| trypanosoma cruzi: immune destruction of parasitized mouse fibroblasts in vitro. | | 1977 | 402286 |
| human behaviour and the propagation of chagaś disease. | during 12 months, 26 households with 172 residents in são felipe, bahia, brazil, entertained 74 different guests who were potentially exposed to attack by panstrongylus megistus for an average of about 23 nights per guest per year. during the same period 52 of the residents spent an average of about two weeks a year away from home. these exchanges established contact with 79 other households in the district. these and other observations reinforce the opinion that human behaviour plays an importa ... | 1976 | 402723 |
| trypanosoma cruzi: infection of normal and activated mouse macrophages. | | 1977 | 403086 |
| [studies on wild reservoirs and vectors of trypanosoma cruzi. lxiii: natural infection of the marsupial caluromys lanatus ochropus (wagner, 1842) by t. cruzi]. | | 1977 | 403576 |
| [studies on wild vectors and reservoirs of trypanosoma cruzi. lxii: evolution of t. cruzi in the coelomic cavity of triatomines]. | | 1977 | 403578 |
| survival of arboviruses in trypanosome-infected triatomines. | the potential of triatomines to maintain arboviruses was demonstrated by the ability of rhodinius prolixus with experimentally punctured abdomen to harbor venezuelan equine encephalitis (vee) virus for at least 4 months and st. louis encephalitis virus for 1 month. at 30 days after infection vee virus was found at low titers in trypanosoma cruzi-infected r. prolixus and at moderate titers in t. rangeli-infected r. neglectus. transmission of vee virus by bite of punctured bugs was successful 2 we ... | 1977 | 403822 |
| cellular immunity in chronic chagas' disease. | the cellular immune response was assessed in 20 patients with chronic chagas' disease (american trypanosomiasis). thymus-derived lymphocyte function was determined in vivo by cutaneous reactivity to several antigens including a soluble preparation derived from trypanosoma cruzi and sensitization to 2,4-dinitrochlorobenzene. the in vitro t-cell reactivity was investigated by the proliferative response to phytohemagglutinin and to t. cruzi antigen and by inhibition of leukocyte migration with the ... | 1977 | 404317 |
| serological methods in the diagnosis of chagas' disease. | | 1977 | 404735 |
| effect of the injection of ribosomes and rna from crithidia fasciculata on the experimental infection of mice by trypanosoma cruzi. | immunization of mice with crithidia fasciculata (live suspension, ribosomal fraction and purified rna) induced a certain degree of protection (decrease of parasitaemia) against infection with trypanosoma cruzi. | 1977 | 404736 |
| immunization of mice against trypanosoma cruzi. the effect of size of dose, and route of injection, of immunizing and challenge inocula. | groups of cd-1 mice were immunized with a vaccine prepared from freeze-thawed cultured epimastigotes of trypanosoma cruzi, strain y, with or without saponin as adjuvant. the vaccine was injected by the subcutaneous (s.c.) or intraperitoneal (i.p.) route. immunized and control mice were challenged with homologous blood trypomastigotes by the s.c. or i.p. route and the former route was found to produce a far more severe and uniform infection in both control and immunized mice. subcutaneous challen ... | 1977 | 404742 |
| effect of length of treatment with bayer 2502 on isolation of trypanosoma cruzi and resistance to challenge in the mouse. | the effect of length of treatment of trypanosoma cruzi with bayer 2502 (bay 2502, lampit, nifurtimox) on resistance to challenge and parasite isolation was studied in two experiments. in each, 200 mice were divided into groups of 20, infected with t. cruzi, and then treated with bay 2502 for 1 to 8 consecutive weeks. sixteen weeks after exposure, 10 mice from each experimental group were challenged with 150,000 trypomastigotes. the remainder were maintaned for 8 more weeks and killed for isolati ... | 1977 | 405877 |
| antiprotozoal thiazoles. 2. 2-(5-nitro-2-furyl-, thiazolyl-, and 1-methylimidazolyl-)thiazoles. | ten 2-substituted 4-thiazolecarboxaldehyde hydrazones bearing 5-nitro-2-furyl, 5-nitro-2-thiazolyl, and 1-methyl-5-nitro-2-imidazolyl functions have been prepared and screened for activity against trypanosoma cruzi infections in mice. the results permitted the ranking of these substituents in decreasing order of activity: 1-methyl-5-nitro-2-imidazolyl greater than 5-nitro-2-furyl greater than 5-nitro-2-thiazolyl, the last being inactive. some structural features of the side chain necessary for o ... | 1977 | 406399 |
| [studies on wild disease reservoirs and vectors of trypanosoma cruzi: lxiv. natural infection of the rodent dasyprocta aguti (linnaeus, 1766) by t. cruzi]. | | 1977 | 406645 |
| [studies on the wild reservoirs and vectors of trypanosoma cruzi. lxv. natural infection of the monkey callithrix geoffroyi (humboldt, 1812) by t. cruzi]. | | 1977 | 406646 |
| naturally occurring trypanosomiasis (chagas' disease) in dogs. | nine fatal cases of canine american trypanosomiasis (chagas' disease) were encountered from november, 1972, through november, 1975. of the 9 cases, 7 occurred in dogs from 5 central texas counties, and all but one case was diagnosed during the months of september, october, or november. the source of infection was discovered in only one case--that being a doghouse heavily infested with triatoma lectularius occulta. each of 6 bugs collected had infective forms of the protozoan, trypanosoma cruzi. ... | 1977 | 407202 |
| the identification by isoenzyme patterns of two distinct strain-groups of trypanosoma cruzi, circulating independently in a rural area of brazil. | culture forms of 17 trypanosoma cruzi stocks, primarily isolated from a rural area of endemic chagas disease at são felipe, bahia, brazil, were compared by the electrophoretic patterns of six enzymes: aspartate aminotransferase, alanine aminotransferase, glucose-6-phosphate dehydrogenase, malate dehydrogenase (decarb-oxylating) (nadp+), glucosephosphate isomerase and phosphoglucomutase. two markedly distinct combinations of isoenzyme patterns were seen, justifying the arrangement of the 17 stock ... | 1977 | 407674 |
| passive immunization of mice against trypanosoma cruzi using convalescent mouse serum. | groups of 10 mice were infected by the s.c. injection of blood trypomastigotes of trypanosoma cruzi, strains "y" and "tulahuen". they were injected i.p. with convalescent mouse-anti-t. cruzi serum (cmats) at various times. a single dose of cmats (y) was most effective when injected one day after homologous infection -31 of 40 mice survived the infection, whereas all controls, both untreated and injected with normal serum, died. cmats was significantly less effective in treating mice infected wit ... | 1977 | 407684 |
| suppression of cell-mediated immunity in experimental chagas' disease. | the effect of acute infection with the tulahuén strain of trypanosoma cruzi on the cellular immune response in swiss mice was studied. mice were immunized with either freund's complete adjuvant or oxazolone, a skin sensitizing agent, and subsequently skin-tested with either bcg protoplasm or oxazolone to detect delayed hypersensitivity. depression of the response to these antigens was observed in infected mice during the stage of marked parasitemia. mice which were responsive to oxazolone before ... | 1977 | 407736 |
| search for trypanosoma rangeli in endemic areas of trypanosoma cruzi in argentina and brazil. | because the presence of trypanosoma rangeli in argentina and brazil has not been confirmed, a serach was carried out in man and triatomine insects. fifty-nine of 207 persons (28.5%) were infected when studied with one or more xenodiagnosis (40 triatoma infestans/xenodiagnosis); 0.1% to 13% of 7,821 bugs' feces and 4% of 875 dissected midguts showed t. cruzi in giemsa stained smears. one of 6,980 hemolymph samples and 1/875 salivary glands showed few flagellates which may have originated in the g ... | 1977 | 407809 |
| [natural infection of calomys musculinus (rodentia, cricetidae) by trypanosoma cruzi]. | in the rural area of las higueras, río cuarto, córdoba, argentina, a trypanosoma was isolated from a wild rodent (c. musculinus). the trypanosome was classified as trypanosoma cruzi because of the following characteristics: morphology as described by hoare for the schizotrypanum sub-genus: thin shaped, pointed back end, nucleus placed approximately in the middle of the body, prominent and subterminal kinetoplast and short free flagellum. the size measurements were as follows: total lengh 22.02 + ... | 1977 | 408884 |
| [experimental study on the resistance of a strain of trypanosoma cruzi to bay 2502]. | | 1977 | 408896 |
| [pathogenesis of chagas' disease. special emphasis on the chronic aspect]. | | 1977 | 410076 |
| [leukocyte migration inhibition with soluble and particulate antigens (author's transl]. | studies were carried out with the leukocyte migration inhibition tests using soluble and particulate antigens (ppd, somatic antigens of t. cruzi, m. bovis (bcg) and leukocytes from patients sensitized to ppd and patients with chronic chagas' disease. the results obtained showed that the stimulatory capacity of particulate antigens are greater than that of soluble antigens. | 1977 | 410077 |
| protection against challenge of mice experimentally infected with trypanosoma cruzi. | | 1977 | 410127 |
| carlos chagas and chagas' disease. | | 1977 | 410319 |
| fixation of trypanosomatids for electron microscopy with the glutaraldehyde-tannic acid method. | epimastigotes from trypanosoma cruzi and promastigotes from herpetomonas samuelpessoai were fixed with glutaraldehyde-tannic acid. different concentrations of tannic acid were tested. with this technique the cellular membranes appear in negative contrast offering the same aspect as seen in cells fixed in glutaraldehyde only without post-fixation in osmium tetraoxide. an electron-dense deposit appears on the surface which possibly represents positively charged groups. the structure of the sub-pel ... | 1977 | 411272 |
| an endemic focus of trypanosoma cruzi infection in a subhuman primate research colony. | leishmanial forms of trypanosoma cruzi with multifocal pseudocysts were identified in a deceased adult female rhesus monkey (macaca mulatta) that had been maintained in an outdoor primate colony in southern texas. subsequent clinical investigation of the remainder of the colony identified 20 additional monkeys seropositive for t cruzi. concurrent epizootiologic surveys confirmed the presence of sylvatic reservoirs and triatomine vectors of t cruzi infection and identified a natural t cruzi trans ... | 1977 | 411770 |
| pteridines. 41. synthesis and dihydrofolate reductase inhibitory activity of some cycloalka[g]pteridines. | a number of homologous 2,4-diaminocycloalka[g]pteridines varying in ring size from 5 to 15 were prepared by (a) condensation of aminomalononitrile tosylate with alpha-oximinocycloalkanones, deoxygenation of the resulting 2-amino-3-cyanocycloalka[b]pyrazine 1-oxides, and guanidine cyclization; (b) guanidine cyclization of the above pyrazine 1-oxides to give 2,4-diaminocycloalka[g]pteridine 8-oxides, followed by deoxygenation; or (c) condensation of 2,4,5,6-tetraaminopyrimidine with a cycloalka-1, ... | 1977 | 411935 |
| trypanosoma cruzi infection in mice chronically infected with toxoplasma gondii. | | 1977 | 412940 |
| [trypanosoma cruzi: the action of specific rabbit immune serums on the growth of epimastigotes in diphasic media]. | | 1977 | 413180 |
| [serologic diagnosis of human trypanosoma cruzi infection. comparative study of complement fixation, immunofluorescence, hemagglutination and floculation tests in 3,624 serum samples]. | | 1977 | 413181 |
| tissue reacting ig in children parasitaemic with trypanosoma cruzi. | | 1977 | 413220 |
| immunoprophylaxis against chagas' disease. | | 1977 | 413341 |
| experiments on immunoprophylaxis against chagas' disease. | | 1977 | 413342 |
| immunofluorescent vascular pattern due to evi antibody of chagas' disease. its diagnostic value. | one hundred fifty-six of 1,250 sera from patients with presumed connective tissue and related diseases showed vascular staining on mouse liver cryostat sections when they were routinely checked for antinuclear factor by the indirect immunofluorescence test. in a third of the cases, the vascular immunofluorescent pattern was given by the evi antibody reacting with the plasma membrane of striated muscle fibers and endothelial cells, as has been recently described to occur in chagas' disease. this ... | 1978 | 413431 |
| a precipitin test for the diagnosis of human abdominal angiostrongyliasis. | a precipitin reaction was observed when sera of cotton rats infected either naturally or experimentally with angiostrongylus costaricensis were tested by gel double-diffusion against sera obtained from three biopsy-confirmed human cases of a. costaricensis. with immunoelectrophoresis, the antigen was demonstrated in the serum of infected rats. the antibody in the human serum was mostly of the igg type. no cross reactions were seen with sera from individuals infected with the common intestinal he ... | 1977 | 413446 |
| trypanosoma cruzi: uptake and intracellular fate in normal and activated cells. | | 1977 | 413447 |
| trypanosoma cruzi: nifurtimox's effect on infectivity. | | 1977 | 413732 |
| isolation and properties of flagella of trypanosomatids. | a procedufe is described for the isolation of flagella of crithidia fasciculata, herpetomonas samuelpessoai and leishmania tarentolae in a highly purified state and giving reasonably good yield. the 3 types of flagella give a similar electrophoretic pattern of proteins. it is shown that h. samuelpessoai and, to a lesser extent, c. fasciculata flagella confer protection against trypanosoma cruzi infection. | 1977 | 413911 |
| exacerbation of experimental trypanosoma cruzi infection in mice by concomitant malaria. | the effect of malaria on the chronic phase of chagas' disease was investigated in mice. the animals were given plasmodium bergheri-infected red blood cells 2 to 12 months after their initial inoculation with trypomastigotes of 3 different strains of trypanosoma cruzi (y. cl and gilmar). in all the experiments carried out with one of the strains (cl), a somewhat variable but always considerable percentage of mice (average 39%) relapsed in to the acute phase of chagas' disease. this relapse was ch ... | 1977 | 413912 |
| antigens of the subcellular fractions of trypanosoma cruzi. ii. flagellar and membrane fraction. | a method for isolation of membrane and flagellar fractions from trypanosoma cruzi epimastigotes, and descriptions of their ultrastructural characteristics and antigenic activity are presented. electron-microscopic observation revealed that the membrane fraction resembled vesicles, as occurs frequently when the pressure-depressure method is used for cell disruption. the flagellar fraction revealed flagella and some membranes, both related and unrelated to the flagellar structure. flagellar and me ... | 1977 | 413914 |
| [congenital chagas' disease in the triângulo mineiro (report of a case)]. | | 1977 | 414341 |
| the sensitivity of culture methods to detect experimental infections of trypanosoma cruzi and comparison with xenodiagnosis. | | 1977 | 414342 |
| [the course of trypanosoma cruzi infection in nonsplenectomized spe rats with and without haemobartonella muris infection (author's transl)]. | rats were infected with h. muris from the first to the second week of their life. four weeks later 20 of the infected rats were inoculated with 1.5 x 10(5) t. cruzi. control animals were given the same number of parasites. in both experimental groups no hemoflagellate could be seen in blood smears four weeks after infection. the comparison of the course of infection of t. cruzi in rats with and without h. muris was made using t- and wilcoxon-test for pair differences. rats with h. muris infectio ... | 1977 | 414392 |
| cell-mediated immunity in chagas' disease: alterations induced by treatment with a trypanocidal drug (nifurtimox). | peripheral leucocyte migration inhibition (lmi) with trypanosoma cruzi-specific antigens, measured as a migration index (mi), was studied in chronic chagas' disease patients. the mi of untreated patients with polymerized antigens from culture forms (epimastigotes) of t. cruzi was significantly lower than that of controls. in contrast, when chronic chagas' patients were treated with nifurtimox, 10 mg/kg/day for 2 months, the mi was not different from control values. treated and untreated patients ... | 1977 | 414867 |
| trypanosoma cruzi in the cerebrospinal fluid during the acute stage of chagas' disease. | | 1978 | 415236 |
| antibody dependent cell-mediated cytotoxicity against trypanosoma cruzi. | this paper describes in vitro antibody dependent cytotoxicity against trypanosoma cruzi epimastigotes by normal mouse splenic lymphocytes. cytotoxicity was expressed as the percentage reduction in the number of motile parasites upon incubation with lymphocytes at 37 degrees c in a defined medium. failure of the non-motile parasites to regain motility and their ensuing degeneration of 28 degrees c in liver infusion tryptose (lit) medium confirmed loss of motility as a criterion of cytotoxicity. i ... | 1977 | 415286 |
| recovery from heart norepinephrine depletion in experimental chagas' disease. | norepinephrine (ne) was assayed fluorimetrically in the hearts of rats killed at different periods after inoculation with the y strain of trypanosoma cruzi. the levels of cardiac ne which, as reported previously, were not measurable 18, 22, and 32 days after inoculation became detectable at day 48. at day 70 these levels were around 50% of the control values and at day 97 normal values were attained in most animals. these results strongly suggest that sympathetic nerve fibers are destroyed in th ... | 1978 | 415626 |
| immunization of mice against infection with trypanosoma cruzi. cross-immunization between five strains of the parasite using freeze-thawed vaccines containing epimastigotes of up to five strains. | | 1978 | 415993 |
| the effect of acute infection by trypanosoma cruzi upon the response of mice to sheep erythrocytes. | | 1977 | 416482 |
| [experimental study on the therapeutic action of ro 7-1051 on infections caused by different strains of trypanosoma cruzi]. | | 1977 | 416483 |
| the primary isolation by haemoculture of trypanosoma (schizotrypanum) cruzi from animals and from man. | | 1978 | 416524 |
| comparative xenodiagnosis with three triatomine species of different hosts with natural and experimental chronic infections with trypanosoma (schizotrypanum) cruzi. | simultaneous xenodiagnosis was made of 29 patients with chronic chagas's disease, six naturally infected opossums (didelphis azarae) and of a rhesus monkey with an experimental chronic infection with trypanosoma (schizotrypanum) cruzi (peru strain). patients and opossums were from an endemic area in the state of bahia, brazil, where panstrongylus megistus is the sole domiciliary vector of the disease to man. various instars of p. megistus triatoma infestans and rhodnius prolixus were used. the p ... | 1978 | 416525 |
| [spontaneous infection of marmoset-monkeys (sanguinus oedipus) with a trypanosoma cruzi-like-strain, springer 1975. isolation and identification (author's transl)]. | a trypanosoma cruzi-like strain was isolated from marmoset monkeys originating from columbia. the frequency of affected monkeys was up to 32.7%. the parasite density was 10-40 trypomastigote forms per mililiter. monkeys, rats, mice and other small rodentia were found to be susceptible. clinical symptoms or histopathological findings could not be acertained with these trypanosomes even after a 2 1/2 year observation period. the strain behaved as an apathogen in experimental animals. the parasitae ... | 1977 | 416623 |
| [evaluation of the efficacy of nifurtimox in chronic human chagasic infection by using xenodiagnosis (author's transl)]. | | 1977 | 416834 |
| [diurnal and nocturnal positivity of the xenodiagnosis in a patient with chronic chagasic infection and permanent parasitemia (author's transl)]. | | 1977 | 416835 |
| isozymic heterogeneity of trypanosoma cruzi in the first autochthonous patients with chagas' disease in amazonian brazil. | | 1978 | 417267 |
| the effect of an immune rna (rnai) against trypanosoma cruzi infection in mice. | immune ribonucleic acid (rnai) was extracted with phenol from the spleen of mice immunized with the avirulent pf strain of trypanosoma cruzi. these preparations were able to induce immunocompetent cells to answer as a secondary response to later challenges with the virulent y strain of the same parasite. the preparations of rnai were"immunogenic" and free of proteins. the rnai preparations were sensitive to pancreatic rnaase and lost their immune effect when pretreated with this enzyme. the inje ... | 1978 | 417440 |
| [early immunological aspects in rats infected by trypanosoma cruzi. i. agglutination and immunofluorescence tests with antigen of trypomastigotes recovered in successive days of the infection (author's transl)]. | from rats infected with the y strain of t. cruzi trypomastigotes were recovered in successive days to be used in agglutination tests (at) and in direct immunofluorescence tests (dit). titres obtained in at reached 1/640 with trypomastigotes in the fourth and fifth days, decreased to 1/160 in the sixth day and were negative in the seventh day; in the tenth day the titers were again up to 1/40 and reached 1/320 in the eleventh day. the dit were negative with trypomastigotes in the fourth and fifth ... | 1978 | 418466 |
| presence of immunoglobulins on the surface of bloodstream trypanosoma cruzi. capping during differentiation in culture. | | 1977 | 418490 |
| effect of sex of mice in relation to their response to immunization with vaccines prepared from trypanosoma cruzi. | | 1978 | 418539 |
| mode of action of the 2-nitroimidazole derivative benznidazole. | using cultures of trypanosoma cruzi cells in a liquid medium, the mode of action of the 2-nitroimidazole derivative benznidazole was examined. benznidazole inhibits protein synthesis and rna synthesis in t. cruzy. the dna synthesis is only slightly decreased and no effect on the aerobic respiration of t. cruzi was observed. | 1978 | 418744 |
| the effect of nifurtimox on experimental infections with trypanosomatidae other than trypanosoma cruzi. | the influence of nifurtimox on trypanosoma brucei, t. congolense, t. equinum, t. equiperdum, t. evansi, t. gambiense, t. lewisi, t. rhodesiense, t. vivax, leishmania donovani and l. tropica has been studied in animals and in vitro. the drug was active against all of them but there are considerable differences in sensitivity of the various species as well as of different strains of t. rhodesiense. for the treatment of t. rhodesiense a single high dosage was more efficient than the same dose divid ... | 1979 | 506553 |
| mortality and other parameters of concomitant infections in albino mice: the schistosoma-toxoplasma model. | inocula of s. mansoni and t. gondii well tolerated by albino mice when given singly, were given concomitantly to groups of animals. few notable effects were observed when toxoplasmosis preceded schistosomiasis, but the s. mansoni leads to t. gondii model, with an interval of 59 days between infections, led to massive mortality, great weight loss and a striking splenomegaly. these findings are very similar to data from previous work on the s. mansoni leads to trypanosoma cruzi model. the authors ... | 1977 | 610025 |
| [influence of the cryopreservation at nitrogen temperature on the vaccinic ability of the "pf" strain of trypanosoma cruzi (author's transl)]. | the cryopeservation of the "pf" strain of t. cruzi at -196 degree c has not changed its effectiveness in protecting mice against a posterior infection with a virulent trypanosoma cruzi strain. the cryopeservation technique utilized seems to be efficient and of practical and rapid examination. | 1978 | 684265 |
| regional variations in calculated diastolic wall stress in rat left ventricle. | principal radii of curvature and wall thickness were measured at the apex and three other loci on the free wall of the left ventricle in 16 rat hearts that had been fixed at end-diastolic pressure. the law of laplace was applied to calculate the mean tensile stress at each locus. no significant variation was found among the nonapical loci, but apical stress was approximately twice the stress calculated for any nonapical locus. the higher stresses that must be borne by the apex may be a predispos ... | 1978 | 686193 |
| [achalasia caused by infiltrating carcinoma of the esophagus. clinical case and physiopathological considerations]. | a case of symptomatic esophageal achalasia due to a slowly growing neoplastic infiltration of the esophageal wall by a mammary indifferentiated carcinoma operated ten years before is presented. at admission, the clinical history and the endoscopic appearance of the esophageal lumen and mucosa led to the diagnosis of idiopathic achalasia, while the esophageal manometry showed a rather long high pressure zone (6--8 cm), which did not relax with deglutition. barium study confirmed the length of the ... | 1978 | 692894 |
| serum ige levels in amoebiasis. | elevated levels of serum immunoglobulin e (ige) are found in many parasitic diseases. in order to determine the influence of entamoeba histolytica on ige levels of clinically symptomatic patients with intestinal amoebiasis, a controlled study was performed. there was no difference between the serum ige values from brazilian patients with amoebiasis and those from age-, race- and sex-matched normal brazilian controls. based also on our previous similar studies on giardiasis and chagas' disease, w ... | 1978 | 709800 |
| mechanisms of natural resistance to trypanosomal infection. role of complement in avian resistance to trypanosoma cruzi infection. | the natural resistance of chickens to trypanosoma curzi infection and the capacity of their sera to lyse blood (trypomastigote) forms of the parasite in vitro were found to be complement-dependent phenomena. parasites given intravenously to decomplemented chickens were detectable in their bloodstream for at least 24 h post-infection, whereas in untreated animals they became undetectable after 1 min (and destroyed flagellates were observed). one millilitre of serum had the capacity to lyse as man ... | 1976 | 765264 |
| topographical differences in the distribution of surface coat components and intramembrane particles. a cytochemical and freeze-fracture study in culture forms of trypanosoma cruzi. | a regional specialization of the cell surface of t. cruzi culture forms was found at the cytostome as a localized thick surface coat rich in carbohydrate-containing components. the prominent surface coat was located over a region of the plasma membrane where intramembranous particles were exceedingly low in number. in turn, the particle-poor region was related to specialized submembrane fibrils not present under other regions of the plasma membrane. the cystostome region provides a striking exam ... | 1976 | 770483 |
| use of 125i-labelled albumin for the detection and measurement of delayed-hypersensitivity reactions in the mouse. | accumulation of 125i-labelled albumin in the mouse hind foot has been used to measure reactions of delayed hypersensitivity in the mouse. the assay is technically simple and permits use of groups of 10 or more animals. its sensitivity and reproducibility have been demonstrated by comparing sensitized with non-sensitized animals in two antigen systems (p.p.d. and an extract of trypanosoma cruzi). | 1976 | 774994 |
| trypanosoma cruzi: mechanism of entry and intracellular fate in mammalian cells. | the mode of entry and intracellular fate of epimastigotes and trypomastigotes of trypanosoma cruzi in cultured cells was studied. electron microscopic observations indicated the uptake by phagocytosis of both forms into mouse peritoneal macrophages and of trypomastigotes and transition forms into other cultured cell types. in each instance the organisms were initially surrounded by a plasma membrane-derived phagosome. trypsin and chymotrypsin treatment of the macrophages completely abolished att ... | 1976 | 775012 |
| strain-dependent thermosensitivity influencing intracellular differentiation of trypanosoma cruzi in cell culture. | temperature strongly influenced morphogenesis of intracellular trypomastigotes in cell culture infected with 2 different strains of t. cruzi. with the gilmar strain the amastigote-to-trypomastigote differentiation readily occurred at 33 and 37 c, whereas the cl strain differentiation took place at 33c but was inhibited at 37 c. the possiblity of this selective thermosensitivity resulting from mutational adaptation of the parasite is discussed. | 1976 | 775065 |
| comparative kinetics of arginine and lysine transport by epimastigotes and trypomastigotes from two strains of trypanosoma cruzi. | three-day-old cultures of y and mr strains of trypanosoma cruzi ahd a higher rate of lysine and arginine uptake than 10-day cultures. amino acid uptake by cells of the mr strain was consistently higher than that of the y strain. flagellates separated on deae-cellulose columns have normal structure, motility, and infectivity; they have the higher rates of lysine and arginine uptake than the original 3- and 10-day cultures. in addition passage through deae-cellulose columns modified the kinetic be ... | 1976 | 775066 |
| trypanosoma cruzi: effects of rhodnius prolixus extracts on in vitro development. | | 1976 | 775590 |
| ultrastructural observations of trypanosoma cruzi in a liquid medium: the kinetoplast-mitochondrion in division forms. | | 1976 | 776884 |
| a simple monophasic medium for axenic culture of hemoflagellates. | an inexpensive, easily prepared monophasic medium which supports continuous culture of leishmania donovanì (approximately 4 x 10(7) cells/ml/day 5) and l. tarentolae (approximately 3.5 x 10(7) cells/ml/day 5) promastigotes has been developed. this medium, designated ho-mem, is a modified eagle's minimal essential medium with spinner's salts and 10% fetal calf serum. epimastigotes of trypanosoma cruzi, costa rica strain, also grew well in ho-mem (approximately 2 x 10(7) cells/ml/day 5) but cells ... | 1976 | 778371 |
| circulating antibodies in cutaneous leishmaniasis: their detection by immunofluorescence. | with the use of an indirect fluorescent antibody (ifa) test and freeze-dried antigens, circulating antibody titres of 1 in 100 or above to leishmania tropica leptomonad forms were detected in 66-6% and 76-2% of 18 patients with active and 21 patients with healed cutaneous leishmaniasis, respectively. cross-reactivity was observed with l. donovani and trypanosoma cruzi antigens. five out of 15 patients with active pulmonary tuberculosis had antibodies to l. tropica major, suggesting a possible cr ... | 1975 | 779154 |
| a fluorescent character distinguishing strains of trypanosoma (schizotrypanum) cruzi isolated from animals. | | 1975 | 779157 |
| a lipopeptidophosphoglycan from trypanosoma cruzi (epimastigota). isolation, purification and carbohydrate composition. | a lipopeptidophosphoglycan was extracted from epimastigote forms of trypanosoma cruzi by phenol (44%) treatment of sonicated cells. the substance was purified from other glycoproteins and nucleic acid as follows: ethanol fractionation. bio-gell p-150 column chromatography in the presence of 0.1% sodium dodecyl sulfate, extraction with chloroform/methanol/water (10 : 10 : 3) and precipitation of the pure compound by methanol. the substance migrated as a single band in sodium dodecyl sulfate-polya ... | 1976 | 782545 |
| localization of peroxidase activity in trypanosoma cruzi microbodies. | electron microscopic observation of trypanosoma cruzi epimastigotes reveals the presence of microbody-like structures (microperoxisomes) in which 3,3'-diaminobenzidine (dab) is peroxidized to electron-opaque material. the role of peroxidase in dab peroxidation is supported by the enzyme demonstration in disrupted epimastigotes and the microbody-containing cell fractions. | 1976 | 782907 |
| cytochrome p-450 and drug metabolism in trypanosoma cruzi: effects of phenobarbital. | the epimastigotes of trypanosoma cruzi hydroxylate drugs at substantial rates. the activity, which is of the mixed-function oxidase type, is increased by phenobarbital and is inhibited by co, skf 525-a, and metyrapone. the hydroxylation is paralleled by increases in free and membrane-bound ribosomes. | 1976 | 785602 |
| species of the subgenus schizotrypanum other than trypanosoma cruzi and their potential usefulness in the laboratory. | | 1976 | 785723 |
| letter: trypanosomiasis cruzi in mice with trichinosis. | | 1976 | 785726 |
| a simple method of tracking mammals and locating triatomine vectors of trypanosoma cruzi in amazonian forest. | a simple method of tracking mammals from capture points to their refuges was developed to study trypanosoma cruzi reservoir-vector associations in belém forest. a device containing a spool of fine line was carried by released mammals so that the line unwound under minimum tension as the animal proceeded and could be followed the day after release. twenty-three triatomine bugs of five species were found with 3 of 15 mammals retrieved. | 1976 | 786052 |
| uptake of fetal proteins by trypanosoma cruzi immunofluorescence and ultrastructural studies. | | 1976 | 786918 |
| electron microscopic study of trypanosoma cruzi periplast in tissue cultures. i. number and arrangement of the peripheral microtubules in the various forms of the parasite's life cycle. | in an electron microscopic study, counts of peripheral microtubules were made in spheromastigotes and trypomastigotes in tissue cultures of embryonic heart muscle cells. in interphase spheromastigotes there were, at the level of the nucleus, approximately 114 microtubules; in dividing forms, there were approximately 222. in trypomastigotes, the number of microtubules varied according to the level of the section--there were fewer than 40 tubules in the pointed ends of an organism, while in the ce ... | 1976 | 787513 |
| [effects of gamma rays on trypanosoma cruzi. ii antibodies in mice inoculated with culture forms]. | | 1976 | 788112 |
| proline metabolism in trypanosoma cruzi epimastigotes. | | 1976 | 789007 |
| [vaccination with live, non-virulent trypanosoma cruzi vaccine in man. a 5-year follow up study the 1st 2 cases]. | | 1976 | 790483 |
| glucose metabolism, growth and differentiation of trypanocoma cruzi. | | 1976 | 790488 |
| patterns of development of trypanosoma cruzi in the embryonated chicken egg. | blood forms of four strains of trypanosoma cruzi were inoculated on the chorioallantois or intra-yolk sac in seven to nine day embryonated chicken eggs which were reincubated thereafter at 37-5-39-0 degrees c or at 32-34 degrees c. there were no significant differences between strains when total rates of infected eggs were compared. however, the highest proportions of positives, for every strain, were found among eggs inoculated into the yolk sac and reincubated in the lower temperature range. t ... | 1976 | 793546 |
| the enzymic hydrolysis of acetyl-coenzyme a by trypanosomatid flagellates. | | 1976 | 793894 |
| loss of infectivity of mycobacterial and protozoal exoplasmic parasites after exposure in vitro to the polyenzymic cocktail 'pigo'. | | 1976 | 793902 |
| demonstration of kinetoplast dna in trypanosomidae by using a fluorescent compound employed in human cytogenetics. | | 1976 | 794608 |
| [lipid composition of trypanosoma cruzi]. | | 1976 | 794622 |
| [cryopreservation of trypanosoma cruzi in examples of triatoma infestans experimentally infected]. | | 1976 | 794979 |
| [cellular pathology]. | | 1976 | 800311 |
| [alpha-hydroxy-acid dehydrogenase activity in trypanosoma cruzi]. | crude extracts of culture forms of epimastigotes, tulahuén strain, showed activity to catalyze the reaction alpha-ketocid in equilibrium alpha-hydroxyacid linked to nicotinamide adenine dinucleotide (nad). the enzyme utilizes the following substrates: alpha-ketobutyrate, alpha-ketoisovalerate, alpha-keto-beta-methylvalerate, alpha-ketocaproate, alpha-ketoisocaproate, alpha-ketoglutarate and pyruvate. kinetics for the three last substrates were of the michaelian type. for the other ketocids, curv ... | 1976 | 801103 |
| the pyruvate kinase of trypanosoma cruzi. | | 1976 | 802627 |