| protoplast formation and yeast cell-wall structure. the action of the enzymes of the snail, helix pomatia. | 1. the digestive juice of the snail helix pomatia was used in the study of the degradation of isolated cell-wall preparations from a strain of saccharomyces carlsbergensis. 2. the crude enzyme system was fractionated by gel filtration and the activities of the more specific fractions thus obtained were examined. 3. results are discussed with respect to (a) the nature of various factors that are essential for protoplast formation and cell-wall dissolution and (b) structures envisaged in yeast cel ... | 1966 | 5964965 |
| studies on glycoproteins. xv. substrate-dependence of o-seryl-n-acetylgalactosaminide glycosidase (helix pomatia) activity and mechanism of the enzymatic reaction. | | 1967 | 6077032 |
| a double antibody radioimmunoassay for free and conjugated estradiol-17 beta in cow's milk. | a radioimmunoassay for free estradiol-17 beta, conjugated estradiol-17 beta or total (free + conjugated) estradiol-17 beta in defatted milk of cows is described. conjugated estradiol-17 beta was hydrolyzed by enzymes of helix pomatia juice. estrogens were extracted with dichloromethane; no other purification step was required before radioimmunoassay because of the high specificity of the antiserum. immunoprecipitation was used to separate bound and free estradiol-17 beta. concentrations measured ... | 1984 | 6100343 |
| induction of tryptophan oxygenase and tyrosine aminotransferase by metabolites of hydrocortisone. | metabolites of hydrocortisone were isolated from rat liver on a preparative scale, fractionated by column chromatography on sephadex lh-20 and silica gel and tested for biological activity. apart from the well known neutral metabolites, steroid glucuronides and sulfates, we obtained metabolite fractions containing non-conjugated steroidal carboxy acids and acid metabolites of unknown structure. one of these fractions induced tyrosine aminotransferase (ec 2.6.1.5) in adrenalectomized female rats ... | 1980 | 6111341 |
| n-hydroxyphenacetin, a new urinary metabolite of phenacetin in the rat. | n-hydroxyphenacetin has been found in the urine of rats dosed with phenacetin, extending previous reports that phenacetin is n-hydroxylated by liver microsomes in vitro. after an oral dose of phenacetin (500 mg/kg) urine was collected for 24 hr, conjugates hydrolyzed with extract of helix pomatia, and the metabolites extracted with dichloromethane and treated with diazomethane. methylation of n-hydroxyphenacetin produced a stable derivative, n-methoxyphenacetin, which was separated from most oth ... | 1981 | 6113936 |
| different expression of lyt differentiation antigens and cell surface glycoproteins by a murine t lymphoma line and its highly metastatic variant. | cloned lines of the methylcholanthrene-induced dba/2 t lymphoma eb and its highly metastatic variant line esb were analyzed for differences in the expression of serologically detectable cell surface differentiation markers. flow cytofluorographic analysis of cells stained with fluorescein isothiocyanate-conjugated monoclonal rate anti-mouse thy-1, lyt-1, lyt-2 and complement-dependent cytotoxicity with mouse alloantisera against lyt-3.2 and ly-6.2 revealed, for the parental low metastasizing lin ... | 1982 | 6124426 |
| identification of major sulfate conjugates in the metabolism of propranolol in dog and man. | a large portion of the dose of propranolol in animals and man is unaccounted for. using radiotracer and hplc techniques, five previously unrecognized polar and labile metabolites were found in dog urine, together accounting for 34% of the urinary radioactivity. the two main metabolites, peak 2 (7% of the radioactivity) and peak 4 (17%) could be isolated and purified by butanol extraction and reversed phase hplc. direct probe ms analysis of the main peak 4 and gc/ms analysis of the same peak afte ... | 1983 | 6137341 |
| spontaneous redistribution of cell-surface glycoproteins in lymphoid cells during cytokinesis. | the 'unperturbed' distribution of plasma membrane glycoproteins during cytokinesis has been examined by immunofluorescence and electron microscopy on dividing mouse and rat lymphoid cells fixed before being labelled with the appropriate reagents. two groups of molecules which cap 'spontaneously' to the uropod of non-dividing cells, i.e., the common receptors for helix pomatia (hpa) and peanut agglutinin (pna) (and in particular the thymocyte glycophorin-like glycoprotein) and membrane immunoglob ... | 1984 | 6148239 |
| defective glycoprotein c of the syncytial strain of herpes simplex virus type 1. | glycoproteins of the herpes simplex virus type 1 (hsv-1) non-syncytial strain kupka showed two times higher affinity for the helix pomatia (hp) lectin than glycoproteins of the syncytial strain hszp. as revealed by the results of immunoprecipitation test and by the comparison of the synthesis of virus glycoproteins, glycoprotein c (gc) was synthesized in sufficient amounts in the hszp strain-infected cells. the difference in the binding to hp lectin was found to be caused by decreased affinity o ... | 1984 | 6152123 |
| isolation, characterization and implications of anti-tf (thomsen-friedenreich) agglutinins from different sources. | anti-tf agglutinins from peanut (arachis hypogaea) and from vertebrate sera of different species have been successfully isolated by affinity chromatography on acid-activated sepharose 4 b. the proteins were characterized by immunoelectrophoresis, polyacrylamide gel electrophoresis in the presence of sds and with respect to their carbohydrate binding specificities. anti-tf substances from sera showed one precipitin arc in immunoelectrophoresis, but quantitative immunoprecipitation revealed our hu ... | 1980 | 6157637 |
| clonal expression of the tn antigen in erythroid and granulocyte colonies and its application to determination of the clonality of the human megakaryocyte colony assay. | to evaluate whether exposure of tn determinants at the surface of human erythrocytes, platelets, and granulocytes could arise from a somatic mutation in a hemopoietic stem cell, burst-forming unit erythroid (bfu-e) colonies, colony-forming unit granulocyte-macrophage (cfu-gm), and colony-forming unit-eosinophil (cfu-eo) were grown from a blood group o patient with a typical tn syndrome displaying two distinct populations (tn(+) and tn(-)) of platelets, granulocytes, and erythrocytes. a large num ... | 1982 | 6175663 |
| natural and antibody-dependent killer cells in the thymus. | thymocytes contain a small population of light (o = 1.067), large cells with high cytolytic potential against natural killer target as well as against igg- or igm-coated erythrocytes and nucleated cells, thus displaying natural killer and antibody-dependent cellular cytotoxicity functions, but not exhibiting t killer cell capacity. as far as characterized, the cells show t cell characteristics: they are nonadherent, nonphagocytic, surface immunoglobulin-negative, c3 receptor-negative, partly fc ... | 1982 | 6185347 |
| identical lectin binding patterns of human melanocytes and melanoma cells in vitro. | cell surface glycoconjugate patterns of human epidermal cells and of melanoma cells (mc) in primary culture derived from 11 primary and metastatic melanomas were investigated using fluorescent and horseradish peroxidase conjugated lectins for visualization at the light and electron microscopic level. the lectin labeling profiles of human melanocytes (m) and mc were found to be identical. according to their binding patterns, the lectins tested were grouped into three categories: (1) lectins bindi ... | 1983 | 6187869 |
| application of lectin--gold complexes for electron microscopic localization of glycoconjugates on thin sections. | a method is described for the electron microscopic detection of lectin-binding sites in different cellular compartments and extracellular structures that uses thin sections from resin-embedded tissues. various lectins (ricinus communis lectin i and ii, peanut lectin, lotus tetragonolobus lectin, ulex europeus lectin i, lens culinaris lectin, helix pomatia lectin, and soybean lectin) were bound to particles of colloidal gold and used for direct staining of thin sections or glycoprotein--gold comp ... | 1983 | 6190857 |
| an abnormal fibrinogen (copenhagen ii) with increased sialic acid content associated with thrombotic tendency and normal liver function. | an increased sialic acid content of the fibrinogen molecule is found in foetal fibrinogen and as an acquired disorder in hepatic disease. a qualitatively abnormal fibrinogen was detected in the plasma of a 25-year-old man with a thrombotic tendency. the purified fibrinogen had a significantly increased content of sialic acid, an abnormal fibrin monomer polymerization, and a changed mobility in crossed affinity-immunoelectrophoresis using immobilized helix pomatia lectin. the patient had no bioch ... | 1984 | 6205441 |
| c3 receptors on human lymphocyte subsets and recruitment of adcc effector cells by c3 fragments. | the presence of c3 receptors on human peripheral blood lymphocytes (pbl) and on the adcc-exhibiting subset (k cells) thereof was analyzed by rosetting with bovine erythrocytes (eb) or chicken erythrocytes (ec) carrying human c3b, c3bi, or c3d. the indicator cells were coated with 20,000 to 100,000 c3 fragments, obtained by c3 activation with purified proteins of the alternative pathway and trypsin treatment. adcc was studied at the cellular level by means of a plaque assay, with complement-free ... | 1983 | 6222118 |
| helper function of human t cells with different affinity for helix pomatia a hemagglutinin in a tetanus toxoid-induced b cell differentiation system. | t cells from human peripheral blood were enriched in t4+ cells by lysis of t8+ cells with the monoclonal antibody okt8 plus complement. the t4+ subset was separated into 4 fractions differing in avidity for the lectin helix pomatia a hemagglutinin (hp). the fractions were studied for their capacity to help autologous b cells to differentiate and mature into immunoglobulin (ig) synthesis and secretion after activation with tetanus toxoid (tt) in vitro. to ensure the antigen specificity of inducti ... | 1984 | 6238830 |
| unusual lectin-binding properties of a herpes simplex virus type 1-specific glycoprotein. | lysates from herpes simplex virus type 1-infected cells were subjected to affinity chromatography on soybean and helix pomatia lectins. one of the virus-specified glycoproteins, probably the herpes simplex virus type 1-specific gc glycoprotein, bound to the lectins and was eluted with n-acetylgalactosamine. the affinity chromatography permitted a high degree of purification of the type-specific glycoprotein with respect to both host cell components and other viral glycoproteins. the lectin affin ... | 1981 | 6264128 |
| [a simple method for radioimmunoassay of total estrogen in urine]. | a simple and rapid radioimmunoassay (ria) without extraction or purification was developed for total estrogen (conjugated and unconjugated estrogen) in urine from normal men and nonpregnant women. antiserum used to ria was produced by immunizing rabbits with estriol-16 alpha-glucuronide (e3-16-g)-bsa conjugates. antiserum to e3-16-g.bsa significantly cross-reacted with e1 (100%), e2 (100%), e3 (100%), e3-16-g (100%), and e3-17-g (100%) and did not react with other conjugated estrogen in urine. u ... | 1983 | 6299812 |
| regional distribution of n-acetyl-d-galactosamine residues in the glycocalyx of glomerular podocytes. | helix pomatia lectin (hpl) bound to colloidal gold was used as a specific cytochemical probe for the localization of terminal nonreducing n-acetyl-d-galactosamine residues in thin sections of rat kidney. in the glomerulus, lectin-binding sites were associated only with the podocyte foot process bases and were not found on the free cell surface of podocytes or on any other glomerular components. gold-particle label was often arranged in the form of clusters which extended from the foot process ba ... | 1983 | 6302101 |
| populations of herpes simplex virus glycoprotein gc with and without affinity for the n-acetyl-galactosamine specific lectin of helix pomatia. | two fractions of herpes simplex virus glycoprotein gc were isolated and characterized by means of immunosorbent-purification with monoclonal antibodies against gc and helix pomatia lectin (hpa) affinity chromatography. about 25 per cent of the glycoprotein gc population demonstrated affinity for the lectin, compatible with presence of n-acetylgalactosamine as terminal sugar of the oligosaccharide. the hpa-binding populations of gc appeared as two electrophoretic bands with lower molecular weight ... | 1983 | 6305311 |
| solubilization and partial purification of putative calcium channels labelled with [3h]-nimodipine. | high-affinity binding sites for the potent 1,4-dihydropyridine calcium channel blocker [3h]-nimodipine were solubilized from guinea-pig skeletal muscle microsomes with digitonin and chaps [3-(3-cholamidopropyl)-dimethyl-ammonio-l-propanesulfonate]. detergent-solubilized binding sites could not be sedimented by centrifugation (50,000 x g, 4 h), passed freely through 0.2 micron nitrocellulose filters and were stable at 4 degrees c with half-lives of greater than 60 h. the solubilized 1,4-dihydropy ... | 1983 | 6314149 |
| glycoprotein c of herpes simplex virus type 1: characterization of o-linked oligosaccharides. | in contrast to other viral glycoproteins, the herpes simplex virus (hsv) glycoprotein c(gc) binds to the n-acetylgalactosamine-specific helix pomatia lectin (hpa). in the present paper gc was purified by affinity chromatography with monospecific antibodies and the purified glycoprotein was subjected to protease digestion. hpa-binding protease-resistant glycopeptides were isolated by lectin affinity chromatography. the isolated structures did not bind to concanavalin a and seemed to lack charged ... | 1983 | 6319556 |
| herpes simplex virus type-selective enzyme-linked immunosorbent assay with helix pomatia lectin-purified antigens. | helix pomatia lectin-purified antigens with specific reactivity to herpes simplex virus type 1 (hsv-1) and hsv-2 antibodies in human sera were used in an enzyme-linked immunosorbent assay. the type specificity of the antigens was assessed by double immunodiffusion precipitation in gel against rabbit hsv-1 and hsv-2 hyperimmune sera, and by enzyme-linked immunosorbent assay with human reference sera containing antibodies to either type of hsv. fifty-two sera from patients with documented infectio ... | 1984 | 6321548 |
| intracellular protein kinase and calcium inward currents in perfused neurones of the snail helix pomatia. | changes in the amplitude of the calcium inward current caused by intracellular administration of tolbutamide (an inhibitor of the cyclic amp-dependent protein kinase activity) or catalytic subunits of camp-dependent protein kinases from rabbit myocardium were studied on internally perfused nerve cells of the snail, helix pomatia. intracellular administration of 7 mm tolbutamide caused a rapid decline of the amplitude of the calcium current that had been stabilized by theophylline; the effect was ... | 1984 | 6324030 |
| phenotypic and cytogenetic characteristics of a new epstein-barr virus negative cell line (skw 4) derived from a b-cell lymphoma. | a new epstein-barr virus nuclear antigen (ebna) negative cell line skw 4 has been established in vitro from a patient with diffuse histiocytic lymphoma. the skw 4 seems to be an authentic human tumour cell line as evidenced by its ebv negativity, monoclonality and aneuploidy tested during early in vitro passage. the cell line expresses surface mu and kappa-chains, hla-dr antigen, c3 and fc receptors and b-cell lineage antigens. the karyotypic analyses demonstrated many numerical and structural a ... | 1983 | 6329938 |
| determination of herpes simplex virus type-specific antibodies by solid-phase ria on helix pomatia lectin-purified antigens. | herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) antibodies in humans and animals were determined by a micro-solid-phase radioimmunoassay with helix pomatia lectin/sepharose 4b-purified antigens from hsv-1- and hsv-2-infected cells. a low but constant heterotypic reactivity was found with hsv-1 antigen, whereas hsv-2 antigen was type specific, as evidenced by (1) its reactivity with sera from hsv-2- but not with those from hsv-1-immunized animals, (2) its reactivity with monoclonal antibod ... | 1984 | 6330240 |
| demonstration of t lymphocytes in human blood and cerebrospinal fluid with helix pomatia a hemagglutinin. | peroxidase-labelled helix pomatia a hemagglutinin (hph) was used as a t-cell marker for neuraminidase-treated human lymphocytes from blood or cerebrospinal fluid (csf). lymphocytes from the blood of 22 patients with noninflammatory diseases of the central nervous system and from the csf of 16 patients with noninfectious diseases and 29 patients suffering from meningitis or meningoencephalitis were studied. most hph-binding cells were found in normal csf. the variance in the number of reactive ly ... | 1984 | 6331702 |
| phaseolus vulgaris phytohaemagglutinin (pha) binds to the human t lymphocyte antigen receptor. | the interaction of phytohaemagglutinin (pha) with the human t lymphocyte antigen receptor (ti) was explored. nonidet-p40 lysates of surface-labelled hpb-all cells were immunoprecipitated with pha, using a rabbit anti-(pha)-serum, as well as clonotypic monoclonal antibodies (h1-2d4 and t40/25) and a rabbit antiserum (r-43) against ti. one- and two-dimensional sds-polyacrylamide electrophoresis under reducing and non-reducing conditions showed that both the clonotypic antibodies and pha precipitat ... | 1984 | 6335429 |
| fractionation of human lymphocytes on helix pomatia a hemagglutinin-sepharose and wheat germ agglutinin-sepharose. | | 1984 | 6335548 |
| sheep peripheral blood-t-lymphocytes: isolation, separation and surface receptors for helix pomatia agglutinin and peanut agglutinin from arachis hypogaea. | | 1984 | 6335945 |
| detection and quantitation of 3 alpha-hydroxy-1-methylen-5 alpha-androstan-17-one, the major urinary metabolite of methenolone acetate (primobolan) by isotope dilution--mass spectrometry. | a highly accurate method has been developed for detection and quantitation of 3 alpha-hydroxy-1-methylen-5 alpha-androstan-17-one, the major urinary metabolite of methenolone acetate (primobolan) in man. unlabelled as well as 2h-labelled 3 alpha-hydroxy-1-methylen-5 alpha-androstan-17-one were synthesized from 1-methylen-5 alpha-androstane-3,17-dione. a fixed amount of the internal standard was added to a fixed amount of urine and the mixture was treated with helix pomatia for 24 h. after extrac ... | 1983 | 6339822 |
| evaluation of labelling methods for bovine t and b lymphocytes. | two lectins, one from helix pomatia (hp) and one from peanut (pn), were evaluated as bovine t cell markers. hp attaches to about 40% of the bovine peripheral blood lymphocytes (pbl). with an indirect technique about 60% of pbl are hp positive, while pn attaches to a slightly higher proportion of the pbl. in double labelling experiments, hp was shown to bind only to ig negative cells while 2% of the pbl were double labelled with pn and anti-ig. the influence of different pretreatments of the pbl ... | 1983 | 6346664 |
| relationship between mouse lymphocyte receptors for peanut agglutinin (pna) and helix pomatia agglutinin (hpa). | the relationship between mouse lymphocyte receptors for peanut agglutinin (pna) and helix pomatia agglutinin (hpa) has been investigated by immunofluorescence (cocapping) and radiolabeling. in neuraminidase-treated and untreated thymocytes there are two groups of glycoproteins which bind roughly equivalent amounts of pna. one group also carries all the detectable receptors for hpa, the other binds only pna. binding inhibition experiments suggest that pna and hpa receptors are in close proximity ... | 1983 | 6357809 |
| fetal forms of oligoaminopeptidase, dipeptidylaminopeptidase iv, and sucrase in human intestine and meconium. | particles of meconium sedimenting at 105,000 g contain sucrase and various brush border peptidase activities. oligoaminopeptidase, dipeptidylaminopeptidase, and sucrase solubilized by papain from meconium particles of preterm newborns or from brush border of human fetuses during the 4th month of gestation were compared with the same enzymes prepared from adult jejunal and ileal brush border. the following are characteristics of fetal intestinal brush border enzymes: (a) a faster anodal electroph ... | 1984 | 6363665 |
| distinct cytoskeletal domains revealed in sperm cells. | antibodies against different cytoskeletal proteins were used to study the cytoskeletal organization of human spermatozoa. a positive staining with actin antibodies was seen in both the acrosomal cap region and the principal piece region of the tail. however, no staining was obtained with nitrobenzoxadiazol-phallacidin, suggesting that most of the actin was in the nonpolymerized form. most of the myosin immunoreactivity was confirmed to a narrow band in the neck region of spermatozoa. tubulin was ... | 1984 | 6381503 |
| antigen-induced plaque-forming cell responses in cultures of peripheral blood mononuclear cells of human neonates and infants. | human cord blood mononuclear cells (cbmc) were stimulated in vitro with a number of t cell-dependent antigens. antigen-induced b cell activation was measured applying a plaque-forming cell assay for the detection of antigen-specific igm-secreting b cells. with the exception of diphtheria toxoid, the antigens ovalbumin, sheep red blood cells, helix pomatia hemocyanin, burro red blood cells, and tetanus toxoid elicited an igm-plaque-forming cell response in cultures of cbmc to levels obtained for ... | 1984 | 6389806 |
| lectin-binding spectra in the hyperplastic human tonsil. effect of formalin fixation and paraffin embedding on lectin affinity of tissue components. | in order to determine the effect of routine fixation on the lectin affinity of tissue structures, we used unconjugated lectins and an indirect immunoalkaline-phosphatase method for frozen sections, and the peroxidase-anti-peroxidase method for paraffin-embedded, formalin-fixed tissue sections. fourteen hyperplastic human tonsils were used, and the results of the binding spectra of each lectin were compared. in general, the binding spectrum detected in the paraffin sections was part of the broade ... | 1984 | 6396285 |
| different t cell antigens and receptors for peanut agglutinin and helix pomatia agglutinin on steroid-sensitive and resistant lymphocytes in the rabbit using double immunofluorescence. | rabbit t lymphocytes were characterized using fluorochrome-labelled antisera against thymus cell determinants and fluorochrome-labelled lectins. three anti-t cell antisera were used, detecting different antigenic determinants on the majority of t cells. only small subpopulations were found stained by one of the three antisera. after dexamethasone (dx) treatment, the proportion of t cells was significantly increased in bone marrow and appendix, presumably by different mechanisms. cells binding pe ... | 1983 | 6409802 |
| in situ-differentiation of lymphocytes in fixed tissues with peroxidase-labeled helix pomatia lectin: revelation of the helix pomatia lectin receptor on cell surfaces. application in lymph node tissues and cutaneous lymphomas. | a receptor for helix pomatia lectin (hpl) occurs on 71.5% of peripheral blood lymphocytes. with double incubation techniques we could show that the population of hpl-positive lymphocytes is by far (greater than 90%) identical with the population of t lymphocytes, as determined by "classical" markers (e rosetting) and newly developed t cell markers like okt3 or anti-human lyt3 monoclonal antibodies. in formaldehyde-fixed lymph node sections we could demonstrate the homing area of hpl-positive lym ... | 1983 | 6412296 |
| differentiation of bacillus anthracis and other bacillus species by lectins. | bacillus anthracis was agglutinated by several lectins, including those from griffonia simplicifolia, glycine max, abrus precatorius, and ricinus communis. some strains of bacillus cereus var. mycoides (b. mycoides) were strongly reactive with the lectin from helix pomatia and weakly reactive with the g. max lectin. the differential interactions between bacillus species and lectins afforded a means of distinguishing b. anthracis from other bacilli. b. cereus strains exhibited heterogeneity with ... | 1984 | 6418761 |
| [ultrastructural detection of the heterogeneity of glycocalyx in convoluted and straight proximal tubules of rat kidney by the lectin-gold complex technic]. | a new histochemical technique in which lectin-gold complexes are applied directly to thin and semi-thin sections of tissue was used to determine the distribution of helix pomatia lectin binding sites in the proximal tubule of rat kidney. whereas a strong labeling was seen on the brush border and baso-lateral plasma membranes of convoluted tubules, the plasma membrane of cells from the medullary straight segment was unlabeled. some profiles at the frontier between cortex and medulla had a mosaic ... | 1983 | 6419990 |
| detection of blood group a-like substance in bacterial and viral vaccines by countercurrent immunoelectrophoresis using helix pomatia lectin. | the technique of countercurrent immunoelectrophoresis (ci), using the n-acetyl glucosamine-binding lectin from helix pomatia, provided a rapid, sensitive, inexpensive, specific and reliable method for assaying blood group a-like substances in both bacterial and viral vaccines. blood group a-like substance was detected in the pneumococcal polysaccharide vaccine manufactured by merck sharp & dohme up to 1981 and in a staphylococcus vaccine ( staphage lysate) manufactured by delmont laboratories. o ... | 1984 | 6429147 |
| hla phenotype and insulin antibody production. | hla phenotypes have been determined in 79 patients as part of a prospective study of factors governing the immune response to injected insulin. igg insulin antibody levels 6 months after starting treatment with bovine insulin were significantly higher in patients bearing hla-dr7 and this in conjunction with the lack of a similar pattern in the igg response to helix pomatia haemocyanin, suggests the presence of an immune response gene for insulin. the hyporesponsiveness of hla-b8/dr3/c4aq0 positi ... | 1984 | 6432385 |
| appearance of helix pomatia lectin-binding sites on podocyte plasma membrane during glomerular differentiation. a quantitative analysis using the lectin-gold technique. | the visceral glomerular epithelial cells (podocytes) of newborn rat kidneys were examined by the lectin-gold technique to determine the distribution of helix pomatia lectin-binding sites during glomerular development. previous work has shown that this lectin, which primarily recognizes terminal n-acetyl-d-galactosamine residues of glycoconjugates (but also n-acetyl-d-glucosamine to a lesser extent), specifically labels the plasma membrane glycocalyx of podocyte foot process bases, whereas the pl ... | 1984 | 6471810 |
| binding of lectins to human mammary tumors: ultrastructural study. | the site of several lectin receptors in human mammary tumors and stroma was studied with the electron microscope. the advantage of the ultrastructural over light microscopic study was that lectin receptors could be localized with precision on the different cell types of the tumor and stroma. eighteen human mammary carcinomas were incubated with three peroxidase-labeled lectins: peanut agglutinin (pna), helix pomatia, and ulex europaeus i (uea). these lectins reacted in a selective way; some tumo ... | 1984 | 6487820 |
| enhanced metabolism of [2,4,6,7-3h] estradiol-17 beta in the diabetic rat. | the peripheral metabolism of [3h]estradiol-17 beta (e2(3)h) was studied in ovariectomized, streptozotocin induced diabetic rats, in an attempt to understand the reduced nuclear retention time and the decreased activity on protein synthesis of e2 in diabetic rat uterus as reported recently. e2(3)h was injected intraperitoneally and the kinetics of 3h metabolites were followed in plasma extracts obtained between the time intervals of 10-210 min after injection. diabetics accumulated about 15 times ... | 1984 | 6492801 |
| perturbation of podocyte plasma membrane domains in experimental nephrosis. a lectin-binding and freeze-fracture study. | alterations in the ultrastructural organization of podocyte plasma membrane domains were quantitatively assessed in puromycin aminonucleoside-treated rats by the use of 1) helix pomatia lectin-gold complexes for detection of a specific glycocalyx component(s) normally associated with foot process bases and 2) freeze-fracture for detection of intramembrane particles and endocytotic invaginations on the plasma membrane. lectin-binding sites were significantly reduced on podocyte foot process bases ... | 1984 | 6496656 |
| lectin binding of surface epithelia and concomitant glands of gallbladder and biliary ducts in the guinea pig. | complex carbohydrate components of secretory granules and the glycocalix were analysed in surface epithelia, endoepithelial glands and exoepithelial tubuloalveolar glands of the biliary-ductular system (guinea pig). brunner glands and pyloric glands were studied for comparison. the columnar epithelial cells of the gallbladder and biliary ducts displayed a well-developed pas-positive apical glycocalix. these materials strongly bound ricinus communis a i, ulex europaeus i, lotus tetragonolobus a a ... | 1984 | 6526694 |
| simple enzymatic detection method for urinary sulfated 7 alpha-hydroxy bile acids in normal subjects and in patients with acute hepatitis. | urinary sulfated primary bile acids, 7 alpha-hydroxy bile acids, are detected by an enzymatic method using 7 alpha-hydroxysteroid dehydrogenase (ec 1.1.1.-, 7 alpha-hsd) after chromatographic fractionation on sephadex g-25. urinary sulfated or glucuronated bile acids are hydrolyzed by beta-glucuronidase/sulfatase (ec 3.2.1.31/ec 3.1.6.1) from helix pomatia and then released 7 alpha-hydroxy bile acids are detected with 7 alpha-hsd in the presence of beta-nd+, diaphorase (ec 1.6.99.2, from clostri ... | 1983 | 6574134 |
| expression of helix pomatia (hp) haemagglutinin receptors on cytolytic lymphocytes activated in mixed cultures. | the expression of receptors for helix pomatia (hp) haemagglutinin, a t cell marker, was assayed on human lymphocytes cultivated with k562 or allogeneic lymphocytes. the receptor was detected on the cells after neuraminidase treatment by reactivity with fitc conjugated hp. by affinity chromatography the lymphocyte populations were separated into 3 subsets: (1) a subset which did not attach to the lectin column; (2) and (3) two subsets attaching with different avidities, and therefore eluting with ... | 1983 | 6600771 |
| characterization of effector cells mediating igg and igm antibody-dependent cellular cytotoxicity. | spleen effector cells for igg- and igm-induced antibody-dependent cellular cytotoxicity (adcc) were characterized with respect to density and cell surface markers by using sheep erythrocytes (srbc) coated with hybridoma-derived monoclonal anti-srbc igg or anti-srbc igm antibodies as targets. while basically the same effector cells are cytolytic for igg and igm antibody-coated srbc, they differ with respect to their relative killing capacity for igg- versus igm-coated target cells. on the basis o ... | 1983 | 6601820 |
| fractionation of mouse cytotoxic t cells by use of lectins. | mouse cytotoxic t lymphocytes (ctls), induced in vivo and in vitro in mixed-lymphocyte cultures, were fractionated into agglutinated and unagglutinated cells by use of various lectins. cytolytic activity was enriched in the cell fraction agglutinated by various 2-acetamido-2-deoxy-alpha-d-galactopyranose-specific lectins, namely, dolichos biflorus agglutinin (dba), helix pomatia agglutinin (hpa), and phaseolus limensis agglutinin (lba). only a little cytolytic activity remained in the unagglutin ... | 1983 | 6605191 |
| evidence for carbohydrate-deficient forms of the major sialoglycoproteins of human platelets, granulocytes and t lymphocytes in individuals with tn syndrome. | 125i-helix pomatia and 125i-wheat germ lectins were used as probes to visualise membrane glycoproteins of circulating blood cells from individuals with tn syndrome. cells were solubilized in triton x-100 and subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis prior to incubation with iodinated lectins. normal platelets, granulocytes and t lymphocytes have a single major sialoglycoprotein of mol. wt. 143,000, 115,000 and 115,000 respectively. in tn platelets, granulocytes and ... | 1983 | 6605304 |
| flow cytometric analysis of the binding of eleven lectins to human t- and b-cells and to human t- and b-cell lines. | the relative surface binding of 11 lectins to human peripheral blood t- and b-lymphocytes, to molt-4 and jm t-cell lines, and to 6410 and nc37 b-cell lines was determined by flow cytometry. the lectins from lens culinaris (lca), ricinus communis (rca), arachis hypogaea (pna), abrus precatorius (apa), ulex europaeus (uea-f), sarothamnus scoparius (sas-f), helix pomatia (hpa), phaseolus coccineus (l-pha), glycine max (sba), and triticum vulgare (wga) were fluoresceinated and incubated with living, ... | 1984 | 6609053 |
| lectin-binding and spontaneous capping characteristics of the thymocyte glycophorin-like glycoprotein. | monoclonal antibodies against lymphocyte glycoproteins have been used to identify the membrane molecules which bind peanut (pna) and helix pomatia (hpa) agglutinins and cap spontaneously on the uropod of polarized rat and mouse thymocytes. on the basis of co-capping experiments and radiolabelling of isolated glycoproteins after sodium dodecylsulfate polyacrylamide electrophoresis (sds-page), the major hpa- and pna-binding sialoglycoprotein (with an apparent molecular weight of about 105 k; (1k = ... | 1984 | 6609831 |
| neuraminidase in bacteroides fragilis. | a neuraminidase from bacteroides fragilis was purified 542-fold by isoelectric focusing, adsorption chromatography on affi-gel 202, and gel filtration chromatography on sephadex g-200. on isoelectric focusing the neuraminidase was resolved into three differently charged fractions with pi values of 6.8, 7.1, and 7.4. the major component of pi 7.1 was used for further purification. the purified enzyme had optimal activity at ph 6.4 with n-acetylneuraminlactose as the substrate. its molecular weigh ... | 1983 | 6614909 |
| glycolipid-lectin interactions: detection by direct binding of 125i-lectins to thin layer chromatograms. | glycolipids that bind 125i-labeled lectins are detected by autoradiography after thin layer chromatography of glycolipid standards or crude lipid extracts. soybean agglutinin, bandeiraea simplicifolia i isolectins a4 and b4, and helix pomatia lectin are used to detect corresponding cell surface, glycolipid receptors in human and bovine erythrocytes. when lipid extracts from a and ab erythrocyte stroma are analyzed with helix pomatia lectin, a polymorphic expression of blood group a glycolipid de ... | 1983 | 6615536 |
| peripheral nervous system myelin and schwann cell glycoproteins: identification by lectin binding and partial purification of a peripheral nervous system myelin-specific 170,000 molecular weight glycoprotein. | radioiodinated lectins were used to detect glycoproteins of peripheral nervous system (pns) myelin (rat, human, bovine) and cultured rat schwann cells. proteins were resolved by sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis and transferred to nitrocellulose filters. the filters were overlaid with radioiodinated lectins of known saccharide affinities. these included concanavalin a, helix pomatia, limulus polyphemus, maclura pomifera, peanut, soybean, ulex europaeus, and wheat ger ... | 1983 | 6619865 |
| polymorphonuclear leukocyte-mediated cytolysis induced by animal lectin. | nine animal lectins, i.e., sarcophaga peregrina agglutinin, balanus roseus agglutinin, aplysia kurodai agglutinin, balanus balanoides agglutinin, tetraclita squamosa japonica agglutinin, misgurnus anguillicaudatus lectin, asterina pectinifera agglutinin, helix aspersa agglutinin and helix pomatia agglutinin, were tested for induction of cytolysis mediated by polymorphonuclear leukocytes. among them, s. peregrina agglutinin and b. roseus agglutinin lysed murine target cells in co-operation with p ... | 1983 | 6628907 |
| subsets of epidermal langerhans cells as defined by lectin binding profiles. | in this study we characterize the cell surface glycoconjugate moieties of strain 2 guinea pig epidermal langerhans cells (lc) in single cell suspension by using a battery of 17 fluorescent lectins. all lc displayed binding sites for concanavalin a, succinylated concanavalin a, lens culinaris agglutinin, pisum sativum agglutinin, wheat germ agglutinin, succinylated wheat germ agglutinin, griffonia simplicifolia agglutinin i, ricinus communis agglutinin i, phaseolus vulgaris e agglutinin, and phas ... | 1983 | 6631049 |
| the fate of dibenz[b,f]-1,4-oxazepine (cr) in the rat. part ii. metabolism in vitro. | cr (dibenz[b,f]-1,4-oxazepine) is metabolized by rat liver 105 000 g supernatant fractions by (a) ring opening and reduction to 2-amino-2'-hydroxymethyldiphenyl ether and (b) oxidation at c11 to give a cyclic lactam. reaction (a) is nadph-dependent, decreased by dialysis and methylene blue, whereas reaction (b) is heat-resistant, inactivated by dialysis, inhibited by cn-, p-chloromercuribenzoate, amytal and menadione, and stimulated by methylene blue, phenazine methosulphate and 2,6-dichlorophen ... | 1983 | 6636832 |
| procedure for the gas chromatographic-mass spectrometric confirmation of some exogenous growth-promoting compounds in the urine of cattle. | as gas chromatography-mass spectrometry is the most conclusive confirmation technique available today for the detection of ppb levels of anabolics in the urine of cattle, the following procedure was used. the urine is hydrolysed with helix pomatia intestinal juice, extracted, and the extract cleaned by gel-permeation chromatography or with extrelut. in one fraction are eluted diethylstilboestrol, dienoestrol, hexoestrol, methyltestosterone, ethinyloestradiol, zeranol and trenbolone. this fractio ... | 1983 | 6672034 |
| cytochemical localization of terminal n-acetyl-d-galactosamine residues in cellular compartments of intestinal goblet cells: implications for the topology of o-glycosylation. | the o-linked oligosaccharides of mucin-type glycoproteins contain n-acetyl-d-galactosamine (galnac) that is not found in n-linked glycoproteins. because helix pomatia lectin interacts with terminal galnac, we used this lectin, bound to particles of colloidal gold, to localize such sugar residues in subcellular compartments of intestinal goblet cells. when thin sections of low temperature lowicryl k4m embedded duodenum or colon were incubated with helix pomatia lectin-gold complexes, no labeling ... | 1984 | 6693488 |
| human amyloid p component: a circulating lectin that modulates immunological responses. | amyloid p component (ap/sap), a glycoprotein, precipitated with purified snail galactans from helix pomatia and arianta arbustorum in a dose-dependent manner. radiolabelled ap binds to human peripheral blood mononuclear cells (pbmc), erythrocytes, and cells derived from human non-t, non-b acute lymphocytic leukaemia. the ap cell binding is specific in that it is dose-dependent and can be blocked both by excess cold ap and by helix pomatia galactan, although it cannot be blocked by an equal amoun ... | 1984 | 6710082 |
| localization of t-lymphocyte areas in human lymphoid tissues by fluorochrome-labelled helix pomatia a haemagglutinin. | fluorochrome-labelled helix pomatia a haemagglutinin (hp) stained t-dependent areas in cryostat tissue sections of human lymph node, tonsil, and spleen and thymocytes within thymic medulla but not within thymic cortex. neuraminidase treatment of the tissue section was a prerequisite for the positive cell staining. the staining was cell-membrane-associated, and the histological pattern was essentially the same as that observed in parallel sections treated with monoclonal antibodies to t cells (ok ... | 1982 | 6753127 |
| helix pomatia agglutinin (hpa) affinity chromatography: the isolation of pure b and t cell populations and their use for the routine hla-dr (ia) serology. | the anti-a1 agglutinin (hpa) of the albumin gland of the snail helix pomatia binds specifically to neuraminidase-treated t lymphocytes. after its immobilization on sepharose-6mb an affinity matrix is obtained which is able to separate t and b lymphocytes. in 40 independent experiments enriched t and b cell populations were isolated either by this hpa affinity technique or by e-rosettes incubated with sheep red blood cells for 2 h or 15 h. the results showed that with the hpa affinity matrix with ... | 1980 | 6766169 |
| molluscan immunity: interactions between the immunogenic bacterium pseudomonas aeruginosa and the internal defense system of the snail helix pomatia. | | 1980 | 6772484 |
| [immunogenic activity of hemocyanin from the hemolymph of helix pomatia and octopus vulgaris]. | the immunogenic activity of helix pomatia haemocyanin (hph) and octopus vulgaris haemocyanin (ovh) has been tested in rabbits. primary and secondary antibody response has been evaluated in sera by agar double immuno-diffusion test (ouchterlony), counter immunoelectrophoresis and complement fixation. bath haemocyanins proved highly antigenic: antibody titers rising up to 1/3.200 after the second antigenic challenge. the ouchterlony test showed a partial identity between h.p.h. and o.v.h. no adver ... | 1981 | 6794581 |
| morphine glucuronide hydrolysis: superiority of beta-glucuronidase from patella vulgata. | beta-glucuronidase from patella vulgata, helix aspersa, helix pomatia, and bovine liver were evaluated for usefulness in routine hydrolysis of drug-glucuronic acid conjugates from equine urine samples. factors affecting the reaction rate (enzyme concentration, ligand concentration, temperature, and ph) were optimized. a 3-h incubation at 65 degrees c with 5000 u of beta-glucuronidase from p. vulgata per milliliter of urine resulted in complete hydrolysis of all morphine glucuronide in the urine ... | 1982 | 6799227 |
| lectins as markers of human epidermal cell differentiation. | the expression of sugar residues on human epidermal cells was investigated by means of lectin binding, as a way of determining membrane structural changes occurring during the differentiation of the epidermis. fourteen lectins of different sugar specificity were conjugated with fluorescein isothiocyanate (fitc-lectins) and tested in fluorescence microscopy on frozen sections of normal human epidermis. in parallel, fitc-lectins were tested on psoriatic-involved epidermis to visualize differences ... | 1982 | 6816653 |
| lectins as probes for identification of tumor-associated antigens on urothelial and colonic carcinoma cell lines. | in the search for tumor-associated antigens, seven lectins were used to investigate the cellular distribution of membrane-associated glycoproteins on a panel of human cells derived from tumor or normal tissues. surface labelled lysates of the different cells were precipitated with the lectins and the precipitates were separated on sds-page. comparison of the autoradiographic patterns revealed that a la-reactive 115k glycopeptide (gp 115) was present on transitional-cell carcinoma cells of the ur ... | 1983 | 6826254 |
| lectin-defined cell surface glycoconjugates of pancreatic cancer cells and their nonmalignant counterparts. | alterations of cell surface carbohydrates of human pancreatic cancer cells from long-term cultures (colo 357, rpmi 7451, pc 103, pc 107) were assessed ultrastructurally by use of an array of lectin-enzyme conjugates, and compared with lectin-defined changes of glycoconjugates on human pancreatic tissue sections of normal and various pathological conditions. ulex europeus and, to a lesser degree, lotus tetragonolobus lectin binding indicate that l-fucose-containing glycoconjugates are expressed p ... | 1983 | 6832449 |
| interaction of the brush-border hydrolases of the human small intestine with lectins. | the role of human intestinal brush-border membrane hydrolases in lectin binding to the apical region of the enterocyte was investigated. the crossed-immunoelectrophoresis method was used to identify six hydrolases before and after interaction with nine lectins. three lectins (phaseolus vulgaris, ulex europeus, and ricinus communis 120 agglutinins) bound the hydrolases of 5 different subjects. helix pomatia agglutinin interacted with only 1 subject. | 1983 | 6884583 |
| lectin-binding patterns of small lymphocytes in bone marrow, thymus and spleen: demonstration of lymphocyte subsets by quantitative radioautography. | cells from mouse bone marrow, thymus and spleen were exposed to 125i-labeled concanavalin a (con a). lens culinaris lectin (lcl), soybean agglutinin (sba), helix pomatia agglutinin (hpa), phytohemagglutinin-p (pha-p), peanut agglutinin (pna), or wheat germ agglutinin (wga) in a range of concentrations and examined radioautographically. small lymphocytes in the three organs differed in the minimal concentration of each lectin which gave detectable surface labeling, while at optimal lectin concent ... | 1981 | 6895730 |
| studies on the meta and para o-sulphation of the catechol compound 3,4-dihydroxybenzoic acid by rat liver sulphotransferase in vitro. | the enzymic meta and para o-sulphation of 3,4-dihydroxybenzoic acid was investigated in vitro with a dialysed high-speed supernatant from rat liver. the o-sulphated products were identified by comparison with the reference compounds. the chemical synthesis and identification of the reference o-sulphate esters is described in detail. the sulphotransferase activity of the dialysed supernatant from rat liver towards 3,4-dihydroxybenzoic acid was 580 pmol of 3-o-sulphate and 120 pmol of 4-o-sulphate ... | 1980 | 6937191 |
| cell surface characteristics of human histiocytic lymphoma cell lines. ii. expression of helix pomatia a hemagglutinin binding surface glycoproteins, hla-dr and common acute lymphocytic leukemia (call) antigen. | | 1981 | 6943398 |
| the distribution of helix pomatia lectin receptors on mouse lymphoid cells and other tissues. | the distribution of receptors for the helix pomatia lectin on mouse lymphoid cells and other tissues was investigated. using a sensitive rosetting assay combined with immunofluorescence, lectin receptors were found on the membrane of approximately 90% of peripheral t lymphocytes, 75% of thymocytes, 30% of bone marrow cells, 20% of nude spleen cells, 15-50% of peritoneal exudate macrophages, and a subpopulation of peritoneal exudate mast cells. the thy-1-positive nude spleen cells were predominan ... | 1981 | 6973473 |
| b lymphocyte specificity of lectins of cepaea nemoralis and dolichos biflorus: paradoxical binding of anti-a active lectins to human lymphocyte subclasses. | binding of four blood group a-specific lectins, from helix pomatia (hp), lucorum (hl), cepaea nemoralis (cn) and dolichos biflorus (db) to human lymphocytes was determined. use was made of lectins attached to latex particles, as convenient probes to detect binding of lectins to individual cells, specificity of lectin-latex probes was demonstrated by comparison with agglutination and fluorescent lectin binding; experiments with untreated and enzyme-treated human and animal erythrocytes confirme ... | 1981 | 6975674 |
| helix pomatia receptors on rat t lymphocytes and bone marrow cells. | helix pomatia (hp) receptors are present on approximately 60 percent of neuraminidase (nanaase)-treated rat t lymphocytes. the hp+ spleen cells (spc) can be separated from hp-, immunoglobulin-bearing (ig+) cells by affinity chromatography. the hp+ spc mediate the local graft-versus-host reaction (gvhr). less than 1% of rat lymphocytes bear both hp and ig markers. approximately 25% of lewis rat bone marrow cells are hp+ (only 1% are hp+ ig+), suggesting that the hp receptor may be a differentiati ... | 1982 | 6983492 |
| lectin receptors as markers for trypanosoma cruzi. developmental stages and a study of the interaction of wheat germ agglutinin with sialic acid residues on epimastigote cells. | trypanosoma cruzi at various stages of maturation and differentiation have been isolated by conventional cellular fractionation procedures and characterized by cell surface markers using 30 highly purified lectins encompassing all known sugar specificities. cell surface carbohydrates of the various t. cruzi stages were analyzed by agglutination and lectin-binding assays. specific receptors for wheat germ agglutinin (wga), helix pomatia, sophora japonica, and bandeiraea simplicifolia lectin ii we ... | 1980 | 7000967 |
| characterization and response to mitogens of mammary lymphocytes from the bovine dry-period secretion. | from bovine mammary secretion during the dry period, the total number of cells was between 1.2 and 5.9 x 10(6)/ml. a mean of 35% of these cells were classified as lymphocytes and approximately 85% of them could be isolated by the ficoll-isopac method. centrifugation separated 6% of the cells into the fat; 5% of them were lymphocytes. about 47% of the lymphocytes bound helix pomatia agglutinin, a t-cells marker, while the proportion of ig-bearing cells was approximately 28%. the mammary lymphocyt ... | 1980 | 7005279 |
| [isolation of yeast protoplasts using various preparations of the hepato-pancreatic juice of helix pomatia]. | conversion of large amounts of saccharomyces cerevisiae cells to protoplasts is studied using various preparations extracted from helix pomatia hepato-pancreatic juice. the most favourable yield in two hours incubations (88 per cent) is obtained with 20 ml cytohelicase, a chitinase and glucanase enriched extract, per 400 g of yeast cells, harvested at the end of the logarithmic growth phase and preincubated in presence of 2-mercaptoethanol. | 1981 | 7011422 |
| protoplasts from yeast and mycelial forms of candida albicans. | protoplasts have been obtained in high yields from the yeast and mycelial forms of a variety of strains of candida albicans by enzyme digestion of cells with commercially available lytic enzymes. the protoplast formation procedure was equally effective for exponential and stationary phase cells. pretreatment with dithiothreitol and pronase in the presence of edta and tris was necessary. other thiol reagents and conditions did not release protoplasts from all the strains of c. albicans tested. tr ... | 1980 | 7014767 |
| the occurrence of thomsen-friedenreich receptors in bovine erythrocytes of various fv genotypes. | in the v/v type of bovine erythrocytes (be), the antigen im and the thomsen-friedenreich (tf) receptors, which reacted with a tf-specific agglutinin from human sera and the lectin from helix pomatia, were all destroyed by treatment with trypsin. unlike the v/v, in the f/f type of be the antigen im and these tf receptors were not only not destroyed by trypsin, but this enzyme treatment was, in fact, necessary to make them amenable to the corresponding agglutinins. another tf receptor, which react ... | 1981 | 7024419 |
| determination of salivary abh-blood group antigens by rocket affinoelectrophoresis. | a rocket affinoelectrophoretic assay system was used to detect and quantify the human salivary abh-blood group antigens of 155 individuals. in this system blood group antigens precipitated as rockets in agarose gels containing different lectins, with the rocket height being correlated to the antigen concentration. this technique was compared with the classical hemagglutination inhibition method for determination of salivary blood group antigens, and it was demonstrated that salivary a, b and h b ... | 1982 | 7072191 |
| use of lectins for detection of glycoconjugates in the glandular cells of the human bronchial mucosa. | paraffin-embedded human bronchial biopsies, obtained in macroscopically healthy areas, were examined using nine peroxidase-bound lectins. these were either isolated or purified by affinity column chromatography (ulex europeus, triticum vulgare, glycine max, and arachis hypogatea lectins) or commercial preparations (lotus tetragonolobus, canavalia ensiformis, helix pomatia, phaseolus vulgaris, and lens culinaris lectins) and conjugated to peroxidase (except for concanavalin a). these labeled lect ... | 1982 | 7130674 |
| detection and quantitation of 19-norandrosterone in urine by isotope dilution-mass spectrometry. | a highly accurate method has been developed for detection and quantitation of 19-norandrosterone, the major urinary metabolite of 19-nortestosterone in man. a suitable 14c labelled standard was obtained by i.m. injection of [4-14c]-19-nortestosterone into a human volunteer. a fixed amount of this internal standard was added to a fixed amount of urine and the mixture was treated with helix pomatia for 24 h. after extraction and purification by t.l.c., the mixture was converted into methoxime-trim ... | 1982 | 7132356 |
| induction of macrophage-mediated tumor lysis by an animal lectin, sarcophaga peregrina agglutinin. | several animal lectins, such as sarcophaga peregrina agglutinin, limulus polyhemus agglutinin, helix pomatia agglutinin and helix aspersa agglutinin, were tested for induction of tumor lysis mediated by macrophages. among them, only s. peregrina agglutinin purified from the hemolymph of s. peregrina larvae lysed tumor cells in co-operation with macrophages from the peritoneal cavity of mice. s. peregrina agglutinin alone did not kill target tumor cells. macrophages in the presence of this lectin ... | 1982 | 7152198 |
| fructose-bisphosphate aldolase from helix pomatia. | | 1982 | 7154953 |
| molluscan immunobiology: isolation of an aeromonas formicans which escapes the internal defense system of helix pomatia. | bacterial infections are surprisingly rare in wild populations of gastropod molluscs. a strain of aeromonas formicans, which has now been isolated, kills helix pomatia, a land snail, when injected at doses greater than 10(6) viable cells g-1 snail weight. koch's postulates were fulfilled with this organism. initial clearance of aeromonas from the circulation was followed within hours by a bacteremia; since neither culture supernatants, killed cells nor cell extracts killed helix, there is eviden ... | 1982 | 7160512 |
| surface modifications in the platelets of a patient with alpha-n-acetyl-d-galactosamine residues, the tn-syndrome. | the tn-syndrome is an acquired disorder characterized by the polyagglutination of blood cells and the pathological exposure of alpha-n-acetyl-d-galactosamine residues (tn-antigen) at the cell surface. we now report studies on the platelet of a patient (ba.) of which 81% reacted positively with a fluorescein conjugate of helix pomatia agglutinin (hpa). the surface proteins of ba. platelets were labeled with 125i by the lactoperoxidase-catalyzed procedure; single and two-dimensional electrophoresi ... | 1982 | 7174794 |
| differences of glycoconjugates exposed on hypernephroma and normal kidney cells. | we analyzed the outer cell membranes of normal kidney cells, hypernephroma cells, nude mouse transplants of hypernephroma tissue, and cell lines derived from hypernephroma using horseradish peroxidase-coupled lectins of different specificity, in order to investigate their binding to cryostat sections or the cell surfaces of suspended single cells. we showed that hypernephroma cells express higher amounts of receptors for robinia pseudoacacia lectin, soy bean lectin and phaseolus vulgaris lectin ... | 1982 | 7176041 |
| [lipase activity of oospora lactis protoplasts]. | the paper describes how to prepare osmotically sensitive forms from the fungus oospora lactis. the lytic enzyme from actinomycetes at a concentration of 25 mg/ml causes abundant formation of protoplasts at 36 degrees c and ph 7.5 within 10--15 min. the best stabilizing agent is 1 m nacl solution or a mixture of 1 m nacl with 1 m mannitol solution (1:1). in order to induce lysis of cell walls by the enzyme from helix pomatia, the cells must be pretreated with a solution containing 40 mm tris (hyd ... | 1980 | 7190640 |
| simple test for detection of virus neuraminidase and antineuraminidase using lectins (lectin-neuraminidase test system). | the fast and sensitive detection of virus neuraminidase effectiveness (influenza virus a and b or mumps virus) and of antibodies against virus neuraminidase in human serum is described. lectin mainly obtained from arachis hypogaea and helix pomatia is used in the lectin-neuraminidase test (ln-test). the lectins are capable of agglutinating erythrocytes after virus incubation because virus neuraminidase reveals the t-antigen to be situated on erythrocytes. the presence of anti-neuraminidase in hu ... | 1980 | 7223125 |
| distribution of serotonin and dopamine receptors in aplysia tissues: analysis by [3h]lsd binding and adenylate cyclase stimulation. | the distribution of receptors for serotonin and dopamine has been studied in various neuronal and non-neuronal tissues from aplysia californica using: (1) a [3h]lsd binding assay; and (2) stimulation of adenylate cyclase activity. high levels of specific [3h]lsd binding were found in all ganglia and nerves examined. lower levels of binding were present in a number of muscle tissues and in the sheath surrounding the central ganglia. the ability of serotonin and dopamine to inhibit [3h]lsd binding ... | 1980 | 7357415 |
| human alkaline phosphatases. evidence of three isoenzymes (placental, intestinal and liver-bone-kidney-type) by lectin-binding affinity and immunological specificity. | the structural relationship between human alkaline phosphatase isoenzymes from placenta, intestine, liver, bone and kidney was investigated by lectin-binding affinity chromatography. in addition, antibody-binding sites of the enzymes were studied using monospecific antisera against each of the isoenzymes. evidence is offered for the existence of three classes of alkaline phosphatases: the placental isoenzyme, the intestinal isoenzyme and the liver-bone-kidney-type-isoenzyme: 1. a high affinity t ... | 1980 | 7437449 |
| differential expression of lectin receptors during hemopoietic differentiation: enrichment for granulocyte-macrophage progenitor cells. | molecular changes occur at the surface of hemopoietic cells during differentiation from progenitor cells to mature granulocytes and macrophages. the differential expression of surface carbohydrate residues has been probed using lectins and the results used to purify normal mouse granulocyte-macrophage progenitor cells. ten different lectins were screened for selective interaction with mouse hemopoietic colony-forming cells (cfcs), using agglutination or a quantitative analysis of the number of f ... | 1980 | 7440634 |
| increase of blood group a and loss of blood group sda activity in the mucus from human neoplastic colon. | aqueous extracts of human neoplastic and adjacent normal colonic mucosa were investigated for hemagglutination inhibition of both the anti-a serum and the dolichos biflorus lectin. the anti-a antiserum was inhibited by the extracts from neoplastic tissues to a much higher extent than by that from normal mucosa; the inhibition was strictly dependent on the blood group and the secretor status of the individual. the inhibition titers against the dolichos lectin were much lower for the tumor than fo ... | 1980 | 7466135 |
| lectin receptors on human blood and bone marrow cells and their use in cell separation. | a series of fluorescein-conjugated lectins (sophora japonica agglutinin, helix pomatia agglutinin, peanut agglutinin, the erythroagglutinin from phaseolus vulgaris, pokeweed mitogen, wheat germ agglutinin and the fucose-binding lectin from lotus tetragonolobus) were analyzed for their binding to human peripheral blood cells with a fluorescence-activated cell sorter. most of the lectins showed increasing cell binding in the order erythrocytes less than lymphocytes less than monocytes less than ne ... | 1980 | 7470630 |