Publications

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ddt residues in tobacco and soybeans grown in soil treated with ddt. 19695387693
the use of raw and extruded soybeans in layer diets. 19695387889
effect of raw soybeans on levels of protein and nucleic acids in the rat pancreas. 19695389757
a comparison of lectin binding in rat and human peripheral nerve.eleven different fluorescein- or peroxidase-conjugated lectins with different sugar-binding affinities were employed to analyze and compare glycoconjugates of rat and human peripheral nerves at the light microscopic level. a majority of lectins showed a distinct binding pattern in different structures of the nerve. lectin binding was similar but not identical in rat and human nerves. limulus polyhemus agglutinin did not stain any structures in rat or human nerves. in both species, all other lect ...19863772078
purification and some properties of a non-haem iron protein from the bacteroids of soya-bean (glycine max merr) nodules.a non-haem iron protein was isolated from an extract of soya-bean nodule bacteroids by a procedure including protamine sulphate and heat precipitation followed by chromatography on deae-cellulose. the purified protein contains non-haem iron and acid-labile sulphur and exhibits a spectrum with a rather broad absorption shoulder in the region 380-440nm and a more prominent peak at 280nm. from sedimentation-velocity measurements an apparent s(20,w) value of 1.3s was calculated. the protein function ...19705529239
nutritional value of soybeans in the diet of depancreatized chickens. 19705530321
correlation analysis of criteria of symbiotic nitrogen. fixation by soybeans (glycine max merr.). 19705535969
effect of raw and heated soybeans on gain, nutrient digestibility, plasma amino acids and other blood constituents of growing swine. 19675624670
biochemical changes in the pancreases of chicks fed raw soybeans and soybean meal. 19675625405
trypsin inhibition, hemagglutination, and intraperitoneal toxicity in extracts of phaseolus vulgaris and glycine max. 19665949799
effect of soybean meal, raw soybeans, corn gluten feed, and urea on the concentration of rumen fluid components at intervals after feeding. 19676069995
[catalytic concentration, multiple forms, and lectin affinity of microsomal enzymes from human tissues: lectins as reagents, ii (author's transl)].we estimated the concentrations, multiple forms, and lectin binding of five microsomal enzymes in particle free extracts from human kidney, pancreas, jejunal mucosa, and normal and cancerous liver. while arylesterase markedly reacted only with concanavalin a, arylamidase, alkaline phosphatase, gamma-glutamyltransferase, and cholinesterase were intensely precipitated by lectins from ricinus communis 120, canavalia ensiformis, triticum vulgare and phaseolus vulgaris s. agglutinins from glycine max ...19816120206
endogenous lectin from cultured soybean cells. chemical characterization of the lectin of sb-1 cells.a lectin has been identified in the cell line, sb-1, originally derived from the roots of glycine max. this lectin, which we shall refer to as sb-1 lectin, was isolated on the basis of its carbohydrate-binding activity (affinity chromatography on sepharose column derivatized with n-caproyl-galactosamine) and its immunological cross-reactivity (immunoblotting with rabbit antibodies directed against seed soybean agglutinin (sba]. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) ...19873584143
relevance of multiple soybean trypsin inhibitor forms to nutritional quality.trypsin inhibitors contribute to the antinutritional component of raw soybean meal by inhibiting vertebrate pancreatic serine proteinases in the small intestine, resulting in a range of deleterious physiological effects in the animal. the variation in the nutritional quality of soybean cultivars stems partly from wide-ranging levels of trypsin inhibitor, and from varying proportions of trypsin inhibitors of two classes--the kunitz and the bowman-birk inhibitor classes. the latter class is better ...19863799285
amino acid sequence of a soybean (glycine max) seed polypeptide having a poly(l-aspartic acid) structure.a polypeptide of mr 4400 was isolated from soybean (glycine max) seeds by extraction with 60% ethanol followed by ion-exchange and reverse-phase chromatography. the peptide contains an unusually high amount of aspartic acid residues (approximately 25%, and hence is designated as soybean aspartic acid-rich peptide). its complete primary structure was determined by conventional methods to be the following. ser-lys-trp-gln-his-gln-gln-asp-ser-cys-arg-lys-gln-leu-gln-gly-val-asn- leu-thr-pro-cys-glu ...19873611081
immunological evidence for gap junction polypeptide in plant cells.a whole cell homogenate prepared from soybean (glycine max (l.) merr. cv. mandarin) root cells (sb-1 cell line) was electrophoresed on a sodium dodecyl sulfate-polyacrylamide gel and transferred to nitrocellulose paper. the nitrocellulose was probed with a monospecific antibody capable of recognizing the mr 27,000 polypeptide of rat liver gap junctions; this antibody was prepared from immune serum raised against gap junctions purified from v79 cells (chinese lung fibroblasts). the immunoblots af ...19873805027
interspecific plasmid and genomic dna sequence homologies and localization of nif genes in effective and ineffective strains of rhizobium japonicum.three strains of rhizobium japonicum were examined for the presence of interspecific conserved plasmid-borne dna sequences and the location of their nif dna sequences. strains 61a76 and 110, which both form effective (nitrogen fixing) nodules on soybeans show very low (24%) total dna sequence homology with each other; strain 61a76 contains one plasmid, and strain 110 contains no identifiable plasmids. strain 61a24 which forms ineffective nodules on soybeans shows relatively high (50%) sequence h ...19816286824
protection against dimethylhydrazine-induced adenomatous tumors of the mouse colon by the dietary addition of an extract of soybeans containing the bowman-birk protease inhibitor.dimethylhydrazine (dmh) induces adenocarcinomas of the colon and rectum in rodents; this animal cancer model system is thought to closely resemble human colorectal cancer. an extract of soybeans containing the bowman-birk protease inhibitor added to the diet of dmh-treated mice resulted in a significant suppression of adenomatous tumors of the colonic mucosa. non-treated mice maintained on the same diet for most of their natural life-span (1.5 years) showed no observable health problems.19854040444
soybean hydrophobic protein. isolation, partial characterization and the complete primary structure.a 9000-mr protein isolated from a 60% ethanolic extract of soybean (glycine max) seeds has been characterized and fully sequenced. the protein consists of 80 amino acid residues with four disulfide bonds. it contains a large number of hydrophobic residues and lacks methionine, phenylalanine, tryptophan, lysine and histidine residues. the protein readily crystallizes from water but is quite soluble in aqueous organic solvents like 95% 1-propanol. it aggregates to form large molecules (above 80 kd ...19873830151
pancreatic proteinases from man, trout, rat, pig, cow, chicken, mink and fox. enzyme activities and inhibition by soybean and lima bean proteinase inhibitors.1. the specific activities of the trypsins and chymotrypsins, measured with synthetic substrates, varied within one order of magnitude. 2. the trout trypsin and chymotrypsin were 5-15 times as efficient in hydrolyzing casein as the remaining animals. 3. the inhibition of total caseinolytic activity in extracts of pancreatic tissue by sbti and inhibitors in crude extracts of raw soybeans varied ten-fold. 4. the animals may be ranked as follows according to the sensitivity of the caseinolytic acti ...19836340948
effect of phytate on aflatoxin formation by aspergillus parasiticus grown on different grains.aflatoxin production by aspergillus parasiticus on corn, soybean, and cottonseed in the absence or presence of added sodium phytate was examined. no variation in aflatoxin concentrations was found in raw, chemically sterilized, or autoclaved soybeans whereas a five-fold reduction in total aflatoxins was found in cottonseed after addition of 330 micrograms sodium phytate to 10 g of autoclaved material. however, phytate did not affect aflatoxin production on non-sterile cottonseeds, although in co ...19854069189
cell surface carbohydrate of leishmania mexicana amazonensis: differences between infective and non-infective forms.the cell surface carbohydrates of leishmania mexicana amazonensis (amastigotes and promastigotes, both infective and non-infective forms) were comparatively analyzed by agglutination assay employing 28 highly purified lectins, and by binding assay using 125i-labeled lectins. among the d-galnac binding lectins, bandeiraea simplicifolia-i, dolichos biflorus, phaseolus vulgaris and glycine max were highly specific for the amastigotes, while that from maclura aurantiaca selectively agglutinated prom ...19863709546
assignment of lectins specific for d-galactose or n-acetyl-d-galactosamine to two groups, based on their circular dichroism.the circular dichroism (cd) spectra of thirteen lectins, most being specific for d-galactose or n-acetyl-d-galactosamine, were compared. two groupings are proposed on the basis of the cd in the near-ultraviolet region. group one comprises the lectins from arachis hypogaea, glycine max, phaseolus coccineus, p. lunatus, p. vulgaris, and vicia villosa, and group two comprises lectins from caragana arborescens, cytisus sessilifolius, dolichos biflorus, griffonia simplicifolia, and wisteria floribund ...19854016573
[inhibitory effect of lectins extracted from arachis hypogea and soybeans on germ cell migration in the embryo of xenopus laevis (amphibia, anura)].effect of injection of arachis hypogea (pna) and soybean (sba) lectins on the migration of xenopus primordial germ cells (pgcs) were investigated. pna inhibited almost entirely the pgcs migration, more than sba. our results indicated that the inhibition of the migration could be a consequence of the lectin-induced alterations of cell surface properties.19836412978
separation of a mouse growth inhibitor in soybeans from trypsin inhibitors. 19705466150
lectin binding patterns in developing rat colon.to evaluate qualitative changes in brush-border and goblet cell glycoconjugates during colonic development, rhodamine-conjugated lectins were applied to tissue from fetal (18-22 day), suckling (1-20 day), and weanling (22-26 day) rats. tissue from the proximal and distal colon of each animal was incubated with triticum vulgaris, arachis hypogaea, glycine max, or ulex europeus agglutinin i. formalin-fixed paraffin sections and unfixed frozen sections were coded, read blindly, and graded from nega ...19873557008
elicitor-induced phytoalexin synthesis in soybean cells: changes in the activity of chalcone synthase mrna and the total population of translatable mrna.rapid changes in the mrna activity encoding chalcone synthase, a central enzyme involved in isoflavonoid phytoalexin synthesis, were induced in cultured cells of soybean (glycine max) after treatment with a glucan elicitor from the cell walls of the fungus, phytophthora megasperma f. sp. glycinea, a soybean pathogen. two-dimensional gel electrophoresis of the in vitro- and in vivo-synthesized chalcone synthase showed that it consisted of a group of proteins of similar molecular weights of about ...19853855251
the bowman-birk inhibitor. trypsin- and chymotrypsin-inhibitor from soybeans.four decades of studies on the isolation, characterization, properties, structure, function and possible uses of the bowman-birk trypsin- and chymotrypsin-inhibitor from soybeans are reviewed. starting from bowman's acetone insoluble factor, designated ai, aa and sbtiaa, the bowman-birk inhibitor (bbi) was found to be a protein molecule consisting of a chain of 71 amino acids cross linked by 7 disulfide bonds, with a tendency to self-associate. bbi possesses two independent sites of inhibition, ...19853886572
aberrant, canavanyl protein formation and the ability to tolerate or utilize l-canavanine.l-canavanine, 2-amino-4-(guanidinooxy)butyric acid, and l-arginine incorporation into de novo synthesized proteins was compared in six organisms. utilizing l-[guanidinooxy14c]canavanine and l-[guanidino14c]arginine at substrate saturation, the canavanine to arginine incorporation ratio was determined in de novo synthesized proteins. caryedes brasiliensis and sternechus tuberculatus, canavanine utilizing insects; canavalia ensiformis, a canavanine storing plant; and to a lesser extent heliothis v ...19873582574
the complete amino acid sequence of trypsin inhibitor de-3 from erythrina latissima seeds.trypsin inhibitor de-3 from erythrina latissima seeds contains 172 amino acids, including 4 half-cystine residues, and resembles the kunitz-type inhibitors. limited hydrolysis of de-3 with trypsin at ph 3 produced two fragments, f1 and f2, containing 63 and 109 amino acids, respectively. amino-terminal sequence studies revealed that f1 was the n-terminal and that f2 was the c-terminal fragment. the complete amino acid sequence of fragments f1 and f2 was then determined on peptides produced by en ...19853902809
[genetic improvement of grain legumes of importance to latin american diet and the soy bean].common beans and soybeans can be considered as the most important food legumes in the latin american nutritional context, each of them presenting specific problems. the production of common beans has not increased in the last decade, and insects and diseases attacks are menacing it. on the other hand, soybeans have shown a tremendous increase in production, becoming rapidly an important export cash crop. however, its use in human consumption is quite restricted, in spite of the efforts of some c ...19846543422
aflatoxin-induced alterations in glycine max, cv. 'essex' root cell membrane protein content.aflatoxins (afts) are hepatocarcinogens, mutagens, teratogens and toxins. isolates of afts-producing strains of aspergillus flavus can grow upon both autoclaved soybeans and to a lesser extent field-grown beans. besides inhibiting the elongation of excised, in vitro cultured soybean roots, the afts can impair the roots' ability to remove [14c]-leucine from a culture medium and to incorporate the amino acid into acid-insoluble, cytoplasmic protein. in this connection, exogenous afts once taken-up ...19853935929
flow cytometric analysis of the binding of eleven lectins to human t- and b-cells and to human t- and b-cell lines.the relative surface binding of 11 lectins to human peripheral blood t- and b-lymphocytes, to molt-4 and jm t-cell lines, and to 6410 and nc37 b-cell lines was determined by flow cytometry. the lectins from lens culinaris (lca), ricinus communis (rca), arachis hypogaea (pna), abrus precatorius (apa), ulex europaeus (uea-f), sarothamnus scoparius (sas-f), helix pomatia (hpa), phaseolus coccineus (l-pha), glycine max (sba), and triticum vulgare (wga) were fluoresceinated and incubated with living, ...19846609053
physiological and pathobiological significance of ocular glycoproteins. i. studies using fluorescein labelled glycine max.cell surface carbohydrates play an important role in several biological, immunological, and neoplastic phenomena including development, growth regulation, cellular locomotion, receptor activation, and tumour metastasis. fluorescein labelled lectins which bind to specific carbohydrate residues in glycoproteins and glycolipids are being increasingly used as chemical probes to study cell components. several different preparations of ocular tissues from human, rabbit, and rat were examined for the d ...19854038883
enzymatic properties of the lipoxygenase from pea seeds.a lipoxygenase preparation was obtained from dried green pea seeds. disc electrophoresis with enzyme staining indicated the presence of only one main isoenzyme corresponding to the isoenzyme pl i according to yoon and klein (j. agric. food chem. 24, 955 (1979)), whereas pl ii was absent. the assay for pea lipoxygenase has been optimized by using a final concentration of 0.53 mm potassium linoleate in the presence or absence of 0.2% sodium cholate. without detergent the rho h optimum was 5.9, in ...19853936491
an immunofluorescent analysis of lectin binding to normal and regenerating skeletal muscle of rat.ten different fluorescein-conjugated lectins of various known sugar specificities were used to study cell surface glycoconjugates of normal and regenerating rat skeletal muscle. in normal muscle, canavalia ensiformis agglutinin, triticum vulgaris agglutinin (wheat germ agglutinin, wga), ricinus communis agglutinin-i, and maclura pomifera agglutinin bound strongly to the endomysial region of the myofibers. no binding was observed in the cytoplasm of the myofibers. other lectins (dolichos biflorus ...19853915936
lectin characterization of gonococci from an outbreak caused by penicillin-resistant neisseria gonorrhoeae.a total of 40 neisseria gonorrhoeae isolates, representing 19 penicillin-resistant isolates (from 8 heterosexual patients and 11 homosexual patients) and 21 penicillin-susceptible isolates (from 15 heterosexual patients and 6 homosexual patients) and obtained from the same geographic area, were examined. lectin agglutination patterns were based on the reactivity of the isolates with the following 14 lectins: concanavalin a, lens culinaris, trichosanthes kinlowii, griffonia simplicifolia i, arach ...19853935658
effects of cone opening, initial moisture content and multiple extrusion on the protein quality of extruded soybean using the brady crop cooker.in the present study, three cone openings (0.133; 0.106, and 0.080 cm) and three initial moisture content values (9%, 15% and 21%) were used as treatments to evaluate their effects on the protein quality of full-fat soybean flour, extruded in the brady crop cooker. the specific volume, protein and oil contents as well as available lysine content characteristic of the final product, were not affected by the treatments used. processing temperatures, however, decreased when the initial moisture con ...19853939487
race:cultivar-specific induction of enzymes related to phytoalexin biosynthesis in soybean roots following infection with phytophthora megasperma f. sp. glycinea.primary roots of soybean [glycine max (l.), cv harosoy 63] seedlings were inoculated with zoospores from either race 1 (incompatible, host resistant) or race 3 (compatible, host susceptible) of phytophthora megasperma f. sp. glycinea (pmg) and the activities of phenylalanine ammonia-lyase (pal), chalcone synthase (chs), isoflavone synthase, and dihydroxypterocarpan 6a-hydroxylase related to phytoalexin (glyceollin) biosynthesis, and of glucose-6-phosphate dehydrogenase (glc-6-pdh) and glutamate ...19863963819
isolation and characterization of a soybean lectin having 4-o-methylglucuronic acid specificity.a new lectin from soybeans having specificity toward the extracellular 4-o-methyl-d-glucurono-l-rhamnans produced by certain strains of rhizobium japonicum has been purified and characterized. isolation was accomplished initially by isoelectric precipitation of contaminating globulins and subsequently by affinity chromatography on partially hydrolyzed glucuronorhamnan covalently coupled to amino-hexylagarose. residual globulins were removed by adsorption of the lectin on concanavalin a-agarose a ...19863964668
a new mitogenic d-galactosephilic lectin isolated from seeds of the coral-tree erythrina corallodendron. comparison with glycine max (soybean) and pseudomonas aeruginosa lectins.the lectin of erythrina corallodendron (caesalpiniaceae) seeds was purified by heating, ammonium sulfate fractionation, and affinity chromatography on acid-treated sepharose. the purified lectin is similar to the soybean lectin in being a glycoprotein of molecular weight around 110 000 - 120 000 and having d-galactosephilic activity. this lectin, like the soybean and pseudomonas aeruginosa lectins, binds to d-galactosamine, n-acetyl-d-galactosamine, alpha- and beta-galactosides as well as to d-g ...19816790142
variability in molybdenum uptake activity in bradyrhizobium japonicum strains.twenty naturally occurring strains of bradyrhizobium japonicum in 11 serogroups were screened for the ability to take up mo as bacteroids from soybean root nodules. the strains varied greatly in their ability to take up mo in a 1-min period. the best strain was usda 136, which had an mo uptake activity of almost 3.0 pmol/min per mg of bacteroid (dry weight). in contrast, the poorest strain, usda 62, had an mo uptake activity of 0.35 pmol of mo per min per mg of bacteroid. there were similarities ...19873473064
purification and properties of protease f, a bacterial enzyme with chymotrypsin and elastase specificities.it has been previously demonstrated that commercial bacterial fibrinolysin (ec 3.4.21.7) selectively cleaves the bond between met-53 and ala-54 in ovine prolactin (199 amino acids). a one-step purification procedure on deae-cellulose for protease f, which is the active component of bacterial fibrinolysin, and properties of the purified enzyme are reported. the enzyme is homogeneous as judged by acrylamide gel electrophoresis. its molecular weight, calculated from gel filtration experiments on se ...19836226244
organization and expression of leghaemoglobin genes.leghaemoglobin genes in soybean (glycine max) are present as a moderately reiterated family of sequences. since there are identical restriction site patterns of these sequences in dna isolated from leaf, root, or nodule tissue, the data suggest that no major changes in the organization or methylation of leghaemoglobin genes occur during their induction. cloned soybean leghaemoglobin-cdna cross hybridized with rna from root nodules of kidney bean (phaseolus vulgaris), and to a lesser extent, of p ...19826282418
isolation and expression of rhizobium japonicum cloned dna encoding an early soybean nodulation function.a first visible step in the nodulation of legumes by rhizobium spp. is the deformation and curling of root hairs. we have identified and cloned dna sequences encoding this function from two strains of rhizobium japonicum (usda 122 and usda 110) with a weakly homologous probe from rhizobium meliloti. root hair curling encoded by the cloned dna fragments was examined on soybeans (glycine soja ) after conjugative transfer of these sequences in broad-host-range vectors to various bacterial genera. p ...19846327649
characterization of the binding of the tc-83 strain of venezuelan equine encephalomyelitis virus to bw-j-m, a mouse macrophage-like cell line.the first step in virus replication is attachment of the virus to the host cell surface. to investigate this process, the binding of tc-83 (the attenuated strain of venezuelan equine encephalomyelitis virus) to bw-j-m (a macrophage-like murine cell culture line) has been characterized. the binding of radiolabelled virus can be blocked by excess unlabelled virus and has a ph optimum in the physiological range. binding is saturable; analysis using scatchard plots or the computerized binding data a ...19836337232
mutagenicity, carcinogenicity and reproductive effects of dibromochloropropane (dbcp).dibromochloropropane (dbcp), a brominated organochlorine nematocide, has been used since the mid-1950s. its primary value was its effectiveness on perennial crops without damaging the plants. dbcp also had less acute toxicity than earlier soil fumigants such as ethylene dibromide. dbcp became widely used on citrus, grapes, peaches, pineapple, soybeans and tomatoes. in central america and israel, banana trees were treated with dbcp. by 1975, 25 million pounds were being produced in the u.s. per y ...19836888412
protease inhibitors: possible anticarcinogens in edible seeds.protease inhibitors, which are common constituents of seeds (rice, beans, and maize) have been shown to inhibit breast, colon, and skin cancers in animal experiments. epidemiological studies have shown that diets rich in these components (ie seed proteins) decrease the occurrence of prostatic, breast, and colon cancers in man. we have demonstrated that a typical purified inhibitor from soybeans, the bowman-birk inhibitor, is excreted in the feces complexed with digestive proteases from the duode ...19836346296
lectin receptors as markers of lymphoid cells. i. demonstration in tissue section by peroxidase technique.the distribution of lectin binding receptors in human lymphoid tissue (lymph node and tonsil) was studied in an effort to identify lectins with potential application as cell markers. using a simple peroxidase technique applicable to formalin-fixed, paraffin-embedded tissue, the binding properties of five lectins were investigated: concanavalin a (con a), arachis hypogaea (peanut agglutinin), lotus tetragonolobus (asparagus pea), glycine max (soybean agglutinin) and bandeiraea simplicifolia. thre ...19846380299
comparison of in vitro and in vivo measurements of dietary ca exchangeability and bioavailability.an in vitro method for measuring dietary mineral exchangeability (miscibility with an extrinsic isotopic tracer) was tested by comparison with in vivo measurements in rats. collards, spinach and soybeans, intrinsically labeled with 45ca, were fed to rats together with extrinsically added 47ca. absorption of both tracers was determined by measuring their concentrations in the femur 2 days after consumption of the labeled test meals. the same intrinsically and extrinsically labeled foods were dige ...19836401805
gossypol in cottonseed products: toxicology and inactivation.the little attention given to the potential of cottonseeds as food for monogastric animals and man is due to the presence of toxic principles in them, especially gossypol. the injury to livestock associated with ingestion of cottonseeds results from the unfavorable physiological effect of gossypol per se, as well as from its adverse effects on the protein nutritive value of the food (mainly by rendering lysine metabolically unavailable). various approaches have been employed to overcome these pr ...19836414436
the role of lectins in blood group serology.many lectins display blood group activity, and extracts from dolichos biflorus (anti-a1), ulex europaeus (anti-h), and vicia graminea (anti-n) seeds provide an alternative to human sera as a source of blood-typing reagents. however, the major application of lectins in blood group serology undoubtedly lies in the recognition and elucidation of red celll polyagglutination. in this respect, lectins from arachis hypogaea (anti-t/tk), salvia sclarea (anti-tn). salvia horminum (anti-tn + cad). dolicho ...19806998652
differentiation of bacillus anthracis and other bacillus species by lectins.bacillus anthracis was agglutinated by several lectins, including those from griffonia simplicifolia, glycine max, abrus precatorius, and ricinus communis. some strains of bacillus cereus var. mycoides (b. mycoides) were strongly reactive with the lectin from helix pomatia and weakly reactive with the g. max lectin. the differential interactions between bacillus species and lectins afforded a means of distinguishing b. anthracis from other bacilli. b. cereus strains exhibited heterogeneity with ...19846418761
purification and characterization of mrna from soybean seeds. identification of glycinin and beta-conglycinin precursors.poly(a)-rich rna was isolated from developing soybean seeds (glycine max (l.) merr.) and fractionated on linear log sucrose gradients. two major fractions sedimenting at 18 s and 20 s were separated and then purified by further sucrose gradient fractionation. both fractions were active as messengers when added to a rabbit reticulocyte lysate protein synthesis system. the 18 s fraction caused proteins migrating primarily to the 60,000-dalton region of a sodium dodecyl sulfate gel to be produced, ...19816167584
radiolabeled iron in soybeans: intrinsic labeling and bioavailability of iron to rats from defatted flour.soybeans can be efficiently labeled with radiolabeled iron by supplying the iron via a nutrient culture medium as an iron salt or as a chelate. by using dual labeled iron and edta, it was determined that none of the chelator was transported to the shoots with the iron. therefore, the use of chelated iron as the iron source in the nutrient medium should not affect assessments of bioavailability of iron from plants. bioavailability (determined from whole-body retention curves of 59fe in rats) of i ...19846427432
a possible mechanism for the cellular coaggregation between actinomyces viscosus atcc 19246 and streptococcus sanguis atcc 10557.the cells of actinomyces viscosus atcc 19246 (av19246) and streptococcus sanguis atcc 10557 (ss10557) coaggregated immediately after mixing in 40 mm-tris/hcl buffer. optimal conditions were ph 7.5 in the presence of ca2+ at 0.1 mm or higher. na2 edta and its analogues, na2mgedta and na2mnedta at 7.5 mm inhibited the coaggregation. trypsin and heat treatment impaired the reactive site on av19246 cells, but not on ss10557 cells. the coaggregates, once formed, dissociated gradually during extended ...19846481336
phytoalexin synthesis in soybean cells: elicitor induction of phenylalanine ammonia-lyase and chalcone synthase mrnas and correlation with phytoalexin accumulation.a glucan elicitor from cell walls of the fungus phytophthora megasperma f. sp. glycinea, a pathogen of soybean (glycine max), induced large and rapid increases in the activities of enzymes of general phenylpropanoid metabolism, phenylalanine ammonia-lyase, and of the flavonoid pathway, acetyl-coa carboxylase and chalcone synthase, in suspension-cultured soybean cells. the changes in phenylalanine ammonia-lyase and chalcone synthase activities were correlated with corresponding changes in the mrn ...19846540068
studies on the inhibition of pancreatic and microbial lipases by soybean proteins.a protein, molecular weight 70,000 that inhibits pancreatic lipase has been isolated from soybean seeds. inhibition is not reversed by colipase unless bile salts are added to the assay system. inhibitory properties of the purified protein are very similar to those of serum albumin or alpha-lactoglobulin. it has been confirmed that, during intestinal lipolysis of dietary fats, bile salts play an essential role for the activation of the lipase-colipase system in the presence of inhibitory proteins ...19846542933
the use of blends of cassava flour and extruded full-fat soybeans in diets for broiler chickens.a study was conducted to determine the effects of blending different levels of a low-prussic acid cassava flour with extruded full-fat soybeans in diets for growing broiler chickens. the full-fat soybeans contribute oil which increases the energy content of the diet, aids in overcoming the dusty nature of cassava, and provide high-quality protein. one-third, two-thirds, and all of the maize was replaced by cassava in diets with none, 12.5 and 25% extruded full-fat soybeans. diets were fed in pel ...19846544063
a survey of the nutritional and haemagglutination properties of legume seeds generally available in the uk.eighty-five samples from fifteen different legume seed lines generally available in the uk were examined by measurements of their net protein utilization by rats and by haemagglutination tests with erythrocytes from a number of different animal species. from these results the seeds were classified into four broad groups. group a seeds from most varieties of kidney (phaseolus vulgaris), runner (phaseolus coccineus) and tepary (phaseolus acutifolius) beans showed high reactivity with all cell type ...19836615758
intracellular occurrence of cowpea mild mottle virus in two unrelated plant species.the filamentous particles of cowpea mild mottle virus (cmmv) were detected only in the cytoplasm of palisade, mesophyll, parenchyma and epidermal cells of glycine max (papilionaceae) and nicotiana clevelandii (solanaceae). they were aggregated to form either sheets or bundles or, more frequently, brush-like inclusions. although cmmv was initially considered to be a possible member of the carlavirus group, it differs from aphid-borne carlaviruses in its intracellular occurrence and transmission b ...19836629699
elements in major raw agricultural crops in the united states. 1. cadmium and lead in lettuce, peanuts, potatoes, soybeans, sweet corn, and wheat. 19836655141
trypsin inhibitor effects on food intake and weight gain in zucker rats.decreased body weight and increased pancreas weight which occur in rats fed raw soybeans are thought to be due to the presence of trypsin inhibitors in the soybeans (sbti). since trypsin is postulated to be a negative feedback signal for cholecystokinin (cck) secretion, sbti may have these effects by increasing secretion of cck. cck is a putative satiety signal; thus, increased secretion of cck could decrease food intake, and, if maintained over a period of time, body weight. in these experiment ...19836657770
bowman-birk soybean protease inhibitor as an anticarcinogen.vegetarian populations show a decreased occurrence of breast, colon, and prostatic cancers. epidemiological studies have identified seeds (maize, corn, and beans) as protective agents in these cancers. we have selected to study one abundant component of all seeds, protease inhibitors. synthetic and natural protease inhibitors have been shown to inhibit tumor promotion in vivo and in vitro. in the present study, we report that a typical, natural protease inhibitor, the bowman-birk inhibitor isola ...19836682011
labeling of soybeans with the stable isotope 70zn for use in human metabolic studies.radiozinc (65zn) was used to assess the efficiency of incorporation of the label into soybean seeds by means of a noncycling hydroponic system. it was found that 21.3 and 27.6% of the applied dose was incorporated into the mature seeds when the tracer was applied during vegetative or flowering stages of growth cycle, respectively. the latter method was adapted to labeling with the stable isotope 70zn, and quantitative incorporation of this isotope was investigated within and among hydroponic cul ...19836682436
iron absorption from legumes in humans.iron bioavailability in a variety of legumes was determined by radioisotopic measurements of iron absorption in human subjects. soybeans, black beans, lentils, mung beans, and split peas were prepared as soups, labeled by the extrinsic tag method, and served to fasting subjects. mean percentage absorption was uniformly low, ranging from 0.84 to 1.91%. differences in absorption between the means were not statistically significant. the results indicate that these five commonly eaten legumes are al ...19846741854
studies on lectins. li. the role of mn2+ in the activity of the soybean lectin.in contradistinction to previous reports, the lectin of soybeans (glycine soja) has been shown to retain its erythroagglutinating activity after complete removal of manganese from its molecule. the applied demetallization procedures (dialysis against 0.1 m hcl or 1m acetic acid and against 0.1 m edta following by dialysis against 1 m acetic acid) had no effect on the stability of the lectin subunit structure, as proved by ultracentrifugation analysis or thin-layer chromatography on sephadex g-20 ...19817197555
evaluation of active versus passive uptake of metabolites by rhizobium japonicum bacteroids.rhizobium japonicum bacteroids were isolated anaerobically from soybean [glycine max (l.) merr] nodules. the bacteroids, which were capable of acetylene reduction and respiration, were used to study the uptake of metabolites by a method which permits correction for nonspecific adsorption of metabolites and estimation of total cell volume. these determinations permit active uptake to be assessed from metabolite accumulation against a concentration gradient. succinate, malate, alpha-ketoglutarate, ...19846203891
lectins as markers of human epidermal cell differentiation.the expression of sugar residues on human epidermal cells was investigated by means of lectin binding, as a way of determining membrane structural changes occurring during the differentiation of the epidermis. fourteen lectins of different sugar specificity were conjugated with fluorescein isothiocyanate (fitc-lectins) and tested in fluorescence microscopy on frozen sections of normal human epidermis. in parallel, fitc-lectins were tested on psoriatic-involved epidermis to visualize differences ...19826816653
effect of microwave heating of soybeans on protein quality.the purpose of this study was to determine the protein quality of microwave cooked soybeans by the rat growth and protein efficiency ratio method (per). it was found that properly cooked, dry, hulled, whole soybean seeds had a per of 2.4 +/- .06 (mean +/- standard error) and a mean weekly weight gain of 21.2 +/- 1.1 which were equivalent to 2.53 +/- .10 and 18.3 +/- 1.0 g for casein, respectively. these data demonstrate the value of microwave cooked soybeans and suggest further research on the p ...19817199277
availability to rats of zinc from soybean seeds as affected by maturity of seed, source of dietary protein, and soluble phytate.male rats were used to assess the availability of zinc in soybean seeds (glycine max, var. 'amsoy') as affected by maturity of seed, dietary protein source, and exogenous dietary sodium phytate. seeds were harvested from either 84- (immature) or 120-day-old (mature) plants grown in 65zn-labeled nutrient solutions that contained either 0.066 or 0.262 ppm zinc as zinc sulfate. increased zinc supply to plants increased the zinc content of seeds; immature seeds had higher concentrations of zinc than ...19827200513
protein quality and oil digestibility of lupinus mutabilis: metabolic studies in children.the nutritional quality of lupins (lupinus mutabilis) for infants and children was evaluated in two sets of balance studies. in the first the digestibility and protein quality of diets based on lupin flour, with and without methionine supplementation, were compared with those of a control diet consisting of casein, sucrose and vegetable oil. apparent nitrogen absorption from lupin flour (81.8 and 84.3% of intake) was slightly but significantly less than that during casein control periods (87.2 a ...19836834148
a soybean lectin having 4-o-methyl-d-glucuronic acid specificity.evidence is presented for the presence of a new lectin activity in soybean seeds [glycine max (l.) merrill] that has specificity towards the 4-o-methyl-d-glucurono-l-rhamnan exopolysaccharide produced by certain strains of rhizobium japonicum. bacterial agglutination and precipitin reactions revealed the lectin activity in phosphate-buffered saline extracts of seeds of all cultivars tested, including the "lectinless" varieties. reaction of such extracts with carbohydrate haptens demonstrated tha ...19836838587
in vitro evaluation of fungicides and antibiotics against macrophomina phaseolina (tassi) goid, the incitant of leaf spot of soybean (glycine max.). 19817309555
soybeans vs. textured soy proteins as meat extenders. cooking losses, palatability, and thiamin content of freshly cooked and frozen meat loaves.the effects of replacing 30 percent of the ground beef in meat loaves with boiled ground soybeans or textured soy protein (tsp) were studied. the substitutions were equally effective in increasing yields of freshly cooked, raw frozen and cooked, and cooked, frozen, and reheated meat loaves. scores for several palatability characteristics, including general desirability, were higher for meat loaves containing soybeans than for loaves containing tsp. the mixtures and loaves contained similar amoun ...19826889616
soybean (glycine max [l.] merrill) as a short-term assay for study of environmental mutagens. a report of the u.s. environmental protection agency gene-tox program.the soybean (glycine max [l.] merrill) spot test is suggested as a preliminary screening test for environmental mutagens. this system makes use of various types of spots that originate from the treatment of seeds or seedlings with mutagens. the homozygous dominant y11y11 dark green leaves may show light green and very dark green spots; the heterozygous y11y11 light green leaves may show dark green, yellow or twin spots; and the homozygous recessive y11y11 yellow leaves show light green receptors ...19826891026
zinc absorption from composite meals. ii. influence of the main protein source.the absorption of zinc in man from composite meals of conventional foods with various main protein sources was measured with a radionuclide technique. the meals were extrinsically labeled with 65zn and the absorption of zinc was determined from a measurement of the whole-body retention of the isotope about 2 weeks after ingestion of the labeled meal. the meals consisted of a meat patty made from either chicken, beef, or soybeans, and included potatoes, tomatoes, and white bread. for meals based ...19806893252
lymphocyte cell surface glycoproteins which bind to soybean and peanut lectins.in cellular immunology, peanut (arachis hypogaea) lectin has been used to selectively agglutinate immature lymphoid cells and soybean (glycine max-lectin to agglutinate b lymphocytes. we have used affinity chromatography to study the surface glycoproteins of rat and mouse lymphoid cells which bind to these lectins. thymocyte and t and b lymphocyte glycoproteins were analysed either without modification (native) or after the removal of sialic acid with neuraminidase (asialo). the only native glyc ...19826980823
mutagenicity of municipal water obtained from an agricultural area.we have studied the mutagenicity of the water from lake bloomington and of the tap water that is made from the lake water. the lake, which is the source of drinking water for bloomington, illinois (pop. 44,000), is surrounded by land that is farmed intensively--being mainly in maize and soybeans. samples were collected monthly from may through october 1979 and concentrated 3,000x with xad-2 resin. nearly all of the lake and tap water concentrates were mutagenic in salmonella typhimurium ta98 and ...19817026225
clotting and other plasma factors in experimental endotoxemia: inhibition of degradation by exogenous proteinase inhibitors.endotoxemia in dogs was induced by a slow intravenous infusion of e. coli endotoxin for 2 h. thereby, a significant decrease was observed in the plasma levels of several clotting, fibrinolysis and complement factors. the changes were studied over an experimental period of 14 h and checked for statistical significance by three-way analysis of variance. application of the broad-spectrum proteinase inhibitor aprotinin (trasylol) from bovine organs clearly lowered the endotoxin-induced decline of th ...19817028486
the effects of soybean trypsin inhibitors on the pancreas of animals and man: a review.human trypsin is more resistant to inhibition than is the trypsin of other mammalian species. the effect on human trypsin of soybean trypsin inhibition in soy protein does not appear to be a potential hazard to man. therefore, the elimination of sti does not seem to be necessary for humans. in animal diets, however, pancreatic toxicity must be considered whenever soybean protein is utilized. soybeans should be treated to increase their nutritional benefits and decrease any animal health risks (2 ...19827036513
biosynthetic control of the natural abundance of carbon 13 at specific positions within fatty acids in saccharomyces cerevisiae. isotopic fractionation in lipid synthesis as evidence for peroxisomal regulation.measurements of the natural abundance of 13c at c-1, c-9, and c-10 in fatty acids synthesized by saccharomyces cerevisiae grown aerobically at 30 degrees c show that alkyl chain positions derived from the carboxyl group of the acetate precursor must be enriched in 13c by 2.5 +/- 0.6 parts per thousand while those derived from the methyl group in acetate must be depleted in 13c by an equal amount. selective depletions of 13c observed at the c-9 and c-10 positions of palmitoleate and oleate requir ...19827040368
soybean trypsin inhibitor(s) reduce absorption of exogenous and increase loss of endogenous protein in miniature pigs.it was the purpose of this study to define whether trypsin inhibitors impair protein digestibility via enhanced loss of exogenous or endogenous protein by quantifying those losses using the homoarginine technique, recently developed in this laboratory. pigs fitted with permanent ileal t-cannulas were fed test meals containing homoarginine-labeled protein. the meals contained casein and increasing doses of trypsin inhibitors (experiment 1) or alternatively either heat-treated or raw ground soybea ...19937505318
pancreatic enlargement is evident in rats fed diets containing raw soybeans (glycine max) or cowpeas (vigna unguiculata) for 800 days but not in those fed diets based on kidney beans (phaseolus vulgaris) or lupinseed (lupinus angustifolius).pancreatic weights and composition were studied with rats fed diets containing raw legume seeds for up to 800 d. rapid pancreatic enlargement was induced by dietary soybeans (glycine max) (high kunitz and bowman-birk trypsin inhibitor contents, moderate lectin content) during the initial 150 d. over the next 200 d the rate of pancreatic growth was similar to that in controls. after 350 d a second period of rapid pancreatic growth occurred. macroscopic pancreatic nodules were evident in a number ...19937505319
use of lectins for detection of glycoconjugates in the glandular cells of the human bronchial mucosa.paraffin-embedded human bronchial biopsies, obtained in macroscopically healthy areas, were examined using nine peroxidase-bound lectins. these were either isolated or purified by affinity column chromatography (ulex europeus, triticum vulgare, glycine max, and arachis hypogatea lectins) or commercial preparations (lotus tetragonolobus, canavalia ensiformis, helix pomatia, phaseolus vulgaris, and lens culinaris lectins) and conjugated to peroxidase (except for concanavalin a). these labeled lect ...19827130674
[effect of protease inhibitors on influenza virus reproduction].natural inhibitors of proteases have been shown to inhibit plaque production by influenza virus. investigation of the fate of viral intracellular proteins revealed the inhibiting effect of these inhibitors on their posttranslation cutting. it is assumed, therefore, that the antiviral effect of the preparation under study is associated with inhibition of viral proteins cutting, in particular, of hemagglutinin. a comparative study of the antiviral activity of inhibitors of proteases from various s ...19827135926
circular dichroism studies on conformational transitions of phytohemagglutinins effected by some alcohols.the effects of methanol, ethanol, 1-propanol and 2,2,2,-trifluoroethanol (tfe) on conformation of 10 phytohemagglutinins (lectins) were examined by applying the circular dichroism (cd) method. concanavalin a and the lectins isolated from pea (pisum sativum), lentil (lens culinaris), soybean (glycine max), peanut (arachis hypogaea), dolichos biflorus, sophora japonica, lotus tetragonolobus, ulex europaeus i, and phaseolus vulgaris (leucoagglutinin) were used. slight changes in the structure of th ...19827150429
the effects of bivalent cations on ribulose bisphosphate carboxylase/oxygenase.the half-saturation constants for binding of the bivalent cations (mg2+, ni2+, co2+, fe2+ and mn2+) to ribulose bisphosphate carboxylase/oxygenase from glycine max and rhodospirillum rubrum were measured. the values obtained were dependent on the enzyme and the cation present, but were the same for both oxygenase and carboxylase activities. ribulose bisphosphate rather than its cation complex was the true substrate. the kinetic parameters vmax.(co2), vmax.(o2), km(co2), km(o2), and k1(o2) were d ...19816796054
specificities of antisera directed against soybean lipoxygenases-1 and -2 and purification of lipoxygenase-2 by affinity chromatography.antiserum directed against lipoxygenase-1 from soybean (glycine max (l). merr. var. williams) was developed in rabbits with electrophoretically pure lipoxygenase-1 as an antigen. to remove traces of lipoxygenase-1 from a lipoxygenase-2 preparation the latter was chromatographed over a column of sepharose 4b to which antibodies directed against lipoxygenase-1 were coupled. during affinity chromatography of lipoxygenase-2 no protein and only a small amount of activity were lost. affinity-purified ...19826816287
lectins and the soybean-rhizobium symbiosis. i. immunological investigations of soybean lines, the seeds of which have been reported to lack the 120 000 dalton soybean lectin.seeds of six soybean lines (glycine max (l.) merr. cv. columbia, d68-127, norredo, sooty, t-102, wilson 5) have been reported to lack the 120 000 dalton soybean lectin. immunodiffusion and radioimmunoassay using anti-soybean lectin immunoglobulin failed to detect the lectin in seeds of five lines, but d68-127 seeds contained as much soybean lectin as the control line, harosoy 63. the d68-127 seed lectin could be purified by affinity chromatography on sepharose-n-caproylgalactosamine, and was ind ...19807190028
nodulation of soybeans carrying the nodulation-restrictive gene, rj1, by an incompatible rhizobium japonicum strain upon mixed inoculation with a compatible strain.the rj1 gene in soybeans prevents nodulation by most strains of rhizobium japonicum. several strains, however, are known to nodulate rj1 plants in vermiculite or sand culture. pure broth cultures of one of these strains (61 nalr) and a strain producing the typical non-nodulating response with rj1 (i-110 ars) were mixed and used as inoculum on clark rj1 soybeans in a growth chamber experiment. both strains carried drug resistance markers and were identified using selective media. analysis of the ...19807190864
effect of raw legume diets on disaccharidase activity in the small intestine of chicks.the effect of four raw legume diets: field beans (vicia faba) (rfb), navy beans (phaseolus vulgaris) (rnb), soybeans (glycine soja) (rsb) and bitter vetch (vicia ervilia) (rbv), on disaccharidase activities in chick small intestine have been studied. maltase and sucrase activities, which vary with age, were determined in 1 to 60 day old animals, rfb and rbv diets had no effect on maltase activity and only increased sucrase activity in 60 day old chicks. both maltase and sucrase activities decrea ...19807192009
effects of ingesting soy or egg lecithins on serum choline, brain choline and brain acetylcholine.rats were fed lecithins, derived from eggs or soybeans, to determine whether the fatty acid composition of the phosphatidylcholine altered choline availability. rats were fed either a single meal containing 5 g phosphatidylcholine or a lecithin-containing diet for 3 weeks, including approximately 5 g phosphatidylcholine per day. each form of dietary lecithin elevated blood choline, brain choline and brain acetylcholine significantly (p < 0.05). there was no difference in response to egg- or soy- ...19817192727
interaction of lectins from soybean and peanut with rhizobia that nodulate soybean, peanut, or both plants.four of 14 strains of rhizobium japonicum from soybean nodulated peanut (arachis hypogaea l. cultivar jumbo virginia), and 3 of 8 rhizobium sp. strains from peanut nodulate soybean (glycine max (l.) merr. cultivar harosoy 63). cells of three peanut rhizobia bound fluorescent- and radioisotope-labeled soybean lectin. two of these strains failed to nodulate soybean, and conversely, two peanut strains that nodulated soybean did not bind to soybean lectin. both culture medium and age had pronounced ...19807195297
soybean diet lowers breast tumor incidence in irradiated rats.this paper examines the relationship between feeding a diet rich in protease inhibitors and the reduction of mammary cancer induced by x-irradiation in sprague-dawley rats. of a total of 145 irradiated animals, 44% of the 45 rats fed a raw soybean diet containing a high concentration of protease inhibitor developed mammary tumors as compared to 74% of 50 rats fed a casein diet containing no protease inhibitor. animals fed purina rat chow which contained low levels of protease inhibitor exhibited ...19807196809
food antibodies in milk from guatemalan women.this study investigates the presence of antibodies against food products in milk samples from 30 guatemalan women of 3 different socioeconomic status: 10 rural poor, 10 urban poor, and 10 urban privileged. the 3 groups had varied dietary habits. both the rural and urban poor mothers do not consume cow's milk. black beans are consumed more often by the urban groups while soy bean products are consumed by both rural and urban poor regularly. milk samples were collected from the mothers. the ...19817196944
the isolation and characterization of a root lectin from soybean (glycine max (l), cultivar chippewa).a lectin has been isolated from the roots of 5-day soybean (glycine max (l) cultivar chippewa) seedlings, and its properties have been compared to those of the soybean seed lectin. the sugar-binding activities of the two lectins, both in terms of specific hemagglutinating activity and sugar specificity, are indistinguishable. molecular properties of the two lectins, measured as relative molecular weights, isoelectric and electrophoretic patterns, amino acid compositions, immunochemical cross-rea ...19817198118
soybean proteinase inhibitors and human proteolytic enzymes: selective inactivation of inhibitors by treatment with human gastric juice.the stability of soybean proteinase inhibitors to 0.1 m hcl and to human gastric juice was investigated. purified kunitz soybean inhibitor (sbti), purified lima bean inhibitor (lbi), and crude, aqueous extract (1:5 w/v) of three varieties of soybeans were incubated for 24 hours at 37 degrees. the sbti, the lbi, and the soybean extracts were mixed with gastric juice or 0.1 m hcl in amounts corresponding to 4.0 g sbti, 2.4 g lbi, and 130 g soybean meal per liter. the trypsin and chymotrypsin inhib ...19817198142
[agronomic, nutritional and processing characteristics of soybean germ plasm with colored seed coats].ten soybean varieties with colored seed coats were evaluated in jaboticabal, with the objective of obtaining information as to color preference in the direct use of soybeans in human consumption. the above-mentioned material showed good adaptation to the local environmental conditions: plant cycles were smaller than the santa rosa (121 days), varying from 107 to 119 days, in spite of the fact that some varieties started blooming later than santa rosa. all materials are within the minimum standar ...19817201302
reduction of the soy-induced inhibition of nonheme iron absorption.recent studies in normal men have demonstrated that soy products have a pronounced inhibitory effect on the absorption of nonheme dietary iron. the purpose of the present investigation was to explore approaches to overcoming this inhibition. a modest but significant increase in iron absorption was observed when test meals containing either isolated soy protein or whole soybeans were baked at 200 degrees c. the addition of 100 mg ascorbic acid to a meal containing isolated soy protein had a more ...19827201739
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