wide dna capture and next generation sequencing in domestic and wild species.abstract:201121729323
dna vaccination of bison to brucellar antigens elicits elevated antibody and ifn-+¦ responses.brucella abortus remains a threat to the health and well-being of livestock in states bordering the greater yellowstone area. during the past several years, cohabitation of infected wildlife with cattle has jeopardized the brucellosis-free status of idaho, usa; wyoming, usa; and montana, usa. current livestock b. abortus vaccines have not proven to be efficacious in bison (bison bison) or elk (cervus elaphus nelsoni). one problem with the lack of vaccine efficacy may stem from the failure to und ...201121719815
the influence of elevated temperature conditioning on bison (bison bison bison) meat quality.elevated temperature conditioning (etc: 10°c until 10 h post mortem) was effectively employed as a means of cooling bison carcasses in order to avoid the cold-induced meat quality defects that are a risk with conventional bison carcass chilling (0-2°c for 24 h). the etc treatment maintained internal m. longissimus lumborum and m. semimembranosus temperature above 10°c within the first 10 h post mortem. the time/temperature combination did not result in significant evaporative loss, although loss ...200022062079
blast chilling and low voltage electrical stimulation influences on bison (bison bison bison) meat quality.conventional carcass chilling is a lengthy and energy expensive process. blast chilling (bl) can reduce cooling time and associated shrink loss, although its application may compromise meat quality, particularly in lean carcasses or those with localized finish such as bison. low voltage electrical stimulation (lves) can reduce the risk of decreased meat quality by inducing rapid rigor onset prior to exposure of the musculature to extreme cold temperature. bl (-20°c, 3 m·s(-1) air velocity, 2 h) ...200122061713
electrochemical identification of artificial oligonucleotides related to bovine species. potential for identification of species based on mismatches in the mitochondrial cytochrome c1 oxidase gene.our studies show that electrochemical impedance spectroscopy (eis) and scanning electrochemical microscopy (secm) of films of ds-dna on gold allow us to distinguish between mitochondrial dna fragments of the cytochrome c(1) oxidase (mt-cox1) of three related species of the subfamily 'bovinae' (bos taurus, bison bison, and bison bonasus). in eis, a perfectly matched dna gives rise to a considerably larger charge transfer resistance r(ct) compared to mismatched pairings. differences in charge tran ...201121847503
The efficacy of pour-on ivermectin in bison (Bison bison).To test the effectiveness of pour-on ivermectin in parasitized bison, changes in fecal parasite egg counts after treatment with ivermectin injectable or pour-on preparations were compared to a negative control group. There was no difference between the two ivermectin groups, and both forms of ivermectin were effective in reducing fecal parasite egg counts in parasitized bison.201122043076
quantification of damage at different stages of cryopreservation of endangered north american bison (bison bison) semen and the effects of extender and freeze rate on post-thaw sperm quality.semen cryopreservation is an important technique for the banking of animal germplasm from endangered species and exploitation of genetically superior sires through artificial insemination. being a member of bovidae family, bison semen has poor freezing ability as compared to dairy and beef bulls' semen. this study was designed to quantify the damage to bison sperm at different stages of cryopreservation, and to determine the effects of extender (commercial triladyl(®) vs. custom made tris-citric ...201122240453
toxocara vitulorum in a bison (bison bison) herd from western canada.the discovery of the parasite toxocara vitulorum in bison calves in the province of manitoba, canada is discussed. this parasite is more commonly found in the small intestines of bovid calves living in tropical and subtropical regions of the world. this is the first time that toxocara vitulorum has been reported from hosts in canada.201223277649
immune responses and safety after dart or booster vaccination of bison with brucella abortus strain rb51.one alternative for management of brucellosis in yellowstone national park bison (bison bison) is vaccination of calves and yearlings. although brucella abortus strain rb51 vaccination protects bison against experimental challenge, the effect of booster vaccinations was unknown. this study characterized immunologic responses after dart or booster vaccination of bison with brucella abortus strain rb51. in two studies, 8- to 10-month-old female bison were inoculated with saline (n = 14), hand vacc ...201222461528
farm crops depredation by european bison (bison bonasus) in the vicinity of forest habitats in northeastern poland.european bison, the largest mammal in europe, after being exterminated in the wild and then restored during the 20th century is still listed by the international union for conservation of nature (iucn red list of threatened species) as a species vulnerable to extinction. however, the increasing number of european bison, through creation of new and expansion of existing populations strongly increases the risk of human-bison conflict in the near future. we analyzed the depredation of farm crops by ...201222842710
sequence analysis of bitter taste receptor gene repertoires in different ruminant species.bitter taste has been extensively studied in mammalian species and is associated with sensitivity to toxins and with food choices that avoid dangerous substances in the diet. at the molecular level, bitter compounds are sensed by bitter taste receptor proteins (t2r) present at the surface of taste receptor cells in the gustatory papillae. our work aims at exploring the phylogenetic relationships of t2r gene sequences within different ruminant species. to accomplish this goal, we gathered a colle ...201526061084
first steps into the wild - exploration behavior of european bison after the first reintroduction in western europe.biodiversity is rapidly declining globally. one strategy to help to conserve species is to breed species in captivity and release them into suitable habitats. the way that reintroduced animals explore new habitats and/or disperse from the release site is rarely studied in detail and represents key information for the success of reintroduction projects. the european bison (bison bonasus l. 1758) was the largest surviving herbivore of the post-glacial megafauna in europe before it became extinct i ...201526605549
evidence of low prevalence of mycobacterial lymphadenitis in wild boars (sus scrofa) in poland.mycobacterium spp. and rhodococcus equi are generally regarded as the main causes of lymphadenitis in pigs and wild boars. in poland, mycobacterial submandibular lymphadenitis was first diagnosed in a wild boar in 2012 but mycobacterium spp. infections are also present in the polish population of european bison (bison bonasus). the prevalence of lymphadenitis in polish wild boars has been found to 8.4% (95% ci 6.2-11.3%) and it has been proved that r. equi is not an important cause of purulent l ...201728122588
black-tailed prairie dogs, cattle, and the conservation of north america's arid grasslands.prairie dogs (cynomys spp.) have been eliminated from over 95% of their historic range in large part from direct eradication campaigns to reduce their purported competition with cattle for forage. despite the longstanding importance of this issue to grassland management and conservation, the ecological interactions between cattle and prairie dogs have not been well examined. we address this issue through two complementary experiments to determine if cattle and prairie dogs form a mutualistic gra ...201525760377
hunting the extinct steppe bison (bison priscus) mitochondrial genome in the trois-frères paleolithic painted cave.despite the abundance of fossil remains for the extinct steppe bison (bison priscus), an animal that was painted and engraved in numerous european paleolithic caves, a complete mitochondrial genome sequence has never been obtained for this species. in the present study we collected bone samples from a sector of the trois-frères paleolithic cave (ariège, france) that formerly functioned as a pitfall and was sealed before the end of the pleistocene. screening the dna content of the samples collect ...201526083419
tracking of wisent-bison-yak mitochondrial evolution.one of the most informative sources which allow the drawing of far-reaching conclusions about the origins and phylogenetics of many species, including domestic animals and humans, is mitochondrial dna (mtdna). one of the important research targets should include the identification of similarities between wild and domestic species. the analysis involved the nucleotide sequences of mtdna of wisent, auroch, bison, yak, bovine reference sequence (brs) t3, t3a, t3b, t1, t1a, t1'2'3, t2, t3, t4, t5, q ...201222415349
inferring the phylogeny of bovidae using mitochondrial dna sequences: resolving power of individual genes relative to complete genomes.molecular techniques that assess biodiversity through the analysis of a small segment of mitochondrial genome have been getting wide attention for inferring the mammalian diversity. due to their highly conserved nature, specific mitochondrial genes offer a promising tool for phylogenetic analysis. however, there is no established criteria for selecting the typical mitochondrial dna (mtdna) segments to achieve a greater resolving power. we therefore chose the family bovidae as a model and compare ...201222399841
seasonal shifts in diet and gut microbiota of the american bison (bison bison).north american bison (bison bison) are becoming increasingly important to both grassland management and commercial ranching. however, a lack of quantitative data on their diet constrains conservation efforts and the ability to predict bison effects on grasslands. in particular, we know little about the seasonality of the bison diet, the degree to which bison supplement their diet with eudicots, and how changes in diet influence gut microbial communities, all of which play important roles in ungu ...201526562019
mitochondrial genome analysis reveals historical lineages in yellowstone bison.yellowstone national park is home to one of the only plains bison populations that have continuously existed on their present landscape since prehistoric times without evidence of domestic cattle introgression. previous studies characterized the relatively high levels of nuclear genetic diversity in these bison, but little is known about their mitochondrial haplotype diversity. this study assessed mitochondrial genomes from 25 randomly selected yellowstone bison and found 10 different mitochondr ...201627880780
novel graphical analyses of runs of homozygosity among species and livestock breeds.runs of homozygosity (roh), uninterrupted stretches of homozygous genotypes resulting from parents transmitting identical haplotypes to their offspring, have emerged as informative genome-wide estimates of autozygosity (inbreeding). we used genomic profiles based on 698 k single nucleotide polymorphisms (snps) from nine breeds of domestic cattle (bos taurus) and the european bison (bison bonasus) to investigate how roh distributions can be compared within and among species. we focused on two len ...201627872841
early cave art and ancient dna record the origin of european bison.the two living species of bison (european and american) are among the few terrestrial megafauna to have survived the late pleistocene extinctions. despite the extensive bovid fossil record in eurasia, the evolutionary history of the european bison (or wisent, bison bonasus) before the holocene (<11.7 thousand years ago (kya)) remains a mystery. we use complete ancient mitochondrial genomes and genome-wide nuclear dna surveys to reveal that the wisent is the product of hybridization between the e ...201627754477
lack of effects of ooplasm transfer on early development of interspecies somatic cell nuclear transfer bison embryos.successful development of iscnt (interspecies somatic cell nuclear transfer) embryos depends on complex interactions between ooplasmic and nuclear components, which can be compromised by genetic divergence. transfer of ooplasm matching the genetic background of the somatic cell in iscnt embryos is a valuable tool to study the degree of incompatibilities between nuclear and ooplasmic components. this study investigated the effects of ooplasm transfer (ot) on cattle (bos taurus) and plains bison ( ...201627737629
lessons for livestock genomics from genome and transcriptome sequencing in cattle and other mammals.decreasing sequencing costs and development of new protocols for characterizing global methylation, gene expression patterns and regulatory regions have stimulated the generation of large livestock datasets. here, we discuss experiences in the analysis of whole-genome and transcriptome sequence data.201627534529
foraging decisions underlying restricted space use: effects of fire and forage maturation on large herbivore nutrient uptake.recent models suggest that herbivores optimize nutrient intake by selecting patches of low to intermediate vegetation biomass. we assessed the application of this hypothesis to plains bison (bison bison) in an experimental grassland managed with fire by estimating daily rates of nutrient intake in relation to grass biomass and by measuring patch selection in experimental watersheds in which grass biomass was manipulated by prescribed burning. digestible crude protein content of grass declined li ...201627547359
a survey to detect toxocara vitulorum and other gastrointestinal parasites in bison (bison bison) herds from manitoba and saskatchewan.an egg count survey using environmental fecal samples obtained in spring or early summer was conducted to estimate the apparent prevalence of toxocara vitulorum in unweaned bison calves and of other intestinal parasites in adult bison on 98 farms in manitoba and saskatchewan. calf samples were pooled (maximum 5 samples per pool) by farm and positive pools were examined to determine individual t. vitulorum counts. toxocara vitulorum eggs were found on 4 farms in manitoba and none in saskatchewan. ...025183895
cross-reactivity of neutralizing antibodies among malignant catarrhal fever viruses.some members of the gamma herpesvirus genus macavirus are maintained in nature as subclinical infections in well-adapted ungulate hosts. transmission of these viruses to poorly adapted hosts, such as american bison and cattle, can result in the frequently fatal disease malignant catarrhal fever (mcf). based on phylogenetic analysis, the mcf viruses (mcfv) cluster into two subgroups corresponding to the reservoir hosts' subfamilies: alcelaphinae/hippotraginae and caprinae. antibody cross-reactivi ...201526658281
cross-sectional study of schmallenberg virus seroprevalence in wild ruminants in poland at the end of the vector season of 2013.in view of recurrent schmallenberg virus (sbv) infections all over europe between 2011 and 2013, a lively scientific debate over the importance of the sylvatic transmission cycle of the virus has emerged. the study presents results of serosurvey which included wild ruminants representing species of red deer (cervus elaphus), roe deer (capreolus capreolus), european bison (bison bonasus), fallow deer (dama dama), mouflon (ovis orientalis musimon) hunted or immobilized at 34 different locations of ...201425528665
malignant catarrhal fever in american bison (bison bison) experimentally infected with alcelaphine herpesvirus 2.malignant catarrhal fever (mcf), due to ovine herpesvirus 2 (ovhv-2), causes appreciable death loss in ranched bison (bison bison) throughout north america. no vaccine exists to protect animals from disease. since ovhv-2 has not been propagated in vitro, one strategy to develop a modified live vaccine is to use a closely related, non-pathogenic member of the malignant catarrhal fever virus family as a vector expressing potentially protective ovhv-2 epitopes. to date, no controlled experimental c ...201424803260
the infection of reintroduced ruminants - bison bonasus and alces alces - with anaplasma phagocytophilum in northern poland.the north-eastern part of poland is considered an area of high risk for infection with tick-borne diseases, including with human granulocytic ehrlichiosis (hge) agents. the etiological agent of hge is anaplasma phagocytophilum. as the animal reservoir for a. phagocytophilum in the environment serve the species from cervidae and bovidae families. european bison (bison bonasus) and elk (alces alces) are the big ruminant species, reintroduced to the forests of middle europe after many decades of ab ...201526408585
the occurrence of anaplasma phagocyto- philum in wild bison from the bialowieza primeval forest in eastern poland.the aim of the present study was to investigate the occurrence of anaplasma spp. in a group of 120 wild bison (bison bonasus) from the bialowieza primeval forest in eastern poland and to determine which species of anaplasma could infect these animals based on a pcr of a part of the 16s rrna gene followed by sequencing. the pcr technique showed the presence of 16s rrna anaplasma spp. genetic material in the blood of 22 from a total of 120 animals. dna amplification by means of the primers ehr 521 ...201526281444
influence of management and biological factors on the parasitic invasions in the wild-spread of blood-sucking nematode ashworthius sidemi in european bison (bison bonasus).the full course of new parasite introductions in wild animals is difficult to accurately trace. we documented and analysed the invasive blood-sucking nematode ashworthius sidemi (trichostrongylidae) introduction and spread in european bison (bison bonasus) from the initial phase of its progression. in the polish part of the białowieża primeval forest (bpf) the parasite was first found in 2000. from 2002 to 2015, 165 culled bison were investigated. the prevalence and intensity of a. sidemi schulz ...201627761404
the parasitic fauna of the european bison (bison bonasus) (linnaeus, 1758) and their impact on the conservation. part 1. the summarising list of parasites noted.during the current century, 88 species of parasites have been recorded in bison bonasus. these are 22 species of protozoa (trypanosoma wrublewskii, t. theileri, giardia sp., sarcocystis cruzi, s. hirsuta, s. hominis, s. fusiformis, neospora caninum, toxoplasma gondii, cryptosporidium sp., eimeria cylindrica, e. subspherica, e. bovis, e. zuernii, e. canadensis, e. ellipsoidalis, e. alabamensis, e. bukidnonensis, e. auburnensis, e. pellita, e. brasiliensis, babesia divergens), 4 trematodes species ...201425119348
descriptive epidemiology of detected anthrax outbreaks in wild wood bison (bison bison athabascae) in northern canada, 1962-2008.we inventoried and assessed historical anthrax outbreak data from 1962-2008 in wild wood bison (bison bison athabascae) in wood buffalo national park and the slave river lowlands (srl), northwest territories, canada. we compared these results with a 2010 outbreak in the srl. anthrax outbreaks have occurred in 12 of the years between 1962 and 2008 in wild wood bison with 1,515 anthrax deaths detected. the average number of carcasses found each outbreak year was 126 (range 1-363), though local ave ...201424779457
dances with anthrax: wolves (canis lupus) kill anthrax bacteremic plains bison (bison bison bison) in southwestern montana.bacillus anthracis, the cause of anthrax, was recovered from two plains bison (bison bison bison) cows killed by wolves (canis lupus) in montana, usa, without associated wolf mortality in july 2010. this bison herd experienced an epizootic in summer 2008, killing ∼ 8% of the herd, the first documented in the region in several decades. no wolf deaths were associated with the 2008 event. surveillance has continued since 2008, with research, ranch, and wildlife personnel diligent during summer. as ...201424484485
genetic diversity among bacillus anthracis soil isolates at fine geographic scales.environmental samples were collected from carcass sites during and after anthrax outbreaks in 2000 and 2001 in the bison (bison bison) population within wood buffalo national park and the hook lake region north of wood buffalo national park. bacillus anthracis spores were isolated from these samples and confirmed using phenotypic characterization and real-time pcr. confirmed b. anthracis isolates were typed using multiple-locus variable-number tandem repeat analysis (mlva15) and single-nucleotid ...201222773624
serological evidence of besnoitia spp. infection in canadian wild ruminants and strong cross-reaction between besnoitia besnoiti and besnoitia tarandi.bovine besnoitiosis, caused by besnoitia besnoiti, is considered to be emergent in europe and responsible for severe economic losses due to the chronic and debilitating course of the disease but has not been reported in north america. besnoitia tarandi is a related species and it has been reported in reindeer and caribou from different locations of the arctic pole, including north america. diagnosis of clinical besnoitiosis is largely based on the recognition of dermal grossly visible tissue cys ...201222795672
serological study of exposure to selected arthropod-borne pathogens in european bison (bison bonasus) in poland.bison bonasus is an indigenous species of central and eastern europe with the largest wild population inhabiting białowieża primeval forest; however, free-living and captive european bison are reared in many countries around the world. despite that the european bison was rescued from the extinction after the first world war, it remains as endangered species. changing environment as well as human activity may have contributed to the observed increase of the risk of the emergence and re-emergence ...201627291825
detection of borrelia burgdorferi sensu lato dna in the blood of wild bison from białowieza primeval forest in eastern poland.the aim of the present study was to investigate the occurrence of borrelia burgdorferi sensu lato dna in a group of 120 wild bison (bison bonasus) from the bialowieza primeval forest in eastern poland. the pcr technique revealed the presence of 16s rna of borrelia burgdorferi sensu lato in the blood of 16 out of 120 examined animals. dna amplification by means of primers sc1 and sc2 gave a product with a size of 300-bp. the sequences of the pcr products obtained showed 100% homology with each ot ...201425638986
brucellosis transmission between wildlife and livestock in the greater yellowstone ecosystem: inferences from dna genotyping.the wildlife of the greater yellowstone ecosystem carries brucellosis, which was first introduced to the area by cattle in the 19th century. brucellosis transmission between wildlife and livestock has been difficult to study due to challenges in culturing the causative agent, brucella abortus . we examined b. abortus transmission between american bison ( bison bison ), rocky mountain elk ( cervus elaphus nelsoni), and cattle ( bos taurus ) using variable number tandem repeat (vntr) markers on dn ...201728118557
risk assessment and management of brucellosis in the southern greater yellowstone area (ii): cost-benefit analysis of reducing elk brucellosis prevalence.recent cases of bovine brucellosis (brucella abortus) in cattle (bos taurus) and domestic bison (bison bison) of the southern greater yellowstone area (sgya) have been traced back to free-ranging elk (cervus elaphus). several management activities have been implemented to reduce brucellosis seroprevalence in elk, including test-and-slaughter, low-density feeding at elk winter feedgrounds, and elk vaccination. it is unclear which of these activities are most cost-effective at reducing the risk of ...201627836044
feasibility of quarantine procedures for bison (bison bison) calves from yellowstone national park for conservation of brucellosis-free bison.objective--to determine the feasibility of qualifying individuals or groups of yellowstone national park bison as free from brucellosis. design--cohort study. sample--serum, blood, and various samples from live bison and tissues taken at necropsy from 214 bison over 7 years. procedures--blood was collected from bison every 30 to 45 days for serologic tests and microbiological culture of blood for brucella abortus. seropositive bison were euthanized until all remaining bison had 2 consecutive neg ...201424548234
the use of contraception as a disease management tool in wildlife.contraception offers potential as a tool for managing certain diseases in wildlife, most notably venereally transmitted diseases or diseases transmitted at parturition. brucellosis is an excellent example of an infectious disease present in wild populations that could potentially be managed through immunocontraception. previous studies in bison (bison bison) suggest that a single injection of gonacon (national wildlife research center, u.s. department of agriculture/animal and plant health inspe ...201324437094
integrating ecology with management to control wildlife brucellosis.bison (bison bison) and elk (cervus elaphus) in the greater yellowstone ecosystem have long been infected with brucella abortus. the continued culling of large numbers of yellowstone bison to reduce the risk of brucellosis transmission to cattle could negatively affect long-term conservation. a desirable management objective is to reduce the level of b. abortus infection while conserving wildlife populations. identifying the ecological factors that influence immune suppression and vulnerabilityt ...201323837381
risks of brucella abortus spillover in the greater yellowstone area.recurrent spillover of brucella abortus from wildlife reservoirs to domestic cattle in the greater yellowstone area (gya) has prevented the united states from completely eradicating bovine brucellosis. risks to cattle are a function of the size and location of wildlife and livestock populations, the degree and nature of spatio-temporal interactions between the various hosts, the level of disease in wildlife, and the susceptibility of livestock herds. while the brucellosis prevalence in wild, fre ...201323837366
evaluation of bison (bison bison) semen from yellowstone national park, montana, usa, bulls for brucella abortus shedding.to determine if bison (bison bison) bulls from yellowstone national park (ynp), montana, usa, shed an infective dose of brucella abortus in semen, 50 ynp bulls were captured on public lands in montana during the winter and early spring (april-may) of 2010 and 2011. the bulls were immobilized, and blood and semen samples were collected for serology and brucella culture. thirty-five bulls (70%) were antibody-positive, and b. abortus was cultured from semen in three (9%) of the 35 antibody-positive ...201323778628
evaluation of transmission of brucella abortus strain 19 in bison by intravaginal, intrauterine, and intraconjunctival inoculation.bovine brucellosis, caused by the bacterium brucella abortus, is endemic in bison (bison bison) and elk (cervus elaphus nelsoni) populations in the area of yellowstone national park, usa. two strategies have been proposed to reduce the risk of transmission of disease in bison: remote vaccination with the vaccine rb51, and the use of immunocontraception of bison to decrease shedding of organisms from infected females. the frequent occurrence of venereal transmission in bison would complicate eith ...201323778600
yersinia enterocolitica: an unlikely cause of positive brucellosis tests in greater yellowstone ecosystem bison (bison bison).yersinia enterocolitica serotype o:9 has identical o-antigens to those of brucella abortus and has apparently caused false-positive reactions in numerous brucellosis serologic tests in elk (cervus canadensis) from southwest montana. we investigated whether a similar phenomenon was occurring in brucellosis antibody-positive bison (bison bison) using y. enterocolitica culturing techniques and multiplex pcr of four diagnostic loci. feces from 53 yellowstone bison culled from the population and 113 ...201222740518
anti-brucella antibodies in moose (alces alces gigas), muskoxen (ovibos moschatus), and plains bison (bison bison bison) in alaska, usa.we used an indirect enzyme-linked immunosorbent assay (ielisa) and the rose bengal test (rbt) to test for anti-brucella antibodies in moose (alces alces gigas), muskoxen (ovibos moschatus), and plains bison (bison bison bison) from various game management units (gmus) in alaska, us, sampled from 1982 to 2010. a portion of the sera had previously been tested with the standard plate test (spt), the buffered brucella antigen (bba) card test, and the card test (card). no antibody-positive plains bis ...201626540335
development of a multiplex pcr for identification of dictyocaulus lungworms in domestic and wild ruminants.dictyocaulus lungworms are the causative agents of parasitic bronchitis (dictyocaulosis) characterised by coughing and severe lung pathology in domestic and wild ruminants. the objective of this study was to design a simple molecular test that could detect of lungworm dna from both adult and larval lungworms and could distinguish between the most common dictyocaulus species found in cattle and in some species of wild ruminants. a multiplex pcr test with four novel primers targeting species-speci ...201526266883
molecular analysis of lungworms from european bison (bison bonasus) on the basis of small subunit ribosomal rna gene (ssu).dictyocaulosis (nematoda: trichostrongyloidea) is a widespread parasitosis of the european bison (bison bonasus) inhabiting bialowieza primeval forest. bearing in mind the current coexistence of bison with wild cervids, and with domestic ruminants in the 19th and 20th century, the need arose for molecular identification of lungworm species. molecular analysis was done on adult lungworms that were obtained from the respiratory track of four free-roaming bison euthanized as a part of the populatio ...201424570058
coccidia (apicomplexa: eimeriidae) of the lowland european bison bison bonasus bonasus (l.).coprological studies conducted between 2007 and 2011 in free-roaming and captive european bison bison bonasus (linnaeus, 1758) from poland revealed 11 species of eimeria infecting the host, i.e., eimeria alabamensis, eimeria auburnensis, eimeria bovis, eimeria brasiliensis, eimeria bukidnonensis, eimeria canadensis, eimeria cylindrica, eimeria ellipsoidalis, eimeria pellita, eimeria subspherica, and eimeria zuernii. the typical host for all isolated species is cattle. the most prevalent species ...201424702772
observations on sporulation of eimeria bovis (apicomplexa: eimeriidae) from the european bison bison bonasus: effect of temperature and potassium dichromate solution.the present study was designed to determine the influence of temperature and the addition of potassium dichromate solution (k2cr2o7) on the efficiency of sporulation of eimeria bovis (zublin, 1908), a coccidian species most commonly diagnosed in european bison bison bonasus (linnaeus). sporulation under conventional conditions (2.5% dichromate solution, 23 °c) was completed on the fourth day of incubation (control), whereas the oocyst development took two days more at the temperature of 18 °c, a ...201525960564
mitochondrial dna haplotype analysis of liver fluke in bison from bialowieza primaeval forest indicates domestic cattle as the likely source of infection.we have determined the mitochondrial genotype of liver fluke present in bison (bison bonasus) from the herd maintained in the bialowieza national park in order to determine the origin of the infection. our results demonstrated that the infrapopulations present in the bison were genetically diverse and were likely to have been derived from the population present in local cattle. from a consideration of the genetic structure of the liver fluke infrapopulations we conclude that the provision of hay ...201322925823
hepatitis e virus antibody prevalence in wildlife in poland.hepatitis e is an important public health problem mostly in developing but occasionally also in industrialized countries. domestic and wildlife animals are considered reservoirs of the hepatitis e virus (hev). since no information on the prevalence of autochthonous hev infections in human and animal in poland is available, the aim of the study was to investigate the hev seroprevalence of different wildlife species as potential virus reservoirs in the country. no hev antibodies were found in any ...201524655475
histophilosis as a natural disease.histophilus somni is responsible for sporadic disease worldwide in cattle and, to a lesser extent, in small ruminants, bighorn sheep (ovis canadensis), and north american bison (bison bison). the importance of h. somni diseases can be attributed to improved clinical and laboratory recognition, combined with the growth in intensive management practices for cattle. although outbreaks of bovine histophilosis can occur year-round, in northern and southern hemispheres, it is most frequent in late fal ...201626847357
serological studies to determine the occurrence of johne's disease and mycoplasma infection in the northern-east polish population of european bison (bison bonasus).a serological study of twenty three european bison (bison bonasus) derived from northern-east poland for the seroprevalence of mycobacterium avium subsp. paratuberculosis, mycoplasma bovis, mycoplasma mycoides subsp. mycoides sc, mycoplasma agalactiae and mycoplasma capricolum subsp. capripneumoniae was conducted. only specific antibodies to m. bovis were detected in two animals (8.7%) which were connected with the clinical signs and macroscopic anatomopathological lesions.201425638988
contrasting results of culture-dependent and molecular analyses of mycobacterium avium subsp. paratuberculosis from wood bison.reduced to near extinction in the late 1800s, a number of wood bison populations (bison bison athabascae) have been re-established through reintroduction initiatives. although an invaluable tool for conservation, translocation of animals can spread infectious agents to new areas or expose animals to pathogens in their new environment. mycobacterium avium subsp. paratuberculosis, a bacterium that causes chronic enteritis in ruminants, is among the pathogens of potential concern for wood bison man ...201323686265
the modification and evaluation of an elisa test for the surveillance of mycobacterium avium subsp. paratuberculosis infection in wild ruminants.enzyme-linked immunosorbent assay (elisa) is often used to test wildlife samples for mycobacterium avium subsp. paratuberculosis (map) infection. however, commercially available kits are only validated for use with domestic ruminant species. a literature review was performed to document the current use of map serum elisa in wild and semi-domestic ruminants. we then modified and evaluated a commercial elisa kit (idexx mycobacterium paratuberculosis antibody test kit) for use with species for whic ...201323302439
mycobacterium bovis in a european bison (bison bonasus) raises concerns about tuberculosis in brazilian captive wildlife populations: a case report.tuberculosis caused by mycobacterium bovis is an important worldwide zoonosis and has been reported to cause clinical disease in several animal species, including captive wildlife. this report describes a case of m. bovis infection in a european bison from a brazilian zoo and compiles a number of literature reports that raise concern regarding tuberculosis among captive wildlife in brazil.201728187776
bayesian analysis to evaluate tests for the detection of mycobacterium bovis infection in free-ranging wild bison (bison bison athabascae) in the absence of a gold standard.we estimated the sensitivity and specificity of the caudal-fold skin test (cft), the fluorescent polarization assay (fpa), and the rapid lateral-flow test (rt) for the detection of mycobacterium bovis in free-ranging wild wood bison (bison bison athabascae), in the absence of a gold standard, by using bayesian analysis, and then used those estimates to forecast the performance of a pairwise combination of tests in parallel. in 1998-99, 212 wood bison from wood buffalo national park (canada) were ...201525973619
[mycobacterium infection in prehistoric humans: co-evolution in remote ages].the introduction of agriculture and animal husbandry at the end of the mesolithic era, despite enabling a significant demographic growth through an increase in food storage and availability, caused new infectious noxae to enter the pathocoenosis. however in the palaeolithic era, hunter-gatherers were already in contact with infectious diseases of animal origin, albeit episodically. modern biomedical technologies allow us to estimate, with better approximation, how long mankind has been in contac ...201525819058
risk factors for mycoplasma bovis-associated disease in farmed bison (bison bison) herds in western canada: a case-control study.north american bison producers have been attempting to control and prevent mycoplasma bovis-associated disease without the benefit of bison-specific knowledge. the objective of this study was to determine the clinical presentation of disease associated with m. bovis infection in western canadian farmed bison, and to identify herd-level risk factors for m. bovis-associated disease. bison producers (n=49) from western canada (manitoba, saskatchewan, alberta, and british columbia) were selected for ...201627317324
mycoplasma bovis isolates recovered from cattle and bison (bison bison) show differential in vitro effects on pbmc proliferation, alveolar macrophage apoptosis and invasion of epithelial and immune cells.in the last few years, several outbreaks of pneumonia, systemically disseminated infection, and high mortality associated with mycoplasma bovis (m. bovis) in north american bison (bison bison) have been reported in alberta, manitoba, saskatchewan, nebraska, new mexico, montana, north dakota, and kansas. m. bovis causes chronic pneumonia and polyarthritis syndrome (cpps) in young, stressed calves in intensively-managed feedlots. m. bovis is not classified as a primary pathogen in cattle, but in b ...201627016754
in vitro antimicrobial susceptibility of mycoplasma bovis clinical isolates recovered from bison (bison bison).mycoplasma bovis is a pathogen globally affecting cattle and bison herds, causing pneumonia, arthritis, mastitis, abortions, and other symptoms, leading to huge economic losses. many studies have been done regarding the antimicrobial susceptibility of m. bovis isolated from cattle, but no such study is available for isolates recovered from bison. for the first time, in vitro susceptibilities of 40 m. bovis clinical isolates collected from bison herds in canada are reported here. minimal inhibito ...201626854525
a serological and molecular study on the occurrence of mycoplasmas in european bison (bison bonasus) from two areas of eastern poland.european bison (bison bonasus) from two different areas of eastern poland showing gross pathology possibly associated with mycoplasma infections were tested for ruminant mycoplasma species using serological and molecular methods. fifty-five samples, blood or tissue were collected from 28 animals during 2013-2014. six sera were positive for mycoplasma bovis. the elisa and complement fixation test for mycoplasma mycoides subsp. mycoides gave a few weak reactions, but were negative by immunoblottin ...201526812834
systemic mycoplasmosis with dystocia and abortion in a north american bison (bison bison) herd.the current study describes a fatal mycoplasma bovis infection in a north american bison (bison bison) cow and her aborted fetus in a herd suffering unusual mortality associated with dystocia and abortion. postmortem evaluation of the subject case found severe caseonecrotic bronchopneumonia, chronic fibrinous pleuritis and pulmonary sequestra, foci of caseous necrosis in the kidneys, and necrotizing endometritis and placentitis. histologic findings in the maternal tissues include endometrial and ...201323847228
necrotic pharyngitis associated with mycoplasma bovis infections in american bison (bison bison).mycoplasma bovis has emerged as a significant and costly infectious disease problem in bison, generally presenting as severe, caseonecrotic pneumonia. three diagnostic cases in which m. bovis is associated with necrotic pharyngitis in bison are described in the current study. the bacterium was isolated from lesions of the pharynx or lung of 3 american bison (bison bison), at 2 different locations in the upper midwestern united states, with severe, necrotic pharyngeal abscesses. chronic caseonecr ...201323512925
estimating neospora caninum prevalence in wildlife populations using bayesian inference.prevalence of disease in wildlife populations, which is necessary for developing disease models and conducting epidemiologic analyses, is often understudied. laboratory tests used to screen for diseases in wildlife populations often are validated only for domestic animals. consequently, the use of these tests for wildlife populations may lead to inaccurate estimates of disease prevalence. we demonstrate the use of bayesian latent class analysis (lca) in determining the specificity and sensitivit ...201627099713
malignant catarrhal fever: an emerging disease in the african buffalo (syncerus caffer).within the tribe bovini in the subfamily bovinae, the water buffalo (bubalus bubalis), american bison (bison bison), european bison (bubalus bonasus) and yak (bos grunniens) are recognized as species highly susceptible to malignant catarrhal fever (mcf). in contrast, the lack of reports describing clinical mcf in the african buffalo (syncerus caffer) whether free ranging or captive has led to a perception that african buffaloes are resistant to mcf. during the last decade, several cases of mcf i ...201523957274
fibroblasts express ovhv-2 capsid protein in vasculitis lesions of american bison (bison bison) with experimental sheep-associated malignant catarrhal fever.american bison (bison bison) are particularly susceptible to developing fatal sheep-associated malignant catarrhal fever (sa-mcf) caused by ovine herpesvirus-2 (ovhv-2), a γ-herpesvirus in the macavirus genus. this generally fatal disease is characterized by lymphoproliferation, vasculitis, and mucosal ulceration in american bison, domestic cattle (bos taurus), and other clinically susceptible species which are considered non-adapted, dead-end hosts. the pathogenesis and cellular tropism of ovhv ...201323953727
ovine herpesvirus 2 infection in american bison: virus and host dynamics in the development of sheep-associated malignant catarrhal fever.ovine herpesvirus 2 (ovhv-2) is a gammaherpesvirus that causes sheep-associated malignant catarrhal fever (sa-mcf), a frequently fatal disease mainly of ruminants. this study was designed to define virus-host dynamics following experimental ovhv-2 infection in bison. a transient peak in viral dna accompanied by the presence of ovhv-2 orf25, orf50 and orf73 transcripts was observed in lungs only from 9 to 12 days post-inoculation (dpi), suggesting occurrence of viral replication. this initial vir ...201222592216
sarcocystis cruzi infection in wood bison (bison bison athabascae).endangered wood bison (bison bison athabascae) is the largest terrestrial mammal in the american continent. animal health is an important issue in their conservation, and sarcocystis cruzi may be a cause of clinical disease in bovidae. hearts of eight wood bison from alaska, usa were examined for sarcocysts by histology, transmission electron microscopy, pepsin digestion, and molecularly. sarcocystis bradyzoites were found in pepsin digests of all eight and sarcocysts were found in histologic se ...201525868849
the first detection of the tick-borne encephalitis virus (tbev) rna in dermacentor reticulatus ticks collected from the lowland european bison (bison bonasus bonasus l.).tick borne encephalitis virus (tbev) (flaviviridae, flavivirus) is the causative agent of tick-borne encephalitis (tbe), a potentially fatal neurological infection. the disease is endemic in a large region in eurasia, where is transmitted mainly by hard ticks: ixodes ricinus and i. persulcatus. it is known that also dermacentor reticulatus is involved in a circulation of tbev, but the knowledge of its importance in the tbe epidemiology is still insufficient. the białowieża primeval forest is loc ...201626751883
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