| only a minority of broad-range detoxification genes respond to a variety of phytotoxins in generalist bemisia tabaci species. | generalist insect can utilize two different modes for regulating their detoxification genes, the constitutive mode and the induced mode. here, we used the bemisia tabaci sibling species meam1 and med, as a model system for studying constitutive and induced detoxification resistance and their associated tradeoffs. b. tabaci adults were allowed to feed through membranes for 24 h on diet containing only sucrose or sucrose with various phytotoxins. quantitative real-time pcr analyses of 18 detoxific ... | 2015 | 26655836 |
| characterization and comparative profiling of mirnas in invasive bemisia tabaci (gennadius) b and q. | micrornas (mirnas) are small, conserved, non-coding rnas that post-transcriptionally regulate gene expression. bemisia tabaci (gennadius) b and q are two invasive and dominant whiteflies, and b. tabaci q has been displacing b in china. differences in biological traits (fecundity, host range, resistance to insecticides, etc.) as affected by mirnas might be involved in the displacement. in this study, we performed high-throughput sequencing to identify mirnas in b. tabaci b and q. | 2013 | 23527280 |
| transcriptomic analysis of the salivary glands of an invasive whitefly. | some species of the whitefly bemisia tabaci complex cause tremendous losses to crops worldwide through feeding directly and virus transmission indirectly. the primary salivary glands of whiteflies are critical for their feeding and virus transmission. however, partly due to their tiny size, research on whitefly salivary glands is limited and our knowledge on these glands is scarce. | 2012 | 22745728 |
| gene expression profiling in the thiamethoxam resistant and susceptible b-biotype sweetpotato whitefly, bemisia tabaci. | thiamethoxam has been used as a major insecticide to control the b-biotype sweetpotato whitefly, bemisia tabaci (gennadius) (hemiptera: aleyrodidae). due to its excessive use, a high level of resistance to thiamethoxam has developed worldwide over the past several years. to better understand the molecular mechanisms underlying this resistance in b. tabaci, gene profiles between the thiamethoxam-resistant and thiamethoxam-susceptible strains were investigated using the suppression subtractive hyb ... | 0 | 22957505 |
| influence of additives on the yield and pathogenicity of conidia produced by solid state cultivation of an isaria javanica isolate. | recently, the q biotype of tobacco whitefly has been recognized as the most hazardous strain of bemisia tabaci worldwide, because of its increased resistance to some insecticide groups. as an alternative control agent, we selected an isaria javanica isolate as a candidate for the development of a mycopesticide against the q biotype of sweet potato whitefly. to select optimal mass production media for solid-state fermentation, we compared the production yield and virulence of conidia between 2 su ... | 2014 | 25606006 |
| transcriptome profiling of the whitefly bemisia tabaci reveals stage-specific gene expression signatures for thiamethoxam resistance. | bemisia tabaci has developed high levels of resistance to many insecticides including the neonicotinoids and there is strong evidence that for some compounds resistance is stage-specific. to investigate the molecular basis of b. tabaci resistance to the neonicotinoid thiamethoxam we used a custom whitefly microarray to compare gene expression in the egg, nymph and adult stages of a thiamethoxam-resistant strain (th-r) with a susceptible strain (th-s). gene ontology and bioinformatic analyses rev ... | 2013 | 23889345 |
| inactivation of wolbachia reveals its biological roles in whitefly host. | the whitefly bemisia tabaci is cryptic species complex composed of numerous species. individual species from the complex harbor a diversity of bacterial endosymbionts including wolbachia. however, while wolbachia is known to have a number of different roles, its role in b. tabaci is unclear. here, the antibiotic rifampicin is used to selectively eliminate wolbachia from b. tabaci so as to enable its roles in whitefly development and reproduction to be explored. the indirect effects of wolbachia ... | 2012 | 23144739 |
| tomato yellow leaf curl virus: no evidence for replication in the insect vector bemisia tabaci. | begomovirus ssdna plant virus (family geminiviridae) replication within the bemisia tabaci vector is controversial. transovarial transmission, alteration to whitefly biology, or detection of viral transcripts in the vector are proposed as indirect evidence of replication of tomato yellow leaf curl virus (tylcv). recently, contrasting direct evidence has been reported regarding the capacity of tylcv to replicate within individuals of b. tabaci based on quantitave pcr approaches. time-course exper ... | 2016 | 27476582 |
| cloning and functional characterization of c-jun nh2-terminal kinase from the mediterranean species of the whitefly bemisia tabaci complex. | c-jun nh2-terminal kinase (jnk) signaling is a highly conserved pathway that controls gene transcription in response to a wide variety of biological and environmental stresses. in this study, a jnk from the invasive mediterranean (med) species of the whitefly bemisia tabaci complex was cloned and characterized. the full-length jnk cdna of med consists of 1565 bp, with an 1176 bp open reading frame encoding 392 amino acids. comparison of jnk amino acid sequences among different species showed tha ... | 2013 | 23807503 |
| estimation of the whitefly bemisia tabaci genome size based on k-mer and flow cytometric analyses. | whiteflies of the bemisia tabaci (hemiptera: aleyrodidae) cryptic species complex are among the most important agricultural insect pests in the world. these phloem-feeding insects can colonize over 1000 species of plants worldwide and inflict severe economic losses to crops, mainly through the transmission of pathogenic viruses. surprisingly, there is very little genomic information about whiteflies. as a starting point to genome sequencing, we report a new estimation of the genome size of the b ... | 2015 | 26463411 |
| flow cytometry and k-mer analysis estimates of the genome sizes of bemisia tabaci b and q (hemiptera: aleyrodidae). | the genome sizes of the b- and q-types of the whitefly bemisia tabaci (gennnadius) were estimated using flow cytometry (drosophila melanogaster as the dna reference standard and propidium iodide (pi) as the fluorochrome) and k-mer analysis. for flow cytometry, the mean nuclear dna content was 0.686 pg for b-type males, 1.392 pg for b-type females, 0.680 pg for q-type males, and 1.306 pg for q-type females. based on the relationship between dna content and genome size (1 pg dna = 980 mbp), the ha ... | 2015 | 26042041 |
| the draft genome of whitefly bemisia tabaci meam1, a global crop pest, provides novel insights into virus transmission, host adaptation, and insecticide resistance. | the whitefly bemisia tabaci (hemiptera: aleyrodidae) is among the 100 worst invasive species in the world. as one of the most important crop pests and virus vectors, b. tabaci causes substantial crop losses and poses a serious threat to global food security. | 2016 | 27974049 |
| effects of host plant factors on the bacterial communities associated with two whitefly sibling species. | although discrepancy in the specific traits and ecological characteristics of bemisia tabaci between species are partially attributed to the b. tabaci-associated bacteria, the factors that affect the diversity of b. tabaci-associated bacteria are not well-understood. we used the metagenomic approach to characterize the b. tabaci-associated bacterial community because the approach is an effective tool to identify the bacteria. | 2016 | 27008327 |
| transcriptomic dissection of sexual differences in bemisia tabaci, an invasive agricultural pest worldwide. | sex difference involving chromosomes and gene expression has been extensively documented. in this study, the gender difference in the sweetpotato whitefly bemisia tabaci was investigated using illumina-based transcriptomic analysis. gender-based rnaseq data produced 27 gb reads, and subsequent de novo assembly generated 93,948 transcripts with a n50 of 1,853 bp. a total of 1,351 differentially expressed genes were identified between male and female b. tabaci, and majority of them were female-bia ... | 2014 | 24526031 |
| implication of the whitefly bemisia tabaci cyclophilin b protein in the transmission of tomato yellow leaf curl virus. | tomato yellow leaf curl virus (tylcv) is a single-stranded (ssdna) begomoviruses that causes severe damage to tomato and several other crops worldwide. tylcv is exclusively transmitted by the sweetpotato whitefly, bemisia tabaci in a persistent circulative and propagative manner. previous studies have shown that the transmission, retention, and circulation of tylcv in its vector involves interaction with insect and endosymbiont proteins, which aid in the transmission of the virus, or have a prot ... | 2016 | 27895657 |
| deciphering the biology of deltasatellites from the new world: maintenance by new world begomoviruses and whitefly transmission. | deltasatellites are small noncoding dna satellites associated with begomoviruses. the study presented here has investigated the biology of two deltasatellites found in wild malvaceous plants in the new world (nw). infectious clones of two nw deltasatellites (from malvastrum coromandelianum and sidastrum micranthum) and associated begomoviruses were constructed. infectivity in nicotiana benthamiana and their natural malvaceous hosts was assessed. the nw deltasatellites were not able to spread aut ... | 2016 | 27400152 |
| molecular identification of ageratum enation virus, betasatellite and alphasatellite molecules isolated from yellow vein diseased amaranthus cruentus in india. | natural occurrence of yellow vein disease on amaranthus cruentus was observed at lucknow, india in the year 2008. the causal virus was successfully transmitted through whiteflies (bemisia tabaci) from diseased a. cruentus to healthy seedlings of a. cruentus and other test species which indicated begomovirus infection. the begomovirus dna-a, betasatellite, and alphasatellite components associated with yellow vein disease were amplified by rolling circle amplification using ø-29 dna polymerase fro ... | 2013 | 23963765 |
| agroinoculation of the cloned infectious cdnas of lettuce chlorosis virus results in systemic plant infection and production of whitefly transmissible virions. | lettuce chlorosis virus (lcv) is a single stranded, positive strand rna virus that is solely transmitted by specific whitefly vectors (bemisia tabaci biotypes a and b) but not by mechanical leaf-rub inoculation. the roles of viral encoded proteins involved in the infection cycle of lcv have not yet been characterized due to the lack of reverse genetic tools. we present here a report of the successful development of an agrobacterium-mediated inoculation system for the cloned cdna constructs of lc ... | 2012 | 22926259 |
| isolation and identification of culturable bacteria from honeydew of whitefly, bemisia tabaci (g.) (hemiptera: aleyrodidae). | bemisia tabaci (g.) is an important pest and a vector of gemini viruses infecting plants. during the process of feeding b. tabaci excretes honeydew which is rich in nutrients, and an excellent medium for microbial growth. recent report proved that volatile emitted by the honeydew associated bacteria of aphid, acyrthosiphon pisum harris was involved in natural enemy calling. thus understanding the honeydew associated bacteria is of paramount importance from the non-chemical method of insect pest ... | 2014 | 25606395 |
| transfer of cry1ac and cry2a proteins from genetically engineered bt cotton to herbivores and predators. | with the cultivation of bt cotton, the produced insecticidal cry proteins are ingested by herbivores and potentially transferred along the food chain to natural enemies, such as predators. in laboratory experiments with bollgard ii cotton, concentrations of cry1ac and cry2ab were measured in lepidoptera larvae (spodoptera littoralis, heliothis virescens), plant bugs (euschistus heros), aphids (aphis gossypii), whiteflies (bemisia tabaci), thrips (thrips tabaci, frankliniella occidentalis), and s ... | 2017 | 28374515 |
| large-scale, spatially-explicit test of the refuge strategy for delaying insecticide resistance. | the refuge strategy is used worldwide to delay the evolution of pest resistance to insecticides that are either sprayed or produced by transgenic bacillus thuringiensis (bt) crops. this strategy is based on the idea that refuges of host plants where pests are not exposed to an insecticide promote survival of susceptible pests. despite widespread adoption of this approach, large-scale tests of the refuge strategy have been problematic. here we tested the refuge strategy with 8 y of data on refuge ... | 2012 | 22215605 |
| reduction of viral load in whitefly (bemisia tabaci gen.) feeding on rnai-mediated bean golden mosaic virus resistant transgenic bean plants. | the rnai concept was explored to silence the rep gene from the bean golden mosaic virus (bgmv) and a genetically modified (gm) bean immune to the virus was previously generated. we investigated if bgmv-viruliferous whiteflies would reduce viral amount after feeding on gm plants. bgmv dna amount was significantly reduced in whiteflies feeding in gm-plants (compared with insects feeding on non-gm plants) for a period of 4 and 8 days in 52% and 84% respectively. | 2015 | 26297125 |
| high ozone (o3) affects the fitness associated with the microbial composition and abundance of q biotype bemisia tabaci. | ozone (o3) affects the fitness of an insect, such as its development, reproduction and protection against fungal pathogens, but the mechanism by which it does so remains unclear. here, we compared the fitness (i.e., the growth and development time, reproduction and protection against beauveria bassiana (b. bassiana) of q biotype whiteflies fumigated under ho3 (280 ± 20 ppb) and control o3 (50 ± 10 ppb) concentrations. moreover, we determined that gene expression was related to development, repro ... | 2016 | 27799921 |
| survey of indigenous entomopathogenic fungi and evaluation of their pathogenicity against the carmine spider mite, tetranychus cinnabarinus (boisd.), and the whitefly, bemisia tabaci (genn.) biotype b. | the carmine spider mite, tetranychus cinnabarinus, and the silverleaf whitefly, bemisia tabaci, are serious pests of both field- and greenhouse-grown crops in south-western turkey. control of these pests has been heavily dependent upon chemical pesticides. the objectives of this study were to investigate the occurrence of indigenous entomopathogenic fungi (epf) in field populations of t. cinnabarinus and b. tabaci, and to evaluate their pathogenicity against these pests. for this purpose, a surv ... | 2016 | 26929052 |
| how to start with a clean crop: biopesticide dips reduce populations of bemisia tabaci (hemiptera: aleyrodidae) on greenhouse poinsettia propagative cuttings. | (1) global movement of propagative plant material is a major pathway for introduction of bemisia tabaci (hemiptera: aleyrodidae) into poinsettia greenhouses. starting a poinsettia crop with high pest numbers disrupts otherwise successful biological control programs and widespread resistance of b. tabaci against pesticides is limiting growers' options to control this pest; (2) this study investigated the use of several biopesticides (mineral oil, insecticidal soap, beauveria bassiana, isaria fumo ... | 2016 | 27681741 |
| improved shelf life of dried beauveria bassiana blastospores using convective drying and active packaging processes. | the yeast form (blastospore) of the dimorphic insect-pathogenic fungus beauveria bassiana can be rapidly produced using liquid fermentation methods but is generally unable to survive rapid dehydration processes or storage under non-refrigerated conditions. in this study, we evaluated the influence of two convective drying methods, various modified atmosphere packaging systems, and storage temperatures on the desiccation tolerance, storage stability, and virulence of blastospores of b. bassiana e ... | 2016 | 27198727 |
| tri-tek (petroleum horticultural oil) and beauveria bassiana: use in eradication strategies for bemisia tabaci mediterranean species in uk glasshouses. | the sweetpotato whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae) is a pest of global importance on both outdoor and glasshouse crops. to date, b. tabaci has not become established in the uk. the uk holds protected zone status against this pest and, as a result, b. tabaci entering on plant material is subjected to a policy of eradication. mediterranean species is now the most prevalent bemisia species entering the uk. increasing neonicotinoid resistance is becoming increasingly widesp ... | 2015 | 26463071 |
| glucose concentration alters dissolved oxygen levels in liquid cultures of beauveria bassiana and affects formation and bioefficacy of blastospores. | the filamentous fungus beauveria bassiana is an economically important pathogen of numerous arthropod pests and is able to grow in submerged culture as filaments (mycelia) or as budding yeast-like blastospores. in this study, we evaluated the effect of dissolved oxygen and high glucose concentrations on blastospore production by submerged cultures of two isolates of b. bassiana, esalq1432 and gha. results showed that maintaining adequate dissolved oxygen levels coupled with high glucose concentr ... | 2015 | 25947245 |
| liquid culture fermentation for rapid production of desiccation tolerant blastospores of beauveria bassiana and isaria fumosorosea strains. | a major constraint to the commercial use of fungal biocontrol agents is the availability of low-cost production media and processes. previous attempts in producing beauveria blastospores using liquid culture fermentation processes required long fermentation times (6-8days) and produced cells that had poor survival after desiccation and storage. in this study, isolates of beauveria bassiana and isaria fumosorosea were evaluated for blastospore yield, desiccation tolerance, storage stability, and ... | 2015 | 25497914 |
| update on the status of bemisia tabaci in the uk and the use of entomopathogenic fungi within eradication programmes. | the sweetpotato whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae) continues to be a serious threat to crops worldwide. the uk holds protected zone status against this pest and, as a result, b. tabaci entering on plant material is subjected to a policy of eradication. both b and q bemisia biotypes are now regularly intercepted entering the uk. with increasing reports of neonicotinoid resistance in both these biotypes, it is becoming more problematic to control/eradicate. therefore, alt ... | 2013 | 26464385 |
| comparing the regional epidemiology of the cassava mosaic and cassava brown streak virus pandemics in africa. | the rapid geographical expansion of the cassava mosaic disease (cmd) pandemic, caused by cassava mosaic geminiviruses, has devastated cassava crops in 12 countries of east and central africa since the late 1980s. region-level surveys have revealed a continuing pattern of annual spread westward and southward along a contiguous 'front'. more recently, outbreaks of cassava brown streak disease (cbsd) were reported from uganda and other parts of east africa that had been hitherto unaffected by the d ... | 2011 | 21549776 |
| infectious clones of the crinivirus cucurbit chlorotic yellows virus are competent for plant systemic infection and vector transmission. | cucurbit chlorotic yellows virus (ccyv), a recently identified bipartite crinivirus, causes economic losses in cucurbit plants. ccyv is naturally transmitted only by whitefly bemisia tabaci. here we constructed full-length cdna clones of ccyv (rna1 and rna2) fused to the t7 rna polymerase promoter and the cauliflower mosaic virus 35s promoter. ccyv replicated and accumulated efficiently in cucumis sativus protoplasts transfected with in vitro transcripts. without rna2, rna1 replicated efficientl ... | 2016 | 26982585 |
| characterization of a new begomovirus and a beta satellite associated with the leaf curl disease of french bean in northern india. | begomoviruses are emerging as serious threat to many crops throughout the world particularly in tropical and sub-tropical regions. a leaf curl disease with symptoms typical of infection by many begomoviruses was observed in french bean (phaseolus vulgaris) at kanpur, india, during 2010-2012. the disease caused downward leaf curling and made the plants unproductive. the disease was transmitted from infected to healthy plants through whitefly (bemisia tabaci). the products of five samples digested ... | 2013 | 23054434 |
| rna viral metagenome of whiteflies leads to the discovery and characterization of a whitefly-transmitted carlavirus in north america. | whiteflies from the bemisia tabaci species complex have the ability to transmit a large number of plant viruses and are some of the most detrimental pests in agriculture. although whiteflies are known to transmit both dna and rna viruses, most of the diversity has been recorded for the former, specifically for the begomovirus genus. this study investigated the total diversity of dna and rna viruses found in whiteflies collected from a single site in florida to evaluate if there are additional, p ... | 2014 | 24466220 |
| host range and genetic diversity of croton yellow vein mosaic virus, a weed-infecting monopartite begomovirus causing leaf curl disease in tomato. | croton yellow vein mosaic virus (cyvmv) is a widely occurring begomovirus in croton bonplandianum, a common weed in the indian subcontinent. in this study, cyvmv (genus begomovirus, family geminiviridae) was transmitted by whiteflies (bemisia tabaci) to as many as 35 plant species belonging to 11 families, including many vegetables, tobacco varieties, ornamentals and weeds. cyvmv produced bright yellow vein symptoms in croton, whereas in all the other host species, the virus produced leaf curl s ... | 2013 | 23096697 |
| control of insect vectors and plant viruses in protected crops by novel pyrethroid-treated nets. | long-lasting insecticide-treated nets (llitns) constitute a novel alternative that combines physical and chemical tactics to prevent insect access and the spread of insect-transmitted plant viruses in protected enclosures. this approach is based on a slow-release insecticide-treated net with large hole sizes that allow improved ventilation of greenhouses. the efficacy of a wide range of llitns was tested under laboratory conditions against myzus persicae, aphis gossypii and bemisia tabaci. two n ... | 2015 | 25404196 |
| pepino mosaic virus and tomato torrado virus: two emerging viruses affecting tomato crops in the mediterranean basin. | the molecular biology, epidemiology, and evolutionary dynamics of pepino mosaic virus (pepmv) are much better understood than those of tomato torrado virus (totv). the earliest descriptions of pepmv suggest a recent jump from nontomato species (e.g., pepino; solanum muricatum) to tomato (solanum lycopersicum). its stability in contaminated plant tissues, its transmission through seeds, and the global trade of tomato seeds and fruits may have facilitated the global spread of pepmv. stability and ... | 2012 | 22682177 |
| field evaluation of cucurbit yellow stunting disorder virus transmission by bemisia tabaci. | cucurbit yellow stunting disorder virus (cysdv) is a whitefly-transmitted crinivirus (closteroviridae) that impacts melon production in many parts of the world including the usa. it has been responsible for melon crop loss in the southwestern u.s. since 2006 when it was first identified. control strategies have revolved mainly around chemical control, but research to identify suitable products and approaches to implementing them has lagged. the current study investigated the performance of four ... | 2017 | 28342998 |
| effects of foliar and systemic insecticides on whitefly transmission and incidence of cucurbit yellow stunting disorder virus. | cucurbit yellow stunting disorder virus (cysdv) is a cosmopolitan viral disease transmitted by bemisia tabaci that infects cucurbit crops. cantaloupe production in the southwestern usa has been confronted by epidemics of cysdv since 2006 when it was first identified in arizona and california. as a phloem-limited virus that is vectored in a semi-persistent manner by b. tabaci, cysdv has transmission characteristics that may be suppressed by select insecticide applications. | 2016 | 27862891 |
| assessing transmission of crop diseases by insect vectors in a landscape context. | theory indicates that landscape composition affects transmission of vector-borne crop diseases, but few empirical studies have investigated how landscape composition affects plant disease epidemiology. since 2006, bemisia tabaci (gennadius) has vectored the cucurbit yellow stunting disorder virus (cysdv) to cantaloupe and honeydew melons (cucumis melo l.) in the southwestern united states and northern mexico, causing significant reductions in yield of fall melons and increased use of insecticide ... | 2014 | 24665679 |
| a plant virus manipulates the behavior of its whitefly vector to enhance its transmission efficiency and spread. | plant viruses can produce direct and plant-mediated indirect effects on their insect vectors, modifying their life cycle, fitness and behavior. viruses may benefit from such changes leading to enhanced transmission efficiency and spread. in our study, female adults of bemisia tabaci were subjected to an acquisition access period of 72 h in tomato yellow leaf curl virus (tylcv)-infected and non-infected tomato plants to obtain viruliferous and non-viruliferous whiteflies, respectively. insects th ... | 2013 | 23613872 |
| identification and characterization of two phospholipid hydroperoxide glutathione peroxidase genes from the mediterranean species of the whitefly bemisia tabaci complex. | phospholipid hydroperoxide glutathione peroxidases (phgpxs) are essential enzymes of the cellular antioxidant defense system during insect-plant interactions. however, little attention has been devoted to the functional characterization of phgxps in the whitefly bemisia tabaci. here, we report the identification and characterization of two phgpx genes, designated as btq-phgpx1 and btq-phgpx2 from the mediterranean species of the b. tabaci complex. sequence analysis indicated that the length of b ... | 2015 | 25639712 |
| identification, characterization and expression of a defensin-like antifungal peptide from the whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae). | defensins are a class of small and diverse cysteine-rich proteins which have broad-spectrum antimicrobial activities. we identified and characterized a full-length cdna encoding a putative defensin-like peptide from the whitefly bemisia tabaci by race and quantitative real-time (qrt)-pcr. the full-length cdna, named btdef, was 388 bp long and contained an open reading frame of 228 bp. the putative mature btdef had 46 amino acids with a molecular weight of 5.06 kda. the deduced amino acid sequenc ... | 2013 | 23496414 |
| cloning, expression and characterization of mitochondrial manganese superoxide dismutase from the whitefly, bemisia tabaci. | a mitochondrial manganese superoxide dismutase from an invasive species of the whitefly bemisia tabaci complex (bt-mmnsod) was cloned and analyzed. the full length cdna of bt-mmnsod is 1210 bp with a 675 bp open reading frame, corresponding to 224 amino acids, which include 25 residues of the mitochondrial targeting sequence. compared with various vertebrate and invertebrate animals, the mnsod signature (dvwehayy) and four conserved amino acids for manganese binding (h54, h102, d186 and h190) we ... | 2013 | 23296268 |
| molecular studies on the transmission of indian cassava mosaic virus (icmv) and sri lankan cassava mosaic virus (slcmv) in cassava by bemisia tabaci and cloning of icmv and slcmv replicase gene from cassava. | cassava mosaic disease, caused by cassava mosaic geminiviruses are transmitted by bemisia tabaci. the b. tabaci adults from colonies reared on virus free cassava plant produced from apical meristem culture was studied to determine their ability to transmit indian cassava mosaic virus (icmv) and sri lankan cassava mosaic virus (slcmv) from cassava to cassava. virus free plants were confirmed by polymerase chain reaction (pcr) using geminivirus degenerate primers. the virus acquisition access peri ... | 2012 | 22302313 |
| lack of resistance development in bemisia tabaci to isaria fumosorosea after multiple generations of selection. | the emergence of insecticide resistant insect pests is of significant concern worldwide. the whitefly, bemisia tabaci, is an important agricultural pest and has shown incredible resilience developing resistance to a number of chemical pesticides. entomopathogenic fungi such as isaria fumosorosea offer an attractive alternative to chemical pesticides for insect control, and this fungus has been shown to be an effective pathogen of b. tabaci. little is known concerning the potential for the develo ... | 2017 | 28230074 |
| effects of isaria fumosorosea on tylcv (tomato yellow leaf curl virus) accumulation and transmitting capacity of bemisia tabaci. | tomato yellow leaf curl virus (tylcv) is transmitted by the bemisia tabaci pest middle east-asia minor 1 (meam1) in china. isaria fumosorosea is a fungal pathogen of b. tabaci. however, the effects of fungal infection on tylcv expression and transmission by meam1 are unclear. in this study, potted tomatoes containing second instar nymphs of meam1 were treated with i. fumosorosea ifb01 strain and the relationship between fungal infection in meam1 and its tylcv transmission capacity was investigat ... | 2016 | 27716852 |
| recombinant fungal entomopathogen rnai target insect gene. | rna interference (rnai) technology is considered as an alternative for control of pests. however, rnai has not been used in field conditions yet, since delivering exogenous ds/sirna to target pests is very difficult. the laboratory methods of introducing the ds/sirna into insects through feeding, micro feeding / dripping and injecting cannot be used in fields. transgenic crop is perhaps the most effective application of rnai for pest control, but it needs long-time basic researches in order to r ... | 2016 | 27715447 |
| biosafety of an entomopathogenic fungus isaria fumosorosea in an acute dermal test in rabbits. | isaria fumosorosea (formerly paecilomyces fumosoroseus) is an entomopathogenic fungus that has been proposed as a low risk environmental alternative to the use of chemical insecticides to control agricultural pests and disease vectors. although there are some mycoinsecticides already being marketed in many countries, not all their biosafety protocols have been published. the acute dermal toxicity test in an animal model is one in a series of biosafety protocols that must be developed, in order t ... | 2017 | 27055483 |
| pathogenicity of isaria fumosorosea to bemisia tabaci, with some observations on the fungal infection process and host immune response. | isaria fumosorosea is an important pathogen of whiteflies, and is used as a mycoinsecticide against this pest in many regions of the world. we quantified the pathogenicity of the chinese isolate if-1106 against different life stages of sweetpotato whitefly, bemisia tabaci, on cucumber plants, and describe the infection process and aspects of the host immunological response in the laboratory. the second instar was the most susceptible life stage to infection, with mortality rates at 10(7)conidia/ ... | 2015 | 26264671 |
| expression of dsrna in recombinant isaria fumosorosea strain targets the tlr7 gene in bemisia tabaci. | rna interference (rnai) technology shows a great potential in controlling agricultural pests, despite the difficulty of introducing exogenous dsrna/sirna into target pests. isaria fumosorosea is a common fungal pathogen of the b-biotype bemisia tabaci (whitefly), which is a widespread pest. entomopathogenic fungi directly penetrate the cuticle and invade insect hemocoel. application of i. fumosorosea expressing dsrna of whitefly immunity-related gene may aid in developing rnai technology to effe ... | 2015 | 26198409 |
| microsatellite variability in the entomopathogenic fungus paecilomyces fumosoroseus: genetic diversity and population structure. | the hyphomycete paecilomyces fumosoroseus (pfr) is a geographically widespread fungus capable of infecting various insect hosts. the fungus has been used for the biological control of several important insect pests of agriculture. however knowledge of the fungus' genetic diversity and population structure is required for its sustainable use as a biological control agent. we investigated length and sequence polymorphisms of nine microsatellite loci for 33 pfr accessions sampled from various host ... | 2008 | 18268903 |
| isolation of dipicolinic acid as an insecticidal toxin from paecilomyces fumosoroseus. | several entomopathogenic fungi produce toxins that could be used as bioinsecticides in integrated pest management programs. paecilomyces fumosoroseus is currently used for the biological control of the whiteflies bemisia tabaci and b. argentifolii. supernatants from submerged batch culture, where the fungus produced abundant dispersed mycelium, conidia and blastospores, were toxic to the whitefly nymphs. the most abundant metabolite was purified by hplc and identified by mass spectrometry and nm ... | 2005 | 15696281 |
| genetic variability of paecilomyces fumosoroseus isolates revealed by molecular markers. | paecilomyces fumosoroseus (deuteromycotina:hyphomycetes) is a fungus that is potentially useful for the bio-control of economically important agricultural pests, such as whitefly (bemisia tabaci). arbitrarily primed pcr and pcr with trna consensus primers were used to analyze genetic variability among 27 p. fumosoroseus isolates, 15 of which came from the same host, b. tabaci, one p. lilacinus isolate, used as an outgroup, 9 previously unidentified paecilomyces isolates. fifteen 10-mer oligonucl ... | 1995 | 7745282 |
| single basal application of thiacloprid for the integrated management of meloidogyne incognita and bemisia tabaci in tomato crops. | tomato growers commonly face heavy nematode (meloidogyne incognita) and whitefly (b-biotype bemisia tabaci) infestations, and previous studies demonstrated that thiacloprid could be used to control m. incognita and b. tabaci in cucumber. however, the efficacy of a single basal application of thiacloprid to control both pests and its effect on yield in tomato remains unknown. in this study, the potential of thiacloprid application to the soil for the integrated control of m. incognita and b. taba ... | 2017 | 28120937 |
| effect of methomyl and oxamyl soil applications on early control of nematodes and insects. | methomyl is a widely used carbamate insecticide that has traditionally been applied as a foliar spray. more recently, methomyl has been labeled as a soil application via drip chemigation. not much is known about the insecticidal and nematicidal potential of soil-applied methomyl. methomyl soil applications were evaluated for their potential to control soil nematodes and foliar insect pests in a series of lab and greenhouse tests. | 2011 | 21438120 |
| heterologous expression of the mi-1.2 gene from tomato confers resistance against nematodes but not aphids in eggplant. | the mi-1.2 gene in tomato (solanum lycopersicum) is a member of the nucleotide-binding leucine-rich repeat (nblrr) class of plant resistance genes, and confers resistance against root-knot nematodes (meloidogyne spp.), the potato aphid (macrosiphum euphorbiae), and the sweet potato whitefly (bemisia tabaci). mi-1.2 mediates a rapid local defensive response at the site of infection, although the signaling and defensive pathways required for resistance are largely unknown. in this study, eggplant ... | 2006 | 16610741 |
| the root-knot nematode resistance gene mi-1.2 of tomato is responsible for resistance against the whitefly bemisia tabaci. | the tomato gene mi-1.2 confers resistance against root-knot nematodes and some isolates of potato aphid. resistance to the whitefly bemisia tabaci previously has been observed in mi-bearing commercial tomato cultivars, suggesting that mi, or a closely linked gene, is responsible for the resistance. the response of two biotypes of b. tabaci to tomato carrying the cloned mi was compared with that of the isogenic untransformed tomato line moneymaker. our results indicate that mi-1.2 is responsible ... | 2003 | 12848430 |
| the allelic relationship of genes giving resistance to mungbean yellow mosaic virus in blackgram. | the allelic relationship of resistance genes for mymv was studied in blackgram (v. mungo (l.) hepper). the resistant donors to mymv - 'pant u84' and 'upu 2', and their f1, f2 and f3 generations - were inoculated artificially using an insect vector, whitefly (bemisia tabaci genn.). the two recessive genes previously reported for resistance were found to be the same in both donors. | 1986 | 24248192 |
| comparison of transmission of papaya leaf curl china virus among four cryptic species of the whitefly bemisia tabaci complex. | begomoviruses are transmitted by cryptic species of the whitefly bemisia tabaci complex, often in a species-specific manner. papaya leaf curl china virus (palcucnv) has been recorded to infect several crops including papaya, tomato and tobacco in china. to help assess the risks of spread of this virus, we compared the acquisition, retention and transmission of palcucnv among four species of whiteflies, middle east-asia minor 1 (meam1), mediterranean (med), asia 1 and asia ii 7. all four species ... | 2015 | 26486606 |
| microarray analysis of tomato plants exposed to the nonviruliferous or viruliferous whitefly vector harboring pepper golden mosaic virus. | plants are routinely exposed to biotic and abiotic stresses to which they have evolved by synthesizing constitutive and induced defense compounds. induced defense compounds are usually made, initially, at low levels; however, following further stimulation by specific kinds of biotic and abiotic stresses, they can be synthesized in relatively large amounts to abate the particular stress. cdna microarray hybridization was used to identify an array of genes that were differentially expressed in tom ... | 2014 | 25525099 |
| the role of bacterial chaperones in the circulative transmission of plant viruses by insect vectors. | persistent circulative transmission of plant viruses involves complex interactions between the transmitted virus and its insect vector. several studies have shown that insect vector proteins are involved in the passage and the transmission of the virus. interestingly, proteins expressed by bacterial endosymbionts that reside in the insect vector, were also shown to influence the transmission of these viruses. thus far, the transmission of two plant viruses that belong to different virus genera w ... | 2013 | 23783810 |
| potato yellow mosaic virus: a synonym of tomato yellow mosaic virus. | tomato yellow mosaic was first described in 1963, as a disease caused by a geminivirus transmitted by the whitefly bemisia tabaci in venezuela. in 1981 and 1985, tomato yellow mosaic virus (toymv) was reported to occasionally infect potato plants growing in the proximity of tomato plantings affected by this virus. despite these previous reports, a virus isolated from yellow mosaic-affected potato plants in venezuela, was described in 1986 as a "new geminivirus" called potato yellow mosaic virus ... | 2001 | 11765926 |
| the immune strategy and stress response of the mediterranean species of the bemisia tabaci complex to an orally delivered bacterial pathogen. | the whitefly, bemisia tabaci, a notorious agricultural pest, has complex relationships with diverse microbes. the interactions of the whitefly with entomopathogens as well as its endosymbionts have received great attention, because of their potential importance in developing novel whitefly control technologies. to this end, a comprehensive understanding on the whitefly defense system is needed to further decipher those interactions. | 2014 | 24722540 |
| host nuclear genotype influences phenotype of a conditional mutualist symbiont. | arthropods commonly carry maternally inherited intracellular bacterial symbionts that may profoundly influence host biology and evolution. the intracellular symbiont rickettsia sp. nr. bellii swept rapidly into populations of the sweetpotato whitefly bemisia tabaci in the south-western usa. previous laboratory experiments showed female-bias and fitness benefits were associated with rickettsia infection, potentially explaining the high frequencies of infection observed in field populations, but t ... | 2017 | 27748992 |
| sequencing and comparison of the rickettsia genomes from the whitefly bemisia tabaci middle east asia minor i. | the whitefly, bemisia tabaci, harbors the primary symbiont 'candidatus portiera aleyrodidarum' and a variety of secondary symbionts. among these secondary symbionts, rickettsia is the only one that can be detected both inside and outside the bacteriomes. infection with rickettsia has been reported to influence several aspects of the whitefly biology, such as fitness, sex ratio, virus transmission and resistance to pesticides. however, mechanisms underlying these differences remain unclear, large ... | 2016 | 27273750 |
| characterization of the symbiont rickettsia in the mirid bug nesidiocoris tenuis (reuter) (heteroptera: miridae). | nesidiocoris tenuis (reuter) (heteroptera: miridae) is an omnivorous insect used for biological control. augmentative release and conservation of n. tenuis have been used for pest control in tomato crops. intracellular bacterial symbionts of arthropods are common in nature and have diverse effects on their hosts; in some cases they can dramatically affect biological control. fingerprinting methods showed that the symbiotic complex associated with n. tenuis includes wolbachia and rickettsia. rick ... | 2014 | 25062354 |
| a bemisia tabaci midgut protein interacts with begomoviruses and plays a role in virus transmission. | begomoviruses are a major group of plant viruses, transmitted exclusively by bemisia tabaci (gennadius) in a persistent circulative non-propagative manner. the information regarding molecular and cellular basis underlying begomovirus - whitefly interaction is very scarce. evidences have suggested that the insect gut possesses some crucial protein receptors that allow specific entry of virus into the insect haemolymph. we have performed yeast two hybrid gut cdna expression library screening again ... | 2016 | 26488331 |
| chlorotic curly stunt: a severe begomovirus disease of bottle gourd in northern india. | bottle gourd (lagenaria siceraria) an important vegetable crop in india was observed to be affected by a chlorotic curly stunt disease (ccsd) during 2003-2006 in the vegetable growing areas of delhi and adjoining state of haryana. the affected plants are severely stunted and bear very small chlorotic and mildly curled leaves. incidence of the disease varied from 4.7 to 36%. the disease could be easily transmitted by whitefly, bemisia tabaci but not by sap. the causal virus was found to be a bego ... | 2010 | 23637479 |
| use of posttranscription gene silencing in squash to induce resistance against the egyptian isolate of the squash leaf curl virus. | squash leaf curl virus (sqlcv) is a bipartite begomovirus affecting squash plants. it is transmitted by whitefly bemisia tabaci biotype b causing severe leaf curling, vein banding, and molting ending by stunting. in this study full-length genomic clone of sqlcv egyptian isolated and posttranscriptional gene silencing (ptgs) has been induced to develop virus resistance. the noubaria sqlcv has more than 95% homology with jordon, israel, lebanon, palestine, and cairo isolates. two genes fragment fr ... | 2016 | 27034922 |
| transmission of squash vein yellowing virus to and from cucurbit weeds and effects on sweetpotato whitefly (hemiptera: aleyrodidae) behavior. | since 2003, growers of florida watermelon [citrullus lanatus (thunb.) matsum. and nakai] have periodically suffered large losses from a disease caused by squash vein yellowing virus (sqvyv), which is transmitted by the whitefly middle east-asia minor 1 (meam1), formerly bemisia tabaci (gennadius) biotype b. common cucurbit weeds like balsam apple (momordica charantia l.) and smellmelon [cucumis melo var. dudaim (l.) naud.] are natural hosts of sqvyv, and creeping cucumber (melothria pendula l.) ... | 2016 | 27400705 |
| control of sweet potato virus diseases. | sweet potato (ipomoea batatas) is ranked seventh in global food crop production and is the third most important root crop after potato and cassava. sweet potatoes are vegetative propagated from vines, root slips (sprouts), or tubers. therefore, virus diseases can be a major constrain, reducing yields markedly, often more than 50%. the main viruses worldwide are sweet potato feathery mottle virus (spfmv) and sweet potato chlorotic stunt virus (spcsv). effects on yields by spfmv or spcsv alone are ... | 2015 | 25591876 |
| infectivity, effects on helper viruses and whitefly transmission of the deltasatellites associated with sweepoviruses (genus begomovirus, family geminiviridae). | begomoviruses (family geminiviridae) are whitefly-transmitted viruses with single-stranded dna genomes that are frequently associated with dna satellites. these satellites include non-coding satellites, for which the name deltasatellites has been proposed. although the first deltasatellite was identified in the late 1990s, little is known about the effects they have on infections of their helper begomoviruses. recently a group of deltasatellites were identified associated with sweepoviruses, a g ... | 2016 | 27453359 |
| natural association of two different betasatellites with sweet potato leaf curl virus in wild morning glory (ipomoea purpurea) in india. | wild morning glory (ipomoea purpurea) was observed to be affected by leaf curl and yellow vein diseases during summer-rainy season of 2009 in new delhi, india. the virus was experimentally transmitted through whitefly, bemisia tabaci to i. purpurea that reproduced the two distinct symptoms. sequence analysis of multiple full-length clones obtained through rolling circle amplification from the leaf curl and yellow vein samples showed 91.8-95.3% sequence identity with sweet potato leaf curl virus ... | 2013 | 23529301 |
| eggplant mild leaf mottle virus (emlmv), a new putative member of the genus ipomovirus that harbors an hc-pro gene. | since 2003, a new viral disease of eggplant (solanum melongena l.) has been spreading in fields in the jordan and arava valleys, israel. the symptoms of this disease include mild leaf mottling and varying degrees of fruit distortion. this disease can be transmitted by mechanical sap inoculation, as well as by the whitefly bemisia tabaci (homoptera, aleyrodidae) and has been tentatively named eggplant mild leaf mottle virus (emlmv). our study aimed to determine the complete sequence and genome or ... | 2012 | 22086504 |
| analysis of the tomato mild mottle virus genome indicates that it is the most divergent member of the genus ipomovirus (family potyviridae). | the complete genome of a tomato mild mottle virus (tommv) isolate was analysed, and some biological features were characterized. the ssrna genome of tommv from ethiopia encompasses 9283 nucleotides (excluding the 3' poly(a) tail) and encodes a polyprotein of 3011 amino acids. phylogenetic and pairwise comparisons with other members of the family potyviridae revealed that tommv is the most divergent member of the genus ipomovirus, with a genome organization similar to that of members of the speci ... | 2012 | 22080188 |
| antioxidant enzyme responses induced by whiteflies in tobacco plants in defense against aphids: catalase may play a dominant role. | bemisia tabaci meam1 (middle east-asia minor 1) feeding alters antioxidative enzyme activity in some plant species. infestation of b. tabaci nymphs decreases myzus persicae performance on systemic, but not local leaves of tobacco plants. however, it is unclear if b. tabaci nymphs induced antioxidant activities contributing to the aphid resistance. | 2016 | 27788203 |
| bensulfuron-methyl treatment of soil affects the infestation of whitefly, aphid, and tobacco mosaic virus on nicotiana tabacum. | bensulfuron-methyl (bsm) is widely used in paddy soil for weed control. bsm residue in the soil has been known to inhibit the growth of sensitive crop plants. however, it is unknown whether bsm residue can affect the agrosystem in general. in this study, we have found significant effects of bsm on the infestation of bemisia tabaci, myzus persicae, and tobacco mosaic virus (tmv) in nicotiana tabacum. the soil was treated with bsm before the pest inoculation. the herbicide-treated tobaccos showed ... | 2016 | 28083007 |
| tomato infection by whitefly-transmitted circulative and non-circulative viruses induce contrasting changes in plant volatiles and vector behaviour. | virus infection frequently modifies plant phenotypes, leading to changes in behaviour and performance of their insect vectors in a way that transmission is enhanced, although this may not always be the case. here, we investigated bemisia tabaci response to tomato plants infected by tomato chlorosis virus (tocv), a non-circulative-transmitted crinivirus, and tomato severe rugose virus (tosrv), a circulative-transmitted begomovirus. moreover, we examined the role of visual and olfactory cues in ho ... | 2016 | 27529271 |
| infectious cdna clones of the crinivirus tomato chlorosis virus are competent for systemic plant infection and whitefly-transmission. | tomato chlorosis virus (tocv) (genus crinivirus, family closteroviridae) causes important emergent diseases in tomato and other solanaceous crops. tocv is not transmitted mechanically and is naturally transmitted by whiteflies. the tocv genome consists of two molecules of linear, positive-sense rna encapsidated into long flexuous virions. we present the construction of full-length cdna clones of the tocv genome (rna1 and rna2) fused to the sp6 rna polymerase promoter and under the control of the ... | 2014 | 25113907 |
| epidemiology and genetic diversity of criniviruses associated with tomato yellows disease in greece. | tomato chlorosis virus (tocv) and tomato infectious chlorosis virus (ticv) are two whitefly transmitted viruses which are classified in the genus crinivirus of the family closteroviridae. both induce similar yellowing symptoms in tomato and are responsible for severe economic losses. tocv is transmitted by bemisia tabaci gennadious, trialeurodes vaporariorum westwood and trialeurodes abutilonea haldeman, whereas ticv is transmitted only by t. vaporariorum. an extensive study was conducted during ... | 2014 | 24370865 |
| molecular detection and partial characterization of a begomovirus causing leaf curl disease of potato in sub-himalayan west bengal, india. | the characteristic disease symptoms of apical leaf curl, crinkled leaves and conspicuous mosaic were observed in potato plants grown in areas of coochbehar, one of the distinct agroclimatic zones of sub-himalayan west bengal. whitefly (bemisia tabaci) population was also observed in and around the infected plants. the characteristic disease symptoms and presence of whitefly (bemisia tabaci) population indicated the possibility of begomovirus infection. total dna was extracted from infected sampl ... | 2014 | 24813019 |
| role of betasatellite in the pathogenesis of a bipartite begomovirus affecting tomato in india. | betasatellites are commonly associated with tomato leaf curl disease caused by begomoviruses in india. this study demonstrates the role of a betasatellite in the pathogenesis of tomato leaf curl new delhi virus (tolcndv), a bipartite begomovirus affecting tomato in india. for infection, accumulation, systemic movement and disease induction by tolcndv, co-infection by the associated betasatellite was not essential, as the dna a alone of tolcndv could infect tomato and nicotiana benthamiana and in ... | 2012 | 22418785 |
| a new begomovirus-betasatellite complex is associated with chilli leaf curl disease in sri lanka. | leaf curl disease of chilli (lcdc) is a major constraint in production of chilli in the indian subcontinent. the objective of this study was to identify the begomovirus species occurring in chilli in sri lanka, where the lcdc was initially recorded in 1938. the virus samples were collected from the north central province, the major chilli growing region in sri lanka with a history of epidemic prevalence of lcdc. the virus could be readily transmitted by bemisia tabaci to chilli, tomato and tobac ... | 2013 | 23090833 |
| sources and inheritance of resistance to leaf curl virus in lycopersicon. | one hundred and twenty-two varieties, lines and wild accessions of lycopersicon were screened under three different regimes during the autumn/winter season of 1982-83 and 1983-84 for resistance to tomato leaf curl virus (tlcv). l. hirsutum f. glabratum ('b6013') and l. hirsutum f. typicum ('a1904') proved to be highly resistant to tlcv in all three environments. various accessions of l. peruvianum were also highly resistant. l. pimpinellifolium ('a1921') exhibited no tlcv symptoms within 90 days ... | 1987 | 24241195 |
| odor, not performance, dictates bemisia tabaci's selection between healthy and virus infected plants. | although, insect herbivores are generally thought to select hosts that favor the fitness of their progeny, this "mother-knows-best" hypothesis may be challenged by the presence of a plant virus. our previous study showed that the whitefly, bemisia tabaci, the obligate vector for transmitting tomato yellow leaf curl virus (tylcv), preferred to settle and oviposit on tylcv-infected rather than healthy host plant, datura stramonium. the performances of b. tabaci larvae and adults were indeed improv ... | 2017 | 28360861 |
| tomato spotted wilt virus infection reduces the fitness of a nonvector herbivore on pepper. | plant pathogens and insect herbivores often share hosts under natural conditions. hence, pathogen-induced changes in a host plant can affect the herbivore and vice versa. even though plant viruses are ubiquitous in the field, little is known about plant-mediated interactions between viruses and nonvector herbivores. here we tested whether the performance of the sweet potato whitefly, bemisia tabaci (gennadius) biotype q, was altered when raised on pepper infected with tomato spotted wilt virus ( ... | 2013 | 23786083 |
| torradoviruses are transmitted in a semi-persistent and stylet-borne manner by three whitefly vectors. | members of the genus torradovirus (family secoviridae, type species tomato torrado virus, totv) are spherical plant viruses transmitted by the whitefly species trialeurodes vaporariorum and bemisia tabaci. knowledge on the mode of vector transmission is lacking for torradoviruses. here, the mode of transmission was determined for tomato marchitez virus (tomarv). a minimal acquisition access period (aap) and inoculation access period (iap) of approximately 2h each was required for its transmissio ... | 2014 | 24342139 |
| rna interference of an antimicrobial peptide, btdef, reduces tomato yellow leaf curl china virus accumulation in the whitefly bemisia tabaci. | the whitefly bemisia tabaci (gennadius) is considered one of the main pests for agriculture. one important problem with the whitefly is its notorious status as a vector for plant viruses, primarily begomoviruses. we have previously identified a defensin-like antimicrobial peptide, btdef, from the whitefly b. tabaci meam1. however, the function of btdef in the immune system of the insect vector and begomovirus transmission has yet to be explored. | 2016 | 27804213 |
| differential profiles of direct and indirect modification of vector feeding behaviour by a plant virus. | plant viruses interact with their insect vectors directly and indirectly via host plants, and this tripartite interaction may produce fitness benefits to both the vectors and the viruses. our previous studies show that the middle east-asia minor 1 (meam1) species of the whitefly bemisia tabaci complex improved its performance on tobacco plants infected by the tomato yellow leaf curl china virus (tylccnv), which it transmits, although virus infection of the whitefly per se reduced its performance ... | 2015 | 25567524 |
| virulence factors of geminivirus interact with myc2 to subvert plant resistance and promote vector performance. | a pathogen may cause infected plants to promote the performance of its transmitting vector, which accelerates the spread of the pathogen. this positive effect of a pathogen on its vector via their shared host plant is termed indirect mutualism. for example, terpene biosynthesis is suppressed in begomovirus-infected plants, leading to reduced plant resistance and enhanced performance of the whiteflies (bemisia tabaci) that transmit these viruses. although begomovirus-whitefly mutualism has been k ... | 2014 | 25490915 |
| specific cells in the primary salivary glands of the whitefly bemisia tabaci control retention and transmission of begomoviruses. | the majority of plant viruses are vectored by arthropods via persistent-circulative or noncirculative transmission. previous studies have shown that specific binding sites for noncirculative viruses reside within the stylet or foregut of insect vectors, whereas the transmission mechanisms of circulative viruses remain ambiguous. here we report the critical roles of whitefly primary salivary glands (psgs) in the circulative transmission of two begomoviruses. the middle east asia minor 1 (meam1) s ... | 2014 | 25210181 |
| molecular characterization of soluble and membrane-bound trehalases of the whitefly, bemisia tabaci. | trehalases (tres) have been demonstrated to be the key enzymes that are involved in various trehalose-associated physiological processes in insects. however, little attention has been devoted to the tres in the whitefly, bemisia tabaci. in this study, a soluble tre (bttre-1) and a membrane-bound tre (bttre-2) were cloned in the invasive cryptic species middle east-asia minor 1 (meam1) of the whitefly b. tabaci complex. alignment of deduced amino acids sequences of both bttres revealed that they ... | 2014 | 24610752 |
| feeding on a begomovirus-infected plant enhances fecundity via increased expression of an insulin-like peptide in the whitefly, meam1. | the middle east-minor 1 cryptic species (meam1), bemisia tabaci (gennadius) is a globally invasive pest. it spreads widely due to its high fecundity and mutualistic interactions with the virus they vector. feeding on virus (tomato yellow leaf curl china virus, tylccnv)-infected host plants improves their fecundity, however, the key factor regulating the signaling transduction in reproduction of whitefly remains to be identified. here, we cloned a full length cdna encoding an insulin-like peptide ... | 2014 | 24532462 |
| detoxification activity and energy cost is attenuated in whiteflies feeding on tomato yellow leaf curl china virus-infected tobacco plants. | the begomovirus tomato yellow leaf curl china virus (tylccnv) can benefit its vector, the whitefly bemisia tabaci, through suppressing the defences of their shared host plants. however, the mechanisms of this vector-virus mutualism remain largely unknown on the insect side of the interaction. here, we compared the transcriptional profiles of female adult whiteflies of b. tabaci middle east-asia minor 1 feeding on tylccnv-free and tylccnv-infected tobacco plants using the next-generation sequenci ... | 2013 | 23889516 |
| enhanced vitellogenesis in a whitefly via feeding on a begomovirus-infected plant. | the meam1 (b biotype) bemisia tabaci (gennadius) is one of the most widespread and damaging whitefly cryptic species. our previous studies discovered that the meam1 whitefly indirectly benefits from interactions with the tomato yellow leaf curl china virus (tylccnv) via accelerated ovarian development and increased fecundity. however, the physiological mechanism of begomoviruse-infected plants acting on the reproduction of the insect vector was unknown. | 2012 | 22937062 |
| emergence and diversity of begomoviruses infecting solanaceous crops in east and southeast asia. | over the past three decades diseases caused by whitefly-transmitted geminiviruses (begomoviruses) have emerged to be important constraints to the production of solanaceous crops, particularly tomato (solanum lycopersicum) and peppers (capsicum spp.), in many tropical and subtropical regions of the world. the most studied of these is tomato yellow leaf curl virus (tylcv), which has spread to many other areas from its likely origin in the mediterranean basin region. the virus is usually associated ... | 2014 | 24440320 |
| the non-canonical tomato yellow leaf curl virus recombinant that displaced its parental viruses in southern morocco exhibits a high selective advantage in experimental conditions. | recombination events are frequently inferred from the increasing number of sequenced viral genomes, but their impact on natural viral populations has rarely been evidenced. tylcv-is76 is a recombinant (begomovirus,geminiviridae) between the israel strain of tomato yellow leaf curl virus (tylcv-il) and the spanish strain of tomato yellow leaf curl sardinia virus (tylcsv-es) that was generated most probably in the late 1990s in southern morocco (souss). its emergence in the 2000s coincided with th ... | 2016 | 27902403 |
| real-time pcr protocols for the quantification of the begomovirus tomato yellow leaf curl sardinia virus in tomato plants and in its insect vector. | tomato yellow leaf curl sardinia virus (tylcsv) (geminiviridae) is an important pathogen, transmitted by the whitefly bemisia tabaci, that severely affects the tomato production in the mediterranean basin. here, we describe real-time pcr protocols suitable for relative and absolute quantification of tylcsv in tomato plants and in whitefly extracts. using primers and probe specifically designed for tylcsv, the protocols for relative quantification allow to compare the amount of tylcsv present in ... | 2015 | 25287496 |
| virion stability is important for the circulative transmission of tomato yellow leaf curl sardinia virus by bemisia tabaci, but virion access to salivary glands does not guarantee transmissibility. | the capsid protein (cp) of the monopartite begomovirus tomato yellow leaf curl sardinia virus (tylcsv), family geminiviridae, is indispensable for plant infection and vector transmission. a region between amino acids 129 and 152 is critical for virion assembly and insect transmissibility. two previously described mutants, one with a double q129p q134h mutation (pnhd) and another with a further d152e change (pnhe), were found nontransmissible (nt). another nt mutant with a single n130d change (qd ... | 2009 | 19321611 |