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isolation and characterization of bacteriocinogenic lactic bacteria from m-tuba and tepache, two traditional fermented beverages in méxico.mexican tuba (m-tuba) and tepache are mexican fermented beverages prepared mainly with pineapple pulp and coconut palm, respectively. at present, reports on the microbiota and nutritional effects of both beverages are lacking. the purpose of this study was to determine whether m-tuba and tepache contain cultivable lactic acid bacteria (lab) capable of producing bacteriocins. tepache and m-tuba contain mesophilic aerobic bacteria, lab, and yeast. bacillus subtilis, listeria monocytogenes, listeri ...201526405529
antibacterial activity of selected malaysian honey.antibacterial activity of honey is mainly dependent on a combination of its peroxide activity and non-peroxide components. this study aims to investigate antibacterial activity of five varieties of malaysian honey (three monofloral; acacia, gelam and pineapple, and two polyfloral; kelulut and tualang) against staphylococcus aureus, bacillus cereus, escherichia coli, and pseudomonas aeruginosa.201323758747
applications of microencapsulated bifidobacterium longum with eleutherine americana in fresh milk tofu and pineapple juice.bifidobacterium longum was microencapsulated by extrusion technique and added in fresh milk tofu and pineapple juice. microencapsulation of b. longum with eleutherine americana extract, oligosaccharides extract, and commercial fructo-oligosaccharides was assessed for the bacterial survival after sequential exposure to simulated gastric and intestinal juices, and refrigeration storage. microencapsulated b. longum with the extract and oligosaccharides extract in the food products showed better sur ...201525854832
investigation of the factors influencing the survival of bifidobacterium longum in model acidic solutions and fruit juices.the survival of bifidobacterium longum ncimb 8809 was studied during refrigerated storage for 6weeks in model solutions, based on which a mathematical model was constructed describing cell survival as a function of ph, citric acid, protein and dietary fibre. a central composite design (ccd) was developed studying the influence of four factors at three levels, i.e., ph (3.2-4), citric acid (2-15g/l), protein (0-10g/l), and dietary fibre (0-8g/l). in total, 31 experimental runs were carried out. a ...201125212334
functional properties of a cysteine proteinase from pineapple fruit with improved resistance to fungal pathogens in arabidopsis thaliana.in plant cells, many cysteine proteinases (cps) are synthesized as precursors in the endoplasmic reticulum, and then are subject to post-translational modifications to form the active mature proteinases. they participate in various cellular and physiological functions. here, accp2, a cp from pineapple fruit (ananas comosus l.) belonging to the c1a subfamily is analyzed based on the molecular modeling and homology alignment. transcripts of accp2 can be detected in the different parts of fruits (p ...201424566309
antibacterial mode of action of violacein from chromobacterium violaceum utm5 against staphylococcus aureus and methicillin-resistant staphylococcus aureus (mrsa).violacein, violet pigment produced by chromobacterium violaceum, has attracted much attention recently due to its pharmacological properties including antibacterial activity. the present study investigated possible antibacterial mode of action of violacein from c. violaceum utm5 against staphylococcus aureus and methicillin-resistant s. aureus (mrsa) strains. violet fraction was obtained by cultivating c. violaceum utm5 in liquid pineapple waste medium, extracted, and fractionated using ethyl ac ...201728361404
production and characterization of violacein by locally isolated chromobacterium violaceum grown in agricultural wastes.the present work highlighted the production of violacein by the locally isolated chromobacterium violaceum (genbank accession no. hm132057) in various agricultural waste materials (sugarcane bagasse, solid pineapple waste, molasses, brown sugar), as an alternative to the conventional rich medium. the highest yield for pigment production (0.82 g l⁻¹) was obtained using free cells when grown in 3 g of sugarcane bagasse supplemented with 10% (v/v) of l-tryptophan. a much lower yield (0.15 g l⁻¹) wa ...201222278051
citrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis a and b syndromes.citrus psorosis virus (cpsv) is the causal agent of psorosis, a serious and widespread citrus disease. two syndromes of psorosis, psa and psb, have been described. psb is the most aggressive and rampant form. previously, we obtained pineapple sweet orange plants transformed with a hairpin construct derived from the cpsv coat protein gene (ihpcp). some of these plants were resistant to cpsv 90-1-1, a psa isolate homologous to the transgene. in this study, we found that expression of the ihpcp tra ...201727891561
antimicrobial activity and potential use of monoterpenes as tropical fruits preservatives.banana, papaya and pineapple are the most consumed tropical fruits in the world, being brazil one of the main producers. fungi colletotrichum musae, colletotrichum gloeosporioides and fusarium subglutinans f.sp. ananas cause severe post harvest diseases and losses in fruits quality. the aim of this work was to evaluate the effectiveness of five monoterpenes to inhibit the mycelial growth and conidia germination of these three phytopathogens. the monoterpenes citral, citronellal, l-carvone, isopu ...200824031197
decolorization of palm oil mill effluent using growing cultures of curvularia clavata.agricultural wastewater that produces color are of environmental and health concern as colored effluent can produce toxic and carcinogenic by-products. from this study, batch culture optimization using response surface methods indicated that the fungus isolated from the pineapple solid waste, curvularia clavata was able to decolorize sterile palm oil mill effluent (pome) which is mainly associated with polyphenol and lignin. results showed successful decolorization of pome up to 80 % (initial ad ...201424327114
phylogeny and classification of dickeya based on multilocus sequence analysis.bacterial heart rot of pineapple reported in hawaii in 2003 and reoccurring in 2006 was caused by an undetermined species of dickeya. classification of the bacterial strains isolated from infected pineapple to one of the recognized dickeya species and their phylogenetic relationships with dickeya were determined by a multilocus sequence analysis (mlsa), based on the partial gene sequences of dnaa, dnaj, dnax, gyrb and recn. individual and concatenated gene phylogenies revealed that the strains f ...201324003072
characteristics of lactic acid bacteria isolates and their effect on silage fermentation of fruit residues.the natural lactic acid bacteria (lab) population, chemical composition, and silage fermentation of fruit residues were studied. eighty-two strains of lab were isolated from fruit residues such as banana leaf and stem, pineapple peel, and papaya peel. all strains were gram-positive and catalase-negative bacteria, and they were divided into 7 groups (a-g) according to morphological and biochemical characters. strains in groups a to f were rods, and group g was cocci. group f produced gas from glu ...201627108171
isolation of saccharomyces cerevisiae from pineapple and orange and study of metal's effectiveness on ethanol production.in view of the anticipated shortage of the traditional supplies of fossil fuels, there is a great deal of interest in the production of ethanol as an alternative biofuel in recent years. the main objective of this research work was to isolate and characterize stress tolerant, high potential ethanol producing yeast strains from various fruit peel. two yeast isolates from pineapple (pa) and orange (or) have been isolated, characterized on the basis of morphological and physic-chemical characters a ...201728386473
the efficacy of mentha arvensis l. and m. piperita l. essential oils in reducing pathogenic bacteria and maintaining quality characteristics in cashew, guava, mango, and pineapple juices.this study evaluated the ability of the essential oil from mentha arvensis l. (maeo) and m. piperita l. (mpeo) to induce ≥5-log reductions in counts (cfu/ml) of e. coli, l. monocytogenes, and salmonella enterica serovar enteritidis in brain-heart infusion broth (bhib) and cashew, guava, mango, and pineapple juices during refrigerated storage (4±0.5°c). the effects of the incorporation of these essential oils on some physicochemical and sensory parameters of juices were also evaluated. the incorp ...201627648760
survival and expression of acid resistance genes in shiga toxin-producing escherichia coli acid adapted in pineapple juice and exposed to synthetic gastric fluid.the aim of this research was to determine the ability of non-o157 shiga toxin-producing escherichia coli (stec) serogroups to survive with exposure to synthetic gastric fluid (sgf) after adaptation to pineapple juice (pj) at room and refrigerated temperatures compared to e. coli o157:h7 and to examine the relative transcriptional expression of acid resistance (ar) genes, rpos, gada and adia.201627377411
antibacterial and antiproliferative peptides in synbiotic yogurt-release and stability during refrigerated storage.the search for alternative therapeutics is on the rise due to the extensive increase in bacterial resistance to various conventional antibiotics and side effects of conventional cancer therapies. bioactive peptides released from natural sources such as dairy foods by lactic acid bacteria have received attention as a potential source of biotherapeutic peptides. however, liberation of peptides in yogurt depends on proteolytic activities of the cultures used. thus, this research was conducted to es ...201626995128
phenylpropanoid glycosides from the leaves of ananas comosus.two new phenylpropanoid glycosides, named β-d-(1-o-acetyl-3,6-o-diferuloyl) fructofuranosyl β-d-6'-o-acetylglucopyranoside (1) and β-d-(1-o-acetyl-3,6-o-diferuloyl) fructofuranosyl α-d-glucopyranoside (2), along with two known analogues (3-4) and four glycerides (5-8), were isolated from the etoac extract of the leaves of ananas comosus. their structures were elucidated on the basis of 1d- and 2d-nmr analyses, as well as hr-esi-ms experiments. compounds 1-4 showed significant antibacterial activ ...201526882684
inactivation of escherichia coli, listeria monocytogenes, and salmonella enteritidis by cymbopogon citratus d.c. stapf. essential oil in pineapple juice.in the present study, the efficacy of cymbopogon citratus d.c. stapf. essential oil (cceo) to provoke a 5-log cfu/ml (5-log) inactivation in a mixed composite of escherichia coli, listeria monocytogenes, and salmonella enterica serovar enteritidis in pineapple (ananas comosus (l.) merril) juice (4°c) was assessed. moreover, the effects of cceo on the physicochemical and sensory quality parameters of pineapple juice were evaluated. the mic of cceo was 5 μl/ml against the composite mix examined. f ...201626818981
molecular cloning and characterization of udp-glucose: furaneol glucosyltransferase gene from grapevine cultivar muscat bailey a (vitis labrusca × v. vinifera).2,5-dimethyl-4-hydroxy-3(2h)-furanone (furaneol) is an important aroma compound in fruits, such as pineapple and strawberry, and is reported to contribute to the strawberry-like note in some wines. several grapevine species are used in winemaking, and furaneol is one of the characteristic aroma compounds in wines made from american grape (vitis labrusca) and its hybrid grape. furaneol glucoside was recently isolated as an important furaneol derivative from the hybrid grapevine cultivar, muscat b ...201526160581
dai nippon printing co., ltd medi·ca cc for enumeration of coliform bacteria.a ready-made dry medium method for coliform count, the medi·ca cc method, was compared to the violet red bile agar method (bacteriological analytical manual, chapter 4, enumeration of escherichia coli and the coliform bacteria, section g) for nine raw foods from four food categories: raw ground pork, raw lamb, raw ground chicken, raw tuna fillet, raw salmon fillet, raw shrimp, fresh peeled banana, fresh cut pineapple, and fresh cut apple. the 95% confidence interval for the mean difference betwe ...201525857880
a standardised challenge model with an enterotoxigenic f4+ escherichia coli strain in piglets assessing clinical traits and faecal shedding of fae and est-ii toxin genes.this study evaluated the effect of five feed additives on post weaning diarrhoea (pwd) in piglets challenged 3 d after weaning with an enterotoxigenic escherichia coli strain (etec). in three experimental runs, a total of 84 piglets was weaned at 21 days of age and randomly assigned to seven treatments. as dietary treatment, piglets were fed a basal diet or diets with addition of bovine colostrum (0.2%), pineapple stem extract containing bromelain (0.2%), an autolysed yeast preparation (saccharo ...201425313936
characterization of the third serk gene in pineapple (ananas comosus) and analysis of its expression and autophosphorylation activity in vitro.two somatic embryogenesis receptor-like kinase genes (identified as acserk1 and acserk2) have previously been characterized from pineapple (ananas comosus). in this work, we describe the characterization of a third gene (acserk3) in this family. acserk3 had all the characteristic domains and shared extensive sequence homology with other plant serks. acserk3 expression was studied by in situ hybridization and quantitative real-time pcr to analyze its function. intense in situ hybridization signal ...201425249776
evaluation of antibacterial activities of silver nanoparticles green-synthesized using pineapple leaf (ananas comosus).pineapple leaf was used in this study for the synthesis of silver nanoparticles based on the search for sustainable synthetic means. indeed, this offered an economical and sustainable synthetic route relative to expensive and toxic chemical methods. the leaf extract was used and the corresponding nanoparticles obtained were subjected to uv-vis analysis at different times. the uv-vis was used to monitor the silver nanoparticle formation through sampling at time intervals. the formation of silver ...201424268599
functional characterization of recombinant bromelain of ananas comosus expressed in a prokaryotic system.bromelain (brm) is a defense protein present in the fruit and stem of pineapple (ananas comosus) and it is grouped as a cysteine protease enzyme with diversified medicinal uses. based on its therapeutic applications, bromelain has got sufficient attention in pharmaceutical industries. in the present study, the full coding gene of bromelain in pineapple stem (1,093 bp) was amplified by rt-pcr. the pcr product was cloned, sequenced, and characterized. the sequence analysis of the gene revealed the ...201423921698
the use of near infrared spectroscopy and multivariate techniques to differentiate escherichia coli and salmonella enteritidis inoculated into pulp juice.this study shows the application and usefulness of near infrared (nir) transflectance spectra measurements in the identification and classification of escherichia coli and salmonella enteritidis from commercial fruit pulp (pineapple). principal component analysis (pca), soft independent modeling of class analogy (simca) analysis and partial least-squares discriminant analysis (pls-da) were used in the analysis. it was not possible to obtain total separation between the samples using pca and simc ...201323470963
resonance assignments and secondary structure of a phytocystatin from ananas comosus.a cdna encoding a cysteine protease inhibitor, cystatin was cloned from pineapple (ananas comosus l.) stem. this clone was constructed into an expression vector and expressed in e. coli and purified to homogeneous. the recombinant pineapple cystatins (accys) showed effectively inhibitory activity toward cysteine proteases including papain, bromelain, and cathepsin b. in order to unravel its inhibitory action from structural point of view, multidimensional heteronuclear nmr techniques were used t ...201221814766
bromelain, a cysteine protease from pineapple (ananas comosus) stem, is an inhibitor of fungal plant pathogens.this study aimed to evaluate the effect of bromelain, a cysteine protease isolated from pineapple (ananas comosus), on growth of several agronomically important fungal pathogens.201222537505
fusarium proliferatum strains change fumonisin biosynthesis and accumulation when exposed to host plant extracts.fumonisin concentrations in mycelia and media were studied in liquid fusarium proliferatum cultures supplemented with host plant extracts. furthermore, the kinetics of fumonisin accumulation in media and mycelia collected before and after extract addition was analysed as well as the changes in the expression of the fum1 gene. fumonisin content in culture media increased in almost all f. proliferatum strains shortly after plant extracts were added. the asparagus extract induced the highest fb lev ...201627268248
host extract modulates metabolism and fumonisin biosynthesis by the plant-pathogenic fungus fusarium proliferatum.fusarium proliferatum is a common pathogen able to infect a broad range of agriculturally important crops. recently, some evidence for genetic variance among the species genotypes in relation to their plant origin has been reported. mycotoxin contamination of plant tissues is the most important threat caused by f. proliferatum and fumonisins b (fbs) are the principal mycotoxins synthesized. the toxigenic potential of the pathogen genotypes is variable and also the reaction of different host plan ...201525462926
identification and validation of reference genes to study the gene expression in gluconacetobacter diazotrophicus grown in different carbon sources using rt-qpcr.gluconacetobacter diazotrophicus strain pal5 is a nitrogen-fixing endophytic bacterium originally isolated from sugarcane and later on was found to colonize other plants such as rice, elephant grass, sweet potato, coffee, and pineapple. currently, g. diazotrophicus has been considered a plant growth-promoting bacterium due to its characteristics of biological nitrogen fixation, phytohormone secretion, solubilization of mineral nutrients and antagonism to phytopathogens. reverse transcription fol ...201222814372
in vitro and in vivo evaluation of the activity of pineapple (ananas comosus) on haemonchus contortus in santa inês sheep.the development of resistance to anthelmintics has prompted research into alternative methods of controlling intestinal nematodes in ruminants. this study aimed to assess the activity of ananas comosus on haemonchus contortus in santa inês sheep. the aqueous extract of pineapple skin (aeps), bromelain from pineapple stems (b4882) and residue from pineapple processing was evaluated in in vitro and in vivo tests. the enzymatic activity of substances was analyzed by the azocasein method. the egg ha ...201323688638
in vitro anthelmintic activity of crude extracts of selected medicinal plants against haemonchus contortus from sheep.ethanol extracts of 25 plant species were screened for anthelmintic effects against haemonchus contortus. ethanol extracts of each plant were used at various concentrations (10, 20 and 30%) to treat 10-day faecal cultures, incubated at 27°c with control cultures which were treated with ethanol for 48 h. five plants with high efficacies (ananas comosus, aloe ferox, allium sativum, lespedeza cuneata and warburgia salutaris) were selected from the first screening for further investigation using eth ...201322449714
benefit of supplements in functional dyspepsia after treatment of helicobacter pylori.functional dyspepsia, though benign, leads to deterioration of the quality of life and high costs for healthcare systems. the optimal therapy for functional dyspepsia is still to be defined because of its multifactorial pathogenesis. in an open multicentric study of patients with functional dyspepsia, we prospectively evaluated the benefit of treatment with a food supplement composed of sodium alginate, carbonate calcium, pineapple, papaya, ginger, α-galactosidase and fennel (perdiges, bioten sn ...201425384804
effect of refrigerated storage on probiotic viability and the production and stability of antimutagenic and antioxidant peptides in yogurt supplemented with pineapple peel.fruit by-products are good resources of carbohydrates, proteins, vitamins, and minerals, which may function as growth nutrients for probiotic bacteria. this research aimed at evaluating effects of pineapple peel powder addition on the viability and activity of lactobacillus acidophilus (atcc 4356), lactobacillus casei (atcc393), and lactobacillus paracasei ssp. paracasei (atcc baa52) in yogurts throughout storage at 4°c for 28d. plain and probiotic yogurts supplemented with or without pineapple ...201526142843
sonicated pineapple juice as substrate for l. casei cultivation for probiotic beverage development: process optimisation and product stability.the aim of this study was to evaluate the use of sonicated pineapple juice as substrate for producing a probiotic beverage by lactobacillus casei nrrl b442. maximal microbial viability was found by cultivating l. casei at 31°c and ph 5.8 (optimised conditions). after fermentation, samples of sweetened and non-sweetened juice were stored. after 42 days of storage under refrigeration (4°c), the microbial viability was 6.03 log cfu/ml in the non-sweetened sample and 4.77 log cfu/ml in the sweetened ...201323561104
fresh-cut pineapple as a new carrier of probiotic lactic acid bacteria.due to the increasing interest for healthy foods, the feasibility of using fresh-cut fruits to vehicle probiotic microorganisms is arising scientific interest. with this aim, the survival of probiotic lactic acid bacteria, belonging to lactobacillus plantarum and lactobacillus fermentum species, was monitored on artificially inoculated pineapple pieces throughout storage. the main nutritional, physicochemical, and sensorial parameters of minimally processed pineapples were monitored. finally, pr ...201425093163
genome sequence of lactobacillus paracasei strain lc-ikematsu, isolated from a pineapple in okinawa, japan.the draft genome sequence of lactobacillus paracasei strain lc-ikematsu, isolated from a pineapple in okinawa, was determined. the total length of the 87 contigs was 3.08 mb with a g+c content of 46.2% and 2,946 coding sequences. the genome analysis revealed its biosynthetic ability of 11 amino acids.201728153910
transcriptional reprogramming and phenotypic switching associated with the adaptation of lactobacillus plantarum c2 to plant niches.lactobacillus plantarum has been isolated from a large variety of ecological niches, thus highlighting its remarkable environmental adaptability as a generalist. plant fermentation conditions markedly affect the functional features of l. plantarum strains. we investigated the plant niche-specific traits of l. plantarum through whole-transcriptome and phenotypic microarray profiles. carrot (cj) and pineapple (pj) juices were chosen as model systems, and mrs broth was used as a control. a set of 3 ...201627273017
metabolic responses of lactobacillus plantarum strains during fermentation and storage of vegetable and fruit juices.strains of lactobacillus plantarum were grown and stored in cherry (chj), pineapple (pj), carrot (cj), and tomato (tj) juices to mimic the chemical composition of the respective matrices. wheat flour hydrolysate (wfh), whey milk (w), and mrs broth were also used as representatives of other ecosystems. the growth rates and cell densities of l. plantarum strains during fermentation (24 h at 30°c) and storage (21 days at 4°c) differed only in part, being mainly influenced by the matrix. chj and pj ...201424487533
studies on production of alcoholic beverages from some tropical fruits.the varieties of alcoholic beverages from watermelon; watermelon-banana and watermelon-pineapple mixtures were produced by using monoculture and mixed culture fermentation techniques. three yeast species, namely, saccharomyces cerevisiae, kleochera apicalata, torulospora delbruckii and four bacterial species leuconostoc oenos, lactobacillus sp, micrococcus luteus and streptococcus lactis were identified during the study. the daily succession of these organisms in the various fermenting samples d ...201022815578
identification, stress tolerance, and antioxidant activity of lactic acid bacteria isolated from tropically grown fruits and leaves.from 6 samples of tropically grown fruits and leaves, 10 lactic acid bacteria belonging leuconostoc, weissella, and lactobacillus species were isolated and identified by 16s rrna gene sequencing and (gtg)5 fingerprinting. acidification kinetics determined from bhi broth cultures showed genus-related patterns. in particular, weissella cibaria appeared to act as a potent acidifier. tolerance of isolates to acid, oxidative, or salt stress was highly variable and strain dependent. isolate s14 (leuco ...201627197991
growth potential of listeria monocytogenes and staphylococcus aureus on fresh-cut tropical fruits.the objective of this study was to evaluate the fate of staphylococcus aureus, listeria monocytogenes, and natural microbiota on fresh-cut tropical fruits (pitaya, mango, papaya and pineapple) with commercial pvc film at different storage temperature (5, 13, and 25 °c). the results showed that s. aureus, l. monocytogenes, and natural microbiota increased significantly on fresh-cut tropical fruits at 25 °c. both pathogen and natural microbiota were able to grow on fresh-cut tropical fruits at 13 ...201526862605
characterization of mexican coriander (eryngium foetidum) essential oil and its inactivation of listeria monocytogenes in vitro and during mild thermal pasteurization of pineapple juice.the aim of this work was to characterize the essential oil (eo) of eryngium foetidum (efeo) and assess its activity toward listeria monocytogenes in broth and during thermal inactivation of the pathogen in pineapple juice. in this respect, efeo was chemically characterized, and its antilisteria potential in broth as a function of ph, cell load, and efeo concentration was assessed through a central composite design. furthermore, the inactivation kinetics of l. monocytogenes in the juice were asse ...201424674435
pineappledb: an online pineapple bioinformatics resource.a world first pineapple est sequencing program has been undertaken to investigate genes expressed during non-climacteric fruit ripening and the nematode-plant interaction during root infection. very little is known of how non-climacteric fruit ripening is controlled or of the molecular basis of the nematode-plant interaction. pineappledb was developed to provide the research community with access to a curated bioinformatics resource housing the fruit, root and nematode infected gall expressed se ...200516202174
pineapple (ananas comosus l. merr.) wine production in angola: characterisation of volatile aroma compounds and yeast native flora.a pineapple vinification process was conducted through inoculated and spontaneous fermentation to develop a process suitable for a quality beverage during two successive vintages in huambo, angola. wines obtained with the conventional saccharomyces cerevisiae strain, were analysed by gas chromatography, and a total of 61 volatile constituents were detected in the volatile fraction and 18 as glycosidically bound aroma compounds. concentration levels of carbonyl and sulphur compounds were in agree ...201727783969
phenotypic and molecular diversity of meyerozyma guilliermondii strains isolated from food and other environmental niches, hints for an incipient speciation.meyerozyma guilliermondii is a yeast species widely isolated from several natural environments and from fruit; in medical microbiology it is known as the teleomorph of the opportunistic pathogen candida guilliermondii, which causes about 2% of the human blood infections. this yeast is also promising in a variety of biotechnological applications as vitamins production and post-harvest control. the question if isolates from different sources are physiologically and genetically similar, or if the v ...201525791010
stem bromelain-induced macrophage apoptosis and activation curtail mycobacterium tuberculosis persistence.mycobacterium tuberculosis, the causative agent of tuberculosis, has a remarkable ability to usurp its host's innate immune response, killing millions of infected people annually. one approach to manage infection is prevention through the use of natural agents. in this regard, stem bromelain (sbm), a pharmacologically active member of the sulfhydryl proteolytic enzyme family, obtained from ananas comosus and possessing a remarkable ability to induce the innate and acquired immune systems, is imp ...201222615313
pantoea ananatis utilizes a type vi secretion system for pathogenesis and bacterial competition.type vi secretion systems (t6sss) are a class of macromolecular machines that are recognized as an important virulence mechanism in several gram-negative bacteria. the genome of pantoea ananatis lmg 2665(t), a pathogen of pineapple fruit and onion plants, carries two gene clusters whose predicted products have homology with t6ss-associated gene products from other bacteria. nothing is known regarding the role of these t6ss-1 and t6ss-3 gene clusters in the biology of p. ananatis. here, we presen ...201525411959
a novel low-temperature-active pectin methylesterase from penicillium chrysogenum f46 with high efficiency in fruit firming.a pectin methylesterase gene (pe8f46) was cloned from penicillium chrysogenum f46 and successfully expressed in pichia pastoris. the full-length cdna consists of 969 bp and encodes a 322-residue polypeptide with the calculated molecular weight of 34.1 kda. deduced pe8f46 belongs to family 8 of carbohydrate esterases and shares 54% identity with a functionally characterised counterpart from myceliophthora thermophile. purified recombinant pe8f46 showed the optimal activity at ph 5.0 and 40°c, and ...201424874380
analysis of pineapple mealybug wilt associated virus -1 and -2 for potential rna silencing suppressors and pathogenicity factors.higher plants use rna silencing to defend against viral infections. as a counter defense, plant viruses have evolved proteins that suppress rna silencing. mealybug wilt of pineapple (mwp), an important disease of pineapple, has been associated with at least three distinct viruses, pineapple mealybug wilt associated virus -1, -2, and -3 (pmwav-1, -2, and -3). selected open reading frames (orfs) of pmwav-1 and pmwav-2 were screened for their local and systemic suppressor activities in agrobacteriu ...201525751306
development of an immunomagnetic capture-reverse transcriptase-pcr assay for three pineapple ampeloviruses.a semi-automated, immunomagnetic capture-reverse transcription pcr (imc-rt-pcr) assay for the detection of three pineapple-infecting ampeloviruses, pineapple mealybug wilt-associated virus-1, -2 and -3, is described. the assay was equivalent in sensitivity but more rapid than conventional immunocapture rt-pcr. the assay can be used either as a one- or two-step rt-pcr and allows detection of the viruses separately or together in a triplex assay from fresh, frozen or freeze-dried pineapple leaf ti ...200918996414
closterovirus infection and mealybug exposure are necessary for the development of mealybug wilt of pineapple disease.abstract the roles of pineapple mealybug wilt-associated viruses (pmwavs) and mealybug (dysmicoccus spp.) feeding in the etiology of mealybug wilt of pineapple (mwp) were evaluated. container-grown pineapple (ananas comosus) plants from five commercially grown hawaiian proprietary selections and a field study utilizing a randomized complete block design were used to test four treatments for induction of mwp: pmwav-1-free and pmwav-1-infected plants maintained mealybug-free, and pmwav-1-free and ...200218944017
a highly sensitive single-tube nested pcr assay for the detection of pineapple mealybug wilt associated virus-2 (pmwav-2).an assay was developed for the detection of pineapple mealybug wilt associated virus-2 (pmwav-2), an important factor in the etiology of mealybug wilt of pineapple. the assay combines reverse transcription of rna isolated from pineapple with a specific and very sensitive, single, closed-tube nested polymerase chain reaction (pcr) to amplify a segment of the coat protein gene of the pmwav-2. the outer primers were designed to anneal at higher temperatures than the nested primers to prevent primer ...201222484612
optimization of pineapple pulp residue hydrolysis for lipid production by rhodotorula glutinis tistr5159 using as biodiesel feedstock.the higher lipid productivity of rhodotorula glutinis tistr5159 was achieved by optimizing the pineapple pulp hydrolysis for releasing the high sugars content. the sequential simplex method operated by varied; solid-to-liquid ratio, sulfuric acid concentration, temperature, and hydrolysis time were successfully applied and the highest sugar content (83.2 g/l) evaluated at a solid-to-liquid ratio of 1:10.8, 3.2% sulfuric acid, 105 °c for 13.9 min. moreover, the (nh4)2so4 supplement enhanced the l ...201627149319
table wine from tropical fruits utilizing natural yeast isolates.an attempt was made to utilize few widely available tropical fruits to develop wine with the objective of comparing the fermentation efficiency (along with progress in fermentation) of two efficient yeast isolates with commercially available strain. fruit wine from juices of fully ripe mango, jackfruit and pineapple alone and in blended combinations of all three fruit juice (2: 1: 2) was prepared using two different yeasts (y4 and y7) isolated from natural plain date palm juice and one standard ...201627570291
study of the antifungal potential of novel cellulose/copper composites as absorbent materials for fruit juices.cellulose/copper composites with antifungal properties have been synthesized by physical/chemical methods. physical treatments by heat or by a combination of heat and uv radiation provided composites with metallic copper and excellent interfacial adhesion; in contrast, chemical reduction with borohydride generated small although partially aggregated copper oxide nanoparticles. copper micro/nano-particles and copper ions (cu(2+)) were released from the cellulose matrix at an adequate rate to achi ...201222835229
alcohol production from pineapple waste.saccharomyces cerevisiae andzymomonas mobilis were grown on pineapple waste and their alcohol production characteristics compared. the pineapple waste consisted of 19% cellulose, 22% hemi-cellulose, 5% lignin and 53% cell soluble matters but concentration of soluble sugars, which included 5.2% sucrose, 3.1% glucose and 3.4% fructose, was relatively low and pretreatment of the substrate was needed. pretreatment of pineapple waste with cellulase and hemi-cellulase and then fermantation withs. cere ...199024430066
optimized butyl butyrate synthesis catalyzed by thermomyces lanuginosus lipase.butyl butyrate is an ester present in pineapple flavor, which is very important for the food and beverages industries. in this work, the optimization of the reaction of butyl butyrate synthesis catalyzed by the immobilized lipase lipozyme tl-im was performed. n-hexane was selected as the most appropriate solvent. other reaction parameters such as temperature, substrate molar ratio, biocatalyst content and added water, and their responses measured as yield, were evaluated using a fractional facto ...201323946156
inducible expression of bs2 r gene from capsicum chacoense in sweet orange (citrus sinensis l. osbeck) confers enhanced resistance to citrus canker disease.transgenic expression of the pepper bs2 gene confers resistance to xanthomonas campestris pv. vesicatoria (xcv) pathogenic strains which contain the avrbs2 avirulence gene in susceptible pepper and tomato varieties. the avrbs2 gene is highly conserved among members of the xanthomonas genus, and the avrbs2 of xcv shares 96% homology with the avrbs2 of xanthomonas citri subsp. citri (xcc), the causal agent of citrus canker disease. a previous study showed that the transient expression of pepper bs ...201728155188
transgenic sweet orange plants expressing a dermaseptin coding sequence show reduced symptoms of citrus canker disease.citrus canker provoked by xanthomonas axonopodis pv. citri is a bacterial disease causing severe losses in all citrus-producing areas around the world. xanthomonas infection is considered as an endemic disease in northeast and northwest argentina, affecting as much as 10% of commercial citrus plantations. there is not known natural resistance neither in orange varieties nor in rootstocks used for grafting of commercial cultivars. to introduce resistance to this disease, plants of pineapple sweet ...201323896218
novel approach of using nutraceutic-directed caloric antioxidant density and ion-ratio for evaluating fruit's health quality.seven kinds of indigenous fruits and five imported fruits were compared for their "health quality." methods including the calorific, antioxidant, and ion ratios were carried out. results indicated the order of content (in mg/100g) was: ca(2+) , murcott orange (218.2) > kiwifruit (200.0) > pineapple (138.5) > golden kiwi (117.6); mg(2+) , pitaya (192.2), banana (88.0), kiwifruit (63.4), and golden kiwi (58.4); zn(2+) , pitaya (19.53) > pear (10.8) > kiwifruit (6.09) > irwin mango (4.58). cu(2+) , ...201627467811
comparative analysis on the distribution of protease activities among fruits and vegetable resources.in this study, a comparative analysis on the distribution of protease activities among 90 plant resources, including fruits and vegetables, has been performed. protease activities of plant extracts were assayed at different ph values (ph 3.0, ph 7.5 and ph 10.5) using casein as a substrate. ten fruits and thirteen vegetables show protease activities above 10u/g. pineapple, fig and papaya, which are used for commercial protease production, exhibited high protease activities. additionally, high pr ...201627451238
application of membrane separation in fruit and vegetable juice processing: a review.fruit and vegetable juices are used due to convenience. the juices are rich in various minerals, vitamins, and other nutrients. to process the juices and their clarification and/or concentration is required. the membranes are being used for these purposes. these processes are preferred over others because of high efficiency and low temperature. membranes and their characteristics have been discussed in brief for knowing suitability of membranes for fruit and vegetable juices. membrane separation ...201524915352
survey of quality indicators in commercial dehydrated fruits.physical and chemical quality parameters (dry matter, aw, protein, carbohydrates, vitamin c, 2-furoylmethyl amino acids, rehydration ratio and leaching loss) have been determined in 30 commercial dehydrated fruits (strawberry, blueberry, raspberry, cranberry, cherry, apple, grapefruit, mango, kiwifruit, pineapple, melon, coconut, banana and papaya). for comparison purposes, strawberry samples processed in the laboratory by freeze-drying and by convective drying were used as control samples. over ...201424360417
the relative anthelmintic efficacy of plant-derived cysteine proteinases on intestinal nematodes.we examined the in vitro and in vivo efficacy of plant cysteine proteinases (cps) derived from pineapple (ananas comosus) and kiwi fruit (actinidia deliciosa), and compared their efficacy as anthelmintics to the known effects of cps from the latex of papaya (carica papaya) against the rodent intestinal nematode, heligmosomoides bakeri. both fruit bromelain and stem bromelain had significant in vitro detrimental effects on h. bakeri but in comparison, actinidain from kiwi fruit had very little ef ...201524176056
effect of acid fruit juices combined with electric or sonic toothbrushing on root dentin permeability--an in vitro study.the purpose of this in vitro study was to quantify the alterations on human root dentin permeability after exposure to different acid fruit juices and to evaluate the effect of toothbrushing with electric or sonic toothbrush after acid exposure. the root dentin of 50 extracted third molars was exposed with a high speed bur. crowns were sectioned above the cementoenamel junction and root fragments were used to prepare dentin specimens. specimens were randomly assigned to 5 groups according to the ...201223338258
carotenoids and color of fruit juice and milk beverage mixtures.seventeen commercially available, "ready to drink" fortified beverages consisting of mixtures of fruit juices and milk were analyzed to evaluate their carotenoid profile (including their z/e stereoisomers) and color during their commercial shelf life. lightness (l*) was found to be correlated with the content of milk in the mixtures (r= 0.649) whereas red-yellow colors were correlated with the contents of alpha-carotene, beta-cryptoxanthin, and beta-carotene supplied by the fruit. the beverages ...200718034704
quality changes and nutrient retention in fresh-cut versus whole fruits during storage.the influences of processing and storage on the quality indices and nutritional content of fresh-cut fruits were evaluated in comparison to whole fruits stored for the same duration but prepared on the day of sampling. fresh-cut pineapples, mangoes, cantaloupes, watermelons, strawberries, and kiwifruits and whole fruits were stored for up to 9 days in air at 5 degrees c. the postcutting life based on visual appearance was shorter than 6 days for fresh-cut kiwifruit and shorter than 9 days for fr ...200616756358
dengue viruses in aedes albopictus skuse from a pineapple plantation in costa rica. 201526047200
fluctuation of diptera larvae in phytotelmata and relation with climate variation in west sumatra indonesia.research of fluctuations in diptera's larvae in phytotelmata had been conducted at three locations in west sumatra, indonesia; padang, bukittinggi and payakumbuh; which aimed to determine the number and fluctuations diptera larvae in phytotelmata. the results obtained; the highest number of individual larvae diptera in phytotelmata was 7109 aedes albopictus larvae (49.56%), followed by larvae of culex tritaeniorhynchus with 2409 individuals (16.80%). larvae fluctuated every month and tent to inc ...201426035947
ethnopharmacological survey of medicinal plants used by indigenous and tribal people in rangamati, bangladesh.there is very limited information regarding plants used by traditional healers in rangamati, bangladesh, for treating general ailments. current study provides significant ethnopharmacological information, both qualitative and quantitative on medical plants in rangamati.201223064284
agave as a model cam crop system for a warming and drying world.as climate change leads to drier and warmer conditions in semi-arid regions, growing resource-intensive c3 and c4 crops will become more challenging. such crops will be subjected to increased frequency and intensity of drought and heat stress. however, agaves, even more than pineapple (ananas comosus) and prickly pear (opuntia ficus-indica and related species), typify highly productive plants that will respond favorably to global warming, both in natural and cultivated settings. with nearly 200 ...201526442005
in vivo effect of selected medicinal plants against gastrointestinal nematodes of sheep.nematode resistance to anthelmintic drugs affects small ruminant production globally. this study evaluated in vivo effects of five plant extracts as alternative nematode treatments. animals were divided into six groups. each group was randomly assigned a treatment: abamectin and praziquantel (cap) (positive control), ethanol extracts of ananas comosus (ac), aloe ferox (af), allium sativum (as), lespedeza cuneata (lc) and warburgia salutaris (ws). these were applied as an oral dose (100 mg kg(-1) ...201424293151
[vitamin c in fruits and vegetables].strong opinion about reducing vitamin c content in traditional cultivars of fruits and vegetables as a result of intensive farming practices, on the one hand, and depletion of soil, waste of fertilizers, on the other hand, takes place. the aim of the study was to assess changes in vitamin c content in fresh vegetables, fruits and berries from the 40s of last century to the present. available national and foreign data from official tables of the chemical composition tables published in different ...201324006751
factors significantly increasing or inhibiting early stages of malignant melanoma (m.m.) and non-invasive evaluation of new treatment by ingestion and external application of optimal doses of the most effective anti-m.m. substances: haritaki, cilantro, vitamin d3, nori, epa with dha, & application of special (+) solar energy stored paper, which reduced the m.m. active area & asbestos rapidly.sterilizing the pre-cancer skin of malignant melanoma (m.m.) with 70% isopropyl alcohol intensified malignancy & the malignant response extended to surrounding normal looking skin, while sterilizing with 80% (vodka) or 12% (plum wine) ethyl alcohol completely inhibited m.m. in the area (both effects lasted for about 90 minutes initially). burnt food (bread, vegetables, meat, and fish), a variety of smoked & non-smoked fish-skin, many animal's skin, pepper, vitamin c over 75 mg, mango, pineapple, ...201323724697
mycotoxins biosynthesized by plant-derived fusarium isolates.there is little information on secondary metabolites produced by fusaria infecting crop plants other than cereals. many members of fusarium genus have the ability to colonise perennial crops with only scarce infection or disease symptoms or with no symptoms at all while still being detectable. even in case of such asymptomatic infection, significant mycotoxin contamination of the plant tissues is possible. the aim of this study was to characterise the spectrum of fusarium species isolates obtain ...201223334038
diet intake patterns of non-bengali muslim mothers during pregnancy and lactation.an explorative study was carried out to ascertain the knowledge, attitudes, and practices regarding diet patterns during pregnancy and lactation among non-bengali muslim mothers. 100 consecutive mothers admitted for delivery in the obstetric ward of islamia hospital, calcutta, were selected as index cases. retrospective data from these mothers were collected about their diet patterns during pregnancy. at least 3 home visits were made for each mother after her discharge from the hospital: the 1st ...20162641755
juice blends--a way of utilization of under-utilized fruits, vegetables, and spices: a review.the post-harvest shelf life of maximum of fruits and vegetables is very limited due to their perishable nature. in india more then 20-25 percent of fruits and vegetables are spoiled before utilization. despite being the world's second largest producer of fruits and vegetables, in india only 1.5 percent of the total fruits and vegetables produced are processed. maximum amounts of fruit and vegetable juices turn bitter after extraction due to conversion of chemical compounds. in spite of being und ...201121929332
relationships between stomatal behavior and internal carbon dioxide concentration in crassulacean acid metabolism plants.measurements of internal gas phase co(2) concentration, stomatal resistance, and acid content were made in crassulacean acid metabolism plants growing under natural conditions. high co(2) concentrations, sometimes in excess of 2%, were observed during the day in a range of taxonomically widely separated plants (opuntia ficus-indica l., opuntia basilaris engelm. and bigel., agave desertii engelm., yucca schidigera roezl. ex ortiges, ananas comosus [l.] merr., aloe vera l., cattleya sp. and phalan ...197916660851
the association between chromaticity, phenolics, carotenoids, and in vitro antioxidant activity of frozen fruit pulp in brazil: an application of chemometrics.a total of 19 brazilian frozen pulps from the following fruits: açai (euterpe oleracea), blackberry (rubus sp.), cajá (spondias mombin), cashew (anacardium occidentale), cocoa (theobroma cacao), coconut (cocos nucifera), grape (vitis sp.), graviola (annona muricata), guava (psidium guajava), papaya (carica papaya), peach (prunus persica), pineapple (ananas comosus), pineapple and mint (a. comosus and mentha spicata), red fruits (rubus sp. and fragaria sp.), seriguela (spondias purpurea), strawbe ...201424547813
plant proteases for bioactive peptides release: a review.proteins are a potential source of health promoting biomolecules with medical, nutraceutical and food applications. nowadays, bioactive peptides production, its isolation, characterization and strategies for its delivery to target sites are a matter of intensive research. in vitro and in vivo studies regarding the bioactivity of peptides has generated strong evidence of their health benefits. dairy proteins are considered the richest source of bioactive peptides, however proteins from animal and ...201728394630
monodispersed gold nanoparticles as a probe for the detection of hg2+ ions in water.gold nanoparticles were synthesized using ananas comosus as reducing agent. uv-visible spectra show the surface plasmon resonance peak at 544 nm. tem measurement shows that the formation of monodispersed spherical nanoparticles with average size of 7 nm. crystalline nature of the nanoparticles was evident from tem images and peaks in the xrd pattern. ftir analysis provides the presence of biomolecules responsible for the reduction and capping of the prepared gold nanoparticles. a selective and s ...201728380237
simple fabrication of pineapple root-like palladium-gold catalysts as the high-efficiency cathode in direct peroxide-peroxide fuel cells.pd-au/tic electrodes with various three-dimensional structures are obtained by the pulsed potential electro-deposition in pdcl2/haucl4 electrolytes. the morphologies of pd-au/tic composite catalysts are significantly dependent on the component of deposited solutions. the surface appearance of pd-au catalysts changes from rime-shaped structure, to feather-like construction, then to pineapple root-like structure and finally to flower-like configuration with the increase of pdcl2 content in electro ...201728342307
physicochemical changes of 'phulae' pineapple fruit treated with short-term anoxia during ambient storage.the effects of short-term anoxia exposure for 16h on physicochemical changes of 'phulae' pineapple fruit stored at ambient temperature (25±2°c) were investigated. the respiratory rate of the fruit was induced by the anoxia treatment. however, it retarded the increase in moisture loss and maintained both flesh and pulp colour by inhibiting polyphenol oxidase (ppo) activity of the both tissues. the anoxia exposure delayed the increase in total sugar content and enhanced total ascorbic acid content ...201728317739
using high hydrostatic pressures to retain the antioxidant compounds and to reduce the enzymatic activity of a pitaya-pineapple (stenocereus sp.-fragaria ananassa) beverage.pitaya (stenocereus sp.) is a fruit native to arid and semiarid areas of mexico. it has high antioxidant activity mainly due to its contents of betalains and phenolics, but its consumption is limited due to very short shelf-life and not very recognized flavor. a beverage of pitaya and pineapple was formulated to improve sensory properties. a high hydrostatic pressure (hhp) study at 400-600 mpa and 25 °c for 2-10 min was applied in the beverage and the effect on the contents of vitamin c, total p ...201728298674
sex pheromone of a coccoid insect with sexual and asexual lineages: fate of an ancestrally essential sexual signal in parthenogenetic females.sex pheromones play a central role in intersexual communication for reproduction in many organisms. particularly in insects, reproductive isolation that leads to speciation is often achieved by shifts of pheromone chemistries. however, the divergence and evolution of pheromones remain largely unknown. this study reveals a unique evolutionary consequence for terpenoid pheromones in coccoid insects. coccoids, such as mealybugs, show clear sexual dimorphism: males are dwarf and short-lived, whereas ...201728250102
influence of the fiber from agro-industrial co-products as functional food ingredient on the acceptance, neophobia and sensory characteristics of cooked sausages.the sensory analysis of new products is essential for subsequent acceptance by consumers, moreover in the functional food market. the acceptance and food neophobia of cooked sausages formulated with cactus pear fiber or pineapple pear fiber, as functional ingredient, was complemented with a sensory characterization by r-index and qualitative descriptive analysis (qda). female consumers aged between 40 and 50 years showed greater interest in the consumption of healthy foods, with a higher level o ...201728242937
the proteolytic system of pineapple stems revisited: purification and characterization of multiple catalytically active forms.crude pineapple proteases extract (aka stem bromelain; ec 3.4.22.4) is an important proteolytic mixture that contains enzymes belonging to the cysteine proteases of the papain family. numerous studies have been reported aiming at the fractionation and characterization of the many molecular species present in the extract, but more efforts are still required to obtain sufficient quantities of the various purified protease forms for detailed physicochemical, enzymatic and structural characterizatio ...201728238440
a comparative analysis of the chemical composition, anti-inflammatory, and antinociceptive effects of the essential oils from three species of mentha cultivated in romania.this work was aimed at correlating the chemotype of three mentha species cultivated in romania with an in vivo study of the anti-inflammatory and antinociceptive effects of essential oils. the selected species were mentha piperita l. var. pallescens (white peppermint), mentha spicata l. subsp. crispata (spearmint), and mentha suaveolens ehrh. (pineapple mint). qualitative and quantitative analysis of the essential oils isolated from the selected mentha species was performed by gas chromatography ...201728208614
management of oral secretions in neurological disease.sialorrhoea is a common and problematic symptom that arises from a range of neurological conditions associated with bulbar or facial muscle dysfunction. drooling can significantly affect quality of life due to both physical complications such as oral chapping, and psychological complications such as embarrassment and social isolation. thicker, tenacious oral and pharyngeal secretions may result from the drying management approach to sialorrhoea. the management of sialorrhoea in neurological dise ...201728188210
measuring population fluctuation of jatropha stem-borer [cophes notaticeps (marshall)] in the brazilian cerrado using a new trap.this study aimed to monitor the population fluctuation of cophes notaticeps (marshall) (coleoptera: curculionidae) in a jatropha (jatropha curcas l.) plantation in the federal district, brazil, through the use of a new trap, combined with different attractive lures and trap colors. the study was conducted at embrapa cerrados (planaltina/df) in a field trial with 720 plants. the new trap, named cpac16, was made with pvc pipes (100 mm in diameter) of about 40 cm in length, with a window (8 × 10 cm ...201728185606
phylogenomic relationship of feijoa (acca sellowiana (o.berg) burret) with other myrtaceae based on complete chloroplast genome sequences.given their distribution, importance, and richness, myrtaceae species comprise a model system for studying the evolution of tropical plant diversity. in addition, chloroplast (cp) genome sequencing is an efficient tool for phylogenetic relationship studies. feijoa [acca sellowiana (o. berg) burret; cn: pineapple-guava] is a myrtaceae species that occurs naturally in southern brazil and northern uruguay. feijoa is known for its exquisite perfume and flavorful fruits, pharmacological properties, o ...201728185042
comparison between titrimetric and spectrophotometric methods for quantification of vitamin c.vitamin c is usually quantified by titrimetric or chromatographic methods. however, these methods have limitations: food color interferes with the titrimetric method and the chromatographic method is costly. the aim of this study was to compare a spectrophotometric method, based on reduction of cupric ions in the presence of cuproine complex, with a titrimetric method, based on reduction of 2,6-dicholorophenolindophenol. linearity, precision, accuracy, and limits of detection (lod) and quantific ...201728159298
evidence of some natural products with  antigenotoxic effects. part 1: fruits and  polysaccharides.cancer is one of the leading causes of deaths worldwide. the agents capable of causing damage to genetic material are known  as genotoxins and, according to their mode of action, are classified into mutagens, carcinogens or teratogens. genotoxins are  involved in the pathogenesis of several chronic degenerative diseases including hepatic, neurodegenerative and cardiovascular  disorders, diabetes, arthritis, cancer, chronic inflammation and ageing. in recent decades, researchers have found novel ...201728157162
pesticide exposure and cholinesterase levels in migrant farm workers in thailand.in this study, we examined the effects of pesticides in migrant farm workers from cambodia after workplace exposure on fruit plantations in eastern thailand.201728139185
using a radial diffusion method to investigate the role of plasmin degradation of fibrin in a physical model of an early-phase wound.fibrin clot formation, which acts to stabilize a wound following injury, is among the key early aspects of dermal wound healing. this preliminary matrix is eventually degraded via a process known as fibrinolysis and replaced with a collagen-rich matrix that continues to be remodeled to minimize scarring. disruptions in these carefully coordinated events lead to certain undesirable conditions such as fibrosis and the formation of abnormal scars that are associated with excess amounts of collagen. ...201728135202
a method for measuring orientation within a magnetic resonance imaging scanner using gravity and the static magnetic field (vectorient).in mri brain imaging, subject motion limits obtainable image clarity. due to the hardware layout of an mri scanner, gradient excitations can be used to rapidly detect position. orientation, however, is more difficult to detect and is commonly calculated by comparing the position measurements of multiple spatially constrained points to a reference dataset. the result is increased size of the apparatus the subject must wear, which can influence the imaging workflow. in optical based methods marker ...201728129151
pineelm_ssrdb: a microsatellite marker database identified from genomic, chloroplast, mitochondrial and est sequences of pineapple (ananas comosus (l.) merrill).simple sequence repeats or microsatellites are resourceful molecular genetic markers. there are only few reports of ssr identification and development in pineapple. complete genome sequence of pineapple available in the public domain can be used to develop numerous novel ssrs. therefore, an attempt was made to identify ssrs from genomic, chloroplast, mitochondrial and est sequences of pineapple which will help in deciphering genetic makeup of its germplasm resources.201628096778
physiological characterization and comparative transcriptome analysis of white and green leaves of ananas comosus var. bracteatus.leaf coloration is one of the most important and attractive characteristics of ananas comosus var. bracteatus. the chimeric character is not stable during the in vitro tissue culturing. many regenerated plants lost economic values for the loss of the chimeric character of leaves. in order to reveal the molecular mechanisms involved in the albino phenotype of the leaf cells, the physiological and transcriptional differences between complete white (cwh) and green (cgr) leaf cells of a. comosus var ...201728095462
side streams of plant food processing as a source of valuable compounds: selected examples.industrial processing of plant-derived raw materials generates enormous amounts of by-products. on one hand, these by-products constitute a serious disposal issue because they often emerge seasonally and are prone to microbial decay. on the other hand, they are an abundant source of valuable compounds, in particular secondary plant metabolites and cell wall materials, which may be recovered and used to functionalize foods and replace synthetic additives with ingredients of natural origin. this r ...201728068488
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