| adaptive evolution of rhizobial symbiotic compatibility mediated by co-evolved insertion sequences. | mutualism between bacteria and eukaryotes has essential roles in the history of life, but the evolution of their compatibility is poorly understood. here we show that different sinorhizobium strains can form either nitrogen-fixing nodules or uninfected pseudonodules on certain cultivated soybeans, while being all effective microsymbionts of some wild soybeans. however, a few well-infected nodules can be found on a commercial soybean using inocula containing a mixed pool of tn5 insertion mutants ... | 2018 | 28800133 |
| rna-seq study reveals genetic responses of diverse wild soybean accessions to increased ozone levels. | ozone is an air pollutant widely known to cause a decrease in productivity in many plant species, including soybean (glycine max (l.) merr). while the response of cultivated soybean to ozone has been studied, very little information is available regarding the ozone response of its wild relatives. | 2017 | 28662633 |
| changes in gene expression between a soybean f1 hybrid and its parents are associated with agronomically valuable traits. | soybean [glycine max (l.) merr.] genetic diversity is limited because domesticated soybean has undergone multiple genetic bottlenecks. its progenitor, the wild soybean [glycine soja siebold & zucc], has not undergone the same intense selection and is much more genetically diverse than domesticated soybean. however, the agronomic importance of diversity in wild soybean is unclear, and its weedy nature makes assessment difficult. to address this issue, we chose for study a highly selected, adapted ... | 2017 | 28493991 |
| rna-seq data comparisons of wild soybean genotypes in response to soybean cyst nematode (heterodera glycines). | soybean [glycine max (l.) merr.] is an important crop rich in vegetable protein and oil, and is a staple food for human and animals worldwide. however, soybean plants have been challenged by soybean cyst nematode (scn, heterodera glycines), one of the most damaging pests found in soybean fields. applying scn-resistant cultivars is the most efficient and environmentally friendly strategy to manage scn. currently, soybean breeding and further improvement in soybean agriculture are hindered by seve ... | 2017 | 28856099 |
| comparative rna-seq analysis uncovers a complex regulatory network for soybean cyst nematode resistance in wild soybean (glycine soja). | soybean cyst nematode (scn) is the most damaging pest of soybean worldwide. the molecular mechanism of scn resistance remains largely unknown. we conducted a global rna-seq comparison between a resistant genotype (s54) and a susceptible genotype (s67) of glycine soja, the wild progenitor of soybean, to understand its regulatory network in scn defense. the number of differentially expressed genes (degs) in s54 (2,290) was much larger than that in s67 (555). a number of defense-related genes/pathw ... | 2017 | 28852059 |
| genetic architecture of wild soybean (glycine soja) response to soybean cyst nematode (heterodera glycines). | the soybean cyst nematode (scn) is one of the most destructive pathogens of soybean plants worldwide. host-plant resistance is an environmentally friendly method to mitigate scn damage. to date, the resistant soybean cultivars harbor limited genetic variation, and some are losing resistance. thus, a better understanding of the genetic mechanisms of the scn resistance, as well as developing diverse resistant soybean cultivars, is urgently needed. in this study, a genome-wide association study (gw ... | 2017 | 28710561 |
| genome-wide identification of major intrinsic proteins in glycine soja and characterization of gmtip2;1 function under salt and water stress. | in different plant species, aquaporins (aqps) facilitate water movement by regulating root hydraulic conductivity under diverse stress conditions such as salt and water stresses. to improve survival and yield of crop plants, a detailed understanding of stress responses is imperative and required. we used glycine soja genome as a tool to study aqps, considering it shows abundant genetic diversity and higher salt environment tolerance features and identified 62 gs aqp genes. additionally, this stu ... | 2017 | 28646139 |
| hematologic complications in a patient with glycine soja polyagglutination following fresh frozen plasma transfusion. | polyagglutination is a rare and underdiagnosed condition, characterized by agglutination of red blood cells(rbcs) with almost all abo-compatible adult sera. polyagglutination can occur when a cryptantigen is exposed on rbcs via microbial enzyme activity. becausenearly all adults naturally produce antibodies against cryptantigens, transfusion of plasma can cause unexpected hemolysis and hematologic complications, such as thrombocytopenia and disseminated intravascular coagulation, in patients who ... | 2017 | 28657762 |
| a soybean quantitative trait locus that promotes flowering under long days is identified as ft5a, a flowering locus t ortholog. | flowering locus t (ft) is an important floral integrator whose functions are conserved across plant species. in soybean, two orthologs, ft2a and ft5a, play a major role in initiating flowering. their expression in response to different photoperiods is controlled by allelic combinations at the maturity loci e1 to e4, generating variation in flowering time among cultivars. we determined the molecular basis of a quantitative trait locus (qtl) for flowering time in linkage group j (chromosome 16). f ... | 2016 | 27422993 |
| differential expression of a wrky gene between wild and cultivated soybeans correlates to seed size. | soybean (glycine max) probably originated from the wild soybean (glycine soja). glycine max has a significantly larger seed size, but the underlying genomic changes are largely unknown. candidate regulatory genes were preliminarily proposed by data co-localizing rna sequencing with the quantitative loci (qtls) for seed size. the soybean gene locus soywrky15a and its orthologous genes from g. max (gmwrky15a) and g. soja (gswrky15a) were analyzed in detail. the coding sequences were nearly identic ... | 0 | 28472462 |
| fingerprinting soybean germplasm and its utility in genomic research. | the united states department of agriculture, soybean germplasm collection includes 18,480 domesticated soybean and 1168 wild soybean accessions introduced from 84 countries or developed in the united states. this collection was genotyped with the soysnp50k beadchip containing greater than 50k single-nucleotide polymorphisms. redundant accessions were identified in the collection, and distinct genetic backgrounds of soybean from different geographic origins were observed that could be a unique re ... | 2015 | 26224783 |
| functional characterization of naturally occurring wild soybean mutant (sg-5) lacking astringent saponins using whole genome sequencing approach. | triterpenoid saponins are one of the most highly accumulated groups of functional components in soybean (glycine max) and the oxidative reactions during their biosynthesis are required for their aglycone diversity. natural mutants of soyasaponins in wild soybean (glycine soja) are valuable resources for establishing the soyasaponin biosynthesis pathway and breeding new soybean varieties. in this study, we investigated the genetic mechanism behind the absence of group a saponins in a korean wild ... | 2018 | 29362093 |
| heterosis and combining ability estimates in isoflavone content using different parental soybean accessions: wild soybean, a valuable germplasm for soybean breeding. | isoflavone, a group of secondary metabolites in soybean, is beneficial to human health. improving isoflavone content in soybean seeds has become one of the most important breeding objectives. however, the narrow genetic base of soybean cultivars hampered crop improvement. wild soybean is an extraordinarily important gene pool for soybean breeding. in order to select an optimal germplasm for breeding programs to increase isoflavone concentration, 36 f1 soybean progenies from different parental ac ... | 2015 | 25607952 |
| gssnap33, a novel glycine soja snap25-type protein gene: improvement of plant salt and drought tolerances in transgenic arabidopsis thaliana. | the n-ethylmaleimide-sensitive factor attachment protein receptor (snare) superfamily, specifically the snap25-type proteins and t-snares, have been proposed to regulate cellular processes and plant resistance mechanisms. however, little is known about the role of snap25-type proteins in combating abiotic stresses, specifically in wild soybean. in the current study, the isolation and functional characterization of the putative synaptosomal-associated snap25-type protein gene gssnap33 from wild s ... | 2017 | 28841544 |
| elevation of soybean seed oil content through selection for seed coat shininess. | many leguminous species have adapted their seed coat with a layer of powdery bloom that contains hazardous allergens and makes the seeds less visible, offering duel protection against potential predators 1 . nevertheless, a shiny seed surface without bloom is desirable for human consumption and health, and is targeted for selection under domestication. here we show that seed coat bloom in wild soybeans is mainly controlled by bloom1 (b1), which encodes a transmembrane transporter-like protein fo ... | 2018 | 29292374 |
| genetic and chemical analysis of a key biosynthetic step for soyasapogenol a, an aglycone of group a saponins that influence soymilk flavor. | although certain saponins in soybean seeds have been reported to have health benefits, group a acetyl saponins cause undesirable bitter and astringent tastes in soy products. therefore, reduction or elimination of group a saponins is an important target for soybean breeders. a wide survey of cultivated and wild soybean germplasm identified a mutant line that lacked group a saponins. the absence of soyasapogenol a, a group a saponin aglycone, is controlled by a single recessive allele, sg-5 that ... | 2013 | 23229125 |
| qtl mapping of antixenosis resistance to common cutworm (spodoptera litura fabricius) in wild soybean (glycine soja). | the common cutworm (ccw; spodoptera litura fabricius) is a serious herbivorous insect pest of soybean (glycine max) in asia and oceania. previously, we identified quantitative trait loci (qtls) for ccw-antibiosis-resistance, ccw-1 and ccw-2, and antixenosis-resistance, qrslx1 and qrslx2, in the cultivar 'himeshirazu'. the effects of these qtls are useful in the breeding of ccw-resistant cultivars. in this study, we conducted an antixenosis bioassay on ccw using recombinant inbred lines derived f ... | 2017 | 29232719 |
| chromosome segment detection for seed size and shape traits using an improved population of wild soybean chromosome segment substitution lines. | size and shape of soybean seeds are closely related to seed yield and market value. annual wild soybeans have the potential to improve cultivated soybeans, but their inferior seed characteristics should be excluded. to detect quantitative trait loci (qtls)/segments of seed size and shape traits in annual wild soybean, its chromosome segment substitution lines (cssls) derived from nn1138-2 (recurrent parent, glycine max) and n24852 (donor parent, glycine soja) and then modified 2 iterations (code ... | 2017 | 29158636 |
| soybean mosaic virus: a successful potyvirus with a wide distribution but restricted natural host range. | taxonomy. soybean mosaic virus (smv) is a species within the genus potyvirus, family potyviridae that includes almost a quarter of all known plant rna viruses affecting agriculturally important plants. the potyvirus genus is the largest of all genera of plant rna viruses with 160 species. particle. the filamentous particles of smv, typical of potyviruses, are about 7,500 å long and 120 å in diameter with a central hole of about 15 å in diameter. coat protein residues are arranged in helice of ab ... | 2017 | 29134790 |
| likelihood assessment for gene flow of transgenes from imported genetically modified soybean (glycine max (l.) merr.) to wild soybean (glycine soja seib. et zucc.) in japan as a component of environmental risk assessment. | environmental risk assessment is required for genetically modified (gm) crops before their import into japan. annual roadside monitoring along transportation routes from ports to processing facilities for gm soybean (glycine max (l.) merr.) have been requested as a condition of import only approval because of lack of information on the likelihood of persistence of imported gm soybean for food, feed and processing and the potential for transfer of transgenes into wild soybean (glycine soja seib. ... | 2017 | 29085244 |
| molecular cloning, recombinant expression and antifungal activity of bncpi, a cystatin in ramie (boehmeria nivea l.). | phytocystatins play multiple roles in plant growth, development and resistance to pests and other environmental stresses. a ramie (boehmeria nivea l.) phytocystatin gene, designated as bncpi, was isolated from a ramie cdna library and its full-length cdna was obtained by rapid amplification of cdna ends (race). the full-length cdna sequence (691 bp) consisted of a 303 bp open reading frame (orf) encoding a protein of 100 amino acids with deduced molecular mass of 11.06 kda and a theoretical isoe ... | 2017 | 29019965 |
| transcriptomic variation in proanthocyanidin biosynthesis pathway genes in soybean (glycine spp.). | proanthocyanidins are oligomeric or polymeric end products of flavonoid metabolic pathways starting with the central phenylpropanoid pathway. although soybean (glycine spp.) seeds represent a major source of nutrients for the human diet, as well as components for the cosmetics industry as a result of their high levels of flavonoid metabolites, including isoflavonoids, anthocyanins and proanthocyanidins, the genetic regulatory mechanisms underlying proanthocyanidin biosynthesis in soybean remain ... | 2017 | 28960323 |
| amplified fragment length polymorphism (aflp) in soybean: species diversity, inheritance, and near-isogenic line analysis. | amplified fragment length polymorphism (aflp) analysis is a pcr-based technique capable of detecting more than 50 independent loci in a single pcr reaction. the objectives of the present study were to: (1) assess the extent of aflp variation in cultivated (gycine max l. merr.) and wild soybean (g. soja siebold & zucc.), (2) determine genetic relationships among soybean accessions using aflp data, and (3) evaluate the usefulness of aflps as genetic markers. fifteen aflp primer pairs detected a to ... | 1996 | 24162296 |
| soybean aphid, aphis glycines (hemiptera: aphididae), developmental and reproductive capacity on white clover, trifolium repens (rosales: leguminosae), in northeast china. | nymphs of aphis glycines matsumura were individually reared to adults in the laboratory on detached leaf discs of trifolium repens l. (white clover) mounted on agar medium. adults of a. glycines were fed t. repens within small clip cages in the field. development, reproduction and intrinsic rates of increase of a. glycines were studied. these data were compared to those of controls fed known host plants including cultivated soybean glycine max (l.) merr. and the wild soybean species glycine soja ... | 2017 | 28798493 |
| comparative analysis of complete plastid genomes from wild soybean (glycine soja) and nine other glycine species. | the plastid genomes of different plant species exhibit significant variation, thereby providing valuable markers for exploring evolutionary relationships and population genetics. glycine soja (wild soybean) is recognized as the wild ancestor of cultivated soybean (g. max), representing a valuable genetic resource for soybean breeding programmes. in the present study, the complete plastid genome of g. soja was sequenced using illumina paired-end sequencing and then compared it for the first time ... | 2017 | 28763486 |
| the difference of photosynthetic responses to the cadmium stress between a wild soybean (glycine soja sieb. et zucc.) and a cultivated soybean. | the effects of cd on a halophyte of doingying wild soybean (dy-03262) were studied by comparing it with a cultivated soybean (sn-11) via investigating photosynthetic activity, chlorophyll fluorescence and cd concentrations in leaves after treatment with different concentrations of cd (0, 50, and 100 μm) for 10 days. there were no significant differences in photosynthetic rate (pn), actual photochemical efficiency of psii (φpsii), stomatal conductance (gs), and photochemical quenching (qp) betwee ... | 2017 | 28752180 |
| mapping novel aphid resistance qtl from wild soybean, glycine soja 85-32. | two novel qtls conferring aphid resistance were mapped and validated on soybean chromosomes 8 and 16, respectively. closely linked markers were developed to assist breeding for aphid resistance. soybean aphid, aphis glycines matsumura, is a highly destructive pest for soybean production. e08934, a soybean advanced breeding line derived from the wild soybean glycine soja 85-32, has shown strong resistance to aphids. to dissect the genetic basis of aphid resistance in e08934, a mapping population ... | 2017 | 28710504 |
| comparison of salt tolerance in soja based on metabolomics of seedling roots. | soybean is an important economic crop that is continually threatened by abiotic stresses, especially salt stress. wild soybean is an important germplasm resource for the breeding of cultivated soybean. the root system plays a very important role in plant salt tolerance. to explore the salt tolerance-related mechanisms among soja, we have demonstrated the seedling roots' growth and metabolomics in wild soybean, semi-wild soybean, and cultivated soybean under two types of salt stress by using gas ... | 2017 | 28690628 |
| a novel ap2/erf family transcription factor from glycine soja, gserf71, is a dna binding protein that positively regulates alkaline stress tolerance in arabidopsis. | here we first found that gserf71, an erf factor from wild soybean could increase plant alkaline stress tolerance by up-regulating h+-atpase and by modifing the accumulation of auxin. alkaline soils are widely distributed all over the world and greatly limit plant growth and development. in our previous transcriptome analyses, we have identified several erf (ethylene-responsive factor) genes that responded strongly to bicarbonate stress in the roots of wild soybean g07256 (glycine soja). in this ... | 2017 | 28681139 |
| seed weight differences between wild and domesticated soybeans are associated with specific changes in gene expression. | our study systematically explored potential genes and molecular pathways as candidates for differences in seed weight resulting from soybean domestication. in addition, potential contributions of lncrnas to seed weight were also investigated. soybeans have a long history of domestication in china, and there are several significant phenotypic differences between cultivated and wild soybeans, for example, seeds of cultivars are generally larger and heavier than those from wild accessions. we analy ... | 2017 | 28653111 |
| extraction optimization, preliminary characterization and antioxidant activities of polysaccharides from glycine soja. | single-factor experiment and central composite design (ccd) was applied to optimize the ultrasound-assisted extraction (uae) conditions of polysaccharides from glycine soja (cgps), and a preliminary characterization of three polysaccharide fractions (cgps, gps-1, and gps-2) and their antioxidant activities were investigated. under the optimal conditions: ratio of liquid to solid 42.7ml/g, extraction power 293.7w, extraction temperature 68.9°c, and extraction time 34.7min, the experimental cgps y ... | 2017 | 28579464 |
| nucleotide diversity patterns of three divergent soybean populations: evidences for population-dependent linkage disequilibrium and taxonomic status of glycine gracilis. | the level of linkage disequilibrium (ld) is a major factor to determine dna polymorphism pattern of a population and to construct high-resolution maps useful in localizing and gene cloning of complicated traits. here, we investigated ld level of three soybean populations with different genetic backgrounds and taxonomic status of g. gracilis by comparing the dna polymorphism patterns of four high-diversity single-copy nuclear genes. a total of 152, 22, and 77 accessions of g. soja, g. gracilis, a ... | 2015 | 26442568 |
| protamine sulfate precipitation method depletes abundant plant seed-storage proteins: a case study on legume plants. | depletion of abundant proteins is one of the effective ways to improve detection and identification of low-abundance proteins. our previous study showed that protamine sulfate precipitation (psp) method can deplete abundant ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) from leaf proteins and is suitable for their in-depth proteome investigation. in this study, we provide evidence that the psp method can also be effectively used for depletion of abundant seed-storage proteins (ssps) f ... | 2015 | 25641780 |
| genome re-sequencing of semi-wild soybean reveals a complex soja population structure and deep introgression. | semi-wild soybean is a unique type of soybean that retains both wild and domesticated characteristics, which provides an important intermediate type for understanding the evolution of the subgenus soja population in the glycine genus. in this study, a semi-wild soybean line (maliaodou) and a wild line (lanxi 1) collected from the lower yangtze regions were deeply sequenced while nine other semi-wild lines were sequenced to a 3-fold genome coverage. sequence analysis revealed that (1) no independ ... | 2014 | 25265539 |
| gschx19.3, a member of cation/h+ exchanger superfamily from wild soybean contributes to high salinity and carbonate alkaline tolerance. | cation/h+ exchangers (chx) are characterized to be involved in plant growth, development and stress responses. although soybean genome sequencing has been completed, the chx family hasn't yet been systematically analyzed, especially in wild soybean. here, through hidden markov model search against glycine soja proteome, 34 gschxs were identified and phylogenetically clustered into five groups. members within each group showed high conservation in motif architecture. interestingly, according to o ... | 2017 | 28842677 |
| allelic combinations of soybean maturity loci e1, e2, e3 and e4 result in diversity of maturity and adaptation to different latitudes. | soybean cultivars are extremely diverse in time to flowering and maturation as a result of various photoperiod sensitivities. the underlying molecular genetic mechanism is not fully clear, however, four maturity loci e1, e2, e3 and e4 have been molecularly identified. in this report, cultivars were selected with various photoperiod sensitivities from different ecological zones, which covered almost all maturity groups (mg) from mg 000 to mg viii and mg x adapted from latitude n 18° to n 53°. the ... | 2014 | 25162675 |
| [progress in genes related to seed-coat color in soybean]. | seed-coat color has changed from black to yellow during natural and artificial selection of cultivated soybean from wild soybean, and it is also an important morphological marker. therefore, discovering genes related to the soybean seed-coat color will play a very important role in breeding and evolutionary study. different seed-coat colors caused by deposition of various anthocyanin pigments. although pigmentation has been well dissected at molecular level in several plant species, the genes co ... | 2012 | 22698739 |
| novel alleles among soybean bowman-birk proteinase inhibitor gene families. | trypsin inhibitors have been found in various animals, plants and microorganisms. there were two types of trypsin inhibitors in soybean including bowman-birk protease inhibitors (bbi) and kunitz inhibitors (kti). the different bbi genes from wild soybean (g. soja) and cultivated soybean (g. max) formed a multigene family. we constructed a cdna library of cultivar 'suinong 14' seed at the r7 growth stage using the smart kit. seventeen contigs or singletons were highly homologous to soybean protea ... | 2008 | 18677596 |
| gsslah3, a glycine soja slow type anion channel homologue, positively modulates plant bicarbonate stress tolerance. | alkaline stress is a major form of abiotic stress that severely inhibits plant growth and development, thus restricting crop productivity. however, little is known about how plants respond to alkali. in this study, a slow-type anion channel homologue 3 gene gsslah3 was isolated and functionally characterized. bioinformatics analysis showed that gsslah3 protein contains ten transmembrane helices. consistently, gsslah3 was found to locate on plasma membrane by transient expression in onion epiderm ... | 2017 | 29243826 |
| genome-wide analysis and expression profiling of pp2c clade d under saline and alkali stresses in wild soybean and arabidopsis. | protein phosphatase 2cs (pp2cs) belong to the largest protein phosphatase family in plants. some members have been described as being negative modulators of plant growth and development, as well as responses to hormones and environmental stimuli. however, little is known about the members of pp2c clade d, which may be involved in the regulation of signaling pathways, especially in response to saline and alkali stresses. here, we identified 13 pp2c orthologs from the wild soybean (glycine soja) g ... | 2017 | 29052008 |
| characterization of the soybean genome using est-derived microsatellite markers. | we generated a high-density genetic linkage map of soybean using expressed sequence tag (est)-derived microsatellite markers. a total of 6920 primer pairs (10.9%) were designed to amplify simple sequence repeats (ssrs) from 63,676 publicly available non-redundant soybean ests. the polymorphism of two parent plants, the japanese cultivar 'misuzudaizu' and the chinese line 'moshidou gong 503', were examined using 10% polyacrylamide gel electrophoresis. primer pairs showing polymorphism were then u ... | 2007 | 18192281 |
| [effects of chilling stress under weak light on functions of photosystems in leaves of wild soybean and cultivatar soybean]. | by simultaneously analyzing chlorophyll a fluorescence transient and light absorbance at 820 nm, and detecting activities of superoxide dismutase (sod) and ascorbate peroxidase (apx), the effects of chilling stress under weak light on functions of photosystem i (psi) and photosystem ii (psii) in leaves of a wild type soybean variety 'zyd00019' and a cultivar soybean variety 'zaoshu no.1' were investigated. the results showed that the functions of both psi and psii were damaged by the chilling st ... | 2007 | 17675757 |
| effect of crystal freezing and small-molecule binding on internal cavity size in a large protein: x-ray and docking studies of lipoxygenase at ambient and low temperature at 2.0 a resolution. | flash-freezing is a technique that is commonly used nowadays to collect diffraction data for x-ray structural analysis. it can affect both the crystal and molecular structure and the molecule's surface, as well as the internal cavities. x-ray structural data often serve as a template for the protein receptor in docking calculations. thus, the size and shape of the binding site determines which small molecules could be found as potential ligands in silico, especially during high-throughput rigid ... | 2006 | 16790932 |
| [segregation distortion of molecular markers in recombinant inbred populations in soybean (g. max)]. | the segregation distortion of 238 molecular markers in a rils population from cultivated/semi-wild soybean cross was analysed. 29.4% of the loci were found with segregation distortion, and direction of deviation was mainly towards cultivated variety changnong 4. rapd markers have a higher distortion rate than other molecular markers. | 2000 | 11192432 |
| differences in the metabolic profiles and antioxidant activities of wild and cultivated black soybeans evaluated by correlation analysis. | wild soybeans are considered a potential resource for soybean domestication and an important source of genetic diversity for soybean crop improvement. understanding metabolite-caused bioactivity differences between cultivated and wild soybeans is essential for designing a soybean with enhanced nutritional traits. in this study, the non-targeted metabolic profiling of 26 soybean varieties, 15 wild black soybeans (wbs) and 11 cultivated black soybeans (cbs), using liquid chromatography-mass spectr ... | 2017 | 28888437 |
| fine mapping of the soybean aphid-resistance genes rag6 and rag3c from glycine soja 85-32. | rag6 and rag3c were delimited to a 49-kb interval on chromosome 8 and a 150-kb interval on chromosome 16, respectively. structural variants in the exons of candidate genes were identified. the soybean aphid, an invasive species, has significantly threatened soybean production in north america since 2000. host-plant resistance is known as an ideal management strategy for aphids. two novel aphid-resistance loci, rag6 and rag3c, from glycine soja 85-32, were previously detected in a 10.5-cm interva ... | 2017 | 28887657 |
| the glycine soja nac transcription factor gsnac019 mediates the regulation of plant alkaline tolerance and aba sensitivity. | overexpression of gshdz4 or gsnac019 enhanced alkaline tolerance in transgenic arabidopsis. we proved that gshdz4 up-regulated both gsnac019 and gsrd29b but gsnac019 may repress the gsrd29b expression under alkaline stress. wild soybean (glycine soja) has a high tolerance to environmental challenges. it is a model species for dissecting the molecular mechanisms of salt-alkaline stresses. although many nac transcription factors play important roles in response to multiple abiotic stresses, such a ... | 2017 | 28884328 |
| plant regeneration of wildglycine species from suspension culture-derived protoplasts. | protoplasts isolated from four-week old cell suspension cultures ofglycine canescens f. j. herm andg. clandestina wendl. were cultured in 8p or modified 8p to a multicellular stage. colonies of 0.5 to 1.0 mm diameter were transferred to solid media for callus growth and regeneration. callus consisted of friable masses with compact green nodular areas. organogenesis of both species occurred primarily from the green nodular areas. shoot buds ofg. clandestina did not mature, but shoots ofg. canesce ... | 1989 | 24232998 |
| production, characterization, and applications of monoclonal antibodies reactive with soybean nodule xanthine dehydrogenase. | seven monoclonal antibodies were produced against soybean nodule xanthine dehydrogenase, an enzyme involved in ureide synthesis. specificity of the seven monoclonal antibodies for xanthine dehydrogenase was demonstrated by immunopurifying the enzyme to homogeneity from a crude nodule extract using antibodies immobilized to sepharose 4b beads. each monoclonal antibody was covalently bound to sepharose 4b beads for the preparation of immunoaffinity columns for each antibody. all seven antibodies w ... | 1986 | 16664749 |