| experimental study of the hypoglycemic effect of some antidiabetic plants. | the purpose of this work is to look for the hypoglycemic effect of 12 plants most used in mexico for controlling diabetes mellitus. the studies were realized in 27 rabbits submitted weekly to glucose tolerance tests after gastric administration of water, tolbutamide or a preparation of the plant. the results showed that tolbutamide and studied plants (except aloe barbadensis) decreased significantly (p less than 0.05) the area under glucose tolerance curve, in relation with the water control. th ... | 1991 | 1819981 |
| vascular-specific expression of the bean grp 1.8 gene is negatively regulated. | in french bean, the glycine-rich cell wall protein grp 1.8 is specifically synthesized in the vascular tissue. to identify cis-acting sequences required for cell type-specific synthesis of grp 1.8, expression patterns of fusion gene constructs were analyzed in transgenic tobacco. in these constructs, the uida (beta-glucuronidase) gene was placed under control of 5' upstream deletions as well as internal deletions of the grp 1.8 promoter. four different cis-acting regulatory regions, se1 and se2 ... | 1991 | 1821759 |
| regulation of glycan processing by golgi enzymes from red kidney bean (phaseolus vulgaris) seedlings. | the effect of a protein matrix on the processing of glycoprotein glycans by golgi enzymes from plant seedlings has been determined with an artificial glycoprotein system, comparing the processing rates of glycan-(biotinyl)asn (or glycan-(biotinamidohexanoyl)asn) substrates either free or bound to avidin. an analysis of the pooled glycoproteins from the seedlings suggested that the most common glycan structure is a complex one (glcnac-man3glycnac2-protein), and consistent with this processing end ... | 1991 | 1832830 |
| induced plant responses to pathogen attack. analysis and heterologous expression of the key enzyme in the biosynthesis of phytoalexins in soybean (glycine max l. merr. cv. harosoy 63). | in soybean (glycine max l.), pathogen attack induces the formation of glyceollin-type phytoalexins. the biosynthetic key enzyme is a reductase which synthesizes 4,2', 4'-trihydroxychalcone in co-action with chalcone synthase. screening of a soybean cdna library from elicitor-induced rna in lambda gt11 yielded two classes of reductase-specific clones. the deduced proteins match to 100% and 95%, respectively, with 229 amino acids sequenced in the purified plant protein. four clones of class a were ... | 1991 | 1840523 |
| [evaluation of two methods to determine the polyphenol content in raw and cooked beans and its effect on protein digestibility]. | the polyphenolic compounds present in raw and cooked, and dried, with and without the cooking broth of common white, black and red beans (phaseolus vulgaris) were measured by the folin-denis method for total polyphenols, and by the protein precipitation method of hagerman-butler, which measures their biological activity. the polyphenol content was measured during 20 consecutive days on the same sample, using three different extracts of volume from each sample. statistical analysis of the results ... | 1991 | 1841538 |
| different legumin protein domains act as vacuolar targeting signals. | legumin subunits are synthesized as precursor polypeptides and are transported into protein storage vacuoles in field bean cotyledons. we expressed a legumin subunit in yeast and found that in these cells it is also transported into the vacuoles. to elucidate vacuolar targeting information, we constructed gene fusions of different legumin propolypeptide segments with either yeast invertase or chloramphenicol acetyltransferase as reporters for analysis in yeast or plant cells, respectively. in ye ... | 1991 | 1841724 |
| genetic and transcriptional organization of the hrp cluster of pseudomonas syringae pv. phaseolicola. | the hrp cluster of pseudomonas syringae pv. phaseolicola encodes functions that are essential for pathogenicity on bean plants and for the elicitation of the hypersensitive response on resistant plants. the cluster was saturated with insertions of transposon tn3-spice that served both as a mutagen and as a sensitive reporter of the expression of the target regions. the mutations covered a 17.5-kb segment in strain nps3121, in which seven hrp::tn5 insertions had been previously mapped, and region ... | 1991 | 1846144 |
| stress responses in alfalfa (medicago sativa l.). 8. cis-elements and trans-acting factors for the quantitative expression of a bean chalcone synthase gene promoter in electroporated alfalfa protoplasts. | a chimeric gene consisting of a bean (phaseolus vulgaris l.) chalcone synthase (chs) promoter fused to a bacterial chloramphenicol acetyltransferase (cat) reporter gene was strongly expressed, and further induced by fungal elicitor, when electroporated into alfalfa (medicago sativa l.) suspension cell protoplasts. functional analysis of 5' deletions of the chs promoter-cat construct in these protoplasts indicated that the region between -326 and -130 contained both activator and silencer element ... | 1991 | 1859870 |
| projections of the parvocellular reticular formation to the contralateral mesencephalic trigeminal nucleus in the rat. | projections of the parvocellular reticular nucleus (pcrt) to the contralateral mesencephalic trigeminal nucleus (me5) were studied in the rat with neurophysiological and neuroanatomical techniques. three types of responses (classified by latencies) were recorded extracellularly in the me5 area after electrical stimulation of the pcrt: (1) r1 fast unitary reactions (latency 0.2-0.5 ms) found throughout the entire caudal me5 area; (2) r2 slower unitary reactions (latency 0.7-1.2 ms) recorded ventr ... | 1991 | 1860066 |
| the effect of the consumption of red kidney beans on the growth rate of rats and the implications for human populations. | | 1991 | 1861267 |
| binding of kidney bean (phaseolus vulgaris) isolectins to differentiated human colon carcinoma caco-2 cells and their effect on cellular metabolism. | the binding of phaseolus vulgaris (pha) isolectins l4 and e4 to the brush border membrane of differentiated caco-2 cells was studied and the impact on cellular metabolism and microvilli was assessed. computer analysis of the data based on binding experiments with peroxidase conjugated isolectins gave mean (sd) values for maximal binding of 2540 (151).10(-9) m for pha-l4 and 2104 (140).10(-9) m for pha-e4 per mg of brush border membrane protein. the dissociation constants for l4 and e4 binding we ... | 1991 | 1864541 |
| reinnervation of cerebellar purkinje cells by climbing fibres surviving a subtotal lesion of the inferior olive in the adult rat. i. development of new collateral branches and terminal plexuses. | cerebellar climbing fibres react by collateral sprouting after subtotal lesions of the inferior olive, and the newly formed branches are able to reinnervate neighbouring denervated purkinje cells. in the present paper, we used the phaseolus vulgaris leucoagglutinin (pha-l) tracing technique to label the climbing fibres and study their plasticity in detail at the light microscopical level. the specific objectives were to study the time course and morphological aspects of their sprouting, to estim ... | 1991 | 1865015 |
| reinnervation of cerebellar purkinje cells by climbing fibres surviving a subtotal lesion of the inferior olive in the adult rat. ii. synaptic organization on reinnervated purkinje cells. | a salient feature of the cerebellar purkinje cells is the highly ordered distribution of their excitatory afferents on the dendritic tree. climbing fibres synapse exclusively on the proximal dendrites, whereas parallel fibres articulate with the distal branches, the so-called spiny branchlets. this input organization is lost following the removal of climbing fibres. such denervation results in the formation of a large number of new spines on the proximal dendrites, and these become contacted by ... | 1991 | 1865016 |
| the efferent connections of the lateral septal nucleus in the guinea pig: projections to the diencephalon and brainstem. | the anterograde phaseolus vulgaris-leucoagglutinin (pha-l) tracing technique was used to determine the distribution of efferent fibers originating in the lateral septal nucleus of the guinea pig. for complementary detection of the chemical identity of the target neurons, double-labeling immunocytochemistry was performed with antibodies to pha-l and to vasopressin, oxytocin, vasoactive intestinal polypeptide, serotonin or dopamine beta-hydroxylase, respectively. the hypothalamus received the majo ... | 1991 | 1868517 |
| the efferent connections of the lateral septal nucleus in the guinea pig: intrinsic connectivity of the septum and projections to other telencephalic areas. | the distribution of efferent fibers originating in the lateral septal nucleus was investigated in guinea pigs by means of anterograde tracing with phaseolus vulgaris-leucoagglutinin (pha-l). special emphasis was placed on the intraseptal fiber systems. the fibers originating from the different subnuclei of the lateral septal nucleus formed massive horizontal connections in the rostrocaudal axis. projections to the contralateral, congruent subnuclei were also detected. in the medial septum/diagon ... | 1991 | 1868518 |
| parabrachial nucleus projection towards the hypothalamic supraoptic nucleus: electrophysiological and anatomical observations in the rat. | it has been proposed that the pontine parabrachial nucleus (pbn) participates in the regulation of body fluid balance and the release of vasopressin from the neurohypophysis, although the pathways mediating the latter response are uncertain. this study in the rat, utilizing anatomical and electrophysiological methods, describes a projection from the lateral pbn towards the hypothalamic supraoptic nucleus (son). rats received iontophoretic injections of the anterograde tracer phaseolus vulgaris l ... | 1991 | 1874981 |
| morphology and axonal projection patterns of individual neurons in the cat perigeniculate nucleus. | 1. the lateral geniculate nucleus is the primary thalamic relay through which retinal signals pass en route to cortex. this relay is gated and can be suppressed by activity among local inhibitory neurons that use gamma-aminobutyric acid (gaba) as a neurotransmitter. in the cat, a major source of this gabaergic inhibition seems to arise from cells of the perigeniculate nucleus, which lies just dorsal to the a-laminae of the lateral geniculate nucleus. however, the morphological characteristics of ... | 1991 | 1875260 |
| [negative effects of anti-nutritional factors in animal feed on the nitrogen burden of the environment and possibilities of its reduction. pathological effects]. | some pathological effects of lectins in legume seeds, particular by phasoleus vulgaris (red kidney bean) agglutinins, on intestinal epithelial cell structure and function in a bio-assay and in various in vivo ('self-emptying blind pouch-model') and in vitro (cell- and organ culture) models are reported. | 1991 | 1882368 |
| the structure of a phaseolus vulgaris cdna encoding the iron storage protein ferritin. | a cdna containing the entire coding region for the iron storage protein ferritin has been isolated from the french bean plant, phaseolus vulgaris l. cv. tendergreen. ferritin protein was purified from young leaves and shoot meristem tissue and used to raise antisera in mice. a lambda gt11 cdna library was constructed from seed-derived poly(a)+ rna, and screened with the mouse anti-ferritin serum. a 1.2 kb immunopositive phage dna insert was isolated and sequenced. the derived amino acid sequence ... | 1991 | 1884000 |
| the projection of noradrenergic neurons in the a7 catecholamine cell group to the spinal cord in the rat demonstrated by anterograde tracing combined with immunocytochemistry. | noradrenergic neurons located in the a5, a7 and locus coeruleus/subcoeruleus (lc/sc) catecholamine cell groups innervate all levels of the spinal cord. however, the specific spinal cord terminations of these neurons have not been clearly delineated. this study determined the spinal cord terminations of the a7 catecholamine cell group using the anterograde tracer phaseolus vulgaris leucoagglutinin (pha-l) in combination with dopamine-beta-hydroxylase (dbh) immunocytochemistry. in addition, the sp ... | 1991 | 1884202 |
| descending input from the hypothalamic paraventricular nucleus to sympathetic preganglionic neurons in the rat. | the descending projection of the hypothalamic paraventricular nucleus (pvn) to the sympathetic preganglionic neurons (spns) in the upper thoracic cord of the rat was studied. pvn-fibers were labeled by anterograde transport of phaseolus vulgaris leucoagglutinin (pha-l), while spns were retrogradely labeled with cholera toxin subunit b (ctb) which was injected into the superior cervical ganglion. spns labeled with ctb were mainly observed in the nucleus intermediolateralis (iml) pars principalis ... | 1991 | 1884750 |
| pathological effects of phaseolus vulgaris isolectins on pig jejunal mucosa in organ culture. | the interaction of plant lectins with pig small intestinal epithelium in organ culture was studied. the binding of phaseolus vulgaris (pha) isolectins e4 and l4 to the microvilli and microvillus vesicles in the top area of the villi was shown by immunoelectron microscopy. differences were observed in the distribution of the isolectins. in the explants cultured for five hours with the pha isolectins, the enterocyte height and the villus length were decreased, and a lower villus: crypt ratio was c ... | 1991 | 1885071 |
| a projection of the lens accommodation-related area to the pupillo-constrictor area in the posteromedial lateral suprasylvian area in cats. | the fiber connection between areas related to lens accommodation and to pupillary constriction was studied in the posteromedial lateral suprasylvian area (pmls) in the extrastriate cortex of the cat. the accommodation-related area was located in the pmls by microstimulation, and the anterograde tracer phaseolus vulgaris leucoagglutinin was injected into this area. anterogradely-labeled fibers and terminals were found in the upper part of the medial bank of the middle suprasylvian sulcus, the are ... | 1991 | 1895565 |
| cdna cloning and characterization of a putative 1,3-beta-d-glucanase transcript induced by fungal elicitor in bean cell suspension cultures. | synthetic oligonucleotides based on similarity between tobacco 1,3-beta-d-glucanase and barley 1,3-1,4-beta-d-glucanase were used to prime the synthesis and amplification of a 162 bp bean (phaseolus vulgaris l.) beta-glucanase cdna by the polymerase chain reaction (pcr). the pcr product was used to isolate a near full-length beta-glucanase cdna corresponding to an approximately 1400 bp full-length transcript, from a library containing cdna sequences complementary to mrna from fungal elicitor-tre ... | 1991 | 1909591 |
| trajectory of redirected corticospinal axons after unilateral lesion of the sensorimotor cortex in neonatal rat; a phaseolus vulgaris-leucoagglutinin (pha-l) tracing study. | the corticospinal neurons of the rat project almost exclusively to the contralateral spinal cord. retrograde and anterograde tracing experiments showed that only about 2-4% of the corticospinal neurons of the sensorimotor cortex project to the ipsilateral spinal cord in the normal rat. the large majority of corticospinal axons (more than 90%) travel at spinal level at the base of the contralateral dorsal funiculus; in addition a few axons run in the contralateral lateral funiculus and at the bas ... | 1991 | 1915735 |
| further studies of lectin binding by villous and extravillous trophoblast in normal and pathological pregnancy. | examination of human pregnancy tissues with a panel of lectins provides the opportunity to probe different aspects of carbohydrate structure. nine biotinylated lectins [concanavalin a (con a), wheat germ agglutin (wga), lens culinaris a (lch-a), pisum sativum (psa), phaseolus vulgaris (pha-e and pha-l), ulex europaeus 1 (uea1), griffonia simplicifolia (gsi and gsii)] were used to investigate the lectin binding of human trophoblast in normal, tubal, and molar pregnancy. all lectins except uea1 bo ... | 1991 | 1917274 |
| [the degradation of vicine and convicine in field bean meal by selected bacterial strains]. | vicine and convicine as natural occurring toxic compounds in vicia faba are involved in the formation of hemolytic anemia (favism). removal of these compounds needs high technological expense. microbiological degradation with different strains of bacteria was tried. after 3 h incubation with streptococcus faeciens convicine was converted completely and vicine to about 95%. | 1991 | 1922274 |
| glycoconjugates of the lateral prostate of the guinea pig: a lectin histochemical study. | the present study examined the glycoconjugates of the lateral prostate using a battery of lectins. the results indicated that the secretory epithelium was rich in mannose (man), n-acetylglucosamine (glcnac), galactose (gal), n-acetylgalactosamine (galnac), and complex oligosaccharides. con a (concanavalin a), lca (lens culinaris agglutinin), psa (pisum sativum agglutinin), wga (wheat germ agglutinin), pwm (pokeweed mitogen), rca-i (racinus communis isolectin i), and pha-p (phaseolus vulgaris agg ... | 1991 | 1923963 |
| the effect of concentration of tannin-rich bean hulls (vicia faba l.) on activities of lipase (ec 3.1.1.3) and alpha-amylase (ec 3.2.1.1) in digesta and pancreas and on the digestion of lipid and starch by young chicks. | the effect of different concentrations of tannin-rich field-bean (vicia faba l.) hulls at 0, 20, 50, 150 and 300 g/kg dietary inclusion on the activities of lipase (ec 3.1.1.3) and alpha-amylase (ec 3.2.1.1) in digesta and pancreas and on the digestion of lipid and starch was studied in 2-3-week-old male broiler chicks. low dietary concentrations of tannins (20 and 50 g hulls/kg) enhanced the activity of lipase in digesta from both the jejunum and ileum, the 20 g hulls/kg effecting the greatest ... | 1991 | 1931901 |
| resonance raman spectra of plastocyanin and pseudoazurin: evidence for conserved cysteine ligand conformations in cupredoxins (blue copper proteins). | new resonance raman (rr) spectra at 15 k are reported for poplar (populus nigra) and oleander (oleander nerium) plastocyanins and for alcaligenes faecalis pseudoazurin. the spectra are compared with those of other blue copper proteins (cupredoxins). in all cases, nine or more vibrational modes between 330 and 460 cm-1 can be assigned to a coupling of the cu-s(cys) stretch with cys ligand deformations. the fact that these vibrations occur at a relatively constant set of frequencies is testimony t ... | 1991 | 1932014 |
| the globus pallidus receives a projection from the parafascicular nucleus in the rat. | study of the afferents of the rat globus pallidus (gp) with fluoro-gold, a retrograde tracer, revealed retrogradely labeled neurons in the ipsilateral parafascicular nucleus of the thalamus (pf), a previously undescribed afferent of the rat gp. we used the anterograde tracer, phaseolus vulgaris-leucoagglutinin (pha-l), to confirm and extend our findings. after injections of pha-l in the pf, labeled fibers with varicosities and terminal specializations were observed in the ipsilateral gp. the top ... | 1991 | 1933274 |
| changes in the expression of lectins in human t lymphocyte membrane upon mitogenic stimulation. | surface lectins, specific for given sugar structures, are expressed on human t cells, as shown by flow cytofluorometry using f-neoglycoproteins bearing either beta- and alpha-d-galactosyl, beta-d-galactosyl 6-phosphate, or alpha-l-rhamnosyl groups, but not by f-neoglycoproteins bearing other sugar groups (such as alpha-d-mannosyl groups). after stimulation with phaseolus vulgaris mitogen, the number of cells that bind beta-d-galactosyl 6-phosphate groups (6-p-beta-d-galp lec+ cells) increased fo ... | 1991 | 1933943 |
| complete amino acid sequence of endo-beta-n-acetylglucosaminidase from flavobacterium sp. | the complete amino acid sequence of endo-beta-n-acetylglucosaminidase from flavobacterium sp. has been determined by analysis of peptides after cleavage with lysyl endopeptidase, pepsin and chymotrypsin. the protein consists of a single polypeptide chain consisting of 267 amino acid residues and a molecular mass of 27972 da. the sequence of flavobacterium endo-beta-n-acetylglucosaminidase is very close to that of the streptomyces enzyme (endo-h), having 60% similarity and very similar hydropathy ... | 1991 | 1935974 |
| efferent connections of the striatopallidal and amygdaloid components of the substantia innominata in the cat: projections to the nucleus accumbens and caudate nucleus. | enkephalin immunoreactivity is used to divide the feline substantia innominata into circumscript subregions, i.e. the "striatopallidal system" and the "extended amygdala". in addition, enkephalin immunoreactivity is used to subdivide the striatopallidal system into two distinct areas, i.e. the subcommissural part of the globus pallidus displaying high enkephalin immunoreactivity and the ventral pallidum displaying moderate enkephalin immunoreactivity. the anterograde axonal transport of phaseolu ... | 1991 | 1944894 |
| individual c6 glioma cells migrate in adult rat brain after neural homografting. | cultured c6 glioma cells were prelabeled with the plant lectin phaseolus vulgaris leuco-agglutinin (phal) and grafted as a cell suspension (10(6) cells in 5.0 microliters) into freshly made cortical implantation pockets in adult host rats. animals were killed 1-21 days post-implantation (dpi). the brains were removed, dehydrated, embedded in paraffin and sectioned at 8 microns. paraffin sections were processed for light level immunofluorescent double labeling for phal, a marker for graft derived ... | 1991 | 1950656 |
| physical and chemical control of released microorganisms at field sites. | an important consideration in the environmental release of a genetically engineered microorganism is the capability for reduction or elimination of microorganism populations once their function is completed or if adverse environmental effects are observed. in this study the decontamination treatments of burning and biocide application, alone and in combination with tilling, were evaluated for their ability to reduce populations of bacteria released on the phylloplane. field plots of bush beans ( ... | 1991 | 1954582 |
| synaptic organization of cortico-cortical connections from the primary visual cortex to the posteromedial lateral suprasylvian visual area in the cat. | the synaptic organization of the projection from the cat striate visual cortex to the posteromedial lateral suprasylvian cortical area (pmls) was examined. the anterograde tracer phaseolus vulgaris leucoagglutinin (pha-l) was iontophorectically delivered into area 17, and anterogradely labeled fibers were revealed in pmls by means of an immunocytochemical detection method. most axons and presumptive terminal swellings were found in layers iii and iv. the neuronal elements (n = 190) that were pos ... | 1991 | 1955584 |
| characterization of gamma-glutamyltranspeptidase from human pancreatic cancer. | to elucidate the specific changes of pancreatic gamma-glutamyl-transpeptidase (gamma-gtp) associated with malignant transformation, some properties of gamma-gtp purified from pancreatic cancer were compared with those of gamma-gtps from normal pancreas and other tissues. four of five pancreatic cancer gamma-gtps showed distinctly slower electrophoretic mobility than normal pancreatic enzymes. isoelectric points of pancreatic cancer gamma-gtps varied in each case, but they were all higher than th ... | 1990 | 1967152 |
| evidence for projections from the rostral medullary raphe onto medullary catecholamine neurons in the rat. | following the iontophoretic deposition of phaseolus vulgaris leucoagglutinin (pha-l) into the rostral medullary raphe, which included portions of the caudal nucleus raphe magnus, rostral nucleus raphe pallidus, rostral nucleus raphe obscurus and rostral nucleus reticularis paragigantocellularis, two-color immunoperoxidase staining was employed to demonstrate contiguity between pha-l-immunoreactive (pha-li) varicose fibers and boutons and medullary catecholamine (ca) cells. raphe projections were ... | 1990 | 1968240 |
| efferent connections of the a1 noradrenergic cell group: a dbh immunohistochemical and pha-l anterograde tracing study. | immunohistochemical localization of the catecholamine biosynthetic enzymes tyrosine hydroxylase (th), dopamine beta-hydroxylase (dbh), and phenylethanolamine n-methyltransferase (pnmt) was employed to reveal the anatomical organization of the a1 noradrenergic cell group in the caudal ventrolateral medulla oblongata of the rat. subsequently, the supraspinal efferent axonal projections of a1 were investigated with a view to elucidating the anatomical substrates underlying its postulated function i ... | 1990 | 1976532 |
| expression of three plant glutamine synthetase cdna in escherichia coli. formation of catalytically active isoenzymes, and complementation of a glna mutant. | three cdna clones encoding the closely related glutamine synthetase (gs) alpha, beta and gamma polypeptides of phaseolus vulgaris (french bean) were recombinantly expressed in escherichia coli. the gs expression plasmids correctly synthesised the recombinant alpha, beta and gamma polypeptides which then assembled into catalytically active homo-octameric isoenzymes. these isoenzymes behaved similarly to their native homologues on ion-exchange and gel-filtration chromatography. furthermore, the al ... | 1990 | 1977583 |
| innervation of different peptide-containing neurons in the hippocampus by gabaergic septal afferents. | the termination pattern of septohippocampal axons visualized by anterograde transport of phaseolus vulgaris leucoagglutinin was studied in the hippocampal formation in the rat, with special reference to the innervation of neurons immunoreactive for the neuroactive peptides cholecystokinin, somatostatin or vasoactive intestinal polypeptide. the type i, gabaergic, septohippocampal afferents were shown to terminate on neurons immunoreactive for each of the three peptides. the cholecystokinin-like i ... | 1990 | 1978740 |
| sources of presumptive glutamatergic/aspartatergic afferents to the magnocellular basal forebrain in the rat. | the distribution of presumptive glutamatergic and/or aspartatergic neurons retrogradely labeled following injections of [3h]-d-aspartate into the magnocellular basal forebrain of the rat was compared with the distribution of neurons labeled by comparable injections of the nonspecific retrograde axonal tracer wheat germ agglutinin conjugated to horseradish peroxidase. cells retrogradely labeled by wheat germ agglutinin-horseradish peroxidase were found in a wide range of limbic and limbic-related ... | 1990 | 1982006 |
| tolerance to long-term feeding of isolated peanut lectin in the rat: evidence for a trophic effect on the small intestines. | previously we have shown that rats fed a diet containing raw peanut meal as the sole source of protein exhibited alterations in enzyme activity and composition of certain organs. to determine the effects of isolated peanut lectin on body growth and on the intestines, experiments were carried out in weanling, male, sprague-dawley rats fed a casein diet incorporated with purified peanut lectin at three levels, 0.004, 0.04, and 0.2% for 23 days. body weight gain was normal with all three diets. in ... | 1990 | 1982949 |
| expression of glutamine synthetase genes in roots and nodules of phaseolus vulgaris following changes in the ammonium supply and infection with various rhizobium mutants. | in this paper we have examined whether the four glutamine synthetase (gln) genes, expressed in roots and nodules of phaseolus vulgaris are substrate-inducible by ammonium. manipulation of the ammonium pool in roots, through addition and removal of exogenous ammonium, did not elicit any changes in the abundances of the four mrnas thus suggesting that the gln genes in roots of this legume are neither substrate-inducible by ammonium nor derepressed during nitrogen starvation. in nodules the effect ... | 1990 | 1983288 |
| targeting of glutamine synthetase to the mitochondria of transgenic tobacco. | two transgenic tobacco lines were genetically engineered to contain chimaeric genes encoding the glutamine synthetase (gs) gamma polypeptide of phaseolus vulgaris (french bean), expressed from the cauliflower mosaic virus 35s promoter. one (mit-1) contained two copies of a construct including the first 60 amino acids of the nicotiana plumbaginifolia beta-f1 atpase to target the gs polypeptide to the mitochondrion. the other (cyt-4) contained a single copy of a cytosolic gs construct. leaves of i ... | 1990 | 1983302 |
| decussation of bulbospinal respiratory axons at the level of the phrenic nuclei in adult rats: a possible substrate for the crossed phrenic phenomenon. | the axonal trajectories of inspiratory bulbospinal neurons were examined after deposition of the anterograde neuronal tracer phaseolus vulgaris leucoagglutinin (pha-l) into the rostral ventral respiratory group in rats. at the level of the phrenic nucleus, pha-l-labeled bulbospinal axons crossed the midline of the spinal cord in both the anterior gray and the anterior white commissure. these spinally decussating neurons provide a possible anatomical substrate for the respiratory reflex known as ... | 1991 | 1984430 |
| pseudomonas syringae pv. phaseolicola genomic clones harboring heterologous dna sequences suppress the same phaseolotoxin-deficient mutants. | cosmid cloning and mutagenesis were used to identify genes involved in the production of phaseolotoxin, the chlorosis-inducing phytotoxin of pseudomonas syringae pv. phaseolicola, the causal agent of halo blight of bean (phaseolus vulgaris l.). eight stable clones were isolated from a genomic cosmid library by en masse mating to 10 ethyl methanesulfonate (ems)-induced tox- mutants. in cross-matings, each suppressed all 10 mutants as well as an additional 70 ems-induced tox- mutants (and one uv-i ... | 1991 | 1991709 |
| restoration of thalamostriatal projections in rat neostriatal grafts: an electron microscopic analysis. | the thalamostriatal projections to rat neostriatal grafts were studied by using the phaseolus vulgaris-leucoagglutinin (pha-l) axonal tracing technique. two to 6 months after implantation of striatal primordia into adult neostriata, pha-l was injected into two different portions of the intralaminar nuclear complex of the thalamus. in the host neostriatum, labeled fibers from the parafascicular nucleus (pf) arborized in a large region in the neostriatum, but avoided small patchlike areas. most of ... | 1991 | 2005239 |
| the projection of locus coeruleus neurons to the spinal cord in the rat determined by anterograde tracing combined with immunocytochemistry. | pontospinal noradrenergic neurons located in the a5, a7 and locus coeruleus/subcoeruleus (lc/sc) nuclei are the major source of the noradrenergic innervation of the spinal cord. however, the specific terminations of spinally-projecting noradrenergic neurons located in these nuclei have not been clearly defined. the purpose of the experiments described in this report was to more precisely define the spinal terminations of neurons located in the lc/sc using the anterograde tracer phaseolus vulgari ... | 1991 | 2012966 |
| posteroventral cochlear nucleus projections to olivocochlear neurons. | the presence of ascending auditory inputs from the posteroventral cochlear nucleus (pvcn) to olivocochlear neurons was examined in guinea pig by using the combination phaseolus vulgaris-leucoagglutinin (pha-l) anterograde and horseradish peroxidase (hrp) retrograde tract-tracing technique. by labeling the somata of olivocochlear neurons after injection of hrp into the cochlea and simultaneously labeling terminal endings of pvcn efferent neurons after injection of pha-l into pvcn, we observed neu ... | 1991 | 2013640 |
| prefrontal cortical projections to the cholinergic neurons in the basal forebrain. | the prefrontal cortex (pfc) projections to the basal forebrain cholinergic cell groups in the medial septum (ms), vertical and horizontal limbs of the diagonal band of broca (vdb and hdb), and the magnocellular basal nucleus (mbn) in the rat were investigated by anterograde transport of phaseolus vulgaris leuco-agglutinin (pha-l) combined with acetylcholinesterase (ache) histochemistry or choline acetyltransferase (chat) immunocytochemistry. the experiments revealed rich pha-l-labeled projection ... | 1991 | 2013647 |
| starch bioavailability in the upper gastrointestinal tract of colectomized rats. | colectomized rats were used to compare the bioavailability of starch from canned peas, kidney beans, lima beans, corn, cooked rice and ain-76a purified diet. postoperative weight gains of colectomized and sham-operated rats were not different. test meals of approximately 2 and approximately 3.3 g (dry weight) of each food containing chromic oxide (0.6 g/100 g) were administered to 4-6 rats by gastric intubation. chromium recovery in ileal digesta 10 h after the meal ranged from 94 to 102%. signi ... | 1991 | 2019877 |
| positive and negative cis-acting dna domains are required for spatial and temporal regulation of gene expression by a seed storage protein promoter. | mutations affecting spatial and temporal regulation of a beta-phaseolin gene encoding the major storage protein of bean (phaseolus vulgaris) were analyzed by stable and transient transformation approaches. the results substantiate the value of transient assays for rapid determination of the functionality of cis-acting sequences and the importance of stable transformation to identify tissue-specific determinants. spatial information is specified primarily by two upstream activating sequences (uas ... | 1991 | 2026144 |
| spatial relationships of axons arising from the substantia nigra, spinal trigeminal nucleus, and pedunculopontine tegmental nucleus within the intermediate gray of the cat superior colliculus. | we have utilized two different anterograde transport methods (phaseolus vulgaris leucoagglutinin [pha-l] immunocytochemistry and autoradiography) in the same experiment to compare the sublaminar location and arrangement of tectopetal axons arising from the substantia nigra pars reticulata, the spinal trigeminal nucleus, and the pedunculopontine tegmental nucleus. our findings reveal that the nigrotectal projection terminates in a patchy fashion within three horizontally oriented sublaminae of th ... | 1991 | 2045535 |
| efferent projections from the lateral geniculate nucleus to the pineal complex of the mongolian gerbil (meriones unguiculatus). | the intergeniculate leaflet of the lateral geniculate nucleus is considered to modulate circadian activity rhythms probably mediated by a direct neuronal connection to the suprachiasmatic nucleus. the present study in the gerbil demonstrates, by anterograde tracing with phaseolus vulgaris-leucoaglutinin (pha-l), the existence of an additional neuronal projection from a subportion of the lateral geniculate nucleus, involving the intergeniculate leaflet, directly to the pineal gland. pha-l-immunor ... | 1991 | 2054848 |
| specificity in the projection patterns of accumbal core and shell in the rat. | the efferent projections of the core and shell areas of the nucleus accumbens were studied with a combination of anterograde and retrograde tract-tracing methods, including phaseolus vulgaris-leucoagglutinin, horseradish peroxidase and fluorescent tracers. both the core and shell regions project to pallidal areas, i.e. ventral pallidum and entopeduncular nucleus, with a distinct topography in the sense that the core projection is located in the dorsolateral part of ventral pallidum, whereas the ... | 1991 | 2057066 |
| projections from the rostral ventrolateral medulla to brainstem monoamine neurons in the rat. | following the iontophoretic deposition of phaseolus vulgaris leucoagglutinin (pha-l) into the rostral ventrolateral medulla (rvl), two-color immunoperoxidase staining was employed to demonstrate contiguity between pha-l-immunoreactive (pha-li) varicose fibers and boutons and brainstem monoaminergic cells. black-stained pha-li cells in the deposition site were found to be located among amber-stained phenylethanolamine n-methyl transferase-immunoreactive (pnmt-i) neurons of the c1 cell group. rvl ... | 1991 | 2057140 |
| projections from the rostral parvocellular reticular formation to pontine and medullary nuclei in the rat: involvement in autonomic regulation and orofacial motor control. | the efferent connections of the rostral parvocellular reticular formation to pontine and medullary nuclei in the rat were studied with anterogradely transported phaseolus vulgaris leucoagglutinin. dense innervations from the rostral parvocellular reticular formation were found in the mesencephalic trigeminal nucleus, the supratrigeminal area, the motor trigeminal nucleus, the motor trigeminal nucleus, the facial, hypoglossal and parabrachial nuclei and specific parts of the caudal parvocellular ... | 1991 | 2062440 |
| cytochrome p450 ia2 activity in man measured by caffeine metabolism: effect of smoking, broccoli and exercise. | the activity of 3 enzymes related to the bioactivation of toxic compounds and the development of cancer--cytochrome p450 ia2, n-acetyl transferase (nat), and xanthine oxidase (xo)--can be measured from the ratios of formed metabolites excreted into urine. in the 3 experiments that comprised this study, subjects received at least 1 cup of coffee 2- 6 hours before spot urine samples were taken. the subjects included 335 healthy male and female volunteers who provided information on tobacco, caff ... | 1991 | 2069014 |
| organization of ascending hypothalamic projections to the rostral forebrain with special reference to the innervation of cholinergic projection neurons. | axonal projections from hypothalamic nuclei to the basal forebrain, and their relation to cholinergic projection neurons in particular, were studied in the rat by using the anterograde tracer phaseolus vulgaris-leucoagglutinin (pha-l) in combination with choline acetyltransferase (chat) immunocytochemistry. discrete iontophoretic pha-l injections were delivered to different portions of the caudal lateral hypothalamus, as well as to various medial hypothalamic areas, including the ventromedial, d ... | 1991 | 2071698 |
| selenium as a novel regulator of porphyrin biosynthesis in germinating seedlings of mung bean (phaseolus vulgaris). | 5-aminolevulinic acid, porphyrin and chlorophyll contents as well the activities of 5-aminolevulinic acid dehydratase and pbg deaminase were studied in selenium treated mung bean seedlings. selenium had no effect on 5-aminolevulinic acid synthetic ability but inhibited 5-aminolevulinic acid dehydratase and pbg deaminase activities. further, it was observed that selenium induced accumulation of protoporphyrin-ix and mg-protoporphyrin ester and decreased chlorophyll levels in both light and dark-g ... | 1990 | 2076102 |
| organization of local axon collaterals of efferent projection neurons in rat visual cortex. | we have studied the laminar origins of local long-range connections within rat primary visual cortex (area 17), by using retrograde tracing of nerve cell bodies with fluorescent markers. injections throughout the thickness of cortex produce distinct laminar labeling patterns which indicate that a substantial number of cells in layers 2/3, 5, and 6 have wide local axon collateral arbors, while the local arbors of layer 4 cells are much narrower. double labeling experiments which combined area 17 ... | 1990 | 2081821 |
| identification and cloning of nodulation genes and host specificity determinants of the broad host-range rhizobium leguminosarum biovar phaseoli strain ciat899. | rhizobium leguminosarum biovar phaseoli type ii strain ciat899 nodulates a wide range of hosts: phaseolus vulgaris (beans), leucaena esculenta (leucaena) and macroptilium atropurpureum (siratro). a nodulation region from the symbiotic plasmid has been isolated and characterized. this region, which is contained in the overlapping cosmid clones pcv38 and pcv117, is able to induce nodules in beans, leucaena and siratro roots when introduced in strains cured for the symbiotic plasmid, psym. in addit ... | 1990 | 2082147 |
| evidence for interconnections between the two segments of the globus pallidus in primates: a pha-l anterograde tracing study. | small injections of the lectin phaseolus vulgaris-leucoagglutinin (pha-l) in the external segment of the pallidum (gpe) in the squirrel monkey (saimiri sciureus) and in the rhesus monkey (macaca mulatta) led to anterograde labeling of fibers in the internal segment of the pallidum (gpi). these fibers formed numerous large varicosities reminiscent of terminal boutons, which closely surrounded gpi cell bodies and primary dendrites. conversely, pha-l injections in the gpi of squirrel monkeys produc ... | 1990 | 2085730 |
| multiplicity of vestibulospinal projections to the upper cervical spinal cord of the cat: a study with the anterograde tracer phaseolus vulgaris leucoagglutinin. | the distribution and frequency of vestibulospinal axons and boutons in the upper cervical spinal cord of the cat were investigated. the anterograde tracer phaseolus vulgaris leucoagglutinin (pha-l) was injected into discrete regions of the vestibular nuclei, including the medial and descending nuclei, as well as small regions of the lateral vestibular nucleus along its medial border with the medial vestibular nucleus. in contrast to previous reports, labelled vestibulospinal axons were not found ... | 1990 | 2086608 |
| polyamines in intestinal growth. | the total cellular mass of the small intestine is well controlled and can adapt, with hypo- or hyperplasia, to a wide variety of stimuli. luminal nutrients, hormonal factors and pancreatic and biliary secretions have all been implicated in the regulation of intestinal mucosal growth. the polyamines (putrescine, spermidine and spermine) and the key enzyme controlling their synthesis (ornithine decarboxylase. odc) are critical for many cell growth processes and appear to play important roles in in ... | 1990 | 2088816 |
| interaction of lectins with yersinia pestis strains. | the ability of lectins to interact with yersinia pestis strains isolated from rodent fleas and human biological fluids, obtained from different geographic areas, was examined. lectins of canavalia ensiformis, ulex europaeus, phaseolus vulgaris, and triticum vulgaris, as well as a new autochthonous lectin of swartzia pickellii of undefined specificity, were used. most of the y. pestis strains did not agglutinate with u. europaeus or c. ensiformis lectin. however, p. vulgaris lectin agglutinated s ... | 1990 | 2091525 |
| characterization of galactose-containing glycoconjugates in the human retina: a lectin histochemical study. | seven specimens of morphologically normal formalin-fixed and paraffin-embedded human retina were studied using a panel of fourteen biotinylated lectins, all of which react with glycoconjugates containing galactose and n-acetylgalactosamine residues. agaricus bisporus (aba), bauhinia purpurea (bpa), phaseolus vulgaris (pha-e), peanut (pna) and ricinus communis (rca-i) agglutinins labeled photoreceptor cells prior to enzymatic predigestion. bpa and pna bound specifically to cones. the plexiform la ... | 1990 | 2091899 |
| parietal projection of thalamocortical fibers from the ventroanterior-ventrolateral complex of the cat thalamus. | anterograde labelling following focal injections of phaseolus vulgaris leucoagglutinin was used to identify the parietal distribution of thalamocortical (tc) fibers from the ventroanterior-ventrolateral (va-vl) complex of the cat thalamus. in injections in the ventrolateral or the caudal part of the va-vl complex, labelled tc fibers were distributed in layers i, iii and iv of the parietal areas 5a and/or 5b, whereas in injections located more rostrally or dorsomedially, labelled tc fibers were a ... | 1990 | 2094818 |
| liver and kidney nitrogen uptake in rats fed beans enriched with 15nitrogen through (15nh4)2so4 used as soil fertilizer. | this study was conducted to determine the incorporation of 15nitrogen (15n) into liver and kidney of a group of rats (n = 17) fed a mixture of cooked rice and beans for 4 weeks. the beans (phaseolus vulgaris), grown in soil cultivated with (15nh4)2so4, had 1.5% 15n enrichment. three or four rats were sacrificed weekly and a control group (n = 13) received an isonitrogen and isocaloric reference casein diet. the amount of nitrogen transferred from the beans to the liver at the end of the first, s ... | 1990 | 2101091 |
| luminal and basolateral polyamine uptake by rat small intestine stimulated to grow by phaseolus vulgaris lectin phytohaemagglutinin in vivo. | luminal and basolateral uptake of polyamines by the rat small intestine was studied in vivo. in the concentration range studied (0.1-5 mg per rat) 23-47% of the individual polyamines given intragastrically were found in the body after 1 h, with the small intestine retaining 4-12% of the dose. with spermidine or spermine, labelled polyamines accounted for 85-96% of the counts in the small intestine and between 72-82% were in the form given. however, with putrescine only 29-39% of the label found ... | 1990 | 2109637 |
| cloning and expression of rat histidase. homology to two bacterial histidases and four phenylalanine ammonia-lyases. | histidase (histidine ammonia-lyase, ec 4.3.1.3) catalyzes the deamination of histidine to urocanic acid. apart from phenylalanine ammonia-lyase, which is not expressed in animals, histidase is the only enzyme known to have a dehydroalanine residue in its active site. the amino site precursor and the mechanism of formation of dehydroalanine are not known. as an initial step to determining the precursor of dehydroalanine in histidase, we have isolated a functional cdna clone for histidase from a r ... | 1990 | 2120224 |
| the effect of the consumption of red kidney beans (phaseolus vulgaris) on the growth of rats and the implications for human populations. | raw red kidney beans (phaseolus vulgaris) at a level as low as 1% of diet have been shown to cause death in rats in two weeks. beans cooked at 100 degrees for 30 mins, and incorporated at a level as high as 20% of diet do not retard growth when tested against casein. however, when beans which have cooked at 70 degrees for 30 mins are incorporated growth retardation is almost as great as that which occurs when raw beans are fed. the small amount of lectin present in beans cooked at 70 degrees mig ... | 1990 | 2126293 |
| fetal striatal neurons grafted into the ibotenate lesioned adult striatum: efferent projections and synaptic contacts in the host globus pallidus. | the efferent projections to the host brain from intrastriatal grafts have been examined at the ultrastructural level. cell suspensions of e14 rat fetal striatal tissue were implanted into the ibotenic acid lesioned caudate-putamen of adult rats. after survival times of at least five months, the anterograde neuronal tracer phaseolus vulgaris leucoagglutinin was injected into the grafts. consistent with our previous light microscopical analysis, anterogradely labelled fibres could be followed from ... | 1990 | 2133346 |
| thalamic origin of neuropeptide y innervation of the accessory optic nucleus of the pigeon (columba livia). | immunohistochemical and tracing techniques were used in combination to reveal the source of a neuropeptide y-like immunoreactive (npy-li) plexus in the nucleus of the basal optic root (nbor) of the pigeon accessory optic system. injections of rhodamine-labeled latex microspheres into nbor produced retrograde labeling of a population of neurons interposed between the principal optic nucleus of the dorsolateral thalamus (equivalent to the mammalian dorsal lateral geniculate nucleus) and the ventra ... | 1990 | 2134848 |
| expression analysis of a pseudogene in transgenic tobacco: a frameshift mutation prevents mrna accumulation. | seeds of the pinto cultivar of the common bean, phaseolus vulgaris, are deficient in phytohemagglutinin (pha), a lectin normally composed of two different polypeptides (pha-e and pha-l). in pinto seeds, there is no pha-e and only small amounts of pha-l. the gene coding for the pinto pha-e, pdlec1, is a pseudogene as a result of a single base pair deletion in codon 11, causing a frameshift and premature termination of translation. this mutation explains the absence of the pha-e polypeptide but no ... | 1990 | 2152115 |
| a short domain of the plant vacuolar protein phytohemagglutinin targets invertase to the yeast vacuole. | phytohemagglutinin (pha), the seed lectin of the common bean, accumulates in protein storage vacuoles of storage parenchyma cells in cotyledons. when expressed in yeast, pha is efficiently targeted to the yeast vacuole [tague and chrispeels (1987). j. cell biol. 105, 1971-1979]. to identify vacuolar sorting information in pha, a series of 3' deletions of the pha gene were fused in-frame to a truncated yeast invertase gene. an amino-terminal portion of pha composed of a 20-residue signal sequence ... | 1990 | 2152175 |
| fermentation of vegetable fiber in the intestinal tract of rats and effects on fecal bulking and bile acid excretion. | the fermentative breakdown and fecal bulking capacity of dietary fiber from various vegetables (carrots, rutabagas, peas, green beans and brussels sprouts) were evaluated in balance experiments in rats. the excretion of bile acids, protein and fat was also studied. the addition of blanched vegetables providing 10 g of fiber/100 g of diet caused a two-(rutabagas) to threefold (carrots) increase in fecal dry weight compared to that with a basal fiber-free diet. with green beans and brussels sprout ... | 1990 | 2160526 |
| organization of the thalamostriatal projections in the rat, with special emphasis on the ventral striatum. | the organization of the thalamic projections to the ventral striatum in the rat was studied by placing injections of the retrograde tracer cholera toxin subunit b in the ventral striatum and small deposits of the anterograde tracer phaseolus vulgaris-leucoagglutinin (pha-l) in individual midline and intralaminar thalamic nuclei. in order to provide a complete map of the midline and intralaminar thalamostriatal projections, pha-l injections were also made in those parts of the intralaminar nuclei ... | 1990 | 2172326 |
| postnatal development of crossed and uncrossed corticorubral projections in kitten: a pha-l study. | morphological changes in individual corticorubral fibers and the pattern of crossed and uncrossed corticorubral projections were studied during the postnatal development of cats in order to understand cellular mechanisms for restriction of corticorubral projections with development. the anterograde tracer phaseolus vulgaris leucoagglutinin (pha-l) was injected into restricted areas of the pericruciate cortex in kittens and pha-l-labeled axons in the red nucleus were examined at postnatal days (p ... | 1990 | 2172328 |
| combination of anterograde tracing with phaseolus vulgaris-leucoagglutinin, retrograde fluorescent tracing and fixed-slice intracellular injection of lucifer yellow. | a method is described for visualizing, in one histological section, three successive components of a putative neuronal network in the central nervous system: (i) fibres from neurones in an area a that innervate (ii) dendrites of morphologically identified neurones in an area b projecting (iii) to a specific target area c. the tracer phaseolus vulgaris-leucoagglutinin (pha-l) is injected in area a in order to anterogradely label fibres and terminal varicosities in area b. in the same surgical ses ... | 1990 | 2172663 |
| efferent projections from the subnucleus reticularis dorsalis (srd): a phaseolus vulgaris leucoagglutinin study in the rat. | small iontophoretic applications of phaseolus vulgaris leucoagglutinin (pha-l) were used in rats to study the efferent projections from a restricted region in the reticular formation of the caudal medulla: the subnucleus reticularis dorsalis (srd). it was found that fibres from srd project mainly to the spinal cord, the oral motor nuclei, the dorsal accessory inferior olive, the giganto- and parvo-cellular nuclei of the reticular formation and the parafascicular and ventromedian nuclei of the th ... | 1990 | 2173815 |
| distribution and synaptic contacts of the cortical terminals arising from neurons in the rat ventromedial thalamic nucleus. | injections of the anterograde tracer phaseolus vulgaris-leucoagglutinin within the ventromedial thalamic nucleus resulted in many filled fibres in the frontal areas of rat cerebral cortex. the fibres were restricted to the upper part of layer i except in a small area of motor cortex where terminals were also found in deeper layers. terminals were also seen in the striatum, in parts of the mesencephalic reticular formation and occasionally in the contralateral ventromedial nucleus. there is some ... | 1990 | 2175021 |
| organization of individual cortical axons projecting from area v1 (area 17) to v2 (area 18) in the macaque monkey. | the present study uses the anterograde tracer, phaseolus vulgaris-leucoagglutinin (pha-l), to investigate the detailed morphology of individual axons projecting from area v1 to prestriate area v2. observations are derived from serial reconstructions of 45 axons. axons are found to differ both in laminar distribution and in arbor size. the majority (25/45; 56%) terminate in the upper half of layer 4 and the lower part of layer 3. terminal clusters typically measure about 200 microns in diameter ( ... | 1990 | 2176095 |
| the in situ detectability of the neuroanatomical tracer phaseolus vulgaris-leucoagglutinin (pha-l). | we determined the in situ detectability of the neuroanatomical tracer phaseolus vulgaris-leucoagglutinin (pha-l). the tracer was bilaterally injected in the nucleus accumbens of the ventral striatum of young adult rats. after post-injection survival periods ranging from 4.5 to 16 weeks, sections of the brain were subjected to immunocytochemical detection of the transported pha-l. we examined the labelled neurons in the injection sites, the labelled local and efferent fibres, and the labelled ter ... | 1990 | 2180431 |
| solution conformation of several free trnaleu species from bean, yeast and escherichia coli and interaction of these trnas with bean cytoplasmic leucyl-trna synthetase. a phosphate alkylation study with ethylnitrosourea. | the solution conformation of eight leucine trnas from phaseolus vulgaris, baker's yeast and escherichia coli, characterized by long variable regions, and the interaction of four of them with bean cytoplasmic leucyl-trna synthetase were studied by phosphate mapping with ethylnitrosourea. phosphate reactivities in the variable regions agree with the existence of rna helices closed by miniloops. at the junction of these regions with the t-stem, phosphate 48 is strongly protected, in contrast to sma ... | 1990 | 2187177 |
| cerebellar nucleo-olivary projections in the rat: an anterograde tracing study with phaseolus vulgaris-leucoagglutinin (pha-l). | in order to evaluate the reciprocity of olivo-cerebellar and cerebello-olivary connections, a detailed description of the cerebellar nucleo-olivary projection in the rat is presented using small, iontophoretic injections of the anterograde tracer phaseolus vulgaris-leucoagglutinin. sparse projections were found to arise from the rostral part of the medial cerebellar nucleus toward the lateral part of the caudal medial accessory olive. its medial parts receive a projection from the dorsolateral p ... | 1990 | 2212106 |
| direct projections from the anterior and tuberal regions of the lateral hypothalamus to the rostral part of the pineal complex of the rat. an anterograde neuron-tracing study by using phaseolus vulgaris leucoagglutinin. | the anterograde tracer, phaseolus vulgaris leucoagglutinin, was injected into the lateral hypothalamic division of the wistar rat. after 7 days of survival the animals were fixed by perfusion, and cryostat sections processed for visualization of the tracer by immunohistochemistry. injections into the anterior and tuberal regions of the lateral hypothalamic division labeled neurons which projected caudally to the rostral part of the pineal complex, e.g. the deep pineal gland and the pineal stalk. ... | 1990 | 2224533 |
| pathologic changes of the small intestinal mucosa of pigs after feeding phaseolus vulgaris beans. | the jejunal mucosa of pigs fed diets containing phaseolus vulgaris beans was characterized grossly as mucosal atrophy and microscopically as atrophy and blunting of the villus in association with elongation of crypts with cells with increased mitotic activity. these morphologic findings were most severe in the proximal and middle parts of the jejunum. compared to controls, goblet cells were significantly decreased in the villus but markedly increased in the crypt region. the activity of aminopep ... | 1990 | 2238385 |
| increase of beta 1-6-branched oligosaccharides in human esophageal carcinomas invasive against surrounding tissue in vivo and in vitro. | the -glcnac beta 1-6man- (beta 1-6) branched n-glycosidic oligosaccharides expressed on tumor cells have been found to contribute to malignant and metastatic potential in experimental tumor models. phaseolus vulgaris leukoagglutinin (l-pha) requires the beta 1-6-linked lactosamine antenna for high-affinity binding and was used histochemically to characterize the distribution of these sugar structures in human esophageal squamous cell carcinomas from 42 patients. leukoagglutinin-reactive carcinom ... | 1990 | 2240156 |
| the rat olivocerebellar system visualized in detail with anterograde pha-l tracing technique, and sprouting of climbing fibers demonstrated after subtotal olivary lesions. | the rat olivocerebellar climbing fiber system has been investigated at the light and electron microscopic level with anterograde phaseolus vulgaris leucoagglutinin (pha-l) tracing. from pha-l injections in different parts of the inferior olive labelled axons could be traced to the contralateral cerebellum. arriving in the deep cerebellar white matter, the olivocerebellar axons ran around and through the cerebellar nuclei. plexuses of labelled terminal fibers appeared in the cerebellar nuclei, an ... | 1990 | 2245134 |
| three-peaked chemiluminescent response of human peripheral blood leukocytes following stimulation with phytohaemagglutinin (pha). | luminol-enhanced chemiluminescence (cl) was used to examine the response of various leukocyte populations following stimulation with a crude extract of phaseolus vulgaris, namely phytohaemagglutinin (pha-c). populations stimulated included a human peripheral mixed leukocyte preparation (mlp), and purified preparations of lymphocytes, monocytes and polymorphonuclear leukocytes (pmnl). mouse peritoneal exudate cells and the lymphocytic cells lines molt #4 and daudi were also stimulated. following ... | 1990 | 2251938 |
| ontogenesis of the secretory epithelium of the bovine subcommissural organ. a histofluorescence study using lectins and monoclonal antibodies. | a spatio-temporal analysis of the differentiation of a group of specialized (secretory) ependymal cells in the subcommissural organ (sco) of the brain was undertaken in the bovine using a monoclonal antibody (c1b8a8) which is specific of the secretory process in this organ. in addition, lectins (concanavalin agglutinin (con a), lens culinaris agglutinin (lca), wheat germ agglutinin (wga), and phaseolus vulgaris agglutinin (pha] were used to analyse the maturation of the carbohydrate moieties of ... | 1990 | 2253320 |
| relationship between survival and binding of plant lectins during small intestinal passage and their effectiveness as growth factors. | the effects on the small intestine and the growth of rats of six pure plant lectins: pha (phaseolus vulgaris); sbl (glycine maxima); sna-i and sna-ii (sambucus nigra); gna (galanthus nivalis) and vfl (vicia faba), covering most sugar specificities found in nature, were studied in vivo. variable amounts, 25% (vfl) to 100% (pha, gna) of the lectins administered intragastrically, remained in immunochemically intact form in the small intestine after 1 h. all lectins, except gna, showed binding to th ... | 1990 | 2262064 |
| polyamine metabolism and uptake during phaseolus vulgaris lectin, pha-induced growth of rat small intestine. | kidney bean lectin, pha, stimulated the hyperplastic and hypertrophic growth of rat small intestine. this growth was preceded by a rapid accumulation of polyamines in the small intestine. however, since the lectin had little effect on in situ polyamine biosynthesis, most of the polyamines must have been of extracellular origin. to investigate the source of polyamines, both the luminal uptake and basolateral transport of polyamines by the rat small intestine were measured in vivo. luminal polyami ... | 1990 | 2262068 |
| comparison of growth, nitrogen metabolism and organ weights in piglets and rats fed on diets containing phaseolus vulgaris beans. | the effects of lectins in the diet have been mainly studied in rats. an important question is whether results obtained in rats can be extrapolated to larger animals like the pig. phaseolus vulgaris beans are rich in toxic lectins. therefore a study was carried out to compare the effects of diets containing 200 g phaseolus vulgaris beans (raw or toasted)/kg in rats and piglets. live-weight gain, nitrogen digestibility and n balance were much lower in piglets than in rats fed on diets containing r ... | 1990 | 2265182 |
| effect of variable protein contents in diets containing phaseolus vulgaris beans on performance, organ weights and blood variables in piglets, rats and chickens. | a comparison was made of the effects of antinutritional factors present in phaseolus vulgaris on piglets, rats and chickens. also the hypothesis of whether the negative effect on weight gain due to the inclusion of raw phaseolus vulgaris in the diet can be attributed to an insufficient supply of amino acids was tested. test diets containing 200 g raw phaseolus beans/kg were balanced for digestible protein and amino acids; in one diet extra casein was incorporated. the main response criteria were ... | 1990 | 2265183 |
| intersubnuclear connections within the rat trigeminal brainstem complex. | prior intracellular recording and labeling experiments have documented local-circuit and projection neurons in the spinal trigeminal (v) nucleus with axons that arborize in more rostral and caudal spinal trigeminal subnuclei and nucleus principalis. anterograde tracing studies were therefore carried out to assess the origin, extent, distribution, and morphology of such intersubnuclear axons in the rat trigeminal brainstem nuclear complex (tbnc). phaseolus vulgaris leucoagglutinin (pha-l) was use ... | 1990 | 2291376 |
| descending projections from the hypothalamic paraventricular nucleus to the a5 area, including the superior salivatory nucleus, in the rat. | the descending projection of the hypothalamic paraventricular nucleus (pvn) to the a5 area was elucidated using a technique that combines retrograde labeling with horseradish peroxidase (hrp), anterograde labeling with pha-l (phaseolus vulgaris leucoagglutinin and immunohistochemistry for dopamine-beta-hydroxylase (dbh). following an iontophoretic injection of pha-l into the pvn, hrp was applied to the greater petrosal nerve. frozen sections of the hypothalamus and the caudal pons were first tre ... | 1990 | 2292271 |