| clonorchis sinensis and opisthorchis viverrini: development of a mitochondrial-based multiplex pcr for their identification and discrimination. | we report a single, one-step pcr approach for detection and discrimination of clonorchis sinensis and opisthorchis viverrini in different life-stage forms (adults, metacercariae, and eggs) from fish intermediate hosts and from infected patients. primers designed for species-specific pcr, amplifying portions of the mitochondrial (mt) genome, were also suitable for a multiplex pcr. the latter was a single, one-step reaction under high stringency conditions, using simultaneously 2 pairs of primers ... | 2006 | 16310774 |
| prevalence of blastocystis hominis infection in asymptomatic individuals from bangkok, thailand. | fresh stool examination was performed from 2,230 participants who enrolled in annual check-up programs of the faculty of medical technology, mahidol university in 1999-2000 and 2004. in this study, blastocystis hominis infection was diagnosed by culturing in jones' media. a total of 21% of fecal specimens (in 1999-2000) and 13.7% (in 2004) were positive for b. hominis. the vacuolated form was the predominant form found in culture solution after 48 hours of incubation. the distribution of infecti ... | 2005 | 16438173 |
| methylene blue staining method for identification of opisthorchis viverrini egg. | methylene blue staining method was used to distinguish o. viverrini eggs from haplorchis taichui and prosthodendrium molenkampi eggs. all eggs were obtained from dissected adult worms, fixed in 10% formalin, and stained with methylene blue prior to light microscopy observation. the distinct musk-melon-like texture of the o. viverini eggshell surface and the thread-like texture of h. taichui eggshell surface were recognized, while p. molenkampi eggs showed a smooth eggshell. we also evaluated the ... | 2005 | 16438191 |
| emergence of opisthorchis viverrini cercariae from naturally infected bithynia (digoniostoma) siamensis goniomphalos. | under natural conditions, the emergence of opisthorchis viverrini cercariae from naturally infected bithynia (digoniostoma) siamensis goniomphalos showed diurnal periodicity, peaking between 8:00-10:00 am. the cercariae did not emerge during darkness, but low-intensity light could induce a release. cercariae shedded from each field infected b.(d.) s. goniomphalos was recorded daily. the maximum output from one snail was 1,728 cercariae in a day. the total cercarial output from all five infected ... | 2005 | 16438207 |
| opisthorchis viverrini: influence of maternal infection in hamsters on offspring infected with homologous parasite and their igg antibody response. | we investigated the influence in hamsters of a maternal opisthorchis viverrini infection on their offspring infected with homologous parasites and the kinetics of the o. viverrini-specific igg antibody responses. no significant difference (p > 0.05) was found in the specific igg antibody response and the number of o. viverrini eggs per gram feces (epg) between infected offspring from infected mothers and infected offspring from uninfected mothers. a significant difference (p < 0.05) of epg per w ... | 2006 | 16472806 |
| altered gene expression in opisthorchis viverrini-associated cholangiocarcinoma in hamster model. | cholangiocarcinoma (cca) induced by liver fluke (opisthorchis viverrini, ov) infection is one of the most common and serious disease in northeast thailand. to elucidate the molecular mechanism of cholangiocarcinogenesis induced by ov infection, we employed a hamster model of cca induced by ov and n-nitrosodimethylamine and analyzed candidate genes involved in cca using fluorescence differential display-pcr. of 149 differentially amplified bands we identified, the upregulation of 23 transcripts a ... | 2006 | 16550611 |
| glycobiological study of adult opisthorchis viverrini: characterization of n-linked oligosaccharides. | | 2006 | 16567007 |
| inos-dependent dna damage via nf-kappab expression in hamsters infected with opisthorchis viverrini and its suppression by the antihelminthic drug praziquantel. | inflammation-mediated dna damage triggered by opisthorchis viverrini (ov) infection is a major risk factor of cholangiocarcinoma (cca). we have recently reported that nitrative and oxidative dna damage participates in cca development caused by repeated infection with ov [pinlaor et al., carcinogenesis 2004; 25:1535-42]. therefore, to clarify the preventive effect of the antihelminthic drug praziquantel against cholangiocarcinogenesis, we assessed the effect of this drug on nitrative and oxidativ ... | 2006 | 16570287 |
| oxidative and nitrative dna damage in animals and patients with inflammatory diseases in relation to inflammation-related carcinogenesis. | infection and chronic inflammation are proposed to contribute to carcinogenesis through inflammation-related mechanisms. infection with hepatitis c virus, helicobacter pylori and the liver fluke, opisthorchis viverrini (ov), are important risk factors for hepatocellular carcinoma (hcc), gastric cancer and cholangiocarcinoma, respectively. inflammatory bowel diseases (ibds) and oral diseases, such as oral lichen planus (olp) and leukoplakia, are associated with colon carcinogenesis and oral squam ... | 2006 | 16606333 |
| 8-nitroguanine, a product of nitrative dna damage caused by reactive nitrogen species: formation, occurrence, and implications in inflammation and carcinogenesis. | the authors review studies on 8-nitroguanine (8-no(2)-g) formed by reactions of guanine, guanosine, and 2 - deoxyguanosine, either free or in dna or rnawith reactive nitrogen species (rns) generated from peroxynitrite, the myeloperoxidase-h(2)o(2)-nitrite system, and others. use of antibodies against 8-no(2)-g has revealed increased formation of 8-no(2)-g in various pathological conditions, including rna virus-induced pneumonia in mice, intrahepatic bile ducts of hamsters infected with the liver ... | 2006 | 16771693 |
| gene expression profiling defined pathways correlated with fibroblast cell proliferation induced by opisthorchis viverrini excretory/secretory product. | to investigate the mechanism of fibroblast cell proliferation stimulated by the opisthorchis viverrini excretory/secretory (es) product. | 2006 | 16773716 |
| epidemiology of opisthorchis viverrini in a rural district of southern lao pdr. | in lao pdr, detailed investigations on opisthorchis viverrini are scarce. the objective was to document epidemiological features of o. viverrini infections in a highly endemic district. a random sample was selected (13 villages, 15 households/village, all household members aged >6 months). clinical examinations, short interviews and stool examinations (kato-katz technique) were performed. fish samples were dissected for the presence of metacercaria. in total, 814 persons were enrolled (median ag ... | 2007 | 16828134 |
| opisthorchis viverrini: identification of a glycine-tyrosine rich eggshell protein and its potential as a diagnostic tool for human opisthorchiasis. | a cdna encoding a novel eggshell protein (ovesp) with high-glycine (49.2%) and -tyrosine (27.8%) content was cloned from the human liver fluke opisthorchis viverrini. in the adult parasite, the rna products of the ovesp gene are limited to the vitelline follicles. they have a size of 800 nucleotides and are already present in 2-week-old juveniles. immune sera of hamsters, experimentally infected, and humans, naturally infected with o. viverrini, detect bacterially expressed recombinant ovesp (ro ... | 2006 | 16876169 |
| genetic comparison of liver flukes, clonorchis sinensis and opisthorchis viverrini, based on rdna and mtdna gene sequences. | to clarify the genetic relationships between clonorchis sinensis and opisthorchis viverrini, patterns of inter-/intraspecific polymorphism were compared for four markers with nuclear ribosomal dna (rdna) and mitochondrial dna (mtdna) in liver flukes c. sinensis from korea (kimhae) and china (shenyang and nanning) and o. viverrini from laos (savannakhet). intra- and interspecific variations in the 18s, its2, and 28s rdna and mitochondrial cytochrome c oxidase subunit i (mtcoi) of mtdna gene seque ... | 2007 | 16902795 |
| effect of artesunate and artemether against clonorchis sinensis and opisthorchis viverrini in rodent models. | food-borne trematodiasis is an emerging public health problem and there is a need to develop novel treatment options. we examined the in vivo efficacy of single oral doses of artesunate and artemether administered to rodents experimentally infected with either clonorchis sinensis or opisthorchis viverrini. rats infected with adult c. sinensis were administered artesunate or artemether at doses of 75, 150 or 300 mg/kg. hamsters infected with adult o. viverrini were administered artesunate or arte ... | 2006 | 16973335 |
| comparison of gene expression profiles between opisthorchis viverrini and non-opisthorchis viverrini associated human intrahepatic cholangiocarcinoma. | intrahepatic cholangiocarcinoma (icc) is the second most common primary cancer in the liver, and its incidence is highest in the northeastern part of thailand. iccs in this region are known to be associated with infection with liver flukes, particularly opisthorchis viverrini (ov), as well as nitrosamines from food. to clarify molecular mechanisms of icc associated with or without liver flukes, we analyzed gene expression profiles of ov-associated iccs from 20 thai patients and compared their pr ... | 2006 | 17006947 |
| genetic variation in opisthorchis viverrini (trematoda: opisthorchiidae) from northeast thailand and laos pdr based on random amplified polymorphic dna analyses. | genetic variation in opisthorchis viverrini adults originating from different locations in northeast thailand and laos, people's democratic republic (pdr), was examined using random amplified polymorphic dna (rapd) analyses. in an initial analysis, the genomic dna of one fluke from each of ten localities was amplified using 15 random primers (10-mers); however, genetic variation among o. viverrini specimens was detected reliably for only four primers. a more detailed rapd analysis using these fo ... | 2007 | 17016722 |
| clonorchis sinensis: molecular cloning and functional expression of a novel cytosolic glutathione transferase. | glutathione transferases (gsts) represent a large family of enzymes. in the high throughput sequencing of the cdna library constructed from the adult stage of clonorchis sinensis (cs), we isolate another cdna clone encoding a novel cytosolic gst enzyme. to discriminate with our former reported csgst, we designated this gst as csgst1. this new cdna contains 744 bp with a putative open reading frame of 213 amino acids. the deduced amino acid sequence exhibits 88% identity to opisthorchis viverrini ... | 2007 | 17048005 |
| ultrasound screening for opisthorchis viverrini-associated cholangiocarcinomas: experience in an endemic area. | in thailand, liver cancer is the most common malignancy in males and the third most common among females. in the northeast region, cholangiocarcinoma (chca) is the prevalent type, with opisthorchis viverrini (ov), an endemic liver fluke, being considered the cause. we evaluated the role of ultrasound (u/s) for cholangiocarcinoma screening as part of a larger cohort to characterize the linkage between liver fluke infestation and chca in khon kaen (northeast thailand). most people (77%) had normal ... | 2006 | 17059338 |
| pcr diagnosis of infections with different species of opisthorchiidae using a rapid clean-up procedure for stool samples and specific primers. | infections with the opisthorchiid liver flukes clonorchis sinensis, opisthorchis viverrini, and opisthorchis felineus cause serious health problems in endemic areas of southeast asia and countries of the former soviet union. chronic infections--even with low worm burdens--may lead to the development of fatal cholangiocarcinoma and related symptoms. a more sensitive diagnosis is needed since the tiny eggs of the worms are often not seen in microscopic examinations of stool samples, especially in ... | 2007 | 17061114 |
| clonorchis sinensis: development and evaluation of a nested polymerase chain reaction (pcr) assay. | clonorchis sinensis is a fish-borne trematode endemic to east asia, which infects over 35 million people globally. in the study described here, we developed a nested polymerase chain reaction (pcr) method for the specific and reliable detection of c. sinensis. the primers designed from the nucleotide sequence data derived in this study were evaluated for their specificity and sensitivity for the detection of c. sinensis. the specific amplification products were obtained only with c. sinensis and ... | 2007 | 17067580 |
| field investigation of bithynia funiculata, intermediate host of opisthorchis viverrini in northern thailand. | a survey of freshwater snail, bithynia funiculata, was conducted in four locations, doi saket, mueang, saraphi and mae rim districts of chiang mai province, northern thailand, between june and october, during the rainy season of 2004. a total of 2,240 snails was collected and classified into 7 families and 15 genera; of which 352 b. funiculata were obtained. b. funiculata was found most abundant in july and september. the infection rate of trematode cercariae in b. funiculata was 9.6% (19/352), ... | 2006 | 17121291 |
| genetic markers for the identification and characterization of opisthorchis viverrini, a medically important food borne trematode in southeast asia. | the liver fluke, opisthorchis viverrini, is one of the major food borne trematodes in southeast asia, where infection causes hepatobiliary disease and subsequent development of cholangiocarcinoma. in thailand, o. viverrini is most prevalent in the northeast where there is marked regional variation in the rate of infection in humans at provincial, district and village levels. to date, the roles of genetic variation of o. viverrini on this observed variability in infection, transmission and associ ... | 2006 | 17166476 |
| evaluation of the in vivo activity of tribendimidine against schistosoma mansoni, fasciola hepatica, clonorchis sinensis, and opisthorchis viverrini. | we examined the in vivo activity of tribendimidine against selected trematodes. a single 150-mg/kg dose of tribendimidine achieved a 99.1% reduction of clonorchis sinensis in rats. a 400-mg/kg dose of tribendimidine reduced opisthorchis viverrini in hamsters by 95.7%. high doses of tribendimidine showed no activity against schistosoma mansoni and fasciola hepatica. | 2007 | 17194822 |
| cytokine expression in hamsters experimentally infected with opisthorchis viverrini. | the cytokine mrna expression of il-12, ifn-gamma, tgf-beta, il-4, and il-10 were investigated in spleen, liver and mesenteric lymph nodes (mln) in hamsters experimentally infected with opisthorchis viverrini. animals were infected with 5, 25 or 100 metacercariae (mc) and examined by rt-pcr and real-time pcr at 2 weeks, 2 and 6 months after infection. the cytokine expression was compared using hprt. the il-12 was significantly expressed at 2 weeks in the liver of the 5- and 25-mc-infected groups. ... | 2007 | 17266743 |
| evidence of a species complex within the food-borne trematode opisthorchis viverrini and possible co-evolution with their first intermediate hosts. | the food-borne trematodes, opisthorchis viverrini, o. felineus and clonorchis sinensis, have long been recognized as the cause of major human health problems, with an estimated 40 million infected persons. of the three species of liver fluke, only o. viverrini is classified as a type 1 carcinogen because of its role as an initiator of chronic inflammation and the subsequent development of cholangiocarcinoma. at present, there are no techniques for the early diagnosis of cholangiocarcinoma and it ... | 2007 | 17275001 |
| epidemiology of food-borne trematodes and other parasite infections in a fishing community on the nam ngum reservoir, lao pdr. | food-borne trematodes (fbt) are important causes of parasitic infections in many asian countries. parasitological surveys in xai udom, a small fishing community on the nam ngum reservoir, lao people's democratic republic, revealed an overall parasitic infection rate in may 1999 of 68.8% (n = 173) and in december 1999 of 65.9% (n = 261). the liver fluke, opisthorchis viverrini accounted for most of the infections (prevalences of 53.8% and 42.1%, during the first and second surveys, respectively). ... | 2006 | 17333758 |
| early stage biliary and intrahepatic migration of opisthorchis viverrini in the golden hamster. | in thailand, infection with the liver fluke opisthorchis viverrini is a serious health problem, with over 8.6 million human infections each year. early stage biliary intrahepatic migration and infection of o. viverrini in the syrian golden hamster were used to study the growth and development of this fluke. fifty metacercariae of o. viverrini were introduced into each hamster by gastric intubation. worms were found to migrate rapidly from the stomach to the gall bladder and hepatic duct, where t ... | 2007 | 17381865 |
| increased tff1 trefoil protein expression in opisthorchis viverrini-associated cholangiocarcinoma is important for invasive promotion. | aims: cholangiocarcinoma (cca) is a poor prognosis cancer that presents with metastatic disease. this cancer expresses muc5ac, a mucin which normally co-expresses with trefoil factor family 1 (tff1) protein. tff1 is a signalling protein that can activate epithelial cell invasion and has been considered as a metastasis stimulating agent. the aim of this study was to determine the co-expression of tff1 and muc5ac in cca tissues and examine the activity of tff1 for stimulating the invasive property ... | 2007 | 17397518 |
| parasitic infestations of the biliary tract. | parasitic infestations of the biliary tract are a common cause of biliary obstruction in tropical countries and can lead to such serious complications as cholangitis and cholangiocarcinoma. endoscopic therapy has helped in the management of biliary complications caused by these parasites. ascaris lumbricoides organisms, which normally reside in the jejunum, are actively motile and can invade the papilla, thus migrating into the bile duct and causing biliary obstruction. endoscopic retrograde cho ... | 2007 | 17418062 |
| parasitic diseases of the biliary tract. | objective: parasites residing in the biliary tree include clonorchis sinensis, opisthorchis viverrini, opisthorchis felineus, and fasciola hepatica. they are willowy, leaf-like, flat flukes dwelling in the bile ducts and gallbladder. human ascarides, ascaris lumbricoides, dwelling in the small intestine, inadvertently migrate into the bile ducts and cause biliary obstruction. the purpose of this article is to illustrate typical imaging findings of liver fluke infection and biliary ascariasis. co ... | 2007 | 17515382 |
| the potential usefulness of the modified kato thick smear technique in the detection of intestinal sarcocystosis during field surveys. | a total of 479 stool specimens were collected from rural communities of ubon ratchathani province, thailand and examined by two techniques: the modified kato thick smear and the direct smear. the prevalence of opisthorchis viverrini (14.8%), hookworm (10.2%), sarcocystis spp (4.6%), taenia spp (2.9%), strongyloides stercoralis (2.1%), giardia lamblia (1.2%), echinostoma spp (0.6%), ascaris lumbricoides (0.4%), entamoeba histolytica (0.2%), chilomastix mesnili (0.2%) and endolimax nana (0.2%) wer ... | 2007 | 17539271 |
| enzyme markers to identify and characterize opisthorchis viverrini in thailand and lao pdr. | we conducted an allozyme electrophoretic study to explore potential enzyme markers to distinguish opisthorchis viverrini in thailand and lao pdr. twenty-eight enzymes encoding presumptive 32 loci were established. the enzymes glucose-6-phosphate dehydrogenase and pyruvate kinase were diagnostic between two geographically separate isolates from thailand. twelve enzymes, ie, aconitate hydratase, aldolase, creatine kinase, enolase, esterases, fumarate hydratase, aspartate aminotransferase, glucose- ... | 2006 | 17547051 |
| dna quantities and qualities from various stages of some trematodes using optical and hat-rapd methods. | the aim of this experiment was to minimize dna quantity and quality for detection by optical (spectrophotometer at 260 nm and 280 nm) and hat-rapd methods. total dna from different stages, adult, metacercaria and eggs of 6 trematode species were isolated for analysis. in this experiment, the adult trematodes were classified into 3 groups by size: small, haplorchis taichui and stellantchasmus falcatus; medium, opisthorchis viverrini and ganeo tigrinus; and large, paramphistomum epiclitum and fisc ... | 2006 | 17547055 |
| discrimination of opisthorchis viverrini from haplorchis taichui using coi sequence marker. | this study aimed to discriminate infections of two common fish-borne trematodes in thailand, opisthorchis viverrini from haplorchis taichui, based on mitochondrial cytochrome c oxidase subunit i (coi) gene. designed primers (coi-ov-hap f&r primers) amplified partial coi fragments of o. viverrini and h. taichui with high sensitivity in different developmental stages (adult, metacercaria, and egg). polymerase chain reaction (pcr) amplicons were generated with low genomic dna concentration ( approx ... | 2007 | 17574199 |
| gene discovery for the carcinogenic human liver fluke, opisthorchis viverrini. | cholangiocarcinoma (cca)--cancer of the bile ducts--is associated with chronic infection with the liver fluke, opisthorchis viverrini. despite being the only eukaryote that is designated as a 'class i carcinogen' by the international agency for research on cancer, little is known about its genome. | 2007 | 17587442 |
| apoptosis-related gene expressions in hamsters re-infected with opisthorchis viverrini and re-treated with praziquantel. | our objective was to reveal whether host immune response in hamster opisthorchiasis post-praziquantel treatment could induce apoptotic cell death in inflammatory cells. we, therefore, investigated apoptosis-related gene expression in hamsters re-infected with opisthorchis viverrini (ov) and re-treated with praziquantel. hamsters were re-infected with ov metacercariae then re-treated with praziquantel. the expression of apoptosis-related genes (i.e. apoptosis gene bcl-2 associated protein x [bax] ... | 2007 | 17851691 |
| parasitic infestation and nutritional status among schoolchildren in vientiane, lao pdr. | to investigate the prevalence of intestinal parasitic infestation among school-aged children residing in vientiane capital city; and to assess the impact of its infestation on nutrition status of those children. | 2007 | 17854455 |
| high prevalence of liver and intestinal fluke infections among residents of savannakhet province in laos. | the prevalence of liver and intestinal fluke infections was surveyed on residents of savannakhet province, laos. fecal specimens were collected from a total of 981 residents in 4 mekong riverside villages and examined by the kato-katz thick smear technique. the results revealed that the overall helminth egg positive rate was 84.2%, and the positive rate for small trematode eggs, including opisthorchis viverrini, heterophyids, or lecithodendriids, was 67.1%. to obtain adult flukes, 38 small trema ... | 2007 | 17876167 |
| the elisa-based detection of anti-opisthorchis viverrini igg and igg4 in samples of human urine and serum from an endemic area of north-eastern thailand. | the levels of correlation between the number of opisthorchis viverrini eggs excreted in the faeces and levels of anti-opisthorchis igg and igg(4) in the serum and urine (as indicated by absorbances in elisa) have recently been evaluated in north-eastern thailand. the 225 subjects investigated in detail, all of whom came from an endemic village in chaiyaphum province, were selected on the basis of the numbers of o. viverrini eggs that they were excreting. elisa based on a crude antigen extract of ... | 2007 | 17877877 |
| biliary parasitic diseases including clonorchiasis, opisthorchiasis and fascioliasis. | parasitic infection of the biliary tree is caused by liver flukes, namely clonorchis sinensis and opisthorchis viverrini. these flukes reside in the peripheral small bile ducts of the liver and produce chronic inflammation of the bile duct, bile duct dilatation, mechanical obstruction, and bile duct wall thickening. on imaging, peripheral small intrahepatic bile ducts are dilated, but the large bile ducts and extrahepatic bile ducts are not dilated or slightly dilated. there is no visible caused ... | 2008 | 17934771 |
| artemisinins and synthetic trioxolanes in the treatment of helminth infections. | helminthiases, including schistosomiasis and food-borne trematodiasis, affect millions of people. just a few drugs are used for the treatment and control of these diseases. we review recent in-vitro and in-vivo observations with the artemisinins and synthetic trioxolanes against major trematode infections, update clinical findings, and discuss the potential impact that artemisinin-based combination therapy might have on trematode infections in settings where malaria and helminthiases are co-ende ... | 2007 | 17975411 |
| improvement of pcr for detection of opisthorchis viverrini dna in human stool samples. | opisthorchis viverrini is an important food-borne trematode in southeast asia. the infection causes significant morbidity in terms of hepatobiliary diseases and cholangiocarcinoma. the aim of this study was to improve the sensitivity of the pcr-based diagnosis of o. viverrini infection. a new fecal dna extraction protocol for the detection of o. viverrini dna using cetyltrimethyl-ammoniumbromide to remove pcr inhibitor was used and compared with the commercial stool kit method. the sensitivity o ... | 2008 | 18003810 |
| apoptosis-related gene expression in hamster opisthorchiasis post praziquantel treatment. | the aim of this study was to investigate the apoptosis-related gene expression in hamster opisthorchiasis after praziquantel treatment. hamsters were infected with opisthorchis viverrini metacercariae then treated with praziquantel. the expression of apoptosis-related genes [i.e., apoptosis gene bcl-2-associated protein x (bax), caspase 9, p53, and protein kinase b (pkb)] was detected by real-time reverse transcription polymerase chain reaction. histopathological analyses of liver tissues were s ... | 2008 | 18058134 |
| the role of helminth infections in carcinogenesis. | this review examines the significant literature on the role of helminth infections in carcinogenesis. both parasitic infections and cancer have complex natural histories and long latent periods during which numerous exogenous and endogenous factors interact to obfuscate causality. although only two helminths, schistosoma haematobium and opisthorchis viverrini, have been proven to be definitely carcinogenic to humans, others have been implicated in facilitating malignant transformation. the known ... | 2007 | 18063098 |
| characterization of cysteine proteases from the carcinogenic liver fluke, opisthorchis viverrini. | protease activities in extracts of opisthorchis viverrini were investigated using gelatin zymography and fluorogenic peptide substrates. using gelatin-impregnated x-ray film, 2 microg of o. viverrini excretory-secretory products (ov-es) and adult somatic extract (ov-se) showed proteolytic activity. zymography of both o. viverrini extracts revealed bands at approximately 30 kda. using fluorogenic peptide substrates, the majority of o. viverrini activity was determined to be cathepsin l-like cyste ... | 2008 | 18092178 |
| opisthorchis viverrini: efficacy and tegumental alterations following administration of tribendimidine in vivo and in vitro. | the tegumental changes in adult opisthorchis viverrini induced by tribendimidine were analyzed by scanning electron microscopy (sem). we exposed o. viverrini to tribendimidine at a concentration of 10 microg/ml for 4 h. in addition, hamsters were treated with a single 400 mg/kg oral dose of tribendimidine and flukes were recovered from the bile ducts 24, 48, 72, and 96 h post-treatment. sem analysis of the flukes incubated in vitro showed only mild damage of the tegument. twenty-four hours post- ... | 2008 | 18172689 |
| opisthorchis viverrini: the carcinogenic human liver fluke. | opisthorchiasis caused by opisthorchis viverrini remains a major public health problem in many parts of southeast asia, including thailand, lao pdr, vietnam and cambodia. the infection is associated with a number of hepatobiliary diseases, including cholangitis, obstructive jaundice, hepatomegaly, cholecystitis and cholelithiasis. multi-factorial etiology of cholangiocarcinoma, mechanical damage, parasite secretions, and immunopathology may enhance cholangiocarcinogenesis. moreover, both experim ... | 2008 | 18205254 |
| molecular identification and phylogenetic analysis of nuclear rdna sequences among three opisthorchid liver fluke species (opisthorchiidae: trematoda). | in this study, we describe the development of a fast and accurate molecular identification system for human-associated liver fluke species (opisthorchis viverrini, opisthorchis felineus, and clonorchis sinensis) using the pcr-rflp analysis of the 18s-its1-5.8s nuclear ribosomal dna region. based on sequence variation in the target rdna region, we selected three species-specific restriction enzymes within the its1 regions, generating different restriction profiles among the species: muni for o. v ... | 2008 | 18276183 |
| biliary parasites: diagnostic and therapeutic strategies. | parasitic infections of the biliary tract are a common cause of biliary obstruction in endemic areas. this article focuses on primary biliary parasites: ascaris lumbricoides, clonorchis sinensis, opisthorchis viverrini, opisthorchis felineus, dicrocoelium dendriticum, fasciola hepatica, and fasciola gigantica. tropical and subtropical countries have the highest incidence and prevalence of these infections. diagnosis is made primarily through direct microscopic examination of eggs in the stool, d ... | 2008 | 18321435 |
| taqman real-time pcr assay for specific detection of opisthorchis viverrini dna in thai patients with hepatocellular carcinoma and cholangiocarcinoma. | the aim of this study was to develop taqman real-time pcr assay that detected opisthorchis viverrini dna from 18 normal and 18 tumor tissue specimens from thai patients with hepatocellular carcinoma (hcc) and cholangiocarcinoma (cca), who underwent liver resection from october 2005 to may 2006. control liver specimens were seven non-primary liver cancers. a conserved probe representing 100% sequence homology was used as a reference for o. viverrini-specific probe. five of six tumors (83%) and al ... | 2008 | 18329641 |
| urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine in patients with parasite infection and effect of antiparasitic drug in relation to cholangiocarcinogenesis. | parasite infection of opisthorchis viverrini is a major risk factor for cholangiocarcinoma. our previous immunohistochemical studies showed that o. viverrini infection induced oxidative dna lesions in the bile duct epithelium during cholangiocarcinoma development. the current study assessed the levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg), an oxidative dna lesion, in the urine and leukocytes of o. viverrini-infected subjects and cholangiocarcinoma patients. forty-nine o. viverrini-inf ... | 2008 | 18349269 |
| oxidative and nitrative stress in opisthorchis viverrini-infected hamsters: an indirect effect after praziquantel treatment. | praziquantel causes adverse effects after short-term treatment. to examine the mechanism of these effects, we studied the distribution of opisthorchis viverrini antigens and the expression of inducible nitric oxide synthase (inos), nuclear factor-kappab (nf-kappab), and antioxidant enzymes in o. viverrini-infected hamsters during short-term praziquantel treatment. praziquantel-induced dispersion of parasite antigens produced a recruitment of inflammatory cells. nf-kappab and inos mrna expression ... | 2008 | 18385350 |
| cholangiocarcinoma: lessons from thailand. | to present the background of liver fluke-associated cholangiocarcinoma in thailand focusing on recent epidemiological data and pathogenesis of this bile duct cancer. | 2008 | 18408464 |
| opisthorchiasis in thailand: review and current status. | opisthorchiasis caused by opisthorchis viverrini (o. viverrini) remains a major public health problem in many parts of southeast asia including thailand, lao pdr, vietnam and cambodia. the infection is associated with a number of hepatobiliary diseases, including cholangitis, obstructive jaundice, hepatomegaly, cholecystitis, chole-lithiasis and cholangiocarcinoma. the liver fluke infection was induced by eating raw or uncooked fish products that is the tradition and popular in the northeastern ... | 2008 | 18416453 |
| mutations of kras and tp53 in a minor proportion of opisthorchis viverrini-associated cholangiocarcinomas in a hamster model. | kras oncogene and tp53 tumor suppressor gene have been known as common genes involving in many cancers including cholangiocarcinoma (ccc). activation of these genes could lead to uncontrolled proliferation and cancer ultimately. the aim of this study was to investigate mutation of kras exon 1 and tp53 exon 5-8 in opisthorchis viverrini (ov)-induced cholangiocarcinoma (cca) in a hamster model. | 2008 | 18439086 |
| development and evaluation of a polymerase chain reaction (pcr) assay for the detection of opisthorchis viverrini in fish. | human opisthorchiais caused by the liver fluke opisthorchis viverrini is a major fish-borne trematode infection endemic in the southeast asian countries. the infection is acquired through consumption of raw fish harboring metacercariae of o. viverrini. owing to potential risk of transmission of opisthorchiasis through fish trade, rapid and reliable detection methods have gained importance to ensure food safety. in the study described here, we report successful development and evaluation of a pol ... | 2008 | 18495082 |
| detection of opisthorchis viverrini in infected bithynid snails by real-time fluorescence resonance energy transfer pcr-based method and melting curve analysis. | a real-time fluorescence resonance energy transfer (fret) pcr combined with melting curve analysis was developed for the detection of opisthorchis viverrini in experimentally infected bithynid snails, its first intermediate hosts. the test is based on the fluorescence melting curve analysis of a hybrid between an amplicon from the pov-a6-specific probe sequence, a 162-bp repeated sequence specific to o. viverrini and specific fluorophore-labeled probes. the real-time fret pcr could detect as lit ... | 2008 | 18512076 |
| characterization of the antioxidant enzyme, thioredoxin peroxidase, from the carcinogenic human liver fluke, opisthorchis viverrini. | the human liver fluke, opisthorchis viverrini, induces inflammation of the hepatobiliary system. despite being constantly exposed to inimical oxygen radicals released from inflammatory cells, the parasite survives for many years. the mechanisms by which it avoids oxidative damage are unknown. in this study, thioredoxin peroxidase (tpx), a member of the peroxiredoxin superfamily, was cloned from an o. viverrini cdna library. o. viverrini tpx cdna encoded a polypeptide of 212 amino acid residues, ... | 2008 | 18538872 |
| baseline health situation of communities affected by the nam theun 2 hydroelectric project in central lao pdr and indicators for monitoring. | hydroelectric projects offer opportunities for infrastructure development and economic growth; yet, if not well designed, implemented and operated, they have the potential to negatively affect the health and well-being of local and distant downstream communities. remote rural populations are particularly vulnerable to the sudden influx of men, materials and money, and associated population mixing that accompany project construction phases. two large-scale baseline health surveys, carried out in ... | 2008 | 18569149 |
| asparaginyl endopeptidase from the carcinogenic liver fluke, opisthorchis viverrini, and its potential for serodiagnosis. | to isolate and characterize an asparaginyl endopeptidase from the carcinogenic liver fluke, opisthorchis viverrini, and evaluate its expression profile, biochemical activity, and potential as an immunodiagnostic antigen. | 2008 | 18619888 |
| high excretion of etheno adducts in liver fluke-infected patients: protection by praziquantel against dna damage. | chronic infection by opisthorchis viverrini (ov) is a strong risk factor for developing cholangiocarcinoma (cca). to clarify the involvement of oxidative stress and lipid peroxidation (lpo)-derived dna damage, the excretion of lpo-derived etheno dna adducts was measured in urine samples collected from healthy volunteers and ov-infected thai subjects. 1,n(6)-etheno-2'-deoxyadenosine (epsilonda) and 3,n(4)-etheno-2'-deoxycytidine (epsilondc) levels were quantified by immunoprecipitation/high-perfo ... | 2008 | 18628417 |
| prevalence, intensity and risk factors for clonorchiasis and possible use of questionnaires to detect individuals at risk in northern vietnam. | a high prevalence of the liver flukes clonorchis sinensis and opisthorchis viverrini has been reported in a number of provinces in vietnam. knowledge about c. sinensis infection gained from thailand over the past decade suggests that the habit of eating raw freshwater fish is a major risk factor for infection. however, further information to confirm this is needed. in the present study 1155 villagers in two communes in northern vietnam were interviewed and their stools were examined for the pres ... | 2008 | 18632126 |
| genetic variation at three enzyme loci within a thailand population of opisthorchis viverrini. | genetic variation among 231 individuals of opisthorchis viverrini from the ban phai district, khon kaen province (thailand) was examined at three polymorphic enzymes: enolase (enol), phosphoglucomutase (pgm), and triose phosphate isomerase (tpi). four alleles were detected for tpi and pgm, whereas only two alleles were detected for enol. the inferred genotype frequencies for both tpi and enol were not significantly different from hardy-weinberg equilibrium. in contrast, the inferred genotype fre ... | 2008 | 18690476 |
| update on hepatobiliary flukes: fascioliasis, opisthorchiasis and clonorchiasis. | hepatobiliary flukes--fasciola, opisthorchis, clonorchis- are a major public health problem in east asia, east europe, africa and latin america. the present review focuses on current knowledge of clinical, diagnostic and treatment aspects caused by hepatobiliary flukes that can be applied to current protocols in endemic areas. | 2008 | 18725803 |
| real-time fluorescence resonance energy transfer pcr with melting curve analysis for the detection of opisthorchis viverrini in fish intermediate hosts. | a real-time fluorescence resonance energy transfer (fret) pcr combined with a melting curve analysis was developed for the detection of opisthorchis viverrini in its fish intermediate host, cyprinoid fishes. real-time fret pcr is based on a fluorescence melting curve analysis of a hybrid between an amplicon generated from a family of repeated dna elements, the pov-a6 specific probe sequence (genbank accession no. s80278), a 162 bp repeated sequence specific to o. viverrini, and specific fluoroph ... | 2008 | 18760538 |
| opisthorchis viverrini: gene expression profiling of carcinogenic adult liver fluke worms using 5' sage. | opisthorchis viverrini is the only liver fluke that has been proved to be associated with cholangiocarcinoma (cca). however, the mechanisms by which o. viverrini participates in the carcinogenesis of cca are still unclear. to understand the biology and host-parasite interaction related to o. viverrini infection, gene expression profiling of this parasite is required. here, we constructed the first 5' serial analysis of gene expression (5' sage) library of the adult o. viverrini and matched the d ... | 2008 | 18786530 |
| opisthorchis viverrini: detection by polymerase chain reaction (pcr) in human stool samples. | a polymerase chain reaction (pcr) assay was evaluated for detection of opisthorchis viverrini eggs in the stool specimens of light and heavily infected individuals in khon kaen province of thailand. a total of 75 fecal specimens were analyzed by pcr following dna extraction. all the microscopically positive samples were positive by pcr, while 23 of 30 (76.6%) microscopically negative samples were also pcr positive. the sensitivity of the assay was 5 eggs/g of stool. this method is potentially us ... | 2008 | 18805413 |
| function association of enhanced expressed genes between opisthorchis viverrini and non opisthorchis viverrini-related cholangiocarcinoma. | | 2008 | 18808295 |
| opisthorchis viverrini: an underestimated parasite in world health. | infection with opisthorchis viverrini and its associated cholangiocarcinoma (cca) is an underestimated problem in the mekong region of southeast asia, despite the widespread use of praziquantel and health education measures for parasite control. although data from cambodia, laos and vietnam are rare, data from thailand often show wide-ranging variability in epidemiological parameters, including human morbidity and the prevalence and incidence of cca. the recent discovery of high levels of popula ... | 2008 | 18930439 |
| mitochondrial dna sequence variation among geographical isolates of opisthorchis viverrini in thailand and lao pdr, and phylogenetic relationships with other trematodes. | the present study compared the genetic variation among 14 different geographical isolates of opisthorchis viverrini sensu lato from thailand and lao pdr using sequence data for 2 mitochondrial dna genes, the subunit 1 of nadh dehydrogenase gene (nad1) and cytochrome c oxidase gene (cox1). four different nad1 haplotypes were detected among isolates, all of which were identical at the amino acid sequence level. nucleotide sequence variation among 14 isolates ranged from 0 to 0.3% for nad1. two dif ... | 2008 | 18937886 |
| discrimination of o. viverrini, c. sinensis, h. pumilio and h. taichui using nuclear dna-based pcr targeting ribosomal dna its regions. | small liver and minute intestinal flukes are highly prevalent in southeast asia, and in mixed infections, their eggs are difficult to differentiate morphologically in fecal samples. pcr assays targeting the its regions in ribosomal dna were designed to identify and differentiate species. the pcr amplicons of opisthorchis viverrini, clonorchis sinensis, haplorchis pumilio, and haplorchis taichui were 800, 820, 1250, and 930 bp for the its1 region, and 380, 390, 380, and 530 bp for its2, respectiv ... | 2009 | 18952037 |
| three cases of intestinal capillariasis in lao people's democratic republic. | capillaria philippinensis is a rare zoonotic intestinal parasite that emerged in the 1960s. the outcome of intestinal capillariasis may be fatal if untreated in due time. we report three cases of intestinal capillariasis in lao people's democratic republic (lao pdr). the three patients were unrelated previously healthy young men (24, 26, and 27 years of age) with no underlying disease or immune depression. they had chronic diarrhea, abdominal pain, edema, and severe weight loss. two of them acqu ... | 2008 | 18981514 |
| ov-apr-1, an aspartic protease from the carcinogenic liver fluke, opisthorchis viverrini: functional expression, immunolocalization and subsite specificity. | the human liver fluke opisthorchis viverrini is endemic in thailand, laos and cambodia where long standing infection is associated with cancer of the bile ducts, cholangiocarcinoma. here we describe a cathepsin d-like aspartic protease from the gut and other tissues in o. viverrini. phylogenetic analysis indicated that ov-apr-1 is cathepsin d-like, conforming with clan aa, family a1 of the merops classification. ov-apr-1 is expressed in the gut of the mature hermaphroditic parasite, in the repro ... | 2009 | 18996218 |
| diversity of human intestinal helminthiasis in lao pdr. | food-borne trematodiasis is an emerging public health problem, including in lao pdr. we investigated the diversity of intestinal helminthes and polyparasitism in patients with hepatobiliary or intestinal symptoms in hospital and community-based surveys. stool samples from 232 individuals aged >or=15 years were examined by the kato-katz method (three samples) and a formalin ethyl-acetate concentration technique (one sample). opisthorchis viverrini and minute intestinal flukes (mif) were common, w ... | 2009 | 19038411 |
| animal models for opisthorchis viverrini infection. | we investigated the utility of various animal models for the study of opisthorchiasis in humans and its common sequel of cholangiocarcinoma (cca). rats, mice, gerbils, and hamsters were infected with opisthorchis viverrini metacercariae. worms from the infected animal hosts were recovered from livers and counts made of eggs per gram of feces. worms were observed in and recovered from hamsters and gerbils but not rats and mice. the recovered worms from the infected gerbils were larger and more ph ... | 2009 | 19050927 |
| rare human infection with the trematode echinochasmus japonicus in lao pdr. | food-borne trematodiases are often neglected although millions of people are affected and these zoonotic diseases are emerging in some parts of the world. humans acquire an infection through consumption of the second intermediate hosts (e.g. freshwater fish) that harbour the metacercariae of the parasite. here, we report the first three cases of echinochasmus japonicus infection in lao pdr. adult e. japonicus flukes were recovered from a woman (age: 37 years) and two men (age: 42 and 75 years) f ... | 2009 | 19059359 |
| resistance of cholangiocarcinoma cells to parthenolide-induced apoptosis by the excretory-secretory products of clonorchis sinensis. | infection by clonorchis sinensis, the chinese or oriental liver fluke, is a significant risk factor for the development of cholangiocarcinoma, a human epithelial carcinoma of the intrahepatic bile duct. parthenolide is a sesquiterpene lactone that has strong anticancer properties and is also known to induce apoptosis in cholangiocarcinoma cells. many investigators have reported that excretory-secretory (es) products of c. sinensis as well as opisthorchis viverrini promote the development of chol ... | 2009 | 19066964 |
| fishborne trematode metacercariae detected in freshwater fish from vientiane municipality and savannakhet province, lao pdr. | freshwater fish from vientiane municipality and savannakhet province, lao pdr were examined by the muscle compression and artificial digestion methods to know the infection status with trematode metacercariae. in the fish from savannakhet, 2 species of metacercariae, opisthorchis viverrini and haplorchis taichui, were detected. o. viverrini metacercariae were found in 6 species of fish, puntius brevis, hampala dispar, esomus metallicus, mystacoleucus marginatus, puntioplites falcifer, and cycloc ... | 2008 | 19127332 |
| a new pcr-based approach indicates the range of clonorchis sinensis now extends to central thailand. | differentiation of the fish-borne trematodes belonging to the opisthorchiidae, heterophyidae and lecithodendriidae is important from a clinical and epidemiological perspective, yet it is impossible to do using conventional coprological techniques, as the eggs are morphologically similar. epidemiological investigation therefore currently relies on morphological examination of adult worms following expulsion chemotherapy. a pcr test capable of amplifying a segment of the internal transcribed space ... | 2009 | 19156191 |
| time profiles of the expression of metalloproteinases, tissue inhibitors of metalloproteases, cytokines and collagens in hamsters infected with opisthorchis viverrini with special reference to peribiliary fibrosis and liver injury. | the liver fluke opisthorchis viverrini is endemic in southeastern asia, and causes cholangiocarcinoma and liver fibrosis. we investigated the time profile of the expression of matrix metalloproteinases (mmps) and tissue inhibitors of mmps (timps) in relation to peribiliary fibrosis in o. viverrini-infected hamsters. hepatic mrna expression of mmps, timps, cytokines and collagens i and iii was assessed by quantitative reverse transcription-pcr. zymography and immunohistochemistry were also used t ... | 2009 | 19168069 |
| intestinal parasitic infections in hill-tribe schoolchildren in chiang mai, northern thailand. | we surveyed intestinal parasitic infections in hill-tribe schoolchildren residing permanently in chiang mai province. the positive rate, of 403 stool specimens examined using the formalin-ether sedimentation technique, was 48.9%. no significant difference between male (50.8%) and female (47.1%) students was found for the infection. the most common protozoa was entamoeba coli (40.9%), followed by giardia lamblia (14.9%). the most common helminth was hookworm (13.4%), followed by ascaris lumbricoi ... | 2003 | 19230577 |
| studies on concomitant antigens of bithynia funiculata for detection of antibody to opisthorchis viverrini: effect of different centrifugal speeds on antigen preparation. | antigens derived from somatic extracts of bithynia funiculata, an intermediate snail host of o. viverrini, have been demonstrated to be highly heterogeneous in molecular weight (mw). these antigens have been suggested to be of potential use for serodiagnosis. in this study, b. funiculata somatic antigens were extracted using five different centrifugal speeds, namely 10,000 (c1); 20,000 (c2); 30,000 (c3); 40,000 (c4) and 50,000 (c5) rpm, with the aim of removing some non-specific antigens and det ... | 2003 | 19230581 |
| pcr diagnosis of opisthorchis viverrini and haplorchis taichui infections in a lao community in an area of endemicity and comparison of diagnostic methods for parasitological field surveys. | opisthorchiasis is a major public health problem in southeast asia. affected individuals often have mixed infections with the liver fluke opisthorchis viverrini and minute intestinal flukes such as haplorchis taichui. the usual methods of diagnosing these infections involve the demonstration of fluke eggs in stool samples under light microscopy, but sensitivity and specificity are low. we developed two pcr tests that detect and discriminate between o. viverrini and h. taichui infections. pcr tes ... | 2009 | 19279176 |
| cathepsin f cysteine protease of the human liver fluke, opisthorchis viverrini. | the liver fluke opisthorchis viverrini is classified as a class i carcinogen due to the association between cholangiocarcinoma and chronic o. viverrini infection. during its feeding activity within the bile duct, the parasite secretes several cathepsin f cysteine proteases that may induce or contribute to the pathologies associated with hepatobiliary abnormalities. | 2009 | 19308250 |
| dose-response relationships and tegumental surface alterations in opisthorchis viverrini following treatment with mefloquine in vivo and in vitro. | the treatment and control of opisthorchiasis relies on a single drug, praziquantel; hence, there is a need to develop novel opisthorchicidal drugs. we investigated the in vitro and in vivo activity of the antimalarial mefloquine against opisthorchis viverrini. hamsters infected with o. viverrini for 2 weeks (juvenile infections) and 4 weeks (adult infections) were treated orally with single 200-400-mg/kg oral mefloquine. worm burden reductions were assessed against untreated control hamsters. wo ... | 2009 | 19308455 |
| effect of praziquantel treatment on the expression of matrix metalloproteinases in relation to tissue resorption during fibrosis in hamsters with acute and chronic opisthorchis viverrini infection. | praziquantel has been used for the treatment of diseases caused by infection with various parasites. praziquantel treatment in opisthorchis viverrini-infected patients reduces the hepatobiliary fibrosis due to tissue resorption, however, some fibroses are irreversible. to clarify the effect of praziquantel treatment on hepatobiliary fibrosis, we examined the expression of matrix metalloproteinases (mmps) in relation to fibrolysis on praziquantel-treated hamsters after o. viverrini acute infectio ... | 2009 | 19427296 |
| [experimental study progress on tribendimidine, artemether and artesunate against clonorchis sinensis and other trematodes]. | currently praziquantel is one of the major drugs used in treatment of schistosomiasis and other trematode infections. recent experimental studies indicate that a new anthelmintic, tribendimidine, is used in the treatment of intestinal nematodes, also possesses effect against several species of trematodes including clonorchis sinensis, opisthorchis viverrini and echinostoma caproni. tribendimidine is even more effective against c. sinensis in rats that a single 300 mg/kg oral dose cures almost al ... | 2009 | 19459505 |
| impact of temporal changes and host factors on the genetic structure of a population of opisthorchis viverrini sensu lato in khon kaen province (thailand). | the population genetics of 317 individual opisthorchis viverrini from khon kaen province thailand, from 4 different years and 4 cyprinid fish species was examined using multilocus enzyme electrophoresis of enolase (enol), phosphoglucomutase (pgm) and triose phosphate isomerase (tpi). allele and genotype frequencies for enol and pgm were consistent irrespective of year or host species. no heterozygote deficiency was detected for enol. significant heterozygote deficiencies were detected in 3 of 4 ... | 2009 | 19523258 |
| discovery of opisthorchis viverrini metacercariae in freshwater fish in southern cambodia. | small liver flukes, clonorchis sinensis and opisthorchis viverini, are fish-borne trematodes (fbts) causing significant public health problems in asia. while c. sinensis is distributing mainly in far east asia, o. viverini is distributing in indochina peninsula. recently, however, the geographical distributions of those small liver flukes were proven to be far wider than expected. nevertheless, little is known about the epidemiology of small liver flukes in cambodia. the present study is, theref ... | 2009 | 19524077 |
| risk factors for cholangiocarcinoma in khon kaen, thailand: a nested case-control study. | the present nested case-control study within the khon kaen cohort study was conducted to assess risk factors for cholangiocarcinoma (cca) development. cases were 108 subjects with proven cca, by ultrasound at least, and controls also numbered 108, matched by sex, age (not more than 3 years difference) and period of recruitment to the cohort (not more than 3 months difference). a questionnaire was constructed based on that employed for the khon kaen cohort study recruitment. mcnemar's chi-square ... | 2009 | 19537893 |
| cholangiocarcinoma in experimental hamsters with long-standing opisthorchis viverrini infection. | liver fluke infection of opisthorchis viverrini (o. viverrini) is closely associated with several hepatobiliary diseases including cholangiocarcinoma (cca), but no reports have described these diseases in chronic and long-standing experimental opisthorchiasis in hamsters more than 10 months of age. a longer period of infection could induce different pathological lesions. to prove the hypothesis, we therefore sequentially investigated histological changes of the hepatobiliary system in 4 groups o ... | 2009 | 19537899 |
| incidence and risk factors of opisthorchis viverrini infections in a rural community in thailand. | opisthorchis viverrini infection is one of the major public health problems in thailand. although information about transmission of this parasite to humans is well established, infections are still prevalent in the northern and northeastern thailand. thus, prospective epidemiologic information is needed for developing more effective public health interventions. a cohort study to identify incidence and risk factors of o. viverrini infection was conducted in a rural community in thailand during de ... | 2009 | 19556581 |
| alterations of gene expression of rb pathway in opisthorchis viverrini infection-induced cholangiocarcinoma. | opisthorchiasis has the significant relationship with the high prevalence of cholangiocarcinoma (cca; a bile duct cancer) in the endemic areas in southeast asia. to reveal the molecular mechanism of the tumorigenesis induced by opisthorchis viverrini infection, the present study investigated the kinetic expression of rb pathway genes, including rb1, p16(ink4), cyclin d1, and cdk4, during the development of opisthorchiasis-associated cca in hamster model. the results of quantitative real-time pol ... | 2009 | 19582476 |
| food-borne trematodiases. | an estimated 750 million people are at risk of infections with food-borne trematodes, which comprise liver flukes (clonorchis sinensis, fasciola gigantica, fasciola hepatica, opisthorchis felineus, and opisthorchis viverrini), lung flukes (paragonimus spp.), and intestinal flukes (e.g., echinostoma spp., fasciolopsis buski, and the heterophyids). food-borne trematodiases pose a significant public health and economic problem, yet these diseases are often neglected. in this review, we summarize th ... | 2009 | 19597009 |
| turmeric reduces inflammatory cells in hamster opisthorchiasis. | the curcumin compound from turmeric is effective in the treatment of many inflammatory diseases. the aim of our present study was to evaluate the efficacy of turmeric on reducing the histopathological changes of hamster opisthorchiasis. hamsters were infected with opisthorchis viverrini and then administered turmeric. using light microscopic observation, liver function tests for alanine transaminase (alt), alkaline phosphatase, and direct bilirubin were investigated. the resulting histopathologi ... | 2009 | 19633869 |
| biological variation within opisthorchis viverrini sensu lato in thailand and lao pdr. | previous molecular genetic analyses indicate that opisthorchis viverrini is a complex of at least 2 cryptic species in thailand and the lao people's democratic republic (pdr). opisthorchis viverrini sensu lato (s.l.) populations can be divided into 6 genetic groups, which correlate with 5 different river wetlands systems. the present study examines the infectivity, growth, fecundity, and body size of o. viverrini s.l. isolates from these systems. worm recovery was lowest in the songkhram river, ... | 2009 | 19658451 |
| advanced periductal fibrosis from infection with the carcinogenic human liver fluke opisthorchis viverrini correlates with elevated levels of interleukin-6. | more than 750 million people are at risk of infection with foodborne liver flukes. opisthorchis viverrini is considered among the most important of these parasites, due to its strong association with cholangiocarcinoma (cca). o. viverrini infection results in a chronic inflammatory challenge to the host, which can lead to advanced, pathogen-specific disease sequelae including obstructive jaundice, hepatomegaly, cholecystitis, as well as cca. however, before disease sequelae are apparent, importa ... | 2009 | 19676135 |
| fasciola gigantica: immunodiagnosis of fasciolosis by detection of circulating 28.5 kda tegumental antigen. | a monoclonal antibody (moab)-based sandwich elisa was developed for the detection of circulating 28.5 kda tegumental antigen (28.5 kda ta) in the sera from mice experimentally infected with fasciola gigantica. the moab was immobilized on a microtiter plate, and the antigen in the serum was captured and detected with biotinylated polyclonal rabbit anti ta antibody. the test could detect 28.5 kda in the extracts of tegument (ta), whole body (wb) and excretory-secretory (es) fractions at the concen ... | 2009 | 19723524 |
| high prevalence of haplorchis taichui, phaneropsolus molenkampi, and other helminth infections among people in khammouane province, lao pdr. | the prevalence of liver and intestinal helminth infections, including opisthorchis, haplorchis, phaneropsolus, hookworms, enterobius, and taenia, was surveyed in khammouane province, lao pdr. fecal specimens were collected from 1,242 people (590 men and 652 women) in 3 mekong riverside villages and were examined by the kato-katz thick smear technique. the overall helminth egg positive rate was 81.1%. the positive rate for small trematode eggs, including opisthorchis viverrini, heterophyids, and ... | 2009 | 19724697 |