| human in vitro experimental model for cd4+ t cell targeted immunotherapy to house dust mite. | cd4+ t cells play a critical role in the initiation and potentiation of the allergic immune response. development of allergen-mediated hyposensitization therapies directed at this cell type requires two key steps: (1) identification of the epitopes that are crucial for activating the t-cell response and (2) definition of the specificity of the hla-d region molecules that restrict t-cell recognition of these epitopes. we have located the major t-cell determinants of the group ii allergen of the h ... | 1993 | 7690528 |
| augmentation of permeability in the bronchial epithelium by the house dust mite allergen der p1. | sequence analyses have revealed the existence of homology between certain aeroallergens and proteolytic enzymes. this homology can be expressed functionally, but its significance to airway pathophysiology is unknown. studies with madin-darby canine kidney cells and canine tracheal epithelial cells grown on plastic substrata or matrix proteins suggest that der p1, a major allergen from the house dust mite dermatophagoides pteronyssinus and a cysteine proteinase, or the unfractionated growth mediu ... | 1995 | 7695916 |
| sensitization to inhalant allergens in wheezing infants is predictive of the development of infantile asthma. | early recognition of infantile asthma in wheezing infants is a major problem for physicians. we investigated whether detection of early sensitization to inhalant allergens would be useful to identify those wheezing infants who are likely to develop asthma. a total of 67 infants (aged 1-25 months) hospitalized for a wheezing episode were initially tested for reactivity to inhalant allergens by both skin prick test and in vitro measurement of specific ige antibodies (phadiatop). thirty-seven of th ... | 1994 | 7709993 |
| house dust mite (dermatophagoides farinae and dermatophagoides pteronyssinus) prevalence in the rooms and hallways of a tertiary care hospital. | house dust mites, dermatophagoides farinae and dermatophagoides pteronyssinus, are important components in the development of asthma. | 1995 | 7722158 |
| dietary antioxidant vitamin intake and lung function in the general population. | we have investigated the relation between lung function and dietary intake of the antioxidant vitamins c and e in the general population in a cross-sectional survey of a random sample of adults from the electoral register of an administrative area of nottingham. in 2,633 subjects 18 to 70 yr of age, we measured fev1 and fvc, allergen skin sensitivity to grass pollen, cat fur, and dermatophagoides pteronyssinus, pack-years smoking exposure by personal recall, and usual dietary intake of vitamins ... | 1995 | 7735589 |
| canine atopic disease: the prevalence of positive intradermal skin tests at two sites in the north and south of great britain. | results of intradermal skin test responses to the same panel of 53 allergens were compared in 118 dogs with atopic disease presented at two geographical centres, edinburgh (87 cases) and london (31 cases). the allergens most commonly positive at both centres were human dander and dermatophagoides farinae, but positive tests to all of the allergens used occurred in at least one case. the mean number of allergens to which positive tests resulted in atopic dogs was 5.126 (edinburgh) and 5.129 (lond ... | 1995 | 7747407 |
| il-10 secretion of allergen-specific skin-derived t cells correlates positively with that of the th2 cytokines il-4 and il-5. | in atopic individuals, allergen-specific cd4+ t lymphocytes often belong to the t-helper 2 (th2) subset as they secrete the marker cytokines interleukin-4 (il-4) and il-5 but not interferon-gamma (inf-gamma). il-10 is a cytokine the production of which, in the mouse system has been described to be restricted to the th2 subset, but in the human was found to be produced by both th1 and th2 t cell clones (tcc). we have recently shown that house dust mite antigen (dermatophagoides pteronyssinus)-spe ... | 1994 | 7749574 |
| natural history of ige antibodies in children at risk for atopy. | the relationship between atopic diseases and high serum ige levels has been emphasized since the mid-sixties, when ige antibodies were discovered. a number of environmental factors, such as the type of feeding, passive smoking, viral infections, early mold, house dust mite and pet fur exposure modulate ige synthesis and affect the development of atopic diseases. | 1995 | 7749975 |
| mite antigen in house dust: relationship with different housing characteristics in the netherlands. | as part of a case-controlled study on the relationship between home dampness and respiratory symptoms of children, the concentration of the major allergen of dermatophagoides pteronyssinus (der p i) in floor dust and mattress dust in 516 dwellings in the netherlands was measured. a checklist, completed by the investigators, was used to obtain information on home and occupant characteristics, which may have an impact on the der p i concentration in house dust. the geometric mean mite antigen conc ... | 1994 | 7812886 |
| comparison of two epidemiological protocols for measuring airway responsiveness and allergic sensitivity in adults. | in recent years, airway responsiveness has commonly been measured in epidemiological studies using one of two methods. in one method, histamine is administered via a handheld devilbiss nebulizer and in the other, methacholine is administered via a dosimeter. allergic sensitivity has commonly been measured by either the allergen droplet method or by phazet. we wanted to assess the comparability of airway responsiveness and of allergic sensitivity measured by both methods. a total of 48 volunteers ... | 1994 | 7828688 |
| atopic sensitization in children of somali immigrants in italy. | children of immigrants are known to be at greater risk for developing allergic manifestations than the population in general. in this study we observed children of somali origin living in italy in order to find plausible explanations for the high risk of allergic disease in immigrants. fifty-two children aged 0-14 years (mean = 6.7 years) were examined during the spring season. the mothers of the children were asked to fill in a questionnaire on symptoms and signs of atopic diseases and the fami ... | 1994 | 7850033 |
| relationship between facilitated allergen presentation and the presence of allergen-specific ige in serum of atopic patients. | allergen presentation to allergen-specific t cells can be facilitated when ige-allergen complexes are endocytosed by antigen-presenting cells (apc) after binding to the low-affinity fc epsilon r type ii (cd23). here we present a study on the relative capabilities of sera of atopic patients to mediate facilitated antigen presentation (fap). to this aim fap was studied in an in vitro model in which cd23-expressing epstein-barr virus (ebv)-b cells act as apc to t lymphocyte clones (tlc) that are sp ... | 1995 | 7851024 |
| house dust mite allergen (der p i) and respiratory symptoms in children: a case-control study. | the association between house dust mite allergen in house dust and childhood respiratory symptoms was investigated in a case-control study of 259 children with reported chronic respiratory symptoms and 257 control children without reported respiratory symptoms. the der p i concentration in floor dust of the living room and bedroom and in mattress dust was determined using an enzyme immunoassay. venous blood samples were taken from all children for serum ige determination against house dust mite ... | 1994 | 7874605 |
| the influence of increased bronchial responsiveness, atopy, and serum ige on decline in fev1. a longitudinal study in the elderly. | we have performed a 4-yr prospective survey of the association of allergen skin test positivity, total serum immunoglobulin e (ige), and bronchial responsiveness to methacholine, with longitudinal decline in fev1, in subjects over 65 yr of age. in 1987, 324 subjects completed a respiratory questionnaire, and underwent measurement of fev1 and fvc, methacholine challenge, skin prick testing (to dermatophagoides pteronyssinus, cat fur, mixed grasses, and aspergillus fumigatus) and estimation of tot ... | 1995 | 7881652 |
| cow milk allergy within the spectrum of atopic disorders. | in order to examine the relationship between cow milk allergy (cma) and atopic disorders in childhood, a consecutive group of 42 infants with ige mediated cma was followed for at least 2 years. the incidence of sensitization to common food and inhalant antigens and the development of eczema, asthma, and food allergies was examined for the cohort and compared between patients whose cma remitted and those with persistent disease. in this cohort the prevalence of eczema was 57%, asthma 69%, egg all ... | 1994 | 7889427 |
| genetic linkage of t-cell receptor alpha/delta complex to specific ige responses. | ige responses to inhaled proteins underlie the clinical syndrome of allergic (atopic) asthma and rhinitis. we have investigated genetic linkage between specific ige reactions to highly purified major allergens and the t-cell receptor (tcr) alpha and beta gene complexes on chromosome 14 and 7, respectively. antigens tested included highly purified proteins from the housedust mite dermatophagoides pteronyssinus, the domestic cat and dog, grass pollen, and the mould alternaria alternata. affected s ... | 1994 | 7911920 |
| evaluation of the potency of allergenic extracts by inhibition of igg-antibody binding. | a method is described for the in vitro potency evaluation of allergenic extracts by using their capacity of binding to specific human igg antibodies. the results obtained with this inhibition-type enzyme immunoassay are compared with the analyses by the customary method of ige antibody binding. a large series of allergenic protein fractions from the pollen of the gramineae, olea europea, parietaria judaica, and from the dust mite dermatophagoides pteronyssinus as well as from cat dander and pean ... | 1994 | 7917232 |
| cloning and characterization of a major allergen of the house dust mite, dermatophagoides pteronyssinus, homologous with glutathione s-transferase. | a major allergen of the house dust mite, dermatophagoides pteronyssinus, has been identified and characterized from a lambda gt11 cdna library of the mite. ige antibodies from the sera of allergic patients that recognise the cloned polypeptide bind to an approximately 26 kda polypeptide on a western blot of reduced mite polypeptides. nucleotides sequencing of the clone revealed a 219 amino acid open reading frame encoding a protein with a derived molecular mass of 25,589 da and a pi of 6.3. comp ... | 1994 | 7918651 |
| human ige in scid mice reconstituted with peripheral blood mononuclear cells from dermatophagoides pteronyssinus-sensitive patients. | scid mice were reconstituted with purified peripheral blood mononuclear cells from patients sensitive to dermatophagoides pteronyssinus (dpt). after immunization with a low dose of the related allergen, these human (hu)-scid mice may develop a specific ige response. by using an ige-dependent platelet assay and the related allergen dpt, the human ige was demonstrated to be functional. indeed a high correlation was obtained between both parameters. no rise in human ige level was detected within 3 ... | 1994 | 7930597 |
| dietary sodium intake and the risk of airway hyperreactivity in a random adult population. | high dietary sodium intake has been identified as a potential cause of asthma and airway hyperreactivity. this study was designed to test the hypothesis that dietary sodium intake is an independent determinant of the risk of hyperreactivity in the general population, and to assess the role of atopy in the association between these factors. | 1994 | 7940426 |
| comparison of three in vitro assays for serum ige with skin testing in asthmatic children. | the diagnostic performance of three commercial assay kits [phadezym rast (phrast), pharmacia cap system (cap), and multiple chemiluminescent assay (cla-mast)] for measuring serum-specific ige was evaluated and compared using intradermal skin testing or skin prick testing as reference standards. serum samples were obtained from allergic patients who were tested with either intradermal skin tests or skin prick tests (96 and 49 subjects, respectively). six different allergen extracts were tested: d ... | 1994 | 7944001 |
| [breeding control and immobilizing effects of wood microingredients on house dust mites]. | the possible effects of essential oils as wood microingredients on house dust mites (tyrophagus putrescentiae, dermatophagoides farinae and dermatophagoides pteronyssinus) were investigated. 1. whether small pieces of 5 types of wood had any control effect on mite breeding was studied. mite breeding using only normal feed was compared with breeding using feed mixed with small pieces of wood. in addition, mite breeding using feed mixed with small pieces of wood with no essential oil was studied. ... | 1994 | 7949285 |
| effect of regular terbutaline and budesonide on bronchial reactivity to allergen challenge. | there is a transient rebound increase in bronchial reactivity to histamine and methacholine following regular treatment with an inhaled beta 2-agonist. we set out to determine whether the response to allergen was increased after cessation of regular inhaled terbutaline and whether concomitant inhaled budesonide modifies this response. in a double-blind, double-dummy, parallel group design we studied 41 subjects (37 evaluable) with mild asthma who were allergic to dermatophagoides pteronyssinus, ... | 1994 | 7952551 |
| mite allergen exposure is a risk for the incidence of specific sensitization. | mite allergen exposure is essential for the process of specific allergic sensitization. however, it is not clear whether incidence of specific sensitization at school age depends on the level of mite antigen exposure. therefore the relationship between mite allergen exposure and incidence of specific sensitization was investigated. | 1994 | 8027498 |
| do latex allergens potentiate other skin tests? | we report the case of a 33 year old atopic female patient, who worked for the last 4 years as a cleaner in the surgical area of a hospital. one year after starting the work in this area, the patient noticed upper respiratory symptoms and soon later dyspnea immediately after entering the operating room. other allergic symptoms were perennial rhinoconjunctivitis and urticaria after contact with peaches. common skin prick tests were positive for dermatophagoides pteronyssinus. fifteen min later a s ... | 1994 | 8030583 |
| asthma, allergy and atopy in southern chinese school students. | in order to determine the prevalence of asthma, allergy and atopy in southern china and the relative importance of atopy and parental history in predicting asthma and allergic diseases, we carried out a cross-sectional study on 737 secondary school students aged 12-20 (492 boys, 245 girls; mean age = 16.4 years, sd = 1.8 years) in the city of san bu, guangdong, china. standard questionnaires on respiratory and allergic symptoms were distributed for completion by parents with an overall response ... | 1994 | 8039021 |
| ige to food allergens are highly prevalent in patients allergic to pollens, with and without symptoms of food allergy. | serum samples from 274 patients allergic to one or more of three pollens (birch, grass, mugwort), from 36 patients allergic to cat and/or dermatophagoides pteronyssinus but not to pollen and from 55 non-allergic controls, as well as 20 cord blood samples, were examined for specific ige to six 'pollen-associated' food allergens by using a new sensitive assay (cap). a questionnaire asking for reactions to food was also sent to all patients. in the pollen group, 111 patients (47%) were positive (> ... | 1994 | 8039023 |
| isolation of three species of mites from house dust of atopic dermatitis patients in qualyobia governorate, egypt. | dermatitis or inflammation of the skin caused by an outside agent, is a condition with many causes. it may result from direct irritation of the skin by a substances (chemical or insecticide) or it may be an allergic reaction to a particular substance that has been in contact with the skin as soap or detergent or insect urticating hairs; injected as insect saliva or faeces or sting or taken by mouth as food or drug. in general, treatment of dermatitis depends mainly upon the cause. in the present ... | 1994 | 8077751 |
| interleukin-1 favours the in vitro development of type 2 t helper (th2) human t-cell clones. | the effects exerted by interleukin-1 (il1) on the growth and differentiation of human th1 and th2 cells were examined. neither il1 nor the il1 receptor antagonist (il1ra) had detectable activity toward the antigen- or anti-cd3 antibody-induced proliferative response of already established type 1 t helper (th1) or type 2 t helper (th2) clones. moreover, neither exogenous il1 addiction to, nor neutralization of, endogenously produced il1 in bulk cultures before cloning changed the th1-like cytokin ... | 1994 | 8079050 |
| quantitative analysis of peripheral allergen-specific b lymphocytes in asthma. | we have recently shown that the number of peripheral allergen-specific t cells can, in part, predict, together with methacholine hyperresponsiveness, the bronchial response to inhaled allergen in asthmatic patients. this study was designed to explore the role of blood b cells committed to produce allergen-specific ige in asthma. twenty-three asthmatic patients sensitized to dermatophagoides pteronyssinus and 11 control subjects were studied. peripheral blood b cells, committed to produce allerge ... | 1994 | 8092432 |
| asthma in gaza refugee camp children and its relationship with house dust mites. | three hundred asthmatic children aged 3 to 15 years, and 100 age-sex-neighborhood matched controls, were studied for environmental risk factors for asthma during 1986 to 1987. in 98% of the asthmatic patients, exposure to house dust aggravated the symptoms. in 97% of the cases, the symptoms were more severe at home; in more than 50%, the symptoms persisted throughout the year. significantly more cigarettes were smoked in the homes of the allergic children than in the control homes. house dust sa ... | 1994 | 8109806 |
| patch test reactions to mite antigens: a gerda multicentre study. groupe d'etudes et de recherches en dermato-allergie. | we performed patch tests with dermatophagoides pteronyssinus (dp) antigens from 2 different sources in 355 non-randomly selected patients with atopic dermatitis (ad) and 398 subjects of a control group. the study demonstrated that contact sensitization to mites occurred in an appreciable % of ad cases (20.8%), using commonly available assay products. the differences recorded between the 2 materials tested were related to the concentration of p1 antigen. non-atopic patients rarely showed positive ... | 1993 | 8112063 |
| immunobiology of the serine protease allergens from house dust mites. | extracts of dermatophagoides pteronyssinus and d. farinae were shown to contain a variety of 30 kda serine proteases, including trypsin, chymotrypsin, and an elastase-like enzyme. the mite trypsin, unlike chymotrypsin and the elastase enzyme, was heterogeneous with regard to charge. the enzymes were shown to be present at higher concentration in fecally enriched extracts than in whole mite extracts. the proteases were shown to induce vascular permeability and to detach cells in tissue culture. f ... | 1994 | 8116631 |
| prevalence of patent and latent atopy among a general normal adult population in the south east of france by rast investigation and correlation with circulating total ige levels. | a population of 512 adults (men: 266, 52%, women: 246, 48%) with mean age 41.4 years (s.d. 10.7) was investigated. this population was subdivided into five groups: marseilles city, manosque a small town in a prealpine area, the area surrounding the etang de berre, a rural area in northern bouches du rhône and chateaurenard, a small town in an intensive agricultural area. rasts to a panel of eight allergens (lolium perenne, parietaria officinalis, cupressus sempervirens, olea europea, cat dander, ... | 1993 | 8160568 |
| abnormal igg4 antibody response to aeroallergens in allergic patients. | various studies have suggested the involvement of immunoglobulin g4 (igg4) antibodies (ab) in the physiopathology of allergic disorders. recently, an abnormal igg4 ab production in response to immunization has been reported in some atopic patients. thus, in order to evidence in allergic patients, a potential abnormal igg4 ab response to aeroallergens following natural exposure, we compared, in 34 patients sensitive to dermatophagoides pteronyssinus and in 16 healthy subjects, the igg4 ab respons ... | 1994 | 8199463 |
| antigen presentation by human dermal microvascular endothelial cells. immunoregulatory effect of ifn-gamma and il-10. | human dermal microvascular endothelial cells (hdmec) play a central role in many aspects of the inflammatory and immune reactions in skin. hdmec display a phenotypic diversity ranging from cells with an epithelioid morphology to those that show both morphologic and biochemical characteristics of macrophages. here it is shown that hdmec possess the capability to both process and present ags. t lymphocyte clones specific for peptide p94-104, which are derived from the protein of group i allergen o ... | 1994 | 8207203 |
| sensitization to four common inhalant allergens within 302 nuclear families. | the coincidence of allergic sensitization was investigated in 302 school-aged children and their parents. specific sensitization to four common inhalant allergens (grass and birch pollens, cat dander, dermatophagoides pteronyssinus) was ascertained by means of skin-prick tests (spt) carried out on the complete family unit at the beginning of a 22-month follow-up period. the same test procedure was then repeated on the children twice at 11-month intervals to provide cumulative prevalences of sens ... | 1993 | 8221260 |
| cd23 expression on b-lymphocytes and its modulation by cytokines in allergic patients. | the aim of this study was to assess the expression of cd23 on peripheral blood b-cells, and its in vitro modulation by recombinant human interferon-gamma (ifn-gamma) in phytohaemagglutinin-(pha) or recombinant human interleukin-4 (il-4)-stimulated cultures in atopic patients with dermatophagoides pteronyssinus hypersensitivity and in healthy non-atopic subjects. atopic patients with asthma not receiving allergen-specific immunotherapy (n = 21) were studied and further compared with a group of at ... | 1993 | 8221262 |
| characterization and partial purification of the croatian national standard dermatophagoides pteronyssinus allergen extract. | lyophilized dermatophagoides pteronyssinus (der p) allergen extract (ae) and partially purified der p extract (pae) were prepared and characterized. partial purification of ae was performed by gel filtration on sephadex g-100 and sephacryl s-300. crossed immunoelectrophoresis (cie) disclosed the same precipitating lines in ae and pae preparations. the relative potencies of ae and pae were determined and compared with the who international standard for der p by the rast inhibition method. the pot ... | 1993 | 8238802 |
| a different profile of epitopic dominance in the immunoglobulin g response to bovine betalactoglobulin in lung cancer. | the authors previously documented a quantitative defect in the immunoglobulin g (igg) response toward bovine betalactoglobulin (blg), the major cow's milk antigen, and antigen p1 of the house dust mite, dermatophagoides pteronyssinus (der p1), in patients with lung cancer. in the der p1 model, the authors documented at the igg level an epitope specificity that differed between patients with lung cancer (preferential specificity for cryptic epitopes) and healthy control subjects and patients with ... | 1993 | 8252475 |
| study of the sensitization to storage mites in a pediatric population in barcelona. | we performed the following study in order to determine the prevalence and characteristics of sensitization to storage mites in children mostly from the city of barcelona. all children over 3 years of age attending our unit for the first time due to respiratory problems were given cutaneous tests using the prick test for the house dust mites dermatophagoides pteronyssinus and d. farinae, and the storage mites acarus siro, lepidoglyphus destructor and tyrophagus putrescentiae. we carried out a ras ... | 1993 | 8281346 |
| mite allergy in the tropics: sensitization to six domestic mite species in cartagena, colombia. | sensitization to the domestic mites blomia tropicalis, chortoglyphus arcuatus, lepidoglyphus destructor and aleuroglyphus ovatus was investigated in 77 subjects with clinical symptoms of asthma and/or allergic rhinitis and a positive skin prick test (wheal > or = 3 mm) to dermatophagoides pteronyssinus and/or d. farinae. seventy patients were skin test-positive to d. pteronyssinus and 73 to d. farinae. serum samples from 29 nonallergic, skin test-negative individuals were used as negative contro ... | 1993 | 8281353 |
| evaluation of the overall degree of sensitization to airborne allergens by a single serologic test: implications for epidemiologic studies of allergy. | in most epidemiologic studies of respiratory allergy, the overall degree of sensitization is usually measured by indices based on skin prick test reactions to a panel of relevant airborne allergens. in the present study we used a single assay (cap-phadiatop, pharmacia, uppsala, sweden), which is based on the reaction between serum-specific ige and a mixture of locally relevant inhalant allergens to measure the degree of sensitization to inhalants in the sera of 1815 young italian men, in whom a ... | 1994 | 8308184 |
| mite fauna, der p i, der f i and blomia tropicalis allergen levels in a tropical environment. | fifty dust samples were collected from the mattresses and bedroom floors of 25 subjects with allergic asthma in cartagena, colombia, in order to identify house dust mites and quantitate der p i, der f i and blomia tropicalis allergens. the geometric mean of the total mite density per gram of dust was 418 (range, 40-2280). twenty-two samples (44%) had more than 500 mites and four, less than 100. b. tropicalis and dermatophagoides pteronyssinus were found in 96% and 90% of the samples, accounting ... | 1993 | 8319126 |
| evaluation of allergen-specific ige antibodies by mast for the diagnosis of nasal allergy. | multiple-antigen simultaneous test (mast) is a new system for detecting allergen-specific ige antibodies. multiple antigens can be examined simultaneously in a short period of time by this method. the purpose of this study is to evaluate the usefulness of this method and to compare the results of mast with those of rast and intradermal skin test using 133 serum samples obtained from patients with nasal allergy. the positive rates of the main allergens detected by the mast system are 56% for japa ... | 1993 | 8321979 |
| screening for ige mediated allergy among people working in the marseilles harbour. | screening for ige mediated allergy by rasts to professional (castor bean, green coffee, peanut, soy protein, wheat, rice), and non professional (pollens, mites, cat, alternaria tenuis) air borne antigens among 36 people working in the marseilles harbour has showed rather unexpected findings: only one case of ige positivity to dermatophagoides pteronyssinus (class i) and one case of ige positivity to castor bean seed (ricinus communis) (class iv). igg4 specific antibodies against castor bean and ... | 1993 | 8328353 |
| allergic skin tests and respiratory diseases in 1612 subjects. | in 1985-90, 1612 subjects were submitted to allergic tests for respiratory diseases. in a retrospective survey we have evaluated clinical features, symptoms, diagnostic procedure and allergic tests of skin positives and skin negatives. prick tests were supplemented by intradermal reaction and rast under guideline of history. allergens examined were gramineae, parietaria, olea, house dust, dermatophagoides farinae and pteronyssinus. 80% of patients were found positive to one or more allergens; th ... | 1993 | 8328355 |
| analysis of human t cell responses to the group ii allergen of dermatophagoides species: localization of major antigenic sites. | ige antibodies reactive with the group ii allergens of dermatophagoides species (house dust mite [hdm]) are a major component of the allergic immune response in hdm-allergic atopic individuals. | 1993 | 8335846 |
| igg and ige antibody responses to dust mite allergens among children with asthma in brazil. | we compared exposure and levels of ige and igg antibodies to inhalant allergens in 20 children with asthma and positive skin tests to dermatophagoides pteronyssinus, living in são paulo, to 20 control children without history of asthma or allergy and negative skin tests to d. pteronyssinus, d. farinae, and blomia tropicalis. in 16/20 and 17/20 houses of asthmatic and control children, respectively, at least one sample was obtained which contained > 10 micrograms der p i/g of dust. serum ige anti ... | 1993 | 8346869 |
| influence of low temperature (-30 degrees c) on the different stages of the human allergy mite dermatophagoides pteronyssinus (acari: epidermoptidae). | effects of low temperature (-30 degrees c) on the different life stages of dermatophagoides pteronyssinus (trouessart) were studied. humid cold air at -30 degrees c killed all stages of mites except eggs after 10 min; after 35 min exposure, 20% of the eggs hatched after the incubation period. dry, cold air at -30 degrees c killed 40% of all stages of mites except eggs after 4 min and 100% after 5 min; all eggs died after 1 min exposure. | 1993 | 8360908 |
| effects of the fungus aspergillus penicillioides on the house dust mite dermatophagoides pteronyssinus: an experimental re-evaluation. | in this report the widely-held view that house dust mites benefit from fungal contamination of the dietary substratum is re-examined. the performance of dermatophagoides pteronyssinus (acari: pyroglyphidae) is documented over two successive generations in the presence or absence of the xerophilic fungus aspergillus penicillioides (hyphomycetales: moniliaceae). this fungus reduced survival, development rate, adult length and fecundity of d. pteronyssinus. detrimental effects of a. penicillioides ... | 1993 | 8369562 |
| [a comparative study on skin tests and allergen-specific ige levels determined by enzyme allergo-sorbent testing (east)]. | ninety seven randomly selected subjects with bronchial asthma and allergic rhinitis were classified for the comparative studies. the level of allergen specific ige determined by enzyme allergo-sorbent testing (east) was compared with skin tests. the statistically significant correlation was found for 9 selected allergens. however, the spearman's rank correlation coefficient values varied largely, depending on a given allergen. they ranged from 0.74 (dermatophagoides pteronyssinus) to 0.33 (birch ... | 1993 | 8401337 |
| relationship between exposure to dust mite allergen and bronchial response to exercise in schoolchildren sensitized to dust mites. | we assessed the relationship between the exposure to dust mite allergens and a bronchial response to exercise in 8-year-old schoolchildren. dust was collected from the mattresses of 1,291 children and the concentration of mite allergens was estimated by a commercially available elisa test using monoclonal antibodies (alk, copenhagen) against the major allergens of dermatophagoides pteronyssinus (der pt) and dermatophagoides farinae (der f). a positive bronchial response to exercise (decrease of ... | 1993 | 8414735 |
| antibody affinity disturbance in the immunoglobulin immune response to mucosal antigenic stimulation in lung cancer. the betalactoglobulin model. | this study is a continuation of a recent study, in which a defect in the immunoglobulin g (igg) response to some natural antigens (bovine betalactoglobulin [blg] from cow's milk and antigen p1 from the house dust mite dermatophagoïdes pteronyssinus), usually presented at the mucosal level, was documented in lung cancer patients. the present study further characterizes this difference in terms of antibody relative functional affinity in the blg model. | 1993 | 8422625 |
| sensitization to chortoglyphus arcuatus and aleuroglyphus ovatus in dermatophagoides spp. allergic individuals. | the prevalence of specific ige to the storage mites aleuroglyphus ovatus (ao) and chortoglyphus arcuatus (ca) was studied in 77 individuals with allergic asthma and/or chronic allergic rhinitis. all these individuals had a positive skin test (weal > or = 3 mm) to extracts of dermatophagoides pteronyssinus (dp) and/or dermatophagoides farinae (df). sera from 29 non-atopic individuals were used as controls. a rast was considered positive when a serum bound > or = 1% of the total counts added. the ... | 1993 | 8448678 |
| serum-ige-facilitated allergen presentation in atopic disease. | the occurrence of facilitated ag presentation (fap) was investigated in atopic disease using an in vitro model in which the ability of cd23-expressing ebv-b cells was tested to present der p ii, a major allergen of housedust mite dermatophagoides pteronyssinus, to cells of autologous der p ii-specific cd4+ t lymphocyte clones. purified der p ii protein was immune complexed with ig by preincubation in sera from atopic patients containing der p ii-specific ige. incubation of ebv-b cells with these ... | 1993 | 8468493 |
| the effects of season, climate, and air-conditioning on the prevalence of dermatophagoides mite allergens in household dust. | clinical evidence reveals a strong relationship between dust mite allergen levels and asthma. this study suggests the relative importance and interactions among factors that influence mite allergen levels in human dwellings. | 1993 | 8473674 |
| allergenicity of the mite, blomia tropicalis. | blomia tropicalis (bt) occurs in a significant percentage of homes in tropical and subtropical geographic regions of the united states and europe and in countries in south america and asia along with the pyroglyphid mites, euroglyphus maynei (em), dermatophagoides pteronyssinus, and d. farinae. blomia species may be major sources of allergens in house dust in addition to dermatophagoides species and e. maynei. | 1993 | 8491936 |
| clonal analysis of human t-cell responses to fractionated house dust mite antigens (dermatophagoides pteronyssinus). | in the present study, we investigated the cellular basis of house dust mite-driven immune responsiveness in an atopic individual with perennial rhinitis. we established 40 human t-cell clones (cd3+, 4+, 8-) reactive to dermatophagoides pteronyssinus (dp) antigen under the restriction of hla-dr. by using the crude dp antigen and its 14 molecular weight (mw) fractions, we analyzed the distribution of t-cell-recognizing sites in the whole dp extract. we tested t-cell-mediated immunity through two p ... | 1993 | 8499778 |
| the effects of tumour necrosis factor alpha on mediator release from human lung. | although tumour necrosis factor alpha (tnf alpha) may be involved in the pathology of asthma, little is known about its role in mediator release from inflammatory cells in human lung. we investigated whether tnf alpha induced histamine release from mast cells in human chopped lung tissue and whether it modulated antigen-induced release of histamine and leukotrienes c4/d4/e4 from passively sensitized lung tissue. spontaneous histamine release in the presence of 1 nm tnf alpha for up to 4 h at 37 ... | 1995 | 8535096 |
| the maintenance of functional activity in human isolated bronchus after cryopreservation. | cryopreservation has been successfully used in the in vitro study of pharmacological responses of animal tissues and, to a limited extent, of human tissue. in this study, we examined the effect of cryopreservation on reactivity of human bronchus which was stored for a period of up to 3 weeks. thirty-two bronchial rings were obtained from each of four transplantation donors (four male, aged 32 +/- 15 years sd). eight rings from each patient were studied on day 0 (the day of retrieval) and an addi ... | 1995 | 8535098 |
| [comparison of intradermal and prick tests based on samples of the most common allergens in the zadar region]. | the practical clinical problem of evaluating the significance of positive dermal tests is present in daily in vivo diagnoses in allergology and clinical immunology. our research was aimed at a comparison of the two methods of dermal tests, namely, intradermal (i.d.) and prick tests, based on a sample of the most common allergenics in the zadar region. the intradermal method was applied to a group of 664 patients, the prick test was used in a group of 641 patients, and 60 patients were examined u ... | 1995 | 8538357 |
| distribution of dermatophagoides mite (acari: pyroglyphidae) antigens in homes of allergic patients in japan. | dust samples collected in 61 homes of patients with mite allergies and 11 homes of non-allergic people as controls were examined for antigen levels of dermatophagoides farinae hughes and dermatophagoides pteronyssinus (trouessart) to reveal the current distribution of allergenic mites in homes in japan. patient homes had higher antigen levels than control homes in comforters and pillows but not in futons, carpets, tatamis and wooden floors. samples with antigen levels of > or = 10 micrograms m-2 ... | 1995 | 8542786 |
| regulation and targeting of t-cell immune responses by ige and igg antibodies. | a set of chimeric antibodies with identical f(ab')2 fragments specific for the hapten 5-iodo-4-hydroxyl-3-nitrophenacetyl (nip), but with different human fc parts (gamma 1, gamma 2, gamma 3, gamma 4, epsilon), was used to compare the role of igg and ige antibodies in antigen presentation by human epstein-barr virus (ebv) b cells. two or three molecules of nip were coupled to one molecule of der pi (der pi-(3)nip), a major allergen of dermatophagoides pteronyssinus. both monomeric igg and perform ... | 1995 | 8550069 |
| [fauna and biology of the pyroglyphidae of poland]. | 5 valid species of pyroglyphid mites are actually known in polish fauna. it should be stressed that the knowledge of their occurrence in house-dust, bird nests and other habitats is still poor. the most common pyroglyphid house-dust mites, dermatophagoides pteronyssinus, d. farinae and euroglyphus maynei, were found in samples of dust from houses (warszawa, bydgoszcz, upper silesia), hospitals (katowice, sosnowiec), libraries (sosnowiec) and port buildings (gdynia). the both species of the genus ... | 1995 | 8553664 |
| human auto-anti-idiotypic antibodies to mite-specific ige can degranulate human basophils in vitro. | anti-idiotypic antibodies (anti-ids) to specific ige antibodies are formed spontaneously during an anti-allergen immune response and can be induced by immunotherapy. although anti-ids can down-regulate the production of ige antibodies, at least in experimental models, their possible role in the modulation of target cell reactivity remains ill-defined. | 1995 | 8556564 |
| comparative analysis of the genes encoding group 3 allergens from dermatophagoides pteronyssinus and dermatophagoides farinae. | group 3 allergens of the genus dermatophagoides represent one of the major groups of house dust mite allergens. the cdna sequence data for der p 3, in combination with both n-terminal amino acid sequences and substrate affinity data, have confirmed that the group 3 allergens are trypsin-like proteases. using the information from the der p 3 p3ws1 cdna clone, genes encoding both der f3 and der p 3 have now been amplified by the polymerase chain reaction and analysed. two der f3 clones and three d ... | 1996 | 8563488 |
| [natural allergens and recombinant allergens]. | since forty years, many allergens from different species responsible for allergies, have been purified and sometimes identified using classical methods of protein chemistry. for the first time in 1988, molecular biology technologies were applied to allergens, namely to a major allergen of the mite, dermatophagoides pteronyssinus. during the recent years many other allergens have been produced as recombinant proteins expressed in bacteria or yeast leading to the growing family of recombinant alle ... | 1995 | 8590581 |
| multicentre evaluation of capture assay radim liquid allergen for measurement of specific ige antibodies. | a multicentre trial of capture assay radim liquid allergen was performed to define the sensitivity, specificity and clinical reliability of the system in diagnostic allergology. the results of the evaluation were compared with clinical data and in vivo testing. good agreement was obtained for dermatophagoides pteronyssinus (d1), cat's epithelium (e1), betula verrucosa (t3) and olea europea (t9), artemisia vulgaris (w6) and parietaria officinalis (w19). some spreading of data was observed for art ... | 1995 | 8608200 |
| fine tuning of epitopic dominance induced by lung cancer on the igg response to bovine betalactoglobulin: towards a paraneoplastic immune marker. | investigating the humoral immune response to mucosal antigens in patients with lung cancer, we have documented a preferential immunoglobulin g (igg) binding to cryptic epitopes unmasked by the proteolysis of bovine beta-lactoglobulin (blg). in contrast, igg from healthy controls and patients with chronic bronchitis (copd) bind preferentially to continuous epitopes presented on both native (n) and denaturated (d) forms of this antigen. the present study further characterized the differences in th ... | 1996 | 8616757 |
| serum total and specific ige levels in children infected with human immunodeficiency virus. | human immunodeficiency virus (hiv) infection is associated with polyclonal b-cell activation, and elevated ige levels are found in infected adults. chronic nasal symptoms, sinusitis, and otitis media are particularly common in children infected with hiv. the possible allergic cause of nasal symptoms in children infected with hiv has not been explored. | 1996 | 8621856 |
| lung cancer-related changing profile of b-cell epitopes recognized on mucosal antigens: the der p1 model. | the natural immunoglobulin g (igg) response towards antigens presented at the mucosal level in patients with lung cancer, the most frequent mucosal malignancy in humans was studied. compared with healthy control subjects, patients with lung cancer display lower igg antibody titers toward antigen p1 of the house dust mite, dermatophagoides pteronyssinus (der p1), chronically presented to the respiratory mucosa. the present study further characterizes this defect in terms of antibody specificity. | 1995 | 8630893 |
| an in vivo model of allergic inflammation: pulmonary human cell infiltrate in allergen-challenged allergic hu-scid mice. | cb.17 severe combined immunodeficient (scid) mice were used to establish a model of allergic pulmonary inflammation. scid mice were intraperitoneally reconstituted with 10(7) peripheral blood mononuclear cells (pbmc) from patients sensitive to dermatophagoides pteronyssinus (dpt) and 2 weeks later were exposed to dpt aerosols. after dpt nebulization, scid mice engrafted with pbmc from dpt-sensitive patients developed a specific human ige response as well as an intense pulmonary infiltrate of hum ... | 1996 | 8647172 |
| comparative performance for immediate hypersensitivity skin testing using two skin prick test devices. | fifty-five patients were skin-tested by multi-test (m) and needle prick test (npt) to compare the reproducibility of the methods. we used 6 allergenic extracts: dermatophagoides pteronyssinus, dermatophagoides farinae, dog epithelium, cat pelt, american cockroach and mixed molds. a glycerosaline and a positive control (histamine 1 mg/ml) were performed in both methods. statistically significant differences in histamine and dermatophagoides farinae wheal reactions between the two methods (m > npt ... | 1995 | 8653226 |
| contact allergy to dermatophagoides in atopic dermatitis patients and healthy subjects. | to compare different house-dust-mite-derived allergenic materials and to correlate the presence of ige to dermatophagoides with patch test results, 313 atopic dermatitis (ad) patients and 100 healthy volunteers (hv) underwent patch tests with: dermatophagoides pteronyssinus (dpt) lyophilized purified alpha fraction in buffered saline/glycerol 50% and/or in petrolatum (bayropharm); 50% dpt and 50% dermatophagoides farinae (df) whole bodies in petrolatum and petrolatum oil (allergopharma-bracco); ... | 1995 | 8654075 |
| prevalence of sensitization to dermatophagoides pteronyssinus in several industrial populations. | skin tests with dermatophagoides pteronyssinus were carried out by the standard prick method in six groups of industrial workers: meat processing workers (n = 107), brewery workers (n = 96), animal food workers (n = 40), swine farmers (n = 32), paper-mill workers (n = 132) and wool-textile workers (n = 111). the control group consisted of 158 subjects who were tested during the preemployment examinations and had not worked in industrial plants before. skin reactions were read after 20 minutes by ... | 1995 | 8670010 |
| glutathione s-transferase a major allergen of the house dust mite, dermatophagoides pteronyssinus. | recently, we cloned a new dermatophagoides pteronyssinus allergen homologous with glutathione s-transferase. ige radioimmunoassays against the escherichia coli lysate of the recombinant clone reveal that 40% of mite allergic subjects recognize the mite glutathione s-transferase allergen. of these sera, greater that 50% have reactivity with the recombinant mite glutathione s-transferase that is greater than 10 times the result observed with a normal control. immunoblotting studies with sera from ... | 1995 | 8719107 |
| epitope analysis of hla-dr-restricted helper t-cell responses to der p ii, a major allergen molecule of dermatophagoides pteronyssinus. | t-cell epitopes of der p ii, a major allergen of dermatophagoides pteronyssinus, were analyzed by using human t-cell clones. we tested 38 cloned t cells from two japanese patients with allergic rhinitis, and identified at least two peptides (k33-t47 and i58-c73) as helper t-cell epitopes. the former epitope was shown to be restricted by hla-drb1*1502, and the latter by hla-drb1*0405, both of which are typical japanese hla-dr alleles, suggesting that those t-cell epitopes might be important for t ... | 1996 | 8721525 |
| immunologic responses following respiratory sensitization to house dust mite allergens in mice. | allergens from the house dust mite dermatophagoides pteronyssinus are a major cause of human respiratory diseases, including asthma. in order to help in understanding the early events in allergen sensitization, a murine model of allergic respiratory disease was developed. mice were immunized by intranasal inoculation of der p 1 or der p 2 on days 0, 3, 7, 10, 14, 17, 21 and 29. t cell reactivity was determined using in vitro assays of allergen-specific cytokine production by cells from the splee ... | 1996 | 8724006 |
| [risk indicators of reaginic allergy: relations between atopic dermatitis and serum specific ige of various pneumallergens]. | this study was conducted in 1,091 children and adolescents submitted to an allergy check-up for respiratory symptoms. all subjects had a research of ige for dermatophagoides pteronyssinus, cat and timothy grass pollen by cap-system pharmacia. three hundred subjects had a personal history of atopic dermatitis. they were compared to the 791 others. no difference was found for age, sex, family history of allergic respiratory disease, breast feeding nor exposure to tobacco smoke. the ige level was s ... | 1995 | 8734080 |
| [allergenic mites found in raw material of herbs based on literature about the pyroglyphidae (acari: acaridida)]. | the occurrence of two pyroglyphid mites. euroglyphus maynei (cooreman, 1950) and dermatophagoides pteronyssinus (trouessart, 1897) in the raw material of herbs is discussed and compared with the literature data. these mites are considered to be the cause of human atopic diseases. | 1996 | 8755152 |
| allergen-specific ige levels and mite allergen exposure in children with acute asthma first seen in an emergency department and in nonasthmatic control subjects. | sensitization to allergens has been shown to be a risk factor for adults with acute asthma first seen in the emergency department. | 1996 | 8757201 |
| absence of continuous epitopes in the house dust mite major allergens der p i from dermatophagoides pteronyssinus and der f i from dermatophagoides farinae. | the house dust mite has been shown to be an important source of domestic allergens associated with immediate hypersensitivities. the group i mite allergens der p i from dermatophagoides pteronyssinus and der f i from d. farinae display extensive amino acid sequence homology and have similarities with cysteine protease enzymes. | 1996 | 8789541 |
| monoclonal antibodies against dermatophagoides group i allergens as pseudo-cystatins blocking the catalytic site of cysteine proteinases. | the enzyme allergens der p i and der f i produced by the house dust mites dermatophagoides pteronyssinus and d. farinae display partial sequence homology with other members of the cysteine proteinase superfamily. we report that certain widely used mouse mabs against these group i allergens indeed crossreact with the plant enzymes papain, bromelain and ficin. the recognition sites of these anti group i mabs comprise conformational and thermolabile epitopes involved in molding the catalytic center ... | 1996 | 8803616 |
| eosinophil locomotion and the release of il-3 and il-5 by allergen-stimulated mononuclear cells are effectively downregulated in vitro by budesonide. | treatment of allergic asthma with inhaled corticosteroids, such as budesonide (bdn), results in downregulation of t-cell activation and of eosinophil recruitment. | 1996 | 8809423 |
| the significance of reactions to purified fractions of dermatophagoides pteronyssinus and dermatophagoides farinae in canine atopic dermatitis. | the significance of reactions to crude extracts and purified fractions of the house dust mites dermatophagoides pteronyssinus (der p i and der p ii) and dermatophagoides farinae (der f i and der f ii) was evaluated in dogs with clinical manifestations of atopic dermatitis (ad). in 13 healthy control dogs and eight dogs with ad, immediate skin test reactivity was determined to serial dilutions of der p i, der p ii, der f i and der f ii. in addition, allergen-specific iggd antibodies were determin ... | 1996 | 8809997 |
| the prevalence of atopic sensitization and respiratory symptoms among estonian schoolchildren. | to assess the prevalence of atopic sensitization, asthma and other respiratory disorders among estonian schoolchildren in a coastal industrial city and an inland university town, i.e. tallinn and tartu. | 1995 | 8821300 |
| month of birth, atopic disease, and sensitization to common aeroallergens in greece. | the aim of the study was to evaluate the influence of the month of birth on the development of atopic disease and the rate of sensitization to common environmental allergens in greece. analysis of the month of birth of 1755 individuals with atopic disease (asthma, rhinitis, atopic dermatitis), out of whom 771 had positive skin tests/rast to dermatophagoides pteronyssinus and mixed grasses, in comparison to total live births (2,877,733) in greece during 1968-88, was performed and showed significa ... | 1995 | 8822396 |
| skin prick tests with standardized extracts of inhalant allergens in 7099 adult patients with asthma or rhinitis: cross-sensitizations and relationships to age, sex, month of birth and year of testing. | the results of skin prick tests (spts) performed between 1981 and 1992 on 7099 adult patients with asthma and/or rhinitis were retrospectively analyzed. standardized soluprick extracts of dermatophagoides pteronyssinus, animals and pollen, and unstandardized extracts of cladosporium++ and chironomids (red mosquito larvae, chir), were used. the proportion of atopics (patients with positive spt results) was 44 percent, decreasing from 61 percent in patients 14-20 years old to 18 percent in patient ... | 1996 | 8833167 |
| ige-mediated basophil releasability is influenced by intrinsic factors and by ige on the cell surface. | experiments were done to clarify the mechanisms associated with releasability of histamine. first, washed leukocytes from 23 asthmatic patients sensitive to mite allergen were challenged with der p 1, a major allergen isolated from dermatophagoides pteronyssinus, or anti-ige. a significant correlation was observed between the ratio of der p 1-specific ige titer to total ige level (s/t) in the patient's plasma and either the reactivity (maximal percentage of histamine release; rs = 0.514, p = 0.0 ... | 1995 | 8834823 |
| receiver-operating characteristic (roc) curves: a fundamental tool for improving the clinical usefulness of in vitro ige tests. | in order to establish the most efficient thresholds for serum allergen-specific ige measured by the pharmacia cap system, a "second-generation" in vitro method, we evaluated results from 89 subjects with suspected inhalant allergies, using receiver-operating characteristic (roc) curve analysis. sera samples were analyzed by the pharmacia cap system for specific ige, the choice of allergens to be tested being based upon the symptoms and clinical history of each patient. results were analyzed by r ... | 1996 | 8837664 |
| sensitization to dermatophagoides siboney, blomia tropicalis, and other domestic mites in asthmatic patients. | mite species adapted to warm, humid climates are commonly found in house dust in the tropics. in cuba, dermatophagoides pteronyssinus, d. siboney, and blomia tropicalis are the most common and abundant mite species in house dust. to investigate the pattern of sensitization of cuban asthmatic patients to common mite species, we skin-prick-tested (spt) 148 patients with a clinical history of asthma and possible mite allergy, and determined specific ige antibodies against mite allergens (d. pterony ... | 1996 | 8863927 |
| transient sensitization to house-dust mites: a study on the influence of mite exposure and sex. | this study aimed to evaluate the prognosis of a previous positive skin test to house-dust mites (hdm) in relation to environmental exposure. a total of 115 children, 50 from stockholm and 65 from northern sweden, all with a previous (average 2.5 years) positive phazet (pharmacia ab, uppsala, sweden) skin prick test (spt) to extracts of dermatophagoides pteronyssinus (dpt) and/or d. farinae (df) were included. dust samples were collected from the children's mattresses, and the total (dpt, df, and ... | 1996 | 8863929 |
| determination of the stability of diluted allergen extracts using a concentration step prior to east inhibition. | generally the stability of diluted allergen extracts, as used for skin testing, provocation testing and immunotherapy can not be measured using a normal enzyme allergosorbent test (east) inhibition method. | 1996 | 8877162 |
| respiratory allergy to parietaria pollen in 348 subjects. | we have revisited 348 records of allergic outpatients to parietaria, ratio m/f 1:1 and mean age 29.46 +/- 12.49 yrs. 97 of them were skin positive to parietaria only (a-group: m/f 37/60); 251 patients were skin positive also to other allergens (b-group: m/f 137/114). mean age of a-group patients (35.41 +/- 12.91) was higher than b-group (27.16 +/- 11.54; p < 001). dividing mono and polysensitized subjects by age decade, a different distribution was found between the two groups (p < 001): monosen ... | 1996 | 8882753 |
| childhood asthma and exposure to indoor allergens: low mite levels are associated with sensitivity. | the prevalence and level of sensitivity to indoor allergens were studied in relation to current exposure at home in 124 children with perennial asthma living in three climatic zones of sweden. the house dust mite (hdm) allergen levels were higher in the south than in the north (p < 0.001), while cat and dog allergen levels tended to be higher in the north than the south (n.s.). thirty-four percent of the children were sensitive to the hdm dermatophagoides pteronyssinus, as determined by ige anti ... | 1996 | 8902855 |
| the disturbance of epidermal barrier function in atopy patch test reactions in atopic eczema. | atopic eczema (ae) is a common skin disorder. eczematous lesions showing macroscopic, microscopic and immunopathological resemblance to lesional ae can be induced by aeroallergens by epicutaneous testing (atopy patch test, apt). altered epidermal barrier function, as determined by transepidermal water loss (tewl), is a typical feature of patients with ae. the present investigation was performed to define the differences in the epidermal barrier function between positive apt reactions to aeroalle ... | 1996 | 8915146 |
| interaction of human lung surfactant proteins a and d with mite (dermatophagoides pteronyssinus) allergens. | human lung surfactant proteins a (sp-a) and d (sp-d) are both collagenous c-type lectins which appear to mediate antimicrobial activity by binding to carbohydrates on micro-organisms and to receptors on phagocytic cells. purified native sp-a and sp-d, isolated from human bronchoalveolar lavage fluid, were found to bind to whole mite extracts (dermatophagoides pteronyssinus) and the purified allergen der p i, in a carbohydrate-specific and calcium-dependent manner. binding was inhibited by ethyle ... | 1996 | 8918587 |
| occupational allergy due to spider mites: tetranychus urticae (koch) and panonychus citri (koch). | allergy to both house dust and storage mites is well established, but information about other species of mites is scant. | 1996 | 8955575 |
| mite sensitivity and exposure in the city of santa fe, argentina. | little information is available on mite allergen levels and rates of sensitization to domestic mites in south america. the purpose of this study was to establish the rate of sensitization to domestic mites in asthmatics and measure mite allergen levels in pillows and mattresses of mite allergic individuals in santa fe, argentina. dust samples were collected from the pillows and mattresses of 56 asthmatics (24 females and 32 males, mean age 17.6 +/- 11.5 years), who had been previously skin teste ... | 1996 | 8959537 |