| differential specificity of two insecticidal toxins from bacillus thuringiensis var. aizawai. | bacillus thuringiensis var. aizawai hd-249 produces more than one protein of 130-135 kd in its insecticidal crystal delta-endotoxin. we describe an indirect method of assessing the relative contribution to toxicity of two of these protoxins using monospecific antibodies directed against their active proteolytic products. our results show that one toxin is active against spodoptera frugiperda but not choristoneura fumiferana cells in vitro, while the other lyses c. fumiferana but not s. frugiperd ... | 1988 | 2835588 |
| functional analysis of the p10 gene 5' leader sequence of the autographa californica nuclear polyhedrosis virus. | deletions were made in the 5' non-coding (leader) sequence of the autographa californica nuclear polyhedrosis virus (acnpv) p10 gene which progressively removed nucleotides upstream from the atg translation initiation codon. the effect of these deletions on p10 gene expression was studied using a transient expression assay. fragments containing the putative promoter and the entire or partly deleted 5' leader sequence of the p10 gene were inserted in front of the chloramphenicol acetyltransferase ... | 1988 | 2836797 |
| synthesis of functional human immunodeficiency virus tat protein in baculovirus as determined by a cell-cell fusion assay. | the human immunodeficiency virus tat protein is a strong trans-activator of the expression of mrnas originating from the viral long terminal repeat. we have expressed the first 72 amino acids (coding exon 1) of this protein in eucaryotic spodoptera frugiperda sf9 cells by using a baculovirus vector, autographa californica nuclear polyhedrosis virus. we show that the baculovirus vector stably produced the 72-amino-acid form of the tat protein but was unable to stably synthesize a larger 101-amino ... | 1988 | 2843682 |
| bluetongue virus tubules made in insect cells by recombinant baculoviruses: expression of the ns1 gene of bluetongue virus serotype 10. | bluetongue virus (btv) forms tubules in mammalian cells. these tubules appear to be composed of only one type of protein, ns1, a major nonstructural protein of the virus. to obtain direct evidence for the origin of the tubules, the complete m6 gene of btv serotype 10 was inserted into the baculovirus transfer vector pacym1, so that it was under the control of the polyhedrin promoter of autographa californica nuclear polyhedrosis virus. after cotransfection of spodoptera frugiperda cells with wil ... | 1988 | 2845115 |
| overproduction of the protein encoded by the maize transposable element ac in insect cells by a baculovirus vector. | the polypeptide encoded in the activator (ac) element of zea mays l. has been expressed in spodoptera frugiperda insect cells using plasmids which carry the strong polyhedrin promoter of the baculovirus autographa californica nuclear polyhedrosis virus (acnpv). recombinant acnpvs with the ac-cdna integrated and under the control of the viral polyhedrin promoter have been isolated and their genomes have been partly characterized as to the location of the foreign dna insert. upon infection of s. f ... | 1988 | 2851092 |
| expression of human tyrosine hydroxylase cdna in invertebrate cells using a baculovirus vector. | a human cdna containing the complete coding sequence for a human tyrosine hydroxylase (ec 1.14.16.2, form 2) was introduced into the genome of autographa californica nuclear polyhedrosis virus (acnpv) downstream to the polyhedrin promoter. infection of spodoptera frugiperda cells (sf9) with recombinant virus resulted in the expression of human tyrosine hydroxylase in these invertebrate cells. characterization of tyrosine hydroxylase activity in infected sf9 cells demonstrated both substrate and ... | 1988 | 2896667 |
| segment w of campoletis sonorensis virus: expression, gene products, and organization. | campoletis sonorensis virus (csv, polydnaviridae) is a segmented double-stranded dna virus which has an apparently symbiotic relationship with the parasitic wasp, campoletis sonorensis. csv replicates in the oviducts of the parasitic wasp and is injected into the wasp's host, heliothis virescens (lepidoptera; noctuiidae), during oviposition. in the parasitized lepidopteran host, the virus has a dramatic effect on host physiology and viral gene products are believed to play an essential role in t ... | 1989 | 2922929 |
| identification of the n and nss proteins coded by the ambisense s rna of punta toro phlebovirus using monospecific antisera raised to baculovirus expressed n and nss proteins. | an essentially complete dna copy of the ambisense s rna species of punta toro (pt) phlebovirus (t. ihara, h. akashi, and d.h.l. bishop, 1984, virology 136, 293-306) has been inserted in either orientation into autographa californica nuclear polyhedrosis baculovirus (acnpv) in lieu of the 5' coding region of the acnpv polyhedrin gene (g.e. smith, m.d. summers, and m.j. fraser, 1983, mol. cell. biol. 3, 2156-2165). the two types of recombinant viruses were used to infect spodoptera frugiperda cell ... | 1987 | 2950650 |
| active human erythropoietin expressed in insect cells using a baculovirus vector: a role for n-linked oligosaccharide. | biologically active recombinant human erythropoietin has been expressed at high levels in an insect cell background. expression involved the preparation of a human erythropoietin cdna, the transfer of this cdna to the autographa californica nuclear polyhedrosis virus (acnpv) genome under the polyhedrin gene promoter, and the subsequent infection of spodoptera frugiperda cells with recombinant acnpv. erythropoietin cdna was prepared through the expression of the human erythropoietin gene in cos c ... | 1987 | 2960381 |
| purification and functional properties of simian virus 40 large and small t antigens overproduced in insect cells. | the insect baculovirus autographa californica nuclear polyhedrosis virus was used as an expression vector for the simian virus 40 (sv40) small t (t) and large t (t) antigens. spodoptera frugiperda (sf9) cells infected with recombinant viruses encoding these proteins produced approximately 1 to 2 micrograms of t and up to 30 micrograms of t per 3 x 10(6) cells. the former was highly soluble after nonidet p-40 extraction of the infected cells, unlike its escherichia coli-produced counterpart. both ... | 1988 | 2969056 |
| immunization of mice with dengue structural proteins and nonstructural protein ns1 expressed by baculovirus recombinant induces resistance to dengue virus encephalitis. | we have constructed a recombinant baculovirus containing a 4.0-kilobase dengue virus cdna sequence that codes for the three virus structural proteins, capsid (c) protein, premembrane (prem) protein, and envelope glycoprotein (e), and nonstructural proteins ns1 and ns2a. infection of cultured spodoptera frugiperda cells with this recombinant virus resulted in the production of e and ns1 proteins that were similar in size to the corresponding viral proteins expressed in dengue virus-infected simia ... | 1988 | 2969058 |
| specificity of bacillus thuringiensis var. colmeri insecticidal delta-endotoxin is determined by differential proteolytic processing of the protoxin by larval gut proteases. | the native crystal delta-endotoxin produced by bacillus thuringiensis var. colmeri, serotype 21, is toxic to both lepidopteran (pieris brassicae) and dipteran (aedes aegypti) larvae. solubilization of the crystal delta-endotoxin in alkaline reducing conditions and activation with trypsin and gut extracts from susceptible insects yielded a preparation whose toxicity could be assayed in vitro against a range of insect cell lines. after activation with aedes aegypti gut extract the preparation was ... | 1986 | 3009187 |
| expression of the influenza virus haemagglutinin in insect cells by a baculovirus vector. | the insect baculovirus autographa californica nuclear polyhedrosis virus (acnpv) has played a major role in studies on the molecular biology of insect dna viruses. recently, this system has been effectively adapted as a highly efficient vector in insect cells for the expression of several mammalian genes. a cdna sequence of the influenza (fowl plague) virus haemagglutinin gene has been inserted into the bamhi site of the pac373 polyhedrin vector. spodoptera frugiperda cells were co-transfected w ... | 1986 | 3015601 |
| abundant synthesis of functional human t-cell leukemia virus type i p40x protein in eucaryotic cells by using a baculovirus expression vector. | the human t-cell leukemia virus type i (htlv-i) p40x protein is a 40-kilodalton polypeptide encoded in the 3'-terminal region of the virus. this protein is responsible for positive transcriptional trans-activation of promoter elements located within the htlv-i long terminal repeat. we introduced the protein-coding region of htlv-i p40x into the genome of the baculovirus autographa californica nuclear polyhedrosis virus. after infection of the insect spodoptera frugiperda (sf9) cell line, this re ... | 1987 | 3027397 |
| production and characterization of the neutralization antigen vp2 of bluetongue virus serotype 10 using a baculovirus expression vector. | dna representing rna segment 2 of bluetongue virus (btv) serotype 10, corresponding to the gene that codes for the btv neutralization antigen vp2, has been inserted into a baculovirus transfer vector in lieu of the 5' coding region of the polyhedrin gene of autographa californica nuclear polyhedrosis virus (acnpv). after cotransfection of spodoptera frugiperda cells with wild-type acnpv dna in the presence of the derived recombinant transfer vector dna, polyhedrin-negative recombinant baculoviru ... | 1987 | 3029984 |
| synthesis and immunogenicity of the rotavirus major capsid antigen using a baculovirus expression system. | rotaviruses are the major pathogens that cause life-threatening diarrhea in young children and animals. we inserted a simian rotavirus sa11 gene 6 cdna into the genome of the baculovirus autographa californica nuclear polyhedrosis virus adjacent to the strong polyhedrin promoter. the major capsid antigen (vp6) was expressed in high yields (20 to 150 micrograms/10(6) cells) when spodoptera frugiperda cells were infected with baculovirus recombinants containing sa11 gene 6 inserts. reactivity with ... | 1987 | 3033276 |
| a baculovirus vector can express intron-containing genes. | a simjan virus 40 genomic fragment containing the genes coding for the large t and small t antigens was inserted into the genome of the baculovirus autographa californica nuclear polyhedrosis virus downstream of the strong polyhedrin promoter. infection of eucaryotic spodoptera frugiperda (sf9) cells with this recombinant virus produced significant amounts of small t antigen and little or no large t protein. analysis by northern blotting and s1 nuclease digestion revealed correct and preferentia ... | 1987 | 3033307 |
| expression of bluetongue virus group-specific antigen vp3 in insect cells by a baculovirus vector: its use for the detection of bluetongue virus antibodies. | dna representing rna segment 3 of bluetongue virus (btv) serotype 17, corresponding to the gene that codes for a group-specific antigen vp3, has been inserted into a baculovirus transfer vector in lieu of the 5' coding region of the polyhedrin gene of autographa californica nuclear polyhedrosis virus (acnpv). after cotransfection of spodoptera frugiperda cells with wild-type acnpv dna in the presence of the derived recombinant transfer vector dna, polyhedrin-negative recombinant baculoviruses we ... | 1987 | 3035063 |
| resistance of the 64k protein of budded autographa californica nuclear polyhedrosis virus to functional inactivation by proteolysis. | the 64k surface protein of budded autographa californica nuclear polyhedrosis virus (acmnpv bv) is known to play a role in the functional entry of acmnpv bv into spodoptera frugiperda iplb-sf-21 cells by adsorptive endocytosis. acv1, a neutralizing monoclonal antibody, reacts with the 64k protein and in doing so prevents efficient entry. in this communication we report that treatment of acmnpv bv with either trypsin or proteinase k cleaves the 64k protein into one major fragment of 34.6k and two ... | 1988 | 3046120 |
| co-expression of the hepatitis b surface and core antigens using baculovirus multiple expression vectors. | the hepatitis b (hb) virus dna sequences coding for the pre-core (prec) or c antigens (hbpcag, hbcag) have been inserted into the baculovirus plasmid transfer vector, pacym1, such that the hb viral sequences are under the control of the polyhedrin promoter of autographa californica nuclear polyhedrosis virus (acnpv). spodoptera frugiperda cells infected with either of the derived recombinant plasmids in the presence of infectious acnpv dna yielded recombinant, polyhedrin-negative viruses that ex ... | 1988 | 3053987 |
| association of autographa californica nuclear polyhedrosis virus (acmnpv) with the nuclear matrix. | nuclear matrices from uninfected spodoptera frugiperda cells and those infected with autographa californica nuclear polyhedrosis virus (acmnpv) were isolated and their protein constituents were compared. proteins were characterized according to size and several different antibodies to drosophila nuclear proteins were employed in an attempt to identify the proteins comprising this nuclear substructure. three species of lamins were identified as major constituents of the nuclear matrix of spodopte ... | 1988 | 3055664 |
| baculovirus transcription in the presence of inhibitors and in nonpermissive drosophila cells. | regulation of transcription within four well-defined regions of the dna genome of the baculovirus autographa californica nuclear polyhedrosis virus (acnpv) was studied in a permissive lepidopteran cell line. spodoptera frugiperda iplb-sf-21 and a nonpermissive dipteran cell line, drosophila melanogaster dl-1. cycloheximide, an inhibitor of protein synthesis, was used to identify immediate early transcripts and aphidicolin, an effective inhibitor of dna replication, was used to distinguish early ... | 1986 | 3099497 |
| functional studies on the p10 gene of autographa californica nuclear polyhedrosis virus using a recombinant expressing a p10-beta-galactosidase fusion gene. | the beta-galactosidase gene (lacz) of escherichia coli was inserted in phase with the coding sequence of the autographa californica nuclear polyhedrosis virus (acmnpv) late-expressed mr 10,000 (p10) gene. the fusion gene was inserted into the acmnpv genome by cotransfection of a recombinant plasmid pacr159z, consisting of the ecori p fragment-containing pbr325-derived plasmid pacr159 and the lacz insert in the p10 gene, and wild-type acmnpvdna. infection of spodoptera frugiperda cells by the res ... | 1988 | 3128641 |
| signals important for high-level expression of foreign genes in autographa californica nuclear polyhedrosis virus expression vectors. | the transcriptional and translational signals required for efficient expression of the chloramphenicol acetyltransferase, beta-galactosidase, and tissue plasminogen activator genes, under the control of the polyhedrin promoter in spodoptera frugiperda cells infected with autographa californica nuclear polyhedrosis virus, were investigated by sds-page and rna dot blot analysis. the recombinant baculoviruses all contained alterations in the leader sequence or 5' proximal coding region of the polyh ... | 1988 | 3142147 |
| human tissue-type plasminogen activator synthesized by using a baculovirus vector in insect cells compared with human plasminogen activator produced in mouse cells. | a cdna fragment encoding the human tissue-type plasminogen activator was inserted into the baculovirus autographa californica nuclear polyhedrosis virus downstream from the polyhedrin promoter. the induction kinetics of t-pa was followed, after infection of spodoptera frugiperda cells, at both mrna and protein levels. fibrinolytically active plasminogen activator accumulated in the culture medium and reached 2.5 micrograms/ml after 120 h. the protein was compared with recombinant plasminogen act ... | 1988 | 3149610 |
| toxicity of selected tremorgenic mycotoxins and related compounds to spodoptera frugiperda and heliothis zea. | a series of tremorgenic mycotoxins and related compounds were tested for oral toxicity to the fall armyworm (spodoptera frugiperda) and corn earworm (heliothis zea) by incorporation of materials into artificial diets and examining mortality and weights after 7 days. significant mortality to both insect species was caused with dihydroxyaflavinine and roseotoxin b, while significant mortality to h. zea was also caused by penitrem a at 25 ppm. after 7 days, weighs of larvae treated with 25 ppm peni ... | 1988 | 3209479 |
| expression of snowshoe hare bunyavirus s rna coding proteins by recombinant baculoviruses. | recombinant baculoviruses have been constructed that express the two snowshoe hare (ssh) bunyavirus proteins coded in overlapping reading frames of the ssh s viral-complementary rna species (namely the nucleoprotein, n, and the nonstructural protein, nss). the 26.5 kda n protein, which is read from the first aug of the mrna containing the ssh s sequence, was expressed at a high level (estimated to be ca 40% of the stained cellular proteins in recombinant baculovirus-infected spodoptera frugiperd ... | 1988 | 3223063 |
| synthesis of a gene coding for an insect-specific scorpion neurotoxin and attempts to express it using baculovirus vectors. | we have explored the possibility of improving baculovirus pesticides by incorporating an insect-specific neurotoxin gene into a baculovirus genome. a 112-bp gene (beit) encoding insectotoxin-1 of the scorpion buthus eupeus was synthesized and cloned in escherichia coli. for expression, beit was transferred to the dna genome of autographa californica nuclear polyhedrosis virus (acmnpv). three different recombinant acmnpvs, carrying beit under the control of the strong acmnpv polyhedrin promoter, ... | 1988 | 3243439 |
| glycosylation and processing of high levels of active human glucocerebrosidase in invertebrate cells using a baculovirus expression vector. | a human cdna containing the complete coding region for the lysosomal glycoprotein glucocerebrosidase (ec 3.2.1.45) was introduced into the genome of autographa californica nuclear polyhedrosis virus downstream from the polyhedrin promoter. infection of spodoptera frugiperda cells (sf9) with recombinant virus produced high levels of glucocerebrosidase, 40% of which was in the culture medium. the amino-terminal amino acid sequence of the recombinantly produced enzyme was identical to that of matur ... | 1988 | 3282855 |
| secretion of particles of hepatitis b surface antigen from insect cells using a baculovirus vector. | the coding sequences of the hepatitis b virus surface antigen were inserted into a baculovirus transfer vector produced from autographa californica nuclear polyhedrosis virus (acnpv) so that the foreign gene was under the control of the acnpv polyhedrin promoter. spodoptera frugiperda cells infected with the derived recombinant baculovirus produced and secreted 22 nm particles containing the hepatitis b surface antigen. the particles had morphological and antigenic properties identical to those ... | 1987 | 3312490 |
| in vivo and in vitro host range of autographa californica nuclear polyhedrosis virus and spodoptera frugiperda nuclear polyhedrosis virus. | | 1987 | 3320215 |
| characterization of the toxicity and cytopathic specificity of a cloned bacillus thuringiensis crystal protein using insect cell culture. | an insecticidal protein gene from bacillus thuringiensis var. aizawai was cloned in escherichia coli. the cloned gene expressed at a high level and the synthesized protein appeared as an insoluble, phase-bright inclusion in the cytoplasm. these inclusions were isolated by density gradient centrifugation, the isolated protein was activated in vitro by different proteolytic regimes and the toxicity of the resulting preparations was studied using insect cells grown in tissue culture. the inclusions ... | 1987 | 3330756 |
| the development of multiple expression vectors for high level synthesis of eukaryotic proteins: expression of lcmv-n and acnpv polyhedrin protein by a recombinant baculovirus. | a copy of the polyhedrin gene promoter of autographa californica nuclear polyhedrosis virus (acnpv), in association with the coding region of lymphocytic choriomeningitis virus n protein (lcmv-n) and the relevant polyhedrin transcription termination signals, has been cloned into the unique ecorv site of a plasmid representing an ecori derived fragment of the acnpv genome. the cloning site is upstream (but in the opposite orientation) of the natural acnpv polyhedrin gene. the derived pacvc2 trans ... | 1987 | 3334094 |
| studies on autographa californica nuclear polyhedrosis virus replication in spodoptera littoralis cells including virus-induced protein synthesis. | the replication of the autographa californica nuclear polyhedrosis virus in spodoptera littoralis cells has been investigated. various cytopathic changes were detected by light and electron microscopy and progeny enveloped virus particles, some occluded within polyhedra, were later seen in the nucleus of infected cells. infectious virus was released into the growth medium and increased exponentially from ca. 10 to 24 hours post infection and then slowly increased over the next 4 days. in compari ... | 1986 | 3510608 |
| changes in the nucleoprotein complexes of a baculovirus dna during infection. | the nature of the dna-protein complexes assumed by autographa californica nuclear polyhedrosis virus (acnpv) dna during infection of spodoptera frugiperda cells was investigated by micrococcal nuclease digestion of infected nuclei. both parental viral dna and progeny viral dna assumed a chromatin-like structure early in infection. by late times (24 hr) p.i., the viral dna acquired a unique nucleoprotein structure. in addition to fragments of mononucleosome size (185 bp), two subnucleosomal bands ... | 1986 | 3518228 |
| expression of the s-coded genes of lymphocytic choriomeningitis arenavirus using a baculovirus vector. | a dna copy of the lymphocytic choriomeningitis virus (lcmv, we strain)s rna species has been inserted in both orientations into plasmids containing a 7.1 kb dna sequence of the baculovirus autographa californica nuclear polyhedrosis virus (acnpv). the inserts were placed behind the polyhedrin gene promoter of acnpv and in the place of the nucleotide sequences including and flanking the polyhedrin gene translation initiation codon. the derived plasmids were used to obtain recombinant acnpv viruse ... | 1986 | 3525745 |
| two of the three influenza viral polymerase proteins expressed by using baculovirus vectors form a complex in insect cells. | each of the influenza virus polymerase (p) genes pb1, pb2, and pa was inserted into a baculovirus vector under the control of the polyhedrin promoter. in insect (spodoptera frugiperda) cells infected by each baculovirus recombinant containing a p gene insert, a large amount of the encoded p protein was synthesized. gel electrophoretic analysis of the total proteins in infected cells revealed the presence of a new protein band corresponding to the encoded p protein that was abundant enough to be ... | 1987 | 3543398 |
| baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins. | the requirements for high level expression of three foreign proteins using the polyhedrin gene promoter of autographa californica nuclear polyhedrosis virus (acnpv, baculoviridae) have been investigated. in spodoptera frugiperda cells infected with the appropriate recombinant baculoviruses, the synthesis of the two s rna coded genes of lymphocytic choriomeningitis virus (lcmv; i.e. the nucleoprotein, n, and glycoprotein precursor, gpc), or the haemagglutinin gene of influenza a virus, appears to ... | 1987 | 3553425 |
| fatty acid composition of whole bodies, specific tissues and cell lines of two lepidopteran insects. | we detail the fatty acid compositions of last larval instars of two lepidopterans, spodoptera frugiperda and trichoplusia ni, two tissues from t. ni, a cell line derived from each species and the respective larval and cell culture media. larval whole-body and specific tissue fatty acid profiles exhibited the major features commonly found in previous lepidopteran analyses, whereas the cell-line fatty acid compositions were substantially different from the compositions of both their growth media a ... | 1986 | 3780187 |
| structural and functional similarities between the major hemolymph protein of fall armyworm and cat c4 binding protein of the complement system. | we have investigated the structural and functional similarities between insect (armyworm) major larval serum protein (lsp-i) and mammalian (cat) c4 binding protein (i) by comparing the major chymotryptic peptide patterns using sodium dodecyl sulfate polyacrylamide gel electrophoresis and (ii) their ability to prevent assembly of classical c3 convertase (eac142). both cat c4 bp and spodoptera lsp-i are composed of identical subunits of mr = 75,000 and mr = 80,000, respectively and generate the ch ... | 1985 | 3850057 |
| infectivity assays for the autographa californica nuclear polyhedrosis virus using spodoptera littoralis cells. | tissue culture id50 and plaque assays for the autographa californica nuclear polyhedrosis virus, using the spodoptera littoralis cell line hpb-sl, were developed. direct comparison using these assay methods showed that these cells were as susceptible to infection as the more commonly used spodoptera frugiperda cell line iplb-sf-21. both infectious tissue culture supernatants or virus isolated directly from polyhedra could be titrated. it was important to use low cell seeding densities in the ass ... | 1985 | 3882730 |
| the promoter of the late p10 gene in the insect nuclear polyhedrosis virus autographa californica: activation by viral gene products and sensitivity to dna methylation. | in lepidopteran insect cells infected with the baculovirus autographa californica nuclear polyhedrosis virus (acnpv), two major late viral gene products are expressed: the polyhedrin, a 28 000 mol. wt. protein which makes up the mass of the nuclear inclusion bodies, and a 10 000 mol. wt. protein (p10) whose function is unknown. the nucleotide sequences of these strong promoters conform to those of other eukaryotic promoters and are rich in at base pairs. we used the psvo-cat construct containing ... | 1985 | 3891327 |
| isolation of large and small plaque variants of the autographa californica nuclear polyhedrosis virus. brief report. | variants of the autographa californica nuclear polyhedrosis virus which produce large (1.4 mm) or small (0.5 mm) plaques on spodoptera littoralis cells have been isolated. yields of extracellular virus and polyhedra by the large plaque variant were six-fold higher and 140-fold lower, respectively, than those obtained with the small plaque variant. however the two variants could not be distinguished when spodoptera frugiperda cells were used. | 1985 | 3893392 |
| growth inhibitory, insecticidal and antifeedant effects of some antileukemic and cytotoxic quassinoids on two species of agricultural pests. | several quassinoids, obtained by isolation and derivatization from simaba multiflora and soulamea soulameoides, were evaluated for growth inhibitory and insecticidal effects against the tobacco budworm (heliothis virescens) and for antifeedant effects against h. virescens and the fall armyworm (spodoptera frugiperda). the relative activity of the quassinoids as insect growth inhibitors generally paralleled their known relative potency as antileukemic and cytotoxic agents. | 1985 | 3972085 |
| in vitro host range of five baculoviruses in lepidopteran cell lines. | the in vitro host range of five nuclear polyhedrosis viruses (npv) was assessed in five lepidopteran cell lines from three genera. multiple-enveloped baculoviruses of autographa californica (acmnpv), trichoplusia ni (tnmnpv), and galleria mellonella (gmmnpv) replicated in cells of t. ni, spodoptera frugiperda, and heliothis virescens to a titer of approximately 10(7) tcid50/ml. the multiple-enveloped baculovirus of s. frugiperda (sfmnpv) replicated only in s. frugiperda cells. the single-envelop ... | 1985 | 3988484 |
| hemagglutinin activity in the hemolymph of anticarsia gemmatalis larvae infected with the fungus nomuraea rileyi. | hemolymph samples from larvae of 3 lepidopteran species (anticarsia gemmatalis, trichoplusia ni and spodoptera frugiperda) were tested for hemagglutination activity. samples from a. gemmatalis larvae which had been injected 12-24 hrs previously with hyphal bodies of the entomopathogenic fungus nomuraea rileyi showed agglutination activity against human 0, rabbit and sheep erythrocytes. little or no activity was detected in samples from the other 2 larval species. low titers (approximately 1:2) w ... | 1985 | 3996705 |
| fatty acid profiles of cells from the insect cell line iprl-21 (spodoptera frugiperda) and of the tissue culture medium after repeated use. | | 1973 | 4353540 |
| some genetic aspects of the resistance of spodoptera frugiperda to a nuclear polyhedrosis virus. | | 1974 | 4597766 |
| replication of a nuclear polyhedrosis virus in a continuous cell culture of spodoptera frugiperda: purification, assay of infectivity, and growth characteristics of the virus. | nonoccluded virus, polyhedra, and occluded virus were purified from a continuous cell culture of spodopera frugiperda infected with nuclear polyhedrosis virus. the optimal temperature for the replication and lateral transmission of infectivity for the nuclear polyhedrosis viruses (npv) in cell culture was 27 c. end-point dilution and plaque assay procedures for the measurement of infectivity are described and compared. dose-response data demonstrated that a single particle could initiate an infe ... | 1974 | 4608299 |
| characterization of deoxyribonucleic acid isolated from the granulosis viruses of the cabbage looper, trichoplusia ni and the fall armyworm, spodoptera frugiperda. | | 1972 | 4636117 |
| characterization of nuclear polyhedrosis virus dnas. | the nuclear polyhedrosis virus dnas characterized and compared in this study consist of the singly-enveloped nucleocapsids (snpv) of trichoplusia ni and the bundles of nucleocapsids common to a single envelope (mnpv) from spodoptera frugiperda and rachiplusia ou. the snpv and mnpv dnas are very similar in hydrodynamic properties and molecular weights. in addition, the npv dnas are similar in size to those extracted from the granulosis viruses that infect t. ni and s. frugiperda. as isolated from ... | 1973 | 4761726 |
| flight performance and metabolism of the moth spodoptera frugiperda. | | 1971 | 5158369 |
| sex pheromones of the southern armyworm moth: isolation, identification, and synthesis. | two sex pheromones have been isolated from the female southern armyworm moth, prodenia eridania (cramer), and identified as cis-9-tetradecen-1-ol acetate, identical with the sex pheromone of the fall armyworm moth, spodoptera frugiperda (j. e. smith), and cis-9,trans-12-tetradecadien-1-ol acetate. | 1970 | 5507205 |
| comparative histopathology of a granulosis and a nuclear polyhedrosis of spodoptera frugiperda. | | 1968 | 5655351 |
| synthesis of cis-9-tetradecen-1-ol acetate, the sex pheromone of the fall armyworm. | | 1968 | 5690959 |
| transcription of overlapping sets of rnas from the genome of autographa californica nuclear polyhedrosis virus: a novel method for mapping rnas. | the insect baculovirus autographa californica nuclear polyhedrosis virus (acnpv) contains a double-stranded, supercoiled circular genome of 126 to 129 kilobase pairs in length. in cultured spodoptera frugiperda insect cells the virus replications, and early and late phases of viral genome expression are discernible. we previously mapped 5 early and at least 32 late different viral polypeptides on the viral genome (h. esche, h. lübbert, b. siegmann, and w. doerfler, embo j. 1:1629-1633, 1982). ho ... | 1984 | 6090702 |
| chemically-defined media for production of insect cells and viruses in vitro. | two chemically-defined media are described. they support the growth of a) an established cell line of spodoptera frugiperda cells and b) two established mosquito cell lines from aedes aegypti and anopheles gambiae. the replication of autographa californica nuclear polyhedrosis virus (acnpv) in s. frugiperda cells grown in a defined medium is reported. | 1980 | 6102531 |
| microsomal biphenyl hydroxylase of fall armyworm larvae and its induction by allelochemicals and host plants. | biphenyl was hydroxylated to 4-hydroxybiphenyl by a mixed-function oxidase system from midgut microsomes of fall armyworm (spodoptera frugiperda) larvae. optimum conditions for assaying the enzyme were established. the microsomal biphenyl 4-hydroxylase activity increased during larval development and declined in the prepupa. allelochemicals (monoterpenes, indoles and flavones), drugs (phenobarbital and 3-methylcholanthrene) and host plants were found to induce the hydroxylase. the enzyme was als ... | 1984 | 6146463 |
| analysis of a mutant of autographa california nuclear polyhedrosis virus with a defect in the morphogenesis of the occlusion body macromolecular lattice. | a mutant (m-29) of autographa californica nuclear polyhedrosis virus (acmnpv) grew in spodoptera frugiperda and trichoplusia ni cells but did not form typical intranuclear occlusion bodies (ob); instead, small particles (95 to 180 nm diam.) were produced in copious amounts within nuclei. ultrastructural studies showed that the particles did not occlude enveloped nucleocapsids and that they lacked a macromolecular paracrystalline lattice and a structure equivalent to the occlusion body envelope. ... | 1983 | 6306151 |
| the translational map of the autographa californica nuclear polyhedrosis virus (acnpv) genome. | we have mapped early and late viral gene products expressed in autographa californica nuclear polyhedrosis virus ( acnpv )-infected spodoptera frugiperda cells by cell-free translation of virus-specific rna which was selected by hybridization to cloned restriction endonuclease fragments of acnpv dna. proteins synthesized in vitro were labeled with [35s]methionine and analyzed by sds-polyacrylamide gel electrophoresis followed by fluorography. at least four early acnpv -specific polypeptides were ... | 1982 | 6327284 |
| physical maps of sfmnpv baculovirus dna and its genomic variants. | spodoptera frugiperda mnpv was plaque-purified, and the viral dna from the plaque-purified isolates was analyzed with restriction endonuclease enzymes. seven distinct variants were identified when the dna of the isolates were analyzed by ecori and hindiii. the dnas of the sfmnpv predominant type (prototype) and the variants were mapped with bamhi, bglii, bsteii, ecori, hindiii, kpni, and psti by multiple enzyme digestion and blot hybridization. the cleavage sites generated by the seven restricti ... | 1984 | 6330992 |
| monoclonal antibodies to baculovirus structural proteins: determination of specificities by western blot analysis. | conventional mouse hybridoma technology was utilized to produce a panel of monoclonal antibodies which reacted with baculovirus proteins. using an enzyme-linked immunosorbent assay (elisa), the hybridomas which were raised against polyhedrin from autographa californica nuclear polyhedrosis virus (acnpv) and choristoeura fumiferana nuclear polyhedrosis virus (cfnpv) were found to cross-react differentially with polyhedrins and granulins from several species of baculoviruses. hybridoma antibodies ... | 1983 | 6340331 |
| baculovirus replication: inhibition of trichoplusia ni multiple nuclear polyhedrosis virus by [e]-5-(2-bromovinyl)-2'-deoxyuridine. | [e]-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) inhibits the replication of the baculovirus trichoplusia ni multiple nucleocapsid nuclear polyhedrosis virus in spodoptera frugiperda cells. virus-specific dna synthesis and late protein synthesis are suppressed by the drug. bvdu is phosphorylated by deoxythymidine (deoxycytidine) kinase present in both uninfected and virus-infected cells, and in its 5'-triphosphate form it inhibits dna polymerase activity in virus-infected cells. the effect of the bvd ... | 1983 | 6343553 |
| persistent baculovirus infections: spodoptera frugiperda npv and autographa californica npv in spodoptera frugiperda cells. | establishment of a persistent infection of spodoptera frugiperda nuclear polyhedrosis virus (npv) in spodoptera frugiperda (s.f.) cells occurred in three phases: the first phase was characterised by high levels of cell infection and death, the second phase by decreasing cell infection levels leading to the final phase where less than one per cent of the cells were infected during any subculture. the virus persisted at this level of infection provided the cells were maintained by regular subcultu ... | 1983 | 6360080 |
| baculovirus replication: phosphorylation of polypeptides synthesized in trichoplusia ni nuclear polyhedrosis virus-infected cells. | a number of polypeptides synthesized specifically in trichoplusia ni multiple nucleocapsid nuclear polyhedrosis virus (t. ni mnpv)-infected spodoptera frugiperda cells are phosphorylated both early and late in infection. certain non-structural proteins and the major basic internal protein are the main phosphoproteins detected in infected cells. the polyhedron protein was not phosphorylated. many cell proteins continue to be phosphorylated throughout infection. pulse-chase experiments have shown ... | 1984 | 6379101 |
| purification of a human alternative complement pathway inhibitor from hemolymph of larval fall armyworm (spodoptera frugiperda). | larval spodoptera frugiperda hemolymph contains a specific inhibitor of the alternative pathway of human complement. this inhibitor was purified from larval hemolymph (hl) by 50% (nh4)2so4 precipitation, deae-sephacel chromatography and sequential gel-filtration on bio-gel 1.5m, 0.5m and sephacryl s-200. purified hl protein (mr = 110,000) was composed of two mr 55,000 polypeptide chains. addition of purified hl protein to human complement resulted in a dose-dependent inhibition of rarbc lysis an ... | 1984 | 6477568 |
| bacillus thuringiensis var israelensis crystal delta-endotoxin: effects on insect and mammalian cells in vitro and in vivo. | bacillus thuringiensis var israelensis parasporal crystal delta-endotoxin was purified by ultracentrifugation on a discontinuous sucrose gradient. native delta-endotoxin crystals showed no detectable toxicity in the vitro and in vivo systems that are described. by contrast alkali-solubilized crystal delta-endotoxin caused rapid cytological and cytopathological changes in aedes albopictus, choristoneura fumiferana 63 cf1, spodoptera frugiperda and trichoplusia ni cell lines as observed by phase-c ... | 1983 | 6874728 |
| improved replication of autographa californica nuclear polyhedrosis virus in roller bottles: characterization of the progeny virus. | a reproducible growth curve was established for the propagation of autographa californica (speyer) nuclear polyhedrosis virus (acmnpv) in a continuous insect line from spodoptera frugiperda (j. e. smith) during large-volume production. a newly developed method for quantitation of polyhedra inclusion bodies (pib) by electron microscopy (em) during the growth cycle was compared to hemocytometer counts. the virus particles (vp) ratio to pfu and vp per pib were established by em methods. optimal yie ... | 1981 | 7028679 |
| [transfection conditions for baculovirus dna in an insect cell line]. | the following conditions were found to be necessary to achieve infection of a spodoptera frugiperda cell line with purified dna of nuclear polyhedrosis virus of spodoptera exempta (npv): (1) cell monolayers shall be prepared one day before the experiment; the number of cells added to 35-mm wells of plastic clusters (falcon) shall be 1.0 x10(6)-1.1x10(6), and the cells shall be in the stage of logarythmic growth; dna shall be diluted in hebs-buffer (ph 6.95-7.05); the volume of the mixtures added ... | 1982 | 7090350 |
| agonist-induced desensitization of dopamine d1 receptor-stimulated adenylyl cyclase activity is temporally and biochemically separated from d1 receptor internalization. | the regulation of the dopamine d1 receptor was investigated by using c-myc epitope-tagged d1 receptors expressed in sf9 (fall armyworm ovary) cells. treatment of d1 receptors with 10 microm dopamine for 15 min led to a loss of the dopamine-detected high-affinity state of the receptor accompanying a 40% reduction in the ability of the receptor to mediate maximal dopamine stimulation of adenylyl cyclase activity. after 60 min of agonist exposure, 45 min after the occurrence of desensitization, 28% ... | 1995 | 7479745 |
| immune protection conferred by the baculovirus-related glycoprotein of thogoto virus (orthomyxoviridae). | the coding region of segment 4 of thogoto (tho) virus, a tick-borne member of the orthomyxoviridae, was expressed in a baculovirus system under the control of the polyhedrin promoter. this construct expressed authentic envelope glycoprotein as determined by size and antigenic reactivity with a panel of monoclonal antibodies (mabs). immunization of hamsters with spodoptera frugiperda (sf21) cells infected with the recombinant baculovirus induced neutralizing and protective antibodies against viru ... | 1995 | 7483270 |
| high level production and localization of bovine rod cgmp-gated cation channel subunit 1 in baculovirus-infected insect cells and saccharomyces cerevisiae. | the cgmp-gated cation channel is responsible for the last step in the vertebrate phototransduction cascade which couples light activation of rhodopsin to a change in membrane permeability. two different expression systems, baculovirus-infected spodoptera frugiperda (sf9) insect cells and saccharomyces cerevisiae, were used for the overproduction of the cgmp-gated cation channel subunit 1 of bovine rod cells. presence of recombinant channel protein was monitored by sds-page and western-blot analy ... | 1995 | 7488067 |
| modulation of cd4 lateral mobility in intact cells by an intracellularly applied antibody. | this study shows that the lateral mobility of cd4, an important plasma-membrane immune receptor, can be modulated by intracellular application of an anti-cd4 antibody. for this purpose, (i) full-length cd4 and a truncated cd4 mutant, lacking a 32-residue-long c-terminal intracellularly exposed domain, were expressed in spodoptera frugiperda (sf9) insect cells, (ii) a monoclonal antibody, c6, with specificity for the c-terminal domain was generated, and (iii) a versatile apparatus for fluorescenc ... | 1995 | 7492321 |
| enzyme-linked immunosorbent assay using a recombinant baculovirus-expressed bacillus anthracis protective antigen (pa): measurement of human anti-pa antibodies. | we developed an antigen capture enzyme-linked immunosorbent assay (elisa) which does not require purified protective antigen (pa) for detection of human antibodies to bacillus anthracis pa. lysates of spodoptera frugiperda (sf-9) cells infected with recombinant baculovirus containing the pa gene were used as the source of pa to develop the elisa. recombinant pa from crude sf-9 cell lysates or pa purified from b. anthracis sterne strain was captured by an anti-pa monoclonal antibody coated onto m ... | 1994 | 7496927 |
| cloning and expression of a rat neuronal nitric oxide synthase coding sequence in a baculovirus/insect cell system. | a dna sequence encoding rat neuronal no synthase (nnos) was isolated and cloned into the baculovirus expression vector pvl1393 to generate pvlrbnos. transfection of spodoptera frugiperda sf-21 cells with the construct pvlrbnos resulted in the synthesis of high levels of neuronal no synthase. analysis of the expression pattern revealed soluble, enzymatically active no synthase in the cytoplasm of cell extracts. active enzyme could also be purified from culture supernatants using 2'-5' adp sepharo ... | 1993 | 7504478 |
| expression of infectious pancreatic necrosis virus polyprotein and vp1 in insect cells and the detection of the polyprotein in purified virus. | in order to study the molecular biology of infectious pancreatic necrosis virus (ipnv) replication, six different recombinant baculoviruses were constructed. the following four recombinants contained genome segment a-specific sequences; (i) acpp contained the complete polyprotein coding region and spodoptera frugiperda (sf) cells infected by these recombinants synthesized the 106-kda polyprotein (nh2-prevp2-ns protease-vp3-cooh), which was only partially processed by the protease to yield prevp2 ... | 1994 | 7507279 |
| expression, glycosylation, and phosphorylation of human keratins 8 and 18 in insect cells. | the filament forming ability and post-translational modifications of the human intermediate filaments, keratin polypeptides 8 and 18 (k8/18), were studied in recombinant baculovirus-infected insect (spodoptera frugiperda, sf9) cells. no change in cell morphology was noted after high levels of k8/18 were expressed in sf9 cells coinfected with recombinant virus-containing human k8 and k18. immunofluorescence staining showed that k8/18 expressed in sf9 cells formed somewhat what disorganized and ro ... | 1994 | 7510249 |
| respiratory syncytial virus nucleocapsid protein (n) expressed in insect cells forms nucleocapsid-like structures. | the gene coding for the n protein of rsv strain long has been cloned and sequenced. it was introduced behind the polyhedrin promoter of the shuttle vector pvl941 and baculoviruses containing the n gene were constructed by homologous recombination. infection of spodoptera frugiperda 9 cells resulted in the production of large amounts of a protein similar in size and antigenicity to the authentic n protein. the baculovirus expressed n protein was concentrated in the cytoplasm of the insect cells a ... | 1994 | 7513931 |
| desensitization, phosphorylation and palmitoylation of the human dopamine d1 receptor. | the regulation and post-translational modifications of the human dopamine d1 receptor were studied in the baculovirus-eukaryotic cell expression system. baculovirus constructs containing either the dna encoding the dopamine d1 receptor or a dna encoding a c-myc epitope tagged dopamine d1 receptor (c-myc-dopamine d1 receptor) were used to infect spodoptera frugiperda (sf9) insect cells. expressed dopamine d1 and c-myc-dopamine d1 receptors bound agonists and antagonists with affinities and a rank ... | 1994 | 7515822 |
| unprocessed foot-and-mouth disease virus capsid precursor displays discontinuous epitopes involved in viral neutralization. | a foot-and-mouth disease virus (fmdv) cdna cassette containing sequences encoding the capsid precursor p1, peptide 2a and a truncated 2b (abbreviated p1-2a) of type c fmdv, has been modified to generate the authentic amino terminus and the myristoylation signal. this construct has been used to produce a recombinant baculovirus (acmm53) which, upon infection of spodoptera frugiperda insect cells, expressed a recombinant p1-2a precursor with a high yield. this polyprotein reacted with neutralizing ... | 1994 | 7515974 |
| high-level expression of functional glutamate receptor channels in insect cells. | we have expressed glutamate-gated ion channels in spodoptera frugiperda sf21 insect cells using a recombinant baculovirus system. cells infected with recombinant baculoviruses encoding the alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (ampa)-selective glutamate receptor channel subunits glur-b and glur-d displayed specific high-affinity [3h]ampa binding (apparent dissociation constant kd of 15 nm for glur-b and 40 nm for glur-d) with pharmacological profiles typical of ampa receptors. the ... | 1994 | 7519022 |
| ligand-binding properties and n-glycosylation of alpha 1 subunit of the alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionate(ampa)-selective glutamate receptor channel expressed in a baculovirus system. | the alpha 1 subunit of the mouse alpha-amino-3-hydroxy-5-methyl-4-isoxazole- propionate(ampa)-selective glutamate receptor channel has been expressed in insect spodoptera frugiperda cells using a baculovirus system. the recombinant receptor proteins were identified by immunocytochemical detection, western-blot analysis, and [35s]methionine/[35s]cysteine metabolic labeling experiments. the effect of tunicamycin on the metabolic labeling and immunoblots suggested that the two products, a major pro ... | 1994 | 7519985 |
| characterization of the spodoptera frugiperda tata-binding protein: nucleotide sequence and response to baculovirus infection. | a cdna clone containing a 921 bp open-reading frame (307 amino acids; 34 kda) homologous to the tata-binding protein (tbp) was isolated and sequenced from a spodoptera frugiperda cell line that is commonly used in the baculovirus expression system. analysis of the s. frugiperda tbp (sftbp) sequence showed that the amino-terminal portion of sftbp diverged significantly from that of other tbp sequences including drosophila melanogaster whereas the carboxy-terminal sequence was highly conserved. so ... | 1994 | 7520800 |
| reconstitution of the influenza virus m2 ion channel in lipid bilayers. | m2, an integral membrane protein of influenza a virus, was purified from either influenza a virus-infected cv-1 cells or from spodoptera frugiperda (sf9) cells infected with a recombinant-m2 baculovirus. the purified protein, when incorporated into phospholipid bilayer membranes, produced ion-permeable channels with the following characteristics: (1) the channels appeared in bursts during which unit conductances of diverse magnitudes (25-500 ps) were observed. (2) the most probable open state wa ... | 1994 | 7535851 |
| two domains with amino-acid sequence similarity are required for dihydroneopterin aldolase function in the multifunctional folic acid synthesis fas protein of pneumocystis carinii. | the folic acid synthesized gene (fas) of pneumocystis carinii (pc) codes for a multifunctional enzyme (fas) known to catalyse three consecutive steps leading to the production of dihydropteroate in the de novo folate synthesis pathway. previously, we predicted that a domain, designated fasb (amino acids (aa) 161-280), of the 740-aa multifunctional protein contains the first of the three enzyme activities in the pathway, namely dihydroneopterin aldolase (dhna), since it shares 23% aa identity wit ... | 1995 | 7543066 |
| cloning and expression of a cdna encoding bovine retinal pigment epithelial 11-cis retinol dehydrogenase. | identification of a 32-kd protein in the bovine retinal pigment epithelium. | 1995 | 7544779 |
| stable expression of maize auxin-binding protein in insect cell lines. | to achieve continuous expression of the major maize auxin-binding protein (abp1) in insect cells, the abp1 gene coding region was placed under control of a baculovirus immediate-early gene promoter and transfected into spodoptera frugiperda sf9 cells. the abp1 gene was detected in twelve cell lines, one of which was selected for detailed analysis. immunolocalisation demonstrated that abp1 was targeted to and retained in the endoplasmic reticulum (er), in accordance with its signal peptide and ca ... | 1995 | 7556613 |
| biochemical analysis of the n-glycosylation pathway in baculovirus-infected lepidopteran insect cells. | the baculovirus-insect cell system is used routinely for foreign glycoprotein production, but the precise nature of the n-glycosylation pathway in this system remains unclear. some studies indicate that these cells cannot process n-linked oligosaccharides to complex forms containing outer-chain galactose and sialic acid, while others indicate that they can. in this study, we used the major virion envelope glycoprotein of the baculovirus autographa california multicapsid nuclear polyhedrosis viru ... | 1995 | 7571420 |
| cdna-directed expression of human cytochrome p450 cyp1a1 using baculovirus. purification, dependency on nadph-p450 oxidoreductase, and reconstitution of catalytic properties without purification. | a recombinant baculovirus containing the human cytochrome p450 (cyp) 1a1 cdna was constructed and used to express cyp1a1 in spodoptera frugiperda (sf9) insect cells (0.14 +/- 0.04 nmol/mg protein, 53 +/- 14 nmol/liter, n = 30). the enzyme represented approximately 1% of total cellular protein and was partially purified by a three-column procedure to a specific content of 5.0 nmol/mg protein. catalytic activity was reconstituted with both the purified enzyme using lipid and nadph-p450 oxidoreduct ... | 1995 | 7587956 |
| horseradish peroxidase phe172-->tyr mutant. sequential formation of compound i with a porphyrin radical cation and a protein radical. | a gene coding for the f172y mutant of horseradish peroxidase isozyme c (hrp) has been constructed and expressed in both spodoptera frugiperda (sf-9) and trichoplusia ni egg cell homogenate (highfive) cells. homology modeling with respect to three peroxidases for which crystal structures are available places phe172 on the proximal side of the heme in the vicinity of porphyrin pyrrole ring c. the ph optimum and spectroscopic properties of the f172y mutant are essentially identical to those of wild ... | 1995 | 7629167 |
| expression of oligohistidine-tagged ricin b chain in spodoptera frugiperda. | dna encoding adpgh6g was fused to the 5'-end of rtb dna and subcloned as a bamhi-ecori dna cassette into the baculovirus transfer vector, pacgp67a. spodoptera frugiperda sf9 cells were cotransfected with pacgp67a-adpgh6g-rtb dna and baculogold acnpv dna, and recombinant baculovirus was isolated by two cycles of limiting dilution assay followed by dot blot analysis with 32p-dctp random primer labeled rtb dna. recombinant virus was purified and amplified to obtain stocks at titers of 10(7) infecti ... | 1994 | 7533004 |
| expression of pe38 and ie2, viral members of the c3hc4 finger family, during baculovirus infection: pe38 and ie2 localize to distinct nuclear regions. | the pe38 gene of autographa californica nuclear polyhedrosis virus represents one of the major early transcripts after viral infection. the function of the pe38 protein, which contains a c3hc4 zinc finger motif, is still not understood. we have raised polyclonal antiserum against the pe38 protein, pe38, produced in bacteria to investigate pe38 expression in the course of infection. a approximately 38-kda polypeptide is first detectable at 2 h postinfection and decreases rapidly after 24 h. durin ... | 1995 | 7636971 |
| the rotavirus nonstructural glycoprotein nsp4 mobilizes ca2+ from the endoplasmic reticulum. | we previously reported that expression of rotavirus nonstructural glycoprotein nsp4 is responsible for an increase in cytosolic free ca2+ concentration ([ca2+]i) in spodoptera frugiperda (sf9) insect cells (p. tian, y. hu, w. p. schilling, d. a. lindsay, j. eiden, and m. k. estes, j. virol. 68:251-257, 1994). the purpose of the present study was to determine the mechanism by which nsp4 causes an increase in [ca2+]i by measuring the permeability of the cytoplasmic and endoplasmic reticulum (er) m ... | 1995 | 7637021 |
| functional reconstitution in lipid vesicles of influenza virus m2 protein expressed by baculovirus: evidence for proton transfer activity. | the influenza virus m2 protein was expressed from a recombinant baculovirus in spodoptera frugiperda sf9 cells, purified and reconstituted into artificial membrane vesicles. the specific inhibitor amantadine overcame the toxic activity of the protein and boosted the rate of m2 synthesis by a factor of 10, allowing yields of about 1 mg of purified m2 protein per g of sf9 cells. m2 protein expressed in this system was phosphorylated and palmitoylated and displayed properties similar to the authent ... | 1994 | 7527837 |
| characterization of neuronal nitric oxide synthase and a c415h mutant, purified from a baculovirus overexpression system. | nitric oxide synthase (nos) catalyzes the conversion of l-arginine to citrulline and nitric oxide (.no). a baculovirus overexpression system has been developed for a constitutive nos isoform, cloned originally from rat cerebellum (b-nos). recombinant virus was used at a multiplicity of infection of 5 to infect spodoptera frugiperda cells in culture, and nos was expressed to 10% of the total soluble protein at 48 h postinfection. in order to express catalytically active enzyme, it was necessary t ... | 1994 | 7527656 |
| comparison of the non-structural protein, ns1, of tick-borne and insect-borne orbiviruses. | the nucleotide sequence of rna segment 6 of broadhaven virus (brdv), a tick-borne orbivirus, was determined principally from two overlapping cdna clones and rna end sequence analysis. the genome segment is 1714 base pairs in length and has a coding capacity for a protein of 537 amino acids, having a net charge of +4.0 at neutral ph. comparison of the predicted amino acid sequence of brdv rna segment 6 with the ns1 sequence of insect-borne orbiviruses, bluetongue virus (btv), african horse sickne ... | 1995 | 7653106 |
| fkbp46, a novel sf9 insect cell nuclear immunophilin that forms a protein-kinase complex. | recently, we identified a 59-kda nuclear phosphoprotein that is associated with a recombinant mouse fkbp-52 (alnemri, e. s., fernandes-alnemri, t., nelki, d. s., dudley, k., dubois, g. c., and litwack, g. (1993) proc. natl. acad. sci. u.s.a. 90, 6839-6843). here we describe the cloning, overexpression, and characterization of this protein from spodoptera frugiperda insect cells (sf9 cells). the cloned cdna codes for an acidic protein of 412 amino acids with distinct structural domains. starting ... | 1994 | 7527037 |
| efficient complementary dna directed expression of human fetal liver cytochrome p450 (cyp3a7) in insect cells using baculovirus. | cyp3a7 is a form of cytochrome p450, which is expressed specifically in human fetal livers. npvhf1, a recombinant baculovirus containing the entire coding region of cyp3a7, was constructed and infected to spodoptera frugiperda (sf9) cells. upon infection with npvhf1, the sf9 cells expressed the cyp3a7 to the maximum content of 0.2 nmol per mg of whole cell lysates 72 hours after infection. a 5.5-fold expression level (1.1 nmol per mg of whole cell lysates) was attainable when cultured in the pre ... | 1995 | 7663401 |
| baculovirus-driven expression and purification of glycine receptor alpha 1 homo-oligomers. | the glycine receptor is a ligand-gated anion channel protein of postsynaptic membranes. we expressed a homo-oligomeric receptor composed of human alpha 1 subunits in spodoptera frugiperda cells by infection with a recombinant autographa californica nuclear polyhedrosis virus. a substantial fraction of the recombinant receptor was incorporated as a functional channel protein into the cell's plasma membrane at expression levels 4- to 30-fold higher than in other eukaryotic heterologous expression ... | 1995 | 7635206 |
| comparison of the enzymatic properties of the na,k-atpase alpha 3 beta 1 and alpha 3 beta 2 isozymes. | the coexpression of multiple isoforms of the alpha and beta subunits of the na,k-atpase in mammalian tissues gives rise to the complex molecular heterogeneity that characterizes the na pump. the expression of the different na,k-atpase isoforms in insect cells using recombinant baculoviruses represents a useful system for the analysis of na,k-atpase isoform function. in the present study, we use this system to direct the expression of the rat na,k-atpase alpha 3 beta 1 and alpha 3 beta 2 in sf-9 ... | 1995 | 7632689 |