Publications

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an improved feeding procedure for biomphalaria glabrata. 19725057242
bacteria in the "curative" effect of hemolymph of biomphalaria glabrata on schistosoma mansoni-infected mice. 19715093664
evaluation of two methods for the detection of schistosoma mansoni cercariae shed by biomphalaria glabrata. 19715105897
the arterial system of the planorbid snail biomphalaria glabrata. 19715112383
shell abnormalities in biomphalaria glabrata infected with schistosoma mansoni nd their significance in field transmission studies. 19715123699
schistosoma mansoni: respirometric and partial pressure studies in infected biomphalaria glabrata. 19715138652
glucose levels in the mollusc biomphalaria glabrata infected with schistosoma mansoni. 19715151913
schistosoma mansoni infection in biomphalaria glabrata: alterations in heart rate and thermal tolerance in the host. 19715151914
[laboratory observations on the biological control of biomphalaria glabrata by pomacea sp. (ampullaridae)]. 19715162369
biological control of biomphalaria glabrata by marisa cornuarietis in irrigation ponds in puerto rico. 19695308710
control of schistosomiasis in guayama and arroyo, puerto rico.in 1953, a programme to control schistosomiasis mansoni was started in guayama and arroyo, puerto rico, employing, first, chemical and, later, both biological and chemical methods to control biomphalaria glabrata, the snail host of schistosoma mansoni. sodium pentachlorophenate was the molluscicide, and an ampullarid snail, marisa cornuarietis, was used for biological control in ponds and reservoirs. in addition some chemotherapy was given to infected persons. the prevalence of intestinal schist ...19705309511
cost of harvesting and spreading marisa cormuarietis for biological control of biomphalaria glabrata in aibonito, puerto rico. 19705309516
the effect of sublethal doses of the molluscicide (n-tritylmorpholine) on the development of schistosoma mansoni in biomphalaria glabrata (say). 19695381131
maintenance of two feline lungworms in aquatic snails (biomphalaria glabrata). 19705437115
increased heart rate in biomphalaria glabrata parasitized by schistosoma mansoni. 19705449737
control of biomphalaria glabrata in a small reservoir by fluctuation of the water level. 19705493051
laboratory studies of the host-parasite relationship of schistosoma mansoni and biomphalaria glabrata from st lucia, west indies. 19705500111
[emergency circadian rhythm of cercariae (schistosoma mansoni-biomphalaria glabrata)]. 19715564021
labeling of biomphalaria glabrata and cercariae of schistosoma mansoni with radioselenium. 19685673655
some quantitative data on the laboratory and field infection of biomphalaria glabrata from jabuticatubas (state of minas gerais, brasil) by miracida of schistosoma mansoni. 19685679030
the reaction of australorbis glabratus (biomphalaria glabrata) to infection with schistosoma mansoni. 19685679657
intramolluscan stages of schistosoma mansoni in biomphalaria glabrata. 19695789090
antagonism of paryphostomum segregatum rediae to schistosoma mansoni sporocysts in the snail biomphalaria glabrata. 19676062083
estivation of biomphalaria glabrata (basommatophora: planorbidae). associated characteristics and relation to infection with schistosoma mansoni. 19676066227
behavioural responses to amino acids and related compounds, including propionic acid, by adult biomphalaria glabrata (say), a snail host of schistosoma mansoni. 19806104559
elimination of biomphalaria pfeifferi, bulinus tropicus and lymnaea natalensis by the ampullarid snail, marisa cornuarietis, in a man-made dam in northern tanzania.marisa cornuarietis is a well known ampullarid competitor/predator of biomphalaria glabrata in puerto rico. for the first time in africa a flourishing population of marisa has been established in a small, permanent, man-made dam at kisangara, near moshi, tanzania. prior to the release of m. cornuarietis in june 1977, this dam supported thriving populations of the pulmonate snail hosts biomphalaria pfeifferi and lymnaea natalensis; bulinus tropicus and the melaniid melanoides tuberculata were als ...19826122367
interactions between st. lucian biomphalaria glabrata and helisoma duryi, a possible competitor snail, in a semi-natural habitat.in artificial drains similar to those used in banana culture on st. lucia, helisoma duryi, the rams-horn snail, controlled biomphalaria glabrata, intermediate host of schistosomiasis on that island. time required for elimination of b. glabrata depended on environmental temperature and numbers of h. duryi initially introduced in the drains. best fit to the data was given by the equation for the logistic curve rather than by an equation for unlimited growth. multiple regression analyses of natalit ...19816123247
an isoenzyme marker possibly associated with the susceptibility of biomphalaria glabrata populations to schistosoma mansoni.nine laboratory populations and one field population of the snail host biomphalaria glabrata were compared with respect to their electrophoretic patterns for acid phosphatase (acp) and with their susceptibility to schistosoma mansoni infection. a strong correlation (r = 0.98) was noted between the frequency of the isoenzymes acp2-s and acp2-f observed in the populations and the level of snail susceptibility as determined by bioassay. the isoenzyme acp2-s was associated with susceptibility, acp2- ...19816123248
[study of the mechanisms of rejection in incompatible mollusk-schistosome pairs from infestations by means of a natural route or by microsurgical transplantations of parasitic stages].the non-compatible host-parasite combination used in these studies is an albino brazilian strain of biomphalaria glabrata (bre) and a guadeloupean strain of schistosoma mansoni (gua). this work allows us to emphasize three main points: --the failure of infestation by miracidia transplantations in the non-compatible combination indicates that the origin of resistance does not lie at the level of the tegument, but further away, in the hemolymph of the host. --the results of the histological studie ...19826131592
mimicry of snail host antigens by miracidia and primary sporocysts of schistosoma mansoni.polyvalent antisera generated in rabbits to soluble haemolymph components from schistosoma mansoni-susceptible (pr albino 'm line') and s. mansoni-resistant (10-r2) stocks of the snail biomphalaria glabrata were employed as membrane probes to determine if antigens related to snail haemolymph were produced by the early larval stages of s. mansoni (pr-1 strain). using immunofluorescence and immunoelectron microscopical methods we have demonstrated that antibodies to susceptible (anti-suscept) and ...19836191268
schistosoma mansoni and biomphalaria glabrata share epitopes: antibodies to sporocysts bind host snail hemocytes. 19836195272
immune-recognition of schistosoma mansoni primary sporocysts may require specific receptors on biomphalaria glabrata hemocytes.cellular interactions, leading to cell-mediated cytotoxicity when biomphalaria glabrata hemocytes encapsulate schistosoma mansoni sporocysts, have been investigated. rabbit antibodies (igg), when bound to antigens on sporocyst surfaces, prevent the normal cytoadherence (ca) of hemocytes from both susceptible and resistant host snails. since interference with ca occurs with even fixed sporocysts, the effect is not due to igg stimulated modulation of the parasite surface. using two antisera with s ...19846240624
[liberation of schistosoma mansoni, sambon, 1907 cercariae by biomphalaria glabrata (say, 1818) maintained in a medium with magnesium thermophosphate]. 19806294756
schistosoma mansoni: immunofluorescent detection of its antigen reacting with biomphalaria glabrata amoebocytes.the reaction of amoebocytes in the hemolymph of the infected intermediate host, biomphalaria glabrata, to schistosoma mansoni antigens has been investigated using the indirect immunofluorescence antibody test. monolayers of amoebocytes, prepared from hemolymph of infected and normal snails, were first fixed and then reacted with antisera obtained from mice infected for 7 to 9 weeks. nonspecific and cross-reactions between the antisera and monolayers of amoebocytes were eliminated by absorbing th ...19836337058
isolation and characterization of a lectin from the snail biomphalaria glabrata and a study of its combining site.the hemagglutinins from the spawn of the water snail biomphalaria glabrata were isolated by affinity chromatography on hog gastric mucin coupled to sepharose 4b. the n-acetyl-d-glucosamine eluate (0.1 m) was fractionated further on bio-gel p-300, yielding two fractions. fraction 1 had an mr of 350 000 and displayed one band in immunoelectrophoresis, but was heterogeneous in discontinuous electrophoresis. it agglutinated human red blood cells with a1 and b specificity at concentrations of 12 and ...19836360215
spirulina as a nutrient source in experimental media for maintaining the schistosome vector, biomphalaria glabrata. 19846441531
experimental studies of age-prevalence curves for schistosoma mansoni infections in populations of biomphalaria glabrata.we report the results of experimental studies of the generation of age-prevalence curves for schistosoma mansoni infections in laboratory populations of biomphalaria glabrata. within snail populations of varying sizes and age structures, the net force of infection is shown to be linearly dependent on the rate at which miracidia are introduced into the aquatic habitat of the host. for individual snails, the per capita force of infection is shown to be related to snail age and size, and the death ...19846472887
role of cellular and humoral components in the encapsulation response of biomphalaria glabrata to schistosoma mansoni sporocysts in vitro. 19846483876
isolation, characterization and functional assessment of a hemagglutinin from the plasma of biomphalaria glabrata, intermediate host of schistosoma mansoni.an agglutinin from the plasma of biomphalaria glabrata, vector of schistosoma mansoni, has been isolated and partially characterized. its isolation is a simple two-step process, yielding an essentially pure preparation that can then be manipulated free from other plasma components. it is a large glycoprotein (10(6) daltons), found in four of the five snail strains tested, and possesses affinity for galactose-type sugar residues. based upon criteria such as molecular weight, carbohydrate and eryt ...19846500135
chronobiological aspects of the host-parasite relationships between biomphalaria glabrata and schistosoma mansoni: cercarial production and infectivity, and growth kinetics of the host. 19846501917
[biomphalaria glabrata in the state of piaui].the occurrence of biomphalaria glabrata is recorded for the first time in the state of piaui, where it was collected from several breeding places in the city of parnaíba. examination of 694 specimens showed that a part of them were infected with trematodes other than schistosomatidae. so far no autochthonous cases of schistosomiasis have been identified in the city. the presence of b. glabrata in parnaíba extends by 20 km eastward its range on the northern coastal region of the great northeaster ...19846535921
[new foci of transmission of schistosoma mansoni in the state of pará].two new foci of transmission of schistosoma mansoni in the state of pará are recorded, with the finding of naturally infected biomphalaria glabrata in the municipalities of viseu and belém. uninfected specimens of biomphalaria straminea, as well as the planorbid species biomphalaria schrammi, drepanotrema lucidum and d. anatinum, were found in the same area.19846535922
[chemoprophylaxis of schistosomiasis: molluscacidal activity of natural products--assays with adult snails and oviposition].data concerning the molluscicidal activity of 159 extracts from 84 brazilian plants on biomphalaria glabrata, the most important intermediate host of schistosoma mansoni in brazil, are presented. seventy eight extracts (49,0%) showed activity against snails or eggs, but only twenty nine (18.2%) were active on both, snails and eggs. extracts of two species (mikania hirsutissima and qualea multiflora) have shown to be lethals to adult snails at 10 ppm concentration.19846548884
[experimental infection of biomphalaria glabrata with schistosoma mansoni by the injection of miracidia]. 19836672864
schistosoma mansoni: long-term maintenance of clones by microsurgical transplantation of sporocysts.schistosoma mansoni sporocysts originally derived from monomiracidially infected biomphalaria glabrata snails were serially transplanted into the cephalopedal sinus of anesthetized snails by the microsurgical implantation of fragments of parasitized hepatopancreas and ovotestis. three to six passages each of five male and five female clones were maintained for as long as 2.0 years. of the recipient snails which survived surgery, 87% released cercariae, usually beginning 5-7 weeks after surgery. ...19846692880
[experimental schistosomiasis iii. observations on the infestation of biomphalaria glabrata by a miracidium of schistosoma mansoni].380 b. glabrata infected with 1 miracidium of s. mansoni produced 87 positive snails (22,9%), 38 of which sending out male cercariae and 49 female cercariae. the snails belonged to 3 experimental series kept at 27 degrees and 24 degrees c for 15 weeks under continuous lighting. there was no difference between the proportions of both sexes. the survival of the positive snails was favoured by the male sex. the shedding of female cercariae was greater than shedding of male. during those 15 weeks, t ...19846721371
effects of temperature on the larval development of angiostrongylus cantonensis in the intermediate host, biomphalaria glabrata.the effects of temperature on the larval development of angiostrongylus cantonensis in biomphalaria glabrata were studied under controlled conditions. when b. glabrata were maintained at different, constant temperatures, the first-stage larvae developed to third-stage larvae between 20 degrees and 31 degrees c. the velocity of development to the third-stage in the snail depended on the relationship, y = -0.1281 + 0.0081 x (y velocity of development; x rearing temperature). the "threshold of deve ...19846741223
infectivity of a microsporidium of mosquitoes (nosema algerae) to larval stages of schistosoma mansoni in biomphalaria glabrata. 19806778833
schistosoma mansoni: quantification of skin penetration and early migration by differential external radioassay and autoradiography.eleven gamma-emitting radionuclides (49sc, 54mn, 59fe (+2 and +3), 60co, 65zn, 75se (as selenomethionine, selenocystine, selenite and selenate), 109cd, 125sb, 133ba, 137cs and 203hg) were screened as labelling agents for schistosoma mansoni cercariae by incubation of infected biomphalaria glabrata snails in radioactive solution according to the technique of christensen (1977). only [75se]methionine yielded satisfactorily labelled cercariae. multiple regression analysis of volume, number of cerca ...19826803218
acquired resistance in snails. induction of resistance to schistosoma mansoni in biomphalaria glabrata. 19836874229
introduction of biomphalaria glabrata to egypt and other african countries. 19826926781
concanavalin a-induced receptor redistribution on biomphalaria glabrata hemocytes: characterization of capping and patching responses. 19817024423
further characterization of acquired resistance in biomphalaria glabrata.the albino strain of biomphalaria glabrata is capable of developing an acquired resistance to echinostomes that is relatively specific, i.e., strong against echinostoma lindoense, challenged by the homologous species, moderate or weak against closely related e. paraensei and e. liei, and nonexistent against paryphostomum segregatum and schistosoma mansoni. the present results show that acquired resistance induced by e. paraensei is much stronger against the heterologous e. lindoense than against ...19827119983
swellengrebel lecture: survival of schistosoma mansoni and other trematode larvae in the snail biomphalaria glabrata. a discussion of the interference theory. 19827123644
[evaluation of 10 years of research on s. mansoni schistosomiasis a in the french west indies].from 1971 until 1982, we studied the ecological factors which stead fast a perennial schistosomiasis in guadeloupe on an exhaustive mode. more recently, we start a similar investigation in martinique. in guadeloupe the single vector of schistosoma mansoni is the snail biomphalaria glabrata which is an ubiquist species. but the transmission to man is restricted to the "canal-sewers" where faeces are dropped and not in the rivers of basse-terre or the ponds of grande-terre. moreover, the infection ...19827126776
population structure in biomphalaria glabrata: examination of an hypothesis for the patchy distribution of susceptibility to schistosomes.it has been suggested that the patchy distribution of snail susceptibility to schistosomes might be due to genetic drift. since direct estimation of genes for susceptibility is not feasible in population studies, electrophoretic techniques were used to examine this hypothesis for populations of biomphalaria glabrata from puerto rico. these populations were characterized by relative genetic homogeneity within populations and differentiation among local populations. the data presented are consiste ...19827149103
laboratory comparison of the relative success of biomphalaria glabrata stocks which are susceptible and insusceptible to infection with schistosoma mansoni.a method of interrupting the life-cycle of the human blood fluke schistosoma by increasing the proportion of genetically insusceptible intermediate host snails in natural populations was first proposed nearly 25 years ago. the method assumes that insusceptible snails will be at a selective advantage over susceptible snails when the schistosome parasite is present, and therefore natural selection will act to increase the proportion of alleles for insusceptibility. a major objection to the propose ...19836856336
use of praziquantel in patients with schistosomiasis mansoni previously treated with oxamniquine and/or hycanthone: resistance of schistosoma mansoni to schistosomicidal agents.fourteen patients with active schistosomiasis mansoni in spite of previous treatment with oxamniquine and/or hycanthone were treated with praziquantel, single oral dose of 45 to 50 mg/kg body-weight. all underwent clinical, laboratory and electrocardiographic examination before and after treatment. untoward effects (dizziness, drowziness, nausea and abdominal pain) were observed in ten. laboratory findings disclosed no significant alteration and the electrocardiograms showed no abnormalities. mo ...19827179419
[comparative study of the susceptibility of biomphalaria glabrata populations of belo horizonte (mg), to schistosoma mansoni infection and to molluscacides]. 19827182912
lectin-binding specificities of hemocytes from two strains of biomphalaria glabrata as determined by microhemadsorption assays. 19807192226
effect of schistosoma mansoni on glycogen synthase and phosphorylase from biomphalaria glabrata (mollusca). 19826804614
schistosoma mansoni: agglutination of sporocysts, and formation of gels on miracidia transforming in plasma of biomphalaria glabrata.the resistance or susceptibility of biomphalaria glabrata strains to strains of schistosoma mansoni, the human blood fluke, are evidenced by the responses of snail hemocytes to sporocysts of the schistosome, both in vivo and in vitro. it is now reported that living sporocysts of the pr1 strain of s. mansoni agglutinate in the plasma of all tested strains of b. glabrata, in contrast to fixed sporocysts which agglutinate only in plasma from resistant snail strains. the agglutinating activity in re ...19846745386
[life cycle of clinostomum golvani n. sp. (trematoda : clinostomidae) a larval parasite of biomphalaria glabrata, the snail vector of schistosoma mansoni in guadeloupe (author's transl)].the life cycle of a new species of clinostomidae, clinostomum golvani, described in guadeloupe (french west indies). the first intermediate host is the snail vector of schistosoma mansoni in this island, biomphalaria glabrata, which can be sterilized by this parasite. poecilia reticulata (guppy) serves as the second intermediate host. adult worms were obtained under experimental conditions from butorides virescens (the definitive host in nature), nycticorax nycticorax and ardea purpurea. the adu ...19807224533
[specificity and active principle of bacillus thuringiensis var. israelensis].bio-assays of b. thuringiensis var. israelensis on larvae of drosophila melanogaster, musca domestica, culicoides sp. and chironomus plumosus are negative except in the last case, but only with doses much higher than those which are lethal for mosquito larvae. tests on mollusks (biomphalaria glabrata) and golden fishes are also negative. the specificity of action of the h 14 serotype, limited to a few diptera families, should be mainly related to the special characteristics of its crystals, whic ...19817249246
lysosomal enzyme activities in susceptible and refractory strains of biomphalaria glabrata during the course of infection with schistosoma mansoni.the distribution and abundance of the lysosomal enzyme markers, acid phosphatase (ap), peroxidase (po), and nonspecific esterase (ne), within circulating blood cells (hemocytes) were examined in a schistosome-susceptible (pr albino m-line) and a resistant (10-r2) strain of biomphalaria glabrata during the course of infection with schistosoma mansoni. the dynamics of serum (cell-free hemolymph) ap activities and total hemocyte numbers in infected snails also were investigated. hemocyte subpopulat ...19836674450
purine metabolism in biomphalaria glabrata under starvation and infection with schistosoma mansoni.purine content and its alterations during a starvation period of 29 days and an infection period of 52 days were studied. during starvation the amount of uric acid increased from 2.11 +/- 0.95 mg/g dry wt in control snails to a maximum of 6.73 +/- 3.99 mg/g dry wt on day 29 of starvation for uric acid and from 4.73 +/- 2.10 mg/g dry wt in control snails to a maximum of 11.29 +/- 3.48 mg/g dry wt on day 23 of starvation for guanine. during infection there was an increase with both uric acid and g ...19836641159
effects of alterations in the heartbeat rate and locomotor activity of schistosoma mansoni-infected biomphalaria glabrata on cercarial emergence.during a 27-hr period of food deprivation, biomphalaria glabrata infected with schistosoma mansoni had lower heartbeat rates and locomotor activities than did controls that were feeding ad lib. however, there was no difference between the cercarial emergence from control and experimental snails either before, during, or after the period of food deprivation. no correlation was found between the locomotor activity of the snail and the number of cercariae emerging from the snail in continuous light ...19836631637
altered feeding response as a cause for the altered heartbeat rate and locomotor activity of schistosoma mansoni-infected biomphalaria glabrata.biomphalaria glabrata infected with schistosoma mansoni for 33 days fed more often than uninfected snails. whereas uninfected snails had nocturnal increases in feeding, snails with a 33-day-old infection of s. mansoni fed as often during the day as in the night. using direct observation and film analysis, we found that feeding increased the heartbeat rate and locomotor activity of b. glabrata. when snails were allowed to feed ad lib., infected snails had higher heartbeat rates than uninfected sn ...19836631636
the adenylate nucleotide pool in the digestive gland-gonad complex of biomphalaria glabrata infected by schistosoma mansoni.the levels of adenylate nucleotides were examined in the digestive gland and ovotestes of biomphalaria glabrata during cercarial shedding of schistosoma mansoni, 10 weeks post-infection. in general, parasitization resulted in decreases in the adenylate levels in both tissues, but the results were not statistically significant. moreover, the energy charge ratio was not significantly altered. the mean energy charge in ovotestes from uninfected and infected individuals was 0.81 and 0.77, respective ...19846527694
selective interference with granulocyte function induced by echinostoma paraensei (trematoda) larvae in biomphalaria glabrata (mollusca).various trematode larvae can interfere with the host snail's resistance to the same or unrelated trematode species, chiefly, it appears by interference with the function of the host's granulocytes. in biomphalaria glabrata infected with the trematodes, echinostoma paraensei, granulocytes lose their ability to encapsulate the larvae of trematodes to which the hosts were previously resistant. however, the granulocytes in these snails retain their ability to encapsulate injected latex spheres, or l ...19817328451
[activities of a schistosoma mansoni focus of transmission in martinique (french antilles)].a focus of intestinal schistosomiasis was discovered in november 1981 in martinique in the saint-pierre area. an analysis of its structure and of the mecanisms of the transmission of the parasite was carried out between 1981 and 1983. this focus presents the following particularities: a transmission site very small constituted by water cress beds; a small human population infected with a prevalence of 13% (positive stools) to 41,3% (positive serologies); a weak fecal contamination of the water; ...19846524823
[evolution of spopocysts of schistosoma mansoni sambon 1907 in biomphalaria glabrata (say 1818) and biomphalaria tenagophila (d'orbigny 1835)]. 19817336119
[susceptibility to schistosoma mansoni infection of biomphalaria glabrata and biomphalaria tenagophila in the federal district, brazil]. 19817345560
is planorbarius metidjensis compatible with schistosoma haematobium and s. bovis?on the basis of laboratory snail infection experiments it is concluded that planorbarius metidjensis is unimportant in the epidemiology of schistosoma haematobium in north west africa. 410 p. metidjensis spain were exposed to 8 isolates of s. haematobium from ghana, kenya, madagascar, mauritius, morocco, tanzania, south africa and sudan. 50 p. metidjensis morocco and 50 p. metidjensis algarve were exposed to s. haematobium morocco. all infections failed to develop to maturity. serial sections of ...19846509700
the destruction of schistosoma mansoni mother sporocysts in vitro by amoebae isolated from biomphalaria glabrata: an ultrastructural study. 19807365267
differential leukocytic responses of biomphalaria glabrata to infection with schistosoma mansoni. 19807365270
intergeneric reproductive stimulation and parthenogenesis in schistosoma mansoni.when mice were infected with female cercariae of schistosoma mansoni and male cercariae of schistosomatium douthitti, many mixed pairs formed. the paired females were approximately the same size as those in unisexual infections, far smaller than females paired with s. mansoni males. although the sch. douthitti males possessed well-developed testes, sperm were not found in their female partners, which developed scanty vitelline glands and produced laterally spined eggs typical of s. mansoni. such ...19846504564
experimental pathogenicity of vibrio parahaemolyticus for the schistosome-bearing snail biomphalaria glabrata.the bacterium vibrio parahaemolyticus was found to be pathogenic for the schistosome intermediate host biomphalaria glabrata (say). when administered topically, a nonenteritis-associated strain of the bacterium had an ld50 (median lethal dose) of 6.8 x 10(7) cells per snail. a 5% trichloroacetic acid (tca) extract from v. parahaemolyticus was found to kill b. glabrata. sublethal effects of v. parahaemolyticus include shell deterioration and increased heart rate. both albino aquarium populations ...19807378944
macrophagelike hemocytes of resistant biomphalaria glabrata are cytotoxic for sporocysts of schistosoma mansoni in vitro.when schistosoma mansoni miracidia penetrate resistant individuals of the intermediate host snail biomphalaria glabrata, the sporocyst is encapsulated by hemocytes (macrophagelike cells of the snail circulation) and killed. in our in vitro model the same fate requires only sporocysts and snail hemolymph. however, when cultured in plasma alone (cell-free hemolymph), sporocysts remain viable for more than 3 days, regardless of whether the plasma is from susceptible or resistant snails. when hemoly ...19807391885
inducement of miracidia-immobilizing substance in the hemolymph of biomphalaria glabrata. 19807409973
schistosoma mansoni: cytotoxicity of hemocytes from susceptible snail hosts for sporocysts in plasma from resistant biomphalaria glabrata. 19807428914
[biological competition between biomphalaria glabrata (say, 1818) and biomphalaria tenagophila (d'orbigny, 1835), in natural breeding areas in the municipality of ourinhos, s.p. (brazil)]. 19807433874
tissue reactions induced by schistosoma mansoni in biomphalaria glabrata.in biomphalaria glabrata with a strong natural resistance, schistosoma mansoni sporocysts are rapidly encapsulated by granulocytes and killed, mainly by the strong phagocytic activity of the cells. irradiated echinostoma paraensei sporocysts seem able to suppress the function of the granulocytes. tissue reactions in snails with self-cure demonstrate: involvement of two types of cells, granulocytes and hyalinocyte-like cells; formation of amoeba-fibrous capsules; limited tendency of granulocytes ...19807436601
abnormal development of the digestive gland in biomphalaria glabrata. 19807440998
schistosoma mansoni: passive transfer of resistance by serum in the vector snail, biomphalaria glabrata.passive transfer of natural resistance to schistosoma mansoni (pr-1 strain) has been successfully accomplished in the snail intermediate host, biomphalaria glabrata (pr albino, m-line strain). injection of serum (cell-free hemolymph) from a naturally schistosome-resistant strain of b. glabrata (10-r2) into pr albino snails induced a complete protection from a primary infection with the parasite in 29 of 48 snails (60.4%). in comparison, inoculation of homologous pr albino serum or heterologous p ...19846479289
the boqueron project after 5 years: a prospective community-based study of infection with schistosoma mansoni in puerto rico.the boqueron schistosomiasis project is a prospective community-based study of schistosoma mansoni infection after the interruption of transmission by nonchemotherapeutic control measures. the study methods and the parasitologic results of the first five annual stool surveys are described in this report. in the first year, 1972, among 904 inhabitants (88% of the total population) the prevalence of infection was 40%, and the geometric mean intensity of infection among positives was 16.1 eggs per ...19807446813
metabolic interrelationship of parasitic trematodes and molluscs, especially schistosoma mansoni in biomphalaria glabrata. 19807456639
[cellular immunity in mollusk hosts of schistosomes. primary results regarding biomphalaria glabrata].cell culture are obtained from the saccular part of the kidney of biomphalaria glabrata in a medium derived from schneider drosophilia medium. two types of cells migrate from the explant: numerous amoebocytes and some fibroblasts. migration of amoebocytes appears to be regular. these amoebocytes are able to encapsulate and destroy particules and dead miracidiums. amoebocytes from incompatible snails rapidly surround miracidiums and early larvae (primary sporocysts) whereas compatible miracidium ...19807460123
emergence of third-stage larvae of angiostrongylus costaricensis morera and cespedes 1971 from biomphalaria glabrata (say). 19807463259
production of schistosoma mansoni daughter sporocysts from mother sporocysts maintained in synxenic culture with biomphalaria glabrata embryonic (bge) cells.in vitro production of schistosoma mansoni daughter sporocysts (ds) from miracidium-derived mother sporocysts (ms) was achieved by synxenic larval cultivation with cells of the biomphalaria glabrata embryonic (bge) cell line. the in vitro growth and viability of ms cocultured with bge cells or in bge cell-conditioned medium were significantly extended beyond that of larvae cultured in fresh medium alone. however, complete ds development and emergence from ms were achieved only in the presence of ...19957472861
an ultrastructural study of the effect of parasitism by larval schistosoma mansoni on the calcium reserves of the host, biomphalaria glabrata.the tissues of biomphalaria glabrata contain three types of calcium cells which can be differentiated by their location and the size and number of their contained corpuscles. x-ray analysis has confirmed the presence of calcium and magnesium in the corpuscles. molluscs containing the larval stages of schistosoma mansoni at 40 days post infection show disintegration of the calcareous corpuscles in type-a calcium cells and erosion of the inner surface of the shell.19846467339
[selection of genotypes of schistosoma mansoni and their maintenance by sporocyst transplantation].schistosoma mansoni was isolated by hatching eggs obtained from a naturally infected rat in grand etang, guadeloupe; fifty biomphalaria glabrata were exposed to five miracidia each. the resulting cercariae were used to infect laboratory mice which were later sacrificed to provide worms for enzyme analyses and eggs for further infections. seven enzymes in extracts of individual worms were examined by isoelectric focusing in polyacrylamide gels: acp, g6pdh, pgm, gpi and hk showed no variation, whe ...19846437612
invertebrate host-parasite relationships: convergent evolution of a tropomyosin epitope between schistosoma sp., fasciola hepatica, and certain pulmonate snails.monoclonal antibodies (mab) directed against schistosoma mansoni tropomyosin isoform, smtm (xu et al. experimental parasitology 69, 373-392, 1989), were used to test for cross-reactivity with biomphalaria glabrata antigens. one mab (1f10) recognized antigens of 39, 41, and 80 kda in a snail head/foot antigen preparation but not a hepatopancreas antigen preparation. another mab (1c1) cross-reacted with a 39-kda antigen in the head/foot extract but not in the hepatopancreas extract. epitope mappin ...19947512930
[susceptibility of biomphalaria tenagophila and biomphalaria glabrata from a same region to 2 schistosoma mansoni strains].b. tenagophila snails from ouro branco, mg, showed positivity for s. mansoni, with infection rates of 5%, 10%, (sj strain), and 1% (le strain) using a pool of miracidia. the mollusks were found to be susceptive from the 3rd generation reared in laboratory onwards. the b. tenagophila (ob, mg) when individually exposed to 10 miracidia, showed infection rate of 2% for le strain. b. glabrata snails from gagé, mg, showed a positivity rate of 58% for s. mansoni (le strain), under experimental conditio ...19947569607
production of schistosoma mansoni cercariae by biomphalaria glabrata from a focus in belo horizonte, minas gerais.the snail density, levels of infection and the monthly production of schistosoma mansoni cercariae by biomphalaria glabrata were determined in a focus of barreiro de baixo (belo horizonte, mg, brazil). during a period of 38 months (1984 to 1987) 5,366 snails were collected of which 324 (6.03%) were infected with s. mansoni. the total number of cercariae shed was 5,667,312. each snail shed an average of 17,422 cercariae during the time that it was under study in the laboratory. the greatest longe ...19947569620
effects of starvation and infection with schistosoma mansoni on the release rate of free amino acids (faa) by biomphalaria glabrata.1. the elimination of dissolved free amino acids (faa) by individual snails during a period of 2 hr was measured. the faa concentration in the hemolymph and in the homogenate also was determined. 2. for uninfected, normally fed snails a daily faa production of 0.7 +/- 0.25 mumol glycine equivalents/g live wt was found. with snails starving for 7 days the daily faa release rate fell to a mean value of 0.44 +/- 0.19 mumol glycine equivalents/g live wt. infection with schistosoma mansoni, however, ...19827172633
schistosoma mansoni: comparison of cloned tropomyosin antigens shared between adult parasites and biomphalaria glabrata.rabbit antisera, raised against whole, homogenized hepatopancreas from uninfected biomphalaria glabrata, were used to screen a cdna library prepared from adult schistosoma mansoni. of 1.8 x 10(5) recombinants screened, 34 clones were specifically immunoreactive with the antisera. twenty of the clones were subcloned for further analysis, and 13 of the 20 were demonstrated to hybridize to a cdna probe encoding schistosome tropomyosin i (psmtm) at high stringency (xu et al. experimental parasitolog ...19937685709
hemocytes of schistosome-resistant and -susceptible biomphalaria glabrata recognize different antigens on the surface of schistosoma mansoni sporocysts.a cytoadherence assay was used to determine whether antibodies to plasma and hemocyte components of schistosoma mansoni-susceptible (m-line) and -resistant (10-r2, 13-16-r1) strains of biomphalaria glabrata affected attachment of hemocytes to chemically fixed schistosome sporocysts differentially. experiments used purified, intact igg and purified fab fragments of each antibody. indirect fluorescent antibody tests confirmed that the intact purified igg to plasma and hemocytes from the 3 strains ...19957707192
comparative infectivity and survival of first-stage larvae of angiostrongylus cantonensis and angiostrongylus malaysiensis.angiostrongylus malaysiensis was isolated from wild rats rattus norvegicus in a seaport area of kitakyushu city, kyushu, japan in 1990. the infectivity and survival capacities of first-stage larvae of a. malaysiensis and angiostrongylus cantonensis were compared under various conditions. infectivity of first-stage larvae of the former in biomphalaria glabrata was much lower than that of the latter. in an exposure at various phs, and with various proteases and tissue homogenates of b. glabrata, t ...19957707198
spatial distribution patterns of the sporocyst infrapopulation of schistosoma mansoni within its mollusc host (biomphalaria glabrata): an unusual phenotype of aggregation.a study carried out on the intramolluscan development of the larval stages of schistosoma mansoni from venezuela in its intermediate host biomphalaria glabrata shows the existence of 2 patterns for the spatial distribution of daughter sporocysts within the digestive gland of the snail. besides the normal pattern, which is a random distribution of daughter sporocysts within the gland, 11% of the infected snails harbored an infection phenotype with daughter sporocysts strongly aggregated in only t ...19957707215
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