| linking fearfulness and coping styles in fish. | consistent individual differences in cognitive appraisal and emotional reactivity, including fearfulness, are important personality traits in humans, non-human mammals, and birds. comparative studies on teleost fishes support the existence of coping styles and behavioral syndromes also in poikilothermic animals. the functionalist approach to emotions hold that emotions have evolved to ensure appropriate behavioral responses to dangerous or rewarding stimuli. little information is however availab ... | 2011 | 22140511 |
| streptococcus agalactiae, an emerging pathogen for cultured ya-fish, schizothorax prenanti, in china. | streptococcus agalactiae (group b streptococcus) has emerged as an important pathogen that affects humans and animals, including aquatic species. s. agalactiae infections are becoming an increasing problem in aquaculture and have been reported worldwide in a variety of fish species, especially those living in warm water. recently, a very serious infectious disease of unknown aetiology broke out in ya-fish (schizothorax prenanti) farms in sichuan province. a gram-positive, chain-forming coccus wa ... | 2011 | 22146014 |
| Fish as a possible reservoir for zoonotic Giardia duodenalis assemblages. | Giardiasis is a re-emerging infectious disease of worldwide significance caused by Giardia duodenalis. This study investigated the occurrence of zoonotic G. duodenalis assemblages in fish to explore the possible role of fish in the epidemiology of human giardiasis. For this purpose, 92 fish (Tilapia nilotica and Mugil cephalus) collected from (fish farms and Nile River) at different governorates in Egypt were examined for the presence of G. duodenalis in their feces by using enzyme linked immuno ... | 2011 | 22160281 |
| laboratory exposure of oreochromis niloticus to crude microcystins (containing microcystin-lr) extracted from egyptian locally isolated strain (microcystis aeruginosa kützing): biological and biochemical studies. | cyanobacterial blooms exert negative impacts on fisheries and water management authorities. recently, it has gained global attention, as elevated earth warming and environmental pollution are accelerating algal growth. oreochromis niloticus (o. niloticus) is a worldwide and the most commonly cultured fish in egypt. the biological interaction of the living organisms to the surrounding environment must continuously be assessed to predict future effects of the ongoing hazards on fish. the study was ... | 2011 | 22095249 |
| Vaccination of sex reversed hybrid tilapia (Oreochromis niloticus × O. aureus) with an inactivated Vibrio vulnificus vaccine. | Vibrio vulnificus causes disease in economically important aquaculture raised fish and is an opportunistic human pathogen. This study reports on the isolation of V. vulnificus from diseased hybrid tilapia (Oreochromis niloticus × O. aureus) cultured in a North American water reuse facility. Our objectives were to characterize the isolate using biochemical and molecular methods, develop a disease challenge model, and determine the ability of a formalin inactivated whole-cell vaccine to protect ag ... | 2011 | 21982852 |
| autohydrolysed tilapia nilotica fish viscera as a peptone source in bacteriocin production. | fish processing generates large amounts of solid and liquid wastes. many different by-products have been produced from fish processing wastes. studies on solubilization of bolti fish (tilapia nilotica) viscera by endogenous enzymes at different phs are described. hydrolysis reactions were conducted with freshly thawed viscera utilizing an initial temperature gradient and terminated at various time points by heat inactivation of the enzymes. various peptones obtained from hydrolysed visceral homo ... | 2011 | 22654160 |
| phenological and liver antioxidant profiles of adult nile tilapia (oreochromis niloticus) exposed to toxic live cyanobacterium (microcystis aeruginosa kützing) cells. | blue-green algae (cyanobacteria) constitute the greater part of the phytoplankton. microcystis aeruginosa is amongst the most ubiquitously distributed cyanobacterial species, and almost invariably produces cyclic heptapeptide toxins called microcystins (mcs). the present study was designed to investigate the phenological and liver antioxidant profiles of the nile tilapia oreochromis niloticus chronically exposed to toxic live m. aeruginosa cells. fish were grown in the absence and presence of m. ... | 2012 | 23413757 |
| reproduction, food dynamics and exploitation level of oreochromis niloticus (perciformes: cichlidae) from artisanal fisheries in barra bonita reservoir, brazil. | nile tilapia (oreochromis niloticus), which is exotic to south america, is the most common species caught in artisanal fisheries at the barra bonita reservoir, southeastern brazil. this species is of great socioeconomic importance for the region and keeps active a population of about 500 fishers. in the present study we assess reproduction, food dynamics and level of exploitation of o. niloticus, caught by artisanal fisheries in the barra bonita reservoir. specimens were collected monthly, from ... | 2012 | 23894941 |
| characterization of four heat-shock protein genes from nile tilapia (oreochromis niloticus) and demonstration of the inducible transcriptional activity of hsp70 promoter. | heat-shock proteins (hsps), known as stress proteins and extrinsic chaperones, play important roles in the folding, translocation, and refolding/degradation of proteins. in this study, we identified four hsps in nile tilapia (oreochromis niloticus), which display conserved hsp characteristics in their predicted amino acid sequences. further analyses on the structures, homology, and phylogenetics revealed that the four hsps belong to hsp70 family. one of them does not contain introns and is named ... | 2013 | 23912482 |
| the food of the larvivorous fish gambusia affinis (baird and girard) and oreochromis (formerly tilapia) niloticus (linnaeus) in gezira irrigation canals. | the food of both gambusia affinis and oreochromis niloticus was studied. organisms eaten by both species of fish are tabulated, together with the amounts eaten during the various months of the year. g. affinis larger than 25 mm are carnivorous and become more so with age. food selection by g. affinis depends on the availability of food items rather than choice. it showed a marked preference for mosquito larvae. o. niloticus smaller than 150 mm were markedly carnivorous, but this decreased with a ... | 2010 | 2863390 |
| amino acid requirements for growth of nile tilapia. | a series of feeding experiments was conducted in aquaria to determine the quantitative requirements of the 10 essential amino acids for growth of young nile tilapia (oreochromis niloticus). the test diets contained casein and gelatin supplemented by crystalline l-amino acids to provide an amino acid profile similar to 28% whole egg protein except for the test amino acid. each set of test diets consisted of seven isonitrogenous diets containing varying levels of the amino acid to be tested. weigh ... | 2011 | 3210083 |
| metabolism of three stereoisomers of astaxanthin in the fish, rainbow trout and tilapia. | in rainbow trout (salmo gairdneri), the dietary astaxanthin diesters were mostly absorbed and accumulated in their integuments keeping their configurations, and partially metabolized to (3r, 3'r)-zeaxanthin (major) and/or (3r, 3's)-zeaxanthin (medium) and/or (3s,3's)-zeaxanthin (minor). in tilapia (tilapia nilotica), the three stereoisomers of astaxanthin diesters were promptly metabolized to only (3s,3's)-astaxanthin, and subsequently to (3r,3'r)-zeaxanthin and/or (3r,3's)-zeaxanthin and/or (3s ... | 2013 | 3829622 |
| temperature and ph effects on the total white muscle ldh of oreochromis niloticus (pisces: cichlidae). | the total lactate dehydrogenase enzyme activity of white muscle from o. niloticus shows positive thermal modulation. there is a tendency towards conservation of binding capacity for substrate at physiologically changing ph conditions. inhibition by excess pyruvate is influenced by temperature and ph, but this phenomenon is not considered to be important in anaerobic glycolysis. | 2014 | 3956161 |
| toxicants from mangrove plants, vi. heritonin, a new piscicide from th mangrove plant heritiera littoralis. | the mangrove plant heritiera littoralis has been utilized by natives of the philippines as a fish poison. the petroleum ether extract of this plant has shown toxicity to fish (tilapia nilotica). a new piscicide, which was assigned the name heritonin [1], has been identified as one of the compounds with ichthyotoxicity. the structure was elucidated by spectroscopic methods. | 1999 | 2809613 |
| comparative sequence analysis of a multidrug-resistant plasmid from aeromonas hydrophila. | aeromonas hydrophila is a pathogenic bacterium that has been implicated in fish, animal, and human disease. recently, a multidrug resistance (mdr) plasmid, pr148, was isolated from a. hydrophila obtained from a tilapia (oreochromis niloticus) farm in thailand. pr148 is a 165,906-bp circular plasmid containing 147 coding regions showing highest similarity to pndm-1_dok1, an mdr plasmid isolated from a human pathogen. pr148 was also very similar to other inca/c plasmids isolated from humans, anima ... | 2013 | 23070174 |
| myo-inositol phosphate synthase expression in the european eel (anguilla anguilla) and nile tilapia (oreochromis niloticus): effect of seawater acclimation. | a single mips gene (isyna1/ino1) exists in eel and tilapia genomes with a single myo-d-inositol 3-phosphate synthase (mips) transcript identified in all eel tissues, although two mips spliced variants [termed mips(s) and mips(l)] are found in all tilapia tissues. the larger tilapia transcript [mips(l)] results from the inclusion of the 87-nucleotide intron between exons 5 and 6 in the genomic sequence. in most tilapia tissues, the mips(s) transcript exhibits much higher abundance (generally >10- ... | 2016 | 27252471 |
| identification and characterization of micrornas in ovary and testis of nile tilapia (oreochromis niloticus) by using solexa sequencing technology. | micrornas (mirnas) are endogenous non-coding small rnas which play important roles in the regulation of gene expression by cleaving or inhibiting the translation of target gene transcripts. thereinto, some specific mirnas show regulatory activities in gonad development via translational control. in order to further understand the role of mirna-mediated posttranscriptional regulation in nile tilapia (oreochromis niloticus) ovary and testis, two small rna libraries of nile tilapia were sequenced b ... | 2014 | 24466258 |
| neurokinin b-related peptide suppresses the expression of gnrh i, kiss2 and tac3 in the brain of mature female nile tilapia oreochromis niloticus. | neurokinin b (nkb) and neurokinin b related peptide (nkbrp) belong to tachykinin peptide family. theyact as a neurotransmitter and/or neuromodulator. mutation of nkb and/or its cognate receptor, nk3r resulted in hypogonadotropic hypogonadism in mammals, implying a strong involvement of nkb/nk3r system in controlling mammalian reproduction. teleosts possess nkbrp as well as nkb, but their roles in fish reproduction need to be clarified. in this study, nkb and nkbrp coding gene (tac3) was cloned f ... | 0 | 27294210 |
| integrating cytogenetics and genomics in comparative evolutionary studies of cichlid fish. | the availability of a large number of recently sequenced vertebrate genomes opens new avenues to integrate cytogenetics and genomics in comparative and evolutionary studies. cytogenetic mapping can offer alternative means to identify conserved synteny shared by distinct genomes and also to define genome regions that are still not fine characterized even after wide-ranging nucleotide sequence efforts. an efficient way to perform comparative cytogenetic mapping is based on bac clones mapping by fl ... | 2012 | 22958299 |
| exploration of three heterotrophic nitrifying strains from a tilapia pond for their characteristics of inorganic nitrogen use and application in aquaculture water. | three heterotrophic nitrifying bacterial strains, hlf01, hbf01 and hhf01, were isolated from a pond where genetically improved farmed tilapia (gift) (oreochromis niloticus) was intensively cultured during the annual peak breeding period. analysis of biochemical, morphological characteristics and sequences analysis of 16s rdna identified strains hlf01 and hbf01 as pseudomonas sp., and strain hhf01 as acinetobacter baumannii. further analysis of heterotrophic nitrifying medium inorganic nitrogen u ... | 2015 | 25441443 |
| development of a pcr assay based on a single-base pair substitution for the detection of aeromonas caviae by targeting the gyrb gene. | aeromonas caviae is a bacterial pathogen that causes various infectious diseases in both humans and animals. to facilitate its detection, we developed species-specific primer sets targeting polymorphisms in the gyrb gene for use in a pcr assay. the technique was able to detect 100% (29/29) of the a. caviae strains tested using either of two sets of primers (designated acf1-acr and acf3-acr), which produced 293-bp and 206-bp amplicons, respectively. another set of primers (designated acf2-acr) yi ... | 2015 | 26223267 |
| identification and virulence of aeromonas dhakensis, pseudomonas mosselii and microbacterium paraoxydans isolated from nile tilapia, oreochromis niloticus, cultivated in mexico. | to identify bacterial pathogens of diseased niie tilapia oreochromis niloticus and determine their virulence. | 2013 | 23758410 |
| aeromonas jandaei and aeromonas veronii caused disease and mortality in nile tilapia, oreochromis niloticus (l.). | diseases caused by motile aeromonads in freshwater fish have been generally assumed to be linked with mainly aeromonas hydrophila while other species were probably overlooked. here, we identified two isolates of non-a. hydrophila recovered from nile tilapia exhibiting disease and mortality after exposed to transport-induced stress and subsequently confirmed their virulence in artificial infection. the bacterial isolates were identified as aeromonas jandaei and aeromonas veronii based on phenotyp ... | 2017 | 28383126 |
| identification and characterization of c1 inhibitor in nile tilapia (oreochromis niloticus) in response to pathogenic bacteria. | c1 inhibitor (c1inh) is a multi-functional serine protease inhibitor in plasmatic cascades, not only inactivating various proteases, but also regulating both complement and contact system activation. in this study, we described the identification and characterization of a c1inh ortholog from nile tilapia (oreochromis niloticus) at molecular, protein and cellular levels. the full-length cdna of oreochromis niloticus c1inh (onc1inh) consisted of 1791 bp of nucleotide sequence encoding polypeptides ... | 2017 | 27986601 |
| macroalgal activity against multiple drug resistant aeromonas hydrophila: a novel treatment study towards enhancement of fish growth performance. | the aim of this study was to evaluate the efficiency of macroalgal extracts as antibacterial agent against multidrug-resistant (mdr) bacteria isolated from nile tilapia (oreochromis niloticus) as well as to enhance the fish growth performance by macroalgae diet application. | 2016 | 27840224 |
| cytotoxic effects of aeromonas hydrophila culture supernatant on peripheral blood leukocytes of nile tilapia (oreochromis niloticus): possible presence of a secreted cytotoxic lectin. | number of exotoxins like haemolysin, leukocidin, aerolysin etc. were reported from aeromonas hydrophila. in this study, we report the haemolytic and cytotoxic effect of a. hydrophila culture supernatant (cs) that is specifically inhibited by lactose and also by serum and mucus of nile tilapia (oreochromis niloticus). hence, we assume the presence of a secreted lectin in the cs. cs is toxic to peripheral blood leukocytes (pbl) of o. niloticus as revealed by mtt assay and by flow cytometry. dna la ... | 2016 | 27702674 |
| dietary supplementation with xylanase-expressing b. amyloliquefaciens r8 improves growth performance and enhances immunity against aeromonas hydrophila in nile tilapia (oreochromis niloticus). | bacillus amyloliquefaciens has attracted attention as a probiotic in aquaculture due to its immunostimulatory activity against pathogenic infection. xylanases are extensively used in animal feed to degrade plant ingredients, enhancing nutrient utilization and increasing the growth rate of various animals. in the present study, the effects of dietary supplementation with b. amyloliquefaciens and xylanase-expressing b. amyloliquefaciens r8 on the growth of nile tilapia (oreochromis niloticus) and ... | 2016 | 27670086 |
| dietary camu camu, myrciaria dubia, enhances immunological response in nile tilapia. | camu camu, myrciaria dubia, is an amazon plant that presents high levels of vitamin c in its composition. several studies in animals and humans have demonstrated their efficiency in the prevention and treatment of various diseases. however, there are no reports of its properties in fish. the aim of this study was to evaluate the effect of the oral administration of the extract of this plant in the immune parameters in nile tilapia, oreochromis niloticus. 400 nile tilapia (80 ± 5 g) were randomly ... | 2016 | 27539707 |
| ceftiofur pharmacokinetics in nile tilapia oreochromis niloticus after intracardiac and intramuscular administrations. | ceftiofur is a broad-spectrum third generation cephalosporin, which acts by inhibiting bacterial cell wall synthesis. it is active against gram-positive and gram-negative bacteria such as aeromonas hydrophila and β-lactamase-producing strains, which are common pathogens in freshwater fish. ceftiofur pharmacokinetics in nile tilapia oreochromis niloticus were studied following single intracardiac (i.c.) or intramuscular (i.m.) administration of ceftiofur sodium (naxcel®) in a dose of 5 mg ceftiof ... | 2016 | 27596857 |
| effects of a novel marine probiotic, lactobacillus plantarum ah 78, on growth performance and immune response of nile tilapia (oreochromis niloticus). | evaluation of the efficacy of marine lactic acid bacteria, lactobacillus plantarum ah 78, on growth performance and immune response of nile tilapia (oreochromis niloticus) challenged with aeromonas hydrophila. | 2016 | 26834088 |
| eubiotic effect of a dietary acidifier (potassium diformate) on the health status of cultured oreochromis niloticus. | in connection with the global demand for safe human food and the production of environmentally friendly aquaculture products, acidifiers are natural organic acids and salts that have received considerable attention as animal-feed additives. the current study was designed to evaluate the effects of potassium diformate (kdf) on the growth performance and immunity of cultured oreochromis niloticus (o. niloticus). four iso-nitrogenous and iso-caloric rations containing graded levels of kdf, includin ... | 2015 | 26199753 |
| skin and subcutaneous mycoses in tilapia (oreochromis niloticus) caused by fusarium oxysporum in coinfection with aeromonas hydrophila. | subcutaneous mycoses in freshwater fish are rare infections usually caused by oomycetes of the genus saprolegnia and some filamentous fungi. to date, fusarium infections in farmed fish have only been described in marine fish. here, we report the presence of fusarium oxysporum in subcutaneous lesions of nile tilapia (oreochromis niloticus). histopathologic evaluation revealed granuloma formation with fungal structures, and the identity of the etiological agent was demonstrated by morphological an ... | 2015 | 26155462 |
| effects of replacing soybean meal with rubber seed meal on growth, antioxidant capacity, non-specific immune response, and resistance to aeromonas hydrophila in tilapia (oreochromis niloticus × o. aureus). | this study evaluated the effects of replacing soybean meal (sbm) with rubber seed meal (rsm) on growth, antioxidant capacity, non-specific immune response and resistance to aeromonas hydrophila in tilapia (oreochromis niloticus × oreochromis aureus). five experimental diets were formulated with 0 (control), 10, 20, 30, and 40% rsm replacing graded levels of sbm, respectively. fish were fed one of the five experimental diets for eight weeks, and then challenged by a. hydrophila via intraperitonea ... | 2015 | 25804486 |
| quantitative and qualitative analyses of the bacterial microbiota of tilapia (oreochromis niloticus) cultured in earthen ponds in the philippines. | the quantity and composition of the bacterial microbiota in the rearing water, sediment, gills and intestines of tilapia oreochromis niloticus collected every 2 weeks from day 30 to day 120 after stocking for grow-out culture in 6 earthen brackish water ponds in the philippines were examined. the total heterotrophic aerobic bacterial counts obtained in the water, sediment, gills and intestines of tilapia ranged from 10(3) to 10(4) c.f.u. ml(-1), 10(3)-10(5), 10(5)-10(7) and 10(4)-10(7) c.f.u. g( ... | 2015 | 25555375 |
| dietary supplementation with bacillus subtilis, saccharomyces cerevisiae and aspergillus oryzae enhance immunity and disease resistance against aeromonas hydrophila and streptococcus iniae infection in juvenile tilapia oreochromis niloticus. | a feeding trial was conducted to investigate the effects of dietary administration of probiotic with bacillus subtilis, aspergillus oryzae and saccharomyces cerevisiae on growth, innate immune response, hemato-immunological parameters and disease resistance of nile tilapia, oreochromis niloticus. animals were distributed in three equal groups, each of five replicates and received one of the following experimental diets for four weeks: control, non-supplemented diet; 5 g kg(-1) probiotic mixture ... | 2015 | 25530581 |
| effects of chito-oligosaccharides supplementation on growth performance, intestinal cytokine expression, autochthonous gut bacteria and disease resistance in hybrid tilapia oreochromis niloticus ♀ × oreochromis aureus ♂. | we investigated the effects of incorporating chitinase (chib565)-hydrolyzed shrimp shell chitin into the diet of hybrid tilapia (oreochromis niloticus ♀ × oreochromis aureus ♂) with regard to production, intestinal immune status and autochthonous gut bacteria, and protection against bacterial pathogen aeromonas hydrophila. five experimental diets were formulated by supplementing the basal diet with the hydrolyzed shrimp shell chitin (0.0%, t1 control; 0.8%, t3; 1.6%, t4; or 2.4%, t5) or 0.1% com ... | 2014 | 25038280 |
| immunostimulatory effects of artificial feed supplemented with a chinese herbal mixture on oreochromis niloticus against aeromonas hydrophila. | the effects of a chinese herbal mixture (chm) composed of astragalus, angelica, hawthorn, licorice root and honeysuckle on immune responses and disease resistant of nile tilapia (oreochromis niloticus gift strain) were investigated in present study. fish were fed diets containing 0 (control), 0.5%, 1.0%, 1.5% or 2.0% chm (w/w) for 4 weeks. and series of immune parameters including lysozyme, cytokine genes tnf-α and il-1β, superoxide dismutase (sod), peroxidase (pod), malondialdehyde (mda) were m ... | 2014 | 24925761 |
| the effect of mistletoe, viscum album coloratum, extract on innate immune response of nile tilapia (oreochromis niloticus). | the purpose of the present study was to evaluate the effect of dietary mistletoe extracts on non-specific immune response and disease resistance of nile tilapia (oreochromis niloticus) against aeromonas hydrophila infection. tilapia fingerlings were fed with a diet containing 0 mg as a control, 10 mg, 50 mg, and 200 mg mistletoe powder kg(-1) dry diet for 80 days. the immunological parameters, respiratory burst activity, lysozyme activity, alternative complement haemolysis activity (ach(50)), an ... | 2012 | 22554573 |
| application of fungal chitosan incorporated with pomegranate peel extract as edible coating for microbiological, chemical and sensorial quality enhancement of nile tilapia fillets. | fish are generous sources for providing man with his essential nutritional requirements, but the extreme susceptibility to quality deterioration hinders their optimal usage and storage. natural derivatives are always the perfect alternatives for food preservation. the application of fungal chitosan (ch), from aspergillus niger, and pomegranate peel extract (ppe), in coating films for nile tilapia (oreochromis niloticus) fillets preservation and maintaining their microbiological, chemical and se ... | 2017 | 28274866 |
| improvement of immunity and disease resistance in the nile tilapia, oreochromis niloticus, by dietary supplementation with bacillus amyloliquefaciens. | probiotics can be used as immunostimulants in aquaculture. the aim of this study was to evaluate the immune responses of nile tilapia oreochromis niloticus following feeding with bacillus amyloliquefaciens spores at concentrations of 1 × 10(6) (g3) and 1 × 10(4) (g2) colony-forming units per gram (cfu/g) of feed compared with a basal diet with no probiotics (g1). a total of 180 fingerlings (27.7 ± 0.22 g) were divided into three groups (g1-g3 of 20 fish per group) in triplicate. innate immunitie ... | 2015 | 25783002 |
| molecular characterization and biological effects of a c-type lectin-like receptor in large yellow croaker (larimichthys crocea). | the c-type lectin-like receptors (ctlrs) play important roles in innate immunity as one type of pattern recognition receptors. here, we cloned and characterized a c-type lectin-like receptor (lycctlr) from large yellow croaker larimichthys crocea. the full-length cdna of lycctlr is 880 nucleotides long, encoding a protein of 215 amino acids. the deduced lycctlr contains a c-terminal c-type lectin-like domain (ctld), an n-terminal cytoplasmic tail, and a transmembrane region. the ctld of lycctlr ... | 2015 | 26690423 |
| genotoxic evaluation in oreochromis niloticus (fish: characidae) of recombinant spore-crystal complexes cry1ia, cry10aa and cry1ba6 from bacillus thuringiensis. | bioinsecticides from bacillus thuringiensis (bt) are widely used around the world in biological control against larval stages of many insect species. bt has been considered a biopesticide that is highly specific to different orders of insects, non-polluting and harmless to humans and other vertebrates, thus becoming a viable alternative for combating agricultural pests and insect vectors of diseases. the family of bt δ-endotoxins are crystal-protein inclusions showing toxicity to insects' midgut ... | 2014 | 24374720 |
| infectious bacterial pathogens, parasites and pathological correlations of sewage pollution as an important threat to farmed fishes in egypt. | this paper is a part of a multi-disciplinary research "application of decentralized on-site water treatment system in egypt for use in agriculture and producing safe fish and animal proteins". the project aimed to investigate the environmental impact of implementing sewage water before and after treatment using the effluent of the on-site decentralized japanese' johkasou system, in agriculture and producing fish protein. the aim is to establish such system in egypt to strengthen the sanitary con ... | 2016 | 27720545 |
| metacercarial infection of wild nile tilapia (oreochromis niloticus) from brazil. | fingerlings of oreochromis niloticus collected in an artificial urban lake from belo horizonte, minas gerais, brazil, were evaluated for natural infection with trematodes. morphological taxonomic identification of four fluke species was performed in o. niloticus examined, and the total prevalence of metacercariae was 60.7% (37/61). centrocestus formosanus, a heterophyid found in the gills, was the species with the highest prevalence and mean intensity of infection (31.1% and 3.42 (1-42), resp.), ... | 2014 | 25485302 |
| molecular characterization and expression analysis of toll-like receptor 2 in response to bacteria in silvery pomfret intestinal epithelial cells. | toll-like receptor 2 (tlr2) has been shown to play a crucial role in the host defense of pathogenic microbes in innate immunity. in this study, the full-length cdna of tlr2 in silvery pomfret (pampus argenteus) was cloned by homology cloning and the rapid amplification of cdna ends (race) technique. the complete cdna sequence of tlr2 was 2932 bp, containing an open reading frame (orf) of 2469 bp encoding 822 amino acids. a multiple alignment analysis of the silvery pomfret tlr2 protein-coding se ... | 2016 | 27574826 |
| possible role of fish and frogs as paratenic hosts of dracunculus medinensis, chad. | copepods infected with dracunculus medinensis larvae collected from infected dogs in chad were fed to 2 species of fish and tadpoles. although they readily ingested copepods, neither species of fish, nile tilapia (oreochromis niloticus) nor fathead minnow (pimephalis promelas), were found to harbor dracunculus larvae when examined 2-3 weeks later. tadpoles ingested copepods much more slowly; however, upon examination at the same time interval, tadpoles of green frogs (lithobates [rana] clamitans ... | 2016 | 27434418 |
| microbial chitosan as a biopreservative for fish sausages. | processed fish products are worthy sources to supply man with his main nutritional needs, but they are extremely susceptible to quality loss during storage. microbial (fungal) chitosan is a bioactive polymer that has numerous applications in health promoting fields. fungal chitosan was extracted from the grown mycelia of aspergillus brasiliensis (niger) to investigate its potential role as antimicrobial, preservative and quality improvement agent, in processed fish sausages from nile tilapia (or ... | 2016 | 27565293 |
| molecular structure, distribution, and immunology function of tnfsf13b (baff) in nile tilapia (oreochromis niloticus). | b cell-activating factor (baff)is a member of the tumor necrosis factor (tnf) family and plays roles in b cell survival and maturation. in this study, the full-length cdna of nile tilapia (oreochromis niloticus) baff (tbaff) was amplified from the spleen by reverse transcription pcr (rt-pcr). the open reading frame of this cdna encodes a protein of 261 amino acids containing a predicted transmembrane domain and a furin protease cleavage site, similar to mammalian, avian, and reptile baff. real-t ... | 2016 | 26915306 |
| dietary administration of bacillus subtilis on hematology and non-specific immunity of nile tilapia oreochromis niloticus raised at different stocking densities. | an 84-day feeding trial was conducted to evaluate the effect of the dietary administration of bacillus subtilis on the growth performance, body composition, intestinal probiotic recovery, hematology, and non-specific immunity of nile tilapia (oreochromis niloticus) raised at two stocking densities. five hundred twenty male nile tilapias (32.63 ± 1.25 g) were distributed in 16,800-l tanks. the experimental design was completely randomized using four replications and a 2 × 2 factorial scheme with ... | 2014 | 24878743 |
| modulation of the intestinal microbiota and morphology of tilapia, oreochromis niloticus, following the application of a multi-species probiotic. | the intestinal microbiota and morphology of tilapia (oreochromis niloticus) were investigated after the application of a multi-species probiotic containing lactobacillus reuteri, bacillus subtilis, enterococcus faecium and pediococcus acidilactici (aquastar(®) growout). tilapia (55.03 ± 0.44 g) were fed either a control diet or a probiotic diet (control diet supplemented with aquastar(®) growout at 5 g kg(-1)). after four and eight weeks, culture-dependent analysis showed higher levels of lactic ... | 2015 | 26115752 |
| dietary administration of bacillus subtilis hainup40 enhances growth, digestive enzyme activities, innate immune responses and disease resistance of tilapia, oreochromis niloticus. | the probiotic properties of bacillus subtilis hainup40 isolated from the aquatic environment, and the effects of dietary administration of b. subtilis hainup40 on the growth performance, intestinal probiotic recovery, digestive enzyme activities, innate immunity and disease resistance of tilapia (oreochromis niloticus) were evaluated. the probiotic properties investigated include tolerance to simulated gastrointestinal stress, auto-aggregation, cell surface hydrophobicity and extracellular enzym ... | 2017 | 27919757 |
| isolation and characterization of bacteria with antibacterial properties from nile tilapia (oreochromis niloticus). | one hundred and twenty bacterial isolates were obtained from the intestinal mucus of nile tilapia (oreochromis niloticus) and screened for antagonistic activity and adherence abilities. based on in vitro antagonism against two pathogens (streptococcus iniae and edwardsiella piscicida), five isolates were selected and identified by 16s rrna gene sequence analysis. all antagonistic isolates were affiliated to the genus bacillus, which showed inhibitory activity against s. iniae. only the isolate b ... | 2016 | 27033910 |
| effects of dietary bacillus licheniformis on growth performance, immunological parameters, intestinal morphology and resistance of juvenile nile tilapia (oreochromis niloticus) to challenge infections. | the effects of oral administration of bacillus licheniformis on growth performance, immunity, intestinal morphology and disease resistance of juvenile tilapia were investigated. six experimental diets supplemented with different concentrations of b. licheniformis (0%, 0.02%, 0.04%, 0.06%, 0.08% and 0.1% of alcare(®), containing live germ 2 × 10(10) cfu/g) were formulated, viz. control, t1, t2, t3, t4 and t5. each diet was randomly assigned to triplicate groups of 30 fishes (3.83 ± 0.03 g). after ... | 2015 | 26108035 |
| antimicrobial susceptibility of escherichia coli isolated from fresh-marketed nile tilapia (oreochromis niloticus). | the contamination of seafood by bacteria of fecal origin, especially escherichia coli, is a widely documented sanitary problem. the objective of the present study was to isolate e. coli strains from the gills, muscle, and body surface of farmed nile tilapias (oreochromis niloticus) fresh-marketed in supermarkets in fortaleza (ceará, brazil), to determine their susceptibility to antibiotics of different families (amikacin, gentamicin, imipenem, cephalothin, cefotaxime, ciprofloxacin, aztreonam, a ... | 2014 | 24808957 |
| the influence of fish culture in floating net cages on microbial indicators of water quality. | this work was carried out to analyse the microbiological parameters of the water quality of a reservoir used for the irrigation and culture of tilapia (oreochromis niloticus) in floating net cages. the physico-chemical parameters, counts of mesophilic total aerobic bacteria, total and thermotolerant coliforms and fecal streptococci, and the presence of escherichia coli in samples of water collected in three sites of the reservoir (pre-culture site, culture site, post-culture site) were analysed. ... | 2013 | 24212684 |
| effects of dietary acidifiers on growth, hematology, immune response and disease resistance of nile tilapia, oreochromis niloticus. | organic acids or their salts can be used as feed additives in aquaculture. this study was conducted to evaluate the use of a mixture of formic acid, propionic acid and calcium propionate compared with oxytetracycline (otc). a total of 720 apparently healthy oreochromis niloticus fingerlings with an average body weight of 28.8 ± 0.14 g (mean ± se) were randomly divided into four equal groups (g1, g2, g3and g4). g1 was fed a basal diet with no additive as the control group, while g2 and g3 were fe ... | 2016 | 26860238 |
| molecular characterization and increased expression of the nile tilapia, oreochromis niloticus (l.), t-cell receptor beta chain in response to streptococcus agalactiae infection. | the complete cdna sequence of the nile tilapia t-cell receptor (tcr) β chain was cloned using 5' race. the full-length, 1263-bp cdna contained a 942-bp open reading frame (orf) encoding a 314-amino-acid protein. sequence analyses revealed that the nile tilapia tcr β chain contains four conserved cysteine residues involved in the formation of disulphide bridges and a conserved amino acid motif believed to be important for assembly and signalling of the tcr αβ/cd3 complex, both of which are normal ... | 2012 | 22417380 |
| lactobacillus planarum subsp. plantarum jcm 1149 vs. aeromonas hydrophila nj-1 in the anterior intestine and posterior intestine of hybrid tilapia oreochromis niloticus ♀ × oreochromis aureus ♂: an ex vivo study. | to investigate the ex vivo interactions of probiotic-pathogen-host in warm-water fish, hybrid tilapia (oreochromis niloticus♀ × oreochromis aureus♂) were sacrificed to isolate anterior and posterior intestine for incubation with phosphate-buffered saline (pbs; ph 7.2) as the control, lactobacillus plantarum jcm 1149 at 1.0 × 10(9) cfu/ml, aeromonas hydrophila nj-1 at 1.0 × 10(8) cfu/ml, or the both combination. denaturing gradient gel electrophoresis (dgge) fingerprint and consequent sequence an ... | 2013 | 23664914 |
| honey bee pollen improves growth, immunity and protection of nile tilapia (oreochromis niloticus) against infection with aeromonas hydrophila. | the mode of action of honey bee pollen (hbp) was investigated in nile tilapia (oreochromis niloticus) challenged with aeromonas hydrophila. thus, fish with an average weight of 29 ± 3 g were divided into four groups, and fed with hbp-free diet (control), and 1%, 2.5% and 4% (w/v) hbp incorporated into basal diet for 10, 20 and 30 days. immunological, hematological, biochemical and growth parameters were measured, and sub-groups of fish were challenged with a. hydrophila via intraperitoneal injec ... | 2014 | 25086230 |
| effects of partially replacing dietary soybean meal or cottonseed meal with completely hydrolyzed feather meal (defatted rice bran as the carrier) on production, cytokines, adhesive gut bacteria, and disease resistance in hybrid tilapia (oreochromis niloticus ♀ × oreochromis aureus ♂). | we formulated experimental diets for hybrid tilapia to investigate the effects of replacing dietary soybean meal (sbm) or cottonseed meal (csm) by completely hydrolyzed feather meal (defatted rice bran as the carrier; abbreviated as chfm), with emphasis on fish growth, the composition of adhesive gut bacteria, intestinal and hepatic immune responses, and disease resistance. a series of four isonitrogenous (33% crude protein) and isolipidic (6% crude lipid) diets were formulated to replace the is ... | 2014 | 25304546 |
| shiga toxigenic and enteropathogenic escherichia coli in water and fish from pay-to-fish ponds. | escherichia coli is part of the normal microflora of the intestines of mammals. however, among the enteric pathogens, it is one of the leading causes of intestinal diseases, especially shiga toxigenic e. coli, which can cause diarrhoea, haemorrhagic colitis and complications like haemolytic uraemic syndrome and thrombotic thrombocytopaenic purpura. escherichia coli is considered a serious public health problem. water and fish samples were subjected to biochemical tests to confirm the presence of ... | 2016 | 26671650 |
| synergistic effects of micro-electrolysis-photocatalysis on water treatment and fish performance in saline recirculating aquaculture system. | a new physico-chemical process for tan (total ammonia nitrogen) removal and disinfection is introduced in saline recirculating aquaculture system (ras), in which the biofilter is replaced with an integrated electrolysis cell and an activated carbon filter. the electrolysis cell which is based on micro current electrolysis combined with uv-light was self-designed. after the fundamental research, a small pilot scale ras was operated for 30 days to verify the technical feasibility. the system was s ... | 2017 | 28345583 |
| glucose enhances tilapia against edwardsiella tarda infection through metabolome reprogramming. | we have recently reported that the survival of tilapia, oreochromis niloticus, during edwardsiella tarda infection is tightly associated with their metabolome, where the survived o. niloticus has distinct metabolomic profile to dying o. niloticus. glucose is the key metabolite to distinguish the survival- and dying-metabolome. more importantly, exogenous administration of glucose to the fish greatly enhances their survival for the infection, indicating the functional roles of glucose in metabolo ... | 2017 | 27965164 |
| hematology and productive performance of nile tilapia (oreochromis niloticus) naturally infected with flavobacterium columnare. | columnaris disease is one of the main causes of mortality in tilapia rearing and is responsible for large economic losses worldwide. hematology is a tool that makes it possible to study organisms' physiological responses to pathogens. it may assist in making diagnoses and prognoses on diseases in fish populations. the hematological variables of nile tilapia were studied in specimens with a clinical diagnosis of columnaris disease and in specimens that were disease-free. the total erythrocyte cou ... | 2011 | 24031633 |
| draft genome sequence of francisella noatunensis subsp. orientalis stir-gus-f2f7, a highly virulent strain recovered from diseased red nile tilapia farmed in europe. | a highly virulent strain of francisella noatunensis subsp. orientalis, stir-gus-f2f7, was isolated from moribund red nile tilapia (oreochromis niloticus) farmed in europe. in this communication, the complete genome sequencing of this bacterium is reported. | 2017 | 28302784 |
| edwardsiella ictaluri as the causative agent of mortality in cultured nile tilapia. | edwardsiella ictaluri was consistently isolated from the spleens, livers, and head kidneys of diseased nile tilapia oreochromis niloticus from a farm experiencing mortality events in several culture ponds. we describe the first published outbreak of e. ictaluri-induced edwardsiellosis in nile tilapia. pure cultures of the isolated bacteria were characterized both biochemically and molecularly. biochemical analysis was performed using the api-20e and rapid one systems, and antimicrobial susceptib ... | 2012 | 22838078 |
| bacterial distribution and tissue targets following experimental edwardsiella ictaluri infection in nile tilapia oreochromis niloticus. | edwardsiella ictaluri, a gram-negative enteric bacterium, is the known etiological agent of enteric septicemia of catfish. in the last few years, different strains have been implicated as the causative agent of mortality events in cultured fish, including nile tilapia oreochromis niloticus l. due to the emergent nature of edwardsiellosis in non-ictalurid fish, little is known about the dynamics of e. ictaluri infection in tilapia. the purpose of this study was to gain a better understanding of t ... | 2013 | 23709463 |
| development of a bacterial challenge test for gnotobiotic nile tilapia oreochromis niloticus larvae. | gastrointestinal microbiota have an important impact on fish health and disease, stimulating interest in a better understanding of how these gastrointestinal microbial communities are composed and consequently affect host fitness. in this respect, probiotic microorganisms have been extensively used in recent aquaculture production. to study the use of probiotics in the treatment of infectious diseases, the establishment of a method of experimental infection to obtain consistent results for morta ... | 2014 | 24781794 |
| comparison of edwardsiella ictaluri isolates from different hosts and geographic origins. | the intraspecific variability of e. ictaluri isolates from different origins was investigated. isolates were recovered from farm-raised catfish (ictalurus punctatus) in mississippi, usa, tilapia (oreochromis niloticus) cultured in the western hemisphere and zebrafish (danio rerio) propagated in florida, usa. these isolates were phenotypically homologous and antimicrobial profiles were largely similar. genetically, isolates possessed differences that could be exploited by repetitive-sequence-medi ... | 2016 | 26661707 |
| effect of poly-β-hydroxybutyrate on growth and disease resistance of nile tilapia oreochromis niloticus juveniles. | the growth promoting effect of the bacterial storage compound poly-β-hydroxybutyrate (phb) has been studied for young fish of high trophic level (european sea bass) and intermediate trophic level (siberian sturgeon). here, the effect of phb on growth, digestive enzyme activities, body composition and diseases resistance of juvenile nile tilapia (oreochromis niloticus) of low trophic level was investigated. although dietary phb supplementation (5, 25 and 50 g phb kg(-1) formulated semi-purified d ... | 2016 | 26711027 |
| infection dynamics of cichlidogyrus tilapiae and c. sclerosus (monogenea, ancyrocephalinae) in nile tilapia (oreochromis niloticus l.) from uganda. | the infection dynamics of the gill monogeneans cichlidogyrus tilapiae and c. sclerosus on oreochromis niloticus with respect to habitat type (reservoir, stream, ponds and cages), host sex, size and seasons was determined between january and november 2008. during the study period, 45.2% of the 650 fish examined were infected with cichlidogyrus spp. the infected hosts harboured an average of 8.6 ± 3.4 parasites/fish. across habitat types, the proportion of infected fish was not statistically diffe ... | 2012 | 21791155 |
| parasites of native cichlidae populations and invasive oreochromis niloticus (linnaeus, 1758) in tributary of amazonas river (brazil). | this study provides the first investigation on acquisition of parasites in invasive o. niloticus by parasite species of native cichlidae from the igarapé fortaleza basin, northern brazil. there were examined 576 specimens of 16 species of native cichlids and invasive o. niloticus collected in the main channel and the floodplain area of this tributary of amazon river. the invasive o. niloticus was poorly parasitized having only ichthyophthirius multifiliis, trichodina centrostrigeata, paratrichod ... | 2014 | 24728360 |
| fish-borne trematodes in cultured nile tilapia (oreochromis niloticus) and wild-caught fish from thailand. | fish-borne zoonotic trematode (fzt) infections affect the health of more than 18 million people around the world, particularly in asian countries. nile tilapia (oreochromis niloticus) is a white meat fish that has an increasing national and international market. the objective of this study was to determine the prevalence of fzt metacercarial infections in nile tilapia from cage and pond aquaculture systems and in wild-caught fish from suphan buri, nakhon pathom and chachoengsao provinces, thaila ... | 2013 | 24029716 |
| redescription of cichlidogyrus tiberianus paperna, 1960 and c. dossoui douëllou, 1993 (monogenea: ancyrocephalidae), with special reference to the male copulatory organ. | the flatworms of the genus cichlidogyrus paperna, 1960 (monogenea: ancyrocephalidae) are gill parasites of freshwater fish, affecting predominantly the family cichlidae. cichlidogyrus tiberianus paperna, 1960 and cichlidogyrus dossoui douëllou, 1993 are among the most widely distributed species of the genus, occurring in several african river basins and infecting many different host species, including the economically important nile tilapia oreochromis niloticus (linnaeus) and redbreast tilapia ... | 2017 | 28062988 |
| monogenean community structure of oreochromis niloticus in relation to heavy metal pollution and host reproductive cycle. | the community structure of monogenean parasites of oreochromis niloticus and its relation to the variations of heavy metal concentrations in water and the host reproductive cycle (gonadosomatic index, gsi) was studied. fish were collected from fishermen lake, ismailia. monogenea community consisted of seven species, cichlidogyrus halli typicus (57.8%), c. thurstonae (35.3%), c. ergensi (13.8 %), c. tiberianus (16.4%), c. arthracanthus (13.8%), scutogyrus longicornis (22.4%) and gyrodadtylus cicc ... | 2012 | 22662591 |
| ectoparasites of nile tilapia (oreochromis niloticus) in cage farming in a hydroelectric reservoir in brazil. | for this study, we performed a parasitological analysis of cage-cultured nile tilapia (oreochromis niloticus) from the água vermelha reservoir, southeastern brazil, and verified relationships with limnological data, seasonality, and fish growth phase. from march 2010 to march 2011, sixty-three specimens of o. niloticus in three growth phases (i.e., initial, intermediate, and final) were collected. all fish specimens were infested with at least one ectoparasite species (prevalence = 100%). five s ... | 2014 | 25054495 |
| gill monogeneans of oreochromis niloticus (linnaeus, 1758) and oreochromis leucostictus (trewavas, 1933) in lake naivasha, kenya. | an investigation of gill monogeneans from the nile tilapia oreochromis niloticus and the blue spotted tilapia oreochromis leucostictus (50 individuals per species) was done between the months of november 2014 to february 2015 in lake naivasha, kenya. standard parasitological procedures were used to examine fish gills for the presence of monogeneans. the observed monogeneans were collected, preliminarily identified using identification keys, quantified and fixed in 4 % formalin for morphological ... | 2016 | 26691859 |
| field assessment of the mid winter mass kills of trophic fishes at mariotteya stream, egypt: chemical and biological pollution synergistic model. | pathogenic candida albicans was isolated from water and fish samples collected during an emergent event of mass mortalities among the juvenile nile tilapia (oreochromis niloticus), sharp toothed catfish (clarias gariepinus) along the stream of mariotteya drainage. investigations indicated that fish mortalities were confined to the area of shubramant and aboul noumros (north to sakara 7 drainage). c. albicans was isolated from the lesions associated with multiple skin ulcers in both nile tilapia ... | 2013 | 23075545 |
| microbiological and chemical safety concerns regarding frozen fillets obtained from pangasius sutchi and nile tilapia exported to european countries. | microbiological and chemical safety concerns regarding frozen fillets from pangasius catfish (pangasius hypophthalmus) exported to poland, germany and ukraine and nile tilapia (oreochromis niloticus) exported to poland and germany were investigated by analyzing heavy metal residues, microbiological hazards, biogenic amines, and thiobarbituric acid (tba) and total volatile basic nitrogen (tvb-n) content. | 2016 | 25907121 |
| phosphoglycerate kinase enhanced immunity of the whole cell of streptococcus agalactiae in tilapia, oreochromis niloticus. | streptococcus agalactiae is a gram-positive bacterium and a severe aquaculture pathogen that can infect a wide range of warmwater fish species. the outer-surface proteins in bacterial pathogens play an important role in pathogenesis. we evaluated the immunogenicity of two of the identified surface proteins namely phosphoglycerate kinase (pgk) and ornithine carbamoyl-transferase (oct). pgk and oct were over-expressed and purified from escherichia coli and used as the subunit vaccines in tilapia. ... | 2014 | 25218275 |
| production, gene structure and characterization of two orthologs of leptin and a leptin receptor in tilapia. | full-length cdna encoding two leptin sequences (tlepa and tlepb) and one leptin receptor sequence (tlepr) were identified in tilapia (oreochromis niloticus). the full-length cdna of tlepr was 3423bp, encoding a protein of 1140 amino acid (aa) which contained all functionally important domains conserved among vertebrate leptin receptors. the cdnas of tlepa and tlepb were 486bp and 459bp in length, encoding proteins of 161 aa and 152 aa, respectively. modeling the three-dimensional structures of t ... | 2014 | 24852346 |
| [identification and pathological observation of a pathogenic plesiomonas shigelloides strain isolated from cultured tilapia (oreochromis niloticus)]. | a mass mortality of tilapia broke out in an aquaculture farm in panyu, guangdong province in may, 2013. affected fish showed blackening of body color, haemorrhageing on surface, scales shedding, fin rotting, and the presence of yellow liver, dark red spleen, enlarged gallbladder and ascitic fluid in the abdominal cavity. the purpose of this research was isolating and identifying the pathogen. | 2015 | 25958688 |
| occurrence of plesiomonas shigelloides in cultured red hybrid tilapia (oreochromis niloticus) from tropical rivers, east coast malaysia. | a total of 182 isolates of plesiomonas shigelloides were identified from 40 healthy red hybrid tilapia, oreochromis niloticus cultured at two important rivers in terengganu, malaysia namely como river and terengganu river from east coast malaysia. p. shigelloides count in digestive tract content (dtc) and muscle (mus) of red hybrid tilapia cultured at terengganu river was 1000-fold higher than como river. antibiotic susceptibility test was also performed on plesiomonas shigelloides isolates. the ... | 2012 | 24191623 |
| eutrophication, ammonia intoxication, and infectious diseases: interdisciplinary factors of mass mortalities in cultured nile tilapia. | the present study was designed to assess the possible causes of the mass mortalities of nile tilapia oreochromis niloticus at el-behera governorate, egypt, in relationship to environmental and microbiotic factors. water samples were collected from fish farms at different locations and from lake edku to analyze water temperature, water ph, salinity, biological oxygen demand, dissolved oxygen, total ammonia nitrogen, and un-ionized ammonia. a number of moribund and freshly dead fish were sampled a ... | 2016 | 27484819 |
| molecular cloning and expression analysis of small ubiquitin-like modifier (sumo) genes from grouper (epinephelus coioides). | small ubiquitin-like modifier (sumo) is a group of proteins binding to lysine residues of target proteins and thereby modifying their stability, activity and subcellular localization. in the present study, two sumo homolog genes (ecsumo1 and ecsumo2) from grouper (epinephelus coioides) were cloned and characterized. the full-length sequence of ecsumo1 was 749 bp in length and contained a predicted open reading frame of 306 bp encoding 101 amino acids with a molecular mass of 11.34 kda. the full- ... | 2016 | 26616235 |
| genetic characterization of a betanodavirus isolated from a clinical disease outbreak in farm-raised tilapia oreochromis niloticus (l.) in thailand. | betanodavirus infection was diagnosed in larvae of farm-raised tilapia oreochromis niloticus (l.), in central thailand. extensive vacuolar degeneration and neuronal necrosis were observed in histological sections with positive immunohistochemical staining for betanodavirus. molecular phylogenetic analysis was performed based on the nucleotide sequences (1333 bases) of the capsid protein gene. the virus strain was highly homologous (93.07-93.88%) and closely related to red-spotted grouper nervous ... | 2015 | 24164433 |
| lack of association between flavobacterium columnare genomovar and virulence in hybrid tilapia oreochromis niloticus (l.)×oreochromis aureus (steindachner). | columnaris disease can be problematic in tilapia (oreochromis spp.) production. an understanding of the pathogenesis and virulence of flavobacterium columnare is needed to develop prevention strategies. the objective of this study was to determine the virulence of genetically defined isolates of f. columnare in sex-reversed hybrid tilapia, oreochromis niloticus (l.)×o. aureus (steindachner). a series of immersion challenge trials were performed using isolates of the five established genomovars o ... | 2015 | 24909885 |
| new attenuated vaccine against columnaris disease in fish: choosing the right parental strain is critical for vaccine efficacy. | flavobacterium columnare, the causative agent of columnaris disease, is a highly diverse species comprised by three genomovars. genomovar ii strains are more virulent toward catfishes than genomovar i isolates. the objective of this study was to compare the vaccine efficacy of avirulent mutants derived from genomovars i and ii using a rifampicin-resistance strategy. first, we compared the efficacy of 13 genomovar ii mutants in channel catfish (ictalurus punctatus) fingerlings and identified muta ... | 2013 | 24012568 |
| sensitivity and specificity of real-time pcr and bacteriological culture for francisellosis in farm-raised nile tilapia (oreochromis niloticus l.). | despite the worldwide occurrence of francisella noatunensis subsp. orientalis (fno) infection in farmed tilapia, sensitivity and specificity estimates of commonly used diagnostic tests have not been reported. this study aimed to estimate the sensitivity and specificity of bacteriological culture and qpcr to detect fno infection. we tested 559 fish, sampled from four farms with different epidemiological scenarios: (i) healthy fish in a hatchery free of fno; (ii) targeted sampling of diseased fish ... | 2016 | 27670740 |
| natural coinfection by streptococcus agalactiae and francisella noatunensis subsp. orientalis in farmed nile tilapia (oreochromis niloticus l.). | streptococcus agalactiae and francisella noatunensis subsp. orientalis (fno) are important pathogens for farm-raised tilapia worldwide. there are no reports of coinfection caused by s. agalactiae and fno in fish. this study aimed to determine the aetiology of atypical mortalities in a cage farm of nile tilapia and to characterize the genetic diversity of the isolates. fifty-two fish were sampled and subjected to parasitological and bacteriological examination. the s. agalactiae and fno isolates ... | 2017 | 27144661 |
| outbreaks and genetic diversity of francisella noatunensis subsp orientalis isolated from farm-raised nile tilapia (oreochromis niloticus) in brazil. | francisella noatunensis subsp orientalis (fno) is an emerging pathogen of warm water tilapia in a number of different countries. the disease caused by this bacterium in fish is characterized by a systemic granulomatous infection that causes high mortality rates during outbreaks. fno has been previously described in asia, europe, and central and north america. its occurrence in south america has never been described. since 2012, outbreaks of a granulomatous disease have been recorded in cage farm ... | 2014 | 25117328 |
| francisella noatunensis subsp. orientalis pathogenesis analyzed by experimental immersion challenge in nile tilapia, oreochromis niloticus (l.). | francisella noatunensis subsp. orientalis (fno) (syn. f. asiatica) is an emergent warmwater fish pathogen and the causative agent of francisellosis in tilapia (oreochromis sp). to study the pathogenesis of this bacterium, tilapia fingerlings were experimentally infected by immersion challenge with wild type (wt) fno and the distribution of bacteria to multiple organs, as well as associated lesion development, investigated after 3, 24, 48, 96, and 192h by real-time pcr and histopathological exami ... | 2013 | 23415477 |
| effects of temperature and salt concentration on francisella noatunensis subsp. orientalis infections in nile tilapia oreochromis niloticus. | little is known about the environmental conditions that allow francisella noatunensis subsp. orientalis, a worldwide emergent bacterial fish pathogen, to colonize and infect wild and cultured fish. we evaluated the effect of temperature and salinity on the infectivity of f. noatunensis subsp. orientalis in nile tilapia oreochromis niloticus (l). immersion challenges of tilapia with f. noatunensis subsp. orientalis at water temperatures of 25 and 30°c in both sea and fresh water were conducted fo ... | 2012 | 23324418 |
| inflammatory responses of nile tilapia oreochromis niloticus to streptococcus agalactiae: effects of vaccination and yeast diet supplement. | this study evaluated the effects of dietary supplementation with 0.3% saccharomyces cerevisiae yeast cell wall and of vaccination against streptococcus agalactiae on the cellular component of acute inflammation induced in the coelomic cavity of nile tilapia oreochromis niloticus and on survival of the fish after challenge. a total of 84 tilapia of mean (±sd) weight 125.0 ± 1.5 g were distributed among twelve 310 l fiberglass tanks according to a 2 × 2 × 3 factorial design in the following manner ... | 2012 | 22535873 |
| efficacy of florfenicol for control of mortality associated with francisella noatunensis subsp. orientalis in nile tilapia, oreochromis niloticus (l.). | francisella noatunensis subsp. orientalis (fno) (syn. f. asiatica) is an emergent gram-negative facultative intracellular bacterium. although it is considered one of the most pathogenic bacteria in fish, there are no commercially available treatments or vaccines. the objective of this project was to determine the most efficacious concentration of florfenicol (ffc) [10, 15 or 20 mg ffc kg(-1) body weight (bw) per days for 10 days] administered in feed to control experimentally induced infections ... | 2013 | 23134104 |
| gyrodactylus malalai sp. nov. (monogenea, gyrodactylidae) from nile tilapia, oreochromis niloticus (l.) and redbelly tilapia, tilapia zillii (gervais) (teleostei, cichlidae) in the lake turkana, kenya. | gyrodactylus malalai sp. nov. is described from the fin surface of cichlid fishes oreochromis niloticus (l.) and tilapia zillii (gervais) caught in lake turkana (kenya). the new species morphologically resembles gyrodactylus nyanzae paperna, 1973, but can be readily distinguished by the shape of the marginal hook sickles and the size of its hamuli. the sequence data of rdna spanning partial 18s, internal transcribe spacer 1 and 2 and the 5.8s gene is unique within genbank. genetically, as most s ... | 2012 | 22807048 |
| quality properties, fatty acids, and biogenic amines profile of fresh tilapia stored in ice. | this work determines quality properties and fatty acids content of nile tilapia (oreochromis niloticus) stored in ice for 21 d. the quality properties consist of thiobarbituic acid (tba), total volatile basic nitrogen (tvb-n), trimethylamine (tma), and microbiological analysis (total viable count (tvc), total coliform, salmonella and staphylococcus aureus) and determination of biogenic amines content (histamine, cadaverine, putrescine, spermine, spermidine, 2-phenylethylamine, agmatine, tyramine ... | 2013 | 23865452 |
| megalocytivirus infection in cultured nile tilapia oreochromis niloticus. | megalocytiviruses, such as infectious spleen and kidney necrosis virus (isknv), induce lethal systemic diseases in both ornamental and food fish species. in this study, we investigated an epizootic affecting nile tilapia oreochromis niloticus cultured in the us midwest. diseased fish displayed lethargy, gill pallor, and distension of the coelomic cavity due to ascites. histopathological examination revealed a severe systemic abundance of intravascular megalocytes that were especially prominent i ... | 2016 | 27225209 |
| effect of dietary probiotic supplementation on intestinal microbiota and physiological conditions of nile tilapia (oreochromis niloticus) under waterborne cadmium exposure. | the heavy metal cadmium (cd) is a hazardous pollutant that exerts various toxic effects on aquatic animals. the biomagnifying effects of this non-essential element in the food chain also pose threats to human health. in this study, the protective effect of a dietary probiotic supplementation, lactobacillus plantarum ccfm8610, on the intestinal microbiota and physiological conditions of nile tilapia (oreochromis niloticus) exposed to waterborne cd was evaluated. two hundred fish were divided into ... | 2017 | 28028640 |
| combined administration of low molecular weight sodium alginate boosted immunomodulatory, disease resistance and growth enhancing effects of lactobacillus plantarum in nile tilapia (oreochromis niloticus). | the present study investigates the effects of combined or singular administration of low molecular weight sodium alginate (lwmsa) and lactobacillus plantarum on innate immune response, disease resistance and growth performance of nile tilapia (oreochromis niloticus). three hundred and twenty fish were supplied and randomly stocked in sixteen glass tanks (150 l) assigned to four treatments as follows: 0 g kg(-1) lmwsa (control, diet 1), 10 g kg(-1) lmwsa (diet 2), 10(8) cfu g(-1)l. plantarum (die ... | 2016 | 27737798 |