| experimental susceptibility of gilthead sea bream, sparus aurata, via challenge with anisakis pegreffii larvae. | the endoscopic and histopathological findings detected in sparus aurata experimentally infected with third-stage anisakis larvae without intermediate host are evaluated and discussed. in six fish, live nematode larvae were introduced by gastroscopy into the stomach. the first observation by endoscope, 15 days after challenge, showed the presence of some larvae at the level of gastric mucosa. an explorative celioscopy, performed 60 days after challenge, showed haemorrhages and/or nodules on the g ... | 2013 | 23957000 |
| quality and antioxidant response of gilthead seabream (sparus aurata l.) to dietary supplements of fenugreek (trigonella foenum graecum) alone or combined with probiotic strains. | the present study was conducted to determine the potential effect of the dietary intake of fenugreek (trigonella foenum graecum) seeds alone or in combination with bacillus licheniformis, lactobacillus plantarum or b. subtilis on gilthead seabream quality and antioxidant response after 2 and 3 weeks of experimental feeding. the results showed that the supplements did not affect the percentage of the fatty acid profiles of muscle, demonstrating that all the additives tested can be administrated w ... | 2017 | 28232283 |
| effects of dietary administration of fenugreek seeds, alone or in combination with probiotics, on growth performance parameters, humoral immune response and gene expression of gilthead seabream (sparus aurata l.). | the use of immunostimulants is considered a promising preventive practice that may help to maintain animal welfare and a healthy environment, while increasing production and providing higher profits. the purpose of this study was to evaluate the effects on gilthead seabream (sparus aurata l.) of the dietary administration of fenugreek (trigonella foenum graecum) seeds, alone or combined with one of the following probiotic strains: bacillus licheniformis (tsb27), lactobacillus plantarum or bacill ... | 2017 | 27856325 |
| comparative analysis of the humoral immunity of skin mucus from several marine teleost fish. | fish skin mucus contains several immune substances that provide the first line of defence against a broad spectrum of pathogens although they are poorly studied to date. terminal carbohydrate composition and levels of total igm antibodies, several immune-related enzymes (lysozyme, peroxidase, alkaline phosphatase, esterases, proteases and antiproteases) as well as the bactericidal activity (against fish pathogenic vibrio harveyi, vibrio angillarum, photobacterium damselae and non-pathogenic bact ... | 2014 | 24972341 |
| comparative skin mucus and serum humoral defence mechanisms in the teleost gilthead seabream (sparus aurata). | mucosal surfaces of fish, including skin, gill and gut, contain numerous immune substances poorly studied that act as the first line of defence against a broad spectrum of pathogens. this study aimed to identify and characterize for the first time different constitutive humoral defence mechanisms of the skin mucus of gilthead seabream (sparus aurata). to do this, the levels of total immunoglobulin m, several enzymes and proteins (peroxidase, lysozyme, alkaline phosphatase, esterases, proteases a ... | 2014 | 24412437 |
| changes in intestinal morphology and microbiota caused by dietary administration of inulin and bacillus subtilis in gilthead sea bream (sparus aurata l.) specimens. | changes produced in gilthead sea bream (sparus aurata l.) intestinal morphology and microbiota caused by dietary administration of inulin and bacillus subtilis have been studied. gilthead sea bream specimens were fed diets containing 0 (control), inulin (10 g kg(-1)), b. subtilis (10(7) cfu g(-1)), or b. subtilis + inulin (10(7) cfu g(-1) + 10 g kg(-1)) for four weeks. curiously, fish fed the experimental diets (inulin, b. subtilis, or b. subtilis + inulin) showed the same morphological alterati ... | 2013 | 23403157 |
| effects of dietary inulin, bacillus subtilis and microalgae on intestinal gene expression in gilthead seabream (sparus aurata l.). | the present work describes effects of dietary inulin, two microalgae (tetraselmis chuii and phaeodactylum tricornutum) and bacillus subtilis (solely or combined with inulin or microalgae) on the expression of different genes in the intestine of the gilthead seabream (sparus aurata l.) following four weeks of a feeding trial. selected genes were grouped into five categories: genes involved in inflammation (genes encoding proinflammatory proteins), genes related to the cytoskeleton, genes encoding ... | 2013 | 23318995 |
| histological alterations and microbial ecology of the intestine in gilthead seabream (sparus aurata l.) fed dietary probiotics and microalgae. | the effects on histology and microbial ecology in gilthead seabream (sparus aurata) intestine caused by dietary probiotic and microalgae were studied. fish were fed non-supplemented (c, control) or supplemented diets with tetraselmis chuii, phaeodactylum tricornutum and bacillus subtilis single or combined (diets t, p, b, bt and bp) for 4 weeks. curiously, fish fed the experimental diets showed similar morphological alterations when studied by light and electron microscopy and significant signs ... | 2012 | 23053048 |
| effects of dietary bacillus subtilis, tetraselmis chuii, and phaeodactylum tricornutum, singularly or in combination, on the immune response and disease resistance of sea bream (sparus aurata l.). | combined or individual effects of two microalgae (phaeodactylum tricornutum and tetraselmis chuii) and bacillus subtilis on immune response, gene expression, and survival to challenge with photobacterium damselae subsp. piscicida of gilthead sea bream were investigated. to test the capacity of b. subtilis to grow employing the microalgae polysaccharides as energy and carbon source, an in vitro assay was defined, and demonstrated that the digestion product of microalgae, mainly p. tricornutum, su ... | 2012 | 22634255 |
| increases in immune parameters by inulin and bacillus subtilis dietary administration to gilthead seabream (sparus aurata l.) did not correlate with disease resistance to photobacterium damselae. | the present work evaluates the effects of inulin and bacillus subtilis, single or combined, on immune parameters, immune-related gene expression and protection against photobacterium damselae subsp. piscicida in gilthead seabream (sparus aurata). three trials were conducted. in the first trial, different concentrations of inulin (10, 15 and 30 g kg(-1)) (as a prebiotic) were administered to determine the optimal concentration for stimulating the seabream's immune system. in the second trial, the ... | 2012 | 22554575 |
| effects of dietary inulin and heat-inactivated bacillus subtilis on gilthead seabream (sparus aurata l.) innate immune parameters. | in the present study, a feeding trial was conducted to evaluate the effect of inulin and heat-inactivated bacillus subtilis, single or combined, on several innate immune activities of gilthead seabream (sparus aurata). forty-eight specimens were randomly assigned to four dietary treatments: 0 (control), inulin (10 g/kg, prebiotic group), b. subtilis (10(7) cfu/g, probiotic group), or b. subtilis + inulin (10(7) cfu/g + 10 g/kg, synbiotic group). after two and four weeks, six fish of each group w ... | 2012 | 22348912 |
| cardicola langeli sp. n. (digenea: aporocotylidae) from heart of sheepshead, archosargus probatocephalus (actinopterygii: sparidae) in the gulf of mexico, with an updated list of hosts, infection sites and localities for cardicola spp. | cardicola langeli n. sp. (digenea: aporocotylidae) infects the heart of sheepshead, archosargus probatocephalus (walbaum, 1792) (perciformes: sparidae) in the northern gulf of mexico off horn island (type locality), mississippi, usa. the new species is described herein using light and scanning electron microscopy of adult specimens and can be most easily distinguished from the other 24 accepted species of cardicola short, 1953 by the combination of having (i) an ovovitelline duct that extends an ... | 2013 | 23539948 |
| molecular characterization and expression analysis of toll-like receptor 2 in response to bacteria in silvery pomfret intestinal epithelial cells. | toll-like receptor 2 (tlr2) has been shown to play a crucial role in the host defense of pathogenic microbes in innate immunity. in this study, the full-length cdna of tlr2 in silvery pomfret (pampus argenteus) was cloned by homology cloning and the rapid amplification of cdna ends (race) technique. the complete cdna sequence of tlr2 was 2932 bp, containing an open reading frame (orf) of 2469 bp encoding 822 amino acids. a multiple alignment analysis of the silvery pomfret tlr2 protein-coding se ... | 2016 | 27574826 |
| experimental transmission of cryptosporidium molnari (apicomplexa: coccidia) to gilthead sea bream (sparus aurata l.) and european sea bass (dicentrarchus labrax l.). | cryptosporidium molnari was experimentally transmitted to gilthead sea bream (sparus aurata) and european sea bass (dicentrachus labrax) by oral infection with infected stomach scrapings. the infection was also cross-transmitted from infected gilthead sea bream to sea bass by cohabitation. the course of the infection was assessed after necropsy by three microscopic diagnostic methods and their sensitivity was compared. at the end of all the experiments the prevalence of infection reached 100%. i ... | 2003 | 12923629 |
| mitochondrial peroxiredoxin 3 (prx3) from rock bream (oplegnathus fasciatus): immune responses and role of recombinant prx3 in protecting cells from hydrogen peroxide induced oxidative stress. | pathogenic infections and environmental factors cause a variety of stresses in fish including oxidative stress by rapid elevation of reactive oxygen species (ros) and reactive nitrogen species (rns). transcriptional activation and expression of antioxidant enzymes are essential for reducing the oxidative stress. in this study, we present the molecular characterization, immune responses and ros scavenging activity of mitochondrial peroxiredoxin 3 from oplegnathus fasciatus (rbprx3). coding sequen ... | 2015 | 25542382 |
| detection of vana-containing enterococcus species in faecal microbiota of gilthead seabream (sparus aurata). | vancomycin-resistant enterococcus faecalis, e. faecium and e. durans isolates with the genotype vana were detected in 7 of 118 faecal samples (5.9%) of natural gilthead seabream recovered off the coast of portugal, and one vancomycin-resistant isolate/sample was further characterized. the genes erm(b), tet(l), tet(m), aac(6')-aph(2"), aph(3')-iiia and/or ant(6)-ia were identified in most of the 7 vancomycin-resistant enterococci. sequence types st273, st313 and st76 were detected in three e. fae ... | 2012 | 22641152 |
| a new intranuclear microsporidium, enterospora nucleophila n. sp., causing an emaciative syndrome in a piscine host (sparus aurata), prompts the redescription of the family enterocytozoonidae. | the presence of a new microsporidium is believed to be responsible for an emaciative syndrome observed in farmed gilthead sea bream (sparus aurata) from different facilities along the spanish coast. infected fish were approximately half the average weight and significant mortality was attributed to the condition in some facilities. clinical signs included anorexia, cachexia and pale internal organs. the microsporidium was found mainly in the intestinal mucosa and occasionally in the submucosa. m ... | 2014 | 24326177 |
| post-treatment of biologically treated wastewater containing organic contaminants using a sequence of h2o2 based advanced oxidation processes: photolysis and catalytic wet oxidation. | in this paper the feasibility of a multi-barrier treatment (mbt) for the regeneration of synthetic industrial wastewater (siww) was evaluated. industrial pollutants (orange ii, phenol, 4-chlorophenol and phenanthrene) were added to the effluent of municipal wastewater treatment plant. the proposed mbt begins with a microfiltration membrane pretreatment (mf), followed by hydrogen peroxide photolysis (h2o2/uvc) and finishing, as a polishing step, with catalytic wet peroxide oxidation (cwpo) using ... | 2015 | 25600300 |
| antibacterial and anti-paf activity of lipid extracts from sea bass (dicentrarchus labrax) and gilthead sea bream (sparus aurata). | the anti-paf and the antibacterial activities of lipid extracts obtained from cultured sea bass (dicentrarchus labrax) and cultured gilthead sea bream (sparus aurata) were evaluated. total lipids of sea bass and gilthead sea bream exerted paf-like activity while, in higher amounts they inhibited this paf activity. neutral lipids of both sea bass and gilthead sea bream contained only paf antagonists while the polar lipid fractions contained both paf antagonists and agonists. total lipids of sea b ... | 2008 | 26047447 |
| preparation of recombinant gilthead seabream (sparus aurata) growth hormone and its use for stimulation of larvae growth by oral administration. | gilthead seabream (sparus aurata) growth hormone (gsgh) cdna coding for the mature protein was cloned in a pgem-t vector and then transferred into prokaryotic expression vector pet-8 and expressed in e. coli bl21 (de3) cells upon induction with iptg. the expressed protein, contained within the inclusion-body pellet, was solubilized in 4.5 m urea, refolded at ph 11.3 in the presence of catalytic amounts of cysteine, and purified to over 98% purity, as evidenced by sds-page. gel-filtration on a su ... | 1999 | 9882554 |
| synergistic effects in the antiviral activity of the three mx proteins from gilthead seabream (sparus aurata). | due to their direct antiviral activity, mx proteins play a main role in the response mediated by type i interferon against viral infections. the study on gilthead seabream mx proteins is especially interesting, since this species is unusually resistant to viral diseases, being asymptomatic carrier of several viruses pathogenic to other fish species. gilthead seabream has three mx proteins (mx1, mx2 and mx3) that, separately, display antiviral activity against a wide range of viruses, showing int ... | 2015 | 26319936 |
| mx1, mx2 and mx3 proteins from the gilthead seabream (sparus aurata) show in vitro antiviral activity against rna and dna viruses. | mx proteins are important components of the antiviral innate immune response mediated by type i interferon. classically, these proteins have been considered to be triggered by viral rna, thus showing activity against rna viruses. actually, three mx proteins (saumx1, saumx2 and saumx3) from gilthead seabream (sparus aurata) have previously shown antiviral activity against a dsrna virus: the infectious pancreatic necrosis virus (ipnv) in vitro. for further characterizing their antiviral spectrum, ... | 2013 | 23911421 |
| a monogenean fish parasite, gyrodactylus chileani n. sp., belonging to a novel marine species lineage found in the south-eastern pacific and the mediterranean and north seas. | gyrodactylus chileani n. sp. is the first gyrodactylus species reported from chile. it is an ectoparasite living on fins and skin of a small fish, the chilean tidal pond dweller helcogrammoides chilensis (cancino) (perciformes: tripterygiidae). a phylogenetic analysis based on 5.8s+its2 of rdna placed the new species close to marine gyrodactylus species found in europe: g. orecchiae paladini, cable, fioravanti, faria, cave & shinn, 2009 on gilthead seabream sparus aurata l. from the adriatic and ... | 2012 | 22983803 |
| molecular identification of hysterothylacium aduncum specimens isolated from commercially important fish species of eastern mediterranean sea using mtdna cox1 and its rdna gene sequences. | the presence of a raphidascarid parasitic nematode hysterothylacium aduncum (rudolphi, 1802) in two sparid fish (sparus aurata and diplodus vulgaris) and one soleid fish (solea solea) was investigated in this study. a total of 868 individuals; 385 s. aurata, 437 d. vulgaris and 46 s. solea were collected from the mersin bay between february 2013 and january 2014 and examined. variations in the prevalence, mean intensity, and mean abundance of the parasite were 14.55%, 2.05, and 0.30 for s. aurat ... | 2015 | 25543079 |
| gilthead seabream (sparus aurata) mx gene promoters respond differentially to ipnv and vhsv infections in rtg-2 cells. | the understanding of virus-host interactions relies on the knowledge of the regulatory mechanisms of the type i interferon (ifn i)-stimulated genes (isgs). among isgs, those coding mx proteins play a main role due to their direct antiviral activity. the study of these genes in gilthead seabream is interesting, since this species displays high natural resistance to viral diseases, being asymptomatic carrier of infectious pancreatic necrosis virus (ipnv) and viral haemorrhagic septicaemia virus (v ... | 2016 | 26964720 |
| dietary administration of microalgae navicula sp. affects immune status and gene expression of gilthead seabream (sparus aurata). | effects of silage microalgae enriched with a probiotic and lyophilized microalgae were evaluated on main immune parameters and different gene expression of gilthead seabream (sparus aurata l.). a total of 60 seabream were grouped into 3 treatment diets which were a control diet (commercial diet) without microalgae (c), commercial diet supplemented with silage microalgae navicula sp. plus lactobacillus sakei 5-4 (10(6) cfu g(-1)) (sm), and commercial diet supplemented with lyophilized microalgae ... | 2013 | 23831495 |
| population dynamics of two diplectanid species (monogenea) parasitising sparid hosts (sparidae). | economically important sparid fish species, gilthead (sparus aurata) and white seabream (diplodus sargus) (sparidae) are frequently parasitised by diplectanid monogeneans, known to induce severe losses in farming conditions. we have analysed population dynamic of two diplectanid species, lamellodiscus echeneis and lamellodiscus ignoratus (monogenea: diplectanidae) collected from two bream species in the beymelek lagoon (southwest coast of turkey), comparing it between different host variables (f ... | 2015 | 25563608 |
| development of a convenience and safety chilled sous vide fish dish: diversification of aquacultural products. | the dynamic expansion of the ready-to-eat seabream sector in its adaptation to new lifestyles has led to the search for new presentation formats in seabream (sparus aurata). green sauce (olive oil, wine vinegar, garlic, fresh parsley, black pepper, basil and salt) and 60 ℃ of cooking temperature were chosen by the panellists for the sous vide cooking process. seabream fillet and sauce were packaged in polypropylene trays, cooked, chilled and stored at 2 ℃. microbiological (total viable counts,en ... | 2016 | 25941212 |
| survival of listeria monocytogenes and salmonella enteritidis in sea bream (sparus aurata) fillets packaged under enriched co2 modified atmospheres. | the effect of packaging of fresh sea bream fillets under different modified atmospheres (high levels of co2 combined with n2) on the behaviour of listeria monocytogenes and salmonella enteritidis was studied. two different strains of each pathogen were spread over the sea bream fillets stored at either 0 °c or 4 °c for 16 days under standard supermarket lighting conditions (14 h a day). the atmospheres investigated were 60% co2/40% n2, 70% co2/30% n2, 80% co2/20% n2 and air as control. mesophili ... | 2013 | 23454810 |
| characterization of the gilthead seabream (sparus aurata l.) immune response under a natural lymphocystis disease virus outbreak. | lymphocystis or lymphocystis disease virus (lcdv) is distributed worldwide and affects many fresh and marine water fish species. lcdv is commonly found in aquaria fish species but also in farmed fish species, among them the gilthead seabream (sparus aurata l.). the immune status of gilthead seabream (s. aurata) specimens under a natural outbreak of lcdv was studied. the replication of the virus was demonstrated in infected fish, but not in control fish. the results showed decreased total serum i ... | 2016 | 27133966 |
| evaluation of an integrated cell culture rt-pcr assay to detect and quantify infectious lymphocystis disease virus. | the lymphocystis disease virus (lcdv), a member of the iridoviridae family, infects a wide range of fish species including gilthead seabream (sparus aurata l.), the most important species cultured in the mediterranean. lcdv is difficult to propagate in cell culture and does not produce clear and consistent cytopathic effects (cpe), especially in samples collected from subclinically infected fish. an integrated cell culture reverse transcription-polymerase chain reaction (icc-rt-pcr) assay, follo ... | 2016 | 27756548 |
| rapid and sensitive detection of lymphocystis disease virus genotype vii by loop-mediated isothermal amplification. | lymphocystis disease virus (lcdv) infections have been described in gilthead seabream (sparus aurata l.) and senegalese sole (solea senegalensis, kaup), two of the most important marine fish species in the mediterranean aquaculture. in this study, a rapid, specific, and sensitive detection method for lcdv genotype vii based on loop-mediated isothermal amplification (lamp) was developed. the lamp assay, performed using an apparatus with real-time amplification monitoring, was able to specifically ... | 2017 | 27709436 |
| concurrence of iridovirus, polyomavirus, and a unique member of a new group of fish papillomaviruses in lymphocystis disease-affected gilthead sea bream. | lymphocystis disease is a geographically widespread disease affecting more than 150 different species of marine and freshwater fish. the disease, provoked by the iridovirus lymphocystis disease virus (lcdv), is characterized by the appearance of papillomalike lesions on the skin of affected animals that usually self-resolve over time. development of the disease is usually associated with several environmental factors and, more frequently, with stress conditions provoked by the intensive culture ... | 2016 | 27440877 |
| application of a new real-time polymerase chain reaction assay for surveillance studies of lymphocystis disease virus in farmed gilthead seabream. | lymphocystis disease (lcd) is the main viral infection reported to affect cultured gilthead seabream (sparus aurata) in europe. the existence of subclinical lymphocystis disease virus (lcdv) infection in this fish species has been recognised by using polymerase chain reaction (pcr)-based methods. nevertheless, these methods do not provide quantitative results that can be useful in epidemiological and pathological studies. moreover, carrier fish have been involved in viral transmission, therefore ... | 2016 | 27048523 |
| development and application of a real-time pcr assay for the detection and quantitation of lymphocystis disease virus. | lymphocystis disease virus (lcdv) is responsible for a chronic self-limiting disease that affects more than 125 teleosts. viral isolation of lcdv is difficult, time-consuming and often ineffective; the development of a rapid and specific tool to detect and quantify lcdv is desirable for both diagnosis and pathogenic studies. in this study, a quantitative real-time pcr (qpcr) assay was developed using a sybr-green-based assay targeting a highly conserved region of the mcp gene. primers were desig ... | 2015 | 25522921 |
| transmission of lymphocystis disease virus to cultured gilthead seabream, sparus aurata l., larvae. | the transmission of lymphocystis disease virus (lcdv) to gilthead seabream, sparus aurata l., larvae was investigated using fertilized eggs from a farm with previous reports of lymphocystis disease. lcdv genome was detected by pcr-hybridization in blood samples from 17.5% of the asymptomatic gilthead seabream broodstock analysed. using the same methodology, eggs spawned from these animals were lcdv positive, as well as larvae hatched from them. the presence of infective viral particles was confi ... | 2013 | 23163555 |
| morphological and phylogenetic description of a new xenoma-inducing microsporidian, microsporidium aurata nov. sp., parasite of the gilthead seabream sparus aurata from the red sea. | a new species of microsporidia found in the marine teleost sparus aurata collected from hurghada coasts along the red sea, egypt was described based on light and ultrastructural studies. twenty three (30.6%) out of 75 of the examined fish were parasitized with a microsporidian parasite. numerous macroscopic whitish cysts embedded in the peritoneal cavity were observed to infect many organs of the body including muscles, connective tissues, and the intestinal epithelium. the infection was develop ... | 2013 | 23990046 |
| algerian propolis extracts: chemical composition, bactericidal activity and in vitro effects on gilthead seabream innate immune responses. | propolis has been used as a medicinal agent for centuries. the chemical composition of four propolis samples collected from four locations of the sétif region, algeria, using gas chromatography-mass spectrometry was determined. more than 20 compounds and from 30 to 35 compounds were identified in the aqueous and ethanolic extracts, respectively. furthermore, the antimicrobial activity of the propolis extracts against two marine pathogenic bacteria was evaluated. finally, the in vitro effects of ... | 2017 | 28089892 |
| differential modulation of igt and igm upon parasitic, bacterial, viral, and dietary challenges in a perciform fish. | three different immunoglobulin (ig) isotypes can be found in teleost fish, igm, igd, and the teleost-specific igt. igm is considered to have a systemic activity, and igt is attributed a mucosal role, similar to mammalian iga. in this study, the complete sequence of gilthead sea bream igm and igt in their membrane (m) and soluble (s) forms are described for the first time in a perciform fish. their constitutive gene expression is analyzed in different tissues, and their regulation upon viral, bac ... | 2016 | 28082977 |
| genomic prediction of resistance to pasteurellosis in gilthead sea bream (sparus aurata) using 2b-rad sequencing. | gilthead sea bream (sparus aurata) is a species of paramount importance to the mediterranean aquaculture industry, with an annual production exceeding 140,000 metric tonnes. pasteurellosis due to the gram-negative bacterium photobacterium damselae subsp. piscicida (phdp) causes significant mortality, especially during larval and juvenile stages, and poses a serious threat to bream production. selective breeding for improved resistance to pasteurellosis is a promising avenue for disease control, ... | 2016 | 27652890 |
| transcription of histones h1 and h2b is regulated by several immune stimuli in gilthead seabream and european sea bass. | histones (h1 to h4) are the primary proteins which mediate the folding of dna into chromatin; however, and in addition to this function, histones have been also related to antimicrobial peptides (amps) activity in vertebrates, in fact, mammalian h1 is mobilized as part as the anti-viral immune response. in fish, histones with amp activity have been isolated and characterized mainly from skin and gonads. one of most threatening pathogens for wild and cultured fish species nowadays is nodavirus (n ... | 2016 | 27523279 |
| pathogenesis of photobacterium damselae subspecies infections in sea bass and sea bream. | photobacterium damselae is a gram negative bacterium causes photobacteriosis, a worldwide septicemic disease in aquaculture including sea bass (dicentrarchus labrax) and sea bream (sparus aurata). the pathogenicity of bacterial subspecies and the disease pathological changes in natural and experimental infections have thus far yielded inconsistency of effective preventive measures. this study aimed to represent a comprehensive analysis of the potential pathogenic capacities of the two subspecies ... | 2016 | 27497892 |
| in vitro cytokine profile revealed differences from dorsal and ventral skin susceptibility to pathogen-probiotic interaction in gilthead seabream. | skin is the first barrier of defense on fish, which is crucial to protection against different stressors, including pathogens. skin samples obtained from dorsal and ventral part of sparus aurata specimens were incubated with photobacterium damselae subsp. piscicida (a pathogen for this fish species), with shewanella putrefaciens pdp11 (a probiotic bacteria isolated from healthy gilthead seabream skin) or with both bacteria. the gene expression profile of nine cytokines (il1b, tnfa, il6, il7, il8 ... | 2016 | 27422755 |
| immunization of sea bream (sparus aurata) juveniles against photobacterium damselae subsp. piscicida by short bath: effect on some pro-inflammatory molecules and the mx gene expression. | cytokines are a family of proteins derived from macrophages, lymphocytes, granulocytes, mast cells and epithelial cells and can be divided into interferons (ifns), interleukins (ils) and tumor necrosis factors (tnfs) among others. the presence of cytokines in a wide number of fish species has been proved and several molecules types have been already cloned and sequenced. in this work some proinflamatory molecules and mx gene were detected in the liver of vaccinated sea bream juveniles with an av ... | 2015 | 26118933 |
| virulence properties, biofilm formation and random amplified polymorphic dna analysis of photobacterium damselae subsp. damselae isolates from cultured sea bream (sparus aurata) and sea bass (dicentrarchus labrax). | photobacterium damselae subsp. damselae has been isolated from different outbreaks affecting cultured sparus aurata and dicentrarchus labrax. the aim of the present study was to characterize the phenotype and genotype of 12 p. damselae subsp. damselae strains isolated from these outbreaks. the roles of skin mucus in resistance to the initial infection steps have been studied. all tested strains resisted the bactericidal activity of the mucus and showed an ability to adhere to it, but only those ... | 2014 | 24685699 |
| high mortality of juvenile gilthead sea bream (sparus aurata) from photobacteriosis is associated with alternative macrophage activation and anti-inflammatory response: results of gene expression profiling of early responses in the head kidney. | the halophilic bacterium photobacterium damselae subsp. piscicida (phdp) represents a substantial health problem for several fish species in aquaculture. bacteria that reside free and inside phagocytes cause acute and chronic forms of photobacteriosis. infections of juveniles rapidly kill up to 90-100% fish. factors underlying failure of the immune protection against bacteria remain largely unknown. the reported study used a transcriptomic approach to address this issue. juvenile sea breams (0.5 ... | 2013 | 23485716 |
| immunostimulatory effects of prolactin on tlr1 and tlr5m in shk-1 cells infected with piscirickettsia salmonis. | the innate immune system is the first line of defense against infection by pathogens. it consists of various elements, including toll-like receptors (tlrs), which recognize molecular patterns associated with pathogens and trigger the immune response, through activation of important transcription factors such as nf-κb, which are usually found sequestered in the cytoplasm by iκbα until it receives the release signal. piscirickettsia salmonis causes piscirickettsiosis or salmonid rickettsial septic ... | 2016 | 27025311 |
| morphology and molecular analysis of life-cycle stages of proctoeces maculatus (looss, 1901) (digenea: fellodistomidae) in the bizerte lagoon, tunisia. | the life cycle of proctoeces maculatus (looss, 1901) (digenea, fellodistomidae) was studied in bizerte lagoon (tunisia). three sequential hosts appear to be involved: the mediterranean mussel mytilus galloprovincialis lamarck, 1819 (mytilidae) as the first intermediate host; the polychaete sabella pavonina savigny, 1822 (sabellidae), as the second intermediate host; and fishes (lithognathus mormyrus (linnaeus, 1758) (sparidae), trachinotus ovatus (linnaeus, 1758) (carangidae) and sparus aurata l ... | 2016 | 26694018 |
| molecular epidemiology of betanodaviruses isolated from sea bass and sea bream cultured along the tunisian coasts. | viral nervous necrosis (vnn) is a serious viral disease affecting farmed sea bass (dicentrarchus labrax). only scarce molecular data are available on the disease-causing betanodavirus populations in tunisia. therefore, we carried out the first molecular survey of betanodaviruses in farmed sea bass and sea bream (sparus aurata) along the tunisian coasts. among 81 samples from five farms, 20 tested positive with rt-pcr, not only in clinical cases but also in asymptomatic fish before and during out ... | 2013 | 23288625 |
| dietary yeast sterigmatomyces halophilus enhances mucosal immunity of gilthead seabream (sparus aurata l.). | a yeast was isolated from hypersaline sediments, grown and phylogenetically characterized as sterigmatomyces halophilus strainn16. the dietary administration of this yeast was studied for its effect on skin mucosal immune and antioxidant status of gilthead seabream (sparus aurata l.). fish were fed a commercial diet (control, non-supplemented diet), or the same commercial diet supplemented with 0.55% or 1.1% of yeast for 15 and 30 days. one month after the end of the trial, fish from all treatme ... | 2017 | 28315392 |
| structural and functional characterizations of activin type 2b receptor (acvr2b) ortholog from the marine fish, gilthead sea bream, sparus aurata: evidence for gene duplication of acvr2b in fish. | myostatin (mstn), a negative regulator of muscle growth and a member of the transforming growth factor-β superfamily, can bind the two activin type 2 receptors (acvr2). it has been previously shown that wt mice injected with acvr2b extracellular domain (acvr2b-ecd) had higher muscle mass. likewise, fish larvae immersed in pichia pastoris culture supernatant, containing goldfish acvr2b-ecd, showed enhanced larval growth. however, it is not clear whether fish mstn1 and mstn2 signal through the sam ... | 2012 | 22911153 |
| effects of shewanella putrefaciens on innate immunity and cytokine expression profile upon high stocking density of gilthead seabream specimens. | high stocking density increases the number of emerging diseases triggering economic losses worldwide. probiotics provide an effective and natural solution for preventing some diseases through an improvement of innate immune system among others. in the present work dietary administration of the probiotic shewanella putrefaciens (known as pdp11) was evaluated under stress by high stocking density after 2 and 4 weeks of administration to gilthead seabream (sparus aurata) specimens. results showed a ... | 2016 | 26879495 |
| enrichment of gilthead seabream (sparus aurata l.) diet with palm fruit extracts and probiotics: effects on skin mucosal immunity. | fish skin mucus contains numerous immune substances still poorly studied. to date, there are no studies regarding the possible influence of dietary supplements on such important substances. in the present work, a commercial diet used as control diet was enriched with: 1) probiotic shewanella putrefaciens (pdp11 diet, 10(9) cfu g(-1)); 2) probiotic bacillus sp. (bacillus diet, 10(9) ufc g(-1)); 3) aqueous date palm fruits extracts (dpe diet, 4%), and 4) a combination of pdp11 + bacillus sp + aque ... | 2016 | 26712151 |
| differential proteome profile of skin mucus of gilthead seabream (sparus aurata) after probiotic intake and/or overcrowding stress. | gilthead seabream (sparus aurata l.) is the major cultured fish species in the mediterranean area. high density stocking causes stress and increases the impact of diseases leading to economic losses. probiotics could represent a solution to prevent diseases through several mechanisms such as improving the immune status and/or mucosal microbiota or competing with pathogens. the probiotic shewanella putrefaciens, also known as pdp11, was firstly isolated from the skin of healthy gilthead seabream. ... | 2016 | 26617323 |
| modulation of immunity and gut microbiota after dietary administration of alginate encapsulated shewanella putrefaciens pdp11 to gilthead seabream (sparus aurata l.). | the potential benefits of probiotics when administering to fish could improve aquaculture production. the objective of this study was to examine the modulation of immune status and gut microbiota of gilthead seabream (sparus aurata l.) specimens by a probiotic when administered encapsulated. commercial diet was enriched with shewanella putrefaciens pdp11 (sppdp11, at a concentration of 10(8) cfu g(-1)) before being encapsulated in calcium alginate beads. fish were fed non-supplemented (control) ... | 2015 | 26003737 |
| the treatment with the probiotic shewanella putrefaciens pdp11 of specimens of solea senegalensis exposed to high stocking densities to enhance their resistance to disease. | aquaculture industry exposes fish to acute stress events, such as high stocking density, and a link between stress and higher susceptibility to diseases has been concluded. several studies have demonstrated increased stress tolerance of fish treated with probiotics, but the mechanisms involved have not been elucidated. shewanella putrefaciens pdp11 is a strain isolated from healthy gilthead seabream (sparus aurata l.) and it is considered as probiotics. the aim of this study was to evaluate the ... | 2014 | 25149590 |
| effect of dietary supplementation of probiotics and palm fruits extracts on the antioxidant enzyme gene expression in the mucosae of gilthead seabream (sparus aurata l.). | antioxidant activity is particularly important, since oxidation is an unavoidable reaction in all living bodies. at present, natural antioxidants to be used on food as an alternative to synthetic ones are being sought. gilthead seabream (sparus aurata l.) specimens were fed for 4 weeks with diets enriched with bacterial probiotics (shewanella putrefaciens pdp11 and bacillus sp), single or in combination with tunisian dates palm fruit extracts. the expression of the main antioxidant enzyme genes ... | 2014 | 24952087 |
| dietary administration of β-1,3/1,6-glucan and probiotic strain shewanella putrefaciens, single or combined, on gilthead seabream growth, immune responses and gene expression. | it is widely known that β-glucans and probiotic bacteria are good immunostimulants for fish. in the present work we have evaluated the dietary effect of β-1,3/1,6-glucan (isolated from laminarina digitata) and pdp 11 (shewanella putrefaciens, probiotic isolated from gilthead seabream skin), single or combined, on growth, humoural (seric level of total igm antibodies and peroxidase and antiprotease activities) and cellular innate immune response (peroxidase and phagocytic activities of head-kidne ... | 2014 | 24798993 |
| application of compound mixture of caprylic acid, iron and mannan oligosaccharide against sparicotyle chrysophrii (monogenea: polyopisthocotylea) in gilthead sea bream, sparus aurata. | we have evaluated the therapeutic effect of a compound mixture of caprylic acid (200 mg/kg fish), organic iron (0.2% of diet) and mannan oligosaccharide (0.4% of diet) in gilthead sea bream, sparus aurata linnaeus, infected with sparicotyle chrysophrii beneden et hesse, 1863 in controlled conditions. one hundred and ten reared and s. chrysophrii-free fish (197 g) located in a cement tank were infected by the parasite two weeks following the addition of 150 s. chrysophrii-infected fish (70 g). gr ... | 2016 | 27507773 |
| strong effect of long-term sparicotyle chrysophrii infection on the cellular and innate immune responses of gilthead sea bream, sparus aurata. | one thousand healthy recipient gilthead sea bream, sparus aurata, cohabited with 250 donor fish parasitized by sparicotyle chrysophrii (van beneden and hesse, 1963) (monogenea: polyopisthocotylea), a common parasite of the gills of this fish species. controls consisted of 1000 healthy fish kept in a separate tank. after 10 weeks, fish were weighed and parasite load, hemoglobin concentration and immunological parameters were assessed. rather than the absence of parasite, hemoglobin concentration ... | 2015 | 25825219 |
| risk assessment for parasites in cultures of diplodus puntazzo (sparidae) in the western mediterranean: prospects of cross infection with sparus aurata. | the sharpsnout seabream diplodus puntazzo is of interest in mediterranean fish farming. disease is an important problem because parasites can spread quickly in culture conditions and fish often develop high parasite burdens. here we assess the risk that documented parasites pose to the sustainability of d. puntazzo farming. this study specifically considers metazoan and protist parasites recorded from wild and farmed d. puntazzo in scientific literature. risk assessment studies involve the ident ... | 2014 | 24893693 |
| streptococcus iniae in gilthead seabream (sparus aurata, l.) and red porgy (pagrus pagrus, l.): ultrastructural analysis. | streptococcosis caused by streptococcus iniae has become one of the most serious marine and freshwater aquatic diseases in the past decade, causing large losses in farmed and wild fish worldwide. in this study, we performed an ultrastructural study of major lesions in gilthead seabream sparus aurata and red porgy pagrus pagrus experimentally infected with the s. iniae iusa-1 strain, isolated in a natural outbreak in spain in the mentioned species. the transmission electron micrographs revealed t ... | 2015 | 24496225 |
| pharmacokinetics and residue depletion of erythromycin in gilthead sea bream sparus aurata l. after oral administration. | erythromycin (ery) is an antibiotic effective against streptococcus iniae, a microorganism responsible for significant losses in aquaculture. no data are available on the pharmacokinetics and residue depletion of ery in sea bream. the aim of this study was thus to evaluate the pharmacokinetics of ery in this species after a single oral administration at 75 mg kg(-1) b.w. and to assess its residue depletion from tissues after prolonged treatment for 10 days. ery was rapidly absorbed in sea bream ... | 2014 | 23992037 |
| whole-genome sequence of the fish virulent strain streptococcus iniae iusa-1, isolated from gilthead sea bream (sparus aurata) and red porgy (pagrus pagrus). | streptococcus iniae is a major fish pathogen that produces invasive infections that result in economic losses in aquaculture. in this study, the draft genome sequence of streptococcus iniae strain iusa-1, isolated from a natural outbreak affecting gilthead sea bream (sparus aurata) and red porgy (pagrus pagrus), is presented. | 2013 | 23516181 |
| the in vitro effect of probiotic vagococcus fluvialis on the innate immune parameters of sparus aurata and dicentrarchus labrax. | in this study we evaluated the effect of the probiotic vagococcus fluvialis on the cellular immune unspecific system of two different fish species of great interest in aquaculture such as gilthead sea bream (sparus aurata) and european sea bass (dicentrarchus labrax). leucocytes from head kidney of the two fish species were extracted and concentration adjusted to 10(7) cells ml(-1). phagocytic and respiratory burst activity and the peroxidase content of leucocytes were observed 30 min after incu ... | 2012 | 22864109 |
| antibiotic resistance of vibrio species isolated from sparus aurata reared in italian mariculture. | extensive use of antimicrobial agents in finfish farming and the consequent selective pressure lead to the acquisition of antibiotic resistance in aquaculture environment bacteria. vibrio genus represents one of the main pathogens affecting gilthead sea bream. the development of antibiotic resistance by vibrio represents a potential threat to human health by exchange of resistant genes to human pathogens through food chain. the objective of the present study was to conduct a multisite survey on ... | 2014 | 25180847 |
| designation of pathogenic resistant bacteria in the sparusaurata sea collected in tunisia coastlines: correlation with high performance liquid chromatography-tandem mass spectrometry analysis of antibiotics. | vibrio is characterized by a large number of species and some of them are human pathogens causing gastro intestinal and wound infections through the ingestion or manipulation of contaminated fishes including vibrio parahaemolyticus and vibrio alginolyticus. in this study, we reported the phenotypic and molecular characterization of vibrio parahaemolyticus and vibrio alginolyticus strains isolated from wild and farm sea bream (sparus aurata l.) along the tunisian coast from december 2015 to april ... | 2017 | 28062288 |
| isolation and characterization of two lytic bacteriophages, φst2 and φgrn1; phage therapy application for biological control of vibrio alginolyticus in aquaculture live feeds. | bacterial infections are a serious problem in aquaculture since they can result in massive mortalities in farmed fish and invertebrates. vibriosis is one of the most common diseases in marine aquaculture hatcheries and its causative agents are bacteria of the genus vibrio mostly entering larval rearing water through live feeds, such as artemia and rotifers. the pathogenic vibrio alginolyticus strain v1, isolated during a vibriosis outbreak in cultured seabream, sparus aurata, was used as host to ... | 2016 | 26950336 |
| draft genome sequences of vibrio alginolyticus strains v1 and v2, opportunistic marine pathogens. | we announce the draft genome sequences of vibrio alginolyticus strains v1 and v2, isolated from juvenile sparus aurata and dentex dentex, respectively, during outbreaks of vibriosis. the genome sequences are 5,257,950 bp with a g+c content of 44.5% for v. alginolyticus v1 and 5,068,299 bp with a g+c content of 44.8% for strain v2. these genomes provide further insights into the putative virulence factors, prophage carriage, and evolution of this opportunistic marine pathogen. | 2015 | 26139724 |
| effect of lipopolysaccharides from vibrio alginolyticus on the mx gene expression and virus recovery from gilthead sea bream (sparus aurata l.) experimentally infected with nodavirus. | infections with nodavirus affect a wild and farmed fish species throughout the world, mostly from the marine environment. the aim of this work was to determine the immune status of gilthead sea bream that comes as a result of a nodavirus infection, induced by activation of the interferon response pathway by lipopolysaccharides from vibrio alginolyticus and the expression of interferoninduced mx protein in liver samples. the enhancement of mx protein gene expression was detected in liver samples ... | 2013 | 23092730 |
| phenotypic characterization and rapd fingerprinting of vibrio parahaemolyticus and vibrio alginolyticus isolated during tunisian fish farm outbreaks. | the genus vibrio is characterized by a large number of species and some of them are human pathogens causing gastrointestinal and wound infections through the ingestion or manipulation of contaminated fishes and shellfish including vibrio parahaemolyticus and vibrio alginolyticus. in this study, we reported the phenotypic and molecular characterization of 9 v. parahaemolyticus and 27 v. alginolyticus strains isolated from outbreaks affecting cultured gilthead sea bream (sparus aurata l.) and sea ... | 2013 | 22684973 |
| histamine and mast cell activator compound 48/80 are safe but inefficient systemic adjuvants for gilthead seabream vaccination. | histamine has a key role in the regulation of inflammatory and innate immune responses in vertebrates. gilthead seabream (sparus aurata l.), a marine hermaphrodite teleost of great commercial value, was the first fish species shown to possess histamine-containing mast cells (mcs) at mucosal tissues. mcs are highly abundant in the peritoneal exudate of gilthead seabream and compound 48/80 (co 48/80), often used to promote mc activation and histamine release, is able to promote histamine release f ... | 2017 | 28193449 |
| a synthetic peptide derived from the d1 domain of flagellin induced the expression of proinflammatory cytokines in fish macrophages. | flagellin is the main protein component of flagellum in gram negative and positive bacteria, and it is also the ligand that activates the toll-like receptor 5 (tlr5) in fish and mammals. in higher vertebrates, flagellin induces the activation of the membrane-bound tlr5 (tlr5m), which promotes the expression of proinflammatory cytokines and chemokines, and other immunological functions. we have previously reported that recombinant flagellin from vibrio anguillarum and its nd1 domain are able to u ... | 2015 | 26363237 |
| effects of recombinant flagellin b and its nd1 domain from vibrio anguillarum on macrophages from gilthead seabream (sparus aurata l.) and rainbow trout (oncorhynchus mykiss, w.). | flagellin is the principal component of flagellum in gram negative and positive bacteria, and it is also the ligand that activates the toll-like receptor 5 (tlr5) in mammals and fish. in higher vertebrates, flagellin induces the activation of the membrane-bound tlr5 (tlr5m), which promotes the expression of proinflammatory cytokines and chemokines and the co-stimulatory molecules present in antigen-presenting cells needed for the activation of t cells. in the present study, we report the product ... | 2015 | 25449380 |
| toll-like receptor 22 of gilthead seabream, sparus aurata: molecular cloning, expression profiles and post-transcriptional regulation. | tlr22 is a fish-specific tlr that recognizes dsrnas. in the present study, a tlr22 homologue gene from gilthead seabream (sbtlr22) was identified and characterized. the full coding sequence contained a single open-reading frame of 2895 nucleotides encoding a predicted protein of 964 amino acids in length. its 3'-utr was relatively long, 1380 nucleotides, and contained three au-rich sequences frequently associated with mrna instability. functional studies showed that the sbtlr22 transcript had a ... | 2014 | 24333435 |
| molecular cloning, phylogenetic analysis and functional characterization of soluble toll-like receptor 5 in gilthead seabream, sparus aurata. | two forms of tlr5, one membrane-anchored and one soluble, have been described in some teleost fish species. however, the exact role of each form has been poorly studied. in the present study, we show that the mrna levels of soluble gilthead seabream tlr5 (sbtlr5s) are highly induced in head kidney, spleen, liver and blood after vibrio anguillarum infection, suggesting an important role for sbtlr5s in the innate immune response against bacteria. comparative genomic and phylogenetic analyses revea ... | 2013 | 23571319 |
| characterization of european sea bass transcripts by rna seq after oral vaccine against v. anguillarum. | the european sea bass (dicentrarchus labrax) is, along with the gilthead sea bream (sparus aurata), one of the most extensively cultured species in european aquaculture productions. massive mortalities may be caused by bacterial or viral infections in intensive aquaculture production. evaluation of the efficacy of an oral vaccine against vibrio anguillarum (aquavac vibrio oral) in sea bass revealed specific immune gene expression profiles in the gut as well as protection of fish. in the present ... | 2012 | 22790793 |
| enhancing gilthead seabream immune status and protection against bacterial challenge by means of antigens derived from vibrio parahaemolyticus. | in an attempt to control the proliferation of the pathogenic bacterium vibrio parahaemolyticus in gilthead seabream (sparus aurata), the immunostimulant effect of lysate and toxa from this bacterium was evaluated. fish were intraperitoneally injected twice (first injection, day 1 of the experiment; second injection, day 7) and sampled after one week (on days 8 and 15). afterwards, all fish specimens were experimentally infected with v. parahaemolyticus and mortality was recovered for 1 week. fis ... | 2017 | 27890799 |
| vibrio renipiscarius sp. nov., isolated from cultured gilthead sea bream (sparus aurata). | two strains of gram-negative, facultatively anaerobic, slightly halophilic bacteria, isolated from healthy gilthead sea bream (sparus aurata) cultured in spanish mediterranean fish farms, were different from their closest relatives, vibrio scophthalmi and v. ichthyoenteri, by phenotypic, phylogenetic and genomic standards. the strains were negative for decarboxylase tests and lacked extracellular hydrolytic activities, but were able to ferment d-mannitol, sucrose, cellobiose and d-gluconate, amo ... | 2015 | 25795068 |
| flagellin from marinobacter algicola and vibrio vulnificus activates the innate immune response of gilthead seabream. | adjuvants have emerged as the best tools to enhance the efficacy of vaccination. however, the traditional adjuvants used in aquaculture may cause adverse alterations in fish making necessary the development of new adjuvants able to stimulate the immune system and offer strong protection against infectious pathogens with minimal undesirable effects. in this respect, flagellin seems an attractive candidate due to its ability to strongly stimulate the immune response of fish. in the present study, ... | 2014 | 25020195 |
| absence of viral hemorrhagic septicemia virus (vhsv) and infectious hemorrhagic necrosis virus (inhv) in a tunisian fish farm: a case study. | beyond the obvious problems related to the sustainable management of wild stocks, current fish farming practices in the mediterranean area entail important environmental risks and potential outbreaks of fish diseases linked to massive translocations across regional boundaries. viral haemorrhagic septicaemia (vhs) and infectious hematopoietic necrosis (ihn) are well-known fish diseases caused by the vhsv and ihnv viruses, and positive cases are subject to obligatory reporting to the world organis ... | 2012 | 24834660 |
| isolation of lacinutrix venerupis strains associated with disease outbreaks in sea bream sparus aurata and european sea bass dicentrarchus labrax. | four gram-negative bacterial isolates were recovered from 2 disease outbreaks that occurred in 2013 affecting european sea bass dicentrarchus labrax fry and sea bream sparus aurata adults. main symptoms were erratic swimming, eroded fins and, in the sea bream outbreak, haemorrhages on the body surface; bacteria were always recovered from internal organs, almost in pure culture. on the basis of phenotypic characterization and 16s rrna gene sequence analysis, the isolates were identified as lacinu ... | 2017 | 28357988 |
| a novel approach to limit chemical deterioration of gilthead sea bream (sparus aurata) fillets: coating with electrospun nanofibers as characterized by molecular, thermal, and microstructural properties. | coating of sea bream fillets with thymol loaded chitosan based electrospun nanofibers (tlcn) and chitosan based nanafibers (cn) has been presented a novel approach to delay chemical deterioration. we assessed cn and tlcn with respect of scanting of total volatile basic nitrogen (tvbn), trimethylamine (tma), thiobarbituric acid (tba) deterioration during cold storage condition. electrospinning process was applied to obtain tlcn and cn. both of nanofibers obtained from biopolymer and bioactive mat ... | 2017 | 28346739 |
| effects of graded dietary levels of soy protein concentrate supplemented with methionine and phosphate on the immune and antioxidant responses of gilthead sea bream (sparus aurata l.). | the effects of a dietary soy protein concentrate (spc) as a fish meal (fm) substitute, on selected innate immune responses, the oxidative status, hepatic and intestinal morphology of gilthead sea bream, sparus aurata, were evaluated after a three-month feeding trial. isonitrogenous (45% crude protein) and isoenergetic (23 kj/g gross energy) diets with 20% (spc20), 40% (spc40) and 60% (spc60) of spc inclusion, supplemented with methionine and phosphate, were evaluated against a diet containing fm ... | 2017 | 28284854 |
| molecular taxonomic profiling of bacterial communities in a gilthead seabream (sparus aurata) hatchery. | as wild fish stocks decline worldwide, land-based fish rearing is likely to be of increasing relevance to feeding future human generations. little is known about the structure and role of microbial communities in fish aquaculture, particularly at larval developmental stages where the fish microbiome develops and host animals are most susceptible to disease. we employed next-generation sequencing (ngs) of 16s rrna gene reads amplified from total community dna to reveal the structure of bacterial ... | 2017 | 28261166 |
| the effect of starvation and re-feeding on vasotocinergic and isotocinergic pathways in immature gilthead sea bream (sparus aurata). | this study describes the responses of the vasotocinergic and isotocinergic systems to food deprivation and re-feeding processes in immature gilthead sea bream (sparus aurata). the animals were subjected to the following experimental treatments: (1) normal feeding (control), (2) food deprivation for 21 days; and (3) re-feeding for 7 days, beginning 14 days after starvation. the animals were sampled at 0, 7, 14 and 21 days from the beginning of the trial. the pituitary and plasma arginine vasotoci ... | 2017 | 28251326 |
| olive oil bioactive compounds increase body weight, and improve gut health and integrity in gilthead sea bream (sparus aurata). | an olive oil bioactive extract (obe) rich in bioactive compounds like polyphenols, triterpenic acids, long-chain fatty alcohols, unsaturated hydrocarbons, tocopherols and sterols was tested (0, 0·08, 0·17, 0·42 and 0·73 % obe) in diets fed to sea bream (sparus aurata) (initial weight: 5·4 (sd 1·2) g) during a 90-d trial (four replicates). fish fed diets containing 0·17 and 0·42 % obe were 5 % heavier (61·1 (sd 1·6) and 60·3 (sd 1·1) g, respectively) than those of the control group (57·0 (sd 0·7) ... | 2017 | 28245885 |
| moderate and sustained exercise modulates muscle proteolytic and myogenic markers in gilthead sea bream (sparus aurata). | swimming activity primarily accelerates growth in fish by increasing protein synthesis and energy efficiency. the role of muscle in this process is remarkable and especially important in teleosts, where muscle represents a high percentage of body weight, and by the continuous growth that many fish species present. the aim of this work was to characterize the effects of five weeks of moderate and sustained swimming in gene and protein expression of myogenic regulatory factors, proliferation marke ... | 2017 | 28228414 |
| skin mucus of gilthead sea bream (sparus aurata l.). protein mapping and regulation in chronically stressed fish. | the skin mucus of gilthead sea bream was mapped by one-dimensional gel electrophoresis followed by liquid chromatography coupled to high resolution mass spectrometry using a quadrupole time-of-flight mass analyzer. more than 2,000 proteins were identified with a protein score filter of 30. the identified proteins were represented in 418 canonical pathways of the ingenuity pathway software. after filtering by canonical pathway overlapping, the retained proteins were clustered in three groups. the ... | 2017 | 28210224 |
| linking cortisol response with gene expression in fish exposed to gold nanoparticles. | fish exposure to environmental stressors (e.g. chemicals, hypoxia, temperature) induce responses enabling them to cope with alterations in their environment. a stress response involves a wide array of changes, from molecular to physiological and behavioural, set to counteract the effect of the stressor and recover homeostatic equilibrium. among other processes, there is activation of the hypothalamus-pituitary-interrenal (hpi) axis, resulting in stimulation of the steroidogenic pathway and relea ... | 2017 | 28169028 |
| untargeted metabolomics approach for unraveling robust biomarkers of nutritional status in fasted gilthead sea bream (sparus aurata). | a metabolomic study has been performed to identify sensitive and robust biomarkers of malnutrition in farmed fish, using gilthead sea bream (sparus aurata) as a model. the metabolomic fingerprinting of serum from fasted fish was assessed by means of ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. more than 15,000 different m/z ions were detected and partial least squares-discriminant analysis allowed a clear differentiation between the two exp ... | 2017 | 28168106 |
| extending immunological profiling in the gilthead sea bream, sparus aurata, by enriched cdna library analysis, microarray design and initial studies upon the inflammatory response to pamps. | this study describes the development and validation of an enriched oligonucleotide-microarray platform for sparus aurata (saq) to provide a platform for transcriptomic studies in this species. a transcriptome database was constructed by assembly of gilthead sea bream sequences derived from public repositories of mrna together with reads from a large collection of expressed sequence tags (est) from two extensive targeted cdna libraries characterizing mrna transcripts regulated by both bacterial a ... | 2017 | 28165358 |
| bioconcentration and biotransformation of amitriptyline in gilt-head bream. | extensive global use of the serotonin-norepinephrine reuptake inhibitor amitriptyline (ami) for treatment of mental health problems has led to its common occurrence in the aquatic environment. to assess ami bioconcentration factors, tissue distribution, and metabolite formation in fish, we exposed gilt-head bream (sparus aurata) to ami in seawater for 7 days at two concentrations (0.2 μg/l and 10 μg/l). day 7 proportional bioconcentration factors (bcfs) ranged from 6 (10 μg/l dose, muscle) to 12 ... | 2017 | 28106990 |
| anthropogenic chemical cues can alter the swimming behaviour of juvenile stages of a temperate fish. | human pressure on coastal areas is affecting essential ecosystems including fish nursery habitats. among these anthropogenic uses, the seasonal increment in the pressure due to leisure activities such as coastal tourism and yachting is an important environmental stressor in many coastal zones. these pressures may elicit understudied impacts due to, for example, sunscreens or other seasonal pollutants. the island of majorca, northwest mediterranean sea, experiences one of the highest number of to ... | 2017 | 28103523 |
| osmoregulatory role of vasotocinergic and isotocinergic systems in the gilthead sea bream (sparus aurata l). | gilthead sea bream, sparus aurata l., is an important fish species for the mediterranean aquaculture and is considered a good model for studying the osmoregulatory process, due to its capacity to cope with great changes in environmental salinity (5-60‰). our group studied the osmoregulatory role of different endocrine systems in this species, focusing on the vasotocinergic and isotocinergic systems over several years. for this purpose, the cdnas coding for pro-vasotocin (pro-vt), pro-isotocin (p ... | 2017 | 28065737 |
| dietary butyrate helps to restore the intestinal status of a marine teleost (sparus aurata) fed extreme diets low in fish meal and fish oil. | there is a constant need to find feed additives that improve health and nutrition of farmed fish and lessen the intestinal inflammation induced by plant-based ingredients. the objective of this study was to evaluate the effects of adding an organic acid salt to alleviate some of the detrimental effects of extreme plant-ingredient substitution of fish meal (fm) and fish oil (fo) in gilthead sea bream diet. three experiments were conducted. in a first trial (t1), the best dose (0.4%) of sodium but ... | 2016 | 27898676 |
| enhanced dietary formulation to mitigate winter thermal stress in gilthead sea bream (sparus aurata): a 2d-dige plasma proteome study. | low water temperatures during winter are common in farming of gilthead sea bream in the mediterranean. this causes metabolic disorders that in extreme cases can lead to a syndrome called "winter disease." an improved immunostimulatory nutritional status might mitigate the effects of this thermal metabolic stress. a trial was set up to assess the effects of two different diets on gilthead sea bream physiology and nutritional state through plasma proteome and metabolites. four groups of 25 adult g ... | 2017 | 27882445 |
| molecular details on gilthead sea bream (sparus aurata) sensitivity to low water temperatures from (1)h nmr metabolomics. | biometric and metabolic responses of gilthead sea bream to cold challenge are described following a growth trial divided into three water temperature steps, namely cooling, cold maintenance and recovery. experimental data provide a useful description of fish response to thermal stress at both zootechnical and molecular level. although no mortality has been observed, nuclear magnetic resonance-based metabolomics confirms the marked sensitivity of this fish species to low water temperature, and ex ... | 2017 | 27872009 |
| histochemical localization of zinc in the retina cells of gilthead sea bream (sparus aurata) fed different presentations of zinc. | to describe the distribution of zinc in the retina of a representative marine fish species and to determine whether the intracellular deposition amount correlates with the presentation of the zinc included in the practical diets (organic, inorganic, encapsulated, fish meal, and control diet), we examined the precise localization of endogenous zinc in the gilthead sea bream (sparus aurata) retina by autometallography. as observed by light microscopy, reaction products were widely distributed thro ... | 2017 | 27862297 |
| predicting gilthead sea bream (sparus aurata) freshness by a novel combined technique of 3d imaging and sw-nir spectral analysis. | a technique that combines the spatial resolution of a 3d structured-light (sl) imaging system with the spectral analysis of a hyperspectral short-wave near infrared system was developed for freshness predictions of gilthead sea bream on the first storage days (days 0-6). this novel approach allows the hyperspectral analysis of very specific fish areas, which provides more information for freshness estimations. the sl system obtains a 3d reconstruction of fish, and an automatic method locates gil ... | 2016 | 27775556 |