| schistosomiasis associated with rupture of the appendix in pregnancy. | acute appendicitis is among the most common indications for exploratory laparotomy during pregnancy. although usually pyogenic in origin, parasitic infections account for a small percentage of cases. we report here the association of pregnancy and appendicitis caused by schistosoma japonicum. schistosomiasis is a very common complication of pregnancy, with 250,000,000 persons infected worldwide, including 20% of pregnant women living in hyperendemic areas. schistosome egg masses can lodge throug ... | 1989 | 2503794 |
| mammographic microcalcifications associated with schistosomiasis. | we have described the first known occurrence of ectopic schistosoma japonicum of the breast mimicking the mammographic pattern of carcinoma. | 1989 | 2503893 |
| [dermal responses of various hosts to infection with schistosoma japonicum cercariae]. | the present investigation concerns with the dermal reactions produced in various host species by the invading schistosomula in primary infection. eight animal species including mouse, rat, hamster, jird, guinea pig, rabbit, rhesus monkey and pigeon were used. a small area of abdominal skin exposed to 300 schistosoma japonicum cercariae was taken at different time intervals from each animal species. | 1989 | 2504510 |
| a comparison of the antischistosomal effect of levo- and dextro-praziquantel on schistosoma japonicum and s. mansoni in mice. | praziquantel (pzq) is a racemic compound composed of equal proportions of its optical isomers, levo- and dextro-pzq. the efficacy of these compounds was compared with that of pzq in mice infected with schistosoma japonicum or s. mansoni. mice were given 50, 2 x 50 (on consecutive days), 500, or 2 x 250 mg/kg of each compound orally 5 weeks after infection. significant reduction of worm recovery was observed in s. japonicum infection 30 days after treatment with 2 x 50, 500, or 2 x 250 mg/kg of l ... | 1989 | 2505624 |
| schistosoma japonicum and s. mansoni: ultrastructural damage in the tegument and reproductive organs after treatment with levo- and dextro-praziquantel. | ultrastructural observations were made of changes in the tegument and reproductive organs of schistosoma japonicum and s. mansoni from icr mice after treatment with praziquantel (pzq), levo-pzq, and dextro-pzq at a single oral dose of 500 mg/kg body weight. no marked difference in types and extent of lesions of the tegument of s. japonicum was found between the compounds regardless of the time of worm recovery after treatment. this was equally true of s. mansoni. degeneration of the testis, ovar ... | 1989 | 2505625 |
| disappearance of specific antibodies in patients with chronic schistosomiasis japonica by treatment with praziquantel. | we tested effects of praziquantel, an antischistosomal compound, on clinical and immunological parameters of chronic schistosomiasis japonica. two japanese patients, who had high antibody titers to schistosoma japonicum antigens but no fecal schistosome eggs or no or mild symptoms complained, were treated with praziquantel. within two years after treatment, anti-schistosome antibodies in sera from the patients became negative in enzyme-linked immunosorbent assay. there was no significant alterat ... | 1989 | 2506387 |
| [further study on the immunity induced by mutagenic ntg attenuated cercariae of schistosoma japonicum]. | in order to understand the immunity induced by ntg attenuated cercariae of s. japonicum, investigations were carried out on the survival rate of mice, worm reduction rate, liver and spleen granulation etc. at different intervals between immunization and challenge (4, 6, 8, 10 and 12 wk) in mice exposed percutaneously to ntg attenuated cercariae (30 micrograms/ml ntg for 15 min). scanning electron microscopy on tegumental surface of schistosomes from immunized mice was also performed. results sho ... | 1989 | 2509101 |
| reduced levels of mutagen processing potential in the schistosoma japonicum-infected mouse liver. | epidemiological studies have shown the presence of a positive correlation between the infection of schistosoma japonicum and colorectal and/or liver cancers in the humans. to explore the mechanism underlying this correlation, we have investigated the mutagen-activating potentials of the liver homogenate fraction (s9) from schistosoma japonicum infected mice and those from control mice, by use of the ames test with 2-acetylaminofluorene, aflatoxin b1 and 3-amino-1-methyl-5h-pyrido[4,3-b]indole (t ... | 1989 | 2509904 |
| [a case of metastatic lung tumor of the colon cancer with ova of schistosoma japonicum in the resected lung specimen]. | a 69-year-old man underwent right middle lobectomy because of the tumor lesion in the right middle lung. he had ever been in celebes and china (riverside area of the yangtze), the infected area of schistosomiasis japonica, for military service during the world war ii. resected lung specimen was carefully examined at the department of pathology in our hospital. several ova of schistosoma japonicum were discovered scattered in the specimen, and the pathological feature of the lung lesion was revea ... | 1989 | 2512405 |
| schistosomiasis on bohol island, philippines, with special emphasis on the successful discovery of new habitats of the vector snail, oncomelania quadrasi, and area-wide mollusciciding. | in the endemic area of schistosomiasis on bohol island, we succeeded in locating seven new colonies of the vector snail, oncomelania quadrasi, four in trinidad and three in talibon, in addition to six locations from which the snails were previously recorded. we were led by the proximity of the snail habitat to the residence of the infected individuals. in northern bohol the continuous wet season would appear to make the major differences between the places that do and those that do not support o ... | 1989 | 2513438 |
| cross protection between a laboratory passaged chinese strain of schistosoma japonicum and field isolates of s. japonicum from china. | our laboratory strain of schistosoma japonicum has been isolated for 51 years, but is comparable to the indigenous chinese parasite in terms of its infectivity to both the intermediate and definitive hosts. vaccination with our strain protects mice against challenge with wild isolates of s. japonicum from china. thus any defined antigen vaccine developed using our laboratory strain would be expected to protect against s. japonicum in the field in china. | 1989 | 2513672 |
| [relationship between the effects of praziquantel on mice and rabbits with different intensities of schistosoma japonicum infection and humoral immunity levels of the host]. | rabbits were infected with 48-52, 198-202 and 498-502 cercariae of schistosoma japonicum and treated ig with single doses of praziquantel (40 mg/kg) 4 and 8 wk post infection. the worm reduction rates of the 8 wk groups were 87.9, 92.2 and 97%, respectively. these values were significantly higher than 48.5, 52.3 and 58.6% for the corresponding 4 wk groups. the serum antibody titres of the 8 wk post infection rabbits were apparently higher than those of the 4 wk rabbits. meanwhile, for the same d ... | 1989 | 2515748 |
| [a preliminary study on diagnosis of schistosomiasis japonica by indirect immunoperoxidase assay using daughter sporocyst-containing oncomelania tissue sections]. | schistosoma japonicum daughter sporocysts in oncomelania tissue sections were, for the first time, used as antigen in the diagnosis of schistosomiasis japonica by indirect immunoperoxidase assay (iipa-ds). the most strong peroxidase reaction was localized on the tegument of the daughter sporocysts and cercariae, and at some parts of cercarial parenchyma, when iipa-ds was carried out. of 112 sera from proven cases of schistosomiasis japonica, 106 (94.6%) were positive, the range of titre dilution ... | 1989 | 2517413 |
| [cellular responses elicited by challenged schistosomula of schistosoma japonicum in the skin of naive and chronically infected mice]. | naive and chronically infected c57bl/6 mice were challenged percutaneously over the ear pinna with schistosoma japonicum cercariae. after 15 hours, the number of eos increased significantly in the skin of chronically infected mice. inflammatory cells aggregated in the vicinity of schistosomula or entrapped intact and disintegrated schistosomula, forming granulocytic micro-abscesses in both groups. ultrastructure studies revealed that flattened eos tightly attached to the schistosomulum surface a ... | 1989 | 2517414 |
| [comparison of immunoenzymatic staining technique (iest), double gluing strip circumoval precipitin test (dgs-copt) and conventional circumoval precipitin test (cv-copt) for diagnosis of schistosomiasis japonica]. | iest, dgs-copt and cv-copt using lyophilized ova of schistosoma japonicum were performed on sera from 120 cases of schistosomiasis japonica, 120 cases of schistosomiasis japonica 3-8 years after being cured with praziquantel and 120 healthy individuals by single-blind method. the sensitivity and specificity of iest was 91.7% and 95.8% respectively which were significantly higher than that of both dgs-copt and cv-copt. the negative conversion rate of cured patients was 70.8% with iest, 80.8% with ... | 1989 | 2517415 |
| [humoral and cellular immunity in mice immunized with mutagenic ntg-attenuated cercariae of schistosoma japonicum]. | observation was made on humoral and cellular immunity in mice immunized with ntg-attenuated cercariae. results showed that the specific antibody in serum of immunized mice increased gradually. the titer by elisa and ifa were more than 1:1,280 respectively at week 7 post-immunization with ntg-attenuated cercariae (15 min. pc or 60 min. ip). sds-page electrophoresis showed that there was an extra-band of igg with mw, between 50,000 and 40,000 in the serum of immunized mice and there was no obvious ... | 1989 | 2517416 |
| [studies on antibody-dependent cell-mediated cytotoxicity to schistosoma japonicum schistosomula in mice]. | in the presence of antischistosomular serum (ab), the adherence of macrophages (mø) eosinophils (eos) and neutrophils(neu) to and the killing effect on schistosomula of schistosoma japonicum were studied with a mouse model. in vitro experiment showed that all the three kinds of effector cells could adhere to the surface of the schistosomula when opsonized with specific ab resulting in the significant increase in the percentage of dead schistosomula. when spa was added to the system, the percenta ... | 1989 | 2517417 |
| deficiency of interleukin-2 production upon addition of soluble egg antigen to cultures of isolated hepatic granulomas or hepatic granuloma cells from mice infected with schistosoma japonicum. | schistosoma japonicum-infected c57bl/6 mice show similar dynamics of hepatic granulomatous inflammation and delayed hypersensitivity elicited by soluble egg antigens (sea) which reach peak levels at 9 weeks of infection and then spontaneously regress. in an attempt to link the level of interleukin 2 (il-2) production to the spontaneous regression of hepatic granulomatous inflammation, the study determined the dynamics of il-2 production by sea-challenged isolated hepatic granulomas (hg) and cell ... | 1989 | 2526099 |
| identification of a multispecific lipoprotein receptor in adult schistosoma japonicum by ligand blotting analyses. | a 43-kda putative lipoprotein receptor (sj43) of adult schistosoma japonicum worms has been identified using ligand blotting techniques. single and two dimensional electrophoretic analyses showed that sj43 consisted of a single acidic polypeptide with multiple lipoprotein specificity. the molecule bound 125i-labelled low-density (apo-b), very low-density or high-density (apo-a and/or apo-c) lipoproteins from different mammalian hosts that are permissive to s. japonicum infection, but did not bin ... | 1989 | 2548094 |
| evaluation of the mutagenicity and the tumor-promoting activity of parasite extracts: schistosoma japonicum and clonorchis sinensis. | in relation to the observed association of carcinogenesis with parasitic infections, the mutagenicity of extracts of schistosoma japonicum and clonorchis sinensis was examined. in the bacterial mutagenicity tests using the ames salmonella typhimurium strains ta98, ta100, ta97 and ta102, and escherichia coli wp2 and wp2 uvra pkm101 schistosoma soluble egg antigen and a homogenate of adult schistosoma worms showed no positive responses either in the presence or in the absence of s9 mix. likewise, ... | 1989 | 2552310 |
| ultrastructural observations on damage to schistosomula of schistosoma japonicum by mouse neutrophils in vitro. | ultrastructure of schistosomula of schistosoma japonicum was studied in relation to the effect of mouse neutrophils in vitro. in the presence of antibody and complement, damage to the schistosomular tegument covered with many neutrophils began to appear within 1 h incubation. exfoliation of the granular cytoplasm of the tegument, disorganization of the muscle layers and vacuolation in the inner tissues were seen in a part of neutrophil-attached parasites by 2 h incubation. however, the majority ... | 1989 | 2559219 |
| effects of induction of anti-embryonation immunity on liver granulomas, spleen weight and portal pressure in mice infected with schistosoma japonicum. | balb/c mice sensitized with injections of viable immature schistosoma japonicum eggs had significantly fewer and smaller granulomas in the liver, lower portal pressure and smaller spleens at d + 75 of infection compared to similarly infected unsensitized controls. the portal pressure and spleen weights of the mice sensitized with immature eggs were not different from uninfected unsensitized mice of similar ages at d + 75 of infection. the results strongly support our hypothesis that it should be ... | 1989 | 2565075 |
| assessment of the prevalence and titer of antibodies to a candidate schistosomiasis vaccine molecule, sj26, in several human serum banks. | using immunoassay and immunoblotting approaches, antibodies to sj26, a glutathione s-transferase molecule (mr = 26,000) of schistosoma japonicum worms that is a vaccine candidate, have been sought in three large human serum banks. for these studies, a near-native recombinant sj26 molecule produced in escherichia coli was used, generally in elisas. anti-sj26 activity was detected readily in a high proportion of the sera at titres below 1:400 and appeared to be largely protein a-binding igg antibo ... | 1989 | 2571250 |
| comparison of four schistosome excretory-secretory antigens: phenol sulfuric test active peak, cathodic circulating antigen, gut-associated proteoglycan, and circulating anodic antigen. | several carbohydrate-containing antigens of schistosomes have been characterized and tests for these antigens or for the corresponding antibodies are increasingly being used for the diagnosis and clinical analysis of human schistosomiasis. phenol sulfuric active peak (psap) and cathodic circulating antigen (cca) are 2 soluble glycoproteins found in adult worms of schistosoma mansoni. these antigens have some similar characteristics including solubility in trichloroacetic acid and non-binding or ... | 1985 | 2580451 |
| granulomatous response in selective ige-deficient sja/9 mice infected with schistosoma japonicum. | granulomatous response was examined in selective ige-deficient sja/9 mice infected with schistosoma japonicum. the average size of granulomas formed around newly deposited eggs in sja/9 mice was significantly smaller than that in control c57bl/6 or sjl/j mice, although the numbers of adult worms recovered from each strain were comparable among them. thus, ige antibody specific to allergenic components of s. japonicum eggs seems to act as an amplifier for the formation of granulomatous lesions. | 1989 | 2592120 |
| induction of resistance in oncomelania hupensis nosophora against schistosoma japonicum, but not against paragonimus ohirai, using irradiated miracidia. | oncomelania hupensis nosophora snails sensitized with x-irradiated schistosoma japonicum miracidia demonstrated resistance against a following challenge infection with non-irradiated homologous miracidia. the resistance in o. h. nosophora against s. japonicum was acquired within 1 day of sensitization, and it was strongest in a group challenged at an interval of 3 days. the resistance persisted for at least 4 weeks. histological examinations revealed amoebocyte accumulation around the challenged ... | 1989 | 2592137 |
| [synthesis of acylates of niridazole and its analogs as schistosomicides]. | in order to decrease the toxicity and enhance the curative effect of niridazole against schistosomiasis japonica, a series of acylates of niridazole has been prepared through acylation of niridazole, 2-substituted acetamido-5-nitrothiazoles and 1-(5-nitro-2-thiazolyl)-4-acylpiperazine were also prepared. the products has been tested against schistosomiasis japonica in mice. preliminary test results showed that the majority of aliphatic acylates of niridazole exhibited marked schistosomicidal eff ... | 1989 | 2609988 |
| histochemical evidence of the catecholamine-associated nervous system in certain schistosome cercariae. | the localization of catecholamines was documented in the cercaria of schistosoma mansoni and s. japonicum by a fluorescent histochemical method using glyoxylic acid (ga). cell bodies and nerve fibres were spatially visualized in whole-mount preparations, and the fluorescent traces were investigated. the nervous system was bilaterally symmetrical, showing a similar formation in both species. a pair of cerebral ganglia with a transverse commissure showed a complex network of fluorophores, and each ... | 1989 | 2616566 |
| glucose metabolism of adult schistosoma japonicum as revealed by nuclear magnetic resonance spectroscopy with d-[13c6]glucose. | excretory end-products of adult schistosoma japonicum, fed d-[13c6]glucose in vitro under aerobic and anaerobic conditions, were studied using 1h- and 13c-nuclear magnetic resonance (nmr) spectroscopy. the glucose in the medium is degraded to produce lactate and alanine aerobically and succinate and acetate as well as lactate and alanine anaerobically. succinate and acetate have not been previously recorded as excretory products resulting from the metabolism of glucose for schistosomes. the pres ... | 1989 | 2616648 |
| schistosoma japonicum: the effect of serum fractions precipitated by ammonium sulfate on viability of schistosomula. | the effect of serum fractions precipitated by a saturated ammonium sulfate solution on viability of schistosoma japonicum schistosomula was investigated. axenized cercariae were incubated for 10 days at 37 degrees c in nctc 109 supplemented with the 34%, 40%, 50%, 62% and 68% fractions of human or rabbit serum in an equivalent concentration to 10% serum. only larvae cultured in media with the 68% fraction of human or rabbit serum retained the transparent feature and active motility, while the la ... | 1989 | 2622065 |
| human chorionic gonadotropin-like immunoreactivity in schistosomes and fasciola. | | 1989 | 2622900 |
| vaccination against ovine cysticercosis using a defined recombinant antigen. | cysticercosis caused by larval tapeworms is a major public health problem and a cause of substantial economic losses in the farm-animal industries. taenia ovis in sheep is a particularly important example. immunity to reinfection with the larvae has a central role in regulating natural transmission of the parasites, and vaccination with antigens from the early larval oncosphere stage can induce complete protection against infection. as it is impractical to obtain enough oncospheres for a commerc ... | 1989 | 2648160 |
| characterization of a large gene family in schistosoma japonicum that encodes an immunogenic miracidial antigen. | three of eleven clones isolated from a genomic expression library of schistosoma japonicum dna using chronically infected human sera also react with chronically infected mouse sera. characterization of these three clones showed that they contain different members of the same gene family. one clone contains two members of the gene family approximately 2 kb apart and in opposite orientation to each other. dna sequence homologies between pairs of genes range from 98% to 99.5%. southern hybridizatio ... | 1989 | 2657419 |
| soluble proteins from schistosoma mansoni and japonicum: a comparative biochemical and immunological analysis. | 1. soluble proteins were recovered from male schistosoma mansoni after homogenization in tris-hcl buffer containing 0.6 m kcl and 1.0% triton x-100 followed by preparative electrophoresis on sds-gel. 2. polyclonal antibodies produced in mice against the soluble fraction were used in comparative analysis of s. mansoni and s. japonicum using immunoblots and immunoprecipitation of in vitro translated polypeptides. 3. small molecular weight polypeptide (20-22 kdalton), identified by infected mouse s ... | 1989 | 2667879 |
| [advances in research on the culturing of schistosoma japonicum in vitro]. | | 1989 | 2670314 |
| [production and characterization of monoclonal antibodies to echinococcus multilocularis]. | spleen cells from balb/c mice immunized against echinococcus multilocularis were fused with mouse myeloma cells (p3x6.5.3.) in the presence of polyethylene glycol (peg 1000), and the hybridomas were obtained. of the 85 hybridomas, 23 were found to produce antibodies. of the 23 cell lines, 13 hybridomas which produced a high titer of antibodies were cultured for cloning the cells by the limiting dilution method. then 5 monoclonal antibodies were obtained. the immunoglobulin class of the monoclona ... | 1989 | 2684834 |
| comparison of morphology, pathogenicity and drug response among three isolates of schistosoma japonicum in the mainland of china. | geographic differences among isolates of schistosoma japonicum from anhui province (a) in the east, guangxi autonomous region (g) in the south and yunnan province (y) in the southwest of mainland of china were studied. c57bl mice were used to compare these isolates. morphologically, both male and female worms of isolate y were shorter than those of isolates a and g, and the ovary of female worm of isolate g was the smallest. in isolate a, the percentage of male worms bearing 7 testes was 87%, wh ... | 1989 | 2735700 |
| screening for molluscicidal activity in crude drugs. | thirty-four extracts of crude drugs and medicinal plants have been screened for activity against oncomelania nosophora, the intermediate host of the japanese strain of schistosoma japonicum. strong molluscicidal activity was found in the meoh extract of anemarrhenae rhizoma. although timosaponin a-iii, one of the main saponins of anemarrhenae rhizoma, showed very strong killing activity, markogenin-3-o-beta-d-glucopyranosyl (1-2)-o-beta-d-galactopyranoside having the same glycosidic linkage was ... | 1989 | 2766410 |
| carbohydrate metabolism in adult schistosomes of different strains and species. | in this comparative study the carbohydrate metabolism of schistosoma japonicum, s. haematobium, s. intercalatum, s. bovis and three strains of s. mansoni was investigated. no large differences were found in the protein and glycogen contents of all species involved. in all species investigated, lactate was the main end product of carbohydrate breakdown. however, all parasites degraded part of the glucose to co2 via the krebs cycle. no difference was observed in the contribution of this aerobic pr ... | 1989 | 2777465 |
| [a study on mechanism of skin penetration by cercaria of s. japonicum and screening of preventive drugs]. | mice pretreated with pg cyclo-oxygenase inhibitor aspirin, at 300 mg/kg/d x 5, and ibuprofen, at 100 mg/kg/d x 5 orally and 20 mg/kg/d x 3 intraperitoneally revealed moderate chemoprophylactic effects and the reduction of the penetration of worms by 23% when compared to controls. daily oral administration of aspirin, at 300 mg/kg for 15 days the worms reduction increased to 41.7%. in estimation of pge1 in cercariae of s. japonicum by radioimmunoassay, the pge1 was 49 pg/100 cercariae. in normal ... | 1989 | 2793135 |
| integration and expression of murine retrovirus-related sequences in schistosomes. | antibodies against the retrovirus envelope glycoprotein (gp70) of mouse xenotropic retrovirus, balb virus 2 (bv2) reacted with the adult worms of schistosoma japonicum and s. mansoni. this reaction was completely inhibited after adsorption of the antibodies with virions of retrovirus. the reactive schistosome antigen was located in the subtegumental layer of the adult male fluke and in the vitelline gland of the adult female of s. japonicum and s. mansoni. proteins extracted from both parasites ... | 1989 | 2797870 |
| schistosoma mansoni, s. japonicum, and s. haematobium: permeability to acidic amino acids and effect of separated and unseparated adults. | permeability of the tegument of male and female schistosoma mansoni was measured in vitro and a comparison was made between copulating and separated worms. in unpaired (separated) schistosomes, a carrier-mediated (selective) transport system for acidic amino acids was demonstrated. males and females exhibited similar uptake rates for aspartate and glutamate. half-saturation constants for aspartate (males, 0.035 +/- 0.008 mm; females, 0.026 +/- 0.006 mm) and glutamate (males, 0.010 +/- 0.007 mm; ... | 1985 | 2860003 |
| interaction of schistosoma japonicum with salmonellae and other gram-negative bacteria. | the in vitro and in vivo interaction of schistosoma japonicum with salmonellae and other gram-negative bacteria was studied. in vitro, s. japonicum associated with salmonellae and other gram-negative bacteria, and more male than female schistosomes associated with the bacteria. by using the various strains and mutants of salmonellae, we showed that salmonella typhimurium had a higher degree of association than did salmonella enteritidis and that the piliated strains of s. typhimurium associated ... | 1985 | 2864378 |
| concomitant cellular and humoral expression of a regulatory cross-reactive idiotype in acute schistosoma japonicum infection. | in this study the expression of a regulatory cross-reactive idiotype (sj-crim), which is associated with anti-soluble egg antigen (sea) molecules in murine schistosoma japonicum infection, is described. both humoral and cellular components of the immune response were analyzed during the course of infection with s. japonicum. in the humoral immune response, the content of sj-crim decreases as the titer of anti-sea antibody increases throughout infection. quantitatively, values for serum ranged fr ... | 1986 | 2873105 |
| praziquantel in treatment of cerebral schistosomiasis. | the effectiveness of standard single-day praziquantel treatment (60 mg/kg) was prospectively evaluated in nine patients with seizures caused by cerebral schistosoma japonicum infection. eight patients were cured when discharged from the study, an average of 6 months after receiving praziquantel. they were seizure-free in the absence of anticonvulsants, their electroencephalograms were normal, and no major abnormalities could be detected on neurological examination. serial computed tomography sca ... | 1986 | 2875279 |
| [inhibition of tegumental gamma-glutamyltranspeptidase of schistosoma japonicum by praziquantel]. | | 1987 | 2887303 |
| vaccination against immunopathological disease in schistosomiasis japonica. | | 1985 | 2934072 |
| adult schistosome cdna libraries as a source of antigens for the study of experimental and human schistosomiasis. | protective immunity has been demonstrated in experimental schistosomiasis and is also believed to occur in man. it can be mediated by antibodies from infected animals or animals immunized with attenuated organisms. recombinant escherichia coli synthesizing antigenic polypeptides from the three principal species of schistosome that infect man, schistosoma mansoni, s. japonicum and s. haematobium, have been constructed. libraries of adult worm cdna were prepared from each species in the expression ... | 1986 | 2938004 |
| molecular cloning of schistosome genes. | | 1986 | 2940502 |
| isoenzyme analyses of malaysian schistosoma, s. mekongi and s. japonicum by isoelectric focusing in polyacrylamide gel. | isoenzyme patterns of adult malaysian schistosoma, s. mekongi and s. japonicum strains were analysed by isoelectric focusing (ief) in polyacrylamide gel. enzyme patterns obtained from malaysian schistosoma homogenates differed from those of s. mekongi and s. japonicum strains. malaysian schistosoma was found to differ from s. japonicum by 8 enzymes, namely phosphoglucomutase, phosphoglucoisomerase, malate dehydrogenase, acid phosphatase, hydroxy-butyrate dehydrogenase, hexokinase and alkaline ph ... | 1985 | 2940688 |
| [prophylactic activity of praziquantel in animals infected with schistosoma japonicum]. | | 1985 | 2943124 |
| [histological observations on schistosomula of schistosoma japonicum in mouse lung and liver after treatment with praziquantel]. | | 1985 | 2945377 |
| [effect of praziquantel in vitro on different developmental stages of schistosoma japonicum]. | | 1986 | 2945394 |
| [attachment of mouse neutrophils in vitro on the praziquantel-damaged surface of adult schistosoma japonicum in the presence of immune sera]. | | 1986 | 2946158 |
| [immune-complex nephropathy in mice infected with schistosoma japonicum]. | | 1986 | 2948734 |
| [histological observations on the effects of artemether, fuvinazole and niridazole on schistosoma japonicum schistosomulae in the mouse liver]. | | 1986 | 2954379 |
| [morphological alterations of schistosomulae invading the skin of mice treated orally with praziquantel before infection and cellular reaction of the skin]. | | 1986 | 2954400 |
| [schistosomicides and acetylcholinesterase in the tegument of the male schistosoma japonicum]. | | 1986 | 2955651 |
| [effect of praziquantel on mice immunity in the early stage after infection with schistosoma japonicum cercariae]. | | 1987 | 2955661 |
| [susceptibility of schistosomula at various ages to praziquantel during invasion in mouse skin and observations on certain characteristics of the schistosomula surface]. | | 1987 | 2959025 |
| deficiency of interleukin-2 activity upon addition of soluble egg antigen to cultures of spleen cells from mice infected with schistosoma japonicum. | schistosoma japonicum-infected c57bl/6 mice show similar dynamics of hepatic granulomatous inflammation (hgi) and delayed hypersensitivity (dh) elicited by soluble egg antigens (sea) which reach peak levels at 9 weeks of infection and then spontaneously regress. the in vitro sea-induced proliferation of spleen cells (sc) from infected animals attained its high point and then declined when sc from 5-week-infected mice were used. the present study determined the dynamics of interleukin-2 (il-2) pr ... | 1988 | 2968321 |
| schistosoma mansoni and s. japonicum worm numbers in 129/j mice of two types and dominance of susceptibility in f1 hybrids. | in a study on the genetics of resistance to schistosomiasis in wehi 129/j mice, susceptibility to either schistosoma mansoni or schistosoma japonicum was shown to be unequivocally dominant in f1 hybrid crosses between genetically resistant wehi 129/j and susceptible balb/c mice. the operation of only 1 or 2 genes in the expression of resistance to s. mansoni was suggested by backcross analysis. thus, approximately 25% of (balb/c x wehi 129/j) f1 x wehi 129/j mice were resistant to s. mansoni inf ... | 1988 | 2969409 |
| dynamics of collagen accumulation and polymorphism in murine schistosoma japonicum. | the dynamics of hepatic collagen biosynthesis and degradation were studied in mice infected with schistosoma japonicum. hepatic fibrosis, the major clinical manifestation of disease, increased during acute infection (0-15 wk). the majority of proline incorporation into hydroxyproline, which was reflective of collagen synthesis, was found within hepatic egg granulomas. as the disease became chronic (20-30 wk) there was a decrease in collagen synthesis and a maintenance of total collagenolytic act ... | 1985 | 2991069 |
| iron, ferritin, hepatitis b surface and core antigens in the livers of chinese patients with hepatocellular carcinoma. | surgically resected specimens, consisting of tumor and adjacent non-neoplastic liver tissue, were obtained from 40 patients with primary liver cancer at zhong shan hospital, shanghai medical university, the people's republic of china, between march 1983 and july 1984. all were hepatocellular carcinomas (hcc), one being admixed with cholangiocarcinoma. the relationship of hepatitis b virus (hbv) markers with iron and ferritin was evaluated in liver tissues from patients with primary liver cancers ... | 1987 | 3028601 |
| isolation of two immunogenically different allergens from schistosoma japonicum eggs. | two allergenic components, termed j1 and j2, were isolated from a soluble egg antigen preparation (sea) of schistosoma japonicum by anion-exchange chromatography on de52 and gel chromatography on sephacryl s-200. the apparent molecular weights of j1 and j2 were 260,000 and 46,000, respectively, by gel chromatography on sephadex g-150. by sds-polyacrylamide gel electrophoresis, both j1 and j2 showed apparent homogenicity and their estimated molecular weights were 135,000 and 45,000, respectively. ... | 1987 | 3034785 |
| cloning of a schistosoma japonicum gene encoding a major immunogen recognized by hyperinfected rabbits. | a library of randomly sheared schistosoma japonicum genomic dna fragments was constructed in the bacteriophage expression vector lambda gt11. a portion of the library was screened with sera collected from rabbits 8 weeks after they were infected with 1000 cercariae. four clones whose recombinant gene products react with the rabbit sera were purified to homogeneity. clone sjir-12a was chosen for detailed study because of its very intense reaction with the rabbit sera. sjir-12a was found to encode ... | 1987 | 3041213 |
| single-step purification of polypeptides expressed in escherichia coli as fusions with glutathione s-transferase. | plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in escherichia coli as fusions with the c terminus of sj26, a 26-kda glutathione s-transferase (gst; ec 2.5.1.18) encoded by the parasitic helminth schistosoma japonicum. in the majority of cases, fusion proteins are soluble in aqueous solutions and can be purified from crude bacterial lysates under non-denaturing conditions by affinity chromatography on immobilised glutathione. using batch wash pr ... | 1988 | 3047011 |
| granulocyte-macrophage colony-stimulating factor produced by splenic t lymphocytes of mice infected with schistosoma japonicum. | the production of granulocyte-macrophage (gm) colony-stimulating factor (csf) by splenic lymphocytes was examined in murine schistosomiasis japonica. when splenic lymphocytes obtained at various weeks after infection were cultured with soluble egg antigen, gm-csf activity in the conditioned medium became detectable at 3 weeks after infection, reached a peak at week 5, and persisted at least up to week 7. not only soluble egg antigen but also concanavalin a was highly effective in stimulating spl ... | 1986 | 3079731 |
| low molecular weight eosinophil chemotactic factor (ecf) in the serum of murine schistosomiasis japonica. | eosinophil chemotactic activity was detected in the serum obtained at an acute stage of murine schistosomiasis japonica. gel filtration of the dialyzable fraction of the serum on sephadex g25 showed that the chemotactic component had an apparent molecular weight of less than 1,000. it was stable to heating, but was sensitive to pronase or carboxypeptidase a digestions, indicating its peptide nature. eosinophil chemotactic activity of the dialysate of the serum from mast cell-deficient w/wv mice ... | 1986 | 3080375 |
| the intensity of experimental schistosome infections modulates hepatic pathology. | groups of mice infected with schistosoma japonicum were killed 7, 10 or 15 weeks after infection. the size of hepatic granulomas and degree of hepatic fibrosis in individual mice were examined in relation to the number of worm pairs present using regression analysis. mice with heavy s. japonicum infections had smaller circumoval granulomas in their livers than did mice with lighter infections. hepatic fibrosis per unit infection, i.e., per worm pair or per egg, also decreased as the intensity of ... | 1986 | 3080915 |
| isoenzymes of phenol oxidase in adult female schistosoma japonicum. | isoelectric focusing was used to study the phenol oxidase isozymes in adult female worms of schistosoma japonicum. more than one form of phenol oxidase has been demonstrated in extracts of female worms when incubated with 3,4-dihydroxyphenylalanine (dopa), catechol or cresol as substrates. dopa is the best substrate among all of them. the 5-6 bands of phenol oxidase exhibit pi values in the range of 6.0-7.5, the major band is at pi 6.0. | 1986 | 3083253 |
| influence of salmonella and other gram-negative bacteria on the survival of mice infected with schistosoma japonicum. | | 1986 | 3086463 |
| [studies on the diagnosis of schistosomiasis japonica. (4). effects of serum dialysis and anthelmintic treatment of the patients on the determination of cop value with special reference to the black eggs]. | eggs of schistosoma japonicum were obtained from the livers and intestines of the mice infected subcutaneously 50 cercaria 7 weeks previously. a dialyzing celophane tube containing 1 ml of serum was immersed and stirred in 500 ml nacl solution with different molar concentrations and dialyzed for 24 hrs at 4c. thereafter, the sera of infected human and animals were incubated with an equal volume of egg-suspension (average 100 eggs per drop). the results indicated that a close relationship between ... | 1986 | 3087851 |
| the killing effect of subclasses of mouse igg antibodies on schistosomula of schistosoma japonicum. | the killing effect of igg antibodies in sera from s. japonicum infected mice on schistosomula of s. japonicum was studied in vitro in the presence of complement. immune mouse sera were fractionated over protein a-sepharose and igg1, igg2a, igg2b and igg3 were purified by affinity chromatography. the killing activity of each subclass of igg antibodies against schistosomula was observed only in the presence of complement. the activity of igg1 was higher than that of the other subclasses of igg. in ... | 1986 | 3088303 |
| the diagnosis of schistosoma japonicum infection. areas of uncertainty. | | 1986 | 3088782 |
| population studies on oncomelania quadrasi, the snail intermediate host of schistosoma japonicum, in the philippines. 5. quantitative analysis on successful snail control by land reclamation. | for the control of oncomelania quadrasi, environmental modifications, i.e., clearing of vegetation, leveling of swampy depression and draining of stagnant water by channeling and excavation were carried out at 3 areas in leyte, philippines from 1974 to 1977. the change of snail population resulted in the land reclamation was evaluated by the methods previously developed by the population studies on this snail. as a result of statistical analysis based on y = log(x + 0.01) transformation and the ... | 1986 | 3090317 |
| [clinical significance of chronic schistosomiasis japonica--histological detection of ova of schistosoma japonicum]. | | 1986 | 3090318 |
| an attempt to study the case fatality rate in schistosoma japonicum infection in the philippines. | as a sequence to a cross-sectional study on the clinical gradient of the disease by pesigan et al., (1965) a follow-up of the subjects in that study was made after 12 years. of the 135 untreated cases followed up, 23 (17.04%) died from various causes of which 12 (8.89%) had signs and symptoms attributable to schistosomiasis as the immediate cause or one of the main causes of death. this occurred in 1 to 11 years with an average of 5 years, which corresponds roughly to 1.78% of the infected cases ... | 1986 | 3090714 |
| the ability of single sex infections of schistosoma japonicum to induce resistance to reinfection in mice. | in four experiments, mice harbouring an average 50, 76 or over 200 schistosoma japonicum female worms were not resistant when challenged six to eight weeks after infection. the female worms from these single sex infections were stunted and immature (average length 4.6 mm) and induced no overt pathology in the host. male worm burdens of 60, 135 or 140 also induced little or no resistance to challenge in the host. the males from these single sex infections were fully grown for their age (average l ... | 1986 | 3091678 |
| stage and species specificity of antigens encoded by two geographic strains of schistosoma mansoni mrna. | proteins translated in vitro from schistosoma mansoni adult worm mrna were assessed for their antigenic specificities compared to different stages, strains and species of the parasite. rna was extracted from both puerto rican and brazilian parasites and directed the synthesis of high molecular weight proteins. preabsorption of immune human serum with schistosomula was used to determine whether the in vitro translated proteins contained antigens shared between the adult and this immature stage. t ... | 1986 | 3091803 |
| cardiopulmonary involvement rare in severe schistosoma japonicum infection. | in a prospective study, 65 consecutive patients hospitalized with schistosoma japonicum infection were evaluated for evidence of cardiopulmonary involvement. thirty-two patients had evidence of severe liver dysfunction while 11 patients had portal hypertension only. of these 43 patients with hepatosplenic disease, only one patient had pulmonary hypertension. cardiopulmonary involvement is relatively uncommon in severe s. japonicum infections. | 1986 | 3092416 |
| preliminary studies on shared antigens among cercaria, adult worm and egg of schistosoma japonicum. | | 1986 | 3093167 |
| [praziquantel and its levo-and dextro-isomers for the treatment of rabbits infected with schistosomiasis japonica]. | | 1986 | 3093175 |
| [application of self-made alkaline phosphatase for elisa in schistosomiasis japonica]. | | 1986 | 3094978 |
| [a field investigation on the probability of the dispersion of schistosoma japonicum eggs to oncomelania-infested bodies of water in 4 villages of wuxian county]. | | 1986 | 3094979 |
| dynamics of hepatic glycosaminoglycan accumulation in murine schistosoma japonicum infection. | hepatic fibrosis is the major pathologic sequela of schistosomiasis and is comprised of extracellular collagen and proteoglycan macromolecules. glycosaminoglycans (gags), the major constituents of proteoglycan molecules, were studied in mice infected with schistosoma japonicum. total gag content was measured by extraction of uronic acid from murine liver sections and isolated hepatic granulomas during the progression from acute (0-15 wk) to chronic (20-30 wk) infection. infected livers contained ... | 1986 | 3095167 |
| inhibition of immediate and arthus responses to schistosome egg antigens by t cells from schistosoma japonicum-infected mice. | schistosoma japonicum-infected mice develop hepatic granulomas, immediate hypersensitivity (ih), and delayed hypersensitivity (dh) to soluble egg antigens (sea) released by parasite eggs trapped in liver sinusoids. all of these responses spontaneously regress after 7 to 9 weeks of infection. this study aimed to develop an in vivo system for the further dissection of cellular and humoral immune responses to sea. c57bl/6 mice immunized subcutaneously with sea in complete freund adjuvant developed ... | 1986 | 3095244 |
| antibodies of patients infected with schistosoma japonicum crossreact with diagnostic proteins of schistosoma mansoni. | | 1986 | 3095559 |
| mr 26,000 antigen of schistosoma japonicum recognized by resistant wehi 129/j mice is a parasite glutathione s-transferase. | mice of the inbred strain 129/j bred at this institute (wehi 129/j) are relatively resistant to chronic infection with the parasitic helminth schistosoma japonicum. in contrast to more permissive mouse strains such as balb/c, the wehi 129/j mice are high responders to a mr 26,000 adult worm antigen designated sj26. cloned cdnas corresponding to sj26 have been identified in a s. japonicum phage lambda gt11 amp3 expression library, and their nucleotide sequences have been deduced. the predicted am ... | 1986 | 3095841 |
| [the sensitizing role of the gut-associated antigen of schistosoma japonicum in granuloma formation around the ova]. | | 1986 | 3096536 |
| eosinophil chemotactic lymphokine produced by egg-associated granulomas in murine schistosomiasis japonicum. | eosinophil chemotactic factor (ecf) was detected in the culture supernatant of isolated intact egg granulomas from the livers of schistosoma japonicum-infected mice. this factor had an apparent molecular weight of 15,000 by high-pressure liquid chromatography with an sw3000 column and bound to concanavalin a-sepharose 4b. when cells obtained by enzymatic digestion of isolated granulomas were cultured under the presence of soluble egg antigen of s. japonicum or concanavalin a, ecf was also detect ... | 1986 | 3096889 |
| immunoglobulins inside schistosoma japonicum eggs from the livers of mice. | using fluorescent antibody techniques, immunoglobulins (ig's), mainly igg class, were detected inside schistosoma japonicum eggs lodged in mouse liver. ig's were observed as a focal pattern between the miracidium and the eggshell during early infection (5-7 wk), particularly in lightly infected mice (20 cercariae). with advancement of time of infection (8-18 wk), a diffuse pattern of staining over the miracidial body developed and became predominant. the diffuse pattern of staining could be obse ... | 1986 | 3097294 |
| schistosoma japonicum-like infection in phichit province, northern thailand: a case report. | a case of human schistosomiasis from phichit province is presented. schistosome eggs were found in the ileo-caecal mass of a 44-year old woman, native of sak-lek, muang district. histologic pictures revealed an early acute granulomatous lesion which consisted of predominantly eosinophils without multinucleated giant cells and fibrotic change suggesting a recent infection. on the basis of the shape and microscopic appearance of the eggs, they are smaller than those described previously for schist ... | 1986 | 3097834 |
| [experimental observation on the latex agglutination test in the detection of anti-schistosome antibody]. | | 1986 | 3098446 |
| serum igg, igm, and c3 levels in schistosoma japonicum infected rabbits. | new zealand white rabbits were infected with 250 or 500 schistosoma japonicum cercariae of philippine-leyte strain. following the initial pre-infection bleed, blood was collected 2, 4, 6, 8, 11, 14, 17, 20, 23, 26, 29, and 32 weeks post-infection, and igg, igm, and c3 levels were assayed in the serum samples by single radial immunodiffusion. igg and igm achieved peak levels at 14 weeks post-infection and began to decrease in concentration by 26 weeks. at 32 weeks, some of the infected rabbits ex ... | 1986 | 3099128 |
| [effect of pyquiton on some histochemical activities of schistosomules of schistosoma japonicum in mice]. | | 1986 | 3100088 |
| [the application of biotin-avidin system in the diagnosis of schistosomiasis japonica]. | | 1986 | 3100091 |
| in vitro cultivation of schistosoma japonicum schistosomula. | | 1986 | 3100156 |
| efficacy of single oral doses of praziquantel in treatment of schistosoma japonicum infection. | | 1986 | 3100187 |
| in vitro cultivation of schistosoma japonicum from cercariae to egg-producing adult worms. | | 1986 | 3100229 |