| histoplasma capsulatum endophthalmitis after cataract extraction. | a 60-year-old white man from an area endemic for histoplasma capsulatum presented with a vitreous wick, hypopyon iritis, and dense vitreitis 2 months after removal of an anterior chamber intraocular lens (iol) for chronic iritis. a diagnostic vitrectomy was performed and h. capsulatum was cultured and identified 2 weeks later. despite intravitreal and intravenous amphotericin as well as repeat vitrectomies, the inflammation worsened and the eye was removed. results of histopathologic examination ... | 1990 | 2326010 |
| comparison of fluconazole with amphotericin b in treatment of histoplasmosis in normal and immunosuppressed mice. | the activities of fluconazole and amphotericin b against histoplasma capsulatum were investigated. the minimum inhibitory concentrations ranged from 0.12 to 0.47 microgram/ml for amphotericin b and from 16 to 250 micrograms/ml for fluconazole. fluconazole given orally twice a day for 6 consecutive days compared favorably with amphotericin b given intraperitoneally once every other day for a total of six doses in the treatment of histoplasmosis in normal and leukopenic mice. | 1990 | 2330487 |
| in vitro and in vivo activities of sch 39304, fluconazole, and amphotericin b against histoplasma capsulatum. | the antifungal activities of amphotericin b and two triazoles, sch 39304 and fluconazole, were tested against histoplasma capsulatum. in this study sch 39304 compared favorably with amphotericin b in treating histoplasmosis in normal and leukopenic mice, whereas fluconazole was much less active. the differences in the efficacies of the triazoles appeared to be due to differences in their pharmacokinetics and the dosage schedule that was used. for amphotericin b there was a good correlation betwe ... | 1990 | 2344160 |
| disseminated histoplasmosis presenting as penile ulcer. | a 59-year-old chinese man presented with a three month history of penile ulcers and abdominal pain. syphilis and a gastric malignancy were diagnosed clinically. biopsy of the genital and stomach ulcers revealed histiocytic granulomata containing numerous intracellular fungal bodies. histoplasma capsulatum was subsequently cultured from the penile ulcer. antifungal therapy was instituted. however the patient's general condition deteriorated rapidly and he died within two weeks of admission. a pos ... | 1990 | 2344324 |
| disseminated histoplasmosis in aids. clinicopathologic features in seven patients from a non-endemic area. | although the clinical and epidemiologic features of progressive disseminated histoplasmosis (pdh) in the acquired immunodeficiency syndrome (aids) have been well described, the pathologic and pulmonary aspects remain to be fully defined. a retrospective review of three patients and a prospective study of four more with pdh and aids recently admitted to an inner city hospital in a non-endemic area were used to elucidate these features more fully. all patients were men aged 23 to 46 years, born in ... | 1990 | 2348942 |
| development of a genetic transformation system for histoplasma capsulatum: complementation of uracil auxotrophy. | we have developed a simple and efficient transformation system for the dimorphic fungus histoplasma capsulatum. mutants of h. capsulatum defective in orotidine-5'-monophosphate pyrophosphorylase were transformed to prototrophy by the cloned ura5 gene of the filamentous fungus podospora anserina. abortive and mitotically stable transformants were obtained. the stable transformants had integrated copies of the plasmid, some in tandem head-to-tail orientation. free plasmid identical to the transfor ... | 1990 | 2381417 |
| disseminated histoplasmosis presenting as a non-healing tongue ulcer. | a 51-year old man presented with a persistent tongue ulcer, fever, cervical lymphadenopathy and hepatomegaly. the diagnosis was initially thought to be tuberculosis. this led to the initiation of antituberculous chemotherapy to which the patient failed to respond. the correct diagnosis of histoplasmosis was made after the detection of histoplasma capsulatum on further review of the tongue ulcer biopsy specimen. he responded to treatment with amphotericin b. | 1990 | 2392708 |
| therapeutic effect of the triazole bay r 3783 in mouse models of coccidioidomycosis, blastomycosis, and histoplasmosis. | a new triazole, bay r 3783, was compared with ketoconazole, itraconazole, and fluconazole, which were given via the alimentary tract at three dosages, and amphotericin b, which was given at 1 mg/kg intraperitoneally, in murine models of the systemic mycoses coccidioidomycosis, histoplasmosis, and blastomycosis. in a pulmonary coccidioidomycosis model, bay r 3783, fluconazole, and itraconazole were essentially equally efficacious and more active than ketoconazole in protecting mice against death; ... | 1990 | 2393272 |
| preparation of species-specific murine monoclonal antibodies against the yeast phase of paracoccidioides brasiliensis. | a panel of four murine monoclonal antibodies showing species specificity for the yeast phase of the pathogenic dimorphic fungus paracoccidioides brasiliensis was produced by using a modification of the standard monoclonal antibody technology. this involved the use of the immunosuppressive drug cyclophosphamide to suppress the immune response of test animals to fungi showing cross-reactivity, i.e., to histoplasma capsulatum. one monoclonal antibody, p4, which had a high titer by enzyme-linked imm ... | 1990 | 2394802 |
| surgical implications of sclerosing mediastinitis. a report of six cases and review of the literature. | sclerosing mediastinitis is an uncommon disease associated with a multiplicity of clinical syndromes. the cause of this disorder is probably an abnormal fibroproliferative response to an inflammatory stimulus, most commonly a granulomatous infection secondary to histoplasma capsulatum. the pathophysiology of this disease is predicated on the encasement of mediastinal vital organ structures within a dense fibrotic mass. this mass appears to emanate from an invasive chronic inflammatory process ca ... | 1990 | 2404701 |
| subretinal neovascularization after experimental ocular histoplasmosis in a subhuman primate. | subretinal neovascularization was demonstrated in a subhuman primate eye (macaca speciosa) one year after the internal carotid injection of yeast-phase histoplasma capsulatum. in this animal model of ocular histoplasmosis, initial injection of viable h. capsulatum results in the development of self-limiting acute multifocal choroiditis. acute lesions resolve within six months, forming chorioretinal scars that are clinically similar to human histo spots. detailed ultrastructural study of a peripa ... | 1985 | 2411140 |
| detection of an antigenic cell wall layer in histoplasma capsulatum. an immunoelectron microscopic study. | histoplasma capsulatum yeast cells have been studied by immunoelectron microscopy using rabbit polyclonal antisera and a biotin-avidin-peroxidase detection system. an antigenic surface layer has been visualized in the cell wall of immunostained organisms. this layer was not seen in samples prepared by standard electron microscopic methods or in negative controls used with the immunocytochemical technique. without immunostaining the cell wall of histoplasma appeared almost transparent. in contras ... | 1985 | 2412520 |
| disseminated bilateral chorioretinitis due to histoplasma capsulatum in a patient with the acquired immunodeficiency syndrome. | a 31-year-old white male homosexual was healthy until march 1984, when he developed pneumocystis carinii pneumonia, which resolved with treatment. in april 1984 he developed fever, followed by hepatosplenomegaly, headaches, blurred vision, pancytopenia and pulmonary infiltrates. on june 11, intracytoplasmic yeast were noted within leukocytes on a peripheral blood smear, and amphotericin b was started. the patient developed progressive respiratory and renal insufficiency and died on june 13, 1984 ... | 1985 | 2413418 |
| evaluation of cross-reactions in histoplasma capsulatum serologic tests. | cross-reactivity in histoplasma serologic tests was evaluated by using sera from patients with histoplasmosis and other infections. serum samples from 127 of 134 (95%) patients with histoplasmosis were judged positive by complement fixation tests, and 121 (90%) showed h bands, m bands, or both by immunodiffusion. of these 134 patients, cross-reactions were seen to blastomyces dermatitidis in 53 patients (40%), to coccidioides immitis in 20 patients (16%), and to aspergillus fumigatus in 3 patien ... | 1986 | 2420821 |
| immunocytochemical staining of histoplasma capsulatum at the electron microscopic level. | rabbits were immunized with histoplasmin emulsified in freund's complete adjuvant. antibody raised in these rabbits was exposed to histoplasma capsulatum yeast cells, either in tissue culture medium, or after in vitro or in vivo phagocytosis by mouse macrophages. the sites of antibody binding were identified using an immunoperoxidase technique. at least two sites of antibody binding were identified, one to the fungal cell wall and the other to the outer cell membrane. within 6 h after phagocytos ... | 1986 | 2429184 |
| development and characterization of histoplasma capsulatum-reactive murine t-cell lines and clones. | experimental studies have suggested that antigen-specific t lymphocytes are important mediators of resistance to infection with the pathogenic fungus histoplasma capsulation. to gain a better understanding of the role of t lymphocytes, we developed murine t-cell lines and clones that recognized histoplasma antigens. these t cells were of the helper/inducer phenotype (thy-1.2+ lyt-1+ l3t4+ lyt-2-) and exerted multiple immunological functions. t-cell lines and 12 clones proliferated vigorously in ... | 1986 | 2430887 |
| the value of calcofluor white in the diagnosis of mycotic and acanthamoeba infections of the eye and ocular adnexa. | in search of an easy and quick method for the diagnosis of fungal and acanthamoeba infections of the eye, the authors have used calcofluor white (cfw), a fluorescent brightener with marked affinity for chitin and cellulose which are present in the cell walls of fungi and cysts of acanthamoeba. paraffin-embedded tissues containing mycotic organisms were stained with cfw and examined under a fluorescent microscope. several fungi such as candida sp, aspergillus sp, phycomycetes, fusarium solani, hi ... | 1987 | 2436125 |
| determination of viability of histoplasma capsulatum yeast cells grown in vitro: comparison between dye and colony count methods. | the viability of histoplasma capsulatum yeast cells grown under different conditions was determined by dye tests with eosin-y and janus green b and by colony counts of cells plated on brain-heart infusion agar supplemented with histoplasma growth factor and bovine serum albumin (bhi-sag). the test samples included cells grown on brain-heart infusion agar at 37 degrees c for 2-7 days, cells grown in glucose-cysteine broth medium for 1-31 days, and cells grown on brain-heart infusion agar for 3 da ... | 1987 | 2439674 |
| phagosome-lysosome fusion in p388d1 macrophages infected with histoplasma capsulatum. | the issue of whether or not phagocytized histoplasma capsulatum yeasts evade phagosome-lysosome fusion (p-lf) has been debated by several investigators. to resolve this problem, yet avoid drawbacks associated with the conventional assays of p-lf (electron microscopy and the acridine orange assay), we used fluorescein isothiocyanate-labeled dextran (fitc-dextran) to monitor p-lf in the macrophage-like cell line p388d1.d2. controls indicated that fitc-dextran could be used to distinguish between e ... | 1988 | 2454277 |
| cutaneous histoplasmosis in the acquired immune deficiency syndrome--a report of three cases from trinidad. | three cases are reported of patients with the acquired immune deficiency syndrome (aids) and cutaneous histoplasmosis. their initial presentation was that of a generalised maculopapular rash. two patients were bisexual males and the third was an unmarried female. the range of opportunistic infections seen in aids patients in trinidad is mentioned and clinicians are alerted to the fact that in areas endemic for histoplasma capsulatum maculopapular rash in patients with aids may suggest disseminat ... | 1988 | 2457268 |
| characterization of antigenic determinants in histoplasmin that stimulate histoplasma capsulatum-reactive t cells in vitro. | although antigen-reactive t lymphocytes play a central role in the host response to histoplasma capsulatum, little is known of the nature of histoplasma antigens recognized by these cells in vitro. employing a murine t-cell line and two clones that are reactive with histoplasmin, we examined whether activation of t cells by histoplasmin required the presence of carbohydrate or protein moieties. the approach taken was to modify carbohydrate or protein molecules in histoplasmin by chemical or enzy ... | 1988 | 2457554 |
| fusion of lysosomes with phagosomes containing histoplasma capsulatum: use of fluoresceinated dextran. | | 1988 | 2462337 |
| broncholithiasis due to histoplasma capsulatum subsequently infected by actinomycetes. | a case of broncholithiasis in which both histoplasma and actinomycotic organisms were demonstrated is presented. the etiology of broncholithiasis is discussed, with particular emphasis on the relationship between the organisms identified. | 1989 | 2472254 |
| fungi on the claws of buffalo and cow in egypt. | the frequency of occurrence of fungi in 120 claw samples of buffalo and cow from different localities at assiut governorate was determined. thirty-seven species which belong to twenty genera were collected from the two substrates and the most common genera were chrysosporium (eleven species of chrysosporium were isolated). aspergillus and scopulariopsis. from the preceding genera chrysosporium sulfureum was common on cow claws but c. merdarium was slightly dominant on buffalo claws than on cow c ... | 1989 | 2482351 |
| diagnosis and management of histoplasmosis. | histoplasmosis occurs throughout the world but is more common within the endemic areas of north america, particularly in fertile river valleys. disease manifestations range from asymptomatic infection in the normal host with low-inoculum exposure to rapidly fatal, disseminated infection in the severely immunocompromised host, emphasizing the importance of cellular immunity in defense against histoplasma capsulatum. diagnosis depends on a high index of suspicion, knowledge of the clinical and epi ... | 1989 | 2502413 |
| macrophage cell lines p388d1 and ic-21 stimulated with gamma interferon fail to inhibit the intracellular growth of histoplasma capsulatum. | histoplasma capsulatum, a facultative intracellular parasite of macrophages, grows within mononuclear cells of the p388d1 and ic-21 cell lines with a generation time comparable to that with which it grows in normal resident peritoneal macrophages (10 +/- 2 h). recombinant murine gamma interferon (rmuifn-gamma) activates p388d1 cells to express la antigens but not to inhibit the intracellular growth of h. capsulatum, alone or in combination with lipopolysaccharide. ic-21 cells also could not be a ... | 1989 | 2503448 |
| relative susceptibilities of inbred mouse strains c57bl/6 and a/j to infection with histoplasma capsulatum. | differences in the 30-day survival of histoplasma capsulatum after intravenous injection indicated that the a/j strain of inbred mouse was more resistant to experimental infection than was the c57bl/6 strain. cfu from the spleens of infected animals increased during the first week after injection but gradually declined over the next 3 weeks. the cfu per gram of tissue in the c57bl/6 animals were 10- to 100-fold higher than were those in the a/j mice during the time between 7 and 28 days after in ... | 1989 | 2509369 |
| expression of alpha- and beta-tubulin genes during dimorphic-phase transitions of histoplasma capsulatum. | recent investigations have confirmed the presence of one alpha-tubulin gene (tub1) and one beta-tubulin gene (tub2) in the dimorphic fungus histoplasma capsulatum. in the present study, northern blot (rna blot) analyses revealed multiple alpha-tubulin transcripts and a single beta-tubulin transcript in the yeast and mycelial phases of the high-virulence 217b strain and low-virulence downs strain. s1 nuclease protection assays demonstrated one initiation start site and two major stop sites for th ... | 1989 | 2546058 |
| treatment of disseminated histoplasmosis in hamsters. | a comparative study between itraconazole, ketoconazole and amphotericin b in the treatment of experimental histoplasmosis in hamsters was carried out. seventy five animals were inoculated intracardiacally with the yeast-phase of histoplasma capsulatum. they were divided in 5 groups: 1) treated with itraconazole by gavage (g) at a daily dose of 16 mg/kg; 2) treated with ketoconazole by (g) at a daily dose of 80 mg/kg; 3) treated with amphotericin b intraperitoneally (i.p.) at 6 mg/kg every other ... | 1989 | 2546081 |
| [value of fluconazole in the treatment of systemic yeast infection]. | 20 patients (18 men, 2 women), 10 of whom were hiv +, were given fluconazole (f) for either systemic candidiasis (13 cases), histoplasmosis (1), or cryptococcosis (6). the localization of the candida infections (12 c. albicans, 1 c. tropicalis), were: septicemic (2), urinary (7), bronchial (2), esophageal (5), uveal (1), soft tissue (2), and 1 undetermined localization but a positive serology (1). on day (d) 1, candidiasis patients were given an initial dose of 400 mg (for septicemia) or 200 mg ... | 1989 | 2552380 |
| variable expression of a yeast-phase-specific gene in histoplasma capsulatum strains differing in thermotolerance and virulence. | by means of differential hybridization techniques, several yeast-phase-specific dna sequences were identified in the dimorphic pathogenic fungus histoplasma capsulatum. a 1.85-kilobase (kb) hindiii fragment from one genomic clone, yps-3, hybridized to at least three distinct yeast poly(a)+ rnas of 1.3, 1.05, and 0.95 kb from the virulent strain g217b. these mrnas were not detected in mycelia. when mycelia from g217b were induced to become yeast by transfer from 25 to 37 degrees c, a process requ ... | 1989 | 2565289 |
| use of mitochondrial and ribosomal dna polymorphisms to classify clinical and soil isolates of histoplasma capsulatum. | we have developed an improved scheme for the classification of environmental and clinical isolates of histoplasma capsulatum that is based on analysis of mitochondrial dna (mtdna) and ribosomal dna (rdna). strains were initially divided into mtdna groups according to restriction digests of whole-cell dna and southern hybridization with cloned mtdna probes. strains within a mtdna class could be further grouped by polymorphisms in rdna. the majority of soil and clinical isolates from the united st ... | 1989 | 2565290 |
| dna probe for the identification of histoplasma capsulatum. | a 1.85-kilobase hindiii nuclear dna probe from histoplasma capsulatum g217b detected polymorphic restriction fragments within whole-cell dna from different clinical isolates of h. capsulatum, consistent with the previous system of classification. the probe failed to hybridize to dna from blastomyces dermatitidis, candida spp., saccharomyces cerevisiae, sepedonium chrysospermum, and chrysosporium keratinophilum under low-stringency conditions and therefore may have value as a diagnostic reagent t ... | 1989 | 2573616 |
| naturally occurring mucocutaneous histoplasmosis in a rabbit. | mucocutaneous histoplasmosis was diagnosed in a pet rabbit. a mass protruding through the anal opening was histologically composed of a densely cellular infiltrate of macrophages which expanded the anorectal submucosa. macrophages contained abundant yeast forms of fungi morphologically consistent with histoplasma capsulatum. infection appeared to be localized. histoplasmosis should be considered in the differential diagnosis of granulomatous inflammatory disease in the rabbit. | 1989 | 2584453 |
| a new presentation of disseminated histoplasmosis in a homosexual man with aids. | the clinical and histopathological features and the therapeutic response of a pustular eruption occurring in a homosexual man with acquired immune deficiency syndrome (aids) is reported. the rare cutaneous presentation consisted of mostly circumscribed, tender, tense pustules, associated with erythema, confined to the face and neck. biopsy of these lesions revealed intracellular round to oval bodies surrounded by a clear space, consistent with histoplasma capsulatum. prompt resolution was observ ... | 1989 | 2596497 |
| histoplasmosis presenting as hyperplastic gingival lesion. | a case of histoplasmosis presenting as ulcerated, hyperplastic gingiva and with no other systematic signs and symptoms is reported. the clinical presentation and the rarity of this condition led one of us to the differential diagnosis of a malignant lesion. the correct diagnosis was made after histological examination and tissue culture which yielded histoplasma capsulatum. the patient was successfully treated with ketoconazole. | 1989 | 2607474 |
| characterization of alpha and beta tubulin genes in the dimorphic fungus histoplasma capsulatum. | evidence from our laboratory indicates that microtubules are involved in the differentiation of the dimorphic, pathogenic fungus histoplasma capsulatum; therefore, we cloned the tubulin genes from a virulent strain of the organism. we report that the h. capsulatum genome contains a single alpha (tub1) and a single beta (tub2) tubulin gene rather than the more typical multigene family which is common in even the simplest eukaryotes. sequence data from these genes reveal a high degree of nucleotid ... | 1989 | 2614394 |
| mycelium-yeast transition in histoplasma capsulatum. early ultrastructural changes. | the ultrastructural changes which occur during the first 24 h of mycelium to yeast transition have been studied in the dimorphic fungus histoplasma capsulatum. a temperature shift controls mycelial to yeast transition. during the first 24 h respiratory rate, atp and cytochrome concentration fall to very low levels. ultrastructural observations showed that the plasma membrane became undulated and the cell wall lost its characteristic fibrous outer layer. at 8 h the ordered lamellar structure of t ... | 1989 | 2615784 |
| invasive histoplasmosis of the mandible. | we present a case of an 82-year-old man with a 1-year history of a slowly enlarging, nontender, exophytic oral mucosal lesion with focal ulceration of the right alveolar ridge of the mandible associated with cortical bone resorption. two consecutive biopsies revealed histoplasma capsulatum. a review of the literature confirms that this is an uncommon manifestation of this fungal disease, but must be considered in the differential diagnosis of an oral ulcerative lesion. | 1989 | 2646245 |
| community-acquired fungal pneumonias. | histoplasma capsulatum, coccidioides immitis, blastomyces dermatitidis, and cryptococcus neoformans can cause acute community-acquired pneumonia. all are soil-dwelling fungi and disturbance of contaminated soil is necessary for infection in man. each has particular epidemiologic considerations that may offer a clue to diagnosis, but the clinical presentation is not sufficiently characteristic to be of diagnostic help. infection may produce variable symptoms, ranging from asymptomatic skin test c ... | 1989 | 2652235 |
| dimorphism in histoplasma capsulatum: a model for the study of cell differentiation in pathogenic fungi. | several fungi can assume either a filamentous or a unicellular morphology in response to changes in environmental conditions. this process, known as dimorphism, is a characteristic of several pathogenic fungi, e.g., histoplasma capsulatum, blastomyces dermatitidis, and paracoccidioides brasiliensis, and appears to be directly related to adaptation from a saprobic to a parasitic existence. h. capsulatum is the most extensively studied of the dimorphic fungi, with a parasitic phase consisting of y ... | 1989 | 2666842 |
| histoplasmosis. | histoplasmosis is caused by histoplasma capsulatum, a dimorphic fungus. because histoplasmosis is usually a self-limited disease in the majority of cases, treatment often is not needed in the normal host. however, severe cases of acute pulmonary histoplasmosis require therapy. amphotericin b is considered the treatment of choice. | 1989 | 2670370 |
| [histoplasmosis, serologic follow-up]. | this paper reports the positive results obtained in the sera of 70 patients with histoplasmosis, the clinical presentation of the disease, and the possibilities for an appropriate follow-up of the clinical course of these patients by means of titers of the sera taken during the persistence of the signs and symptoms of the disease and following its resolution. the most frequent clinical presentation was acute pulmonary disease and the single m precipitation band was found in a higher number of se ... | 1989 | 2672167 |
| african histoplasmosis masquerading as carcinoma of the colon. report of a case and review of literature. | a case of african histoplasmosis primarily affecting the large bowel--a very unusual manifestation--is reported. in the absence of classical clinical features, pathognomonic of large bowel histoplasmosis, the macroscopic appearance at surgery was confused with large-bowel cancer. the diagnosis was, therefore, retrospective and based on demonstration of large yeast cells of histoplasma capsulatum var. duboisii in tissue sections of the lesions. | 1989 | 2676427 |
| genetics and molecular pathogenesis of legionella pneumophila, an intracellular parasite of macrophages. | in addition to providing a powerful approach for identifying bacterial factors required for full infectivity and disease production, genetic analysis of legionella pathogenesis should also lend critical insight into the biology of the macrophage and into the pathogenesis of other intracellular parasites. the interaction between l. pneumophila and the macrophage exhibits many features found in a wide variety of prokaryotic and eukaryotic intracellular human pathogens. for example, binding to comp ... | 1989 | 2696860 |
| evaluation of phagolysosome fusion in acridine orange stained macrophages infected with histoplasma capsulatum. | a phagosome-lysosome (pl) fusion was performed in vitro using peritoneal cells from normal balb/c mice and the j774.2 macrophage cell line infected with the yeast phase of the fungus histoplasma capsulatum at ratios of 5 x 10(5), 5 x 10(6) or 1 x 10(7) yeasts per 1 x 10(6) macrophages, and phagocytosis was allowed to proceed for 5, 30 and 60 min. macrophage lysosomes were pre-labelled with acridine orange and the cells were challenged with the parasite. fusion was evaluated by fluorescence micro ... | 1989 | 2702784 |
| electrophoretic analysis of histoplasma capsulatum chromosomal dna. | seven chromosome-sized dna molecules in the downs strain of histoplasma capsulatum were resolved by using chromosome-specific dna probes in blot hybridizations of contour-clamped homogeneous electric field (chef) and field-inversion gel electrophoresis (fige) agarose gels. the sizes of the chromosomal dna bands extended from that of the largest saccharomyces cerevisiae chromosome to beyond that of the schizosaccharomyces pombe chromosomes. under our experimental conditions, the order of the five ... | 1989 | 2725508 |
| case 49-1988: histoplasma capsulatum or blastomyces dermatitidis? | | 1989 | 2725627 |
| disseminated histoplasmosis. typical presentation with involvement of the adrenal glands. | a 67-yr-old indonesian patient with disseminated histoplasmosis is described. he had general malaise and fever for 6 months; an oral ulcer, bilateral adrenal gland enlargement and partial adrenal insufficiency were found. an adrenal aspirate contained histoplasma capsulatum. the literature on adrenal involvement in disseminated histoplasmosis is reviewed and it is concluded that bilateral enlargement, demonstrated by sonography or computed tomography, in a patient with general malaise is an impo ... | 1989 | 2725799 |
| disseminated histoplasmosis diagnosed by fine needle aspiration biopsy of the adrenal gland. a case report. | a case of disseminated histoplasmosis diagnosed by fine needle aspiration (fna) biopsy of the adrenal gland is reported for a 60-year-old man who presented with a 40-pound weight loss and abdominal computed tomography showing bilateral adrenal enlargement. fna biopsy of the adrenal gland revealed clusters of macrophages with abundant cytoplasm containing the yeast forms of histoplasma capsulatum. this case emphasizes that fna is effective in diagnosing infectious as well as neoplastic conditions ... | 1989 | 2728788 |
| diagnosis of disseminated histoplasmosis by fine needle aspiration of the adrenal gland. | the cytologic findings in a case of disseminated histoplasmosis diagnosed by fine needle aspiration (fna) of the adrenal gland are presented. bilateral adrenal enlargement was discovered on ct scan in a patient whose systemic symptoms had been attributed to tuberculosis, but were resistant to antituberculous chemotherapy. the aspirate contained numerous histiocytes containing intracytoplasmic organisms with the morphologic features of histoplasma capsulatum. the case shows that radiographically ... | 1989 | 2728789 |
| progressive disseminated histoplasmosis in acquired immune deficiency syndrome: presentation as a steroid-responsive dermatosis. | we describe a patient with acquired immune deficiency syndrome (aids) and disseminated histoplasmosis. he presented with a mild inflammatory dermatitis that improved with topical steroid treatment but was proven to be cutaneous histoplasmosis. the clinical presentations, diagnosis, histopathologic basis, and treatment of disseminated histoplasma capsulatum in patients with aids are discussed. | 1989 | 2743806 |
| requirements for histoplasmin presentation by accessory cells to a histoplasma capsulatum-reactive t-cell line. | we examined the pathways involved in presentation of native histoplasmin by adherent splenocytes (as a source of accessory cells) to jc1, a histoplasma capsulatum-reactive murine t-cell line that is cd4+. jc1 did not respond to accessory cells that had been fixed with paraformaldehyde and then exposed to histoplasmin but did proliferate to antigen-pulsed cells that were subsequently fixed. accessory cells that were coincubated with histoplasmin and sodium azide or 2-deoxy-d-glucose failed to ind ... | 1989 | 2783724 |
| correlative studies of blastogenic responses and interleukin 1 production by mononuclear cells from patients with zoopathogenic fungal infections. | the production of interleukin 1 (il 1) by adherent peripheral blood mononuclear cells (pbmc) was quantitated in 16 individuals infected with histoplasma capsulatum or blastomyces dermatitidis and 16 age-matched controls. in parallel, we measured blastogenic responses to phytohemagglutinin (pha) by pbmc from patients and controls. of the 16 patients, six had pulmonary histoplasmosis, six had disseminated histoplasmosis, two had pulmonary blastomycosis, and two had disseminated blastomycosis. at t ... | 1989 | 2789278 |
| the pathology of fungal infection and colonization in patients with cystic fibrosis. | we used methenamine silver stains to retrospectively evaluate the prevalence of fungi and their associated inflammatory reactions in 63 patients with cystic fibrosis (cf) autopsied between 1982 and 1987. fungi were detected in 13 patients (21%) who fell into three groups: respiratory tract colonization (five patients); localized infection (five patients); and disseminated infection (three patients). hyphae consistent with aspergillus sp were present in five patients; yeast-like cells and/or pseu ... | 1989 | 2793162 |
| comparison of sandwich solid-phase radioimmunoassay and two enzyme-linked immunosorbent assays for detection of histoplasma capsulatum polysaccharide antigen. | detection of a histoplasma capsulatum polysaccharide antigen (hpa) has proved a useful approach to diagnosis of histoplasmosis. two sandwich enzyme-linked immunosorbent assays (elisas) using alkaline phosphatase (ap)-or horseradish peroxidase (hrp)-conjugated antibodies were compared with solid-phase radioimmunoassay (ria) for detection of hpa. the ap-elisa and hrp-elisa were each positive in 17 (89.5%) of 19 urine specimens from patients with disseminated histoplasmosis, while the ria was posit ... | 1989 | 2794560 |
| immunogold electron microscopical detection of tubulin and actin within mycelial phase histoplasma capsulatum capsulatum and h. capsulatum duboisii. | immunogold electron microscopy using lr gold as a resin was undertaken to determine the distribution of actin and tubulin within the hyphae of histoplasma capsulatum capsulatum and h. capsulatum duboisii. both of these proteins were found throughout the cytoplasm when probed with the appropriate monoclonal antibodies. | 1989 | 2797056 |
| histoplasma capsulatum polysaccharide antigen detection in diagnosis and management of disseminated histoplasmosis in patients with acquired immunodeficiency syndrome. | disseminated histoplasmosis is a serious and often rapidly progressive, opportunistic infection in patients with acquired immunodeficiency syndrome (aids), supporting the importance of rapid diagnostic tests. we investigated histoplasma capsulatum polysaccharide antigen (hpa) detection, a promising new method for rapid diagnosis of histoplasmosis. | 1989 | 2801730 |
| [disseminated histoplasmosis after a tropical visit in a patient with known sarcoidosis]. | a 40-year-old man with grade ii sarcoidosis went on a two-week visit to ecuador. afterwards he was treated with low-dose corticosteroids because of slight deterioration on the x-ray film. unilateral hilar enlargement followed by bouts of high fever and a greatly increased erythrocyte sedimentation rate, suggested an acute exacerbation of the sarcoidosis and was, therefore, treated with an increase in steroid dosage plus azathioprine. bronchoalveolar lavage fluid and tissue samples from lung, bon ... | 1989 | 2806107 |
| [current role of deep mycoses in infectious pathology]. | deep mycoses present new aspects characterized by deep, visceral mycotic localisations and septicemia, particularly in immunocompromised conditions. in immunodepressed patients (leukaemia, transplantation), the granulopenia descending to 500 elements/ml leads not only to invasive aspergillosis and candidosis but also to infections due to opportunistic fungi exceptionally or never seen formerly. aids favours opportunistic fungi related to defective cellular immunity as cryptococcus neoformans, re ... | 1989 | 2814246 |
| sarcoidlike manifestations of histoplasmosis. | we have evaluated 11 patients with sarcoidosis accompanied by laboratory evidence for histoplasmosis. clinical findings were typical of those described in sarcoidosis. eight patients were treated with corticosteroids and responded promptly without progression of histoplasmosis. one patient received a 35 mg/kg course of amphotericin b without clinical improvement, but responded appropriately to corticosteroid therapy. another patient had positive sputum cultures for histoplasma capsulatum 5 years ... | 1989 | 2818105 |
| comparison of fluconazole and amphotericin b in treating histoplasmosis in immunosuppressed mice. | fluconazole (uk-49,858) was compared with amphotericin b in treating histoplasmosis in female akr mice immunosuppressed with either cyclophosphamide or cortisone. both drugs protected animals from a lethal challenge with histoplasma capsulatum, but neither regimen resulted in cures since viable organisms were cultured from spleens of survivors. | 1987 | 2830843 |
| heat shock 70 gene is differentially expressed in histoplasma capsulatum strains with different levels of thermotolerance and pathogenicity. | the response to heat shock has been examined in two strains of the dimorphic pathogenic fungus histoplasma capsulatum, which differ considerably in thermotolerance and pathogenicity. the gene for the 70 kd heat shock protein (hsp70) was isolated using a drosophila hsp70 gene to screen a cosmid library of the dna from the temperature-sensitive downs strain (low level of thermotolerance for mice). using the cloned gene as a probe, we have measured the transcription of the endogenous hsp70 gene at ... | 1987 | 2835579 |
| the pathology of aids. | the acquired immunodeficiency syndrome (aids) is a devastating new disease caused by the human immunodeficiency virus (hiv). this retrovirus causes profound immunoincompetence in its infected hosts, who are thereafter susceptible to develop myriad severe and relapsing protozoal, fungal, bacterial, viral, and arthropodal opportunistic infections, as well as unusual malignancies. the more than 50,000 patients who have developed aids in the united states have produced a sudden unexpected deluge of ... | 1988 | 2836878 |
| chronic pulmonary histoplasmosis with an oral lesion. | histoplasmosis is a fungal disease resulting from inhalation of airborne spores of the organism histoplasma capsulatum. the disease is endemic to the ohio and mississippi river valleys. oral lesions are not common, but when present, they are usually associated with the severe disseminated form of histoplasmosis. this particular case is presented as an example of chronic pulmonary histoplasmosis in which a localized gingival lesion represented the initial clinical manifestation of the disease. di ... | 1989 | 2911447 |
| granulomatous mastitis caused by histoplasma and mimicking inflammatory breast carcinoma. | two cases of a lobular, necrotizing granulomatous process causing a unilateral painful breast mass mimicking carcinoma are presented for comparison. while the morphologic appearance in each case was that of lobular granulomatous mastitis, the etiologic agent in one case appeared to be histoplasma capsulatum, based on grocott methenamine silver staining, and represents the second reported case of histoplasmosis involving only breast parenchyma. awareness of the rare entity, granulomatous mastitis ... | 1989 | 2912873 |
| effects of histoplasma capsulatum on murine macrophage functions: inhibition of macrophage priming, oxidative burst, and antifungal activities. | histoplasma capsulatum yeast cells fail to trigger an oxidative burst response in normal murine macrophages. the results of this study, in which an in vitro assay of macrophage antifungal effects was used, extend these findings. during 18 h of incubation, unprimed elicited murine macrophages inhibited h. capsulatum growth only when macrophages were present in great excess. gamma interferon (ifn-gamma)-primed macrophages showed enhanced fungal growth inhibition but a similar requirement for an ex ... | 1989 | 2912897 |
| significance of histoplasma antigen in the cerebrospinal fluid of patients with meningitis. | a radioimmunoassay was previously developed for detection of histoplasma capsulatum antigen in the blood and urine of patients with disseminated histoplasmosis. in this investigation, cerebrospinal fluid (csf) specimens from 14 episodes of histoplasma meningitis occurring in 12 patients were tested by radioimmunoassay. histoplasma capsulatum antigen was detected in the csf of five patients. cerebrospinal fluid cultures were positive for h capsulatum in three of these five patients. antibodies to ... | 1989 | 2916875 |
| production and characterization of murine monoclonal antibodies to histoplasma capsulatum yeast cell antigens. | four monoclonal antibodies (mabs) were produced by immunizing mice with a disrupted yeast cell homogenate of histoplasma capsulatum. mabs 1 and 2 reacted only with the yeast cell antigens of h. capsulatum and blastomyces dermatitidis, whereas mabs 3 and 4 showed broader cross-reactivity. mab 3 cross-reacted with b. dermatitidis, paracoccidioides brasiliensis, sporothrix schenckii, and candida albicans, and mab 4 cross-reacted with b. dermatitidis, c. albicans, coccidioides immitis, aspergillus f ... | 1989 | 2917790 |
| interactions between clay minerals and siderophores affect the respiration of histoplasma capsulatum. | the reduction in the respiration of histoplasma capsulatum in broth culture caused by montmorillonite appeared to be the result, in part, of the interference by the clay with the iron nutrition of the fungus. this interference was apparently the result of the adsorption by the clay of the iron-transporting siderophore (deferricoprogen b) produced by the fungus, as the reduction in respiration was partially alleviated by the addition of foreign siderophores. neither kaolinite nor attapulgite (pal ... | 1986 | 2937365 |
| active substance of histoplasma capsulatum that inhibits blastogenic response of lymphocytes. | a substance inhibiting blast transformation of murine spleen lymphocytes stimulated with various mitogens, such as lps, pha, and pwm, was obtained from yeast-form cells of histoplasma capsulatum. this active substance was partially purified from the cell-free extract by deae-sepharose cl-6b column chromatography. as a result of this partial purification, the inhibitory activity was 1.26 micrograms/ml in terms of id50. materials from h. capsulatum also inhibited blast transformation of murine spl ... | 1986 | 2939323 |
| role of l3t4+ t cells in host defense against histoplasma capsulatum. | cell-mediated immunity is critical in host resistance against the pathogenic fungus histoplasma capsulatum. to explore the role of l3t4+ t cells in protection of mice against h. capsulatum infection, we examined the effect of in vivo treatment with anti-l3t4 monoclonal antibody (mab) gk1.5 on the course of murine disseminated histoplasmosis. treatment with anti-l3t4 antibody caused a profound and selective depletion of l3t4+ t cells that was associated with a significant increase in the number o ... | 1988 | 2968320 |
| impairment of granulomatous inflammatory response to histoplasma capsulatum by inhibitors of angiotensin-converting enzyme. | systemic infection with histoplasma capsulatum induced a granulomatous inflammatory response in the lymphoreticular organs of c57bl/6 mice that was associated with elevated levels of angiotensin-converting enzyme (ace) in the spleens. to determine the influence of ace on the granulomatous response, either captopril or mk 421, two inhibitors of ace, were administered intraperitoneally to mice 6 h after intravenous injection of h. capsulatum and then daily for 1 week. each ace inhibitor sharply re ... | 1985 | 2985505 |
| bronchoalveolar lavage and transbronchial biopsy for the diagnosis of pulmonary infections in the acquired immunodeficiency syndrome. | the efficacy of bronchoalveolar lavage and transbronchial biopsy in diagnosing lung infection was determined in 276 fiberoptic bronchoscopic examinations done on 171 patients with known or suspected acquired immunodeficiency syndrome. of 173 pathogens (pneumocystis carinii, cytomegalovirus, mycobacterium avium-intracellulare, cryptococcus neoformans, m. tuberculosis, coccidioides immitis, and histoplasma capsulatum) identified during the initial evaluation or in the subsequent month, the initial ... | 1985 | 2986505 |
| in-vitro studies with sf 86-327, a new orally active allylamine derivative. | sf 86-327 (sandoz forschungsinstitut) is an orally active allylamine derivative related to naftifine. the antifungal activity of sf 86-327 was compared in vitro with those of naftifine, ketoconazole, and itraconazole (r 51,211, janssen pharmaceutica) by agar dilution. 120 fungal isolates were tested. also, the antifungal activities of sf 86-327 and naftifine against 18 dimorphic pathogens were assayed in vitro by broth dilution. results of these studies support claims that sf 86-327 is a broad s ... | 1985 | 2990057 |
| irreversible block of the mycelial-to-yeast phase transition of histoplasma capsulatum. | p-chloromercuriphenylsulfonic acid (pcms), a sulfhydryl inhibitor, prevented the mycelial-to-yeast transition of the dimorphic fungal pathogen, histoplasma capsulatum. the effect of pcms was specific for the mycelial-to-yeast transformation; it had no effect on growth of either the yeast or mycelial forms or on the yeast-to-mycelial transition. the failure of pcms-treated mycelia to transform to yeast was permanent and irreversible. pcms-treated mycelia could not infect mice but could stimulate ... | 1986 | 3001938 |
| classification of histoplasma capsulatum isolates by restriction fragment polymorphisms. | twenty isolates of the dimorphic, pathogenic fungus histoplasma capsulatum were divided into three classes based on comparisons of restriction enzyme digests of their mitochondrial dna and rdna. the majority of isolates, including most north american strains and the african h. capsulatum var. duboisii variants, belong to class 2. isolates from central america and south america make up class 3. the attenuated downs strain is the only member of class 1. | 1986 | 3005239 |
| comparison of the in vitro and in vivo activity of the bis-triazole derivative uk 49,858 with that of amphotericin b against histoplasma capsulatum. | the antifungal activity of uk 49,858, a difluorophenyl bis-triazole derivative, was evaluated in vitro against seven strains of histoplasma capsulatum and in vivo in akr and c57bl/6 murine models of histoplasmosis. uk 49,858 had a lower toxicity for akr and c57bl/6 mice than amphotericin b did. the therapeutic index of uk 49,858 was 4.3 for akr mice and 7.1 for c57bl/6; with amphotericin b it was 2 for both mouse strains. given orally, uk 49,858 compared favorably with amphotericin b given intra ... | 1986 | 3010852 |
| [disseminated histoplasmosis caused by histoplasma capsulatum and sarcoidosis. apropos of a case]. | histoplasma capsulatum can, sometimes, simulate sarcoidosis, clinically and pathologically. a case is reported in which disseminated histoplasmosis is proved only by fungus granulomatous tissue. another similar cases of the literature incite to propose the practice of special stains, above all gomori-grocott technical, before sarcoid-like lesions. lesions pathogeny was discussed. in our case it's not possible to eliminate the role of primary sarcoidosis in disseminated histoplasmosis. | 1986 | 3013227 |
| monoclonal antibodies against the m-protein and carbohydrate antigens of histoplasmin characterized by the enzyme-linked immunoelectrotransfer blot method. | monoclonal antibodies (mabs) of two different specificities were produced by immunizing mice with the semipurified m antigen of histoplasmin. one type, from clone cb4, was an immunoglobulin m that precipitated a polysaccharide present in histoplasmin and also formed immunoprecipitates with a cross-reactive polysaccharide present in extracts of blastomyces dermatitidis and coccidioides immitis. the second type of mab, from clone ec2, was an immunoglobulin g that reacted in the enzyme-linked immun ... | 1986 | 3017863 |
| treatment of murine histoplasmosis with uk 49,858 (fluconazole). | fluconazole was compared with ketoconazole and with amphotericin b in treatment of pulmonary histoplasmosis in immunologically intact mice and in congenitally athymic nude mice. both fluconazole and amphotericin b increased survival and reduced fungal burden in normal mice. all 3 drugs equally prolonged survival of nude mice challenged with histoplasma capsulatum, and all effectively reduced the fungal burden. fluconazole may be a useful antifungal drug in treatment of murine histoplasmosis. | 1986 | 3021035 |
| histoplasma capsulatum fails to trigger release of superoxide from macrophages. | the yeast form of the dimorphic fungus histoplasma capsulatum survives within macrophages after phagocytosis. to do so, it must avoid, inhibit, or resist a variety of toxic oxygen metabolites. using ferricytochrome c reduction to assay superoxide release, we examined the response of mouse macrophages to the yeast form of various h. capsulatum strains. doses of zymosan as low as 20 particles per macrophage elicited superoxide, whereas h. capsulatum failed to induce superoxide even at 160 yeast ce ... | 1987 | 3025100 |
| modulation of the macrophage oxidative burst by histoplasma capsulatum. | the production of reactive oxygen species by phagocytic cells is an important host defense against invading microorganisms. because pathogens that achieve intracellular survival escape destruction by reactive oxidants, we investigated the relationship between the intracellular survival of h. capsulatum and the macrophage oxidative burst. h. capsulatum yeast failed to stimulate the release of reactive oxygen metabolites in unprimed murine macrophages despite extensive phagocytosis of the microorg ... | 1987 | 3025301 |
| role of the adherence-promoting receptors, cr3, lfa-1, and p150,95, in binding of histoplasma capsulatum by human macrophages. | the principal host cell of h. capsulatum (hc) is the m phi within which the pathogenic yeast phase of the fungus multiplies during active disease. the initial interaction between hc yeasts and m phi therefore is a crucial step in the pathogenesis of histoplasmosis. in the present study, we have identified the major receptor mechanism that mediates the attachment of unopsonized hc yeasts to human monocyte-derived m phi from peripheral blood. binding of hc yeasts by m phi is rapid, temperature dep ... | 1987 | 3025331 |
| early experience with itraconazole in vitro and in patients: pharmacokinetic studies and clinical results. | the efficacy of itraconazole, a new triazole antifungal agent, was studied in vitro and assessed in patients. the mics of itraconazole for 16 strains of aspergillus fumigatus were in the same range as those of amphotericin b: less than 0.09-0.36 microgram/ml vs. less than 0.09-0.78 microgram/ml, respectively. eight adult patients with systemic fungal infections were treated orally with 100-200 mg of itraconazole two times a day. a patient with relapsing histoplasmosis (histoplasma capsulatum var ... | 1987 | 3027850 |
| morphogenesis and pathogenicity of histoplasma capsulatum. | the sulfhydryl blocking agent p-chloromercuriphenylsulfonic acid (pcms) irreversibly inhibited the mycelium-to-yeast transitions of two virulent strains of histoplasma capsulatum, g184a and g222b, when the temperature of incubation was raised to 37 degrees c, and the block persisted even after the cultures were washed free of pcms. instead of transforming to yeast cells, pcms-treated mycelia continued to grow as mycelia at the elevated temperatures. a less virulent strain (downs) was more temper ... | 1987 | 3032799 |
| fungistatic and fungicidal effects of amphotericin b, ketoconazole and fluconazole (uk 49,858) against histoplasma capsulatum in vitro and in vivo. | | 1987 | 3035371 |
| mediastinal fibrosis complicating histoplasmosis. | mediastinal fibrosis, the most serious late complication of remote infection by histoplasma capsulatum, is a thick, dense fibrotic capsule which surrounds a small mediastinal focus of old caseous adenitis. the fibrotic process may accrue over prolonged periods and extend within the lumina of critical mediastinal structures to produce complete occlusion. we summarized clinical and radiographic data for 71 patients with mediastinal fibrosis; the criteria for inclusion were the clinical demonstrati ... | 1988 | 3045478 |
| caecal perforation in a renal transplant patient with disseminated histoplasmosis. | a renal transplant patient developed a fatal caecal perforation after histoplasma capsulatum infection acquired abroad. disseminated histoplasmosis is an uncommon fungal infection, usually seen in patients with impaired immunity. the diagnosis should be considered in immunosuppressed patients who develop prolonged fever or whose health deteriorates unexpectedly after travelling overseas. the infection is endemic in parts of the united states of america but occurs all over the world. rapid diagno ... | 1988 | 3056989 |
| in vitro interactions between blastomyces dermatitidis and other zoopathogenic fungi. | the results of in vitro interactions between colonies of blastomyces dermatitidis and six other zoopathogenic fungi are reported. the interactions were found to range from neutral with histoplasma capsulatum and candida albicans to strongly antagonistic with microsporum gypseum, pseudallescheria boydii, and sporothrix schenckii, and including lysis by cryptococcus neoformans. these observations suggest that interactions between zoopathogenic fungi may be one of the biotic factors likely to influ ... | 1988 | 3058276 |
| fungal infections in aids. histoplasmosis and coccidioidomycosis. | histoplasma capsulatum and coccidioides immitis are two fungi that are regional in occurrence and cause opportunistic fungal infections in patients with aids. many cases of histoplasmosis have been reported in patients months or years after they have been in an endemic area. these are obviously cases of reactivation of latent infections. with coccidioidomycosis, the cases have been reported from endemic areas, but some also appear to be reactivation infections, and we should anticipate such case ... | 1988 | 3060528 |
| fungal infections in aids. cryptococcosis. | cryptococcus neoformans, histoplasma capsulatum, and coccidioides immitis are the three fungi that regularly cause disseminated, life-threatening disease in patients with aids. cryptococcosis is the fourth most common opportunistic infection in patients with aids and results in meningitis or pneumonia or both, in most cases. in addition, there have been unusual focal infections described and even unexplained fever alone. in any patient at risk for hiv infection, routine screening tests should in ... | 1988 | 3060529 |
| systemic fungal infections: diagnosis and treatment. i. sporotrichosis. | sporotrichosis is a fungal infection caused by the dimorphic fungus sporothrix schenckii. although this fungal microorganism is labeled a true pathogen, its virulence is less than that of other fungal pathogens, such as coccidioides immitis, histoplasma capsulatum, and blastomyces dermatitidis. this article discusses its clinical manifestations, experimental models, diagnosis, and therapy. | 1988 | 3062093 |
| an autopsy case of disseminated histoplasmosis probably due to infection from a renal allograft. | an autopsy case of a 52-year-old japanese male, who died of disseminated histoplasmosis, is reported. he had received a cadaveric renal allograft 4 years prior to death. the donor was a 33-year-old american negro male, who had resided in texas. the patient had been treated with immunosuppressive drugs after renal transplantation, and mycotic pneumonia developed 3 months before death. at autopsy, acute necrotizing lesions composed of histiocytes were observed in the transplanted kidney, lungs, pr ... | 1988 | 3064545 |
| [isolation of histoplasma capsulatum in bats in cuba]. | | 1988 | 3072612 |
| grouping of aspergillus species with exoantigens. | ninety-two slant extracts prepared from 2-week-old cultures of seven aspergillus groups, nonsporulating "albino-type" a.fumigatus, blastomyces dermatitidis, histoplasma capsulatum, 3 penicillium spp., 2 pseudallescheria spp., 3 paecilomyces spp., and acremonium sp., were analyzed concurrently against antisera to a. fumigatus, a. flavus, a. nidulans, a. niger, and a. terreus. the extract of each of the aforementioned five pathogenic aspergillus spp. produced 2-11 specific antigen-antibody complex ... | 1986 | 3086014 |
| inhibition of the intracellular growth of histoplasma capsulatum by recombinant murine gamma interferon. | recombinant murine gamma interferon as well as lymphokines prepared from immune splenocytes and concanavalin a-stimulated t-cell hybridoma activated normal mouse peritoneal macrophages to inhibit the intracellular growth of histoplasma capsulatum. the activities of the lymphokine from immune splenocytes and of recombinant murine gamma interferon were neutralized by rabbit anti-murine gamma interferon antibody. the intracellular yeasts were not killed by the interaction even though growth was com ... | 1987 | 3104206 |
| immunopathology of acute experimental histoplasmic choroiditis in the primate. | the immunopathologic features of experimental acute histoplasmic choroiditis were studied in the nonhuman primate. using an indirect immunoperoxidase technique, a panel of hybridoma-derived anti-human monoclonal antibodies, recognizing distinct lymphoid cell and macrophage surface antigens, have been adapted for use in the primate system. twenty-two individual foci of histoplasmic choroiditis from five eyes were studied at time periods from 20 to 60 days post intracarotid injection of yeast phas ... | 1987 | 3110092 |
| evaluation of enzyme immunoassay as a rapid screening test for histoplasmosis and blastomycosis. | this study evaluates the usefulness of enzyme immunoassay (eia) as a screening test for serum antibodies to histoplasma capsulatum and blastomycoses dermatitidis and compares the results using this assay with those using complement fixation (cf), immunodiffusion (id), and radioimmunoassay (ria), in 12 patients with active histoplasmosis, 12 with active blastomycosis, 28 with other acute or chronic lung diseases and 25 healthy blood donors from an endemic area; eia was as sensitive as ria and mor ... | 1987 | 3113304 |
| immunofluorescence as an adjunct to the histopathologic diagnosis of chagas' disease. | we have developed a new indirect immunofluorescence procedure for identifying trypanosoma cruzi amastigotes in sections of formalin-fixed, paraffin-embedded tissue pretreated with a 0.25% trypsin solution to enhance immunofluorescence. in sections of human and mouse myocardia infected with t. cruzi and stained by this procedure, both intact amastigotes and phagocytosed amorphous antigen were intensely fluorescent and easily detected; nonspecific background fluorescence was absent or minimal. no ... | 1988 | 3128582 |