| cardiac myosin from pig heart ventricle. purification and enzymatic properties. | a method is described for the preparation of high purity myosin from the left ventricle of pig heart. the purified myosin was free from nucleic acid, actin, tropomyosin, troponin, the 150,000 molecular weight protein and other contaminants. analyses of subunits in the purified myosin were carried out on 3.5% acrylamide gel with 0.1% sds. of the total protein present in myosin, 11.3% was in the light chains; light chain 1 (lc1), 5.9% and light chain 2 (lc2), 5.4%. urea gel electrophoresis of the ... | 1976 | 10292 |
| partial purification and characterization of a triglyceride lipase from pig adipose tissue. | a triglyceride lipase was extracted from defatted pig adipose tissue powder with dilute ammonia and purified about 230-fold by a combination of ammonium sulfate fractionation, heparin-sepharose 4b, deae-cellulose, and sephadex g-150 column chromatographies and isoelectrofocusing electrophoresis. the enzyme was distinguishable in physical and kinetic properties from the two previously defined lipases in adipose tissue, lipoprotein lipase, and hormone-sensitive lipase. the purified enzyme was full ... | 1976 | 10295 |
| the occurrence of salmonellas, mycobacteria and pathogenic strains of escherichia coli in pig slurry. | ninety-eight samples of pig slurry from 54 farms were examined for the presence of salmonellas, porcine enteropathogenic strains of haemolytic escherichia coli and mycobacteria. salmonellas were isolated from 12 farms (22%) and enteropathogenic e. coli from 13 farms (24%). pathogenic mycobacteria were not isolated. salmonellas were isolated from 7 of 16 farms (44%) stocked with 'minimal disease' pigs compared with only 5 of 38 farms (13%) stocked with conventionally reared pigs. conversely enter ... | 1976 | 10332 |
| the pharmacology and therapeutic use of antihistamines. | | 1976 | 10337 |
| thiamine-modifying properties of fish and meat products. | | 1976 | 10361 |
| pharmacological studies with adenine, adenosine and some phosphorylated derivatives on guinea-pig tracheal muscle. | adenine, adenosine and three adenine nucleotides all caused relaxation of the guinea-pig trachea. the relaxation to the nucleotides was often preceded by a contraction. the response to adenosine and the nucleotides, but not adenine, was potentiated by dipyridamole. imidazole inhibited the response to adenine alone. propranolol has no effect on the response to any of the compounds. it is concluded that the guinea-pig trachea does not possess and a nucleotide-specific receptor as has been postulat ... | 1976 | 10367 |
| identification of new in vivo side-chain acid metabolites of delta1-tetrahydrocannabinol. | | 1976 | 10374 |
| assessment of two in vitro methods for determination of the apparent affinities of beta-sympathomimetics on myocardial contractility. | | 1976 | 10380 |
| a benzimidazole derivative (7110 md) with gastric antisecretory and antiulcer activity. | | 1976 | 10381 |
| chemical stimulants of shaking behaviour. | | 1976 | 10386 |
| influence of various substances on prostaglandin biosynthesis by guinea-pig chopped lung. | guinea-pig chopped lung tissue was used to investigate the inhibitory effect of various steroidal and non-steroidal anti-inflammatory agents, antipyretics, analgesics, local anaesthetics and psychotropic drugs on mechanically induced release of prostaglandin-like material. low concentrations of non-steroidal anti-inflammatory agents inhibited synthesis, but other antipyretics and analgesics, and the local anaesthetics had little effect. thymoleptics, neuroleptics and monoamine oxidase inhibitors ... | 1976 | 10390 |
| metabolic n-oxidation of atropine, hyoscine and the corresponding nor-alkaloids by guinea-pig liver microsomal preparations. | incubation of guinea-pig liver microsomal preparations with atropine or hyoscine resulted in the formation of the corresponding nor-alkaloids and both isomers of atropine n-oxide from atropine and one isomer of hyoscine n-oxide from hyoscine. separate incubations of guinea-pig liver microsomal preparations with nor-atropine and nor-hyoscine yielded the corresponding hydroxylamines. the n-oxide and hydroxylamine metabolites were identified by comparison of their t.l.c. behavior and m.x. with prep ... | 1976 | 10391 |
| the microsomal n-oxidation of phentermine. | the microsomal n-oxidation of phentermine (ia) to n-hydroxyphentermine (ib) and to alpha,alpha-dimethyl-alpha-nitroso-beta-phenylethane (ic was investigated. maximum activities were obtained with microsomal (9000 g supernatant and microsomes) fractions of rabbit liver in the presence of an nadph generating system. incubation of ia with hepatic washed microsomes from a phenobarbitone pretreated rabbit increased the formation of ib and decreased that of ic but the total amount of n-oxidized metabo ... | 1976 | 10392 |
| a new class of irreversible muscarinic antagonists: beta-haloethylamine furoates. | the synthesis of a class of ultra-long acting muscarinic antagonists is described. furthermore, it is noted that the stability of the, in situ, aziridinium ion is sufficiently great so that these agents can be used to study the effect of temperature upon the conformation of the muscarinic cholinoceptor. the inactivation kinetics of these receptors as well as the dissociation constants, kd, for all probes are presented. | 1976 | 10393 |
| meso-dimercaptosuccinic acid a chelating agent for the treatment of mercury and lead poisoning. | | 1976 | 10397 |
| variations of the enzyme inducing effects of contraceptive agents in different animal species. | | 1976 | 10399 |
| the enolization of 4,5-dehydromuscarone. | | 1976 | 10405 |
| loperamide binding to opiate receptor sites of brain and myenteric plexus. | loperamide, a new antidiarrheal agent, was tested to determine whether its biological activity involves binding to opiate receptor sites. loperamide and morphine competitively inhibited 3h-naloxone binding to homogenates a guinea-pig brain and myenteric plexus. the kp values obtain in the presence of na+ were: morphine, 9.60-10(-9) m (brain), 1.66-10(-7) m (myenteric plexus); loperamide, 7.20-10(-9) m (brain), 1.33-10(-7) m (myenteric plexus); naloxone, 4.78-10(-10) m (brain), 1.27-10(-9) m (mye ... | 1976 | 10422 |
| isoproterenol antagonism of cardioselective beta adrenergic receptor blocking agents: a comparative study of human and guinea-pig cardiac and bronchial beta adrenergic receptors. | pa2 values against isoproterenol were determined for a number of cardioselective and noncardioselective beta adrenergic receptor blocking agents using human and guinea-pig isolated atrial and bronchial or tracheal preparations to study possible species differences. no significant differences in pa2 values for propranolol, pindolol, ro 3-4787, acebutolol, atenolol, practolol, metoprolol, h 87/07 and tolamolol on bronchial or tracheal beta adrenergic receptors of both species were found. with resp ... | 1976 | 10427 |
| studies on the mode of antagonism between adrenergic beta-mimetics and beta-blocking agents (iii). functional antagonism between beta-mimetics and spasmogens. | new equations which can explain the following characteristic phenomena in the functional antagonism between isoproterenol (iso) and spasmogens in guinea-pig trachea are proposed: (1) the amplitude of relaxation of the muscle induced by iso varied depending on the concentration of a spasmogen used (histamine or carbachol). (2) the dose-response curves for the relaxation by iso shifted to the right in a parallel manner as the concentration of the spasmogen increased, and became stationary at high ... | 1976 | 10457 |
| the physiological neurotransmitter with morphine-like activity. | | 1976 | 10557 |
| sympathomimetic bronchodilator drugs. | | 1976 | 10580 |
| uptake, release and metabolism of drugs in the lungs. | | 1976 | 10582 |
| the göttinger minipig as a laboratory animal (author's transl). | blood pictures, serumelectrolytes, acid-base- and bloodgas-values of the arterial blood were determined from two juvenile and one adulte collectives of minipigs (gottingen strain), which were kept under spf-conditions. the results were compared with the corresponding values of human blood and should be regarded as base- or orienting values for further investigations. | 1976 | 10610 |
| mechanism of 2-naphthylamine oxidation catalysed by pig liver microsomes. | 1. in pig liver microsomes 2-naphthylamine-dependent nadph oxidation, oxygen reduction, and hydroxylamine formation are linear with time for several minutes. a sharp increase in nadph oxidation and oxygen uptake then coincides with an abrupt loss of hydroxylamine from the medium. 2. the initial rate of 2-naphthylamine n-oxidation correlates with the microsomal concentration of mixed-function amine oxidase and the extent of linear accumulation of hydroxylamine is dependent on microsomal nadph-cyt ... | 1976 | 10687 |
| technics for the experimental study of delayed hypersensitivity. | the author reviews the principal experimental methods proposed for the study of the sensitising power of medications. all these methods involve delayed hypersensitivity (type iv) and fall into the same broad category, with a sensitisation phase, a rest phase and a stimulating phase. a critical review of these various phases is made; together with the interpretation of the results and their significance. | 1976 | 10786 |
| cardiopulmonary effects of the combination of neuroleptic azaperone and hypnotic metomidate in swine. | cardiopulmonary measurements were made at given intervals up to 120 minutes on 6 awake, unanesthetized pigs given azaperone and metomidate. decreases from control values occurred in arterial blood pressure (deltabpart = 30 mm of hg), heart rate (deltahr = 30 to 35 beats/minute), and cardiac index (deltaci = 1.5 l/minute/m2). blood gas and ph measurements indicated no severe impairment of pulmonary function or arterial acidosis. although the drugs led to decreases in the various functions, cardio ... | 1976 | 10822 |
| influence of adrenaline, dibenamine and dopamine on acidosis, hemoconcentration and lethality in protracted anaphylactic shock of guinea-pigs. | protracted anaphylactic shock of guinea-pigs was accompanied by a marked decrease in blood ph, and an increase in hematocrit. death ensued in 58.3% of the animals within 3 hr of observation. infusion of adrenaline (20 mug/kg/min), after eliciting anaphylaxis, intensified the acidosis, and increased the lethality to 100%. pretreatment with dibenamine (5 mg/kg) reversed the effect of adrenaline. dopamine, infused in amounts of 200 mug/kg/min, acted similarly to the combination dibenamine/adrenalin ... | 1976 | 10865 |
| the activity and properties of adenosine triphosphatase in various swine organs (liver, cerebral and kidney cortex, small intestinal mucosa). | studies into the activity of adenosine triphosphatase (atpase) in homogenates of liver, cerebral cortex, renal cortex, and mucosa of small intestine of swine have shown differentiated activity patterns, with peak activity developing in the liver. this has been related to a particularly high metabolism performance of the liver in fattening pigs. no difference was found to exist between magnesium activation of atpase of swine tissue homogenates and that in tissue obtained from ruminants. atpase wh ... | 1976 | 10870 |
| circulation load test and quality of meat in fattening pigs of known genetic origin. | 102 fattened pigs with known genetic origin were tested on a conveyor-belt 1--4 days before slaughtering. 64 animals run in the first passage in each case 154 m in a 4 minutes (0.64 m/s), compared to it the 38 animals of the second passage run in each case 770 m in 20 minutes. measurements of heart frequency and breathing and body temperature were taken before and at intervals until 30 minutes after movement. after slaughtering the values of ph, colour and drip loss of the m. longissimus dorsi w ... | 1976 | 10871 |
| physico-chemical properties of swine vesicular disease virus. | titration of svdv on primary pig kidney cell cultures revealed a plating efficiency of less than or equal to 0,9 x 10(-3). concentration and purification of the svd-virus propagated on pig kidney cell cultures were done by chloroform treatment, adsorption, differential- and density gradient centrifugation. the following physical parameters were found: svdv is an isometrical rna-virus having a diameter of 25,1 +/- 1,0 nm. it is resistent to the action of chloroform, ether and ph. the virus has a ... | 1976 | 10872 |
| the rotation aeration system (system fuchs) for the treatment of liquid animal and municipal wastes. 5. destruction of ascaris eggs in swine slurry and municipal sewage. | | 1976 | 10881 |
| determination of the mechanism and kinetic constants for hog kidney gamma-glutamyltransferase. | the initial-velocity kinetics of hog kidney gamma-glutamyltransferase were studied. glutamate gamma-(4-nitroanilide) and its 3-carboxy derivative, glutamate gamma-(3-carboxy-4-nitroanilide), served as gamma-glutamyl donors, and glycylglycine as an acceptor. reaction products were identified by paper chromatography and amino acid analysis. inhibited ping pong mechanisms and a comprehensive initial- velocity expression were developed which account for the observed simultaneous gamma-glutamyl trans ... | 1976 | 10887 |
| properties of particulate, membrane-associated and soluble guanylate cyclase from cardiac muscle, skeletal muscle, cerebral cortex and liver. | 1. guanylate cyclase of washed particles and plasma membranes showed s-shaped progress curves when titrated with either gtp or mn2+ ions; similar results were obtained with triton x-100-solubilized enzyme preparation from washed particles. hill plots of these data revealed multiple metal-nucleotide and free-metal binding sites. 2. guanylate cyclase of supernatant fractions displayed typical michaelis-menten properties when enzyme required excess of (free) mn2+ (over gtp) for maximal activities; ... | 1976 | 10891 |
| adenylate cyclase, guanylate cyclase and cyclic nucleotide phosphodiesterases of guinea-pig cardiac sarcolemma. | 1. the activities of the enzymes involved in the metabolism of cyclic nucleotides were studied in sarcolemma prepared front guinea-pig heart ventricle; the enzyme activities reported here were linear under the assay conditions. 2. adenylate cyclase was maximally activated by 3mm-naf; naf increased the km for atp (from 0.042 to 0.19 mm) but decreased the ka for mg2+ (from 2.33 to 0.9 mm). in the presence of saturating mg2+ (15 mm), mn2+ enhanced adenylate cyclase, whereas co2+ was inhibitory. bet ... | 1976 | 10895 |
| energized gastric microsomal membrane vesicles--an index using metachromatic dyes. | | 1976 | 10914 |
| catalase activates cerebral granylate cyclase in the presence of sodium azide. | | 1976 | 10917 |
| reactions of beta-fluoroalanine and beta-bromolanine with d-amino acid oxidase. | | 1976 | 10918 |
| non-specificity of sulphydryl inhibition of the alpha adrenergic response. | | 1976 | 10921 |
| metabolism of lorazepam. | the metabolism of lorazepam by man and four other species is reviewed. lorazepam and its metabolites in blood, urine and faeces were identified by thin-layer and gas chromatography and by mass spectrometry. the principal metabolite in man, dog, pig and cat is the glucuronide, but the rat produces other metabolites after small doses of lorazepam, and significant amounts of the glucuronide only after high doses. since all metabolites, except the glucuronide, occur in small quantities only in man, ... | 1976 | 10938 |
| a review of the animal pharmacology of labetalol, a combined alpha- and beta-adrenoceptor-blocking drug. | 1 the animal pharmacology of labetalol, a drug that blocks both alpha- and beta-adrenoreceptors, is reviewed. 2 in isolated tissues the blockade by labetalol of both alpha- and beta-adrenoreceptors satisfied accepted critera for competitive antagonism. in contrast phentolamine was a competitive antagonist at alpha-adrenoreceptors only, and propranolol a competitive antagonist at beta-adrenoreceptors only. labetalol was 6-10 times less potent than phentolamine in blocking alpha-adrenoreceptors an ... | 1976 | 10948 |
| solubilization, partial purification and radioassay for the intrinsic factor receptor from the ileal mucosa. | a macromolecule which binds intrinsic factor saturated with vitamin b12 has been solubilized from the guinea-pig ileum by homogenization followed by mechanical disruption without organic solvents or detergents. this intrinsic factor 'receptor' was further purified by precipitation with 30% saturated ammonium sulphate, centrifugation at 105000 g, and filtration through sephadex g-200. failure to precipitate the receptor following centrifugation at 105000 g for 3 h and filtration of the receptor w ... | 1976 | 10957 |
| studies on the role of methionine in porcine pancreatic phospholipase a2. | the unique methionine-15 residue located at the n-terminal site of iso- or beta-phospholipase a2 from porcine pancrease has been specifically carboxymethylated with iodoacetic acid. the modification results in a complete inactivation of the enzymatic activity toward micellar and monomeric substrates. spectroscopic measurements reveled that the carboxymethylated protein still binds ca2+ and monomeric substrates with comparable affinities as the native enzyeme. the active site histidine-54 residue ... | 1976 | 10991 |
| the effect of calcium ions on the hydrolysis of benzoylarginine ethyl ester by porcine enteropeptidase. | calcium ions are shown to have a marked ph-dependent effect on the kinetics of benzoyllarginine ethyl ester hydrolysis by porcine enteropeptidase (ec 3.4.21.9). below ph 6.0, calcium ions stimulate benzoylarginine ethyl ester hydrolysis but inhibit this activity above ph 6.0. this effect is mainly on the km for benzoylarginine ethyl ester. at ph 5.3, 2mm calcium ions reduce the km for benzoylarginine ethyl ester from 0.31 mm to 0.26 mm while at ph 6.5 the km increases four-fold from 0.035 mm to ... | 1976 | 10992 |
| investigations on the kinetic properties of estrone glucuronyltransferase from pig kidney. | the microsomal fraction of the pig kidney catalyzes the glucuronidation of estrone in the presence of udp-glucuronic acid. this bireactant system exhibits a sequential type of reaction mechanism. increasing concentrations of either substrate increase the affinity of the enzyme for the other substrate. the hill coefficient, n, was calculated to be 1.0 for both estrone and udp-glucuronic acid. the kestrone and kudp-glucuronic acid are 6.6 mum and 254 mum, respectively. the estrone glucuronyltransf ... | 1976 | 10996 |
| calcium incorporation by smooth muscle microsomes. | the purpose of the present work was to study the factors influencing calcium incorporation into a microsomal fraction prepared from the longitudinal smooth muscle of the guinea-pig ileum. calcium incorporation required the presence of both atp and mg2+ and was unaffected by azide. it was enhanced by oxalate; this effect was ph dependent and it was maximal at ph 6.6. the relation between calcium uptake with oxalate and free ca2+ concentration in the medium was represented by a curve with an optim ... | 1976 | 11001 |
| some complementary data on ah 5158, an inhibitor of both alpha-and beta-adrenoceptors proceedings. | | 1976 | 11020 |
| biometric determination of pheromone production in pigs. | | 1976 | 11139 |
| detection of multiple molecular forms of the gamma-glutamyltransferase by concanavalin a affinity chromatography (author's transl). | the separation of several forms of gamma-glutamyl-transferase was achieved by using concanavalin a-sepharose columns. the enzyme of the adult liver was bound totally to the lectin, whereas only 5% of the kidney enzyme and 50% of the pancreas gamma-glutamyltransferase was adsorbed by concanavalin a. due to a higher content of n-acetylneuraminic acid, the enzyme of the fetal liver does not show any affinity to concanavalin a. within 8 days after birth the n-acetylneuraminic acid-rich fetal gamma-g ... | 1976 | 11165 |
| slow reacting substance as a preformed mediator from human lung. | homogenates from human lung contained a preformed slow reacting substance (psrs). the pattern of contraction on the guinea-pig ileum by psrs was indistinguishable from that of srs-a. the activity of psrs could not be attributed to the presence of k+, na+, ca2+ and mg2+ ions, or any prostaglandin including pgf2 or its 15-oxo derivative. as with srs-a, psrs could be absorbed onto amberlite xad-2 and silicic acid. both were eluted from the former with 80 per cent ethanol and from the latter with a ... | 1976 | 11180 |
| mechanism of intramolecular activation of pepsinogen. evidence for an intermediate delta and the involvement of the active site of pepsin in the intramolecular activation of pepsinogen. | intramolecular pepsinogen activation is inhibited either by pepstatin, a potent pepsin inhibitor, or by purified globin from hemoglobin, a good pepsin substrate. also, pepsinogen at ph 2 can be bound to a pepstatin-sepharose column and recovered as native zymogen upon elution in ph 8 buffer. kinetic studies of the globin inhibition of pepsinogen activation show that globin binds to a pepsinogen intermediate. this interaction gives rise to competitive inhibition of intramolecular pepsinogen activ ... | 1976 | 11216 |
| 6-phosphogluconate dehydrogenase. purification and kinetics. | a method is described for the isolation and purification of 6-phosphogluconate dehydrogenase from pig liver. the molecular weight is estimated at 83,000 and that of the subunits is 42,000 as determined by gel electrophoresis. the ph maximum is 8.5 in 50 mm glycine/naoh buffer and from 7.5 to 10 in 50 mm phosphate buffer at 30 degrees. magnesium ion is not required for activity and acts as an inhibitor at concentrations above 20 mm. a cellular fractionation study indicates that this enzyme is loc ... | 1976 | 11218 |
| brush border motility. microvillar contraction in triton-treated brush borders isolated from intestinal epithelium. | the brush border of intestinal epithelial cells consists of an array of tightly packed microvilli. within each microvillus is a bundle of 20-30 actin filaments. the basal ends of the filament bundles are embedded in and interconected by a filamentous meshwork, the terminal web, which lies directly beneath the microvilli. when calcium and atp are added to isolated brush borders that have been treated with the detergent, triton x-100, the microvillar filament bundles rapidly retract into and throu ... | 1976 | 11222 |
| pharmacological differences of non-adrenergic inhibitory response and of atp-induced relaxation in guineq-pig tracheal strip-chains. | | 1976 | 11303 |
| effects of animal maturity on smooth muscle and blood pressure responses to prostaglandins e2 and f2alpha. | the effects of prostaglandins e2 (pge2) and f2alpha (pgf2-alpha) on muscle strips from mature and immature rats and guinea-pigs and on rat blood pressure were investigated in the presence of atropine. the colon and stomach strips from immature rats were equally responsive to pge 2 and pgf2alpha where mature colons were significantly more sensitive to pgf2alpha and mature stomach strips significantly more sensitive to pge2. on the ileum from immature guinea-pigs the maximum responses to pge2 and ... | 1976 | 11308 |
| actions of the muscle relaxant chandonium iodide on guinea-pig ileum and vas deferens preparations. | the effects of the newly synthesized neuromuscular blocking agent, chandonium iodide (17a-methyl-3beta-pyrrolidino-17a-aza-d-homo-5-androstene dimethiodide) have been investigated on guinea-pig isolated ileum and vas deferens preparations. on the ileum, chandonium (0-1-10-0 mug ml(-1); 1-6 x 10(-7) m-1-6 x 10(-5) m) had weak muscarinic receptor blocking action (pa2 is 5-7), but no antihistamine properties at the concentration tested. no evidence for anticholinesterase actions was found. on the v ... | 1976 | 11309 |
| effect of synthetic motilin and related polypeptides on contraction of gastrointestinal smooth muscle. | | 1976 | 11319 |
| structure-activity relationships of methionine-enkephalin. | | 1976 | 11324 |
| biotransformation of drugs: quantitative structure-activity relationships for barbiturates, tertiary amines, and substituted imidazoles. | when using free energy-related physicochemical parameters, stimulation of nadph oxidation by barbiturates and the n-oxidation of tertiary amines was found to be primarily dependent upon the lipophilic character of the substrates as measured by log p, where p is the partition coefficient from either 1-octanol-water or corn oil-water solvent systems. in contrast, the inhibition of epoxidation of aldrin by a series of substituted imidazoles appears to be much more dependent on electronic (sigma) an ... | 1976 | 11333 |
| absorption, utilization, and safety of aspartic acid. | the dicarboxylic amino acids, asparate and glutamate, occupy unique positions in intermediary metabolism, particularly in the mitochondria, where they play important roles in nitrogen and energy metabolism. administration of large quantities of glutamate and asparate to the newborn mouse produces a variety of neurotoxic effects, the most marked of which is neuronal necrosis. neurotoxic effects of glutamate and aspartate in animal species other than the rodent are highly controversial. in the mos ... | 1976 | 11355 |
| a role of cathepsin b1 in polymorphonuclear leukocytes chemotaxis. | | 1976 | 11383 |
| on the latency and form of the membrane responses of smooth muscle to the iontophoretic application of acetylcholine or carbachol. | | 1976 | 11484 |
| affinity chromatography of 15-hydroxyprostaglandin dehydrogenases from swine kidney. | | 1976 | 11651 |
| the opposite temperature-sensitivity character (ts) in two attenuated flaviviruses, used for human immunization: 17d yellow fever and e5"14" (langat) viruses. a reappraisal of thoughts. | for the reproduction of the man-attenuated e5"14" clone of the langat virus (tick-borne encephalitis complex) in pig kidney epithelial cells, the temperature of 39 degrees c was shown as restrictive, whereas it was permissive for the 17d strain of yellow fever virus and three virulent strains of tick-borne encephalitis (western subtype) virus. the temperature of 36 degrees c permitted the reproduction of all viruses studied. the implication of genetic marker studies in the assessment of human ne ... | 1976 | 11663 |
| a new water-soluble substrate for the determination of trypsin activity. | | 1976 | 11705 |
| structure-activity relationship studies of derivatives of aminotetralins and open chain analogs in relation to beta and alpha-adrenoceptor agonist activities. | some derivatives of aminotetralins and their open chain analogs were tested for their ability to alter blood pressure and heart rate in anesthetized cats with both vagi nerves sectioned and their ability to relax tracheal smooth muscle of guinea-pigs. tl-257 and jod-213, tertiary amines, produced weak alpha-adrenoceptor stimulating activity while jod-176 and jod-211, secondary amines, showed beta-adrenoceptor stimulating activity. another compounds, m-8, an aminotetralin that is a secondary amin ... | 1976 | 11757 |
| appetite stimulant activity of 3-carboxy-10,11-dihydrocyproheptadine. | the orexigenic and ancillary pharmacologic properties of 3-carboxy-10,11-dihydrocyproheptadine (cdc) were compared to those of cyproheptadine. the threshold dose, 0.0312 mg/kg p.o., of cdc for increasing food intake in the cat is similar to that of cyproheptadine, but cdc has a broader effective dose range, extending to 8 mg/kg p.o., compared with 1 mg/kg p.o. for cyproheptadine. using an increase in food consumption of 20% or more as the criterion of a positive response, the dose effective in 5 ... | 1976 | 11758 |
| competitive binding of iron by transferrins from different vertebrates. | 1. a competitive dialysis technique has been used to study the relative affinities of the two iron-binding sites on transferrin molecules and the relative binding strengths of transferrins isolated from plasma of different species. 2. the comparisons were extended to include desialylated human transferrin, ovotransferrin, and a cyanogen bromide fragment of the latter. 3. although the results of bilateral experiments could generally be accounted for in terms of the theory of independent sites, th ... | 1976 | 11826 |
| the effect of propranolol on cold-induced thermogenesis in the newborn pig. | | 1977 | 11955 |
| intraretinal acid-base studies using ph glass microelectrodes: effect of respiratory and metabolic acidosis and alkalosis on inner-retinal ph. | | 1976 | 12001 |
| role of a histidine residue in the active site of cyclic amp-dependent histone kinase. | | 1976 | 12004 |
| physicochemical properties of heparin, and its interaction with cu(ii) and calcium in relation to anticoagulation. | | 1977 | 12019 |
| the degradation of porcine neurophysin i by alkaline conditions. | | 1976 | 12033 |
| improved alkylation of acetoacetyl-coenzyme a thiolase by extension of chain length in chloromethyl ketone fatty acids. | | 1976 | 12041 |
| the interaction of clotting factor x with adsorbents containing bivalent cations. | | 1976 | 12051 |
| studies on phospholipase a in trimeresurus flaoviridis venom. iii. purification and some properties of phospholipase a inhibitor in habu serum. | phospholipase a ec 3.1.1.4 inhibitor was purified from habu (trimeresurus flavivurudls) serum by gel filtration on sephadex g-200, chromatography on de-23 cellulose and affinity chromatography on a sepharose 4b-phospholipase a column. by these procedures, a 31-fold increase in specific activity was attained with a yield of 15%. the purified material was homogeneous as judged by cellulose acetate and polyacrylamide gel electrophoresis. it had an apparent molecular weight of 100,000 as measured by ... | 1976 | 12144 |
| interaction between d-amino acid oxidase and small molecules. | 1. from the standpoint of monomer-dimer equilibrium of hog kidney d-amino acid oxidase ec 1.4.3.3 and the interaction between the enzyme and small molecules, the effect of ph on the binding of p-aminobenzoate to the monomer and dimer of the enzyme was studied by kinetic methods and spectrophotometric titration. 2. the maximum binding number of p-aminobenzoate to the dimer is two molecules, and there is no interaction between the two active sites of the dimer (i.e., no cooperativity) over the ran ... | 1976 | 12150 |
| a study of the interaction between d-amino acid oxidase and quasi-substrates. | to study the interaction between d-amino acid oxidase ec 1.4.3.3 and quasi-substrates such as benzoate and o-, m-, and p-aminobenzoate, visible circular dichroism spectra (cd spectra) were measured and the binding rate and affinity of o-aminobenzoate to the enzyme were observed by following the absorption changes at various wavelengths. we found a new cd band around 560 nm, corresponding to the charge-transfer complexes which result from the formation of aminobenzoate complexes with the enzyme. ... | 1976 | 12151 |
| investigation of microsomal oxygenases of biosynthetic processes. stearyl-coa desaturase of adipose tissue and liver. | porcine and rat microsomal stearyl-coa desaturases require reduced pyridine nucleotide and oxygen, are cyanide sensitive, and are insensitive to carbon monoxide. the km for stearyl-coa is somewhat larger for liver than for the adipose desaturases, but, in general, assay conditions are quite similar. adipose tissue microsomes contain cytochromes b5 and p-450, as well as the nadh- and nadph-specific cytochrome reductases. compared to liver, the specific contents and activities of electron carriers ... | 1976 | 12166 |
| a nonelectrogenic h+ pump in plasma membranes of hog stomach. | differential and density gradient centrifugation were used to prepare a vesicular membrane fraction from hog gastric mucosa enriched 17-fold with respect to cation-activated atpase and 5'-ampase. fractionation of the gradient material by free flow electrophoresis resulted in a fraction 35-fold enriched in cation-activated atpase and essentially free of 5'-ampase and mg2+atpase. the addition of atp to either fraction resulted in h+ uptake and rb+ efflux. the ionophoric and osmotic sensitivity sho ... | 1976 | 12175 |
| 6,8-di-t-butyl-4-oxo-4h-1-benzopyran-2-carboxylic acid: a chromone derivative with anti-allergic, anti-inflammatory and uricosuric activity. | | 1976 | 12270 |
| the concentration of basic and acidic compounds in guinea-pig lung proceedings. | | 1976 | 12364 |
| selective alpha-adrenoceptor blocking actions of a new derivative of 2-halogenotheylamine: 6-(2-bromoethyl)-10,11-methylenedioxy-5,6,7,8-tetrahydrodibenzc,eazocine. | a new compound, 6-(2-bromoethyl)-10,11-methylenedioxy-5,6,7,8-tetrahydrodibenz c,e azocine (da-viii-mbr) was found to have a more selective alpha-adrenergic blocking action than dibenamine or phenoxybenzamine. from dose-response curves for adrenaline and 5-hydroxytryptamine (5-ht) obtained in strips of rat aorta before and after incubation with each of the three blocking agents, the fractions of receptors remaining active for adrenaline and 5-ht, respectively, were estimated. after blockade with ... | 1976 | 12395 |
| the ecology and medical importance of the tahyna virus (author's transl). | in 1958 a virus transmitted by a mosquito was isolated in the slovakian village of tahyna. since this virus was unknown in europe, a detailed study was begun. it was shown that this virus belonged to the california group of arboviruses and occurs in practically all european countries. it could very well be that young hares are the hosts for this virus, and that it multiplies in them. foals and suckling pigs are also hosts and possibly also increase the virus. in patients observed so far, infecti ... | 1976 | 12467 |
| opiate receptors in the brain. | | 1977 | 12470 |
| restoration by histamine of the calcium-dependent electrical and mechanical response in the guinea-pig papillary muscle partially depolarized by potassium. | electrophysiological and mechanical effects of histamine were observed in guinea-pig papillary muscle which had been depolarized and rendered inexcitable by elevation of potassium concentration in tyrode solution to 27 mm. 1. histamine (3 x 10(-7) to 3 x 10(-5) m) restored the action potential and tension development. the amplitude of the action potential was increased by 31.6 mv/10-fold increase in extracellular ca2+ concentration. nifedipine (10(-6) m) abolished the electrical and mechanical r ... | 1976 | 12480 |
| the action of a toxin from the sea anemone anemonia sulcata upon mammalian heart muscles. | the cardiac activity of toxin ii, a basic polypeptide (m.w.: 4770) from the sea anemone anemonia sulcata, was investigated in isolated electrically driven guinea-pig and rat auricles, langendorff heart preparations of guinea-pigs and cat heart-lung preparations. low concentrations of toxin ii (2-100 nm) evoked a dose-dependent positive inotropic effect in the three different heart muscle preparations investigated. higher concentrations of toxin ii produced toxic symptoms like contracture and arr ... | 1976 | 12483 |
| the chemical structure of prostaglandin x (prostacyclin). | the chemical structure of prostaglandin x, the anti-aggregatory substance derived from prostaglandin endoperoxides, is 9-deoxy-6, 6alpha-epoxy-delta5-pgf1alpha. the stable compound formed when prostaglandin x undergoes a chemical transformation in biological systems in 6-keto-pgf1alpha. prostaglandin x is stabilized in aqueous preparations by raising the ph to 8.5 or higher. the trivial name prostacyclin is proposed for 9-deoxy-6, 9alpha-epoxy-delta5-pgf1alpha. | 1976 | 12538 |
| esterolysis of oxyprothepin decanoate in vitro proceedings. | | 1976 | 12641 |
| effects of the h1-antagonist promethazine and the h2-antagonist burimamide on chronotropic, inotropic and coronary vascular responses to histamine in isolated perfused guinea-pig hearts. | on guinea-pig atria part of the inotropic response to histamine is attributable to a concomitant increase of the frequency 7. since the chronotropic effect of histamine is mediated by a stimulation of h2-receptors a direct interaction of histamine with h1-receptors a direct interaction of histamine with h1-receptors mediating the inotropic response on heart may be overlooked. for this reason the ability of the h1-antagonist promethazine and the h2-antagonist burimamide to inhibit the positive ch ... | 1976 | 12652 |
| profile of pharmacological actions of nab 365 (clenbuterol), a novel broncholytic agent with selective activity on adrenergic beta2-receptors (author's transl). | effects of 4-amino-alpha-(tert.-butylamino)methyl-3,5-dichlorobenzyl alcohol hydrochloride (clenbuterol, nab 365) on the adrenergic beta-receptors were investigated and compared with those of isoproterenol and salbutamol. the beta2-mimetic activity of clenbuterol on the smooth muscle of bronchi, uterus and vessels after i.v. injection corresponds to that of salbutamol in all laboratory animals. when given subcutaneously or as an aerosol clenbuterol is even somewhat more effective than isoprotere ... | 1976 | 12768 |
| studies on digestion and absorption in the intestines of growing pigs. measurements of the flow of digesta and ph. | 1. thirty-five pigs were fitted with single re-entrant cannulas in either the duodenum, jejunum or ileum. a further twenty-four pigs were used in a conventional digestibility trial. 2. methods for collecting, sampling and returning digesta were developed. 3. a 'practical-type' diet and two purfied diets were used, fed twice daily. 4. flow and ph of digesta were measured hourly in the duodenum and jejunum, and every 6 h in the ileum. 5. in the duodenum and jejunum there were clear flow responses ... | 1976 | 12785 |
| effect of alcohols on the structure and function of d-amino-acid oxidase. | the absorption spectrum of d-amino-acid oxidase (d-amino-acid:oxygen oxidoreductase (deaminating), ec 1.4.3.3) was significantly perturbed by various alcohols; typical fine structures were observed in the visible absorption bands, accompanied by blue shifts of the peaks. both fluorescence intensity and fluorescence polarization were increased upon the addition of alcohols, indicating that the coenzyme is not liberated from the apoenzyme but the hydrophobicity of the environment of the enzyme-bou ... | 1977 | 12795 |
| mechanism of the inhibitory effect of glyoxylate plus oxaloacetate and oxalomalate on the nadp-specific isocitrate dehydrogenase. | the effects of glyoxylate plus oxaloacetate and of oxalomalate on the nadp-linked isocitrate dehydrogenase (threo-ds-isocitrate:nadp+ oxidoreductase (decarboxylating, ec 1.1.1.42) from pig heart from been studied with steady state methods as well as with stopped flow technique. when equimolar mixtures of glyoxylate and oxaloacetate were premixed for different lengths of time prior to addition to the assay mixture, the extent of inhibition increased with the premixing time. the results indicated ... | 1976 | 12805 |
| on the catalytic and binding sites of porcine enteropeptidase. | the active site of porcine enteropeptidase (ec 3.4.21.9) was investigated in order to characterize better both catalytic and binding sites. the participation of a serine and a histidine residue in the catalytic process was fully confirmed and the two residues were located on the light chain of the enzyme. the binding site was found to be composed of at least 2 subsites s1 and s2. the subsite s1 (similar to the trypsin-binding site) is responsible for the interactions with the small substrates of ... | 1976 | 12810 |
| proton transport by gastric membrane vesicles. | a highly purified membrane fraction was derived from hog gastric mucosa by a combination of differential and density gradient centrifugation and free flow electrophoresis. this final fraction was 35-fold enriched with respect to cation activated ouabain-insensitive atpase. antibody against this fraction was shown to be bound to the luminal surface of the gastric glands. the addition of atp to this fraction or the density gradient fraction resulted in h+ uptake into an osmotically sensitive space ... | 1977 | 12816 |
| effects of stereochemical structures of tetrahydrobiopterin on tyrosine hydroxylase. | 1. four stereochemical isomers of tetrahydrobiopterin, i.e., 6-l-erythro-, 6-d-erythro-, 6-l-threo-, or 6-d-threo-1,2-dihydroxypropyltetrahydropterin, have been synthesized and used as cofactors for tyrosine hydroxylase (ec 1.14.18.-) purified from the soluble fraction of bovine adrenal medulla. the l-erythro- (the putative natural cofactor) and d-threo isomers showed a striking similarity in their cofactor activities for tyrosine hydroxylase; the remaining two isomeric tetrahydrobiopterins, d-e ... | 1977 | 12820 |
| purification and characterization of cholesterol esterase from porcine pancreas. | a protein catalyzing the hydrolysis of cholesterol esters and p-nitrophenyl acetate has been purified 200-fold from porcine pancreas. the enzyme is homogenous as judged by polyacrylamide gel electrophoresis and exhibits a molecular weight of 80 000 as determined by sodium dodecyl sulfate electrophoresis and gel filtration. activity toward p-nitrophenylacetate exhibits a broad ph optimum and is influenced by a group with a pka of 5.5--6.0. the enzyme is completely inhibited by diisopropylfluoroph ... | 1976 | 12831 |
| analysis of the inotropic: chronotropic selectivity of dobutamine and dopamine in anaethetised dogs and guinea-pig isolated atria. | the previously reported in vivo inotropic selectivity of dobutamine and dopamine compared with isoprenaline has been demonstrated in anaesthetised bivagotamised open chest dogs. responses of heart rate, right ventricular tension, left ventricular dp/dtmax, and blood pressure were measured. compared with isoprenaline, dobutamine and dopamine were 1.8 and 2.4 times more active in increasing cardiac contractility than rate. the inotropic selectivity of dopamine but not that of dobutamine was abolis ... | 1977 | 12867 |
| esterolytic activities of rat intestinal mucosa. 1. characterization, cellular distribution and subcellular localization of a glycerol-ester hydrolase. | the preferential cellular distribution in the villus tip and the subcellular localization in the endoplasmic reticulum of an intestinal glycerol-ester hydrolase from rat mucosa are described. the enzyme is shown not to be from either pancreatic or bacterial origin; it catalyzes the hydrolysis of short- and medium chain triglycerides and of p-nitrophenylacetate. contrarily to the specificity found for the pig intestinal lipase (serrero, négrel and ailhaud, 1975), no activity is detectable against ... | 1976 | 12956 |