| opisthorchis viverrini and opisthorchiasis: a historical review and future perspective. | opisthorchiasis is caused by the liver fluke, opisthorchis viverrini. the fluke afflicts approximately seven million inhabitants in northeastern and northern thailand. the fluke utilizes, respectively, freshwater snails and cyprinoid fish as its first and second intermediate hosts. man is the accidental definitive host who acquires infection through the consumption of improperly cooked fish. information pertaining to the history, the life-cycle, the epidemiology and transmission dynamics, and di ... | 2003 | 14611871 |
| taxonomy and biology of liver flukes. | opisthorchis viverrini is a human liver fluke endemic in thailand, lao pdr and cambodia. three subspecies of bithynia siamensis and 18 species of cyprinoid fish are susceptible first and second intermediate hosts, respectively. the differentiation between the adult worm of o. viverrini and that of the closely related species, opisthorchis felineus and clonorchis sinensis, is mainly based on the shape and position of the testes and the arrangement of the vitellaria. eggs of these flukes are morph ... | 2003 | 14611872 |
| epidemiology of food-borne trematodes and other parasite infections in a fishing community on the nam ngum reservoir, lao pdr. | food-borne trematodes (fbt) are important causes of parasitic infections in many asian countries. parasitological surveys in xai udom, a small fishing community on the nam ngum reservoir, lao people's democratic republic, revealed an overall parasitic infection rate in may 1999 of 68.8% (n = 173) and in december 1999 of 65.9% (n = 261). the liver fluke, opisthorchis viverrini accounted for most of the infections (prevalences of 53.8% and 42.1%, during the first and second surveys, respectively). ... | 2006 | 17333758 |
| early stage biliary and intrahepatic migration of opisthorchis viverrini in the golden hamster. | in thailand, infection with the liver fluke opisthorchis viverrini is a serious health problem, with over 8.6 million human infections each year. early stage biliary intrahepatic migration and infection of o. viverrini in the syrian golden hamster were used to study the growth and development of this fluke. fifty metacercariae of o. viverrini were introduced into each hamster by gastric intubation. worms were found to migrate rapidly from the stomach to the gall bladder and hepatic duct, where t ... | 2007 | 17381865 |
| epidemiology of opisthorchis viverrini. | opisthorchis viverrini is a food borne trematode, important because of the sheer numbers of people infected and its serious morbidities such as hepatobiliary diseases and cholangiocarcinoma (chca). although infections are identified throughout southeast asia, the epi-center is northeast thailand, where high prevalence coexists with a high incidence of chca. in this review, we present the basic population features and the factors influencing transmission between the different hosts. | 2003 | 14611873 |
| immunology and molecular biology of opisthorchis viverrini infection. | opisthorchiasis is the major public health problems in laos pdr and thailand. the disease becomes chronic and persists for many years, leading to hepatobiliary disease and cholangiocarcinoma. less severe manifestations include cholangitis, chronic cholecystitis and cholelithiasis. a significant degree of humoral and cell mediated immune responses to the parasite can be detected both in patients and animal models. the patients igg levels appear to correlate with gall bladder size and dysfunction ... | 2003 | 14611874 |
| parasitic diseases of the biliary tract. | objective: parasites residing in the biliary tree include clonorchis sinensis, opisthorchis viverrini, opisthorchis felineus, and fasciola hepatica. they are willowy, leaf-like, flat flukes dwelling in the bile ducts and gallbladder. human ascarides, ascaris lumbricoides, dwelling in the small intestine, inadvertently migrate into the bile ducts and cause biliary obstruction. the purpose of this article is to illustrate typical imaging findings of liver fluke infection and biliary ascariasis. co ... | 2007 | 17515382 |
| the potential usefulness of the modified kato thick smear technique in the detection of intestinal sarcocystosis during field surveys. | a total of 479 stool specimens were collected from rural communities of ubon ratchathani province, thailand and examined by two techniques: the modified kato thick smear and the direct smear. the prevalence of opisthorchis viverrini (14.8%), hookworm (10.2%), sarcocystis spp (4.6%), taenia spp (2.9%), strongyloides stercoralis (2.1%), giardia lamblia (1.2%), echinostoma spp (0.6%), ascaris lumbricoides (0.4%), entamoeba histolytica (0.2%), chilomastix mesnili (0.2%) and endolimax nana (0.2%) wer ... | 2007 | 17539271 |
| epidemiology of liver cancer: an overview. | the incidence of liver cancer varies widely throughout the world, with high rates in sub - saharan africa, eastern and southeastern asia, and melanesia and a low incidence in northern and western europe and the americas. primary cancers of the liver in adults are of two main histological types: hepatocellular carcinoma, which is derived from hepatocytes, and cholangiocarcinoma, which is derived from the epithelial lining of the intrahepatic bile ducts. hepatocellular cancer is a frequently occur ... | 2004 | 15244512 |
| increased cell proliferation of mouse fibroblast nih-3t3 in vitro induced by excretory/secretory product(s) from opisthorchis viverrini. | infection by opisthorchis viverrini is a strong risk factor for cholangiocarcinoma. however, the mechanism by which the parasite is involved in carcinogenesis is not clear. in addition to the direct damage of the bile duct epithelium via direct contact with o. viverrini, the excretory/secretory (es) product(s) released from the parasites may play important roles in this process. we therefore investigated the responses of a fibroblast cell line, nih-3t3, to es product(s) released from o. viverrin ... | 2004 | 15521634 |
| discrimination of opisthorchis viverrini from haplorchis taichui using coi sequence marker. | this study aimed to discriminate infections of two common fish-borne trematodes in thailand, opisthorchis viverrini from haplorchis taichui, based on mitochondrial cytochrome c oxidase subunit i (coi) gene. designed primers (coi-ov-hap f&r primers) amplified partial coi fragments of o. viverrini and h. taichui with high sensitivity in different developmental stages (adult, metacercaria, and egg). polymerase chain reaction (pcr) amplicons were generated with low genomic dna concentration ( approx ... | 2007 | 17574199 |
| gene discovery for the carcinogenic human liver fluke, opisthorchis viverrini. | cholangiocarcinoma (cca)--cancer of the bile ducts--is associated with chronic infection with the liver fluke, opisthorchis viverrini. despite being the only eukaryote that is designated as a 'class i carcinogen' by the international agency for research on cancer, little is known about its genome. | 2007 | 17587442 |
| opisthorchis viverrini antigen induces the expression of toll-like receptor 2 in macrophage raw cell line. | opisthorchis viverrini infection induces inflammation in and around the bile duct, leading to cholangiocarcinoma in humans. to examine the mechanism of o. viverrini-induced inflammatory response, we assessed the expression of toll-like receptors (tlrs) in raw 264.7 macrophage cell line treated with an extract of o. viverrini antigen. flow cytometry and immunocytochemistry showed that o. viverrini antigen induced the expression of tlr2 but not tlr4. western blotting and immunocytochemistry reveal ... | 2005 | 15862573 |
| freshwater mollusks of medical importance in kalasin province, northeast thailand. | a snail survey was performed in six districts around irrigation areas of lampao dam, in kalasin province. the survey caught a total of 5,479 live snails and classed them into five families, 12 genera and 15 species, of which 7 species are suspected of transmitting human parasitic diseases. the seven species were pila polita, pomacea canaliculata, filopaludina (s.) m. martensi, bithynia (digoniostoma) siamensis goniomphalos, melanoides tuberculata, radix rubiginosa, and indoplanorbis exustus. of ... | 2005 | 16124433 |
| high prevalence of liver and intestinal fluke infections among residents of savannakhet province in laos. | the prevalence of liver and intestinal fluke infections was surveyed on residents of savannakhet province, laos. fecal specimens were collected from a total of 981 residents in 4 mekong riverside villages and examined by the kato-katz thick smear technique. the results revealed that the overall helminth egg positive rate was 84.2%, and the positive rate for small trematode eggs, including opisthorchis viverrini, heterophyids, or lecithodendriids, was 67.1%. to obtain adult flukes, 38 small trema ... | 2007 | 17876167 |
| cytokine expression in hamsters experimentally infected with opisthorchis viverrini. | the cytokine mrna expression of il-12, ifn-gamma, tgf-beta, il-4, and il-10 were investigated in spleen, liver and mesenteric lymph nodes (mln) in hamsters experimentally infected with opisthorchis viverrini. animals were infected with 5, 25 or 100 metacercariae (mc) and examined by rt-pcr and real-time pcr at 2 weeks, 2 and 6 months after infection. the cytokine expression was compared using hprt. the il-12 was significantly expressed at 2 weeks in the liver of the 5- and 25-mc-infected groups. ... | 2007 | 17266743 |
| biliary parasitic diseases including clonorchiasis, opisthorchiasis and fascioliasis. | parasitic infection of the biliary tree is caused by liver flukes, namely clonorchis sinensis and opisthorchis viverrini. these flukes reside in the peripheral small bile ducts of the liver and produce chronic inflammation of the bile duct, bile duct dilatation, mechanical obstruction, and bile duct wall thickening. on imaging, peripheral small intrahepatic bile ducts are dilated, but the large bile ducts and extrahepatic bile ducts are not dilated or slightly dilated. there is no visible caused ... | 2008 | 17934771 |
| artemisinins and synthetic trioxolanes in the treatment of helminth infections. | helminthiases, including schistosomiasis and food-borne trematodiasis, affect millions of people. just a few drugs are used for the treatment and control of these diseases. we review recent in-vitro and in-vivo observations with the artemisinins and synthetic trioxolanes against major trematode infections, update clinical findings, and discuss the potential impact that artemisinin-based combination therapy might have on trematode infections in settings where malaria and helminthiases are co-ende ... | 2007 | 17975411 |
| changing patterns in intestinal parasitic infections among southeast asian laborers in taiwan. | in order to determine whether there are changing patterns in intestinal parasitic infections among foreign laborers in north taiwan, one fecal specimen was obtained from each of 1,569 laborers and examined by the formalin-ethyl acetate sedimentation technique. the prevalence of intestinal parasitic infections was 8.2%. females (11.7%) had a significantly higher prevalence than males (6.9%), although there was no significant difference between filipinos (7.7%), thais (8.3%) and indonesians (12.5% ... | 2004 | 14586642 |
| a prospective study of diet, lifestyle, and genetic factors and the risk of cancer in khon kaen province, northeast thailand: description of the cohort. | cohort studies are the preferred design in observational epidemiology, but few involving the general population have been performed in asia, and most concern affluent urban populations. the khon kaen study has recruited about 25,000 subjects, aged mainly 35-64, from villages in the relatively underdeveloped north-east of thailand. all subjects underwent simple physical examination, completed an interviewer-administered questionnaire (including sections on lifestyle, habits, and diet) and donated ... | 2005 | 16235989 |
| the role of helminth infections in carcinogenesis. | this review examines the significant literature on the role of helminth infections in carcinogenesis. both parasitic infections and cancer have complex natural histories and long latent periods during which numerous exogenous and endogenous factors interact to obfuscate causality. although only two helminths, schistosoma haematobium and opisthorchis viverrini, have been proven to be definitely carcinogenic to humans, others have been implicated in facilitating malignant transformation. the known ... | 2007 | 18063098 |
| characterization of cysteine proteases from the carcinogenic liver fluke, opisthorchis viverrini. | protease activities in extracts of opisthorchis viverrini were investigated using gelatin zymography and fluorogenic peptide substrates. using gelatin-impregnated x-ray film, 2 microg of o. viverrini excretory-secretory products (ov-es) and adult somatic extract (ov-se) showed proteolytic activity. zymography of both o. viverrini extracts revealed bands at approximately 30 kda. using fluorogenic peptide substrates, the majority of o. viverrini activity was determined to be cathepsin l-like cyste ... | 2008 | 18092178 |
| opisthorchis viverrini: efficacy and tegumental alterations following administration of tribendimidine in vivo and in vitro. | the tegumental changes in adult opisthorchis viverrini induced by tribendimidine were analyzed by scanning electron microscopy (sem). we exposed o. viverrini to tribendimidine at a concentration of 10 microg/ml for 4 h. in addition, hamsters were treated with a single 400 mg/kg oral dose of tribendimidine and flukes were recovered from the bile ducts 24, 48, 72, and 96 h post-treatment. sem analysis of the flukes incubated in vitro showed only mild damage of the tegument. twenty-four hours post- ... | 2008 | 18172689 |
| opisthorchis viverrini: the carcinogenic human liver fluke. | opisthorchiasis caused by opisthorchis viverrini remains a major public health problem in many parts of southeast asia, including thailand, lao pdr, vietnam and cambodia. the infection is associated with a number of hepatobiliary diseases, including cholangitis, obstructive jaundice, hepatomegaly, cholecystitis and cholelithiasis. multi-factorial etiology of cholangiocarcinoma, mechanical damage, parasite secretions, and immunopathology may enhance cholangiocarcinogenesis. moreover, both experim ... | 2008 | 18205254 |
| detection of opportunistic and non-opportunistic intestinal parasites and liver flukes in hiv-positive and hiv-negative subjects. | we assessed the frequency and distribution of infection with opportunistic and non-opportunistic intestinal parasites and the liver fluke, opisthorchis viverrini, in hiv-seropositive and hiv-seronegative subjects. age- and sex-matched hiv-seropositive (n = 78) and hiv-seronegative patients (n = 78) from two hospitals in khon kaen province, thailand, participated in this study from november 1998 to august 2000. these subjects were divided according to the presence of diarrhea and cd4 counts. a si ... | 2005 | 16295534 |
| biliary parasites: diagnostic and therapeutic strategies. | parasitic infections of the biliary tract are a common cause of biliary obstruction in endemic areas. this article focuses on primary biliary parasites: ascaris lumbricoides, clonorchis sinensis, opisthorchis viverrini, opisthorchis felineus, dicrocoelium dendriticum, fasciola hepatica, and fasciola gigantica. tropical and subtropical countries have the highest incidence and prevalence of these infections. diagnosis is made primarily through direct microscopic examination of eggs in the stool, d ... | 2008 | 18321435 |
| clonorchis sinensis and opisthorchis viverrini: development of a mitochondrial-based multiplex pcr for their identification and discrimination. | we report a single, one-step pcr approach for detection and discrimination of clonorchis sinensis and opisthorchis viverrini in different life-stage forms (adults, metacercariae, and eggs) from fish intermediate hosts and from infected patients. primers designed for species-specific pcr, amplifying portions of the mitochondrial (mt) genome, were also suitable for a multiplex pcr. the latter was a single, one-step reaction under high stringency conditions, using simultaneously 2 pairs of primers ... | 2006 | 16310774 |
| opisthorchis viverrini: influence of maternal infection in hamsters on offspring infected with homologous parasite and their igg antibody response. | we investigated the influence in hamsters of a maternal opisthorchis viverrini infection on their offspring infected with homologous parasites and the kinetics of the o. viverrini-specific igg antibody responses. no significant difference (p > 0.05) was found in the specific igg antibody response and the number of o. viverrini eggs per gram feces (epg) between infected offspring from infected mothers and infected offspring from uninfected mothers. a significant difference (p < 0.05) of epg per w ... | 2006 | 16472806 |
| oxidative and nitrative stress in opisthorchis viverrini-infected hamsters: an indirect effect after praziquantel treatment. | praziquantel causes adverse effects after short-term treatment. to examine the mechanism of these effects, we studied the distribution of opisthorchis viverrini antigens and the expression of inducible nitric oxide synthase (inos), nuclear factor-kappab (nf-kappab), and antioxidant enzymes in o. viverrini-infected hamsters during short-term praziquantel treatment. praziquantel-induced dispersion of parasite antigens produced a recruitment of inflammatory cells. nf-kappab and inos mrna expression ... | 2008 | 18385350 |
| cholangiocarcinoma: lessons from thailand. | to present the background of liver fluke-associated cholangiocarcinoma in thailand focusing on recent epidemiological data and pathogenesis of this bile duct cancer. | 2008 | 18408464 |
| opisthorchiasis in thailand: review and current status. | opisthorchiasis caused by opisthorchis viverrini (o. viverrini) remains a major public health problem in many parts of southeast asia including thailand, lao pdr, vietnam and cambodia. the infection is associated with a number of hepatobiliary diseases, including cholangitis, obstructive jaundice, hepatomegaly, cholecystitis, chole-lithiasis and cholangiocarcinoma. the liver fluke infection was induced by eating raw or uncooked fish products that is the tradition and popular in the northeastern ... | 2008 | 18416453 |
| mutations of kras and tp53 in a minor proportion of opisthorchis viverrini-associated cholangiocarcinomas in a hamster model. | kras oncogene and tp53 tumor suppressor gene have been known as common genes involving in many cancers including cholangiocarcinoma (ccc). activation of these genes could lead to uncontrolled proliferation and cancer ultimately. the aim of this study was to investigate mutation of kras exon 1 and tp53 exon 5-8 in opisthorchis viverrini (ov)-induced cholangiocarcinoma (cca) in a hamster model. | 2008 | 18439086 |
| development and evaluation of a polymerase chain reaction (pcr) assay for the detection of opisthorchis viverrini in fish. | human opisthorchiais caused by the liver fluke opisthorchis viverrini is a major fish-borne trematode infection endemic in the southeast asian countries. the infection is acquired through consumption of raw fish harboring metacercariae of o. viverrini. owing to potential risk of transmission of opisthorchiasis through fish trade, rapid and reliable detection methods have gained importance to ensure food safety. in the study described here, we report successful development and evaluation of a pol ... | 2008 | 18495082 |
| pathobiology of opisthorchiasis: an update. | opisthorchis viverrini infection is associated with several hepatobiliary diseases including cholangitis, obstructive jaundice, hepatomegaly, cholecystitis and cholelithiasis. pathological consequences of o. viverrini infection occur mainly in the liver, extrahepatic bile ducts, gall bladder and kidney. these pathologies have been described in both humans and experimental animals. moreover, both experimental and epidemiological evidence strongly implicate the liver fluke infection in the etiolog ... | 2003 | 14611875 |
| comparative proteomic analysis of juvenile and adult liver fluke, opisthorchis viverrini. | we used comparative two-dimensional gel electrophoresis to highlight proteins that are differentially expressed in the maturation stage of the parasite opisthorchis viverrini (ov). the proteins differentially expressed in the juvenile/adult forms of the parasite are thought to be important for survival and pathogenesis. we used a nonlinear gradient ph ranged 3-10 strips for isoelectric focusing to resolve soluble proteins from four different maturation periods of ov from 1 week juvenile to 4 wee ... | 2003 | 14611878 |
| opisthorchis viverrini and opisthorchiasis: the 21st century review. proceedings of the congress of opisthorchiasis and cholangiocarcinoma. may 28-30, 2002. khon kaen, thailand. | | 2003 | 14763464 |
| asparaginyl endopeptidase from the carcinogenic liver fluke, opisthorchis viverrini, and its potential for serodiagnosis. | to isolate and characterize an asparaginyl endopeptidase from the carcinogenic liver fluke, opisthorchis viverrini, and evaluate its expression profile, biochemical activity, and potential as an immunodiagnostic antigen. | 2008 | 18619888 |
| high excretion of etheno adducts in liver fluke-infected patients: protection by praziquantel against dna damage. | chronic infection by opisthorchis viverrini (ov) is a strong risk factor for developing cholangiocarcinoma (cca). to clarify the involvement of oxidative stress and lipid peroxidation (lpo)-derived dna damage, the excretion of lpo-derived etheno dna adducts was measured in urine samples collected from healthy volunteers and ov-infected thai subjects. 1,n(6)-etheno-2'-deoxyadenosine (epsilonda) and 3,n(4)-etheno-2'-deoxycytidine (epsilondc) levels were quantified by immunoprecipitation/high-perfo ... | 2008 | 18628417 |
| the identification of a clonorchis sinensis gene encoding an antigenic egg protein. | the cdna library of clonorchis sinensis was screened for genes encoding antigenic proteins by using sera from clonorchiasis patients. a gene of 888 bp encoding a 28-kda protein (cs28) was cloned and found to contain a high percentage of glycine (20%), tyrosine (11%), and lysine (11%). the amino acid sequence of cs28 showed 60% homology with the vitelline b precursor protein of opisthorchis viverrini and of 33% homology with the vitelline b1 and b2 proteins of fasciola hepatica. a strong positive ... | 2004 | 15616856 |
| inos-dependent dna damage via nf-kappab expression in hamsters infected with opisthorchis viverrini and its suppression by the antihelminthic drug praziquantel. | inflammation-mediated dna damage triggered by opisthorchis viverrini (ov) infection is a major risk factor of cholangiocarcinoma (cca). we have recently reported that nitrative and oxidative dna damage participates in cca development caused by repeated infection with ov [pinlaor et al., carcinogenesis 2004; 25:1535-42]. therefore, to clarify the preventive effect of the antihelminthic drug praziquantel against cholangiocarcinogenesis, we assessed the effect of this drug on nitrative and oxidativ ... | 2006 | 16570287 |
| genetic comparison of liver flukes, clonorchis sinensis and opisthorchis viverrini, based on rdna and mtdna gene sequences. | to clarify the genetic relationships between clonorchis sinensis and opisthorchis viverrini, patterns of inter-/intraspecific polymorphism were compared for four markers with nuclear ribosomal dna (rdna) and mitochondrial dna (mtdna) in liver flukes c. sinensis from korea (kimhae) and china (shenyang and nanning) and o. viverrini from laos (savannakhet). intra- and interspecific variations in the 18s, its2, and 28s rdna and mitochondrial cytochrome c oxidase subunit i (mtcoi) of mtdna gene seque ... | 2007 | 16902795 |
| genetic variation in opisthorchis viverrini (trematoda: opisthorchiidae) from northeast thailand and laos pdr based on random amplified polymorphic dna analyses. | genetic variation in opisthorchis viverrini adults originating from different locations in northeast thailand and laos, people's democratic republic (pdr), was examined using random amplified polymorphic dna (rapd) analyses. in an initial analysis, the genomic dna of one fluke from each of ten localities was amplified using 15 random primers (10-mers); however, genetic variation among o. viverrini specimens was detected reliably for only four primers. a more detailed rapd analysis using these fo ... | 2007 | 17016722 |
| opisthorchis viverrini: gene expression profiling of carcinogenic adult liver fluke worms using 5' sage. | opisthorchis viverrini is the only liver fluke that has been proved to be associated with cholangiocarcinoma (cca). however, the mechanisms by which o. viverrini participates in the carcinogenesis of cca are still unclear. to understand the biology and host-parasite interaction related to o. viverrini infection, gene expression profiling of this parasite is required. here, we constructed the first 5' serial analysis of gene expression (5' sage) library of the adult o. viverrini and matched the d ... | 2008 | 18786530 |
| opisthorchis viverrini: detection by polymerase chain reaction (pcr) in human stool samples. | a polymerase chain reaction (pcr) assay was evaluated for detection of opisthorchis viverrini eggs in the stool specimens of light and heavily infected individuals in khon kaen province of thailand. a total of 75 fecal specimens were analyzed by pcr following dna extraction. all the microscopically positive samples were positive by pcr, while 23 of 30 (76.6%) microscopically negative samples were also pcr positive. the sensitivity of the assay was 5 eggs/g of stool. this method is potentially us ... | 2008 | 18805413 |
| function association of enhanced expressed genes between opisthorchis viverrini and non opisthorchis viverrini-related cholangiocarcinoma. | | 2008 | 18808295 |
| opisthorchis viverrini: an underestimated parasite in world health. | infection with opisthorchis viverrini and its associated cholangiocarcinoma (cca) is an underestimated problem in the mekong region of southeast asia, despite the widespread use of praziquantel and health education measures for parasite control. although data from cambodia, laos and vietnam are rare, data from thailand often show wide-ranging variability in epidemiological parameters, including human morbidity and the prevalence and incidence of cca. the recent discovery of high levels of popula ... | 2008 | 18930439 |
| mitochondrial dna sequence variation among geographical isolates of opisthorchis viverrini in thailand and lao pdr, and phylogenetic relationships with other trematodes. | the present study compared the genetic variation among 14 different geographical isolates of opisthorchis viverrini sensu lato from thailand and lao pdr using sequence data for 2 mitochondrial dna genes, the subunit 1 of nadh dehydrogenase gene (nad1) and cytochrome c oxidase gene (cox1). four different nad1 haplotypes were detected among isolates, all of which were identical at the amino acid sequence level. nucleotide sequence variation among 14 isolates ranged from 0 to 0.3% for nad1. two dif ... | 2008 | 18937886 |
| discrimination of o. viverrini, c. sinensis, h. pumilio and h. taichui using nuclear dna-based pcr targeting ribosomal dna its regions. | small liver and minute intestinal flukes are highly prevalent in southeast asia, and in mixed infections, their eggs are difficult to differentiate morphologically in fecal samples. pcr assays targeting the its regions in ribosomal dna were designed to identify and differentiate species. the pcr amplicons of opisthorchis viverrini, clonorchis sinensis, haplorchis pumilio, and haplorchis taichui were 800, 820, 1250, and 930 bp for the its1 region, and 380, 390, 380, and 530 bp for its2, respectiv ... | 2009 | 18952037 |
| three cases of intestinal capillariasis in lao people's democratic republic. | capillaria philippinensis is a rare zoonotic intestinal parasite that emerged in the 1960s. the outcome of intestinal capillariasis may be fatal if untreated in due time. we report three cases of intestinal capillariasis in lao people's democratic republic (lao pdr). the three patients were unrelated previously healthy young men (24, 26, and 27 years of age) with no underlying disease or immune depression. they had chronic diarrhea, abdominal pain, edema, and severe weight loss. two of them acqu ... | 2008 | 18981514 |
| ov-apr-1, an aspartic protease from the carcinogenic liver fluke, opisthorchis viverrini: functional expression, immunolocalization and subsite specificity. | the human liver fluke opisthorchis viverrini is endemic in thailand, laos and cambodia where long standing infection is associated with cancer of the bile ducts, cholangiocarcinoma. here we describe a cathepsin d-like aspartic protease from the gut and other tissues in o. viverrini. phylogenetic analysis indicated that ov-apr-1 is cathepsin d-like, conforming with clan aa, family a1 of the merops classification. ov-apr-1 is expressed in the gut of the mature hermaphroditic parasite, in the repro ... | 2009 | 18996218 |
| diversity of human intestinal helminthiasis in lao pdr. | food-borne trematodiasis is an emerging public health problem, including in lao pdr. we investigated the diversity of intestinal helminthes and polyparasitism in patients with hepatobiliary or intestinal symptoms in hospital and community-based surveys. stool samples from 232 individuals aged >or=15 years were examined by the kato-katz method (three samples) and a formalin ethyl-acetate concentration technique (one sample). opisthorchis viverrini and minute intestinal flukes (mif) were common, w ... | 2009 | 19038411 |
| animal models for opisthorchis viverrini infection. | we investigated the utility of various animal models for the study of opisthorchiasis in humans and its common sequel of cholangiocarcinoma (cca). rats, mice, gerbils, and hamsters were infected with opisthorchis viverrini metacercariae. worms from the infected animal hosts were recovered from livers and counts made of eggs per gram of feces. worms were observed in and recovered from hamsters and gerbils but not rats and mice. the recovered worms from the infected gerbils were larger and more ph ... | 2009 | 19050927 |
| rare human infection with the trematode echinochasmus japonicus in lao pdr. | food-borne trematodiases are often neglected although millions of people are affected and these zoonotic diseases are emerging in some parts of the world. humans acquire an infection through consumption of the second intermediate hosts (e.g. freshwater fish) that harbour the metacercariae of the parasite. here, we report the first three cases of echinochasmus japonicus infection in lao pdr. adult e. japonicus flukes were recovered from a woman (age: 37 years) and two men (age: 42 and 75 years) f ... | 2009 | 19059359 |
| resistance of cholangiocarcinoma cells to parthenolide-induced apoptosis by the excretory-secretory products of clonorchis sinensis. | infection by clonorchis sinensis, the chinese or oriental liver fluke, is a significant risk factor for the development of cholangiocarcinoma, a human epithelial carcinoma of the intrahepatic bile duct. parthenolide is a sesquiterpene lactone that has strong anticancer properties and is also known to induce apoptosis in cholangiocarcinoma cells. many investigators have reported that excretory-secretory (es) products of c. sinensis as well as opisthorchis viverrini promote the development of chol ... | 2009 | 19066964 |
| clonorchis sinensis: molecular cloning and functional expression of a novel cytosolic glutathione transferase. | glutathione transferases (gsts) represent a large family of enzymes. in the high throughput sequencing of the cdna library constructed from the adult stage of clonorchis sinensis (cs), we isolate another cdna clone encoding a novel cytosolic gst enzyme. to discriminate with our former reported csgst, we designated this gst as csgst1. this new cdna contains 744 bp with a putative open reading frame of 213 amino acids. the deduced amino acid sequence exhibits 88% identity to opisthorchis viverrini ... | 2007 | 17048005 |
| a new pcr-based approach indicates the range of clonorchis sinensis now extends to central thailand. | differentiation of the fish-borne trematodes belonging to the opisthorchiidae, heterophyidae and lecithodendriidae is important from a clinical and epidemiological perspective, yet it is impossible to do using conventional coprological techniques, as the eggs are morphologically similar. epidemiological investigation therefore currently relies on morphological examination of adult worms following expulsion chemotherapy. a pcr test capable of amplifying a segment of the internal transcribed space ... | 2009 | 19156191 |
| time profiles of the expression of metalloproteinases, tissue inhibitors of metalloproteases, cytokines and collagens in hamsters infected with opisthorchis viverrini with special reference to peribiliary fibrosis and liver injury. | the liver fluke opisthorchis viverrini is endemic in southeastern asia, and causes cholangiocarcinoma and liver fibrosis. we investigated the time profile of the expression of matrix metalloproteinases (mmps) and tissue inhibitors of mmps (timps) in relation to peribiliary fibrosis in o. viverrini-infected hamsters. hepatic mrna expression of mmps, timps, cytokines and collagens i and iii was assessed by quantitative reverse transcription-pcr. zymography and immunohistochemistry were also used t ... | 2009 | 19168069 |
| intestinal parasitic infections in hill-tribe schoolchildren in chiang mai, northern thailand. | we surveyed intestinal parasitic infections in hill-tribe schoolchildren residing permanently in chiang mai province. the positive rate, of 403 stool specimens examined using the formalin-ether sedimentation technique, was 48.9%. no significant difference between male (50.8%) and female (47.1%) students was found for the infection. the most common protozoa was entamoeba coli (40.9%), followed by giardia lamblia (14.9%). the most common helminth was hookworm (13.4%), followed by ascaris lumbricoi ... | 2003 | 19230577 |
| studies on concomitant antigens of bithynia funiculata for detection of antibody to opisthorchis viverrini: effect of different centrifugal speeds on antigen preparation. | antigens derived from somatic extracts of bithynia funiculata, an intermediate snail host of o. viverrini, have been demonstrated to be highly heterogeneous in molecular weight (mw). these antigens have been suggested to be of potential use for serodiagnosis. in this study, b. funiculata somatic antigens were extracted using five different centrifugal speeds, namely 10,000 (c1); 20,000 (c2); 30,000 (c3); 40,000 (c4) and 50,000 (c5) rpm, with the aim of removing some non-specific antigens and det ... | 2003 | 19230581 |
| pcr diagnosis of infections with different species of opisthorchiidae using a rapid clean-up procedure for stool samples and specific primers. | infections with the opisthorchiid liver flukes clonorchis sinensis, opisthorchis viverrini, and opisthorchis felineus cause serious health problems in endemic areas of southeast asia and countries of the former soviet union. chronic infections--even with low worm burdens--may lead to the development of fatal cholangiocarcinoma and related symptoms. a more sensitive diagnosis is needed since the tiny eggs of the worms are often not seen in microscopic examinations of stool samples, especially in ... | 2007 | 17061114 |
| cathepsin f cysteine protease of the human liver fluke, opisthorchis viverrini. | the liver fluke opisthorchis viverrini is classified as a class i carcinogen due to the association between cholangiocarcinoma and chronic o. viverrini infection. during its feeding activity within the bile duct, the parasite secretes several cathepsin f cysteine proteases that may induce or contribute to the pathologies associated with hepatobiliary abnormalities. | 2009 | 19308250 |
| dose-response relationships and tegumental surface alterations in opisthorchis viverrini following treatment with mefloquine in vivo and in vitro. | the treatment and control of opisthorchiasis relies on a single drug, praziquantel; hence, there is a need to develop novel opisthorchicidal drugs. we investigated the in vitro and in vivo activity of the antimalarial mefloquine against opisthorchis viverrini. hamsters infected with o. viverrini for 2 weeks (juvenile infections) and 4 weeks (adult infections) were treated orally with single 200-400-mg/kg oral mefloquine. worm burden reductions were assessed against untreated control hamsters. wo ... | 2009 | 19308455 |
| field investigation of bithynia funiculata, intermediate host of opisthorchis viverrini in northern thailand. | a survey of freshwater snail, bithynia funiculata, was conducted in four locations, doi saket, mueang, saraphi and mae rim districts of chiang mai province, northern thailand, between june and october, during the rainy season of 2004. a total of 2,240 snails was collected and classified into 7 families and 15 genera; of which 352 b. funiculata were obtained. b. funiculata was found most abundant in july and september. the infection rate of trematode cercariae in b. funiculata was 9.6% (19/352), ... | 2006 | 17121291 |
| [experimental study progress on tribendimidine, artemether and artesunate against clonorchis sinensis and other trematodes]. | currently praziquantel is one of the major drugs used in treatment of schistosomiasis and other trematode infections. recent experimental studies indicate that a new anthelmintic, tribendimidine, is used in the treatment of intestinal nematodes, also possesses effect against several species of trematodes including clonorchis sinensis, opisthorchis viverrini and echinostoma caproni. tribendimidine is even more effective against c. sinensis in rats that a single 300 mg/kg oral dose cures almost al ... | 2009 | 19459505 |
| impact of temporal changes and host factors on the genetic structure of a population of opisthorchis viverrini sensu lato in khon kaen province (thailand). | the population genetics of 317 individual opisthorchis viverrini from khon kaen province thailand, from 4 different years and 4 cyprinid fish species was examined using multilocus enzyme electrophoresis of enolase (enol), phosphoglucomutase (pgm) and triose phosphate isomerase (tpi). allele and genotype frequencies for enol and pgm were consistent irrespective of year or host species. no heterozygote deficiency was detected for enol. significant heterozygote deficiencies were detected in 3 of 4 ... | 2009 | 19523258 |
| genetic markers for the identification and characterization of opisthorchis viverrini, a medically important food borne trematode in southeast asia. | the liver fluke, opisthorchis viverrini, is one of the major food borne trematodes in southeast asia, where infection causes hepatobiliary disease and subsequent development of cholangiocarcinoma. in thailand, o. viverrini is most prevalent in the northeast where there is marked regional variation in the rate of infection in humans at provincial, district and village levels. to date, the roles of genetic variation of o. viverrini on this observed variability in infection, transmission and associ ... | 2006 | 17166476 |
| risk factors for cholangiocarcinoma in khon kaen, thailand: a nested case-control study. | the present nested case-control study within the khon kaen cohort study was conducted to assess risk factors for cholangiocarcinoma (cca) development. cases were 108 subjects with proven cca, by ultrasound at least, and controls also numbered 108, matched by sex, age (not more than 3 years difference) and period of recruitment to the cohort (not more than 3 months difference). a questionnaire was constructed based on that employed for the khon kaen cohort study recruitment. mcnemar's chi-square ... | 2009 | 19537893 |
| cholangiocarcinoma in experimental hamsters with long-standing opisthorchis viverrini infection. | liver fluke infection of opisthorchis viverrini (o. viverrini) is closely associated with several hepatobiliary diseases including cholangiocarcinoma (cca), but no reports have described these diseases in chronic and long-standing experimental opisthorchiasis in hamsters more than 10 months of age. a longer period of infection could induce different pathological lesions. to prove the hypothesis, we therefore sequentially investigated histological changes of the hepatobiliary system in 4 groups o ... | 2009 | 19537899 |
| incidence and risk factors of opisthorchis viverrini infections in a rural community in thailand. | opisthorchis viverrini infection is one of the major public health problems in thailand. although information about transmission of this parasite to humans is well established, infections are still prevalent in the northern and northeastern thailand. thus, prospective epidemiologic information is needed for developing more effective public health interventions. a cohort study to identify incidence and risk factors of o. viverrini infection was conducted in a rural community in thailand during de ... | 2009 | 19556581 |
| alterations of gene expression of rb pathway in opisthorchis viverrini infection-induced cholangiocarcinoma. | opisthorchiasis has the significant relationship with the high prevalence of cholangiocarcinoma (cca; a bile duct cancer) in the endemic areas in southeast asia. to reveal the molecular mechanism of the tumorigenesis induced by opisthorchis viverrini infection, the present study investigated the kinetic expression of rb pathway genes, including rb1, p16(ink4), cyclin d1, and cdk4, during the development of opisthorchiasis-associated cca in hamster model. the results of quantitative real-time pol ... | 2009 | 19582476 |
| parasitic infestation and nutritional status among schoolchildren in vientiane, lao pdr. | to investigate the prevalence of intestinal parasitic infestation among school-aged children residing in vientiane capital city; and to assess the impact of its infestation on nutrition status of those children. | 2007 | 17854455 |
| turmeric reduces inflammatory cells in hamster opisthorchiasis. | the curcumin compound from turmeric is effective in the treatment of many inflammatory diseases. the aim of our present study was to evaluate the efficacy of turmeric on reducing the histopathological changes of hamster opisthorchiasis. hamsters were infected with opisthorchis viverrini and then administered turmeric. using light microscopic observation, liver function tests for alanine transaminase (alt), alkaline phosphatase, and direct bilirubin were investigated. the resulting histopathologi ... | 2009 | 19633869 |
| biological variation within opisthorchis viverrini sensu lato in thailand and lao pdr. | previous molecular genetic analyses indicate that opisthorchis viverrini is a complex of at least 2 cryptic species in thailand and the lao people's democratic republic (pdr). opisthorchis viverrini sensu lato (s.l.) populations can be divided into 6 genetic groups, which correlate with 5 different river wetlands systems. the present study examines the infectivity, growth, fecundity, and body size of o. viverrini s.l. isolates from these systems. worm recovery was lowest in the songkhram river, ... | 2009 | 19658451 |
| advanced periductal fibrosis from infection with the carcinogenic human liver fluke opisthorchis viverrini correlates with elevated levels of interleukin-6. | more than 750 million people are at risk of infection with foodborne liver flukes. opisthorchis viverrini is considered among the most important of these parasites, due to its strong association with cholangiocarcinoma (cca). o. viverrini infection results in a chronic inflammatory challenge to the host, which can lead to advanced, pathogen-specific disease sequelae including obstructive jaundice, hepatomegaly, cholecystitis, as well as cca. however, before disease sequelae are apparent, importa ... | 2009 | 19676135 |
| the elisa-based detection of anti-opisthorchis viverrini igg and igg4 in samples of human urine and serum from an endemic area of north-eastern thailand. | the levels of correlation between the number of opisthorchis viverrini eggs excreted in the faeces and levels of anti-opisthorchis igg and igg(4) in the serum and urine (as indicated by absorbances in elisa) have recently been evaluated in north-eastern thailand. the 225 subjects investigated in detail, all of whom came from an endemic village in chaiyaphum province, were selected on the basis of the numbers of o. viverrini eggs that they were excreting. elisa based on a crude antigen extract of ... | 2007 | 17877877 |
| high prevalence of haplorchis taichui, phaneropsolus molenkampi, and other helminth infections among people in khammouane province, lao pdr. | the prevalence of liver and intestinal helminth infections, including opisthorchis, haplorchis, phaneropsolus, hookworms, enterobius, and taenia, was surveyed in khammouane province, lao pdr. fecal specimens were collected from 1,242 people (590 men and 652 women) in 3 mekong riverside villages and were examined by the kato-katz thick smear technique. the overall helminth egg positive rate was 81.1%. the positive rate for small trematode eggs, including opisthorchis viverrini, heterophyids, and ... | 2009 | 19724697 |
| curcumin reduces oxidative and nitrative dna damage through balancing of oxidant-antioxidant status in hamsters infected with opisthorchis viverrini. | opisthorchis viverrini (ov) infection is endemic in northeastern thailand. we have previously reported that ov infection induces oxidative and nitrative dna damage via chronic inflammation, which contributes to the disease and cholangiocarcinogenesis. here, we examined the effect of curcumin, an antioxidant, on pathogenesis in ov-infected hamsters. dna lesions were detected by double immunofluorescence and the hepatic expression of oxidant-generating and antioxidant genes was assessed by quantit ... | 2009 | 19753608 |
| a novel nuclear marker, pm-int9, for phylogenetic studies of opisthorchis felineus, opisthorchis viverrini, and clonorchis sinensis (opisthorchiidae, trematoda). | opisthorchis felineus, o. viverrini, and clonorchis sinensis, the trematodes of the family opisthorchiidae, are important human parasites. two previous studies (kang et al. parasitol int 57:191-197, 2008; katokhin et al. dokl biochem biophys 421:214-217, 2008) have provided evidence using ribosomal and mitochondrial sequences that o. viverrini, o. felineus, and c. sinensis are closely related. we developed a novel nuclear marker, pm-int9, which included the ninth intron of the paramyosin gene an ... | 2009 | 19777262 |
| a granulin-like growth factor secreted by the carcinogenic liver fluke, opisthorchis viverrini, promotes proliferation of host cells. | the human liver fluke, opisthorchis viverrini, infects millions of people throughout south-east asia and is a major cause of cholangiocarcinoma, or cancer of the bile ducts. the mechanisms by which chronic infection with o. viverrini results in cholangiocarcinogenesis are multi-factorial, but one such mechanism is the secretion of parasite proteins with mitogenic properties into the bile ducts, driving cell proliferation and creating a tumorigenic environment. using a proteomic approach, we iden ... | 2009 | 19816559 |
| helminths and malignancy. | it has been estimated that chronic infections with viruses, bacteria and parasites contribute to 17.8% of the global burden of cancer, although only a relatively small proportion of the infection-related cancers can be attributed to helminth infections. these are important because of the high number of people who are exposed or infected worldwide. carcinogenesis associated with helminth infections is a complex process, which may involve several different mechanisms, but chronic inflammation is a ... | 2009 | 19825108 |
| rapid molecular detection of opisthorchis viverrini in human fecal samples by real-time polymerase chain reaction. | real-time fluorescence resonance energy transfer (fret) polymerase chain reaction (pcr) supplemented with melting curve analysis is a highly sensitive and fast method offering a high throughput. we report the development of a real-time fret pcr for molecular detection of opisthorchis viverrini in human fecal samples. the diagnostic sensitivity, specificity, accuracy, and positive and negative predictive values of this method were 97.5%, 100%, 98.9%, 100%, and 98.2%, respectively. the sensitivity ... | 2009 | 19861632 |
| improvement of pcr for detection of opisthorchis viverrini dna in human stool samples. | opisthorchis viverrini is an important food-borne trematode in southeast asia. the infection causes significant morbidity in terms of hepatobiliary diseases and cholangiocarcinoma. the aim of this study was to improve the sensitivity of the pcr-based diagnosis of o. viverrini infection. a new fecal dna extraction protocol for the detection of o. viverrini dna using cetyltrimethyl-ammoniumbromide to remove pcr inhibitor was used and compared with the commercial stool kit method. the sensitivity o ... | 2008 | 18003810 |
| formation of 8-nitroguanine, a nitrative dna lesion, in inflammation-related carcinogenesis and its significance. | chronic infection and inflammation contribute to a substantial part of environmental carcinogenesis. recently, it has been estimated that chronic inflammation accounts for approximately 25% of cancer cases. various infectious diseases and physical, chemical, and immunological factors participate in inflammation-related carcinogenesis. under inflammatory conditions, reactive oxygen and nitrogen species, which are generated from inflammatory and epithelial cells, may play an important role in carc ... | 2009 | 19921494 |
| elucidating the transcriptome of fasciola hepatica - a key to fundamental and biotechnological discoveries for a neglected parasite. | liver flukes of animals are parasitic flatworms (platyhelminthes: digenea) of major socioeconomic importance in many countries. key representatives, such as fasciola hepatica and f. gigantica, cause "liver fluke disease" (= fascioliasis), which is of major animal health significance worldwide. in particular, f. hepatica is a leading cause of production losses to the livestock (mainly sheep and cattle) and meat industries due to clinical disease, reduced weight gain and milk production, and death ... | 2010 | 20006979 |
| apoptosis-related gene expression in hamster opisthorchiasis post praziquantel treatment. | the aim of this study was to investigate the apoptosis-related gene expression in hamster opisthorchiasis after praziquantel treatment. hamsters were infected with opisthorchis viverrini metacercariae then treated with praziquantel. the expression of apoptosis-related genes [i.e., apoptosis gene bcl-2-associated protein x (bax), caspase 9, p53, and protein kinase b (pkb)] was detected by real-time reverse transcription polymerase chain reaction. histopathological analyses of liver tissues were s ... | 2008 | 18058134 |
| the secreted and surface proteomes of the adult stage of the carcinogenic human liver fluke opisthorchis viverrini. | infection with the human liver fluke, opisthorchis viverrini, is a serious public health problem in thailand, laos and nearby locations in southeast asia. both experimental and epidemiological evidence strongly implicate liver fluke infection in the etiology of one of the liver cancer subtypes, cholangiocarcinoma (cca). to identify parasite proteins critical for liver fluke survival and the etiology of cca, offgel electrophoresis and multiple reaction monitoring were employed to characterize 300 ... | 2010 | 20049860 |
| lipid peroxidation and etheno dna adducts in white blood cells of liver fluke-infected patients: protection by plasma alpha-tocopherol and praziquantel. | chronic infection by the liver fluke opisthorchis viverrini is a strong risk factor for cholangiocarcinoma. to clarify the involvement of oxidative stress and lipid peroxidation-derived dna damage, etheno (epsilon)-dna adducts (epsilonda, epsilondc) in wbc and plasma alpha-tocopherol were measured in samples collected from o. viverrini-infected thai patients (n = 50) and healthy noninfected volunteers (n = 20). epsilonda and epsilondc levels were three to five times higher (p < 0.001) in infecte ... | 2010 | 20056652 |
| molecular identification and phylogenetic analysis of nuclear rdna sequences among three opisthorchid liver fluke species (opisthorchiidae: trematoda). | in this study, we describe the development of a fast and accurate molecular identification system for human-associated liver fluke species (opisthorchis viverrini, opisthorchis felineus, and clonorchis sinensis) using the pcr-rflp analysis of the 18s-its1-5.8s nuclear ribosomal dna region. based on sequence variation in the target rdna region, we selected three species-specific restriction enzymes within the its1 regions, generating different restriction profiles among the species: muni for o. v ... | 2008 | 18276183 |
| opisthorchis viverrini: evaluation of 28 kda glutathione s-transferase as diagnostic tool in human opisthorchiasis. | the liver fluke opisthorchis viverrini is the agent of human opisthorchiasis in thailand with a high prevalence observed in the rural population of north and northeastern regions of the country. a focus of research has therefore been the development of diagnostic tools to indicate infection by this parasite. in the present study, a 28 kda glutathione s-transferase of o. viverrini (ov28gst), which is found in the excretion/secretion product of the parasite, was evaluated for its application in di ... | 2010 | 20096257 |
| prevalence and potential risk factors of rhinitis and atopic eczema among schoolchildren in vientiane capital, lao pdr: isaac questionnaire. | in 1998, an epidemiological study on asthma and allergic diseases using isaac questionnaire in laos was first conducted in the recommended schools located in vientiane capital showing that the prevalence of rhinoconjunctivitis and atopic eczema were 23.7% and 7.1% among children aged 13-14 year-old, respectively. this study aimed to reassess the prevalence of rhinoconjunctivitis and atopic eczema using the same isaac questionnaire by employing random sampling method and to identify the potential ... | 2008 | 20103927 |
| involvement of mmp-9 in peribiliary fibrosis and cholangiocarcinogenesis via rac1-dependent dna damage in a hamster model. | peribiliary fibrosis caused by chronic infection with opisthorchis viverrini (ov) is a risk factor of cholangiocarcinoma (cca) in northeastern thailand. matrix metalloproteinases (mmps) are enzymes capable of degrading and remodeling the extracellular matrix in the process of fibrosis and carcinogenesis. we examined mmps expression and their role in fibrogenesis and cholangiocarcinogenesis in hamsters treated with ov and n-nitrosodimethylamine (ndma). we assessed the time profiles of mmps, induc ... | 2010 | 20162672 |
| monophyly of opisthorchis viverrini populations in the lower mekong basin, using mitochondrial dna nad1 gene as the marker. | the liver fluke, opisthorchis viverrini, causes serious public-health problems in the lower mekong basin. this study aimed to clarify whether o. viverrini populations may be genetically divided into sub-specific taxa. we collected 6 populations of o. viverrini from different places in cambodia, lao pdr, and thailand, along both sides of the mekong river, and analyzed the population structure of these using the mitochondrial nad1 gene as a marker. the results of the dna polymorphism measurements, ... | 2010 | 20197110 |
| taqman real-time pcr assay for specific detection of opisthorchis viverrini dna in thai patients with hepatocellular carcinoma and cholangiocarcinoma. | the aim of this study was to develop taqman real-time pcr assay that detected opisthorchis viverrini dna from 18 normal and 18 tumor tissue specimens from thai patients with hepatocellular carcinoma (hcc) and cholangiocarcinoma (cca), who underwent liver resection from october 2005 to may 2006. control liver specimens were seven non-primary liver cancers. a conserved probe representing 100% sequence homology was used as a reference for o. viverrini-specific probe. five of six tumors (83%) and al ... | 2008 | 18329641 |
| urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine in patients with parasite infection and effect of antiparasitic drug in relation to cholangiocarcinogenesis. | parasite infection of opisthorchis viverrini is a major risk factor for cholangiocarcinoma. our previous immunohistochemical studies showed that o. viverrini infection induced oxidative dna lesions in the bile duct epithelium during cholangiocarcinoma development. the current study assessed the levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg), an oxidative dna lesion, in the urine and leukocytes of o. viverrini-infected subjects and cholangiocarcinoma patients. forty-nine o. viverrini-inf ... | 2008 | 18349269 |
| food-borne trematode infections of humans in the united states of america. | this review examines the literature on imported (allochthonous) and local (autochthonous) cases of food-borne trematode (fbt) infections in the united states of america (usa) from 1890 to 2009. most of the literature is concerned with imported cases of the opisthorchiids clonorchis sinensis and opisthorchis viverrini. these flukes cause serious pathology in the liver and biliary system of humans. chronic cases may induce liver (hepatocarcinoma) and bile duct (cholangiocarcinoma) cancers in human ... | 2010 | 20352454 |
| reduction of periductal fibrosis in liver fluke-infected hamsters after long-term curcumin treatment. | chronic infection with the liver fluke, opisthorchis viverrini, induces advanced periductal fibrosis and is a relative risk factor for cholangiocarcinoma in southeastern asia. we examined the reducing effect of curcumin on hepatobiliary fibrosis using o. viverrini-infected hamsters supplemented with dietary 1% curcumin (w/w) as an animal model. the expression profile of matrix metalloproteinases (mmps) and tissue inhibitors of mmps (timps), cytokines, and collagens was assessed in relation to li ... | 2010 | 20420820 |
| detection of opisthorchis viverrini in infected bithynid snails by real-time fluorescence resonance energy transfer pcr-based method and melting curve analysis. | a real-time fluorescence resonance energy transfer (fret) pcr combined with melting curve analysis was developed for the detection of opisthorchis viverrini in experimentally infected bithynid snails, its first intermediate hosts. the test is based on the fluorescence melting curve analysis of a hybrid between an amplicon from the pov-a6-specific probe sequence, a 162-bp repeated sequence specific to o. viverrini and specific fluorophore-labeled probes. the real-time fret pcr could detect as lit ... | 2008 | 18512076 |
| up-regulation of annexin a2 in cholangiocarcinoma caused by opisthorchis viverrini and its implication as a prognostic marker. | cholangiocarcinoma (cca), or cancer of the bile ducts, is primarily associated with infection with the liver fluke opisthorchis viverrini in northeast thailand. the disease is associated with late presentation, poses challenges for diagnosis and has a high mortality rate--features that highlight the need for tumor markers. at present, there are no specific tumor markers that can indicate the early stages and status of cca. proteomic analysis of the proteins expressed on the surface of tumor cell ... | 2010 | 20493868 |
| proteomic identification of peroxiredoxin 6 for host defence against opisthorchis viverrini infection. | opisthorchis viverrini infection causes opisthorchiasis and is a risk factor for cholangiocarcinoma via chronic inflammation. to investigate the mechanism of o. viverrini -induced liver disease, we applied a proteomic approach to examine alterations in hepatic protein levels in o. viverrini -infected hamsters. two-dimensional gel electrophoresis (2de) revealed that o. viverrini infection induced upregulation (1.5- to 4.3-fold) of 25 proteins and downregulation (1.5 to 2.5-fold) of 24 proteins co ... | 2010 | 20500660 |
| chronic bacterial and parasitic infections and cancer: a review. | a relatively underestimated facet of infectious diseases is the association of chronic bacterial and parasitic infections with cancer development. therefore, we sought to evaluate the evidence regarding the association of such infections with the development of malignancy, excluding the overwhelming evidence of the association of helicobacter pylori and cancer. | 2010 | 20539059 |