| separate cell types that express two different forms of somatostatin in anglerfish islets can be immunohistochemically differentiated. | the somatostatin-related peptides somatostatin-14 (ss-14) and somatostatin-28 (ass-28) are synthesized at the c-terminal end of two separate pre-pro-somatostatins in anglerfish pancreatic islets. the purpose of this study was to determine whether these peptides are expressed in the same or different cell types. antisera r141 and r293, which recognize the central region of ss-14 and the c-terminal region of ass-28 ([tyr7,gly10] ss-14), respectively, were used in an immunohistochemical examination ... | 1987 | 2878951 |
| effects of somatocrinin and a somatostatin antiserum on body and organ growth in the rat. | chronic treatment with rgrf for 14 days with or without a specific antibody against somatostatin-14 were performed on 24-day old male sprague-dawley rats. serum gh concentrations were significantly increased at the time of sacrifice while growth was not affected. rgrf, possibly through its effect on gh release, stimulated lung and kidney weights, increasing water content of the latter. muscle and testes remained insensitive to grf while the heart showed signs of reduced growth. liver growth resp ... | 1986 | 2879776 |
| changes in nonspecific immunity factor in some equidae, camelidae and capridae species. | plasma lysozyme activity changed during the 2 years of the investigation period. in some species (e.g. ponies and goats) there are only seasonal fluctuations with the highest levels during summer and autumn in ponies and during winter in goats. in two camelidae species, guanacos and llamas, only, downward trends were found, whereas in donkeys, both types of changes: seasonal variations with the maximal level during autumn and an upward long-term trend were found. | 1987 | 2882892 |
| direct evidence for two distinct prosomatostatin converting enzymes. detection using a rapid, sensitive, and specific assay for propeptide converting enzymes. | many bioactive peptides are initially synthesized via larger precursors from which they are released by proteolytic cleavage at basic amino acids. some precursors contain more than one final product peptide, multiple copies of a single peptide, or both. different product peptides can be produced from a common precursor in different tissues. it is not currently known whether this cell-type specific production of bioactive peptides is mediated by different, specific propeptide converting enzymes ( ... | 1987 | 2883185 |
| vector potential of culicine mosquitoes in faiyum governorate, egypt. | culicine mosquito populations were studied for 1 year in 2 neighboring villages in faiyum governorate, egypt. dominant species in larval collections included culex pipiens, cx. univitattus, cx. antennatus, aedes caspius and uranotaenia unguiculata; culiseta longiareolata was less common. culex pipiens accounted for 98% of 3,743 mosquitoes captured in human-biting collection (164 man-nights), 96% of 1,136 mosquitoes collected inside houses and animal sheds (396 inspections), and 98% of 15,000 mos ... | 1986 | 2906968 |
| [individual glycoside therapy using serum concentration determination in heart insufficiency of horses]. | 23 horses and one donkey with congestive heart failure are treated with a standardized methyldigoxin dose (0.0032 mg/kg of body weight). the therapy is controlled by the serum concentration of the cardiac glycoside. 4 horses have a higher and 13 horses a lower serum concentration as necessary for therapeutic approach. the influence of additional diseases and medications is demonstrated. finally a rule for the evaluation of the individual therapeutic glycoside-dose is given. | 1989 | 2923617 |
| [effects of dithiothreitol and thioredoxines on the in vitro activity of enzymes of the urea cycle in rats]. | the five urea cycle enzymes were studied in desactivated extracts of rat liver. after reduction by dithiothreitol (dtt) and in presence of mg2+ ions, thioredoxines isolated from rat liver were able to activate carbamyl phosphate synthetase-i (cps-i) and argininosuccinate synthetase (ass) respectively by 468% and by 370%. thioredoxines were purified from adult rat liver and an antiserum was raised to these proteins. after immunologic quantitation, their level in adult rat was 0.103 mg/g liver. | 1986 | 2948627 |
| blood ammonia levels and hepatic encephalopathy induced by ccl4 in rats. | an investigation of the mechanism of development of hepatic encephalopathy induced by ccl4 was performed in rats. ccl4 (1.0 ml/kg three times per week for over 10 weeks) caused hepatic encephalopathy in 80% of the treated rats. accompanying the hepatic encephalopathy were hematemesis, abdominal dropsy, and hyperammonemia, conditions observed in hepatic coma patients. the blood ammonia levels were tremendously increased in only those rats with hepatic encephalopathy. hepatic activities of carbamy ... | 1987 | 2962338 |
| parasites of the stomach in donkeys of the highveld of zimbabwe. | fourteen donkeys from a communal area of the zimbabwean highveld were examined for stomach parasites during july and november of 1986 and january and april of 1987. all 14 animals were infected. habronema muscae occurred in 12, draschia megastoma in 11, h. majus in 9, trichostrongylus axei in 9, gasterophilus intestinalis in 14 and g. nasalis in 1 donkey. the lowest level of infection was in january for the helminths and in april for gasterophilus larvae. the peak burdens of parasites were in ju ... | 1988 | 2975414 |
| [isolation of strains of a new ecological variant of staphylococcus aureus from asses]. | 47 coagulase-positive staphylococcal strains were isolated from the nasal smears of 175 healthy donkeys. in accordance with the schemes of akatov--devriese and mayer-witte--akatov, 10.6% of the cultures were classified with the coagulase-positive species s. hyicus and 89.3%, with the species s. aureus. out of s. aureus strains, 11.9% were found to have the characteristics of ecovar hominis, while 16.2% of the cultures could not be classified with definite ecovars. most of the strains (71.4%) wer ... | 1988 | 2975451 |
| serologic surveillance for vesicular stomatitis virus on ossabaw island, georgia. | seventeen species of mammals and seven species of birds from ossabaw island, georgia, were tested for vesicular stomatitis (vs) neutralizing antibodies. seropositive results were restricted to mammals with six of 17 species testing seropositive for vs (new jersey type) neutralizing antibodies. seropositive species included: raccoons (procyon lotor), white-tailed deer (odocoileus virginianus), feral swine (sus scrofa), cattle (bos taurus), horses (equus caballus), and donkeys (equus asinus). all ... | 1985 | 2987546 |
| replication of alphaviruses in cultures of donkey monocytes. | representative strains of venezuelan equine encephalitis virus (veev) and eastern equine encephalitis virus (eeev) were compared for their ability to grow in cultures of unstimulated leucocytes and monocytes derived from donkey peripheral blood. replication of epizootic and vaccine strains of veev, but not of enzootic strains was observed in this system. only a minority of monocytes supported virus replication as detected by immunofluorescence, electron microscopy and infectious center assays. e ... | 1986 | 3004384 |
| xenobiotic-metabolizing enzyme activity in human non-small-cell derived lung cancer cell lines. | human lung cancer cell lines in culture were investigated for the expression of monooxygenase and other xenobiotic-metabolizing enzyme activities. two bronchiolo-alveolar carcinoma derived cell lines (nci-h322 and nci-h358) and two small-cell carcinoma derived cell lines (nci-h128 and nci-h69) were used. previous work has shown that nci-h322 has ultrastructural features of clara cells while nci-h358 shows characteristics of alveolar type ii cells [schuller et al., proc. am. ass. cancer res. 26, ... | 1986 | 3004505 |
| studies of ebv-lymphoid cell interactions in two patients with the x-linked lymphoproliferative syndrome: normal ebv-specific hla-restricted cytotoxicity. | two x-linked lymphoproliferative syndrome (xlp) patients with the hypogammaglobulinemia phenotype were investigated at a time remote from their primary infection with the epstein-barr virus (ebv). the lymphoblastoid cell lines derived from these patients expressed the phenotypic markers characteristic of normal mature b lymphocytes and produced normal levels of immunoglobulins (ig). these observations imply that at least some of their b cells are phenotypically normal. the natural killer (nk) ac ... | 1986 | 3009061 |
| hormonal regulation of four urea cycle enzymes in postnatal rat liver in organ culture. | the administration of hydrocortisone to 3- to 15-day-old rats increased the levels of hepatic argininosuccinate synthetase (ass) and arginase. in 13-day-old rat liver explants maintained in organ culture, ornithine carbamoyltransferase (otc), carbamoylphosphate synthetase (cps) and arginase were stimulated by betamethasone. actinomycin d prevented the responses of the latter two enzymes. dibutyryl cyclic amp raised otc, cps, ass and arginase in vitro. the responses of the latter three enzymes we ... | 1985 | 3013609 |
| effects of pancreatic hormones and glucocorticosteroids on argininosuccinate synthetase and argininosuccinase activities of rat liver during the perinatal period: in vivo and in vitro studies. | the activity changes of two urea cycle enzymes, argininosuccinate synthetase (ass) and argininosuccinase (asl), were followed after corticosteroid and pancreatic hormone treatments in utero and in primary cultured fetal hepatocytes. the asl activity which was induced by glucagon or by (bu)2camp administration was enhanced by a treatment with streptozotocin for 2 days, although ass was not changed under these conditions. the activity of both enzymes was enhanced by cortisol administration in uter ... | 1986 | 3015569 |
| poxvirus infection in a donkey. | | 1986 | 3022455 |
| comparison of horseradish peroxidase and alkaline phosphatase-labelled antibodies in enzyme immunoassays. | the periodate method was found to be most effective for preparing horseradish peroxidase-sheep anti-human and horseradish peroxidase-donkey anti-mouse immunoglobulin (igg) conjugates. the conjugates were improved by carrying out the oxidation of the enzyme at low ph. anti-human and anti-mouse igg-peroxidase conjugates (0.5 mg/ml igg and 0.7 mg/ml igg, respectively) were used at 1:15,000 and 1:8000 dilutions, respectively, in a sandwich elisa to detect human and mouse igg in buffer or in a growth ... | 1987 | 3035992 |
| [breast feeding as "alternative" nutrition? an orientation aid in the field spanning ideology and current status]. | recommending breast feeding supports a trend "back to nature". however, there are believers in the unbiased goodness of nature, who claim effects of mothers milk, e.g. protection from the evils of civilization, that are not confirmed by current scientific knowledge. as nature is not uniquely good (it feeds the donkey and the lion), and as biology is not perfect, breast feeding does not guarantee an uneventful infancy and childhood. there is even a pathology of breast feeding that the pediatricia ... | 1988 | 3043205 |
| [short-lasting infusions of fibraccel following aortocoronary bypass surgery. the effect on postoperative blood loss and hemostasis depending on the time of extracorporeal circulation]. | bleeding is a major problem in the post-operative period (p.o.) after aortocoronary bypass surgery. harke et al. have investigated the beneficial effect of homologous phospholipid complex (fibraccel) after mitral and/or aortic valve replacement. no actual data were available evaluating therapy with fibraccel after aortocoronary bypass grafting. we were especially interested in the influence of fibraccel on routine blood coagulation parameters: partial thromboplastin time (ptt), thrombin time (tt ... | 1988 | 3056085 |
| determination of proinsulin intermediates by means of an immunoradiometric method using polyclonal antibodies. | in our endeavour to develop a method for proinsulin determination, an immunoradiometric method was developed. thereby guinea-pig antibodies to bovine insulin which were purified with an immunoadsorbent, were, in surplus, coupled to polyethylene tubes. these tubes were used to extract proinsulin and insulin from the sample and standard that or was to be determined. the proinsulin adsorbed onto the wall of the tube was distinguished from insulin by incubating, in a second step, a rabbit antibody t ... | 1988 | 3056811 |
| reproductive characteristics of the jenny donkey--equus asinus: a review. | | 1988 | 3057709 |
| isolation of a physiologically active and a physiologically inactive mitochondrial nadh-ubiquinone reductase (complex i) from donkey hearts. | the method described for the isolation of mitochondrial complex i (nadh-ubiquinone reductase) from bovine hearts could not be applied to donkey hearts as unacceptably large losses in enzyme activity occurred. this method was modified for the isolation of complex i using donkey hearts and two complexes were obtained: complex ia which was physiologically inactive and complex ib which was physiologically active as it catalyzed the reaction from nadh to ubiquinone. both complexes had relatively low ... | 1986 | 3085542 |
| nucleotide sequence of the 26 s mrna of the virulent trinidad donkey strain of venezuelan equine encephalitis virus and deduced sequence of the encoded structural proteins. | a cdna clone containing all of the 26 s mrna coding region of the rna genome of venezuelan equine encephalitis (vee) virus, virulent strain trinidad donkey (trd), has been constructed and sequenced. the nucleotide and deduced amino acid sequences of the 26 s rna of vee virus conform to the general organization of the alphavirus subgenomic mrna. excluding the poly(a) tail, the vee 26 s rna is 3913 nucleotides long with a protein coding region of 3762 nucleotides. codon usage in the translated reg ... | 1986 | 3088830 |
| [effects of dtt and thioredoxins on rat argininosuccinate synthetase activity in vitro]. | argininosuccinate synthetase (ass, ec 6.3.4.5), the third enzyme of urea-cycle, was studied in desactivated extracts of rat liver. the enzyme is activated, in vitro, by mg2+ ions (5 mm) and dithiothreitol (dtt: 10 mm). after reduction by dtt, thioredoxins isolated from rat liver were able to activate ass by 370%. | 1986 | 3091197 |
| ultrastructure of sarcocystis spp. from donkeys (equus asinus) in egypt. | the fine structure of sarcocystis spp. from donkeys (equus asinus) in egypt is described. sarcocysts were found in the oesophagus, diaphragm and heart of 18 of 20 donkeys. only one type of mature muscle cyst was found. sarcocysts were 120-410.6 x 48.4-50.2 microns. the primary cyst wall had numerous 3.3-3.7 microns villi. each villus contained 20-60 fibrillar elements which extended from the ends and sides of the villi throughout the ground substance, where they became tightly packed. the bundle ... | 1987 | 3105164 |
| biological and immunological properties of zebra pituitary gonadotropins: comparison with horse and donkey gonadotropins. | previous studies from this laboratory have described the properties of purified luteinizing hormone (lh) and follicle-stimulating hormone (fsh) from horse and donkey anterior pituitary glands. the present study afforded the opportunity to further characterize these previously purified hormone preparations and to compare them with enriched gonadotropin fractions from zebra pituitary glands. although a single lh and fsh fraction was usually obtained for each pool of pituitaries, two separate zebra ... | 1987 | 3113497 |
| chemical heterogeneity of amyloid in the carpal tunnel syndrome. | 140 biopsies from 108 patients afflicted with the carpal tunnel syndrome were studied, 27 of whom showed deposition of amyloid, in 6 of them to such an extent that the amyloid was considered significant in the pathogenesis of the carpal tunnel syndrome. morphologically, vessels and ligaments were affected and especially the peritendinous structures. as it was always part of generalized amyloidosis, the amyloid in the carpal tunnel consisted immunohistologically of amyloid a in three cases (inclu ... | 1987 | 3120402 |
| neisseria mule, horse, or donkey. | | 1988 | 3130392 |
| [acetylsalicylic acid pseudoallergy: an anomaly of thrombocyte function?]. | after a short historical review, the clinical symptomatology of the pseudoallergic reactions (par) after intake of acetylsalicylic acid (ass) is examined. an intolerance to ass mostly becomes manifest as bronchial asthma--sometimes up to a status asthmaticus: it is frequently combined with vasomotoric rhinopathia and nasal polyps (so-called "aspirin triad") or as urticaria and angio-edema, seldom as a shock reaction. these symptoms can - in contrast to an allergy--appear at the first intake of t ... | 1988 | 3148593 |
| macroscopic organization and sensitive innervation of the tendinous intersection and the lacertus fibrosus of the biceps brachii muscle in the ass and horse. | the most developed and organized lamina running trough the biceps brachii muscle belly forms a well-marked tendinous intersection connecting the proximal tendon with the distal one. moreover, the lacertus fibrosus arises from this lamina close to the distal tendon and blends with the fascia of the forearm and joins the extensor carpi radialis muscle. the nerve supply for the biceps tendinous intersection arises from the intramuscular rami of the musculo-cutaneous nerve, whereas the lacertus fibr ... | 1986 | 3153560 |
| arteria linguofacialis of the donkey (equus asinus). | | 1985 | 3158250 |
| comparison of fiber types in skeletal muscles from ten animal species based on sensitivity of the myofibrillar actomyosin atpase to acid or copper. | comparisons were made of the histochemical characteristics of skeletal muscle from 10 animal species. the basic comparison was made from the staining patterns for the myofibrillar actomyosin atpase produced by preincubation of fresh frozen cross-sections of muscle at alkaline ph (10.30) or acid ph (4.60) with those produced by preincubation in media containing cu2+ at alkaline ph (10.30), near neutral ph (7.40), or acid ph (4.60). muscle sections were also stained for reduced nicotinamide adenin ... | 1985 | 3158628 |
| lungworms (dictyocaulus arnfieldi): prevalence in live equids in kentucky. | prevalence of natural infections of the lungworm, dictyocaulus arnfieldi, was investigated in kentucky in 5,437 live equids (horses, ponies, and asses) on 91 farms by examination of fecal samples over a 15-month-period from january 28, 1983 to april 19, 1984. for the 91 farms investigated, asses only were examined on 1 farm; for the other 90 farms, examinations were made of horses on all of them, of ponies on 4 farms, and of asses on 9 farms. larvae of d arnfieldi were found in fecal samples of ... | 1985 | 3160271 |
| veins of the head and neck of the donkey (equus asinus). | | 1985 | 3161417 |
| [separation of rat serum antisuppressor antibodies eliminating t-suppressors and stimulating their generation in mice in vivo]. | immunization of rats with enriched murine specific t-suppressors (sts) permitted obtaining the antisuppressor serum (ass) which selectively inactivated in vitro the capacity of the sts to inhibit the proliferation of t-lymphocytes in a mixed lymphocyte culture in response to allo-antigens. two opposite effects of the ass in vivo were demonstrated: elimination of t-suppressors (on ass administration 4 days after immunization) and stimulation of their formation (on ass administration before immuni ... | 1985 | 3161559 |
| centric fission in the karyotype of a mother-daughter pair of donkeys (equus asinus). | a mother-daughter pair of donkeys was found to have identical karyotypes with a diploid number of 63. the difference from the species karyotype could be explained by a centric fission event in the third largest autosomal pair. | 1988 | 3163968 |
| recombinant vaccinia virus/venezuelan equine encephalitis (vee) virus protects mice from peripheral vee virus challenge. | mice immunized with recombinant vaccinia virus (vacc) expressing venezuelan equine encephalitis (vee) virus capsid protein and glycoproteins e1 and e2 or with attenuated vee tc-83 virus vaccine developed vee-specific neutralizing antibody and survived intraperitoneal challenge with virulent vee virus strains including trinidad donkey (subtype 1ab), p676 (subtype 1c), 3880 (subtype 1d), and everglades (subtype 2). however, unlike immunization with tc-83 virus, immunization with the recombinant va ... | 1988 | 3184276 |
| effects of intravenous corticosteroid on the pathogenicity of babesia equi infection of donkeys (equus asinus). | | 1988 | 3188726 |
| the pattern of venous drainage of the equine ileocaecal junction. | the veins draining the ileocaecal junctions of horses (n = 19), donkeys (n = 3) and a plains zebra, (equus burchelli antiquorum) were injected with latex via the ileocolic vein, and dissected. in all specimens the ileocaecal papilla was drained by 2 major papillary veins: one cranial and one caudal to the papilla. a smaller dorsal vein drained either into the cranial or into the caudal vein. the submucosal veins seemed to increase in number in the ileocaeacal junction to form a venous plexus. th ... | 1988 | 3199408 |
| clinical parameters of donkeys before and after trypanosoma vivax infection. | | 1988 | 3201022 |
| [the body as hostage of the self. phenomenological aspects of anorexia nervosa]. | there are several basic structures upon which anorexia nervosa could develop: hostility between mother and daughter, feministic protest, abandonnism, the ascetic structure, the reluctance against the being-thrown-on-the-world, asw. the phenomenological analysis reveals, through these different structures, a common distress. the patient feels the others, and also, the existence, as a violence. yet, she feels help ass in her fight against this violence. so she takes her own body as hostage in orde ... | 1988 | 3207043 |
| ribosomal rna expression in a mammalian hybrid, the hinny. | the expression of nucleolus organizer region (nor) activity in diploid cells was investigated in a model mammalian hybrid system, the hinny (female ass x male horse), by sequential ag-nor and chromomycin a3/distamycin a/dapi (cdd) staining ion lectin-stimulated peripheral blood lymphocytes. in the majority of cases we found non-expression of the horse-derived nor chromosomes in the hinny. however, in one case there was strong nor expression on horse-derived chromosome no. 1. | 1988 | 3219914 |
| keratinophilic fungi on the hair of cows, donkeys, rabbits, cats, and dogs from the west bank of jordan. | the mycoflora of hair of 111 cows, donkeys, rabbits, cats, and dogs were analysed and the frequency of occurrence and the relative importance value of the different fungal species isolated were calculated. total numbers of species 56, 45, 48, 23, and 11 were recovered from cows, donkeys, rabbits, cats, and dogs respectively. the majority of the keratinophilic fungi isolated were either well-known mycotic agents or have been recovered from various animal and human lesions. these comprised 87.8%, ... | 1988 | 3221913 |
| [effect of various combinations of dl-alpha-tocopherol and acetylsalicylic acid on adjuvant arthritis in the rat]. | the influence of per os application of different combinations of dl-alpha-tocopherol (toc) and acetylsalicylic acid (ass) on adjuvant-induced arthritis was tested in male wistar rats (body weight 227 +/- 18 g; 8 groups, n = 8). one group (control) was without adjuvans arthritis and received no treatment, another group was also not treated in spite of adjuvans arthritis. further, six groups of adjuvans arthritis were treated with the following combinations: ass/toc (mg/kg bw/d each) 250/-, 250/25 ... | 1988 | 3239266 |
| the primary structure of donkey (equus asinus) lysozyme contains the ca(ii) binding site of alpha-lactalbumin. | the complete primary structure of donkey lysozyme has been established by pulsed liquid-phase sequencing of tryptic and chymotryptic peptides isolated by rp-hplc. the positions of the cys residues were identified by labeling the cys residues with dabia-reagent. donkey lysozyme is a c-type lysozyme which is 129 amino acids long. it exhibits 50% homology to the human protein. we observe the full ca(ii) binding site suggested for the homologous alpha-lactalbumines. although horse lysozyme has been ... | 1988 | 3242541 |
| [botfly larva skin infestation in a donkey]. | in january 1987 7 larvae of hypoderma diana were found in a donkey. the infection probably took place on a horse pasture in fürstenwalde, frankfurt (o.) region, grazed also by roes, the main hosts of h. diana. | 1988 | 3245636 |
| factors influencing anti-antibody enhancement of tumor targeting with antibodies in hamsters with human colonic tumor xenografts. | the injection of an antiantibody (second antibody, sa) can enhance the clearance rate of a radiolabeled antitumor antibody (primary antibody, pa) from the blood. we have studied how the dose of the sa and the timing of the sa administration influence the rate of pa clearance and thereby improve tumor/nontumor ratios. adult hamsters bearing the carcinoembryonic antigen-producing, gw-39 human colonic tumor xenograft were given injections of 131i-labeled, goat anti-carcinoembryonic antigen antibody ... | 1988 | 3258181 |
| interspecific pregnancy: barriers and prospects. | investigations on the mechanisms that allow survival of the fetal allograft have been extended by pregnancies in which the fetus and pregnant female are from different species. such interspecific pregnancies are useful models for the study of maternal/fetal interactions and also may assist in the preservation of endangered species. results of experiments with three different interspecific model systems are discussed: a murine model using mus musculus and m. caroli; an equine model involving prim ... | 1988 | 3284594 |
| clinical application of enzyme immunoassay in the analysis of citrullinemia. | we have developed a sensitive enzyme immunoassay (eia) for the quantification of argininosuccinate synthetase (ass) in the range of 0.05-1 ng/tube using a fab'-peroxidase conjugate prepared with maleimide compound devised by ishikawa et al. amounts of hepatic ass in control subjects were determined by this method with the purified enzyme as a standard and their specific activities were calculated to be 1.3-1.4 mumol product/min (u)/mg of ass, which was quite similar to that of the purified enzym ... | 1987 | 3297428 |
| [prevention of migraine with flunarizine and acetylsalicylic acid. a double-blind study]. | 30 children between 7 and 17 years suffering from at least 2 attacks/month of common or classical migraine since more than 1 year were studied. after clinical exclusion of symptomatic headache 4 weeks were documented by means of a migraine diary. prophylaxis with calcium entry blocker flunarizine (sibelium) or thromboxane a inhibitor acetylsalicylic acid (ass) was carried out in a double blind design for 3 months. medication was given as one dosage in the evening: 2-5 mg/kg kg ass or 5-10 mg flu ... | 1987 | 3313023 |
| induction of the five urea-cycle enzymes by glucagon in cultured foetal rat hepatocytes. | foetal hepatocytes obtained from rats at different stages were cultured in order to investigate the inducibility of the five urea-cycle enzymes by glucagon and dibutyryl cyclic amp (bt2camp). when 18.5-day-old hepatocytes were cultured for 3 days with 10(-7) m glucagon, the activities of carbamoyl phosphate synthetase (cps), argininosuccinase (asl) and arginase were increased by 1.4-, 1.8- and 1.9-fold, respectively, as compared to controls. these effects were mimicked by 10(-4) m bt2camp, but t ... | 1987 | 3328726 |
| selection for accelerated penetration in cell culture coselects for attenuated mutants of venezuelan equine encephalitis virus. | previous studies with sindbis virus (sb) suggested that a single point mutation in glycoprotein e2 (serine 114 to arginine 114) conferred three phenotypic alterations: attenuation in neonatal mice, accelerated penetration of cultured cells, and efficient neutralization by two e2-specific monoclonal antibodies (davis, fuller, dougherty, olmsted, and johnston (1986) proc. natl. acad. sci. usa 83, 6771-6775). moreover, selection for rapidly penetrating mutants of sb coselected for attenuation in vi ... | 1988 | 3341117 |
| an elisa for the measurement of vp16 (etoposide) in unextracted plasma. | an elisa for the anti-cancer drug vp16 in unextracted plasma has been developed using vp16-thyroglobulin-coated microtitre plates, a sheep anti-vp16 serum, and a donkey anti-sheep hrpo-labelled second antibody. the sensitivity of the assay was 0.5 ng/ml, providing a detection limit of 0.1 microgram vp16/ml plasma. plasma interference effects were negligible and therefore the standard curve could be set up in assay buffer. a good correlation was obtained between the elisa and established hplc and ... | 1988 | 3346552 |
| isolation of pure babesia equi and babesia caballi organisms in splenectomized horses from endemic areas in south africa. | both babesia equi and babesia caballi are endemic in large parts of south africa. attempts were made to obtain pure local isolates of both b. equi and b. caballi for the purpose of developing serological tests to study the epidemiology of equine babesiosis in this country. the indirect fluorescent antibody test was used to screen horses for b. equi and b. caballi in an endemic area. seven horses and 3 donkeys between 3 and 36 months of age that tested negative were subsequently splenectomized. t ... | 1988 | 3353098 |
| microanalysis of the amino-acid sequence of monomeric beta-lactoglobulin i from donkey (equus asinus) milk. the primary structure and its homology with a superfamily of hydrophobic molecule transporters. | the complete primary structure of donkey beta-lactoglobulin i was determined by pulsed-liquid phase microsequencing of tryptic peptides. the protein has been isolated in monomeric form and it corresponds to monomeric beta-lactoglobulin of type i. with the inclusion of donkey beta-lactoglobulin i there are 13% common residues amongst the members of the beta-lactoglobulin family. donkey beta-lactoglobulin i is homologous to the retinol-binding protein, bilin-binding protein and five other proteins ... | 1988 | 3370127 |
| a fertile mule and hinny in china. | anecdotal reports of fertility in female mules (jack donkey x mare) and hinnies (stallion x jenny donkey) have appeared in the literature over the years, but scientists have generally regarded them with scepticism. the fact that some of these hybrids can come into estrous and ovulate makes fertility conceivable, given that opportunity for mating arises. in china, where mules are bred extensively for work on the farms, a fertile female mule and a fertile female hinny have now been verified by chr ... | 1988 | 3378453 |
| recovery of leishmania (viannia) braziliensis from inoculated hamsters. | leishmania (viannia) braziliensis has never been isolated from wild animals although it is apparently capable of inducing infections in man, dogs, and donkeys. an analysis of the standard hamster culture system for analyzing infectivity of leishmania sp. was undertaken. results indicate that for l. (v.) braziliensis, routine cultivation of aspirates taken from the inoculation sites of 1-mo-infected hamsters should be undertaken. moreover, in at least 1 of the 3 strains examined, isolation of the ... | 1988 | 3379536 |
| interspecies variation in milk composition among horses, zebras and asses (perissodactyla: equidae). | milk samples of four species of wild equids (onager, equus hemionus onager; mountain zebra, e. zebra hartmannae; plains zebra, e. burchelli; przewalski horse, e. caballus przewalskii) and two domesticated equids (ass, e. asinus; pony, e. caballus) were analysed. at mid to late lactation the milks of all species were very similar, containing on average 10-12% total solids, 1-2% fat, 1.6-1.8% true protein, 6-7% 'lactose', 0.3-0.5% ash, 0.08-0.12% calcium, 0.04-0.07% phosphorus and a calculated ene ... | 1988 | 3385068 |
| [cachexia syndrome in a donkey mare caused by toxic infectious enteritis. 1. primary symptomatology and pathology]. | | 1988 | 3390131 |
| [cachexia syndrome in a donkey mare caused by toxic infectious enteritis. 2. anemia as a secondary symptom complex]. | | 1988 | 3390132 |
| the arrangement of the muscle layers at the equine ileocaecal junction. | the muscle layers of the ileocaecal junctions of horses (n = 18), plains zebras (equus burchelli antiquorum) (n = 3), mountain zebras (equus zebra zebra) (n = 2), and a donkey were examined macroscopically and microscopically. the muscular tunic of the ileocaecal papilla was made up of 3 layers: an innermost circular layer which was a continuation of the circular muscle of the ileum, a central longitudinal layer which was a continuation of one part of the longitudinal layer of the ileum and an o ... | 1988 | 3392703 |
| [cachexia syndrome in a donkey mare due to a toxic infectious enteritis. 3. hyperlipemia as a secondary complication]. | | 1988 | 3401205 |
| urinary thallium: critical problems, reference values and preliminary results of an investigation in workers with suspected industrial exposure. | using a sensitive eta ass method, urinary thallium (tl-u) has been measured in a group of non-exposed individuals and in two groups of workers with suspected industrial exposure. in non-exposed subjects (72 healthy males aged 41 +/- 11 years) the tl-u mean value was 0.22 mu/l (range 0.05-0.61). significantly higher values were found in two groups of workers (30 and 21 subjects) employed in two cement factories and two cast iron foundries (mean tl-u value: 0.38 and 0.33 mu/l, respectively; range ... | 1988 | 3406716 |
| [mortality and morbidity of thromboembolism in drug prevention--5-year analysis]. | in a period of five years a general medical prevention of postoperative venous thromboembolic disease was tested in major gynaecological operations, in patients undergoing radium insertions for gynaecological cancer and in patients with caesarean section. preoperatively was the beginning of the daily therapy with dihydroergotamine (dhe) and every four days the therapy with acetylsalicylic acid (ass). the results of 2,346 therapy-cycles with medical prevention of thromboembolic disease in 2,280 p ... | 1988 | 3407358 |
| hepatic atp content and hyperammonemia induced by ccl4 in rats. | an investigation of the mechanism of development of hyperammonemia observed in ccl4-induced hepatic encephalopathy was performed in rats. ccl4 (1.0 ml/kg 3 times per week for over 10 weeks) caused a severe hyperammonemia and depletion of hepatic atp contents in only those rats with hepatic encephalopathy. however, ccl4 (1.0 ml/kg 3 times per week for 7 weeks) did not cause hepatic encephalopathy and did not change in blood ammonia levels. administration of 2,4-dinitrophenol (2,4-dnp) in these cc ... | 1988 | 3413798 |
| use of high-performance liquid chromatography for the purification of antibodies and antibody conjugates and the study of antibody-antigen interactions. | the purification and characterization of polyclonal and monoclonal antibodies and antibody-enzyme conjugates by high-performance liquid chromatography (hplc) using a protein pak 300 sw column and a protein pak deae anion-exchange column is described. the following polyclonal antibodies were examined: (i) donkey anti-mouse immunoglobulin g (igg), (ii) mouse igg, (iii) sheep anti-human igg and (iv) goat anti-human factor viii. hplc was used to analyse the purity of horseradish peroxidase conjugate ... | 1988 | 3417819 |
| molecular basis of enzyme abnormalities in urea cycle disorders. with special reference to citrullinemia and argininosuccinic aciduria. | this paper deals with enzymological, immunochemical and molecular genetic analyses of citrullinemia and argininosuccinic aciduria. citrullinemia has been classified by saheki et al. [j. inher. metab. dis. 8: 155-156, 1985] into three types from the properties of the deficient argininosuccinate synthetase (ass) of the patients. analysis of hepatic mrna coding for ass revealed certain characteristics in type ii and iii citrullinemic patients whose hepatic ass protein was low. a newly developed enz ... | 1987 | 3440446 |
| the transstadial transmission of babesia caballi by rhipicephalus evertsi evertsi. | rhipicephalus evertsi evertsi larvae were fed on the ears of rabbits. seven days after larval infestation, unfed, newly moulted nymphae were manually removed to infest a splenectomized donkey showing a patent babesia caballi infection. engorged nymphae were collected from the donkey and the ensuing adult ticks were placed on a susceptible horse. the horse contracted a b. caballi infection showing a prepatent period of 19 days after tick infestation. a very low parasitaemia, (highest score 2), wh ... | 1987 | 3444624 |
| phase-derivative imaging. i: methods and stabilization analysis. | the potential of using the phase derivative (pd) for echographic imaging was investigated. the pd data were calculated by four methods: zero crossing (zcs) with squelch addition, analytic signal either with squelch addition (ass) or with employment of a wiener kernel (asw), and unwrapped phase (uwp). the large peaks which occur in an unprocessed pd signal were "stabilized" by some kind of smoothing algorithm. the effects of the amplitude of the squelch signal and of the degree of smoothing were ... | 1987 | 3445400 |
| [frequency of the isolation of staphylococci from domestic animals and strain identification]. | staphylococci occur in donkeys more frequently than in other animals, and only from donkeys coagulase-negative staphylococci, characteristic of humans (s. hominis, s. capitis, s. cohnii), were isolated. least frequently staphylococcal carrier state was registered in cats; in these animals only coagulase-negative strains were found to occur. from 30 donkeys coagulase-positive staphylococci belonging to 47 s. aureus strains were isolated. these strains differed from known ecological variants in th ... | 1987 | 3445728 |
| messenger rna coding for argininosuccinate synthetase in citrullinemia. | messenger rna coding for argininosuccinate synthetase (ass), extracted from the livers of some patients with citrullinemia, was analyzed using a cell-free translation system and dot and northern blot hybridization with cdna probe for ass. in patients with quantitative-type citrullinemia, called type ii here, previous studies have demonstrated that the hepatic content of the enzyme was about 10% of the control value, whereas the translatable mrna level for the enzyme was similar to that of contro ... | 1986 | 3459354 |
| extraspecific donkey-in-horse pregnancy as a model of early fetal death. | transfer of donkey embryos to horse mares provides a useful model of early fetal death. endometrial cups do not develop in this one type of extraspecific pregnancy and 80% of donkey fetuses are aborted between days 80 and 100 of gestation in conjunction with abnormal implantation and an intense accumulation of leucocytes in the endometrium of the surrogate mare. treatment of mares carrying donkey conceptuses with progestagen (allyl trenbolone) or purified horse chorionic gonadotrophin does not p ... | 1987 | 3479576 |
| clinical and endocrine aspects of the oestrous cycle in donkeys (equus asinus). | oestrous behaviour and occurrence of ovulation was studied in 13 jenny asses (3-18 years) during a 15-month period. teasing was performed daily and follicular growth, ovulation and changes of the genitalia were checked every other day by rectal palpation during dioestrus or the anovulatory season or daily during oestrus and when a follicle greater than or equal to 3 cm in diameter was present. plasma oestrogens and progesterone levels were measured by radioimmunoassay (ria). of the 13 jennies, 6 ... | 1987 | 3479584 |
| anti-antibody enhancement of iodine-131 anti-cea radioimmunodetection in experimental and clinical studies. | imaging of tumors with radiolabeled antibodies, especially when located in the blood-rich visceral organs, may be improved through administration of a second antibody directed against the primary tumor-associated antibody. in hamsters bearing a human colonic carcinoma xenograft producing carcinoembryonic antigen (cea), we injected donkey anti-goat igg 24 hr after administration of 131i-labeled goat anti-cea igg and achieved enhanced tumor imaging 24-48 hr later, with a significant relative decre ... | 1987 | 3498807 |
| evolution of nuclear gene families in primates. copy-number variation in the argininosuccinate synthetase (ass) pseudogene family and the anonymous dna sequence, d1s1. | changes in the copy number of nuclear genes provide the raw material for the creation of new gene functions. to better understand the mechanisms for such events, and their physiologic and evolutionary consequences, it is valuable to study a well characterized and closely related group of species such as primates. fortuitously, most of the powerful molecular techniques and dna probes developed for research in humans are equally applicable to non-human primates. we review what is known of copy num ... | 1987 | 3505891 |
| a technique for elective splenectomy of equidae using a transthoracic approach. | splenectomy was performed electively on 80 ponies, horses, and donkeys for preparation in collecting babesia antigens. access to the abdomen was by resection of the sixteenth left rib and incision of the diaphragm. the technique afforded good exposure of the splenic vessels and attachments. closure was facilitated by preservation of the periosteum of the resected rib. difficulty was encountered both in surgical technique and postoperative complications in obese animals. the transthoracic approac ... | 1987 | 3507171 |
| staphylococcus hyicus in donkeys. | | 1986 | 3513432 |
| apgar scores and asphyxia. results of a study and proposal for a clinical grading system. | the apgar score (as) was devised in 1953 as a simple system for classifying neonatal condition at 1 minute and was later modified to include status at 5 minutes. however, studies have documented pitfalls, e.g. a poor correlation between as and acid-base status. we studied 35 infants born at term. ass were assigned by two nurses not involved in the delivery, and antepartum and postpartum events were noted. we confirmed the following: (i) labour ward staff tend to overestimate ass; (ii) there is a ... | 1986 | 3518083 |
| visuomotor properties of neurons of the anterior suprasylvian gyrus in the awake cat. | single cell activity was recorded from the anterior suprasylvian (ass) gyrus of cats trained to orient their gaze toward visual or auditory stimuli. sixty-five fixation cells were activated or suppressed as long as the animals were attentive to a particular region of space in the tangential or in the radial direction. most of these fixation cells were neither light nor sound sensitive. fifty-five cells were activated in relation to saccades. fourteen neurons were active before and 41 after the o ... | 1986 | 3519268 |
| immunoassays for low concentrations of albumin in urine. | we have developed and validated simple, rapid immunoassays to measure concentrations of albumin in urine ranging from 5 to 200 mg/l. we use antiserum to human albumin, raised in sheep, and we separate the antibody-bound and free fractions of albumin by using a second antiserum, produced in donkeys, against the fc fragment of sheep igg. these two antisera can be mixed and added to assay tubes as a single reagent without inhibiting antigen binding. samples or standards are incubated for at least 3 ... | 1986 | 3521940 |
| development of a noncompetitive, solid phase, bridged biotin-avidin enzyme immunoassay for measurement of human leukocyte microsomal hmg-coa reductase protein concentration. | methods were developed for determination of human mononuclear leukocyte hmg-coa reductase protein concentration by a noncompetitive, solid phase, bridged biotin-avidin enzyme immunoassay procedure. leukocyte microsomal hmg-coa reductase, first immobilized onto a nitrocellulose filter, is sequentially reacted with 1) monospecific, polyclonal rabbit anti-rat liver hmg-coa reductase antiserum, which crossreacts with the human liver and leukocyte enzymes; 2) biotinylated donkey anti-rabbit immunoglo ... | 1987 | 3553404 |
| incidence and monthly prevalence of gasterophilus spp. larvae (diptera: gasterophilidae) in the stomach of donkeys (equus asinus) in egypt. | the stomachs of 118 donkeys were examined at postmortem during the period from march 1982 to february 1983 for gasterophilus spp. larvae. g. intestinalis larvae clustered in groups near the boundary of the glandular and non-glandular epithelium of the stomach and infested 98.3% of the donkeys with highest numbers in july and lowest numbers in october. g. nasalis larvae were mainly attached near the pylorus and first part of the duodenum and infested 87.3% of donkeys with highest incidence in dec ... | 1987 | 3564358 |
| long-term circulatory and respiratory support using two artificial ventricles and a fluorocarbon oxygenator. | using a new heart-lung machine developed at our institute, experiments were conducted on dogs, donkeys, and monkeys, providing complete and assisted artificial circulation, assisted oxygenation, and perfusion preservation of the heart. the new apparatus consists of two sequentially mounted artificial ventricles working in an antiphase mode; blood oxygenation is accomplished by a reusable oxygenator with a fluorocarbon preoxygenator. experimental results indicate that clinical use of the apparatu ... | 1987 | 3566581 |
| five cases of psoroptic mange in the domestic donkey (equus asinus asinus) and treatment with ivermectin. | | 1987 | 3569197 |
| structure of an abnormal messenger rna for argininosuccinate synthetase in citrullinemia. | the structural abnormality of mrna for argininosuccinate synthetase (ass) and the structure of immune cross-reactive material for ass (ass-crm) in the liver of a patient with type iii citrullinemia were analyzed using dot and northern blot hybridization, s1 nuclease analysis, and a sensitive enzyme-linked immunosorbent assay. the patient's liver contained no detectable ass activity, the ass-crm content was about 0.5% of that in the control and the ass mrna content was almost normal. the ass mrna ... | 1987 | 3570300 |
| [natural infection of equus asinus by leishmania braziliensis braziliensis-bahia, brazil]. | in corte de pedra, valença, state of bahia, a donkey, equus asinus, was found naturally infected with leishmania braziliensis braziliensis. the parasite was isolated from a lesion located on a castration scar, and identified by means of monoclonal antibodies. | 1986 | 3587004 |
| [cutaneous leishmaniasis in a mule (equus caballus x equus asinus) in an endemic area of the state of rio de janeiro]. | | 1986 | 3587005 |
| overwintering of non-migrating strongyles in donkeys in the highveld of zimbabwe. | three two-year-old male donkeys from a communal area in the highveld of zimbabwe were necropsied in july, the middle of the dry season. the strongylid populations of the three animals were very similar. approximately 80 per cent of these populations consisted of adult worms and only just over 10 per cent were early third stage larvae. the most common species were cylicostephanus minutus, cyathostomum montgomeryi, cylicocyclus nassatus and cylicocyclus auriculatus. nine other species of non-migra ... | 1987 | 3589174 |
| the application of a simple ria technique for the detection of 19-oh f1 alpha/f2 alpha prostaglandin, a specific semen marker, in semen contaminated vaginal swabs: time since intercourse studies. | the specificity of the 19-oh f1 alpha/f2 alpha prostaglandin antisera for the detection of semen in seminal/vaginal mixtures, has been evaluated. using a parallel curve test we found that the antibody showed a high specificity for these seminal prostaglandins in seminal/vaginal mixtures at concentrations of between 2 pg and 40 pg/100 microliter. the precision of the assay has been improved by the use of a donkey-anti-rabbit ferritin-bound second antibody. the application of this detection system ... | 1987 | 3596402 |
| successful transfer of the embryos of przewalski's horses (equus przewalskii) and grant's zebra (e. burchelli) to domestic mares (e. caballus). | blastocysts were collected non-surgically from 2 przewalski's horse and 2 grant's zebra mares and transferred extra-specifically to domestic horse and donkey recipients. nine przewalski's horse embryos were transferred surgically, and 2 non-surgically, to domestic welsh-type pony mares. after surgical transfer, 7 (77.8%) pregnancies were established and 4 foals were born. twelve grant's zebra embryos were transferred surgically to 5 pony and 7 domestic donkey recipients respectively and 1 non-su ... | 1987 | 3598950 |
| tick infestations on livestock in the yemen arab republic and their potential as vectors of livestock diseases. | a survey of ticks on cattle, camels, sheep, goats and donkeys in four different geographical locations of the yemen arab republic (yar) was carried out to provide more information on the possible risk of tick-borne diseases to imported exotic cattle included in the yar's plans for livestock development. the most abundant ticks were hyalomma spp. particularly on camels. ticks found on cattle included hyalomma spp., amblyomma variegatum, boophilus annulatus and rhipicephalus spp. in general with t ... | 1987 | 3603710 |
| liquid and solid two-phase radioimmunoassay--simultaneous measurement of levels of triiodothyronine and thyroxine of human serum in a single specimen. | the principle of the liquid and solid two-phase radioimmunoassay and its application to measuring the concentrations of triiodothyronine and thyroxine of human serum in a single sample at the same time are described in this paper. rabbit anti-triiodothyronine antiserum was immobilized on the inner surface of a plastic tube as solid-phase radioimmunoassay system of testing triiodothyronine and donkey anti-rabbit gamma globulin antiserum was used to separate immunocomplex in liquid-phase radioimmu ... | 1987 | 3616602 |
| anaerobic bacteria associated with dental abscesses in the horse and donkey. | | 1987 | 3622470 |
| games nurses play. part 4. pin the tail on the donkey and war. | | 1986 | 3639697 |
| an anatomical and endoscopic study of the nasopharynx and larynx of the donkey (equus asinus). | using histological techniques, anatomical dissection, fibre optic endoscopy and radiography, the nasopharynx and larynx of the donkey were examined and described. compared with horses, donkeys have a much deeper pharyngeal recess extending 4.0-6.0 cm caudally from a constricted orifice, through which it communicates with the nasopharynx. movements of the membranous lining of the recess reflect pressure changes in the upper airways. a shallow, thumb shaped depression on the rostral face of each v ... | 1986 | 3693039 |
| fungi on the hair of large mammals in egypt. | the frequency of occurrence of fungi in 260 hair samples of dog, donkey and cow collected from veterinary medicine hospital of assiut university was determined. twenty-three species which belong to fifteen genera were collected. in low frequency, several dermatophytes (chrysosporium, microsporum, arthroderma and trichophyton) were found as well as some other fungal species pathogenic to man and animals (aspergillus fumigatus, stachybotrys chartarum, scopulariopsis brevicaulis and cephalosporium ... | 1986 | 3713793 |
| quantitation of serum amyloid p component by an enzyme-linked immunoassay. | a solid phase enzyme-linked immunoassay (elisa) for the murine acute-phase reactant, serum amyloid p component (sap), was developed. the assay is based on our finding of a calcium-dependent binding of sap to trinitrophenyl-conjugated proteins. the wells of polystyrene microtiter plates are coated with trinitrophenylated keyhole limpet hemocyanin (tnp-klh), then incubated with sap-containing samples. the amount of sap is determined by indirect elisa, wells being sequentially incubated with rabbit ... | 1986 | 3722822 |
| citrullinemia: quantitative deficiency of argininosuccinate synthetase in the liver. | two cases of citrullinemia were reported. case 1 was an one month old female. her clinical course and findings were different from the fulminant type of neonatal citrullinemia reported in predominantly caucasian countries. our patient was well controlled under a low protein diet and essential amino acids till 9 months of age, but unfortunately she died of reye's like syndrome. case 2 was 31 year old male (at the time of death). he was admitted to our hospital because of hyperammonemia and mental ... | 1986 | 3738904 |
| molecular determinants of alphavirus neurovirulence: nucleotide and deduced protein sequence changes during attenuation of venezuelan equine encephalitis virus. | the nucleotide and deduced amino acid sequences of the structural proteins of the tc-83 vaccine strain of venezuelan equine encephalitis (vee) virus have been determined from a cdna clone containing the 26s mrna coding region. a cdna clone encoding the equivalent region of the virulent parent vee virus [trinidad donkey strain (trd)] has been sequenced previously. comparison of the sequences of the tc-83 and trd cdna clones revealed 13 nucleotide differences. neither the organization of the struc ... | 1986 | 3755750 |